Lag0017484 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017484
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAsparagine--tRNA ligase
Locationchr5: 4225083 .. 4233163 (-)
RNA-Seq ExpressionLag0017484
SyntenyLag0017484
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCGCTATGTCGCCGGCCACTTCTCTCCGCTTCAATCCCTATTCAACTCTCCGTTTCATAGCCGGATTCCCTAAGAAACTACCACAGCGCATTCTTTTCCCGCCAATTCCCCGCCGCCACTCTTCAGATTCTTTCCGGCGAAGTTTTTGCACTGCTGTCTCTGCAGCTCTCTCGTCCGGGGAAGCTGCTGAGAAGACGAAACCTGAAGTTTCCAGAGATTGGAAGGTCGCGCGGGGGGAGAAGGTTGGGGAATTCAGGAAGAAGCTGAGGATCTCTGAAGTAAAGGGCGGCCTCGATGAAGGGCTGGATAGAGTTGGGCAGACGCTTGTCGTCAAGGGCTGGGTTCGGACTCTTCGGGTTCAGAGCAGTGTCACGTTCATGGAGGTAAGATGAGCCAATTCGTGATGGTAGGGACTGGTTTAGAGGCTGAGAAATGCAGGACAGGAAATTTGATATTTTCTTTTCTATTTCCTATTCCGGATCATATGCATTCATATGGTGGTAAATTCTGAATTGGATTGAAGGGTGAGATTGCATTAACCATGTGAGAATTGGCTTGATAATTCTGGGCCGGTCTGGTATTCTTAGAGTTTTAGTGAAAAATCACTCGGCATCGTTGAAGGAACTCCATCGTATCGTATCGTGGAGCTTCTGTTATTAGCATAGTCGGAAATTTTTTTAAAGCTCTGTTTTGCTTCGAGGAGGATTTTGTTGCTTTTAAGAATAAGAAACTAAAGTGGTTGGGGATATGAAACAGAGCCATCTTTGACATTAATTTCAAATGGGACGAAAGAACAAAAAAATGGAAATATGAAGTTGGAGCGTTGGACAGAGGATTAAATAGAGATTGTTGGAAGAAGAAATCTTGTTTTGTATTGTGATGATTATCTTTAATGCCTTGTTTTTTCCTGTGTTTAGGTTAATGATGGCTCATGCATTTCAAATATGCAATGCGTCATGGATTCAACAACGGAAGGTTATGATCAGGTACCCGAGTCAAATAAAATGTTGATCTTAAATAGTTATTGAACTCTGCTTATTTCTGTTTTTGAATGAATGATGTCGATAGCAAGCATATCCATTTTTTTATACAAATAAAATGTTGATCTTGAAGATATTGAACTTCAAATCTAATAGTACATGAGAAGACTGGATTTTAGAAGAAACTGCGTGTGGTTAGAAATATGCGCATTTTTGTTCTATATAACTTGCTTGATTGATAGTGTGATCATCCGCTTTCTCTTTCATGTTCATTTTAGTGGCTTATATTCTTGGTGGATGATCATGAAAGCTGTTAACGAACGGTGACAATAGAGAATGCACATATTTGCACATTTACTATTTATAGATCATGTTATTATCTTTGAGACGACTTTTTTTTTTTAAAAAAAAATGAAAATTTGAAGAAGATCTATAGGTATCTGAACGAACTGTTTTTTTCCGCTCCCTGGAATTTTGTAGTTGAGAGTGTTAATAAGGATTACAAAAAGAGGCTCCATGAACTGTAGCAGATAATTCCAAAAAAATTAATATCATGGACACCGAGAGAGGCATTGGGATTTCTTTTAATATGGTATCTACTTGTCTCTGTCTTCTTATTCCAGCTTTGGAGTTTGTTGGCAGGTTGAATCTGGCTTGATCGCAACGGGTGCATCAGTATGGGTGCAAGGGGTTGTGGTTGCCAGCCAAGGATCAAAACAGAAAGTGGAACTGAAACTCCAAAAAGTTGTTTTGGTTAGTACTTCCCATCCTTTGTGCTCAAGTATGTTTGGCTACAATCTTTTTTCCTGATCACAAACTGCAGTATTTGCTCATATAGTAATATTCCAAAAAAAGGTGGTGTATAGTTTGAACGACACAAAAAAAATGTTATAAAAAATTGGATTTTAAGGAAAGTGAAAATAGGAAATAGAGCGGTCTTCAAGCAAGTCTTGGACATTTGCATCAATAACTTGCTCCTTGTCTTTGTCAATAAATAAAAATGCAAACACAGTTTCTTTCAATGAGGTAAAAGTTGAGGTGTTGAATCTACCTAGATTCACGCTCTATTCATGGCCGATAAATTATATTTGATATCATGTACTTCATAGATTGCATCATAGATGTGCAAATTCATAAAACATTTCCCCTCTACTTGTGTTAATGTTTTCTTTATTTCAGGTTGGCAAGAGTGATCCTTCCTATCCCGTCCAAAAGAAAAGAGTTAGCAGAGAATTTTTGAGAACTAAAGCTCATCTTCGTCCAAGAACAAACACTTTTGGGGCGGTATAGTTCTCTTTTTTATTCAGTTTAGATTCTAGAATACAAGCCTAGGGTGCTAATCCTGATATTGATGAAAAATAAAGGTTGTAATTGTCTAATTTACTAAAATGAACTATTTTGATATCCTCTTTTCATATGTCCTCGTGGTTTGAATTGTTGGAGATCATTTTTCATAGCACTTGTATTGTTCTTGGATCTTTCATAGGTTGGCAAAAGAAAACATGACGATTCACTCTGTGGACATAGTTTTAGAGGGAAAAGTAAAACACTGGATGATTTCTGTGAAAGCAATTCTACGGAGTTTGTGTTTGGAAATGAATTCTCGTCTATTTAATAAAAGGAAGGAGAATGGATGAGGTCTTTGGTGTGGCAAAATTTCAAGCTCTTTTTGTCTAATTTAACTTAAAATTGATGTGCTTTTTGTAAAGTTTGAAAGGGAAGTTACATCATCAATAAAAAAAATTTACGTTTCCCTTCTGCAGGGGAGGATTCCTCAACATCTTGACACAATCTAAGTTTTTTGTTAGAAGATCTTTTAATTTTACTTTCCCTATCTCTGGTGGTAATTATTTATGGATCTCCTCTATGCAAGTTAATTGTCACGTTGTTTCCTGATAATTGGTAACTGAACATAATAATTTAGTCAGATAATTTTTTCACTAGCTTCCCTTTAGTAATTTCATGTGAACGGTTGTCTAATTTATCATTTTGGATATTATGGTGTCGTGTAGGTTGCAAGGGTGAGGAATGCTTTGGCATATGCTACACACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTCTCAAGTCCCATTATTACAGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGTGTGACTACTTTGGTGGGCGTTTTAACTTAGAAAAGCCTGGAATTTGTATGGTCTATAGAATATTTTAGCAAAACAATAGAATATTTAAAAAAAGAATAAAAAGACATTTTCTGATGTTGGCAGATTCCAAGCTCTGGAGAAGCTACCAATTCTGATGTCGACTCTATTCCAAAGACAAATGAGGGATTTATTGATTGGTCACAGGTGAATTAGCCTTGCTAATTTTGGAATCAACCTCTTGATGAATCTAGTTATATTATGTGTCAAGTCAACCATCTCTTTGATTCTTACAGATAAAATTTATGGAAGAAAGATGATATGAGTCATGACCACACACTTTCTTTGGACCTCCTCATTTTCTTCGCAAGTATTTATTTATTCTTTTTCCACTTCTGTTTTAAGGACTTTTTCGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAATGCTGAAACTTATGCTACTGCCCTCTCTGATGTATGGTTTCTCTTCTACACTGGTTCTTGTTGAGGCATGATGGTGGATTTTTTACAAACTGCTAATAATTTGATCTAAACTCTTCTCAGGTTTATACTTTTGGACCGACATTTAGAGCAGAAAATTCCAATACATCTAGGCATTTAGCTGAATTTTGGGTAACTAGTATTCACATTTGTTTAATTTCCTACCAAGTTCAGTTTTACAAGTTATCATATGCGGTAAAAAGCATCTTAAAAAAACCCTTTTACTTTGTTCTTTAGATGATTGAACCGGAACTTGCATTTGCTGATTTGGATGACGACATGGCTTGTGCAACTGCATATCTCCAGTATGTAGTATGTTTCCTTTCATGACTATTGTGTTGTCTTACACTAGAGTTGATTGACAAATATTTGGAATAAAAGTTCTATATTTTATTACTTGACTAAAGATTAACGAAAGCTTTTATTTCCAGGTGAGACACATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACATGGATTGAGAAAGGAATTATTGATCGACTGACTGTAGGAACTCTTACTATTACTCCTAGTCCTTACATTTATTCCTTTAATATATAGTATCTGTTGAAACTTTATATTTACATCTATCATCTATGCCATTTTACCATTGCCATAACTTTTTTTATTTTTGTGTGTTCTGCAGAACCTGGTTGAAAAAGATTTTGTGCAGTTGACTTATACTGATGCAATTGAGCTTCTTCTTCGAGCAAAAAAGAAGTTTGAATATCCGGTAGTGCCCATCATTTTTATTACACCTATGGAAGTTTTGGTTCCATCTCCCATTTATTCTCTGTGATTCTATCTTTTGCATATTGGTTAGATATTCTGGTAGAACTCGTTTATTTACATAGAGAGGAACGTACAGTACCAAAATATAATTTGAGAGATTCTTAAACTCTCTTGACTCCCAATCTAACAAGAATTTAAGAAGTACCCACTGTCTGCCATGGTTACCTTCAAAACAGTAACTGAGGTCACTTTTTTTCAAGCATGATCAGTTACGGTGGACCCTGCACTAATTCTAACTACTTAGCAGATATATTCCTACCCCTCTTACAACCATACACTTTCATAATCAATGGCCCGTCAAATTCTATACTGGAGACAAACTGTTAAAATGGATTCTGTGTTGAATAGCACTTAAATATATTTTTTTGTTTCTTACTAATCTAGTTTGGGTTTGGCGCATTATAGCATTGATATGTAGTCAAGTTGCTTTCATGAGTGTTGTCACTTGTTCTTCCCTCTCAAATTGTTGCCATATTATCATGCCAAGTTTGTGCATTTATTTTCTTTCTCCCCAGAAAGGTTATCAGATTTAACTGTCCTTGTTTTGACCAATGACCATGTTGCGGACTTTCTTAGGAATCTCTGCCTTGCCACTGGTGCTAATTAATCAAATGGCCAAGTATAGTCATTGCTGTTCATTATTATGTGGCCATTGCTGTGCTGATCTCAAACTTTGTAAAATTTTCTTAGGTCAAGTGGGGAAGTGATTTACAAAGCGAACATGAGCGTTATGTAACTGAGGAGGCATTTGGTGGATGCCCTGTAATAATCAGAGACTATCCAAAGGTATGCTTTTCTTTTCGCTTCTAATCTTGCGAGCTCTGATCAAAGAAATTAACTCTGATTTTTCCGGTTGATGGGAAATTATAGGAGATAAAAGCATTCTACATGCGGCAAAATGATGATGGGAAGACTGTTGCGGCCATGGATATGTTGGTTCCTCGGGTAATGTTAAAGTTGTAATGTATATCTCTTTACCTTCCAATAGGGAAGTTTTTTTTTTTTTTTTTGGTGTGTAACTATTTGAAGGAATTGACTGCCAGGTATCAGACTTAGGAACGGTATGAATGGTTGGGGAGAAAAGTCCAATGGGTTATAAGATTTCCAATTTTTTATTCTTATGTCAATCCAATTTTTTTTTGAGATAGAGATTCCTTTTTCCATATCATTATTTTTTTTAAAAAAAATAATTATTATAAGAAACCAAAGTTTTATTGAGAAAAATGAAAGAAGGGTTGTTTCTACACTATCCCTATATTGTAAAAGGCCGGGAATGCAATTGAAGAACTTTTTTGATATGTCTAAGAATACTGACAAGTAATTGTTGCAATTTGAGGCAATAGTTGATGCAAGATAAAGCCATTTCTACAACTAAACATCATCTTTATTGTCATGTGGGGCTGCTTTCTCTCTGTTAAATGCCTTAATTACTGTTATCGCGTCAAGAAAATTTATTTGTTACTGGGTTTAGATTACAGTTGTTTCCTAAGATAAGTTGAGTTCCAATTACTTGTATTCGGAAAAGAAATAGTGACTTCCTCCCATCTTCCTTCTTTCTACCAAACTGAAACAGTTGGCAACCTGTTATTTTTCACTAACCATTAATAATGTCCAAAGAGAAGTTAATAACTTTCCCAGATTTTCTCTTCAATTTTTTTATGTGAAACGGTGTGTGTGTATAACTAATTTAGTAGCTTTCCAAGAGCTTGACTATAAACATGCAATTATATATTTGTAATTTATTTTTCTTGAGATTACTTCTGAAATATGAGAGAAACTTCTTTGACTCTACAATTTGTTTACTGTTAAAGTGAAGGTACTACATAGAGTTATATTTTTCCCATCCTCTCTCTCTTCAGATAGGGGAACTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATCTGGAAGCTCGTCTGGATGACTTGAAACTCAATAAAGACAGCTACTGGTGGTACCTTGATTTGCGTCGTTATGGTTCAGGTTAGAGCTGAAGTCCTTACACTTATGATATTAACTATTTTGTTTTGTGATTAACAAAAAAAGGGAAAGAAAAAATGAAAGAAGGAAAACTTTTAATTGTTTAGAATAATGATTTTCTTTTTCTTTCCATTTTTCAATAAGAAGACAAATGAAAGTTAAAAGATAGGGGCATTAAAAAGGAAACGCCCTAACAAAGTTGCAAACAAAACAAAAGCAAATTACAAAAAATTGCTTTCATTGTTATGAATTAAAAATAAAAAATAATTACATAACCTTGTAGACAAGAATGCCTCAGAGAGAGGCATCAAAATGTATCACATTTGAAACGTCCTCTTAAGAACTCTTCAATTTCTCTCCAGCTAAATATTCTATATGATAGTGAAGAAAGCCATGTGCTAAAGAGCCTGACCTCTTTCCTAACAAGTACCTCCTCCAACATATCCTCGCAACCTCTGAGAGTACCGATTGAAAGAAGGGTGGAGGACAAGAGGGATAGAATGAGAAATTAGGTTGTCAAATTGACTCAGAAGAAAAAATGCCAAGGGGGCCGAGACTTTGCCTAAATGAGTCATTGATATTTGGAATGAGGCAAACAATTAGTAATTCAGTATCGAAAGGAAATTAGACAAATCATGAGATGCAACATCTCAAAGAGGCCTCTTGAAAATTAAATCGAAGTCAAGAGGAAAGGAAAGAATCATCATTATGACATAACACTGAAATAACCAAAGCCTCCTTTCCAAGGAAAAGATTAAAGAGGTGGGGAAAGGAATTGCTAAGGCAGCCATTACCTATCAATAAATTTTTCTGGAAGCCCCATTACCCAAAGGAATGTGATGTAGTTATTAAAGAAAGGTAAGAGAACATGGCTTTTCAAGGACTTCTGGACATGCCTTTAAAGACCACAAACATGATCCACCCTAAGGTTTGACGACCATACTTACAATGAACCTATTGCTACAAATAATTAGGAATCAGGCTCTGAGTGGGATACGCTGAACTTTATGGTTAGTAATGATATGCTAATATAGGTAAATGGCCGAATCTATTTCTTAATGCAATTGAATCTTTTAACCTATTATAGAAATTTTGAGGATTCATCACATGAAAATCTTGTTTAAGCCACCTATTTGAGGATGAGATACCAAGGTGGTCTTTTGATTTCTTCACTGTATACAATACAAGAAGATGAGATCTTCTAAAGCCCAAAATTTTTGCGCCATCTGTGATGAATCTTTATCGATTTCTTAGCTGGGAAATGATGCAAACGTGCAGAACTCCATAACTTTTGTAGAAGGAGAAATATTTTGCACATACTATAGATCAAGAAATTTTGATGATTGTATCAAATTTCACAAATCTGATGGAAGGGAGCTAGTAATGATGTAGGCAGTAAACAAACTTACGAGCTTATGGCATTTGTGAGCTTCGATGATTTTAGCCAATTTCTGATCGTATTGATTTCAAGCAATAAACTAAACTTCAGTGCCTTGAAAAGAAAAAAAAGAGAGAACTTTCTACATTGTAATCAAACGTATTGTGTTTCAGTTCCCCACGCAGGCTTTGGTTTGGGCTTTGAAAGACTTGTGCAGTTTGTTACTGGAATAGATAATATAAGAGATGCCATCCCATTTCCTCGTTCGCCTGGTTCTGCAGAATTTTAA

mRNA sequence

ATGGCTGCCGCTATGTCGCCGGCCACTTCTCTCCGCTTCAATCCCTATTCAACTCTCCGTTTCATAGCCGGATTCCCTAAGAAACTACCACAGCGCATTCTTTTCCCGCCAATTCCCCGCCGCCACTCTTCAGATTCTTTCCGGCGAAGTTTTTGCACTGCTGTCTCTGCAGCTCTCTCGTCCGGGGAAGCTGCTGAGAAGACGAAACCTGAAGTTTCCAGAGATTGGAAGGTCGCGCGGGGGGAGAAGGTTGGGGAATTCAGGAAGAAGCTGAGGATCTCTGAAGTAAAGGGCGGCCTCGATGAAGGGCTGGATAGAGTTGGGCAGACGCTTGTCGTCAAGGGCTGGGTTCGGACTCTTCGGGTTCAGAGCAGTGTCACGTTCATGGAGGTTAATGATGGCTCATGCATTTCAAATATGCAATGCGTCATGGATTCAACAACGGAAGGTTATGATCAGGTTGAATCTGGCTTGATCGCAACGGGTGCATCAGTATGGGTGCAAGGGGTTGTGGTTGCCAGCCAAGGATCAAAACAGAAAGTGGAACTGAAACTCCAAAAAGTTGTTTTGGTTGGCAAGAGTGATCCTTCCTATCCCGTCCAAAAGAAAAGAGTTAGCAGAGAATTTTTGAGAACTAAAGCTCATCTTCGTCCAAGAACAAACACTTTTGGGGCGGTTGCAAGGGTGAGGAATGCTTTGGCATATGCTACACACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTCTCAAGTCCCATTATTACAGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGTGTGACTACTTTGATTCCAAGCTCTGGAGAAGCTACCAATTCTGATGTCGACTCTATTCCAAAGACAAATGAGGGATTTATTGATTGGTCACAGGACTTTTTCGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAATGCTGAAACTTATGCTACTGCCCTCTCTGATGTTTATACTTTTGGACCGACATTTAGAGCAGAAAATTCCAATACATCTAGGCATTTAGCTGAATTTTGGATGATTGAACCGGAACTTGCATTTGCTGATTTGGATGACGACATGGCTTGTGCAACTGCATATCTCCAGTATGTAGTGAGACACATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACATGGATTGAGAAAGGAATTATTGATCGACTGACTAACCTGGTTGAAAAAGATTTTGTGCAGTTGACTTATACTGATGCAATTGAGCTTCTTCTTCGAGCAAAAAAGAAGTTTGAATATCCGGTCAAGTGGGGAAGTGATTTACAAAGCGAACATGAGCGTTATGTAACTGAGGAGGCATTTGGTGGATGCCCTGTAATAATCAGAGACTATCCAAAGGAGATAAAAGCATTCTACATGCGGCAAAATGATGATGGGAAGACTGTTGCGGCCATGGATATGTTGGTTCCTCGGATAGGGGAACTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATCTGGAAGCTCGTCTGGATGACTTGAAACTCAATAAAGACAGCTACTGGTGGTACCTTGATTTGCGTCGTTATGGTTCAGTTCCCCACGCAGGCTTTGGTTTGGGCTTTGAAAGACTTGTGCAGTTTGTTACTGGAATAGATAATATAAGAGATGCCATCCCATTTCCTCGTTCGCCTGGTTCTGCAGAATTTTAA

Coding sequence (CDS)

ATGGCTGCCGCTATGTCGCCGGCCACTTCTCTCCGCTTCAATCCCTATTCAACTCTCCGTTTCATAGCCGGATTCCCTAAGAAACTACCACAGCGCATTCTTTTCCCGCCAATTCCCCGCCGCCACTCTTCAGATTCTTTCCGGCGAAGTTTTTGCACTGCTGTCTCTGCAGCTCTCTCGTCCGGGGAAGCTGCTGAGAAGACGAAACCTGAAGTTTCCAGAGATTGGAAGGTCGCGCGGGGGGAGAAGGTTGGGGAATTCAGGAAGAAGCTGAGGATCTCTGAAGTAAAGGGCGGCCTCGATGAAGGGCTGGATAGAGTTGGGCAGACGCTTGTCGTCAAGGGCTGGGTTCGGACTCTTCGGGTTCAGAGCAGTGTCACGTTCATGGAGGTTAATGATGGCTCATGCATTTCAAATATGCAATGCGTCATGGATTCAACAACGGAAGGTTATGATCAGGTTGAATCTGGCTTGATCGCAACGGGTGCATCAGTATGGGTGCAAGGGGTTGTGGTTGCCAGCCAAGGATCAAAACAGAAAGTGGAACTGAAACTCCAAAAAGTTGTTTTGGTTGGCAAGAGTGATCCTTCCTATCCCGTCCAAAAGAAAAGAGTTAGCAGAGAATTTTTGAGAACTAAAGCTCATCTTCGTCCAAGAACAAACACTTTTGGGGCGGTTGCAAGGGTGAGGAATGCTTTGGCATATGCTACACACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTCTCAAGTCCCATTATTACAGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGTGTGACTACTTTGATTCCAAGCTCTGGAGAAGCTACCAATTCTGATGTCGACTCTATTCCAAAGACAAATGAGGGATTTATTGATTGGTCACAGGACTTTTTCGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAATGCTGAAACTTATGCTACTGCCCTCTCTGATGTTTATACTTTTGGACCGACATTTAGAGCAGAAAATTCCAATACATCTAGGCATTTAGCTGAATTTTGGATGATTGAACCGGAACTTGCATTTGCTGATTTGGATGACGACATGGCTTGTGCAACTGCATATCTCCAGTATGTAGTGAGACACATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACATGGATTGAGAAAGGAATTATTGATCGACTGACTAACCTGGTTGAAAAAGATTTTGTGCAGTTGACTTATACTGATGCAATTGAGCTTCTTCTTCGAGCAAAAAAGAAGTTTGAATATCCGGTCAAGTGGGGAAGTGATTTACAAAGCGAACATGAGCGTTATGTAACTGAGGAGGCATTTGGTGGATGCCCTGTAATAATCAGAGACTATCCAAAGGAGATAAAAGCATTCTACATGCGGCAAAATGATGATGGGAAGACTGTTGCGGCCATGGATATGTTGGTTCCTCGGATAGGGGAACTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATCTGGAAGCTCGTCTGGATGACTTGAAACTCAATAAAGACAGCTACTGGTGGTACCTTGATTTGCGTCGTTATGGTTCAGTTCCCCACGCAGGCTTTGGTTTGGGCTTTGAAAGACTTGTGCAGTTTGTTACTGGAATAGATAATATAAGAGATGCCATCCCATTTCCTCGTTCGCCTGGTTCTGCAGAATTTTAA

Protein sequence

MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALSSGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQKVELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLLRAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF
Homology
BLAST of Lag0017484 vs. NCBI nr
Match: XP_023005353.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 538/566 (95.05%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPA SLRFNPYS LRFI  FPKKLP  ILFPPIPRRHSSD FRRSF TAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPYILFPPIPRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLRISEVKGGLDEGLDRVGQTL+VKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEA NSDV SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVEKDFVQLTYTDA++LLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVKLLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFE+PVKWGSDLQSEHERY+TEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Lag0017484 vs. NCBI nr
Match: XP_022948254.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] >KAG6596861.1 Asparagine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028390.1 Asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 538/566 (95.05%), Postives = 546/566 (96.47%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPA SLRFNPYS LRFI  FPKKLP  ILFPPI RRHSSD FRRSF TAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPHILFPPISRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLRISEVKGGLDEGLDRVGQTL+VKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEA NSDV SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVEKDFVQLTYTDA+ELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFE+PVKWGSDLQSEHERY+TEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Lag0017484 vs. NCBI nr
Match: XP_022154023.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 533/566 (94.17%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPA SLRFNPYSTLRFIAGFP+KLPQ I  PPI RRHSS SFRRSF  AVSAALS
Sbjct: 1   MAAAMSPAISLRFNPYSTLRFIAGFPRKLPQSIPLPPITRRHSSGSFRRSF-RAVSAALS 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEA EKTKPEVS+DWKV RGEKVGEFRKKLR+SE+KGGLDEGLDRVGQTLVVKGWVRTL
Sbjct: 61  SGEAVEKTKPEVSKDWKVTRGEKVGEFRKKLRVSEIKGGLDEGLDRVGQTLVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           R QSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLI TGASVWV GVVVASQGSKQK
Sbjct: 121 RFQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLITTGASVWVHGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VG+SDPSYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF
Sbjct: 181 VELKLQKVVMVGESDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVW+SSPIITASDCEGAGEQFCVTTLIP SGEA NSDV +IPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWISSPIITASDCEGAGEQFCVTTLIPGSGEAANSDVGAIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DMACATAYLQYVVRH+LENCKEDLDFFNTWIEKG+IDRL NLVEKDFVQLTYTDAIELLL
Sbjct: 361 DMACATAYLQYVVRHVLENCKEDLDFFNTWIEKGVIDRLNNLVEKDFVQLTYTDAIELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFE+PVKWGSDLQSEHERY+TEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDS+WWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSFWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 565

BLAST of Lag0017484 vs. NCBI nr
Match: XP_023540678.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 537/566 (94.88%), Postives = 545/566 (96.29%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPA SLRFNPYS LR I  FPKKLP  ILFPPIPRRHSSD FRRSF TAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLRISEVKGGLDEGLDRVGQTL+VKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEA NS V SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVEKDFVQLTYTDA+ELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFE+PVKWGSDLQSEHERY+TEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Lag0017484 vs. NCBI nr
Match: XP_038904123.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1071.2 bits (2769), Expect = 3.0e-309
Identity = 528/566 (93.29%), Postives = 545/566 (96.29%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPATSLRFNP STLRFI G P+KLPQ ILFPP+ RRHSS  FRR+FCTAV    S
Sbjct: 1   MAAAMSPATSLRFNPCSTLRFITGLPRKLPQHILFPPVSRRHSSGYFRRTFCTAV----S 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEAAEKTKPEVS+DWKV RGEKVGEFRKKLRISE+KGGLDEGLDR+GQT VVKGWVRTL
Sbjct: 61  SGEAAEKTKPEVSKDWKVTRGEKVGEFRKKLRISEIKGGLDEGLDRLGQTFVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DST EGYDQVESGLI TGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTIEGYDQVESGLITTGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQK+V+VGKSDPSYPVQKKRVS+EFLRTKAHLRPRTNTFGAVARVRNALAYATHKF
Sbjct: 181 VELKLQKLVVVGKSDPSYPVQKKRVSKEFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSS EA +SDV SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSEEAASSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DM+CATAYLQYVVRHILENCKED+DFFNTWIEKGIIDRLTNLVE DFVQL+YTDAIELLL
Sbjct: 361 DMSCATAYLQYVVRHILENCKEDMDFFNTWIEKGIIDRLTNLVENDFVQLSYTDAIELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFEYPVKWGSDLQSEHERY+TEEAFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEYPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 562

BLAST of Lag0017484 vs. ExPASy Swiss-Prot
Match: O48593 (Asparagine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SYNO PE=2 SV=3)

HSP 1 Score: 894.0 bits (2309), Expect = 8.5e-259
Identity = 443/573 (77.31%), Postives = 497/573 (86.74%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRR-------HSSDSFRRSFCT 60
           MAA   PATSLR    STLRF++ F    P   LF P+ RR         ++S RR FCT
Sbjct: 1   MAATFLPATSLRLTQNSTLRFLSFFTISNPSYSLFRPLRRRVLPPFDAFPANSRRRCFCT 60

Query: 61  AVSAALSSGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVV 120
           AVS +L SG+  +         ++   G KVGEFRKKLRI+EVKGG DEGL RVGQ+L +
Sbjct: 61  AVSESLGSGDGNK------VESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNI 120

Query: 121 KGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVA 180
            GWVRTLR QSSVTF+E+NDGSC+SN+QCVM S  EGYDQVESG I TGASV VQG +VA
Sbjct: 121 MGWVRTLRSQSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVA 180

Query: 181 SQGSKQKVELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNAL 240
           SQG+KQKVELK++K+++VG+ D SYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRN L
Sbjct: 181 SQGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTL 240

Query: 241 AYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGF 300
           AYATHKFFQE+GFVWV+SPIITASDCEGAGEQFCVTTLIPSS E T++ +D+IPKT  G 
Sbjct: 241 AYATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHENTDTSIDAIPKTKGGL 300

Query: 301 IDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360
           IDWSQDFFGKPAFLTVSGQLN ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL
Sbjct: 301 IDWSQDFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360

Query: 361 AFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYT 420
           AFADLDDDMACATAYLQYVV+++L+NCKED++FF+TWIEKGII RL+++ EK+F+QL YT
Sbjct: 361 AFADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYT 420

Query: 421 DAIELLLRAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDD 480
           DAIE+LL+A KKF++PVKWG DLQSEHERY+TEEAFGG PVIIRDYPKEIKAFYMR+NDD
Sbjct: 421 DAIEILLKANKKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDD 480

Query: 481 GKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAG 540
           GKTVAAMDMLVPRIGELIGGSQREERL+ LEARLD+LKLNK+SYWWYLDLRRYGSVPHAG
Sbjct: 481 GKTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAG 540

Query: 541 FGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           FGLGFERLVQFVTGIDNIRD IPFPR+P SAEF
Sbjct: 541 FGLGFERLVQFVTGIDNIRDVIPFPRTPASAEF 567

BLAST of Lag0017484 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.6e-164
Identity = 279/478 (58.37%), Postives = 364/478 (76.15%), Query Frame = 0

Query: 90  KLRISEVKGGLDEGLDRVGQTLVVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTE 149
           + +I  ++G   E    +G  + +KGWVRT+R Q + TF+E+NDGS +SN Q +      
Sbjct: 2   RTKIKSLRGTTPEVRALIGHDITLKGWVRTVRNQKTFTFIEINDGSTLSNFQIIATPDIA 61

Query: 150 GYDQVESGLIATGASVWVQGVVVASQGSKQKVELKLQKVVLVGKSDPS-YPVQKKRVSRE 209
           GYDQ+ + L +TG SV   G +V S G +Q +E++   + ++GK DP  Y +QKKR + E
Sbjct: 62  GYDQLINQL-STGVSVSAIGTIVESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHTFE 121

Query: 210 FLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCV 269
           FLR+ AHLRPRTNT GAV RVRNALA+ATH+FFQ+ GF+++ +PIIT SDCEGAG+ F V
Sbjct: 122 FLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMFQV 181

Query: 270 TTLIPSSGEATNSDVDSIPKTNEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTF 329
           TTL          D ++  +T EG +D++QDFFGKP +LTVSGQLN E YA ALSDVYTF
Sbjct: 182 TTL----------DQNNPARTPEGRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVYTF 241

Query: 330 GPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFN 389
           GPTFRAENSNTSRHLAEFWMIEPE+AFADL+D+M CA  YL+Y+++++L+NC+ED++FFN
Sbjct: 242 GPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEFFN 301

Query: 390 TWIEKGIIDRLTNLVEKDFVQLTYTDAIELLLRAKKKFEYPVKWGSDLQSEHERYVTEEA 449
             +   +I RL +++   F + +YT A+ +L +A KKFEYPVKWG DLQSEHER++ EE 
Sbjct: 302 KHVATDLISRLEHVINTSFERASYTYAVRILEKADKKFEYPVKWGLDLQSEHERFLAEEF 361

Query: 450 FGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLD 509
           FG  PVI+ DYPK+IKAFYMR N+D KTVAAMD+LVP++GE+IGGSQREERL  LE++L 
Sbjct: 362 FGK-PVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLK 421

Query: 510 DLKLNKDSYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           +  L  + YWWYL+LR++GSVPH+GFG GFERLVQF TG++NIRD IPFPR PG A+F
Sbjct: 422 EFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKADF 467

BLAST of Lag0017484 vs. ExPASy Swiss-Prot
Match: P58692 (Asparagine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=asnS PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.2e-157
Identity = 279/459 (60.78%), Postives = 342/459 (74.51%), Query Frame = 0

Query: 109 QTLVVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQ 168
           ++LVV+GWVRT R      F+EVNDGS + N+Q V++     Y  +   L  TGASV V 
Sbjct: 17  ESLVVQGWVRTKRELKGFAFIEVNDGSSLGNLQVVINQDLPDYAVIVKQL-NTGASVEVN 76

Query: 169 GVVVASQGSKQKVELKLQKVVLVGKSDP-SYPVQKKRVSREFLRTKAHLRPRTNTFGAVA 228
           GV+VASQG  Q++ELK + V + G++DP +YP+QKKR S EFLRT  HLR RTN+FGAV 
Sbjct: 77  GVLVASQGKGQRIELKAEAVKVYGEADPETYPLQKKRHSFEFLRTIGHLRSRTNSFGAVF 136

Query: 229 RVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIP 288
           RVRNA + A H+FFQE GF+WV +PIITASDCEGAGE F VT+L          D+  IP
Sbjct: 137 RVRNACSAAIHQFFQERGFLWVHTPIITASDCEGAGELFSVTSL----------DLKQIP 196

Query: 289 KTNEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFW 348
           +T    ID+SQDFF KP +LTVSGQL AE  A A S+VYTFGPTFRAENSNTSRHLAEFW
Sbjct: 197 RTENQGIDYSQDFFAKPTYLTVSGQLEAEVMAMAFSNVYTFGPTFRAENSNTSRHLAEFW 256

Query: 349 MIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDF 408
           M+EPE+AF DL+ DM  A A+L+++  H+LE C ED++FFN  I+  ++    N++   F
Sbjct: 257 MVEPEMAFCDLEGDMDLAEAFLKHIFNHVLEKCPEDMEFFNQRIDNTVLATAENIINNQF 316

Query: 409 VQLTYTDAIELLLRAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFY 468
            +LTYTDAI+LL +A  KFEYPV WG DLQSEHERY+ E+ F   PVI+ DYP +IKAFY
Sbjct: 317 ERLTYTDAIKLLEKADVKFEYPVSWGLDLQSEHERYLAEQLFKK-PVIVTDYPAQIKAFY 376

Query: 469 MRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYG 528
           MR +DD KTV AMD+L P+IGE+IGGSQREERLD LE R+    +  +  WWYLDLRRYG
Sbjct: 377 MRLSDDEKTVRAMDVLAPKIGEIIGGSQREERLDVLERRVLAQGMQPEDLWWYLDLRRYG 436

Query: 529 SVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           +VPHAGFGLGFERLVQF+TG+ NIRD IPFPR+P +AEF
Sbjct: 437 TVPHAGFGLGFERLVQFITGMGNIRDVIPFPRTPQNAEF 463

BLAST of Lag0017484 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 553.9 bits (1426), Expect = 2.1e-156
Identity = 276/457 (60.39%), Postives = 345/457 (75.49%), Query Frame = 0

Query: 111 LVVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGV 170
           ++V+GW+RT R     +F+EVNDGS ++N+Q V+D +   YD++ S L  TGA++ V+G 
Sbjct: 19  VMVQGWLRTKRTLKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQL-QTGAALVVEGK 78

Query: 171 VVASQGSKQKVELKLQKVVLVGKSDP-SYPVQKKRVSREFLRTKAHLRPRTNTFGAVARV 230
           +  S G  Q+VELK  K+ L+G +DP SYP+QKKR S EFLRT  HLRPRTNT GAV RV
Sbjct: 79  LAPSPGKGQRVELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRV 138

Query: 231 RNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKT 290
           RNA A A H+FFQE GF+WV +PIITASDCEGAG+ F VTTL          D+  +PK 
Sbjct: 139 RNACATAIHQFFQERGFLWVHTPIITASDCEGAGDLFKVTTL----------DLQRVPKN 198

Query: 291 NEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMI 350
            EG ID+SQDFFGK A+LTVSGQL AE  A A  +VYTFGPTFRAENSNTSRHLAEFWM+
Sbjct: 199 GEG-IDYSQDFFGKQAYLTVSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMV 258

Query: 351 EPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQ 410
           EPE+AF DL+ D   A  +L+Y+ + +LE C ED++FF+  I+  ++    N++  +F  
Sbjct: 259 EPEMAFCDLEGDRQWAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAW 318

Query: 411 LTYTDAIELLLRAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMR 470
           LTY++AI+LL +A +KFEYPV WG DLQSEHERY+ E  F   P I+ DYPK+IKAFYMR
Sbjct: 319 LTYSEAIKLLEKADQKFEYPVAWGVDLQSEHERYLAETVFKR-PTIVTDYPKDIKAFYMR 378

Query: 471 QNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSV 530
            NDDGKTVAAMD+L P+IGE+IGGSQREERLD L  R+ +  + ++  WWYLDLRRYGSV
Sbjct: 379 LNDDGKTVAAMDILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSV 438

Query: 531 PHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           PHAGFGLGFER+VQF+TG+ NIRD IPFPR+P +AEF
Sbjct: 439 PHAGFGLGFERIVQFMTGMANIRDVIPFPRTPMNAEF 462

BLAST of Lag0017484 vs. ExPASy Swiss-Prot
Match: A3DBI2 (Asparagine--tRNA ligase OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=asnS PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.4e-155
Identity = 267/462 (57.79%), Postives = 343/462 (74.24%), Query Frame = 0

Query: 105 DRVGQTLVVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGAS 164
           D VG+ +VV GWVRT+R   +  F+E+NDGS   N+Q V +      +  E   ++ G++
Sbjct: 15  DYVGKEIVVAGWVRTVRDSKAFGFIELNDGSFFKNLQIVFEEKNLP-NFKEIAKLSVGSA 74

Query: 165 VWVQGVVVASQGSKQKVELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFG 224
           +   G +V + G+KQ  ELK  K+ + G S P YP+QKKR S E+LRT AHLRPRTNTF 
Sbjct: 75  IIAHGELVETPGAKQPFELKATKIEIEGASTPDYPLQKKRHSFEYLRTIAHLRPRTNTFS 134

Query: 225 AVARVRNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVD 284
           AV RVR+ LAYA HKFFQE GFV+V +PIIT SD EGAG+ F VTTL          D++
Sbjct: 135 AVFRVRSLLAYAIHKFFQERGFVYVHTPIITGSDAEGAGQMFKVTTL----------DIN 194

Query: 285 SIPKTNEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLA 344
           + P+  +G +D+S+DFF +   LTVSGQL  ETY+ A  ++YTFGPTFRAENSNT+RH A
Sbjct: 195 NPPRKEDGTVDFSKDFFDRETNLTVSGQLEGETYSMAFRNIYTFGPTFRAENSNTARHAA 254

Query: 345 EFWMIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVE 404
           EFWM+EPE+AFADL+DDM  A   L+Y++ + LEN  E+++FFN +I+  ++DRL N+V 
Sbjct: 255 EFWMVEPEIAFADLEDDMELAEDMLKYIINYCLENAPEEMEFFNNFIDNTLLDRLNNIVS 314

Query: 405 KDFVQLTYTDAIELLLRAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIK 464
            DF  +TYT+AI++L +A +KFEYPVKWG+DLQ+EHERY+TE+ F   PV + DYPK+IK
Sbjct: 315 SDFAHVTYTEAIDILSKADQKFEYPVKWGNDLQTEHERYLTEKVFKK-PVFVTDYPKDIK 374

Query: 465 AFYMRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLR 524
           AFYMR NDD KTVAAMD+LVP +GE+IGGSQREERLDYLE R+ +L L  + YWWYLDLR
Sbjct: 375 AFYMRLNDDNKTVAAMDLLVPGVGEIIGGSQREERLDYLENRMKELGLKMEDYWWYLDLR 434

Query: 525 RYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           RYGS  HAGFGLGFER + ++TG+ NIRD IPFPR+  SAEF
Sbjct: 435 RYGSTRHAGFGLGFERAIMYITGMSNIRDVIPFPRTVHSAEF 464

BLAST of Lag0017484 vs. ExPASy TrEMBL
Match: A0A6J1L1Y2 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111498374 PE=3 SV=1)

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 538/566 (95.05%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPA SLRFNPYS LRFI  FPKKLP  ILFPPIPRRHSSD FRRSF TAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPYILFPPIPRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLRISEVKGGLDEGLDRVGQTL+VKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEA NSDV SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVEKDFVQLTYTDA++LLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVKLLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFE+PVKWGSDLQSEHERY+TEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Lag0017484 vs. ExPASy TrEMBL
Match: A0A6J1G9C9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111451973 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 538/566 (95.05%), Postives = 546/566 (96.47%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPA SLRFNPYS LRFI  FPKKLP  ILFPPI RRHSSD FRRSF TAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPHILFPPISRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLRISEVKGGLDEGLDRVGQTL+VKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEA NSDV SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVEKDFVQLTYTDA+ELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFE+PVKWGSDLQSEHERY+TEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Lag0017484 vs. ExPASy TrEMBL
Match: A0A6J1DJ47 (Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111021381 PE=3 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 533/566 (94.17%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAAAMSPA SLRFNPYSTLRFIAGFP+KLPQ I  PPI RRHSS SFRRSF  AVSAALS
Sbjct: 1   MAAAMSPAISLRFNPYSTLRFIAGFPRKLPQSIPLPPITRRHSSGSFRRSF-RAVSAALS 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
           SGEA EKTKPEVS+DWKV RGEKVGEFRKKLR+SE+KGGLDEGLDRVGQTLVVKGWVRTL
Sbjct: 61  SGEAVEKTKPEVSKDWKVTRGEKVGEFRKKLRVSEIKGGLDEGLDRVGQTLVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           R QSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLI TGASVWV GVVVASQGSKQK
Sbjct: 121 RFQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLITTGASVWVHGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VG+SDPSYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF
Sbjct: 181 VELKLQKVVMVGESDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVW+SSPIITASDCEGAGEQFCVTTLIP SGEA NSDV +IPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWISSPIITASDCEGAGEQFCVTTLIPGSGEAANSDVGAIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DMACATAYLQYVVRH+LENCKEDLDFFNTWIEKG+IDRL NLVEKDFVQLTYTDAIELLL
Sbjct: 361 DMACATAYLQYVVRHVLENCKEDLDFFNTWIEKGVIDRLNNLVEKDFVQLTYTDAIELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFE+PVKWGSDLQSEHERY+TEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDS+WWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSFWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 565

BLAST of Lag0017484 vs. ExPASy TrEMBL
Match: A0A1S3BLJ5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103490884 PE=3 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 3.1e-304
Identity = 519/566 (91.70%), Postives = 538/566 (95.05%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAA +SPATSLRFNP STLRFI G P+KL   ILFPPI RRHSS  FRRSFC+AV A   
Sbjct: 1   MAAVISPATSLRFNPCSTLRFITGLPRKLSHHILFPPISRRHSSYYFRRSFCSAVPA--- 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
            GEAAEKTKP+VS+DWKV RGEKVGEFRKKLRISE+KGG DEGLDR+GQT VVKGWVRTL
Sbjct: 61  -GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLI TGASVWV+GVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKVV+VGKSDPSYPVQKK+VSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGE  +SDV SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DM+CATAYLQYVVRH+LENCKEDLDFFNTWIEKGIIDRLTNLVE DFVQL YTDAIELLL
Sbjct: 361 DMSCATAYLQYVVRHVLENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFEYPVKWGSDLQSEHERY+TE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVP+IGELIGGSQREERLDYLEARLDDLKLNK+SYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 562

BLAST of Lag0017484 vs. ExPASy TrEMBL
Match: A0A0A0L5I1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G020020 PE=3 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 2.0e-303
Identity = 518/566 (91.52%), Postives = 539/566 (95.23%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRRHSSDSFRRSFCTAVSAALS 60
           MAA +SPATSLRFNP STLRFI+G P+KLP  ILFPPI  RHSS  FRRSFC+AVSA   
Sbjct: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSA--- 60

Query: 61  SGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTL 120
            GEAAEKTKP+VS+DWKV RGEKVGEFRKKLRISE+KGG DEG+DR+GQT VV GWVRTL
Sbjct: 61  -GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGVDRLGQTFVVMGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLI TGASVWV+GVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQK 180

Query: 181 VELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240
           VELKLQKV++VGKSDPS+PVQKK+VSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF
Sbjct: 181 VELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGE   SDV SIPKT EGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPARSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLL 420
           DM+CATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVE DFVQL YTDAIELLL
Sbjct: 361 DMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLL 420

Query: 421 RAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RA KKFEYPVKWGSDLQSEHERY+TE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVP+IGELIGGSQREERLDYLEARLDDLKLNK+SYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 567
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 562

BLAST of Lag0017484 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 894.0 bits (2309), Expect = 6.1e-260
Identity = 443/573 (77.31%), Postives = 497/573 (86.74%), Query Frame = 0

Query: 1   MAAAMSPATSLRFNPYSTLRFIAGFPKKLPQRILFPPIPRR-------HSSDSFRRSFCT 60
           MAA   PATSLR    STLRF++ F    P   LF P+ RR         ++S RR FCT
Sbjct: 1   MAATFLPATSLRLTQNSTLRFLSFFTISNPSYSLFRPLRRRVLPPFDAFPANSRRRCFCT 60

Query: 61  AVSAALSSGEAAEKTKPEVSRDWKVARGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLVV 120
           AVS +L SG+  +         ++   G KVGEFRKKLRI+EVKGG DEGL RVGQ+L +
Sbjct: 61  AVSESLGSGDGNK------VESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNI 120

Query: 121 KGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVA 180
            GWVRTLR QSSVTF+E+NDGSC+SN+QCVM S  EGYDQVESG I TGASV VQG +VA
Sbjct: 121 MGWVRTLRSQSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVA 180

Query: 181 SQGSKQKVELKLQKVVLVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNAL 240
           SQG+KQKVELK++K+++VG+ D SYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRN L
Sbjct: 181 SQGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTL 240

Query: 241 AYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGF 300
           AYATHKFFQE+GFVWV+SPIITASDCEGAGEQFCVTTLIPSS E T++ +D+IPKT  G 
Sbjct: 241 AYATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHENTDTSIDAIPKTKGGL 300

Query: 301 IDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360
           IDWSQDFFGKPAFLTVSGQLN ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL
Sbjct: 301 IDWSQDFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360

Query: 361 AFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYT 420
           AFADLDDDMACATAYLQYVV+++L+NCKED++FF+TWIEKGII RL+++ EK+F+QL YT
Sbjct: 361 AFADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYT 420

Query: 421 DAIELLLRAKKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDD 480
           DAIE+LL+A KKF++PVKWG DLQSEHERY+TEEAFGG PVIIRDYPKEIKAFYMR+NDD
Sbjct: 421 DAIEILLKANKKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDD 480

Query: 481 GKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAG 540
           GKTVAAMDMLVPRIGELIGGSQREERL+ LEARLD+LKLNK+SYWWYLDLRRYGSVPHAG
Sbjct: 481 GKTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAG 540

Query: 541 FGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           FGLGFERLVQFVTGIDNIRD IPFPR+P SAEF
Sbjct: 541 FGLGFERLVQFVTGIDNIRDVIPFPRTPASAEF 567

BLAST of Lag0017484 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 499.2 bits (1284), Expect = 4.3e-141
Identity = 269/550 (48.91%), Postives = 351/550 (63.82%), Query Frame = 0

Query: 86  EFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTLRVQSSVT--FMEVNDGSCISNMQCV 145
           +F  ++ I  + GG   G    GQ + + GWV+T R Q   T  F+EVNDGSC +N+Q +
Sbjct: 28  QFSDRVLIRSILGG---GAKLAGQKVRIGGWVKTGRQQGKGTFAFLEVNDGSCPANLQVM 87

Query: 146 MDSTTEGYDQVESGLIATGASVWVQGVV---VASQGSKQKVELKLQKVVLVGKSDP-SYP 205
           +DS+   YD   S L+ATG  V V GV+      +G KQ +EL ++ V+ VG  DP +YP
Sbjct: 88  VDSSL--YDL--SRLVATGTCVTVDGVLKIPPEGKGLKQSIELSVETVIAVGTVDPTTYP 147

Query: 206 VQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDC 265
           + K +++ EFLR   HLR RTN   AVAR+RNALA+ATH FFQE+ F+++ +PIIT SDC
Sbjct: 148 LPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 207

Query: 266 EGAGEQFCVTTLIPSSGEATNSDVDSIPKT------------------------------ 325
           EGAGE F VTTLI  +       +D+ P T                              
Sbjct: 208 EGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEE 267

Query: 326 ------------------------------NEGFIDWSQDFFGKPAFLTVSGQLNAETYA 385
                                         N+G ID+S DFFG+ AFLTVSGQL  ETYA
Sbjct: 268 ITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVETYA 327

Query: 386 TALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILEN 445
            ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFAD+ DDM CA AY++Y+ + +++ 
Sbjct: 328 CALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDK 387

Query: 446 CKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLLRA----KKKFEYPVKWGSD 505
           C +D++  +  +++G   RL  + +  F ++TYT+AIE L +A    K  F+  V+WG D
Sbjct: 388 CGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEWGID 447

Query: 506 LQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQ 565
           L SEHERY+TE  F   P+I+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIGGSQ
Sbjct: 448 LASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 507

BLAST of Lag0017484 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 483.0 bits (1242), Expect = 3.2e-136
Identity = 267/549 (48.63%), Postives = 346/549 (63.02%), Query Frame = 0

Query: 86  EFRKKLRISEVKGGLDEGLDRVGQTLVVKGWVRTLRVQSSVT--FMEVNDGSCISNMQCV 145
           +F  ++ I  +    D G    GQT+ + GWV++ R Q   T  F+ VNDGSC +N+Q +
Sbjct: 28  QFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRDQGKRTFSFLAVNDGSCPANLQVM 87

Query: 146 MDSTTEGYDQVESGLIATGASVWVQGVVVA---SQGSKQKVELKLQKVVLVGKSDPS-YP 205
           +D +   YD   S L+ATG  V V GV+      +G++Q++EL + KV+ VG  D S YP
Sbjct: 88  VDPSL--YD--VSNLVATGTCVTVDGVLKVPPKGKGTQQQIELNVVKVIDVGTVDASKYP 147

Query: 206 VQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDC 265
           + K +++ E LR   HLR RTN+  AVAR+RNALA+ATH FFQE+ F+++ +PIIT SDC
Sbjct: 148 LPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 207

Query: 266 EGAGEQFCVTTLIPSSGEATNSDVDSIPKT------------------------------ 325
           EGAGE F  TTLI  +       +D+ P T                              
Sbjct: 208 EGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEA 267

Query: 326 ------------------------------NEGFIDWSQDFFGKPAFLTVSGQLNAETYA 385
                                          +G ID+S+DFFG+ AFLTVSGQL  ETYA
Sbjct: 268 ITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVETYA 327

Query: 386 TALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILEN 445
            ALS+VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL+DDM CA AY++Y+   +LE 
Sbjct: 328 CALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEK 387

Query: 446 CKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLLRA---KKKFEYPVKWGSDL 505
           C  D++      + G IDRL  +    F ++TYT AIELL  A    K+F+  V+WG DL
Sbjct: 388 CYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGIDL 447

Query: 506 QSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQR 565
            SEHERY+TE  F   P+I+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIGGSQR
Sbjct: 448 ASEHERYLTEVLFQK-PLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQR 507

BLAST of Lag0017484 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 339.7 bits (870), Expect = 4.4e-93
Identity = 193/503 (38.37%), Postives = 286/503 (56.86%), Query Frame = 0

Query: 126 VTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGV--VVASQGSKQKVEL 185
           V +  ++DGS IS++Q V+DS        +  L+A G  +  +GV  +  +  +K  +EL
Sbjct: 139 VFYFLISDGSSISSLQVVVDSALSTVPATQ--LMALGTCIVAEGVLRLPLAASAKHVIEL 198

Query: 186 KLQKVVLVGKSDP-SYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQ 245
           + +K++ VG  DP  YP+ KK++    LR  +H RPRT T G+V RV +AL  A+H F Q
Sbjct: 199 EAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQ 258

Query: 246 ENGFVWVSSPIITASDCEGAGEQFCVTTLI------------------------------ 305
            +GF +V  P+IT +   G GE F VTTL+                              
Sbjct: 259 YHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKE 318

Query: 306 -----------PSSGEATNSDVDSIPKTNE-------------------GFIDWSQDFFG 365
                       S+ E   + V  + KTN+                     +D+S+DFFG
Sbjct: 319 KTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFG 378

Query: 366 KPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDM 425
           +  +LT SG+ + E+YA+AL  VYTFGP F A+  + +RHLAE W +E E+AFA+LDD M
Sbjct: 379 RDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAM 438

Query: 426 ACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLLRA 485
            CA  Y +++ +++LEN  ED+ F +  ++K I  RL        ++ +YT+ I LL +A
Sbjct: 439 DCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKA 498

Query: 486 -KKKFEYPVKWGSDLQSEHERYVTEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMD 545
              KFE   +WG  L +EH  Y+T+E + G PVI+  YPK IK FY+R NDD KTVAA D
Sbjct: 499 TTTKFETKPEWGVALTTEHLSYLTDEIYKG-PVIVHTYPKAIKQFYVRLNDDKKTVAAFD 558

Query: 546 MLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFERL 565
           ++VP++G +I GSQ EER + L+AR+ +    ++ + WYLDLRR+G+V H+G  L  E++
Sbjct: 559 LVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQM 618

BLAST of Lag0017484 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.6e-26
Identity = 116/485 (23.92%), Postives = 211/485 (43.51%), Query Frame = 0

Query: 107 VGQTLVVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVW 166
           VG  + ++G +   R+  +  F+ + +      +QCV++ T  G + ++     +  SV 
Sbjct: 84  VGSEVSIRGRLHKNRLVGTKLFVILRESG--FTVQCVVEETRVGANMIKFVKQLSRESV- 143

Query: 167 VQGVVVASQ------GSKQKVELKLQKVVLVGKSDPSYPV-------------------- 226
           V+ + V S       G+ Q+VE+ ++K+  + +S P+ P+                    
Sbjct: 144 VELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLVVEDAARSESDIEKSGKDGK 203

Query: 227 QKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVSSPIITASDCE 286
           Q  RV ++       L  RT    A+ R++  +  A  ++ Q  GF+ + +P + A   E
Sbjct: 204 QAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYLQSKGFLEIHTPKLIAGSSE 263

Query: 287 GAGEQFCVTTLIPSSGEATNSDVDSIPKTNEGFIDWSQDFFGKPAFLTVSGQLNAE-TYA 346
           G    F +                              D+ G+PA L  S QL+ +    
Sbjct: 264 GGSAVFRL------------------------------DYKGQPACLAQSPQLHKQMAIC 323

Query: 347 TALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAF-ADLDDDMACATAYLQYVVRHILE 406
             +  V+  GP FRAE+S T RHL EF  ++ E+       + M        ++   I E
Sbjct: 324 GDMRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEE 383

Query: 407 NCKEDLDFFNTWIEKGIIDRLTNLVEKDFVQLTYTDAIELLLRAKKKFEYPVKWGSDLQS 466
            C ++L+          +  L        ++LT+ + I++L  A ++ + P+    DL +
Sbjct: 384 RCPKELESVRKQYPFQSLKFLPQT-----LRLTFAEGIQMLKEAGEEVD-PL---GDLNT 443

Query: 467 EHERYVTE---EAFGGCPVIIRDYPKEIKAFY-MRQNDDGKTVAAMDMLVPRIGELIGGS 526
           E ER + +   E +     ++  YP  ++ FY M   +D     + D+ + R  E++ G+
Sbjct: 444 ESERKLGQLVLEKYKTEFYMLHRYPSAVRPFYTMPYENDSNYSNSFDVFI-RGEEIMSGA 503

Query: 527 QREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDA 560
           QR    + LE R  +  ++  +   Y+D  RYG+ PH GFG+G ER+V  +  ++NIR  
Sbjct: 504 QRIHDPELLEKRARECGIDVKTISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKT 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023005353.10.0e+0095.05asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima][more]
XP_022948254.10.0e+0095.05asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] >KAG65... [more]
XP_022154023.10.0e+0094.17asparagine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia][more]
XP_023540678.10.0e+0094.88asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo... [more]
XP_038904123.13.0e-30993.29asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispi... [more]
Match NameE-valueIdentityDescription
O485938.5e-25977.31Asparagine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q6MEC91.6e-16458.37Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
P586927.2e-15760.78Asparagine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
P522762.1e-15660.39Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
A3DBI21.4e-15557.79Asparagine--tRNA ligase OS=Hungateiclostridium thermocellum (strain ATCC 27405 /... [more]
Match NameE-valueIdentityDescription
A0A6J1L1Y20.0e+0095.05Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111498374 PE=3 SV=1[more]
A0A6J1G9C90.0e+0095.05Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111451973 PE=3 SV=1[more]
A0A6J1DJ470.0e+0094.17Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111021381 PE=3 SV=1[more]
A0A1S3BLJ53.1e-30491.70Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103490884 PE=3 SV=1[more]
A0A0A0L5I12.0e-30391.52Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G020020 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G17300.16.1e-26077.31Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.14.3e-14148.91Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT5G56680.13.2e-13648.63Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT3G07420.14.4e-9338.37asparaginyl-tRNA synthetase 2 [more]
AT4G26870.11.6e-2623.92Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 481..497
score: 45.05
coord: 307..319
score: 41.78
coord: 521..535
score: 54.7
coord: 323..336
score: 44.32
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 199..564
e-value: 2.7E-106
score: 357.7
NoneNo IPR availableGENE3D2.40.50.140coord: 88..196
e-value: 4.3E-16
score: 60.7
NoneNo IPR availablePANTHERPTHR22594:SF46ASPARAGINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIALcoord: 36..566
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 36..566
NoneNo IPR availableCDDcd00776AsxRS_corecoord: 204..562
e-value: 2.7648E-153
score: 440.464
NoneNo IPR availableCDDcd04318EcAsnRS_like_Ncoord: 110..193
e-value: 1.0412E-30
score: 112.659
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 207..561
IPR004522Asparagine-tRNA ligaseTIGRFAMTIGR00457TIGR00457coord: 100..566
e-value: 1.1E-187
score: 622.4
IPR004522Asparagine-tRNA ligaseHAMAPMF_00534Asn_tRNA_synthcoord: 96..566
score: 44.5844
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 207..560
e-value: 3.1E-79
score: 266.3
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 228..556
score: 16.299562
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 105..195

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017484.1Lag0017484.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding