Lag0017463 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017463
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	ethylene-responsive transcription factor ERF039-like
Location	chr5: 3982460 .. 3983146 (+)
RNA-Seq Expression	Lag0017463
Synteny	Lag0017463

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGATCCTCTTCACAGATTCCAATTTCACTCTCAATCTTCTTCATCATCTTCTTCTTCATCTTCTTCTTCAGATATCAAAAATTCCATCACAGAGAAAAACTCCAAAAAGCTCAAACTCGTCAACGACGGCAAACATCCCACCTATCGCGGCGTCCGAATGCGACAATGGGGGAAATGGGTATCGGAAATCAGAGAACCCAAGAAGAAATCAAGAATTTGGTTAGGCACATTCTCCACGCCGGAAATGGCCGCCAGAGCCCACGACGTCGCCGCCAGAACCATCAAAGGCCACTCCGCCTACCTCAACTTCCCGGAATTGGCTCACCGCCTCCCCATTCCGGCCAGCTCTTCGCCCAAGGACATTCGAGCTGCTGCCGCCAAAGCGGCCTCGTTCAGCAATAATCTTCTCCTCGATCCGACCGACGACGACGAAGAAGACGGCGCCGCTCAATCGGACCTCAATCGCTCTCCTTCCTCCTCCTCCGCCGCCGCCGCGAAATCGCCCGGTGAAATCGACGATCCTTTCTTCGATCTCCCCGATCTGTTCCTTCATCCCAATCACCAAATGGATGCATTCTGTTTCTCGCAAATTCTTCCGATCGATGGCTTCGACTCTGTCTTCGGCCGGCCACCATCGCCGTCGCCGTCGGAATATTTCGCCTATAGAGGTAGCCTACTCTAG

mRNA sequence

ATGGAAGATCCTCTTCACAGATTCCAATTTCACTCTCAATCTTCTTCATCATCTTCTTCTTCATCTTCTTCTTCAGATATCAAAAATTCCATCACAGAGAAAAACTCCAAAAAGCTCAAACTCGTCAACGACGGCAAACATCCCACCTATCGCGGCGTCCGAATGCGACAATGGGGGAAATGGGTATCGGAAATCAGAGAACCCAAGAAGAAATCAAGAATTTGGTTAGGCACATTCTCCACGCCGGAAATGGCCGCCAGAGCCCACGACGTCGCCGCCAGAACCATCAAAGGCCACTCCGCCTACCTCAACTTCCCGGAATTGGCTCACCGCCTCCCCATTCCGGCCAGCTCTTCGCCCAAGGACATTCGAGCTGCTGCCGCCAAAGCGGCCTCGTTCAGCAATAATCTTCTCCTCGATCCGACCGACGACGACGAAGAAGACGGCGCCGCTCAATCGGACCTCAATCGCTCTCCTTCCTCCTCCTCCGCCGCCGCCGCGAAATCGCCCGGTGAAATCGACGATCCTTTCTTCGATCTCCCCGATCTGTTCCTTCATCCCAATCACCAAATGGATGCATTCTGTTTCTCGCAAATTCTTCCGATCGATGGCTTCGACTCTGTCTTCGGCCGGCCACCATCGCCGTCGCCGTCGGAATATTTCGCCTATAGAGGTAGCCTACTCTAG

Coding sequence (CDS)

ATGGAAGATCCTCTTCACAGATTCCAATTTCACTCTCAATCTTCTTCATCATCTTCTTCTTCATCTTCTTCTTCAGATATCAAAAATTCCATCACAGAGAAAAACTCCAAAAAGCTCAAACTCGTCAACGACGGCAAACATCCCACCTATCGCGGCGTCCGAATGCGACAATGGGGGAAATGGGTATCGGAAATCAGAGAACCCAAGAAGAAATCAAGAATTTGGTTAGGCACATTCTCCACGCCGGAAATGGCCGCCAGAGCCCACGACGTCGCCGCCAGAACCATCAAAGGCCACTCCGCCTACCTCAACTTCCCGGAATTGGCTCACCGCCTCCCCATTCCGGCCAGCTCTTCGCCCAAGGACATTCGAGCTGCTGCCGCCAAAGCGGCCTCGTTCAGCAATAATCTTCTCCTCGATCCGACCGACGACGACGAAGAAGACGGCGCCGCTCAATCGGACCTCAATCGCTCTCCTTCCTCCTCCTCCGCCGCCGCCGCGAAATCGCCCGGTGAAATCGACGATCCTTTCTTCGATCTCCCCGATCTGTTCCTTCATCCCAATCACCAAATGGATGCATTCTGTTTCTCGCAAATTCTTCCGATCGATGGCTTCGACTCTGTCTTCGGCCGGCCACCATCGCCGTCGCCGTCGGAATATTTCGCCTATAGAGGTAGCCTACTCTAG

Protein sequence

MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSPKDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDLPDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFAYRGSLL

Homology

[Close]

BLAST of Lag0017463 vs. NCBI nr
Match: XP_038905215.1 (ethylene-responsive transcription factor ERF039-like [Benincasa hispida])

HSP 1 Score: 365.5 bits (937), Expect = 3.2e-97
Identity = 196/229 (85.59%), Postives = 205/229 (89.52%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSI E+NSKKLKLVNDG HPTYRGVRMRQWGK
Sbjct: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSIKERNSKKLKLVNDGTHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLP PASSSP
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPRPASSSP 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDL 180
           KDIRAAAAKAASF+N  L     DDE + AA+SDLN + S SSA AAKS G+IDDPFFDL
Sbjct: 121 KDIRAAAAKAASFTNLNL----HDDEHNAAAKSDLNLNRSPSSAVAAKSSGDIDDPFFDL 180

Query: 181 PDLFL-HPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFAYRGSLL 229
           PDLFL  PNHQ+D FCFSQ LPIDGFDSVF RP  PSPS+YFAYRGS+L
Sbjct: 181 PDLFLDDPNHQIDTFCFSQFLPIDGFDSVFTRP--PSPSDYFAYRGSIL 223

[Close]

BLAST of Lag0017463 vs. NCBI nr
Match: XP_022965173.1 (ethylene-responsive transcription factor ERF039-like [Cucurbita maxima])

HSP 1 Score: 333.2 bits (853), Expect = 1.8e-87
Identity = 183/238 (76.89%), Postives = 191/238 (80.25%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPLHR Q    SSSSSSSSSSSSDIKN + EK SKK+KL NDGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLHRVQ---SSSSSSSSSSSSSDIKNGVKEKISKKVKLGNDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAH LP PA+SSP
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHCLPRPATSSP 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNR----------SPSSSSAAAAKSP 180
            DIRAAAAKAASF+        DD     AAQSDLN           S SSSS+ AAKSP
Sbjct: 121 NDIRAAAAKAASFT-------IDDHHHIAAAQSDLNHNRSTSSSSSSSSSSSSSVAAKSP 180

Query: 181 GEIDDPFFDLPDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFAYRGSLL 229
           GEIDDPFFDLPDLFL PNHQ+  FC+SQ LPI+ FDSV   PPSP PSEYFAYRGSLL
Sbjct: 181 GEIDDPFFDLPDLFLDPNHQIGTFCYSQFLPIESFDSVLAPPPSPPPSEYFAYRGSLL 228

[Close]

BLAST of Lag0017463 vs. NCBI nr
Match: XP_022923459.1 (ethylene-responsive transcription factor ERF039-like [Cucurbita moschata])

HSP 1 Score: 331.6 bits (849), Expect = 5.2e-87
Identity = 182/230 (79.13%), Postives = 191/230 (83.04%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPLHR Q     SSSSSSSSSSSDIKN + EK SKK+KL NDGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLHRLQ-----SSSSSSSSSSSDIKNGVKEKISKKVKLGNDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAH LP PA+SSP
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHCLPRPATSSP 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEE-DGAAQSDLNRSPS-SSSAAAAKSPGEIDDPFF 180
            DIRAAAAKAASF+       TDD      AAQSDLN + S SSS+ AAKSPGEIDDPFF
Sbjct: 121 NDIRAAAAKAASFT-------TDDHHHIIAAAQSDLNHNRSTSSSSVAAKSPGEIDDPFF 180

Query: 181 DLPDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFAYRGSLL 229
           DLPDLFL PNHQ+  FC+SQ LPI+ FDSV   PPSP PSEYFAYRGSLL
Sbjct: 181 DLPDLFLDPNHQIGTFCYSQFLPIESFDSVLAPPPSPPPSEYFAYRGSLL 218

[Close]

BLAST of Lag0017463 vs. NCBI nr
Match: XP_023552457.1 (ethylene-responsive transcription factor ERF039-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.9 bits (842), Expect = 3.4e-86
Identity = 181/238 (76.05%), Postives = 191/238 (80.25%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPLHR Q    SSSSSSSSSSSSDIKN + EK SKK+KL  DGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLHRLQ---SSSSSSSSSSSSSDIKNGVKEKISKKVKLGTDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAH LP PA+SSP
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHCLPRPATSSP 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNR----------SPSSSSAAAAKSP 180
            DIRAAAAKAASF+ +      D +    AAQSDLN           S SSSS+ AAKSP
Sbjct: 121 NDIRAAAAKAASFTTH------DHNHIIAAAQSDLNHNRSTSSSSSSSSSSSSSVAAKSP 180

Query: 181 GEIDDPFFDLPDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFAYRGSLL 229
           GEIDDPFFDLPDLFL PNHQ+  FC+SQ LPI+ FDSV   PPSP PSEYFAYRGSLL
Sbjct: 181 GEIDDPFFDLPDLFLDPNHQIGTFCYSQFLPIESFDSVLAPPPSPPPSEYFAYRGSLL 229

[Close]

BLAST of Lag0017463 vs. NCBI nr
Match: XP_022945031.1 (ethylene-responsive transcription factor ERF038-like [Cucurbita moschata] >KAG6596868.1 Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028400.1 Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 328.2 bits (840), Expect = 5.7e-86
Identity = 176/222 (79.28%), Postives = 187/222 (84.23%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPL RFQF           SSSSD+K    EKNSKK+KL NDGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLQRFQF----------QSSSSDMK----EKNSKKMKLGNDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHR P PASSS 
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRFPRPASSSA 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDL 180
           KDIRAAAAKAA+F     ++ TD+D++D A+QSD NRSP SS AAAAKSPGEIDDPFFDL
Sbjct: 121 KDIRAAAAKAATFP----INSTDEDDDDAASQSDRNRSP-SSVAAAAKSPGEIDDPFFDL 180

Query: 181 PDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFA 223
           PDLFLHPNHQ+  FCFSQ+LPIDGFDS FGRP  PSPSEYFA
Sbjct: 181 PDLFLHPNHQIGTFCFSQMLPIDGFDSAFGRP--PSPSEYFA 201

[Close]

BLAST of Lag0017463 vs. ExPASy Swiss-Prot
Match: Q9ZQP3 (Ethylene-responsive transcription factor ERF038 OS=Arabidopsis thaliana OX=3702 GN=ERF038 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.2e-39
Identity = 93/149 (62.42%), Postives = 104/149 (69.80%), Query Frame = 0


Query: 46  KHPTYRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNF 105
           KHP +RGVRMRQWGKWVSEIREPKKKSRIWLGTFST EMAARAHDVAA  IKG SA+LNF
Sbjct: 41  KHPNFRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTAEMAARAHDVAALAIKGGSAHLNF 100

Query: 106 PELAHRLPIPASSSPKDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAA 165
           PELA+ LP PAS+ PKDI+AAAA AA+          D D E  +       + +SS A 
Sbjct: 101 PELAYHLPRPASADPKDIQAAAAAAAA------AVAIDMDVETSSPSPSPTVTETSSPAM 160

Query: 166 AAKSPGEIDDPFFDLPDLFLHPNHQMDAF 195
            A S    DD F DLPDL L+ NH +D F
Sbjct: 161 IALS----DDAFSDLPDLLLNVNHNIDGF 179

[Close]

BLAST of Lag0017463 vs. ExPASy Swiss-Prot
Match: Q9SUK8 (Ethylene-responsive transcription factor ERF039 OS=Arabidopsis thaliana OX=3702 GN=ERF039 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 3.0e-37
Identity = 94/175 (53.71%), Postives = 110/175 (62.86%), Query Frame = 0


Query: 18  SSSSSSSSDIKNSITEKNSKKLKLVND----GKHPTYRGVRMRQWGKWVSEIREPKKKSR 77
           SSS  S  ++++ + EK  K  K  N+     K P +RGVRMRQWGKWVSEIREP+KKSR
Sbjct: 4   SSSHESQRNLRSPVPEKTGKSSKTKNEQKGVSKQPNFRGVRMRQWGKWVSEIREPRKKSR 63

Query: 78  IWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSPKDIRAAAAKAASF 137
           IWLGTFSTPEMAARAHDVAA  IKG SA+LNFPELA+ LP PAS+ PKDI+ AAA AA+ 
Sbjct: 64  IWLGTFSTPEMAARAHDVAALAIKGGSAHLNFPELAYHLPRPASADPKDIQEAAAAAAAV 123

Query: 138 SNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDLPDLFLHPN 189
                                  ++P S S+    SP   DD F DLPDL L  N
Sbjct: 124 D---------------------WKAPESPSSTVTSSP-VADDAFSDLPDLLLDVN 156

[Close]

BLAST of Lag0017463 vs. ExPASy Swiss-Prot
Match: Q8LBQ7 (Ethylene-responsive transcription factor ERF034 OS=Arabidopsis thaliana OX=3702 GN=ERF034 PE=2 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 1.6e-35
Identity = 110/248 (44.35%), Postives = 132/248 (53.23%), Query Frame = 0


Query: 14  SSSSSSSSSSSSDIKNSITEKNSK-------------------------KLKLVNDG-KH 73
           SS ++SSSSSSS   N I E NSK                         K +  N G KH
Sbjct: 38  SSPTTSSSSSSSTNSNFIEEDNSKRKASRRSLSSLVSVEDDDDQNGGGGKRRKTNGGDKH 97

Query: 74  PTYRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPE 133
           PTYRGVRMR WGKWVSEIREP+KKSRIWLGT+ T EMAARAHDVAA  IKG +AYLNFP+
Sbjct: 98  PTYRGVRMRSWGKWVSEIREPRKKSRIWLGTYPTAEMAARAHDVAALAIKGTTAYLNFPK 157

Query: 134 LAHRLPIPASSSPKDIRAAAAKAA--------SFSNNLLLDPTD--------------DD 193
           LA  LP P ++SPKDI+AAA+ AA          SN+ + +  +              D 
Sbjct: 158 LAGELPRPVTNSPKDIQAAASLAAVNWQDSVNDVSNSEVAEIVEAEPSRAVVAQLFSSDT 217

Query: 194 EEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDLPDLFLHPNHQM---DAFC-FSQILP 210
                 QS      S +S +A       ++  FDLPDLF   N  M   DAFC +S    
Sbjct: 218 STTTTTQSQEYSEASCASTSACTDKDSEEEKLFDLPDLFTDENEMMIRNDAFCYYSSTWQ 277

[Close]

BLAST of Lag0017463 vs. ExPASy Swiss-Prot
Match: Q9M210 (Ethylene-responsive transcription factor ERF035 OS=Arabidopsis thaliana OX=3702 GN=ERF035 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 8.2e-35
Identity = 102/204 (50.00%), Postives = 125/204 (61.27%), Query Frame = 0


Query: 12  SQSSSSSSSSSSSSDIKNSITEKNS-----KKLKLVNDGKHPT-YRGVRMRQWGKWVSEI 71
           S SS  +SSS S S  K S+ + N      +K  + +D K+PT YRGVRMR WGKWVSEI
Sbjct: 27  SSSSVVTSSSDSWSTSKRSLVQDNDSGGKRRKSNVSDDNKNPTSYRGVRMRSWGKWVSEI 86

Query: 72  REPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSPKDIRA 131
           REP+KKSRIWLGT+ T EMAARAHDVAA  IKG+S +LNFPEL+  LP P S SPKDI+A
Sbjct: 87  REPRKKSRIWLGTYPTAEMAARAHDVAALAIKGNSGFLNFPELSGLLPRPVSCSPKDIQA 146

Query: 132 AAAKAASFS-------NNLLLDPTDDDEEDGAAQSDLNRS---PSSSSAAAAKSPGEIDD 191
           AA KAA  +       +  L D     E    AQS  + S    S +S  ++      ++
Sbjct: 147 AATKAAEATTWHKPVIDKKLADELSHSELLSTAQSSTSSSFVFSSDTSETSSTDKESNEE 206

Query: 192 PFFDLPDLF----LHPNHQMDAFC 196
             FDLPDLF    ++PN   DAFC
Sbjct: 207 TVFDLPDLFTDGLMNPN---DAFC 227

[Close]

BLAST of Lag0017463 vs. ExPASy Swiss-Prot
Match: O80654 (Ethylene-responsive transcription factor ERF037 OS=Arabidopsis thaliana OX=3702 GN=ERF037 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 5.9e-33
Identity = 86/160 (53.75%), Postives = 102/160 (63.75%), Query Frame = 0


Query: 48  PTYRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPE 107
           P YRGVRMR WGKWVSEIREP+KKSRIWLGTFSTPEMAARAHD AA TIKG SA LNFPE
Sbjct: 41  PGYRGVRMRTWGKWVSEIREPRKKSRIWLGTFSTPEMAARAHDAAALTIKGTSAVLNFPE 100

Query: 108 LAHRLPIPASSSPKDIRAAAAKAA------SFSNNLLLDPTD-----DDEEDGAAQSDLN 167
           LA  LP PASSSP+D++AAAA AA      S S+ ++ DPT      + +   ++ S   
Sbjct: 101 LATYLPRPASSSPRDVQAAAAVAAAMDFSPSSSSLVVSDPTTVIAPAETQLSSSSYSTCT 160

Query: 168 RSPSSSSAAAAKSPGEIDDPFFDLPDLFLHPNHQMDAFCF 197
            S  S S+  A S  E      +LP L    +  +  F +
Sbjct: 161 SSSLSPSSEEAASTAEELSEIVELPSLETSYDESLSEFVY 200

[Close]

BLAST of Lag0017463 vs. ExPASy TrEMBL
Match: A0A6J1HN35 (ethylene-responsive transcription factor ERF039-like OS=Cucurbita maxima OX=3661 GN=LOC111465108 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 8.6e-88
Identity = 183/238 (76.89%), Postives = 191/238 (80.25%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPLHR Q    SSSSSSSSSSSSDIKN + EK SKK+KL NDGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLHRVQ---SSSSSSSSSSSSSDIKNGVKEKISKKVKLGNDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAH LP PA+SSP
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHCLPRPATSSP 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNR----------SPSSSSAAAAKSP 180
            DIRAAAAKAASF+        DD     AAQSDLN           S SSSS+ AAKSP
Sbjct: 121 NDIRAAAAKAASFT-------IDDHHHIAAAQSDLNHNRSTSSSSSSSSSSSSSVAAKSP 180

Query: 181 GEIDDPFFDLPDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFAYRGSLL 229
           GEIDDPFFDLPDLFL PNHQ+  FC+SQ LPI+ FDSV   PPSP PSEYFAYRGSLL
Sbjct: 181 GEIDDPFFDLPDLFLDPNHQIGTFCYSQFLPIESFDSVLAPPPSPPPSEYFAYRGSLL 228

[Close]

BLAST of Lag0017463 vs. ExPASy TrEMBL
Match: A0A6J1E6G7 (ethylene-responsive transcription factor ERF039-like OS=Cucurbita moschata OX=3662 GN=LOC111431149 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.5e-87
Identity = 182/230 (79.13%), Postives = 191/230 (83.04%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPLHR Q     SSSSSSSSSSSDIKN + EK SKK+KL NDGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLHRLQ-----SSSSSSSSSSSDIKNGVKEKISKKVKLGNDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAH LP PA+SSP
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHCLPRPATSSP 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEE-DGAAQSDLNRSPS-SSSAAAAKSPGEIDDPFF 180
            DIRAAAAKAASF+       TDD      AAQSDLN + S SSS+ AAKSPGEIDDPFF
Sbjct: 121 NDIRAAAAKAASFT-------TDDHHHIIAAAQSDLNHNRSTSSSSVAAKSPGEIDDPFF 180

Query: 181 DLPDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFAYRGSLL 229
           DLPDLFL PNHQ+  FC+SQ LPI+ FDSV   PPSP PSEYFAYRGSLL
Sbjct: 181 DLPDLFLDPNHQIGTFCYSQFLPIESFDSVLAPPPSPPPSEYFAYRGSLL 218

[Close]

BLAST of Lag0017463 vs. ExPASy TrEMBL
Match: A0A6J1FZQ3 (ethylene-responsive transcription factor ERF038-like OS=Cucurbita moschata OX=3662 GN=LOC111449391 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.8e-86
Identity = 176/222 (79.28%), Postives = 187/222 (84.23%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPL RFQF           SSSSD+K    EKNSKK+KL NDGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLQRFQF----------QSSSSDMK----EKNSKKMKLGNDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHR P PASSS 
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRFPRPASSSA 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDL 180
           KDIRAAAAKAA+F     ++ TD+D++D A+QSD NRSP SS AAAAKSPGEIDDPFFDL
Sbjct: 121 KDIRAAAAKAATFP----INSTDEDDDDAASQSDRNRSP-SSVAAAAKSPGEIDDPFFDL 180

Query: 181 PDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFA 223
           PDLFLHPNHQ+  FCFSQ+LPIDGFDS FGRP  PSPSEYFA
Sbjct: 181 PDLFLHPNHQIGTFCFSQMLPIDGFDSAFGRP--PSPSEYFA 201

[Close]

BLAST of Lag0017463 vs. ExPASy TrEMBL
Match: A0A6J1L2H3 (ethylene-responsive transcription factor ERF038-like OS=Cucurbita maxima OX=3661 GN=LOC111498502 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 6.2e-86
Identity = 177/222 (79.73%), Postives = 186/222 (83.78%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPL RFQF          SSSSSD+K    EKNSKK+KL NDGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLQRFQF---------QSSSSSDMK----EKNSKKMKLGNDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHR P PASSS 
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRFPRPASSSA 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDL 180
           KDIRAAAAKAA+F  N      DDD++D A+QSD NRSP SS AAAAKSPGEIDDPFFDL
Sbjct: 121 KDIRAAAAKAATFPIN--STDEDDDDDDAASQSDRNRSP-SSVAAAAKSPGEIDDPFFDL 180

Query: 181 PDLFLHPNHQMDAFCFSQILPIDGFDSVFGRPPSPSPSEYFA 223
           PDLFLHPNHQ+  FCFSQ+LPIDGFDS FGRP  PSPSEYF+
Sbjct: 181 PDLFLHPNHQIGTFCFSQMLPIDGFDSAFGRP--PSPSEYFS 204

[Close]

BLAST of Lag0017463 vs. ExPASy TrEMBL
Match: A0A5A7TD44 (Ethylene-responsive transcription factor ERF039 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold477G00450 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 5.2e-85
Identity = 176/230 (76.52%), Postives = 189/230 (82.17%), Query Frame = 0


Query: 1   MEDPLHRFQFHSQSSSSSSSSSSSSDIKNSITEKNSKKLKLVNDGKHPTYRGVRMRQWGK 60
           MEDPL RFQFHSQ      SSSSSSDIKN I EK SKK+KL +DGKHPTYRGVRMRQWGK
Sbjct: 1   MEDPLQRFQFHSQ------SSSSSSDIKNCIKEKTSKKVKLGSDGKHPTYRGVRMRQWGK 60

Query: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSP 120
           WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAA+TIKGHSAYLNFPELAHRLP PASSSP
Sbjct: 61  WVSEIREPKKKSRIWLGTFSTPEMAARAHDVAAKTIKGHSAYLNFPELAHRLPRPASSSP 120

Query: 121 KDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDL 180
           KDIRAAAAKAA F          +DE++  A+S+LN +   SSA A KS GEIDD FFDL
Sbjct: 121 KDIRAAAAKAALF----------NDEQNPGAKSELNLNCCGSSAVAVKSGGEIDDTFFDL 180

Query: 181 PDLFL-HPNHQMDAFCFSQILPIDGFDSVFGRPPSP-SPSEYFAYRGSLL 229
           PDLF+  PNHQ+D FCFSQ +PIDGFDSVF RP SP SPS+YF YRGSLL
Sbjct: 181 PDLFIDDPNHQIDTFCFSQFVPIDGFDSVFTRPLSPSSPSDYFTYRGSLL 214

[Close]

BLAST of Lag0017463 vs. TAIR 10
Match: AT2G35700.1 (ERF family protein 38 )

HSP 1 Score: 163.3 bits (412), Expect = 2.3e-40
Identity = 93/149 (62.42%), Postives = 104/149 (69.80%), Query Frame = 0


Query: 46  KHPTYRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNF 105
           KHP +RGVRMRQWGKWVSEIREPKKKSRIWLGTFST EMAARAHDVAA  IKG SA+LNF
Sbjct: 41  KHPNFRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTAEMAARAHDVAALAIKGGSAHLNF 100

Query: 106 PELAHRLPIPASSSPKDIRAAAAKAASFSNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAA 165
           PELA+ LP PAS+ PKDI+AAAA AA+          D D E  +       + +SS A 
Sbjct: 101 PELAYHLPRPASADPKDIQAAAAAAAA------AVAIDMDVETSSPSPSPTVTETSSPAM 160

Query: 166 AAKSPGEIDDPFFDLPDLFLHPNHQMDAF 195
            A S    DD F DLPDL L+ NH +D F
Sbjct: 161 IALS----DDAFSDLPDLLLNVNHNIDGF 179

[Close]

BLAST of Lag0017463 vs. TAIR 10
Match: AT4G16750.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.1e-38
Identity = 94/175 (53.71%), Postives = 110/175 (62.86%), Query Frame = 0


Query: 18  SSSSSSSSDIKNSITEKNSKKLKLVND----GKHPTYRGVRMRQWGKWVSEIREPKKKSR 77
           SSS  S  ++++ + EK  K  K  N+     K P +RGVRMRQWGKWVSEIREP+KKSR
Sbjct: 4   SSSHESQRNLRSPVPEKTGKSSKTKNEQKGVSKQPNFRGVRMRQWGKWVSEIREPRKKSR 63

Query: 78  IWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSPKDIRAAAAKAASF 137
           IWLGTFSTPEMAARAHDVAA  IKG SA+LNFPELA+ LP PAS+ PKDI+ AAA AA+ 
Sbjct: 64  IWLGTFSTPEMAARAHDVAALAIKGGSAHLNFPELAYHLPRPASADPKDIQEAAAAAAAV 123

Query: 138 SNNLLLDPTDDDEEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDLPDLFLHPN 189
                                  ++P S S+    SP   DD F DLPDL L  N
Sbjct: 124 D---------------------WKAPESPSSTVTSSP-VADDAFSDLPDLLLDVN 156

[Close]

BLAST of Lag0017463 vs. TAIR 10
Match: AT2G44940.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 1.2e-36
Identity = 110/248 (44.35%), Postives = 132/248 (53.23%), Query Frame = 0


Query: 14  SSSSSSSSSSSSDIKNSITEKNSK-------------------------KLKLVNDG-KH 73
           SS ++SSSSSSS   N I E NSK                         K +  N G KH
Sbjct: 38  SSPTTSSSSSSSTNSNFIEEDNSKRKASRRSLSSLVSVEDDDDQNGGGGKRRKTNGGDKH 97

Query: 74  PTYRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPE 133
           PTYRGVRMR WGKWVSEIREP+KKSRIWLGT+ T EMAARAHDVAA  IKG +AYLNFP+
Sbjct: 98  PTYRGVRMRSWGKWVSEIREPRKKSRIWLGTYPTAEMAARAHDVAALAIKGTTAYLNFPK 157

Query: 134 LAHRLPIPASSSPKDIRAAAAKAA--------SFSNNLLLDPTD--------------DD 193
           LA  LP P ++SPKDI+AAA+ AA          SN+ + +  +              D 
Sbjct: 158 LAGELPRPVTNSPKDIQAAASLAAVNWQDSVNDVSNSEVAEIVEAEPSRAVVAQLFSSDT 217

Query: 194 EEDGAAQSDLNRSPSSSSAAAAKSPGEIDDPFFDLPDLFLHPNHQM---DAFC-FSQILP 210
                 QS      S +S +A       ++  FDLPDLF   N  M   DAFC +S    
Sbjct: 218 STTTTTQSQEYSEASCASTSACTDKDSEEEKLFDLPDLFTDENEMMIRNDAFCYYSSTWQ 277

[Close]

BLAST of Lag0017463 vs. TAIR 10
Match: AT3G60490.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 5.8e-36
Identity = 102/204 (50.00%), Postives = 125/204 (61.27%), Query Frame = 0


Query: 12  SQSSSSSSSSSSSSDIKNSITEKNS-----KKLKLVNDGKHPT-YRGVRMRQWGKWVSEI 71
           S SS  +SSS S S  K S+ + N      +K  + +D K+PT YRGVRMR WGKWVSEI
Sbjct: 27  SSSSVVTSSSDSWSTSKRSLVQDNDSGGKRRKSNVSDDNKNPTSYRGVRMRSWGKWVSEI 86

Query: 72  REPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPELAHRLPIPASSSPKDIRA 131
           REP+KKSRIWLGT+ T EMAARAHDVAA  IKG+S +LNFPEL+  LP P S SPKDI+A
Sbjct: 87  REPRKKSRIWLGTYPTAEMAARAHDVAALAIKGNSGFLNFPELSGLLPRPVSCSPKDIQA 146

Query: 132 AAAKAASFS-------NNLLLDPTDDDEEDGAAQSDLNRS---PSSSSAAAAKSPGEIDD 191
           AA KAA  +       +  L D     E    AQS  + S    S +S  ++      ++
Sbjct: 147 AATKAAEATTWHKPVIDKKLADELSHSELLSTAQSSTSSSFVFSSDTSETSSTDKESNEE 206

Query: 192 PFFDLPDLF----LHPNHQMDAFC 196
             FDLPDLF    ++PN   DAFC
Sbjct: 207 TVFDLPDLFTDGLMNPN---DAFC 227

[Close]

BLAST of Lag0017463 vs. TAIR 10
Match: AT1G77200.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 4.2e-34
Identity = 86/160 (53.75%), Postives = 102/160 (63.75%), Query Frame = 0


Query: 48  PTYRGVRMRQWGKWVSEIREPKKKSRIWLGTFSTPEMAARAHDVAARTIKGHSAYLNFPE 107
           P YRGVRMR WGKWVSEIREP+KKSRIWLGTFSTPEMAARAHD AA TIKG SA LNFPE
Sbjct: 41  PGYRGVRMRTWGKWVSEIREPRKKSRIWLGTFSTPEMAARAHDAAALTIKGTSAVLNFPE 100

Query: 108 LAHRLPIPASSSPKDIRAAAAKAA------SFSNNLLLDPTD-----DDEEDGAAQSDLN 167
           LA  LP PASSSP+D++AAAA AA      S S+ ++ DPT      + +   ++ S   
Sbjct: 101 LATYLPRPASSSPRDVQAAAAVAAAMDFSPSSSSLVVSDPTTVIAPAETQLSSSSYSTCT 160

Query: 168 RSPSSSSAAAAKSPGEIDDPFFDLPDLFLHPNHQMDAFCF 197
            S  S S+  A S  E      +LP L    +  +  F +
Sbjct: 161 SSSLSPSSEEAASTAEELSEIVELPSLETSYDESLSEFVY 200

The following BLAST results are available for this feature:

BLAST of Lag0017463 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905215.1	3.2e-97	85.59	ethylene-responsive transcription factor ERF039-like [Benincasa hispida]	[more]
XP_022965173.1	1.8e-87	76.89	ethylene-responsive transcription factor ERF039-like [Cucurbita maxima]	[more]
XP_022923459.1	5.2e-87	79.13	ethylene-responsive transcription factor ERF039-like [Cucurbita moschata]	[more]
XP_023552457.1	3.4e-86	76.05	ethylene-responsive transcription factor ERF039-like [Cucurbita pepo subsp. pepo...	[more]
XP_022945031.1	5.7e-86	79.28	ethylene-responsive transcription factor ERF038-like [Cucurbita moschata] >KAG65...	[more]

BLAST of Lag0017463 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQP3	3.2e-39	62.42	Ethylene-responsive transcription factor ERF038 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SUK8	3.0e-37	53.71	Ethylene-responsive transcription factor ERF039 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q8LBQ7	1.6e-35	44.35	Ethylene-responsive transcription factor ERF034 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9M210	8.2e-35	50.00	Ethylene-responsive transcription factor ERF035 OS=Arabidopsis thaliana OX=3702 ...	[more]
O80654	5.9e-33	53.75	Ethylene-responsive transcription factor ERF037 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Lag0017463 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HN35	8.6e-88	76.89	ethylene-responsive transcription factor ERF039-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1E6G7	2.5e-87	79.13	ethylene-responsive transcription factor ERF039-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1FZQ3	2.8e-86	79.28	ethylene-responsive transcription factor ERF038-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1L2H3	6.2e-86	79.73	ethylene-responsive transcription factor ERF038-like OS=Cucurbita maxima OX=3661...	[more]
A0A5A7TD44	5.2e-85	76.52	Ethylene-responsive transcription factor ERF039 OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of Lag0017463 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35700.1	2.3e-40	62.42	ERF family protein 38	[more]
AT4G16750.1	2.1e-38	53.71	Integrase-type DNA-binding superfamily protein	[more]
AT2G44940.1	1.2e-36	44.35	Integrase-type DNA-binding superfamily protein	[more]
AT3G60490.1	5.8e-36	50.00	Integrase-type DNA-binding superfamily protein	[more]
AT1G77200.1	4.2e-34	53.75	Integrase-type DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 50..61 score: 70.15 coord: 72..88 score: 66.2
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 49..112 e-value: 1.3E-36 score: 137.7
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 49..99 e-value: 5.0E-13 score: 49.1
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 49..106 score: 22.484093
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 50..108 e-value: 3.08643E-33 score: 112.75
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 49..107 e-value: 2.3E-24 score: 87.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..46
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 137..169
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..30
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 153..167
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..46
None	No IPR available	PANTHER	PTHR31985	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF042-RELATED	coord: 14..196
None	No IPR available	PANTHER	PTHR31985:SF130	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF043	coord: 14..196
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 49..107

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017463.1	Lag0017463.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity