Lag0017427 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017427
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	LINE-1 reverse transcriptase-like
Location	chr5: 3570973 .. 3571602 (+)
RNA-Seq Expression	Lag0017427
Synteny	Lag0017427

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGATCACCAAATTGATCGAGAAGATTATTAGAGACTTTATTTGGAGTGGAGGCTTCTACAAACCAGGATGCAGCCTTGTGAAATGGGAATGGACAGCCCTATCTACCAGCCACGGAGGTCTTGGGGTAAGTTCCTTGGTACACCGAAATAACTCCTTGTTAACCAAGTGGTTATGGCAATTTACTCAAAAAGGATCTTCTTTGTGGAGGAAAGTCATTGCTAGCTTATATATGGAGTTTCTTCATTTGGCTAGAAAACCAAGGAGTTGTGGAGGAAAGTCATTGCTAGCATATATATGGAGTTTCTTCATTTGGCTAGAAAACCAAGGAGTTAAAAAGAGGGAGTGCAACAGACTGTGGATAGATATTGCTACCAACAATTACCAAGTTCCAAACTATTTGCAGAAAGAACATAGATTTTGGGAGGATGTTTGGTGTGAAAGGGAACCTCTAAAAGATAGCTTCCCCGATTTGTACGTGATTTCGAATAAAAAAGGTGTTCCTGTTCACGATGGTTGGGATCATGATAATCAAGCTTGGAATCTAGGCATTCGAAGAAGATTATTTGACAGAGAGTTAAGTACTTTTTTTTTTAAAAGGAAACCATACTTTTCATTGAAAAAATGA

mRNA sequence

ATGGTGATCACCAAATTGATCGAGAAGATTATTAGAGACTTTATTTGGAGTGGAGGCTTCTACAAACCAGGATGCAGCCTTGTGAAATGGGAATGGACAGCCCTATCTACCAGCCACGGAGGTCTTGGGGTAAGTTCCTTGGTACACCGAAATAACTCCTTGTTAACCAAGTGGTTATGGCAATTTACTCAAAAAGGATCTTCTTTGTGGAGGAAAGTCATTGCTAGCTTATATATGGAGTTTCTTCATTTGGCTAGAAAACCAAGGAGTTGTGGAGGAAAGTCATTGCTAGCATATATATGGAGTTTCTTCATTTGGCTAGAAAACCAAGGAGTTAAAAAGAGGGAGTGCAACAGACTGTGGATAGATATTGCTACCAACAATTACCAAGTTCCAAACTATTTGCAGAAAGAACATAGATTTTGGGAGGATGTTTGGTGTGAAAGGGAACCTCTAAAAGATAGCTTCCCCGATTTGTACGTGATTTCGAATAAAAAAGGTGTTCCTGTTCACGATGGTTGGGATCATGATAATCAAGCTTGGAATCTAGGCATTCGAAGAAGATTATTTGACAGAGAGTTAAGTACTTTTTTTTTTAAAAGGAAACCATACTTTTCATTGAAAAAATGA

Coding sequence (CDS)

ATGGTGATCACCAAATTGATCGAGAAGATTATTAGAGACTTTATTTGGAGTGGAGGCTTCTACAAACCAGGATGCAGCCTTGTGAAATGGGAATGGACAGCCCTATCTACCAGCCACGGAGGTCTTGGGGTAAGTTCCTTGGTACACCGAAATAACTCCTTGTTAACCAAGTGGTTATGGCAATTTACTCAAAAAGGATCTTCTTTGTGGAGGAAAGTCATTGCTAGCTTATATATGGAGTTTCTTCATTTGGCTAGAAAACCAAGGAGTTGTGGAGGAAAGTCATTGCTAGCATATATATGGAGTTTCTTCATTTGGCTAGAAAACCAAGGAGTTAAAAAGAGGGAGTGCAACAGACTGTGGATAGATATTGCTACCAACAATTACCAAGTTCCAAACTATTTGCAGAAAGAACATAGATTTTGGGAGGATGTTTGGTGTGAAAGGGAACCTCTAAAAGATAGCTTCCCCGATTTGTACGTGATTTCGAATAAAAAAGGTGTTCCTGTTCACGATGGTTGGGATCATGATAATCAAGCTTGGAATCTAGGCATTCGAAGAAGATTATTTGACAGAGAGTTAAGTACTTTTTTTTTTAAAAGGAAACCATACTTTTCATTGAAAAAATGA

Protein sequence

MVITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQFTQKGSSLWRKVIASLYMEFLHLARKPRSCGGKSLLAYIWSFFIWLENQGVKKRECNRLWIDIATNNYQVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHDNQAWNLGIRRRLFDRELSTFFFKRKPYFSLKK

Homology

[Close]

BLAST of Lag0017427 vs. NCBI nr
Match: XP_022158566.1 (uncharacterized protein LOC111025019 [Momordica charantia])

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-26
Identity = 71/195 (36.41%), Postives = 100/195 (51.28%), Query Frame = 0


Query: 3   ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
           ITK + KI+RD  WSG       +LVKW+ ++L    GGLG+ +   +NN+LL KWLW F
Sbjct: 193 ITKEMNKIVRDLFWSGATSHGCKNLVKWDISSLPMKLGGLGIGAFPPKNNALLMKWLWHF 252

Query: 63  TQKGSSLWRKVIASLYMEFLHLARKPRSCGGKSLLAYIWSFFIWLENQGVKKRECNRLWI 122
           T++ ++LWR+VIAS+Y                 +  Y W        +  K +   R W 
Sbjct: 253 TREENALWRRVIASIY----------------GVDKYGWL------TKAPKGKAKGRPWF 312

Query: 123 DIATN--NYQVPNYLQKEH----RFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDH 182
           DIA N   +Q     +        FW D W    PL D FPD++ +S K+   V D W+ 
Sbjct: 313 DIAKNLSAFQEMVVFKANQGDRLSFWMDKWAGNLPLLDEFPDMFALSLKQNAYVKDCWNE 365

Query: 183 DNQAWNLGIRRRLFD 192
           +NQ W+L +RR LFD
Sbjct: 373 ENQTWDLALRRGLFD 365

[Close]

BLAST of Lag0017427 vs. NCBI nr
Match: KAG5579293.1 (hypothetical protein H5410_049920 [Solanum commersonii])

HSP 1 Score: 118.2 bits (295), Expect = 8.3e-23
Identity = 68/193 (35.23%), Postives = 97/193 (50.26%), Query Frame = 0


Query: 3   ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
           + + ++K+ R F+W G     G SLV WE   LS   GGLG+ +L  +N SLL KWLW++
Sbjct: 692 VVENLDKLRRKFLWQGNKDGKGYSLVNWETALLSKDRGGLGIKNLKLQNESLLKKWLWRY 751

Query: 63  TQKGSSLWRKVIASLYMEFLHLARKPRSCGGKSLLAYIWSFFIWLENQGVKK-RECNRLW 122
           T++ ++LW++VI + Y E       P            W      E  GV   R    LW
Sbjct: 752 TEERNTLWKEVIIAKYGEL-----NP------------WCSENTTEPYGVGAWRSIRSLW 811

Query: 123 IDIATNNYQVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHDNQAW 182
             + TN Y +      + +FW+DVW ++ PL+D FPDL+ I       V D W    Q W
Sbjct: 812 PQMETNLY-IKVGSGTKTKFWKDVWTDQSPLRDLFPDLFQICGNPDANVGDCW--TEQGW 864

Query: 183 NLGIRRRLFDREL 195
           +L  RR L D E+
Sbjct: 872 DLVFRRLLNDWEV 864

[Close]

BLAST of Lag0017427 vs. NCBI nr
Match: RVW94029.1 (LINE-1 reverse transcriptase-like [Vitis vinifera])

HSP 1 Score: 112.5 bits (280), Expect = 4.5e-21
Identity = 63/198 (31.82%), Postives = 91/198 (45.96%), Query Frame = 0


Query: 2   VITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQ 61
           ++ + +EK+ +DF+W G   +    LVKWE   +    GGLG+  LV  N +LL KW+W+
Sbjct: 778 LVARRLEKLQKDFLWGGANGENKAHLVKWEVVCVDKEKGGLGLRKLVCLNKALLGKWIWR 837

Query: 62  FTQKGSSLWRKVIASLY--MEFLHLARKPRSCGGKSLLAYIWSFFIWL-ENQGVKKRECN 121
           F +    LW+KV+ + Y   EF    RK     G  +   I     W  EN   K  + N
Sbjct: 838 FARAKEELWKKVLEAKYGQEEFGWRTRKANGVFGVGVWKEILKELAWCWENMVFKVGKGN 897

Query: 122 RLWIDIATNNYQVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHD- 181
           ++                   RFW D WC    L   FPDL+ ++ ++   V D WDH+ 
Sbjct: 898 KI-------------------RFWTDPWCGNNMLSQGFPDLFSMAAQRNATVEDYWDHNL 956

Query: 182 -NQAWNLGIRRRLFDREL 195
               WNL + R   D EL
Sbjct: 958 GQGGWNLRLLRDFNDWEL 956

[Close]

BLAST of Lag0017427 vs. NCBI nr
Match: RVW50579.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 110.5 bits (275), Expect = 1.7e-20
Identity = 63/197 (31.98%), Postives = 90/197 (45.69%), Query Frame = 0


Query: 3   ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
           + + ++K+ RDF+W GG  +    L+KWE      S GGLG+  LV  N +LL KW+W+F
Sbjct: 15  VARRLDKVQRDFLWGGGSEEKKTHLIKWEAICEDKSKGGLGLRKLVLLNKALLGKWVWRF 74

Query: 63  TQKGSSLWRKVIASLYMEFLHLARKPRSCG--GKSLLAYIWSFFIWL-ENQGVKKRECNR 122
                 LW++VI   Y +  H  R  ++ G  G  +   IW    W  +N G    + N+
Sbjct: 75  AIDRDDLWKQVIIEKYGQEGHEWRAKKAYGTIGVGVWKEIWKESDWCWDNMGFIVGKGNK 134

Query: 123 LWIDIATNNYQVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHD-- 182
           +                    FW DVWCE   L  SFP LY ++  +   V + WD +  
Sbjct: 135 I-------------------NFWTDVWCEDTRLSQSFPHLYAMAAHRDATVEEMWDQNFG 192

Query: 183 NQAWNLGIRRRLFDREL 195
              WNL   R   D EL
Sbjct: 195 QGGWNLRFLRDFNDWEL 192

[Close]

BLAST of Lag0017427 vs. NCBI nr
Match: RVW45036.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 110.5 bits (275), Expect = 1.7e-20
Identity = 62/197 (31.47%), Postives = 92/197 (46.70%), Query Frame = 0


Query: 3   ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
           + + ++K+ RDF+W GG  +    L+KWE      S GGLG+  LV  N +LL KW+W+F
Sbjct: 253 VARRLDKVQRDFLWGGGSEEKKAHLIKWEAICEDKSKGGLGLRKLVLLNKALLGKWVWRF 312

Query: 63  TQKGSSLWRKVIASLYMEFLHLARKPRSCG--GKSLLAYIWSFFIWL-ENQGVKKRECNR 122
                 LW++VI + Y +  H  R  ++ G  G  +   IW    W  +N G    + N+
Sbjct: 313 AIDRDDLWKQVIIAKYGQEGHGWRAKKAYGTIGVGVWKEIWKESDWCWDNMGFIVGKGNK 372

Query: 123 LWIDIATNNYQVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHD-- 182
           +                    FW DVWCE   L  SFP LY +++ +   V + W+ +  
Sbjct: 373 I-------------------NFWTDVWCEDARLSQSFPHLYAMASHRNATVEEMWNQNFG 430

Query: 183 NQAWNLGIRRRLFDREL 195
              WNL   R   D EL
Sbjct: 433 QGGWNLRFLRDFNDWEL 430

[Close]

BLAST of Lag0017427 vs. ExPASy TrEMBL
Match: A0A6J1E196 (uncharacterized protein LOC111025019 OS=Momordica charantia OX=3673 GN=LOC111025019 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 7.8e-27
Identity = 71/195 (36.41%), Postives = 100/195 (51.28%), Query Frame = 0


Query: 3   ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
           ITK + KI+RD  WSG       +LVKW+ ++L    GGLG+ +   +NN+LL KWLW F
Sbjct: 193 ITKEMNKIVRDLFWSGATSHGCKNLVKWDISSLPMKLGGLGIGAFPPKNNALLMKWLWHF 252

Query: 63  TQKGSSLWRKVIASLYMEFLHLARKPRSCGGKSLLAYIWSFFIWLENQGVKKRECNRLWI 122
           T++ ++LWR+VIAS+Y                 +  Y W        +  K +   R W 
Sbjct: 253 TREENALWRRVIASIY----------------GVDKYGWL------TKAPKGKAKGRPWF 312

Query: 123 DIATN--NYQVPNYLQKEH----RFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDH 182
           DIA N   +Q     +        FW D W    PL D FPD++ +S K+   V D W+ 
Sbjct: 313 DIAKNLSAFQEMVVFKANQGDRLSFWMDKWAGNLPLLDEFPDMFALSLKQNAYVKDCWNE 365

Query: 183 DNQAWNLGIRRRLFD 192
           +NQ W+L +RR LFD
Sbjct: 373 ENQTWDLALRRGLFD 365

[Close]

BLAST of Lag0017427 vs. ExPASy TrEMBL
Match: A0A0V0IIW1 (Putative ovule protein OS=Solanum chacoense OX=4108 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 7.5e-22
Identity = 67/193 (34.72%), Postives = 94/193 (48.70%), Query Frame = 0


Query: 3   ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
           I K I ++ R FIW G   K G +LVKWE   LS   GGLG+ +L  +NN LL KWLW+F
Sbjct: 36  IVKKINRMRRSFIWQGNKEKKGYNLVKWETLTLSRKQGGLGMRNLSIQNNCLLQKWLWRF 95

Query: 63  TQKGSSLWRKVIASLYMEFLHLARKPRSCGGKSLLAYIWSFFIWLENQGVKKRECNRLWI 122
             +  +LWR+ IA  Y  F     +         +   +   +W        +   RLW 
Sbjct: 96  CTEDGALWRRFIAGKYGLFSQWTTEE--------VMGSFGCCVW--------KTIRRLWP 155

Query: 123 DIATNNY-QVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHDNQAW 182
               N Y  V N L+ +  FW ++W   + L++ FP+LY +S ++   V   W    Q W
Sbjct: 156 QFNNNIYISVGNGLKTD--FWNEIWKGDDSLRNLFPNLYTLSLQRSATVAQVW--SQQGW 208

Query: 183 NLGIRRRLFDREL 195
           NL  RR L D E+
Sbjct: 216 NLVFRRALNDWEI 208

[Close]

BLAST of Lag0017427 vs. ExPASy TrEMBL
Match: A0A438IBH7 (LINE-1 reverse transcriptase-like OS=Vitis vinifera OX=29760 GN=LIN1_335 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.2e-21
Identity = 63/198 (31.82%), Postives = 91/198 (45.96%), Query Frame = 0


Query: 2   VITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQ 61
           ++ + +EK+ +DF+W G   +    LVKWE   +    GGLG+  LV  N +LL KW+W+
Sbjct: 778 LVARRLEKLQKDFLWGGANGENKAHLVKWEVVCVDKEKGGLGLRKLVCLNKALLGKWIWR 837

Query: 62  FTQKGSSLWRKVIASLY--MEFLHLARKPRSCGGKSLLAYIWSFFIWL-ENQGVKKRECN 121
           F +    LW+KV+ + Y   EF    RK     G  +   I     W  EN   K  + N
Sbjct: 838 FARAKEELWKKVLEAKYGQEEFGWRTRKANGVFGVGVWKEILKELAWCWENMVFKVGKGN 897

Query: 122 RLWIDIATNNYQVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHD- 181
           ++                   RFW D WC    L   FPDL+ ++ ++   V D WDH+ 
Sbjct: 898 KI-------------------RFWTDPWCGNNMLSQGFPDLFSMAAQRNATVEDYWDHNL 956

Query: 182 -NQAWNLGIRRRLFDREL 195
               WNL + R   D EL
Sbjct: 958 GQGGWNLRLLRDFNDWEL 956

[Close]

BLAST of Lag0017427 vs. ExPASy TrEMBL
Match: A0A803P465 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.2e-21
Identity = 70/210 (33.33%), Postives = 94/210 (44.76%), Query Frame = 0


Query: 3    ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
            +TK +EK++RDF+W G     G  LV W+        GGLG+  L  RN SLL KWLW+F
Sbjct: 1518 VTKALEKMMRDFLWEGSESSGGEHLVAWDEVCRPRHEGGLGIGRLELRNKSLLMKWLWRF 1577

Query: 63   TQKGSSLWRKVIASLYMEFLHLARKPRSCGGKSLLAYIWSFFIWLENQGVKKRECNRLWI 122
              + +SLW +V+ S Y    +L                     W   +G  +      W 
Sbjct: 1578 PLEQNSLWHRVVVSRYGRADNL---------------------WDSKKG-SRLSPKGPWR 1637

Query: 123  DIATNNYQVPNYLQKEH---------RFWEDVWCEREPLKDSFPDLYVISNKKGVPVHD- 182
            DI+        +L   H         RFWEDVW +  PL  +FPDL VIS  +  P+ + 
Sbjct: 1638 DISD---LYDEFLGLVHFKLGRGDRIRFWEDVWIDDYPLVSAFPDLAVISEGRNTPIKEL 1697

Query: 183  --------GWDHDNQAWNLGIRRRLFDREL 195
                    GW    ++WN   RR L DREL
Sbjct: 1698 IVDEGLPGGW---VESWNFKFRRNLMDREL 1699

[Close]

BLAST of Lag0017427 vs. ExPASy TrEMBL
Match: A0A438EB18 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_671 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 8.3e-21
Identity = 62/197 (31.47%), Postives = 92/197 (46.70%), Query Frame = 0


Query: 3   ITKLIEKIIRDFIWSGGFYKPGCSLVKWEWTALSTSHGGLGVSSLVHRNNSLLTKWLWQF 62
           + + ++K+ RDF+W GG  +    L+KWE      S GGLG+  LV  N +LL KW+W+F
Sbjct: 253 VARRLDKVQRDFLWGGGSEEKKAHLIKWEAICEDKSKGGLGLRKLVLLNKALLGKWVWRF 312

Query: 63  TQKGSSLWRKVIASLYMEFLHLARKPRSCG--GKSLLAYIWSFFIWL-ENQGVKKRECNR 122
                 LW++VI + Y +  H  R  ++ G  G  +   IW    W  +N G    + N+
Sbjct: 313 AIDRDDLWKQVIIAKYGQEGHGWRAKKAYGTIGVGVWKEIWKESDWCWDNMGFIVGKGNK 372

Query: 123 LWIDIATNNYQVPNYLQKEHRFWEDVWCEREPLKDSFPDLYVISNKKGVPVHDGWDHD-- 182
           +                    FW DVWCE   L  SFP LY +++ +   V + W+ +  
Sbjct: 373 I-------------------NFWTDVWCEDARLSQSFPHLYAMASHRNATVEEMWNQNFG 430

Query: 183 NQAWNLGIRRRLFDREL 195
              WNL   R   D EL
Sbjct: 433 QGGWNLRFLRDFNDWEL 430

The following BLAST results are available for this feature:

BLAST of Lag0017427 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158566.1	1.6e-26	36.41	uncharacterized protein LOC111025019 [Momordica charantia]	[more]
KAG5579293.1	8.3e-23	35.23	hypothetical protein H5410_049920 [Solanum commersonii]	[more]
RVW94029.1	4.5e-21	31.82	LINE-1 reverse transcriptase-like [Vitis vinifera]	[more]
RVW50579.1	1.7e-20	31.98	putative ribonuclease H protein [Vitis vinifera]	[more]
RVW45036.1	1.7e-20	31.47	putative ribonuclease H protein [Vitis vinifera]	[more]

BLAST of Lag0017427 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0017427 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E196	7.8e-27	36.41	uncharacterized protein LOC111025019 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A0V0IIW1	7.5e-22	34.72	Putative ovule protein OS=Solanum chacoense OX=4108 PE=4 SV=1	[more]
A0A438IBH7	2.2e-21	31.82	LINE-1 reverse transcriptase-like OS=Vitis vinifera OX=29760 GN=LIN1_335 PE=4 SV...	[more]
A0A803P465	2.2e-21	33.33	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A438EB18	8.3e-21	31.47	Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_671 P...	[more]

BLAST of Lag0017427 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017427.1	Lag0017427.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009987	cellular process
biological_process	GO:0050896	response to stimulus