Lag0017338 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017338
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Terpene cyclase/mutase family member
Location	chr5: 2454421 .. 2455046 (-)
RNA-Seq Expression	Lag0017338
Synteny	Lag0017338

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACCACTATTCATTGTTGCTTGTTGGGCTGACAATCCTAATGGTGAAGCTTACAAAAAGCATCTCGCTAGGATCAAAGATTACTTACGGCTTGGTGAAGATGGAATGAAGATGCAGGTTAGTAATATTGCTTAATGGATATATAATTTTTTGTTTGAGAATGTTGTATATATATAAACATCTATACACACACTCTAATTCTTCATATTTCTCACTGTATTAATATATATTCCTATAAGCAGAGTTTTGGTAGCCAGTCTTGGGATGTTGCTCTTGCCATTCAAGCAATTCTTGCAACCAATCTTCACCATGAATTCTCAGACACACTAAAGAAAGGTCATGACTTCATAAAAAAATCTCAGGTACGTAAGTTGTCCACACATGAACAATTATGAACATGTGCAACTAATATAGTTAACATTACACATTACTCTCTAGATGGTAATGTTTGTTGTTTTCTTGTTCTTTGGGTGCTCTTGTCCTCATAGCTTCTCAATGAATATTGGTGTCTTTTCGTGTTCTTTCTGTAGCTTTCTTTCGTATTTGTCTTTAGGGGTCTTGAGCCTCTCAATGAATCTTTCAGCTTCCTCTCTCTCTCAGTTAGGTGGTTTTTCAAAGTTTGA

mRNA sequence

ATGCAACCACTATTCATTGTTGCTTGTTGGGCTGACAATCCTAATGGTGAAGCTTACAAAAAGCATCTCGCTAGGATCAAAGATTACTTACGGCTTGGTGAAGATGGAATGAAGATGCAGAGTTTTGGTAGCCAGTCTTGGGATGTTGCTCTTGCCATTCAAGCAATTCTTGCAACCAATCTTCACCATGAATTCTCAGACACACTAAAGAAAGGTCATGACTTCATAAAAAAATCTCAGCTTTCTTTCGTATTTGTCTTTAGGGGTCTTGAGCCTCTCAATGAATCTTTCAGCTTCCTCTCTCTCTCAGTTAGGTGGTTTTTCAAAGTTTGA

Coding sequence (CDS)

ATGCAACCACTATTCATTGTTGCTTGTTGGGCTGACAATCCTAATGGTGAAGCTTACAAAAAGCATCTCGCTAGGATCAAAGATTACTTACGGCTTGGTGAAGATGGAATGAAGATGCAGAGTTTTGGTAGCCAGTCTTGGGATGTTGCTCTTGCCATTCAAGCAATTCTTGCAACCAATCTTCACCATGAATTCTCAGACACACTAAAGAAAGGTCATGACTTCATAAAAAAATCTCAGCTTTCTTTCGTATTTGTCTTTAGGGGTCTTGAGCCTCTCAATGAATCTTTCAGCTTCCTCTCTCTCTCAGTTAGGTGGTTTTTCAAAGTTTGA

Protein sequence

MQPLFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNLHHEFSDTLKKGHDFIKKSQLSFVFVFRGLEPLNESFSFLSLSVRWFFKV

Homology

[Close]

BLAST of Lag0017338 vs. NCBI nr
Match: XP_022150302.1 (beta-amyrin synthase-like isoform X2 [Momordica charantia])

HSP 1 Score: 159.8 bits (403), Expect = 1.3e-35
Identity = 71/80 (88.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 2   QPLFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNL 61
           +PLF+VACW+DNPNGEAYK+HLAR+KDYL +GEDGMKMQSFGSQSWDVAL+IQAILATNL
Sbjct: 369 KPLFMVACWSDNPNGEAYKRHLARVKDYLWVGEDGMKMQSFGSQSWDVALSIQAILATNL 428

Query: 62  HHEFSDTLKKGHDFIKKSQL 82
           HHEFSDTLKKGHDFIKKSQ+
Sbjct: 429 HHEFSDTLKKGHDFIKKSQI 448

[Close]

BLAST of Lag0017338 vs. NCBI nr
Match: XP_022150301.1 (beta-amyrin synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 159.8 bits (403), Expect = 1.3e-35
Identity = 71/80 (88.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 2   QPLFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNL 61
           +PLF+VACW+DNPNGEAYK+HLAR+KDYL +GEDGMKMQSFGSQSWDVAL+IQAILATNL
Sbjct: 369 KPLFMVACWSDNPNGEAYKRHLARVKDYLWVGEDGMKMQSFGSQSWDVALSIQAILATNL 428

Query: 62  HHEFSDTLKKGHDFIKKSQL 82
           HHEFSDTLKKGHDFIKKSQ+
Sbjct: 429 HHEFSDTLKKGHDFIKKSQI 448

[Close]

BLAST of Lag0017338 vs. NCBI nr
Match: XP_038890534.1 (LOW QUALITY PROTEIN: beta-amyrin synthase-like [Benincasa hispida])

HSP 1 Score: 154.5 bits (389), Expect = 5.5e-34
Identity = 70/81 (86.42%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MQPLFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATN 60
           ++PL +VACWADNPNGEAYKKHLAR+KDYL +GEDGMKMQSFGSQSWDVA AIQAILATN
Sbjct: 375 VEPLCMVACWADNPNGEAYKKHLARVKDYLWMGEDGMKMQSFGSQSWDVAFAIQAILATN 434

Query: 61  LHHEFSDTLKKGHDFIKKSQL 82
           LH EFSD LKKGHDFIKKSQ+
Sbjct: 435 LHDEFSDALKKGHDFIKKSQI 455

[Close]

BLAST of Lag0017338 vs. NCBI nr
Match: XP_022999281.1 (beta-amyrin synthase-like [Cucurbita maxima])

HSP 1 Score: 152.1 bits (383), Expect = 2.7e-33
Identity = 68/80 (85.00%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 2   QPLFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNL 61
           +PLF +ACW D+PNGEAYKKHLARIKDYL +GEDGMKMQSFGSQSWDVA AIQAILATNL
Sbjct: 376 KPLFTLACWVDDPNGEAYKKHLARIKDYLWVGEDGMKMQSFGSQSWDVAFAIQAILATNL 435

Query: 62  HHEFSDTLKKGHDFIKKSQL 82
           HHEFS+TLK+GHDFIK+SQ+
Sbjct: 436 HHEFSETLKRGHDFIKQSQV 455

[Close]

BLAST of Lag0017338 vs. NCBI nr
Match: XP_038890187.1 (beta-amyrin synthase-like isoform X7 [Benincasa hispida])

HSP 1 Score: 151.8 bits (382), Expect = 3.6e-33
Identity = 68/80 (85.00%), Postives = 75/80 (93.75%), Query Frame = 0


Query: 2   QPLFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNL 61
           +PL ++ACWAD+PNGEAYKKHLAR+KDYL +GEDGMKMQSFGSQSWD A AIQAILATNL
Sbjct: 372 KPLCMIACWADDPNGEAYKKHLARVKDYLWVGEDGMKMQSFGSQSWDAAFAIQAILATNL 431

Query: 62  HHEFSDTLKKGHDFIKKSQL 82
           H EFSDTLKKGHDFIKKSQ+
Sbjct: 432 HEEFSDTLKKGHDFIKKSQI 451

[Close]

BLAST of Lag0017338 vs. ExPASy Swiss-Prot
Match: E7DN63 (Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 7.5e-26
Identity = 50/79 (63.29%), Postives = 66/79 (83.54%), Query Frame = 0


Query: 4   LFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNLHH 63
           L ++ACW ++PNG+ +KKHLARI DYL + EDGMKMQSFGSQ WD   AIQA+LA+ ++ 
Sbjct: 374 LCMLACWVEDPNGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFAIQALLASEMND 433

Query: 64  EFSDTLKKGHDFIKKSQLS 83
           E +DTL+KGHDFIK+SQ++
Sbjct: 434 EIADTLRKGHDFIKQSQVT 452

[Close]

BLAST of Lag0017338 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL6 (Beta-amyrin synthase 1 OS=Kalopanax septemlobus OX=228393 GN=BAS1 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 7.5e-26
Identity = 51/78 (65.38%), Postives = 63/78 (80.77%), Query Frame = 0


Query: 4   LFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNLHH 63
           L ++ACW ++PNG+ +KKHLARI DY+ +GEDGMKMQSFGSQ WD    IQA+LA++L H
Sbjct: 376 LCMLACWVEDPNGDYFKKHLARIPDYIWVGEDGMKMQSFGSQEWDTGFGIQALLASDLTH 435

Query: 64  EFSDTLKKGHDFIKKSQL 82
           E   TL KGHDFIKKSQ+
Sbjct: 436 ELGPTLMKGHDFIKKSQV 453

[Close]

BLAST of Lag0017338 vs. ExPASy Swiss-Prot
Match: A8CDT2 (Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 7.5e-26
Identity = 51/78 (65.38%), Postives = 63/78 (80.77%), Query Frame = 0


Query: 4   LFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNLHH 63
           L ++ACW ++PNG+ +KKHLARI DY+ + EDGMKMQSFGSQ WD   AIQA+LATNL  
Sbjct: 375 LCMLACWVEDPNGDYFKKHLARIPDYIWVAEDGMKMQSFGSQEWDTGFAIQALLATNLTD 434

Query: 64  EFSDTLKKGHDFIKKSQL 82
           E  D L++GHDFIKKSQ+
Sbjct: 435 EIGDVLRRGHDFIKKSQV 452

[Close]

BLAST of Lag0017338 vs. ExPASy Swiss-Prot
Match: Q9MB42 (Beta-amyrin synthase OS=Glycyrrhiza glabra OX=49827 GN=GgbAS1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 9.7e-26
Identity = 51/78 (65.38%), Postives = 63/78 (80.77%), Query Frame = 0


Query: 4   LFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNLHH 63
           L ++ACW ++PNG+A+KKHLAR+ DYL + EDGM MQSFGSQ WD   A+QA+LATNL  
Sbjct: 375 LCMLACWVEDPNGDAFKKHLARVPDYLWVSEDGMTMQSFGSQEWDAGFAVQALLATNLVE 434

Query: 64  EFSDTLKKGHDFIKKSQL 82
           E + TL KGHDFIKKSQ+
Sbjct: 435 EIAPTLAKGHDFIKKSQV 452

[Close]

BLAST of Lag0017338 vs. ExPASy Swiss-Prot
Match: Q2XPU7 (Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.7e-25
Identity = 49/79 (62.03%), Postives = 67/79 (84.81%), Query Frame = 0


Query: 2   QPLFIVACWADNPNGEAYKKHLARIKDYLRLGEDGMKMQSFGSQSWDVALAIQAILATNL 61
           +PL ++ACW ++P+GEA+KKHLARI DY+ +GEDG+KMQSFGSQ+WD +LA+QA++A++L
Sbjct: 372 KPLCMLACWIEDPHGEAFKKHLARIADYIWVGEDGIKMQSFGSQTWDTSLALQALIASDL 431

Query: 62  HHEFSDTLKKGHDFIKKSQ 81
            HE   TLK+GH F K SQ
Sbjct: 432 SHEIGPTLKQGHVFTKNSQ 450

The following BLAST results are available for this feature:

BLAST of Lag0017338 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150302.1	1.3e-35	88.75	beta-amyrin synthase-like isoform X2 [Momordica charantia]	[more]
XP_022150301.1	1.3e-35	88.75	beta-amyrin synthase-like isoform X1 [Momordica charantia]	[more]
XP_038890534.1	5.5e-34	86.42	LOW QUALITY PROTEIN: beta-amyrin synthase-like [Benincasa hispida]	[more]
XP_022999281.1	2.7e-33	85.00	beta-amyrin synthase-like [Cucurbita maxima]	[more]
XP_038890187.1	3.6e-33	85.00	beta-amyrin synthase-like isoform X7 [Benincasa hispida]	[more]

BLAST of Lag0017338 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
E7DN63	7.5e-26	63.29	Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1	[more]
A0A0S2IHL6	7.5e-26	65.38	Beta-amyrin synthase 1 OS=Kalopanax septemlobus OX=228393 GN=BAS1 PE=1 SV=1	[more]
A8CDT2	7.5e-26	65.38	Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1	[more]
Q9MB42	9.7e-26	65.38	Beta-amyrin synthase OS=Glycyrrhiza glabra OX=49827 GN=GgbAS1 PE=1 SV=1	[more]
Q2XPU7	1.7e-25	62.03	Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.50.10.20		coord: 18..99 e-value: 4.1E-16 score: 60.7
None	No IPR available	PANTHER	PTHR11764:SF58	LUPEOL SYNTHASE 1	coord: 3..81
IPR018333	Squalene cyclase	PANTHER	PTHR11764	TERPENE CYCLASE/MUTASE FAMILY MEMBER	coord: 3..81
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 17..83

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017338.1	Lag0017338.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016104	triterpenoid biosynthetic process
cellular_component	GO:0005811	lipid droplet
molecular_function	GO:0042300	beta-amyrin synthase activity
molecular_function	GO:0000250	lanosterol synthase activity
molecular_function	GO:0016866	intramolecular transferase activity