Lag0017309 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0017309 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 109.8 bits (273), Expect = 3.3e-20
Identity = 70/203 (34.48%), Postives = 100/203 (49.26%), Query Frame = 0

Query: 1   MWLCSKFSQLYQSLY---HLDLVDVIWALREKLGALDFELVTVFWWSVWNLRNSLCWRGE 60
           +W CSKFS     L+     D+   +    + +       + V  W++WN RN       
Sbjct: 4   IWQCSKFSHFLHLLWVPVGSDMGHFMRVWTDLVSWQHIGAIVVLLWAIWNARNQTPQHFS 63

Query: 61  SDG--RDLWAWSEEYLRAYHDVVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLNTDAS 120
             G   DL +WSE YL+ Y     QR S  +L  C  RP   + W PP     K+N DA+
Sbjct: 64  LGGSLSDLVSWSENYLQVYQ--AAQRSSXSALVVC--RPPRVAIWRPPAAPLLKVNVDAA 123

Query: 121 VRPDKGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDLAEGWALVKGVELALQMCFLSFCV 180
            R +   AG G ++RD +G V L A   L R   VD  EG+A+ +G+ LA++  F+ F +
Sbjct: 124 FRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQI 183

Query: 181 EVDSLRLVRILHGEVTDSSEVAV 199
           E DSLR+  +L  +  D SEV V
Sbjct: 184 ETDSLRIFNLLTTDCVDDSEVGV 202

BLAST of Lag0017309 vs. NCBI nr
Match: TXG68182.1 (hypothetical protein EZV62_003117 [Acer yangbiense])

HSP 1 Score: 88.2 bits (217), Expect = 1.0e-13
Identity = 48/176 (27.27%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 34  DFELVTVFWWSVWNLRNSLCWRGESDGRD-LWAWSEEYLRAYHDVVGQRESRCSLQPCPS 93
           +  L+ +FWW +W LRN+ C   +  G D L AW E +L A+ D      ++  L    +
Sbjct: 304 ELALLCIFWWRIWFLRNNNCHGKKGIGDDSLLAWCESFLVAFWDA-----NQVCLNGVSA 363

Query: 94  RPVEQSSWTPPMGGGFKLNTDASVRPDKGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDL 153
                  W PP+ G  K+N DA++  ++G  G G V+RD  G VL +    +   +S  +
Sbjct: 364 ANPSIVVWKPPVEGTIKINCDAALDMNRGVVGFGLVVRDFGGVVLASCVQHIQVNFSPLI 423

Query: 154 AEGWALVKGVELALQMCFLSFCVEVDSLRLVRILHGEVTDSSEVAVEFGQFENGSD 209
           AE   +++G+ LA     + F ++ D+L +V+++  +    +++ +  G+  +  D
Sbjct: 424 AEAMTILRGINLAFDSNLIPFVIKTDALAVVKLIEAKSPSLADIGLVIGEIVSKLD 474

BLAST of Lag0017309 vs. NCBI nr
Match: XP_015385025.1 (uncharacterized protein LOC107176703 [Citrus sinensis])

HSP 1 Score: 87.8 bits (216), Expect = 1.3e-13
Identity = 52/197 (26.40%), Postives = 98/197 (49.75%), Query Frame = 0

Query: 1   MWLCSKFSQLYQSLYHLDLVDVIWALREKLGALDFELVTVFWWSVWNLRNSLCWRGES-D 60
           +W  S  +   Q +   D++ V+ +L +K+   + E V V WW VW+ RN L + G+  +
Sbjct: 9   VWKYSYLASEMQGVVREDILSVMQSLIKKMSMREVEFVAVVWWEVWHARNKLIFEGKRVN 68

Query: 61  GRDLWAWSEEYLRAYHDVVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLNTDASVRPD 120
              L A ++  + AY  V G+ ++    +       +Q  W PP  G +K+N DA+V  +
Sbjct: 69  PMSLIAKAQAAVEAYQRVHGKEQTSKRSEDVS----KQDQWCPPPVGFYKVNVDAAVHTE 128

Query: 121 KGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDLAEGWALVKGVELALQMCFLSFCVEVDS 180
           +   G G V+R+  G +++AA        +V  AE  A+  G+E+AL+    +  +E D 
Sbjct: 129 QQLTGLGVVIRNPQGQIIVAAIKSTKFQDNVTAAEAEAVKWGLEIALEARLAAVIIETDC 188

Query: 181 LRLVRILHGEVTDSSEV 197
           + +  + + + +   E+
Sbjct: 189 IEVANLANSKTSSKKEI 201

BLAST of Lag0017309 vs. NCBI nr
Match: XP_024033533.1 (uncharacterized protein LOC112095662 [Citrus clementina])

HSP 1 Score: 87.4 bits (215), Expect = 1.7e-13
Identity = 51/197 (25.89%), Postives = 94/197 (47.72%), Query Frame = 0

Query: 1   MWLCSKFSQLYQSLYHLDLVDVIWALREKLGALDFELVTVFWWSVWNLRNSLCWRGES-D 60
           +W C+      QS+   D++  + +L  K    + + V   WW+ W+ RN L + G+  D
Sbjct: 9   IWRCTDLEAAVQSIRRDDMLSTMHSLMRKGAKSEIDYVASIWWATWHARNKLLFEGKKPD 68

Query: 61  GRDLWAWSEEYLRAYHDVVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLNTDASVRPD 120
            R+  A ++  + AY  +  + +      P   +  E   W PP     K+N DA+V  +
Sbjct: 69  PRETVAKAKAIVVAYQAMQFKEQD----SPSSIKEKEAQRWNPPPSSKLKINVDAAVHAE 128

Query: 121 KGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDLAEGWALVKGVELALQMCFLSFCVEVDS 180
              AG G V+R+  G V++AA   +     V + E  A+  G+E+A +  F +  VE D 
Sbjct: 129 SQMAGLGAVIRNSQGQVVVAAVKSINFQGDVSIVEAKAVQWGLEVASKASFTNVIVETDC 188

Query: 181 LRLVRILHGEVTDSSEV 197
             +  + + +V++ +E+
Sbjct: 189 SMVANLANNKVSNKTEI 201

BLAST of Lag0017309 vs. NCBI nr
Match: RYE02783.1 (reverse transcriptase-like protein, partial [Rickettsiaceae bacterium])

HSP 1 Score: 87.0 bits (214), Expect = 2.3e-13
Identity = 56/180 (31.11%), Postives = 86/180 (47.78%), Query Frame = 0

Query: 18  DLVDVIWALREKLGALDFELVTVFWWSVWNLRNS-LCWRGESDGRDLWAWSEEYLRAYHD 77
           D  D I    E L     EL     W +WN RN+ L  R       L  W+  +L  Y  
Sbjct: 53  DAFDAILRATETLEVRFHELFITIIWELWNHRNNVLAGRSAWSQPQLVQWTVSFLEEY-- 112

Query: 78  VVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLNTDASVRPDKGEAGGGCVLRDVSGAV 137
               R +     P  +R      WTPP  G   +N DA++  D+G  G GC++RD +G V
Sbjct: 113 ----RAATVGETPTSTRVTTTPRWTPPRDGTLSINVDAALARDRGGFGVGCIVRDHTGEV 172

Query: 138 LLAACLDLPRCWSVDLAEGWALVKGVELALQMCFLSFCVEVDSLRLVRILHGEVTDSSEV 197
           + +  L L R +S  LAE +A+++G+EL   +      ++ D L +VR++   VT ++E+
Sbjct: 173 IASQTLFLARGFSPQLAEAYAVLQGIELVRSVGGRRAEIQSDCLGVVRMIDEGVTPATEL 226

BLAST of Lag0017309 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.8e-06
Identity = 52/178 (29.21%), Postives = 76/178 (42.70%), Query Frame = 0

Query: 26  LREKLGALDFELVTVF----WWSVWNLRNSLCWRGESDGRD----LWAWSEEYLRAY--H 85
           L ++ G  D    T+F    WW  W  R    +   +  RD    +  W+ E  RA+  +
Sbjct: 378 LGDRSGCEDIPWSTIFAVIIWWG-WKWRCGNIFGENTKCRDRVKFVKEWAVEVYRAHSGN 437

Query: 86  DVVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLNTDASVRPDKGEAGGGCVLRDVSGA 145
            +VG  + R               W  P  G  K+NTD + R + G A  G VLRD +GA
Sbjct: 438 VLVGITQPRVE---------RMIGWVSPCVGWVKVNTDGASRGNPGLASAGGVLRDCTGA 497

Query: 146 VLLAACLDLPRCWSVDLAEGWALVKGVELALQMCFLSFCVEVDSLRLVRILHGEVTDS 194
                 L++ RC S   AE W +  G+  A +       +EVDS  +V  L   ++DS
Sbjct: 498 WCGGFSLNIGRC-SAPQAELWGVYYGLYFAWEKKVPRVELEVDSEVIVGFLKTGISDS 544

BLAST of Lag0017309 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.6e-20
Identity = 70/203 (34.48%), Postives = 100/203 (49.26%), Query Frame = 0

Query: 1   MWLCSKFSQLYQSLY---HLDLVDVIWALREKLGALDFELVTVFWWSVWNLRNSLCWRGE 60
           +W CSKFS     L+     D+   +    + +       + V  W++WN RN       
Sbjct: 4   IWQCSKFSHFLHLLWVPVGSDMGHFMRVWTDLVSWQHIGAIVVLLWAIWNARNQTPQHFS 63

Query: 61  SDG--RDLWAWSEEYLRAYHDVVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLNTDAS 120
             G   DL +WSE YL+ Y     QR S  +L  C  RP   + W PP     K+N DA+
Sbjct: 64  LGGSLSDLVSWSENYLQVYQ--AAQRSSXSALVVC--RPPRVAIWRPPAAPLLKVNVDAA 123

Query: 121 VRPDKGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDLAEGWALVKGVELALQMCFLSFCV 180
            R +   AG G ++RD +G V L A   L R   VD  EG+A+ +G+ LA++  F+ F +
Sbjct: 124 FRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQI 183

Query: 181 EVDSLRLVRILHGEVTDSSEVAV 199
           E DSLR+  +L  +  D SEV V
Sbjct: 184 ETDSLRIFNLLTTDCVDDSEVGV 202

BLAST of Lag0017309 vs. ExPASy TrEMBL
Match: A0A5C7IHD9 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_003117 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.9e-14
Identity = 48/176 (27.27%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 34  DFELVTVFWWSVWNLRNSLCWRGESDGRD-LWAWSEEYLRAYHDVVGQRESRCSLQPCPS 93
           +  L+ +FWW +W LRN+ C   +  G D L AW E +L A+ D      ++  L    +
Sbjct: 304 ELALLCIFWWRIWFLRNNNCHGKKGIGDDSLLAWCESFLVAFWDA-----NQVCLNGVSA 363

Query: 94  RPVEQSSWTPPMGGGFKLNTDASVRPDKGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDL 153
                  W PP+ G  K+N DA++  ++G  G G V+RD  G VL +    +   +S  +
Sbjct: 364 ANPSIVVWKPPVEGTIKINCDAALDMNRGVVGFGLVVRDFGGVVLASCVQHIQVNFSPLI 423

Query: 154 AEGWALVKGVELALQMCFLSFCVEVDSLRLVRILHGEVTDSSEVAVEFGQFENGSD 209
           AE   +++G+ LA     + F ++ D+L +V+++  +    +++ +  G+  +  D
Sbjct: 424 AEAMTILRGINLAFDSNLIPFVIKTDALAVVKLIEAKSPSLADIGLVIGEIVSKLD 474

BLAST of Lag0017309 vs. ExPASy TrEMBL
Match: A0A4Q3DBE2 (Reverse transcriptase-like protein (Fragment) OS=Rickettsiaceae bacterium OX=2023360 GN=EOP33_09975 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.1e-13
Identity = 56/180 (31.11%), Postives = 86/180 (47.78%), Query Frame = 0

Query: 18  DLVDVIWALREKLGALDFELVTVFWWSVWNLRNS-LCWRGESDGRDLWAWSEEYLRAYHD 77
           D  D I    E L     EL     W +WN RN+ L  R       L  W+  +L  Y  
Sbjct: 53  DAFDAILRATETLEVRFHELFITIIWELWNHRNNVLAGRSAWSQPQLVQWTVSFLEEY-- 112

Query: 78  VVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLNTDASVRPDKGEAGGGCVLRDVSGAV 137
               R +     P  +R      WTPP  G   +N DA++  D+G  G GC++RD +G V
Sbjct: 113 ----RAATVGETPTSTRVTTTPRWTPPRDGTLSINVDAALARDRGGFGVGCIVRDHTGEV 172

Query: 138 LLAACLDLPRCWSVDLAEGWALVKGVELALQMCFLSFCVEVDSLRLVRILHGEVTDSSEV 197
           + +  L L R +S  LAE +A+++G+EL   +      ++ D L +VR++   VT ++E+
Sbjct: 173 IASQTLFLARGFSPQLAEAYAVLQGIELVRSVGGRRAEIQSDCLGVVRMIDEGVTPATEL 226

BLAST of Lag0017309 vs. ExPASy TrEMBL
Match: A0A7J6GAB1 (RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_021018 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 9.3e-13
Identity = 58/198 (29.29%), Postives = 92/198 (46.46%), Query Frame = 0

Query: 1   MWLCSKFSQLYQSLYHLDLV--------DVIWALREKLGALDFELVTVFWWSVWNLRNSL 60
           +W CSK   L++    L L         D ++ ++++     F L     W +W+ RN  
Sbjct: 220 LWYCSKTQALWKHFGFLHLFPPNISMAPDFLFIMKDRCSKDTFILFLGVTWLIWHRRNKS 279

Query: 61  CWRGESDGRDLW-AWSEEYLRAYHDVVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLN 120
            ++  +    +W  W+   L    D   Q      L      PV   SW+PP  G FK+N
Sbjct: 280 IFQQRNFDNKVWIEWASNLL----DYQLQLVPTPPLHSLKKAPV---SWSPPPSGTFKIN 339

Query: 121 TDASVRPDKGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDLAEGWALVKGVELALQMCFL 180
           TDAS+   +   G G V+RD  G V++A  + +P C SV++AE  A+  G+ LA +    
Sbjct: 340 TDASLIAGQPGCGLGVVIRDHLGIVVVAETVYIPGCLSVNMAESLAIHSGLLLANRWSLS 399

Query: 181 SFCVEVDSLRLVRILHGE 190
           S C+  D   ++  L+GE
Sbjct: 400 SICISSDCQPVIHALNGE 410

BLAST of Lag0017309 vs. ExPASy TrEMBL
Match: A0A803Q8X4 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.6e-12
Identity = 53/198 (26.77%), Postives = 93/198 (46.97%), Query Frame = 0

Query: 1    MWLCSKFSQLYQSLYHLDLV--------DVIWALREKLGALDFELVTVFWWSVWNLRNSL 60
            +W C K   L++    L L         D ++ ++++     F L     W +W+ RN  
Sbjct: 854  LWYCPKTQDLWKHFGFLHLFLPNIGKAPDFLFIMKDRCSKETFILFLGVTWLIWHRRNKC 913

Query: 61   CWRGESDGRDLW-AWSEEYLRAYHDVVGQRESRCSLQPCPSRPVEQSSWTPPMGGGFKLN 120
             ++ ++    +W  W+   +    D   Q +S   L P    PV   SW+PP  G F +N
Sbjct: 914  IFQQKNFDNKVWIEWASNLI----DFQLQTDSTPPLPPLKKSPV---SWSPPPSGTFMIN 973

Query: 121  TDASVRPDKGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDLAEGWALVKGVELALQMCFL 180
             D S+   +   G G V+RD  GAV++A  + +P C S ++AE  A+  G++LA +    
Sbjct: 974  ADVSLIVGQPGCGLGVVIRDHLGAVVVAETIFIPSCLSFNMAESLAIRSGLKLADRWSLS 1033

Query: 181  SFCVEVDSLRLVRILHGE 190
            + C+  D   ++  L+G+
Sbjct: 1034 TICISSDCQSVIHALNGD 1044

BLAST of Lag0017309 vs. TAIR 10
Match: AT5G42905.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-07
Identity = 51/160 (31.87%), Postives = 71/160 (44.38%), Query Frame = 0

Query: 40  VFWWSVWNLRNSLCWRGESDGRD----LWAWSEEYLRAY--HDVVGQRESRCSLQPCPSR 99
           + WW  W  R S  +   +  RD    +  W  E  RA+  + +VG      S QP   R
Sbjct: 84  IIWWG-WKWRCSNIFGENTKCRDRVKFVKEWVVEVYRAHLGNALVG------STQPRVER 143

Query: 100 PVEQSSWTPPMGGGFKLNTDASVRPDKGEAGGGCVLRDVSGAVLLAACLDLPRCWSVDLA 159
            +    W  P  G  K+NTD + R + G A  G VLRD  GA      L++ RC S   A
Sbjct: 144 LI---GWVLPCVGWVKVNTDGASRGNPGLASAGGVLRDCEGAWCGGFSLNIGRC-SAQHA 203

Query: 160 EGWALVKGVELALQMCFLSFCVEVDSLRLVRILHGEVTDS 194
           E W +  G+  A +       +EVDS  +V  L   ++DS
Sbjct: 204 ELWGVYYGLYFAWEKKVPRVELEVDSEAIVGFLKTGISDS 232