Lag0017289 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017289
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	UDP-N-acetylglucosamine transferase subunit ALG14
Location	chr5: 1784046 .. 1785733 (+)
RNA-Seq Expression	Lag0017289
Synteny	Lag0017289

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTCAAGAATTGGAGAGTTCAATCTACCTAGGAATTTGGTGCTGTGCTATGAGTCAGGCACTCAAACTACAGGTTTGTCTTTCTGAAGCAGAAGCATCATTTGGTAGTTGTGTTTAATGGACTATTCCTTGGCCAGAAGAATATGTGGCCATGAAATAGGGATTAAGTAGAATAGGAAGATTGGATTTATTGGCGAGTGAAAATGTTTCCTAGACTAAAATTCGTTGAAGTGCAAACTTGAGTCAAAGGATCATTCCAGCTTGTTTAAGAAGATTTATGATAATGCCCTACAAAATGGAGCTTCAGGTGATTTTGGAGTTTCTTCAACTTTCACTGACTGACACATGAGGATGTTCTACTAGACCCAGTGGCAAATTCTTTTCAACTAGGGGCAAATGTTGCGTGTCAGAAAGCACACGTTCAATACAAAGTAACCAGTAATAGCACAGTGGAGATCAAAGTAAATTTTCTTGCAGATTTGGTCCACAAACTTGAATGGAATCAATCAGGATTTTGGTGCTTGAGTTCGAATTGGACATAGGCTGCCACATGCATCGTACGTACACTTGGTTTGGGAAAACAGTAACAACCATGAAACTCTTTTAGAAGATCAGTAATGGATTCTCTTGGAATGTTGTCAATTAGTAGTGAGGTTCTCATCCAAGCTTGAACTTGTTAGTTTTCTTCAGATAGTAAGTTACGGTCTCCCCATATTCTCAGCTTCCAGGAGACAGCCCCTGGACCTTAGATTGATGAATCTAGTTTATTTTCCTTTTTTCCTTTTTTTCGCTCCAGGCACGTATTGCTGTCTTCAGAAATCTCTGTTGCCCTTTTATTTTAATACAGTGATTTTCCCGCATAATTTTTCTATCATACAATCTTGATCATTGTCAAATAAAAACTCTAAGAGTCAACTGCAAATTATGGATGCCACTATTAACAATATTTTCTGTAGCCACAAGTTAAACTAGATTCCCTTCTAACAATATATTGGACATTTTGTTTCATTTTTCTCTCTCTCGCATAATTTATGAGTTTTCCAATTAAATTTATCTGTGAATTGGCGCTTGTTGCAGACTGGGGCTGAGGTAGACAAGATTGCACAGTTTATGCAGATCTACAGGAGTAGGGAAGTAGGACAATCATATTTCACTTCTGTATGGACAACATTATTTGCCACATTGCACGCACTCTGGCTAATGATTAAAATTAGACCTCAAGTGGTATTTCTTCTGTCTGTAAATCTGCTTATTTGTATGACCTCTTTTTCTTAAGGATCCGTTGTTGTGATCGTCGTCGTTCTAGCCCATGTTTTTTTTTTTTCTCTTCTTTTTCTTAACCAGTTGTCTTGTTGTAGATTCTCTGCAATGGCCCTGGTACCTGTATCCCTCTATGCGTAATTGCATTCATATTCAAGGTTCAAAAGTGCACTAAACAGCATTTTTTTGCATTTTCCAATTCCAAGATCATAATATTCTAGATGATAAACACTGGAATCTGAATGCAGGTTGTGGGGATAAGATGGTCGTCAATTTTTTATGTGGAAAGCATTGCAAGAGTGCAGAGATTATCACTGAGTGGCTTGATTTTGTACAAGTTATACATGGCTGATCAGTTCTTTGTGCAATGGCCACAACTACAGAGAAAATATCCTCGAGCTCACTACGTCGGTTGTCTCATGTAG

mRNA sequence

ATGTGTTCAAGAATTGGAGAGTTCAATCTACCTAGGAATTTGGTGCTGTGCTATGAGTCAGGCACTCAAACTACAGACCCAGTGGCAAATTCTTTTCAACTAGGGGCAAATGTTGCGTGTCAGAAAGCACACGTTCAATACAAAGTAACCAGTAATAGCACAGTGGAGATCAAAACTGGGGCTGAGGTAGACAAGATTGCACAGTTTATGCAGATCTACAGGAGTAGGGAAGTAGGACAATCATATTTCACTTCTGTATGGACAACATTATTTGCCACATTGCACGCACTCTGGCTAATGATTAAAATTAGACCTCAAGTGATTCTCTGCAATGGCCCTGGTACCTGTATCCCTCTATGCGTAATTGCATTCATATTCAAGGTTGTGGGGATAAGATGGTCGTCAATTTTTTATGTGGAAAGCATTGCAAGAGTGCAGAGATTATCACTGAGTGGCTTGATTTTGTACAAGTTATACATGGCTGATCAGTTCTTTGTGCAATGGCCACAACTACAGAGAAAATATCCTCGAGCTCACTACGTCGGTTGTCTCATGTAG

Coding sequence (CDS)

ATGTGTTCAAGAATTGGAGAGTTCAATCTACCTAGGAATTTGGTGCTGTGCTATGAGTCAGGCACTCAAACTACAGACCCAGTGGCAAATTCTTTTCAACTAGGGGCAAATGTTGCGTGTCAGAAAGCACACGTTCAATACAAAGTAACCAGTAATAGCACAGTGGAGATCAAAACTGGGGCTGAGGTAGACAAGATTGCACAGTTTATGCAGATCTACAGGAGTAGGGAAGTAGGACAATCATATTTCACTTCTGTATGGACAACATTATTTGCCACATTGCACGCACTCTGGCTAATGATTAAAATTAGACCTCAAGTGATTCTCTGCAATGGCCCTGGTACCTGTATCCCTCTATGCGTAATTGCATTCATATTCAAGGTTGTGGGGATAAGATGGTCGTCAATTTTTTATGTGGAAAGCATTGCAAGAGTGCAGAGATTATCACTGAGTGGCTTGATTTTGTACAAGTTATACATGGCTGATCAGTTCTTTGTGCAATGGCCACAACTACAGAGAAAATATCCTCGAGCTCACTACGTCGGTTGTCTCATGTAG

Protein sequence

MCSRIGEFNLPRNLVLCYESGTQTTDPVANSFQLGANVACQKAHVQYKVTSNSTVEIKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLFATLHALWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVGIRWSSIFYVESIARVQRLSLSGLILYKLYMADQFFVQWPQLQRKYPRAHYVGCLM

Homology

[Close]

BLAST of Lag0017289 vs. NCBI nr
Match: XP_038904772.1 (UDP-N-acetylglucosamine transferase subunit ALG14 homolog [Benincasa hispida])

HSP 1 Score: 253.8 bits (647), Expect = 1.1e-63
Identity = 127/149 (85.23%), Postives = 135/149 (90.60%), Query Frame = 0


Query: 37  NVACQKAHVQYKVTSNSTVEIKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLFATLHA 96
           N++ QKA      T  + +  KTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTL ATLHA
Sbjct: 79  NMSLQKAR-----TYENQLADKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLIATLHA 138

Query: 97  LWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVGIRWSSIFYVESIARVQRLSLSGLILY 156
           LWLM+KI+PQVILCNGPGTCIPLCVIAFIFKV+GIRWSSIFYVESIARV+RLSLSGLILY
Sbjct: 139 LWLMLKIKPQVILCNGPGTCIPLCVIAFIFKVLGIRWSSIFYVESIARVKRLSLSGLILY 198

Query: 157 KLYMADQFFVQWPQLQRKYPRAHYVGCLM 186
           KLYMADQFFVQWPQLQR YPRAHYVGCLM
Sbjct: 199 KLYMADQFFVQWPQLQRIYPRAHYVGCLM 222

[Close]

BLAST of Lag0017289 vs. NCBI nr
Match: XP_023552178.1 (UDP-N-acetylglucosamine transferase subunit ALG14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.5 bits (641), Expect = 5.5e-63
Identity = 126/149 (84.56%), Postives = 134/149 (89.93%), Query Frame = 0


Query: 37  NVACQKAHVQYKVTSNSTVEIKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLFATLHA 96
           N++ QKA      T  + +  KTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTL ATLHA
Sbjct: 80  NMSLQKAR-----TYENQLADKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLIATLHA 139

Query: 97  LWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVGIRWSSIFYVESIARVQRLSLSGLILY 156
           LWLM+K+RPQVILCNGPGTCIPLCVIAFIFKV+GIRWSSIFYVESIARV+RLSLSGLILY
Sbjct: 140 LWLMLKLRPQVILCNGPGTCIPLCVIAFIFKVLGIRWSSIFYVESIARVKRLSLSGLILY 199

Query: 157 KLYMADQFFVQWPQLQRKYPRAHYVGCLM 186
           KLYMADQFFVQWPQLQR YPRA YVGCLM
Sbjct: 200 KLYMADQFFVQWPQLQRLYPRARYVGCLM 223

[Close]

BLAST of Lag0017289 vs. NCBI nr
Match: TYK11484.1 (UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo var. makuwa])

HSP 1 Score: 250.4 bits (638), Expect = 1.2e-62
Identity = 126/149 (84.56%), Postives = 134/149 (89.93%), Query Frame = 0


Query: 37  NVACQKAHVQYKVTSNSTVEIKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLFATLHA 96
           N++ QKA      T  + +  KT AEVDKIAQFMQIYRSREVGQSYFTSVWTT+ ATLHA
Sbjct: 98  NMSLQKAR-----TYENQLADKTEAEVDKIAQFMQIYRSREVGQSYFTSVWTTVIATLHA 157

Query: 97  LWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVGIRWSSIFYVESIARVQRLSLSGLILY 156
           LWLMIKIRPQVILCNGPGTCIPLC+IAFIFKV+GIRWSSIFYVESIARV+RLSLSGLILY
Sbjct: 158 LWLMIKIRPQVILCNGPGTCIPLCLIAFIFKVLGIRWSSIFYVESIARVKRLSLSGLILY 217

Query: 157 KLYMADQFFVQWPQLQRKYPRAHYVGCLM 186
           KLYMADQFFVQWPQLQR YPRAHYVGCLM
Sbjct: 218 KLYMADQFFVQWPQLQRIYPRAHYVGCLM 241

[Close]

BLAST of Lag0017289 vs. NCBI nr
Match: XP_008448662.1 (PREDICTED: UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo] >XP_008448663.1 PREDICTED: UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo] >XP_008448665.1 PREDICTED: UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo] >XP_008448666.1 PREDICTED: UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo])

HSP 1 Score: 250.4 bits (638), Expect = 1.2e-62
Identity = 126/149 (84.56%), Postives = 134/149 (89.93%), Query Frame = 0


Query: 37  NVACQKAHVQYKVTSNSTVEIKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLFATLHA 96
           N++ QKA      T  + +  KT AEVDKIAQFMQIYRSREVGQSYFTSVWTT+ ATLHA
Sbjct: 79  NMSLQKAR-----TYENQLADKTEAEVDKIAQFMQIYRSREVGQSYFTSVWTTVIATLHA 138

Query: 97  LWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVGIRWSSIFYVESIARVQRLSLSGLILY 156
           LWLMIKIRPQVILCNGPGTCIPLC+IAFIFKV+GIRWSSIFYVESIARV+RLSLSGLILY
Sbjct: 139 LWLMIKIRPQVILCNGPGTCIPLCLIAFIFKVLGIRWSSIFYVESIARVKRLSLSGLILY 198

Query: 157 KLYMADQFFVQWPQLQRKYPRAHYVGCLM 186
           KLYMADQFFVQWPQLQR YPRAHYVGCLM
Sbjct: 199 KLYMADQFFVQWPQLQRIYPRAHYVGCLM 222

[Close]

BLAST of Lag0017289 vs. NCBI nr
Match: KAA0053029.1 (UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo var. makuwa])

HSP 1 Score: 250.4 bits (638), Expect = 1.2e-62
Identity = 126/149 (84.56%), Postives = 134/149 (89.93%), Query Frame = 0


Query: 37  NVACQKAHVQYKVTSNSTVEIKTGAEVDKIAQFMQIYRSREVGQSYFTSVWTTLFATLHA 96
           N++ QKA      T  + +  KT AEVDKIAQFMQIYRSREVGQSYFTSVWTT+ ATLHA
Sbjct: 89  NMSLQKAR-----TYENQLADKTEAEVDKIAQFMQIYRSREVGQSYFTSVWTTVIATLHA 148

Query: 97  LWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVGIRWSSIFYVESIARVQRLSLSGLILY 156
           LWLMIKIRPQVILCNGPGTCIPLC+IAFIFKV+GIRWSSIFYVESIARV+RLSLSGLILY
Sbjct: 149 LWLMIKIRPQVILCNGPGTCIPLCLIAFIFKVLGIRWSSIFYVESIARVKRLSLSGLILY 208

Query: 157 KLYMADQFFVQWPQLQRKYPRAHYVGCLM 186
           KLYMADQFFVQWPQLQR YPRAHYVGCLM
Sbjct: 209 KLYMADQFFVQWPQLQRIYPRAHYVGCLM 232

[Close]

BLAST of Lag0017289 vs. ExPASy Swiss-Prot
Match: Q6AY85 (UDP-N-acetylglucosamine transferase subunit ALG14 homolog OS=Rattus norvegicus OX=10116 GN=Alg14 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-25
Identity = 57/112 (50.89%), Postives = 84/112 (75.00%), Query Frame = 0


Query: 71  QIYRSREVGQSYFTSVWTTLFATLHALWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVG 130
           +I RSREV QS+ +SV+TTL++   +  L+ +I+P ++LCNGPGTC+P+CV A +  ++G
Sbjct: 104 RIPRSREVRQSWLSSVFTTLYSIWFSFPLVHRIKPDLVLCNGPGTCVPICVSALLLGILG 163

Query: 131 IRWSSIFYVESIARVQRLSLSGLILYKLYMADQFFVQWPQLQRKYPRAHYVG 183
           I+   I YVESI RV+ LSLSG IL+  +++D F VQWP L+ KYP++ Y+G
Sbjct: 164 IKKVIIVYVESICRVETLSLSGKILW--HLSDYFIVQWPTLKEKYPKSVYLG 213

[Close]

BLAST of Lag0017289 vs. ExPASy Swiss-Prot
Match: Q9D081 (UDP-N-acetylglucosamine transferase subunit ALG14 homolog OS=Mus musculus OX=10090 GN=Alg14 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 6.2e-25
Identity = 55/112 (49.11%), Postives = 83/112 (74.11%), Query Frame = 0


Query: 71  QIYRSREVGQSYFTSVWTTLFATLHALWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVG 130
           +I RSREV QS+ +SV+TT ++   +  L+++I+P ++LCNGPGTC+P+CV A +  ++G
Sbjct: 105 RIPRSREVRQSWLSSVFTTFYSMWFSFPLVLRIKPDLVLCNGPGTCVPICVSALLLGILG 164

Query: 131 IRWSSIFYVESIARVQRLSLSGLILYKLYMADQFFVQWPQLQRKYPRAHYVG 183
           ++   I YVESI RV+ LSLSG IL   +++D F VQWP L+ KYP++ Y+G
Sbjct: 165 VKKVIIVYVESICRVETLSLSGKIL--RHLSDYFIVQWPTLKEKYPKSVYLG 214

[Close]

BLAST of Lag0017289 vs. ExPASy Swiss-Prot
Match: Q5A5N6 (UDP-N-acetylglucosamine transferase subunit ALG14 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ALG14 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 57/119 (47.90%), Postives = 86/119 (72.27%), Query Frame = 0


Query: 67  AQFMQIYRSREVGQSYFTSVWTTLFATLHALWLMIKIRPQVILCNGPGTCIPLCVIAFIF 126
           +Q++ I R+R VGQSY +S+ TT+++ L +   M+K RP VIL NGPGTC+P+  I F++
Sbjct: 103 SQYIPIPRARTVGQSYISSIPTTIYSFLFSAIAMLKHRPAVILLNGPGTCVPVAYILFLY 162

Query: 127 KVVGIRWSSIFYVESIARVQRLSLSGLILYKLYMADQFFVQWPQLQRKYPRAHYVGCLM 186
           K++G+  + I Y+ES+ARV +LSLSGL+L  L ++D+F VQW  L ++Y R  Y G L+
Sbjct: 163 KLLGLCNTKIIYIESLARVNKLSLSGLLL--LPISDRFIVQWESLYQQYSRVEYYGILI 219

[Close]

BLAST of Lag0017289 vs. ExPASy Swiss-Prot
Match: Q96F25 (UDP-N-acetylglucosamine transferase subunit ALG14 homolog OS=Homo sapiens OX=9606 GN=ALG14 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 5.3e-24
Identity = 56/116 (48.28%), Postives = 83/116 (71.55%), Query Frame = 0


Query: 71  QIYRSREVGQSYFTSVWTTLFATLHALWLMI----KIRPQVILCNGPGTCIPLCVIAFIF 130
           +I RSREV QS+     +T+F TLH++WL      +++P ++LCNGPGTC+P+CV A + 
Sbjct: 104 RIPRSREVQQSW----PSTVFTTLHSMWLSFPLIHRVKPDLVLCNGPGTCVPICVSALLL 163

Query: 131 KVVGIRWSSIFYVESIARVQRLSLSGLILYKLYMADQFFVQWPQLQRKYPRAHYVG 183
            ++GI+   I YVESI RV+ LS+SG IL+  +++D F VQWP L+ KYP++ Y+G
Sbjct: 164 GILGIKKVIIVYVESICRVETLSMSGKILF--HLSDYFIVQWPALKEKYPKSVYLG 213

[Close]

BLAST of Lag0017289 vs. ExPASy Swiss-Prot
Match: Q6CF02 (UDP-N-acetylglucosamine transferase subunit ALG14 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ALG14 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-23
Identity = 61/114 (53.51%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 72  IYRSREVGQSYFTSVWTTLFATLHALWLMIKIRPQVILCNGPGTCIPLCVIAFIFKVVGI 131
           I R+R+VGQSY +SV T+  +   A+ L+ K +P VI+CNGP TC+ LC  AF+ + + +
Sbjct: 105 IPRARKVGQSYPSSVITSAVSFAVAVKLVHKHKPHVIVCNGPATCVMLCYAAFLLRFMAL 164

Query: 132 RWSSIFYVESIARVQRLSLSGLILYKLYMADQFFVQWPQLQRKYPRAHYVGCLM 186
             + I YVES+ARV RLSLSGLIL  L   D+F VQWPQL  KYPRA Y G L+
Sbjct: 165 IDTRIIYVESLARVNRLSLSGLIL--LPFCDRFLVQWPQLAEKYPRAEYHGILV 216

The following BLAST results are available for this feature:

BLAST of Lag0017289 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904772.1	1.1e-63	85.23	UDP-N-acetylglucosamine transferase subunit ALG14 homolog [Benincasa hispida]	[more]
XP_023552178.1	5.5e-63	84.56	UDP-N-acetylglucosamine transferase subunit ALG14-like [Cucurbita pepo subsp. pe...	[more]
TYK11484.1	1.2e-62	84.56	UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo var. makuwa]	[more]
XP_008448662.1	1.2e-62	84.56	PREDICTED: UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo] >XP_...	[more]
KAA0053029.1	1.2e-62	84.56	UDP-N-acetylglucosamine transferase subunit ALG14 [Cucumis melo var. makuwa]	[more]

BLAST of Lag0017289 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AY85	2.8e-25	50.89	UDP-N-acetylglucosamine transferase subunit ALG14 homolog OS=Rattus norvegicus O...	[more]
Q9D081	6.2e-25	49.11	UDP-N-acetylglucosamine transferase subunit ALG14 homolog OS=Mus musculus OX=100...	[more]
Q5A5N6	1.1e-24	47.90	UDP-N-acetylglucosamine transferase subunit ALG14 OS=Candida albicans (strain SC...	[more]
Q96F25	5.3e-24	48.28	UDP-N-acetylglucosamine transferase subunit ALG14 homolog OS=Homo sapiens OX=960...	[more]
Q6CF02	1.2e-23	53.51	UDP-N-acetylglucosamine transferase subunit ALG14 OS=Yarrowia lipolytica (strain...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013969	Oligosaccharide biosynthesis protein Alg14-like	PFAM	PF08660	Alg14	coord: 59..184 e-value: 2.7E-48 score: 164.2
IPR013969	Oligosaccharide biosynthesis protein Alg14-like	PANTHER	PTHR12154	GLYCOSYL TRANSFERASE-RELATED	coord: 51..185
None	No IPR available	GENE3D	3.40.50.2000	Glycogen Phosphorylase B;	coord: 36..185 e-value: 2.6E-17 score: 64.8

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017289.1	Lag0017289.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006488	dolichol-linked oligosaccharide biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031965	nuclear membrane
cellular_component	GO:0043541	UDP-N-acetylglucosamine transferase complex
molecular_function	GO:0016740	transferase activity