Lag0017219 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017219
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	chr5: 1116349 .. 1117864 (+)
RNA-Seq Expression	Lag0017219
Synteny	Lag0017219

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGAAATCTTCAAGTACCTCGTCGGTTCCGCCGGAGCAAGCGGCTATGGCTCCAAATCCACCGCCGACGAGGTCACTCAAGCATGTTCCCATCTGCACTCCGTCACCGCCATAATCACAGGTGATTGTCTTTGAATTCTGAATTCAGATTTTCGATTGTATCATATTGATAAATTGAATTCTCTCGATAACAAATTTTGGCGCAGGAGCGACGTCCGGAATCGGAGAGGAGACGGCGAGAGTATTAGCCAAGCGTGGCGCCAGGATCGTCATTCCGGCTCGCGACCTCAAAGCCGCGGGGGAAGCGAAGGCGCGAATCGTGTCGGAATCGGAATGTATCGATCATTCACGAATCACGGTGATGGCTCTCGATCTTAGTTCTCTGAATTCCGTCACGAACTTCGTCTCCGAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATGTAATTCATCCAAAACATCGCTAATTCACTCCGTTGAATCGAACGAACAAGCGACATTAATTGATTTCGAAGAAACTAAAAGCCGATTTCGTTATTTTTTATCAGAAACAATGCCGGTAGGTTCTGTTACGAACATGCGATTTCTGAAGACGGAATTGAGATGACGTTCGCTACTAATTATCTAGGTAAGTACGTAGAACAAAATTTATGTTCATCTCTAATTCATGAAGCCTTTTACCATTATTGATCGAATCGAGCATGCAGGTCATTTCCTGTTGACGAAACTTTTGCTGAATAAGATGATCGCAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTCTCCGGCGACATATTCGAATATCTCGACCAGATAAGCTCGAACAACAGGTGTGATTAATTTTCCTTTACTTACGTATTAATGGCGGTCTGATAGCGATACCGATTTTCACTCGCAGCAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCCAATGTCTTGCACACCATCCAACTCGCTCACAGACTCCAGGTTATCAATTGGTATTAATTTTCATAGAATGATTTCAAGAACGAGTTGGTTTTTGACAAAATCAAAACAGGAAATGGAGGCAAATGTGACGGTAAACTGCGTTCATCCGGGCGTAGTGAAGACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGGTATTGATCAAAGCTCCTGCATGGAAATTAATCAATCTAAAACGTATTAAGTCTATATAATTAATTAATTTGATGAGAAAACACAGATTTGATTTTCTACATGGCGTCCAAATCAAAGTTGTTGAAGACAATTCCTCAAGGAGCAGCAACAAGTTGTTACGTAGCCGCAAATCAGAAAGTGGAAAATGTGAATGGAAAATACTTTGAGGATTGCAACGAACAAGAAAGCTGCGAATCCAAATTAGGTACCAAATCCACTCACTTGGCTGCACGCCTATGGTCCGCCTCTGAAACCATCGTCTCCACTCTTACAGGTAACCCTGAAGAACTCTAA

mRNA sequence

ATGCTTGAAATCTTCAAGTACCTCGTCGGTTCCGCCGGAGCAAGCGGCTATGGCTCCAAATCCACCGCCGACGAGGTCACTCAAGCATGTTCCCATCTGCACTCCGTCACCGCCATAATCACAGGAGCGACGTCCGGAATCGGAGAGGAGACGGCGAGAGTATTAGCCAAGCGTGGCGCCAGGATCGTCATTCCGGCTCGCGACCTCAAAGCCGCGGGGGAAGCGAAGGCGCGAATCGTGTCGGAATCGGAATGTATCGATCATTCACGAATCACGGTGATGGCTCTCGATCTTAGTTCTCTGAATTCCGTCACGAACTTCGTCTCCGAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATAAACAATGCCGGTAGGTTCTGTTACGAACATGCGATTTCTGAAGACGGAATTGAGATGACGTTCGCTACTAATTATCTAGGTCATTTCCTGTTGACGAAACTTTTGCTGAATAAGATGATCGCAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTCTCCGGCGACATATTCGAATATCTCGACCAGATAAGCTCGAACAACAGCAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCCAATGTCTTGCACACCATCCAACTCGCTCACAGACTCCAGGAAATGGAGGCAAATGTGACGGTAAACTGCGTTCATCCGGGCGTAGTGAAGACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAAGTTGTTGAAGACAATTCCTCAAGGAGCAGCAACAAGTTGTTACGTAGCCGCAAATCAGAAAGTGGAAAATGTGAATGGAAAATACTTTGAGGATTGCAACGAACAAGAAAGCTGCGAATCCAAATTAGGTACCAAATCCACTCACTTGGCTGCACGCCTATGGTCCGCCTCTGAAACCATCGTCTCCACTCTTACAGGTAACCCTGAAGAACTCTAA

Coding sequence (CDS)

ATGCTTGAAATCTTCAAGTACCTCGTCGGTTCCGCCGGAGCAAGCGGCTATGGCTCCAAATCCACCGCCGACGAGGTCACTCAAGCATGTTCCCATCTGCACTCCGTCACCGCCATAATCACAGGAGCGACGTCCGGAATCGGAGAGGAGACGGCGAGAGTATTAGCCAAGCGTGGCGCCAGGATCGTCATTCCGGCTCGCGACCTCAAAGCCGCGGGGGAAGCGAAGGCGCGAATCGTGTCGGAATCGGAATGTATCGATCATTCACGAATCACGGTGATGGCTCTCGATCTTAGTTCTCTGAATTCCGTCACGAACTTCGTCTCCGAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATAAACAATGCCGGTAGGTTCTGTTACGAACATGCGATTTCTGAAGACGGAATTGAGATGACGTTCGCTACTAATTATCTAGGTCATTTCCTGTTGACGAAACTTTTGCTGAATAAGATGATCGCAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTCTCCGGCGACATATTCGAATATCTCGACCAGATAAGCTCGAACAACAGCAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCCAATGTCTTGCACACCATCCAACTCGCTCACAGACTCCAGGAAATGGAGGCAAATGTGACGGTAAACTGCGTTCATCCGGGCGTAGTGAAGACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAAGTTGTTGAAGACAATTCCTCAAGGAGCAGCAACAAGTTGTTACGTAGCCGCAAATCAGAAAGTGGAAAATGTGAATGGAAAATACTTTGAGGATTGCAACGAACAAGAAAGCTGCGAATCCAAATTAGGTACCAAATCCACTCACTTGGCTGCACGCCTATGGTCCGCCTCTGAAACCATCGTCTCCACTCTTACAGGTAACCCTGAAGAACTCTAA

Protein sequence

MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNIHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFEDCNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPEEL

Homology

[Close]

BLAST of Lag0017219 vs. NCBI nr
Match: KAA0052978.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 565.8 bits (1457), Expect = 2.4e-157
Identity = 292/342 (85.38%), Postives = 315/342 (92.11%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAA EAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMI TAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS----SNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVT 240
           IHSWFSGDIFEYL QIS    +NNSREYDATRAY+FSKLAN+LHT+QLAHRLQEM+ANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGK 300
           VNCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA ++KVENVNGK
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFEDCNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           YFEDCNEQ SCES   TKS HLA RLWSASE I+S LTGNP+
Sbjct: 301 YFEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 340

[Close]

BLAST of Lag0017219 vs. NCBI nr
Match: XP_008449162.1 (PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo])

HSP 1 Score: 565.1 bits (1455), Expect = 4.2e-157
Identity = 291/341 (85.34%), Postives = 315/341 (92.38%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAA EAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMI TAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS---SNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTV 240
           IHSWFSGDIFEYL QIS   +NN+REYDATRAY+FSKLAN+LHT+QLAHRLQEM+ANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKY 300
           NCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA ++KVENVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FEDCNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           FEDCNEQ SCES   TKS HLA RLWSASE I+S LTGNP+
Sbjct: 301 FEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 339

[Close]

BLAST of Lag0017219 vs. NCBI nr
Match: XP_011650304.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >KAE8650011.1 hypothetical protein Csa_011335 [Cucumis sativus])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 291/340 (85.59%), Postives = 312/340 (91.76%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGSKSTA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAK GA
Sbjct: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKGGA 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAA EAKARIVSE EC DHSRITVM LDLSSLNSV NFVSEFESL+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDHSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMI TA+STGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAESTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNS--REYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVN 240
           IHSWFSGD+FEYL QIS NN+  REYDATRAYAFSKLAN+LHT+QLAHRLQEM+ANVTVN
Sbjct: 181 IHSWFSGDVFEYLSQISRNNNKYREYDATRAYAFSKLANLLHTVQLAHRLQEMKANVTVN 240

Query: 241 CVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYF 300
           CVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA ++KVENVNGKYF
Sbjct: 241 CVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKYF 300

Query: 301 EDCNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
            DCNEQ SCES   TKS HLA RLWS SE IVS LTG P+
Sbjct: 301 ADCNEQGSCES--NTKSAHLAERLWSTSEIIVSNLTGKPQ 338

[Close]

BLAST of Lag0017219 vs. NCBI nr
Match: XP_022923250.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6577763.1 Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 561.6 bits (1446), Expect = 4.6e-156
Identity = 286/338 (84.62%), Postives = 312/338 (92.31%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAA EAKARIVSES+C DHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MI TAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLHT+QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFED 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLKTIP+GAATSCYVA N+ VENVNG YF D
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVSTLTG P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Lag0017219 vs. NCBI nr
Match: XP_023552711.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.9 bits (1439), Expect = 3.0e-155
Identity = 284/338 (84.02%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAA EAKARIVSES+C DHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MI TAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLHT+QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFED 300
           HPGVVKT LNR+REGFVTDLIFYM SKS+LLKTIP+GAAT+CYVA N+ VENVNG YF D
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVSTLTG P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Lag0017219 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 153/334 (45.81%), Postives = 213/334 (63.77%), Query Frame = 0


Query: 2   LEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGAR 61
           +EI+  + G AG SGYGS STA++VT +    H +TAIITG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKH-LTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLL 121
           ++I AR+ KAA ++K  I+   +   ++RI  + LDLSS+ SV +F+ +F +L++PLN+L
Sbjct: 61  VIIAARNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNI 181
           INNAG       +SEDGIE  FATN++GHFLLT LLL+KM ++A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNC 241
           H  ++  G +F+Y+     N+   Y   +AY  SKLAN+LH+  L+ +LQE   N+T+N 
Sbjct: 181 HTYTYTEGIMFDYI-----NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINS 240

Query: 242 VHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFE 301
           VHPG++ T L R        +    A    L K IPQGAAT+CYVA +  ++ V GKYF 
Sbjct: 241 VHPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFT 300

Query: 302 DCNEQESCESKLGTKSTHLAARLWSASETIVSTL 334
           DCN        +    T LA +LW  S  +V +L
Sbjct: 301 DCNVTTPSNFAI---DTTLADKLWDFSIKLVDSL 319

[Close]

BLAST of Lag0017219 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.9e-67
Identity = 152/334 (45.51%), Postives = 214/334 (64.07%), Query Frame = 0


Query: 2   LEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGAR 61
           +EI+  + G AG SGYGS STA++VT +    H +TAIITG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKH-LTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLL 121
           ++I +R+ KAA ++K  I+   +   ++RI  + LDLSS+ SV +F+ +F +L++PLN+L
Sbjct: 61  VIIASRNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNI 181
           INNAG       +SEDGIE  FATN++GHFLLT LLL+KM ++A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNC 241
           H  ++  G +F+Y+     N+   Y   +AY  SKLAN+LH+  L+ +LQE   N+T+N 
Sbjct: 181 HTYTYTEGIMFDYI-----NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINS 240

Query: 242 VHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFE 301
           VHPG++ T L R        +    A    L K IPQGAAT+CYVA +  +++V GKYF 
Sbjct: 241 VHPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFA 300

Query: 302 DCNEQESCESKLGTKSTHLAARLWSASETIVSTL 334
           DCN        +    T LA +LW  S  +V +L
Sbjct: 301 DCNVTTPSNFAI---DTTLADKLWDFSIKLVESL 319

[Close]

BLAST of Lag0017219 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 9.0e-62
Identity = 144/329 (43.77%), Postives = 204/329 (62.01%), Query Frame = 0


Query: 7   YLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIVIPA 66
           +  GS GASG+ S+STA+EVT        +TAI+TGA+SGIG ETARVL+ RG  +V+  
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTHGVDGT-GLTAIVTGASSGIGVETARVLSLRGVHVVMAV 61

Query: 67  RDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAG 126
           R+  +  + K  IV +   +  +++ VM LDLSS+ SV  F SE++S  LPLNLLINNAG
Sbjct: 62  RNTDSGAKVKEDIVKQ---VPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAG 121

Query: 127 RFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNIHSWFS 186
                  +S+D IE+ FATN+LGHFLLTKLLL+ M +T++ +  +GRIVN++S  H  FS
Sbjct: 122 IMACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHR-FS 181

Query: 187 GDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCVHPGVVK 246
                  D+I  N+   Y + RAY  SKL NVLH  +L  +L+E   N+T N +HPG + 
Sbjct: 182 YPEGVRFDKI--NDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIM 241

Query: 247 TKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFEDCNEQES 306
           T L R    ++   +  +A    +LK++PQGAAT+CYVA N +V  V+G+YF+D N  + 
Sbjct: 242 TNLGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKP 301

Query: 307 CESKLGTKSTHLAARLWSASETIVSTLTG 336
                  K T LA ++W  S  +  + +G
Sbjct: 302 LPL---VKDTELAKKVWDFSTKLTDSQSG 318

[Close]

BLAST of Lag0017219 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 9.6e-56
Identity = 138/318 (43.40%), Postives = 190/318 (59.75%), Query Frame = 0


Query: 13  GASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIVIPARDLKAA 72
           G SG+   STA++VT        +TAI+TGA+SGIG ET RVLA RGA +++  R++ AA
Sbjct: 9   GVSGFSGSSTAEQVTHGID-ATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  GEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAGRFCYEH 132
            + K  I+ +   I  +++  + LDLSSL+SV  F SEF S   PLN+LINNAG      
Sbjct: 69  KDVKDTILKD---IPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPF 128

Query: 133 AISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNIHSWFSGDIFEY 192
            +S+D IE+ FATN++GHFLLT LLL+ M  T + +  +GRIVNV S  H +   +   +
Sbjct: 129 KLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF 188

Query: 193 LDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCVHPGVVKTKLNRD 252
            D+I  N+   Y+  RAY  SKLANVLH  QL   L+E   N+T N +HPG + T L R 
Sbjct: 189 -DKI--NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRH 248

Query: 253 REGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFEDCNEQESCESKLG 312
               V  LI  +     +LK + QGAAT+CYVA + +V+ V+G+YF D N  ++      
Sbjct: 249 NSA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPH--- 308

Query: 313 TKSTHLAARLWSASETIV 331
            K   LA +LW  S  +V
Sbjct: 309 GKDVDLAKKLWDFSINLV 313

[Close]

BLAST of Lag0017219 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.3e-41
Identity = 122/313 (38.98%), Postives = 176/313 (56.23%), Query Frame = 0


Query: 33  LHSVTAIITGATSGIGEETARVLAKRGARIVIPARDL----KAAGEAKARIVSESECIDH 92
           +H  T +ITGA SG+G  TA  L + GAR+++  RD     +AAG+ +  +   +EC   
Sbjct: 41  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPE 100

Query: 93  ------SRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAGRFCYEHAISEDGIEMT 152
                   + V  LDL+SL SV  F  E       L++LINNAG F   +  +EDG EM 
Sbjct: 101 PGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQ 160

Query: 153 FATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNIHSWFSGDI-FEYLDQISSNNS 212
           F  N+LGHFLLT LLL  + ++A S     RIV V+S ++ +  GDI F+ L     N+ 
Sbjct: 161 FGVNHLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GDINFDDL-----NSE 220

Query: 213 REYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCVHPGVVKTKLNRD-REGFVTDL 272
           + Y+ +  Y+ SKLAN+L T +LA RL+    NVTVN +HPG+V+T L R      +   
Sbjct: 221 QSYNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKP 280

Query: 273 IFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFEDCNEQESCESKLGTKSTHLAA 332
           +F + S +   KT  +GA TS Y+A++ +VE V+G+YF DC E+E     +      +A 
Sbjct: 281 LFNLVSWA-FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAM---DESVAR 335

Query: 333 RLWSASETIVSTL 334
           +LW  SE +V  L
Sbjct: 341 KLWDISEVMVGLL 335

[Close]

BLAST of Lag0017219 vs. ExPASy TrEMBL
Match: A0A5A7UCR1 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00880 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.2e-157
Identity = 292/342 (85.38%), Postives = 315/342 (92.11%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAA EAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMI TAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS----SNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVT 240
           IHSWFSGDIFEYL QIS    +NNSREYDATRAY+FSKLAN+LHT+QLAHRLQEM+ANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGK 300
           VNCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA ++KVENVNGK
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFEDCNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           YFEDCNEQ SCES   TKS HLA RLWSASE I+S LTGNP+
Sbjct: 301 YFEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 340

[Close]

BLAST of Lag0017219 vs. ExPASy TrEMBL
Match: A0A1S3BLF4 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491110 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 291/341 (85.34%), Postives = 315/341 (92.38%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAA EAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMI TAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS---SNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTV 240
           IHSWFSGDIFEYL QIS   +NN+REYDATRAY+FSKLAN+LHT+QLAHRLQEM+ANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKY 300
           NCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA ++KVENVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FEDCNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           FEDCNEQ SCES   TKS HLA RLWSASE I+S LTGNP+
Sbjct: 301 FEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 339

[Close]

BLAST of Lag0017219 vs. ExPASy TrEMBL
Match: A0A6J1EB90 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.2e-156
Identity = 286/338 (84.62%), Postives = 312/338 (92.31%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAA EAKARIVSES+C DHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MI TAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLHT+QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFED 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLKTIP+GAATSCYVA N+ VENVNG YF D
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVSTLTG P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Lag0017219 vs. ExPASy TrEMBL
Match: A0A6J1HN85 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.2e-154
Identity = 284/338 (84.02%), Postives = 310/338 (91.72%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAA EAKARIVSES C D SRITVMALDLSS+ SV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA+SEDGIEMTFATNYLGHFLLTKLLLN+MI TAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLHT+QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFED 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLK IP+GAATSCYVA N+ VENVNGKYF D
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVSTLTG P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Lag0017219 vs. ExPASy TrEMBL
Match: A0A6J1E6B3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.6e-154
Identity = 285/338 (84.32%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAA EAKARIVSES+C DHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MI TAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  N R YD+TRAYAFSKLANVLHT+QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFED 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLKTIP+GAATSCYVA N+ VENVNG YF D
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTLTGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVSTLTG P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 333

[Close]

BLAST of Lag0017219 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 4.1e-118
Identity = 226/331 (68.28%), Postives = 271/331 (81.87%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           M+E  KYL+G+AGASG+GSKSTA+EVT+ C  L S+TA+ITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCD-LRSITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           R++ PAR++KAA EAK RIVSE      + I VM LDLSS+ SV NFV++FESL LPLNL
Sbjct: 61  RLIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAG+  +EHAISEDGIEMTFATNYLGHFLLT LLLNKMI TA+ TG+QGRIVNVTS 
Sbjct: 121 LINNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSG 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCV 240
           IH WFSGD+ EYL  IS     ++DATRAYA SKLANVLHT +L+ RLQ++ ANVTVNCV
Sbjct: 181 IHGWFSGDLIEYLRLISQPKC-QFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFED 300
           HPGVV+T+L RDREG +TDL+F++A  SKL+KT+PQ AAT+CYVA N ++ NV+GKYF D
Sbjct: 241 HPGVVRTRLTRDREGLLTDLVFFLA--SKLVKTVPQAAATTCYVATNPRLVNVSGKYFTD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVS 332
           CN  E+  S LGT S+  A +LW+ASE +V+
Sbjct: 301 CN--ETTPSGLGTNSSE-ATKLWAASEILVT 321

[Close]

BLAST of Lag0017219 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 409.5 bits (1051), Expect = 2.7e-114
Identity = 219/331 (66.16%), Postives = 264/331 (79.76%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           M+E  K+L+GS G SG+GS+STAD VT   S L S+TAIITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVT-CNSDLRSLTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           R+V+PAR +K A E KARI+SE      + I VM LDLSSL SV  FV +FESL+LPLN+
Sbjct: 61  RLVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNI 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSN 180
           LINNAG++ ++HA+SEDG+EMTFATNYLGHFLLTKLLL KMI TA  TG+QGRIVNVTS 
Sbjct: 121 LINNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSV 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCV 240
           +HSWFSGD+ +YL  IS NN R YDATRAYA SKLANVLHT++L+  L +M+ANVT NCV
Sbjct: 181 VHSWFSGDMLQYLADISRNN-RNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFED 300
           HPG+VKT+L RDREG VTDL+F++   SKLLK++PQ AAT+CYVA + ++ NV GKYF D
Sbjct: 241 HPGIVKTRLTRDREGVVTDLVFFLT--SKLLKSVPQAAATTCYVATSPRLRNVCGKYFSD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVS 332
           CNE  S  SK G+ +   A RLW+AS+ +VS
Sbjct: 301 CNEARS--SKSGSCNLK-AQRLWTASDLLVS 321

[Close]

BLAST of Lag0017219 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 3.1e-78
Identity = 167/332 (50.30%), Postives = 224/332 (67.47%), Query Frame = 0


Query: 6   KYLVGSAGASGYGSKSTADEVTQ----ACSHLHSVTAIITGATSGIGEETARVLAKRGAR 65
           +YL G AG +G+GS+STA++VTQ     CSHL   TAIITG TSGIG ETARVLAKRG R
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHL---TAIITGGTSGIGAETARVLAKRGVR 65

Query: 66  IVIPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLL 125
           +V+  RD+K A   K RI+ E+   D   I +  +DLSSL+SV  F S+F S  LPLN+L
Sbjct: 66  VVMAVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNIL 125

Query: 126 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNI 185
           INNAG F      SE+ IE+TFATN+LGH+LLT++L+ KMI TA+ +GI+GRI+N++S I
Sbjct: 126 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 185

Query: 186 HSWFSGDIFEY---LDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVN 245
           H+W   D F +   L  IS      Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N
Sbjct: 186 HNWVKPDCFSFPKLLHPIS-----RYNGTRAYAQSKLATILHAKALSKQLKDRNANVTIN 245

Query: 246 CVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYF 305
            VHPG+VKT + R  +G  TD +F +A  SKLLK+I QGAAT+CYVA + + + ++GKYF
Sbjct: 246 AVHPGIVKTGIIRAHKGLFTDSLFLIA--SKLLKSISQGAATTCYVALSNETKGLSGKYF 305

Query: 306 EDCNEQESCESKLGTKSTHLAARLWSASETIV 331
            DCNE    +        ++A +L + S  ++
Sbjct: 306 ADCNETNCSDL---ANDEYVALKLCTQSRALI 321

[Close]

BLAST of Lag0017219 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.4e-72
Identity = 160/333 (48.05%), Postives = 221/333 (66.37%), Query Frame = 0


Query: 4   IFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIV 63
           ++  + G  G SG+GS STA+EVTQ      ++TAIITG T GIG ETARVL+KRGA +V
Sbjct: 3   LYSLITGRRGPSGFGSASTAEEVTQGID-ATNLTAIITGGTGGIGMETARVLSKRGAHVV 62

Query: 64  IPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLIN 123
           I AR++ AA  AK  I+ ++    ++R+T++ LDLSS+ S+  FV EF +LHLPLNLLIN
Sbjct: 63  IGARNMGAAENAKTEILRQNA---NARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLIN 122

Query: 124 NAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTS--NI 183
           NAG     + +SEDGIE+ FATN++GHFLLT LLL+ M  TAK++G++GRI+NV+S  +I
Sbjct: 123 NAGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHI 182

Query: 184 HSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCVH 243
           +++  G  F+ ++ I S     Y   RAY  SKLAN+LH  +L+ +LQE   N+T N VH
Sbjct: 183 YTYQEGIQFDSINDICS-----YSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVH 242

Query: 244 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFEDC 303
           PG++ T L +     +  L F+      L K IPQGAAT+CYVA +  V+ V GKYF DC
Sbjct: 243 PGLILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADC 302

Query: 304 NEQESCESKLGTKSTHLAARLWSASETIVSTLT 335
           N  E   SKL    T LA +LW  S  ++++++
Sbjct: 303 N--EVTPSKLARDET-LAQKLWDFSVKLINSVS 320

[Close]

BLAST of Lag0017219 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 3.8e-68
Identity = 153/331 (46.22%), Postives = 215/331 (64.95%), Query Frame = 0


Query: 4   IFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIV 63
           I+  + G  G SG+GS STA++VTQA    H +TAIITG TSGIG E ARVLA RGA ++
Sbjct: 3   IYGVMTGKKGKSGFGSASTAEDVTQAIDASH-LTAIITGGTSGIGLEAARVLAMRGAHVI 62

Query: 64  IPARDLKAAGEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLIN 123
           I AR+ KAA E+K  I+  +    ++R+  + +D+SS+ SV +FV +F +L++PLN+LIN
Sbjct: 63  IAARNPKAANESKEMILQMN---PNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILIN 122

Query: 124 NAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIATAKSTGIQGRIVNVTSNIHS 183
           NAG       ++EDGIE  FATN++GHFLLT LLL+KM +TA+ +G+QGRIVN++S  H+
Sbjct: 123 NAGVMFCPFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHT 182

Query: 184 WFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHTIQLAHRLQEMEANVTVNCVHPG 243
           +   +  ++      N+   Y   RAY  SKL+N+LH+  L+ RLQE   N+T+N VHPG
Sbjct: 183 YTYSEGIKFQ---GINDPAGYSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPG 242

Query: 244 VVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANQKVENVNGKYFEDCNE 303
           +V T L R   GF   +  + A      K IPQGAAT+CYVA +  +E V GKYF DCN 
Sbjct: 243 LVTTNLFR-YSGF--SMKVFRAMTFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCN- 302

Query: 304 QESCESKLGTKSTHLAARLWSASETIVSTLT 335
                SK  T ++ LA +LW  S  ++ +++
Sbjct: 303 -IVAPSKFATNNS-LADKLWDFSVFLIDSIS 320

The following BLAST results are available for this feature:

BLAST of Lag0017219 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0052978.1	2.4e-157	85.38	short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa]	[more]
XP_008449162.1	4.2e-157	85.34	PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo]	[more]
XP_011650304.1	1.2e-156	85.59	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >...	[more]
XP_022923250.1	4.6e-156	84.62	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch...	[more]
XP_023552711.1	3.0e-155	84.02	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo ...	[more]

BLAST of Lag0017219 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078ISJ6	1.1e-67	45.81	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078IS66	1.9e-67	45.51	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	9.0e-62	43.77	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	9.6e-56	43.40	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	2.3e-41	38.98	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of Lag0017219 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UCR1	1.2e-157	85.38	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3BLF4	2.0e-157	85.34	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1EB90	2.2e-156	84.62	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]
A0A6J1HN85	1.2e-154	84.02	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]
A0A6J1E6B3	1.6e-154	84.32	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos...	[more]

BLAST of Lag0017219 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	4.1e-118	68.28	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	2.7e-114	66.16	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	3.1e-78	50.30	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	4.4e-72	48.05	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G37540.1	3.8e-68	46.22	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 116..127 score: 42.55 coord: 210..229 score: 30.13 coord: 37..54 score: 44.95 coord: 233..250 score: 36.72 coord: 166..182 score: 27.32
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..180 e-value: 1.8E-25 score: 89.6
None	No IPR available	GENE3D	3.40.50.720		coord: 23..333 e-value: 3.2E-70 score: 238.5
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..334
None	No IPR available	PANTHER	PTHR24320:SF210	MITOCHONDRIAL PYRUVATE CARRIER	coord: 4..334
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 37..323 e-value: 1.20635E-89 score: 267.938
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..303

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017219.1	Lag0017219.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity