Lag0017210 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017210
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	methionine gamma-lyase-like
Location	chr5: 1010133 .. 1013564 (-)
RNA-Seq Expression	Lag0017210
Synteny	Lag0017210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAATTCAAAACCGGCACAAAAAGACCGACCTCCGATGAAACCGACGAGAATTTGATCTCGAAGAAACCAACGGCCATGATTTCGTCTCTCCTCGAGGATCCAGCGGCTGCGATCGCCAACACGCGTCACGAGTTCGGCGAGCACGGAGGGGTAAATATGTCAATCGAAGCCTCCGCCACATTCACCGTCATGGAACCGGAGACCATGCGGCGGATGTTCGCCGGCGAGCTCGGCCCCGACCGTGACTTCTTCATCTACAGCCGCCATTTCAATCCGACGGTGCTGAATCTCAGCCGCCAGATGGCCGCCCTCGAAGGCACCGCCGCCGCCTACTGCACCTCCAGCGGAATGTCGGCCATCGCCGCCGTGCTCCTCCAGCTCCTCGCCAGTGGGGACCACGTCGTGGCGTCGAGAACGCTCTACGGTGGGACCCATGCGCTGCTGTCGCATTTTTTGCCGAGGACGTCGAACATAACGACGTCGTTTGTGGACGTAAGTGATTTTGAGATGGTGAGGAGGGAGATCGTTGAAGGGAAAACGAAAGTGCTTTATTTCGAAGCGGTTTCGAATCCGACTTTGGCGGTGGCGGATATACCGGAGTTGTGCCGGATTGGACATGAAAAGGGGCTGACGGTGGTCGTCGATAATACTTTTGCGCCGATGGTTCTGTCGCCGGCGAGGCTCGGTGCTGACGTGGTTGTTCATAGTATTTCTAAGTACATTAGCGGCGGTGCTGACATCATTGCAGGTACTTTTTTTTGTTTTTTGTTTTTCCTTTTTTATCCTTTTCTGGTTTTGTTTACGTTATAACACGTCACCATTTTTTTTTACATCAACATCTGGATGGGAGATTCGAACCTCTAATCTTGAGCTATGCTTGCTTTGGTCACGTCACCAATCTTTTTTTCTAATAAAATAAAACTTGGTGTATAGACCAAAGTGGATAATTTATTTTAACAAAAACAATATCCAACATTTAATAAAAATCTTCATGTTGAACACTACCATTAAATTTTTTATAGCTATATATATTTGTAAAAGAAAGTAAAAAAGAAAAAAGGTATATATTCTTTTCATTTATTTTGTTTTTTTTCCTTCTTGGTAACAAAATTTAATGTTCATCAATAGGGGATTTTGTTGATTTTCTAATCTATAAGTATATAACCCTTCAAGTTTTAAAAGAAGTGGCACAAATATGAGCTCCTATCAATACAATTTTATTGGACATATATATATATATACATATATATAGAAAAGTATTAACTTTTTTTTAAAAAAATATTAACTTAACTTACCTATTCAAATGTGATAGAAATTGGCTTGTCCAAATACGTGACATTAAAAAATGAGTAAAAGTAAGATTAGTAAGAACTATTGAGTTGTCAAAGCGAGCATAGCTTAGTGTACTTCGACCAAAAGGTCAGTTTGACCAAAAGGTCAGTTTAAATTCCCCCACCTCTAATTGTTCTAAAAAAAAAAGAAAAAAAAAAGAAAAAAAAAGTAAGAACTATCGAGTTAAGAAGAAGTCAATACGAATCAAATAATTATTTAGAAAATAAAAAATTGAATAAGATTAATGATATGATAAATAAGGACTAGAAAATAAAGTATTAAAATTTTCATTTACGAAAATTAAAAAAAAATGACGTGGACTCATGAGTACCACAAAACTATCCATGAATAAATATTATATATATATATATATATAAAACAATGGAGGAATATTTTTTTTAGTGCATTGTGACATGATTAGAAGAGGTACTATTTTGTCTAGTTTTGTTTCCATGACTACTGGAAAAAGTGGGAAGTAACATCATATTTCATTGTTAATGGAGGCTTTTCTCTTAAAATATAGGTCAAAGGATAAAGTGGTGATAATGTTTTCGAGATTCTTCTTTCTTTTTTCCACGCCCCAATACTTATCGTAACTTCACTAAATATTACATCATTATATATGTAGTGATAGAATATATGTAATCGAAAAATATTTGATACTAAATTGAAATTGAAAAAAGATCCACGTATTAGGGACTATATTTTTTTTTAATTCAATGATTCTAGGACAGGAATCAAACTATAGAATTTTGAATGATAATTGATGTATGTTTAGATTGTCAAGTATTAGGGATTAAAAAGAATATATTTAATCATTTTGGGTTAAATTTTTTTATTTTTTATTTAACGTGATGAAAAACAAAATAGAAATGTCTTTTTTTTTTCAGAAAAATGTTCTTTTTATTTTTCTAATTTATTTTTTATAAAAAGAGATTGAGAAATATTGATGGTGGATGGGGTCATGTCATGTGATGACAGACTATGGGTTTTGTGGTTTTGGTTCCTCGTGAAGTATGACTCATTTGGCGTGTTCTATGCCCTTCTTTAACGTGTCACCACTTTTTCAAAAAGTGCTTTTTTTGGGAAAGGGATGGATGTTTTCTTTTTCCATAAAGTTACCAAGATTCCCATCTAAAACGTTCTTGGATGAGATGAAGTTTATCTCGGTAATGAACAGGGTAATTCATGTATCTTATAAATTGTGAGATATTCATTTTATCTTTTTCAATGTGGGATTTTCAACAAAATGGAATAGAAATGCCTTTTAATTATTGAGAATTTCGAATTGGCTAAAATAAATGTTAAAAAAGAATAAATATATATGTATGTATACACATTGTAAGTTTTTAAATTTTAACTCTAATAGTGATTTGACGTGGTATAAAAACAACTTATTCAAACTAGAGCACAACTCAGTAAACAAAATATATATACCATAAACTTACCATCTTAATTTTTTAGTTTAAAAGCACTTAATGTAGAAATAACTCGATTGGATGTGGTGTGGTAGGTGCGGTGTGTGGCCCTGAAGAGCTTGTGAACTCAATGATGGACCTACGTCAAGGGAGTCTAATGCTTCTAGGGCCAACCATGAATGCAAAGGTGGCATTTGAGCTATCAGAAAGAATCCCTCACCTAAGCATGAGAATGAAGGAGCATTGTAGAAGAGCAGCAGTGTTCGCGGAGCGAATGAAGAAGTTAGGGCTAAAGGTCATATACCCAGGACTACCAGACCACCCGCAACACCAGCTAATGAAGAGTCTTGCGAACTCGGAATATGGGTACGGAGGAATGTTGTGCCTCGACATGGAGACCGAGGAAAGGGCCAACAAGCTCATGAACGTGCTCCAAAACACCACCCAATTTGGGTTAATGGCTGTGAGTTTAGGGTACTATGAAACCCTAATGTCTTGCTCAGGCAGCAGCACAAGCAGTGAAATGAGTAGAGAGGATAGGGAATTGGCTGGAATCTCACCAGGACTCATAAGAATGTCCATCGGATATATGGGCACATTGGAGCAAAAATGGAGCCAATTTGAAAAGGCTCTTGCCAAAGTGCAAGACGTGGGACTTCCATTTTGCAAGAATTAA

mRNA sequence

ATGGCGGAATTCAAAACCGGCACAAAAAGACCGACCTCCGATGAAACCGACGAGAATTTGATCTCGAAGAAACCAACGGCCATGATTTCGTCTCTCCTCGAGGATCCAGCGGCTGCGATCGCCAACACGCGTCACGAGTTCGGCGAGCACGGAGGGGTAAATATGTCAATCGAAGCCTCCGCCACATTCACCGTCATGGAACCGGAGACCATGCGGCGGATGTTCGCCGGCGAGCTCGGCCCCGACCGTGACTTCTTCATCTACAGCCGCCATTTCAATCCGACGGTGCTGAATCTCAGCCGCCAGATGGCCGCCCTCGAAGGCACCGCCGCCGCCTACTGCACCTCCAGCGGAATGTCGGCCATCGCCGCCGTGCTCCTCCAGCTCCTCGCCAGTGGGGACCACGTCGTGGCGTCGAGAACGCTCTACGGTGGGACCCATGCGCTGCTGTCGCATTTTTTGCCGAGGACGTCGAACATAACGACGTCGTTTGTGGACGTAAGTGATTTTGAGATGGTGAGGAGGGAGATCGTTGAAGGGAAAACGAAAGTGCTTTATTTCGAAGCGGTTTCGAATCCGACTTTGGCGGTGGCGGATATACCGGAGTTGTGCCGGATTGGACATGAAAAGGGGCTGACGGTGGTCGTCGATAATACTTTTGCGCCGATGGTTCTGTCGCCGGCGAGGCTCGGTGCTGACGTGGTTGTTCATAGTATTTCTAAGTACATTAGCGGCGGTGCTGACATCATTGCAGGTGCGGTGTGTGGCCCTGAAGAGCTTGTGAACTCAATGATGGACCTACGTCAAGGGAGTCTAATGCTTCTAGGGCCAACCATGAATGCAAAGGTGGCATTTGAGCTATCAGAAAGAATCCCTCACCTAAGCATGAGAATGAAGGAGCATTGTAGAAGAGCAGCAGTGTTCGCGGAGCGAATGAAGAAGTTAGGGCTAAAGGTCATATACCCAGGACTACCAGACCACCCGCAACACCAGCTAATGAAGAGTCTTGCGAACTCGGAATATGGGTACGGAGGAATGTTGTGCCTCGACATGGAGACCGAGGAAAGGGCCAACAAGCTCATGAACGTGCTCCAAAACACCACCCAATTTGGGTTAATGGCTGTGAGTTTAGGGTACTATGAAACCCTAATGTCTTGCTCAGGCAGCAGCACAAGCAGTGAAATGAGTAGAGAGGATAGGGAATTGGCTGGAATCTCACCAGGACTCATAAGAATGTCCATCGGATATATGGGCACATTGGAGCAAAAATGGAGCCAATTTGAAAAGGCTCTTGCCAAAGTGCAAGACGTGGGACTTCCATTTTGCAAGAATTAA

Coding sequence (CDS)

ATGGCGGAATTCAAAACCGGCACAAAAAGACCGACCTCCGATGAAACCGACGAGAATTTGATCTCGAAGAAACCAACGGCCATGATTTCGTCTCTCCTCGAGGATCCAGCGGCTGCGATCGCCAACACGCGTCACGAGTTCGGCGAGCACGGAGGGGTAAATATGTCAATCGAAGCCTCCGCCACATTCACCGTCATGGAACCGGAGACCATGCGGCGGATGTTCGCCGGCGAGCTCGGCCCCGACCGTGACTTCTTCATCTACAGCCGCCATTTCAATCCGACGGTGCTGAATCTCAGCCGCCAGATGGCCGCCCTCGAAGGCACCGCCGCCGCCTACTGCACCTCCAGCGGAATGTCGGCCATCGCCGCCGTGCTCCTCCAGCTCCTCGCCAGTGGGGACCACGTCGTGGCGTCGAGAACGCTCTACGGTGGGACCCATGCGCTGCTGTCGCATTTTTTGCCGAGGACGTCGAACATAACGACGTCGTTTGTGGACGTAAGTGATTTTGAGATGGTGAGGAGGGAGATCGTTGAAGGGAAAACGAAAGTGCTTTATTTCGAAGCGGTTTCGAATCCGACTTTGGCGGTGGCGGATATACCGGAGTTGTGCCGGATTGGACATGAAAAGGGGCTGACGGTGGTCGTCGATAATACTTTTGCGCCGATGGTTCTGTCGCCGGCGAGGCTCGGTGCTGACGTGGTTGTTCATAGTATTTCTAAGTACATTAGCGGCGGTGCTGACATCATTGCAGGTGCGGTGTGTGGCCCTGAAGAGCTTGTGAACTCAATGATGGACCTACGTCAAGGGAGTCTAATGCTTCTAGGGCCAACCATGAATGCAAAGGTGGCATTTGAGCTATCAGAAAGAATCCCTCACCTAAGCATGAGAATGAAGGAGCATTGTAGAAGAGCAGCAGTGTTCGCGGAGCGAATGAAGAAGTTAGGGCTAAAGGTCATATACCCAGGACTACCAGACCACCCGCAACACCAGCTAATGAAGAGTCTTGCGAACTCGGAATATGGGTACGGAGGAATGTTGTGCCTCGACATGGAGACCGAGGAAAGGGCCAACAAGCTCATGAACGTGCTCCAAAACACCACCCAATTTGGGTTAATGGCTGTGAGTTTAGGGTACTATGAAACCCTAATGTCTTGCTCAGGCAGCAGCACAAGCAGTGAAATGAGTAGAGAGGATAGGGAATTGGCTGGAATCTCACCAGGACTCATAAGAATGTCCATCGGATATATGGGCACATTGGAGCAAAAATGGAGCCAATTTGAAAAGGCTCTTGCCAAAGTGCAAGACGTGGGACTTCCATTTTGCAAGAATTAA

Protein sequence

MAEFKTGTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRREIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSMRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEERANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGYMGTLEQKWSQFEKALAKVQDVGLPFCKN

Homology

[Close]

BLAST of Lag0017210 vs. NCBI nr
Match: XP_023538834.1 (methionine gamma-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798.5 bits (2061), Expect = 2.9e-227
Identity = 401/448 (89.51%), Postives = 426/448 (95.09%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE K     G KRP + ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQKSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 180
           SGMSAIAAV LQLLASGDHVVA+RTLYGGTHALL+HFLPRTSNITTSFVD+SD EMV + 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240
           IVEGKTKVLYFEA++NPTL VA+IPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSM 300
           HSISK+ISGGADIIAGAVCGP +LVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLS+
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKS+ NSEYG+GG+LCLDM TEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGY 420
           ANKLMNVLQNTTQFG +AVSLGYYETLMSCSGSSTSSE+S +D+ELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           MGTLEQKWSQFEKALAKVQD+GLP+C N
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLGLPYCNN 448

[Close]

BLAST of Lag0017210 vs. NCBI nr
Match: KAG7028211.1 (Methionine gamma-lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 797.0 bits (2057), Expect = 8.5e-227
Identity = 400/448 (89.29%), Postives = 425/448 (94.87%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE K     G KRP + ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 180
           SGMSAIAAV LQLLASGDHVVA+RTLYGGTHALL+HFLPRTSNITTSFVD+SD EMV + 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240
           IVEGKTKVLYFEA++NPTL VA+IPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSM 300
           HSISK+ISGGADIIAGAVCGP +LVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLS+
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKS+ NSEYG+GG+LCLDM TEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGY 420
           ANKLMNVLQNTTQFG +AVSLGYYETLMSCSGSSTSSE+S +D+ELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           MGTLEQKWSQFEKAL KVQD+GLP+C N
Sbjct: 421 MGTLEQKWSQFEKALTKVQDLGLPYCNN 448

[Close]

BLAST of Lag0017210 vs. NCBI nr
Match: KAG6596676.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 796.2 bits (2055), Expect = 1.4e-226
Identity = 400/448 (89.29%), Postives = 425/448 (94.87%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE K       KRP + ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCSAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 180
           SGMSAIAAV LQLLASGDHVVA+RTLYGGTHALL+HFLPRTSNITTSFVD+SD EMV + 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240
           IVEGKTKVLYFEA++NPTL VA+IPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSM 300
           HSISK+ISGGADIIAGAVCGP +LVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLS+
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKS+ NSEYG+GG+LCLDM TEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGY 420
           ANKLMNVLQNTTQFG +AVSLGYYETLMSCSGSSTSSE+S +D+ELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           MGTLEQKWSQFEKALAKVQD+GLP+C N
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLGLPYCNN 448

[Close]

BLAST of Lag0017210 vs. NCBI nr
Match: XP_022950227.1 (methionine gamma-lyase-like [Cucurbita moschata])

HSP 1 Score: 793.9 bits (2049), Expect = 7.2e-226
Identity = 398/448 (88.84%), Postives = 425/448 (94.87%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE K     G KRP + ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 180
           SGMSAIAAV LQLLASGDHVVA+RTLYGGTHALL+HFLPRTSNITTSFVD+SD EMV + 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240
           IVEGKTKVLYFEA++NPTL VA+IPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSM 300
           HSISK+ISGGADIIAGAVCGP +LVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLS+
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQL+KS+ NSEYG+GG+LCLDM TEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLLKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGY 420
           ANKLMNVLQNTTQFG +AVSLGYYETLMSCSGSSTSSE+S +D+ELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           MGTLEQKWSQFEKAL KVQ++GLP+C N
Sbjct: 421 MGTLEQKWSQFEKALTKVQNLGLPYCNN 448

[Close]

BLAST of Lag0017210 vs. NCBI nr
Match: XP_023005861.1 (methionine gamma-lyase-like [Cucurbita maxima])

HSP 1 Score: 791.6 bits (2043), Expect = 3.6e-225
Identity = 400/448 (89.29%), Postives = 422/448 (94.20%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE K     G KRP + ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNFCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGP+RDFFIYSRHFNPTVLNLSR +AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPERDFFIYSRHFNPTVLNLSRLIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 180
           SGMSAIAAV LQLLASGDHVVA+RTLYGGTHALL+HFLPRTSNITTSFVD+SD EMV + 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240
           IVEGKTKVLYFEA++NPTL VA+IPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSM 300
           HSISK+ISGGADIIAGAVCGP +LVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLS+
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSL NSEYG GG+LCLDM TEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLGNSEYGCGGILCLDMGTEER 360

Query: 361 ANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGY 420
           ANKLMNVLQNTTQFG +AVSLGYYETLMSCS SSTSSE+S  D+ELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSSSSTSSELSGNDKELAGISPGLVRMSIGY 420

Query: 421 MGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           MGTLEQKWSQFEKALAKVQD+GLPFC N
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLGLPFCNN 448

[Close]

BLAST of Lag0017210 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.2e-185
Identity = 326/430 (75.81%), Postives = 369/430 (85.81%), Query Frame = 0


Query: 6   TGTKRPTSDETD-ENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFT 65
           +G KR   D+ D + L++KK    +     DPAAAIAN RHEFGEHGGVNMSIEASATFT
Sbjct: 14  SGKKRNDRDDEDGDALVAKKSALAVCD--ADPAAAIANIRHEFGEHGGVNMSIEASATFT 73

Query: 66  VMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAA 125
           VMEP+TMRRMF GELGPD DF++YSRHFNPTVLNLSRQMAALEGT AAYCTSSGMSAI++
Sbjct: 74  VMEPDTMRRMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISS 133

Query: 126 VLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRREIVEGKTKV 185
           V+LQL +SG HVVA+ TLYGGTHALLSHFLPRT NITTSFVD++D   V   IVEG+T+V
Sbjct: 134 VMLQLCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQV 193

Query: 186 LYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKYIS 245
           LYFE+V+NPTL VADIPEL R+ HEKG+TVVVDNTFAPMVLSPA+LGADVVVHSISK+IS
Sbjct: 194 LYFESVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFIS 253

Query: 246 GGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSMRMKEHCRR 305
           GGADIIAGAVCG E LV  MMDLR GSLMLLGPTMNAKVAFELSERIPHL +RM+EH  R
Sbjct: 254 GGADIIAGAVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHR 313

Query: 306 AAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEERANKLMNVL 365
           A V+AERM+ LG+KVIYPGL  HPQH+L K + N +YGYGG+L +DMETEE+ANKLM  L
Sbjct: 314 AQVYAERMRDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLLSIDMETEEKANKLMAYL 373

Query: 366 QNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGYMGTLEQKW 425
           QN TQFG MAVSLGYYETLMSCSGSSTSSE+    +E AGISPGL+RMS+GY+GTLEQKW
Sbjct: 374 QNATQFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKW 433

Query: 426 SQFEKALAKV 435
           +QFEKA  ++
Sbjct: 434 TQFEKAFLRM 441

[Close]

BLAST of Lag0017210 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.5e-53
Identity = 143/389 (36.76%), Postives = 215/389 (55.27%), Query Frame = 0


Query: 50  HGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGT 109
           +G + M I  ++TF     E   R FA E       +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALE----EAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 110 AAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSD 169
            AA  TSSGM AI++ L  +L +GDHVV  +TLYG T AL+ H L R   I  +FVD S+
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTR-FGIEVTFVDTSN 141

Query: 170 FEMVRREIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLT-VVVDNTFA-PMVLSP 229
            + V +  ++  T+V+Y E  +NP L + D+  L ++ H    T V+VDNTFA P +  P
Sbjct: 142 LDEV-KNAMKKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 230 ARLGADVVVHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFEL 289
            +LGAD+VVHS++KYI+G  D+IAG V   +EL + +  +  G   + G  +  + A+ +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 290 SERIPHLSMRMKEHCRRAAVFAERMKKLGL--KVIYPGLPDHPQHQLMKSLANSEYGYGG 349
              +    +RM+ HC+ A    E + K     +V YPGL  HP H++ K        +G 
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIERVYYPGLETHPGHEIAKKQMKD---FGA 321

Query: 350 MLCLDMETEERANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGI 409
           M+  +++    A K    L N  +   +AVSLG  ETL+    S T S  ++E+RE AGI
Sbjct: 322 MISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREAAGI 381

Query: 410 SPGLIRMSIGYMGTLEQKWSQFEKALAKV 435
           + GL+R+S+G +  +E   +  E+ L K+
Sbjct: 382 TDGLVRLSVG-LENVEDIIADLEQGLEKI 395

[Close]

BLAST of Lag0017210 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 4.9e-52
Identity = 139/389 (35.73%), Postives = 213/389 (54.76%), Query Frame = 0


Query: 50  HGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGT 109
           +G + M I  ++TF     E   R FA E       +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALE----EAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 110 AAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSD 169
            A    SSGM AI++ L  +L +GDHVV  +TLYG T AL++H L R   +  +FVD S+
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTR-FGVEVTFVDTSN 141

Query: 170 FEMVRREIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLT-VVVDNTFA-PMVLSP 229
            E V +  ++  T+V+Y E  +NP L + D+  L +I H    T V+VDNTFA P +  P
Sbjct: 142 LEEV-KNAMKKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 230 ARLGADVVVHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFEL 289
            +LG D+VVHS +KY++G  D+IAG V   +EL + +  +  G   + G  +  + A+ +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 290 SERIPHLSMRMKEHCRRAAVFAERMKKLGL--KVIYPGLPDHPQHQLMKSLANSEYGYGG 349
              +    +RM+ HC+ A    + + K     KV YPGL  HP +++ K        +G 
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGYEIAKKQMKD---FGA 321

Query: 350 MLCLDMETEERANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGI 409
           M+  +++    A K    L N  +   +AVSLG  ETL+    S T S  ++E+RE+AGI
Sbjct: 322 MISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREVAGI 381

Query: 410 SPGLIRMSIGYMGTLEQKWSQFEKALAKV 435
           + GL+R+S+G +  +E   +  E+ L K+
Sbjct: 382 TDGLVRLSVG-LENVEDIIADLEQGLEKI 395

[Close]

BLAST of Lag0017210 vs. ExPASy Swiss-Prot
Match: Q7MX71 (L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mgl PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 3.9e-49
Identity = 136/364 (37.36%), Postives = 200/364 (54.95%), Query Frame = 0


Query: 57  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 116
           I  ++TF     E   R FAGE     D +IY+R  NP    +  ++A LEG  AA   S
Sbjct: 32  IYQTSTFVFDTAEQGGRRFAGE----EDGYIYTRLGNPNCTQVEEKLAMLEGGEAAASAS 91

Query: 117 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 176
           SG+ AI++ +   + +GDH+VA +TLYG T A L+H L R   +  + VD    E V   
Sbjct: 92  SGIGAISSAIWVCVKAGDHIVAGKTLYGCTFAFLTHGLSR-YGVEVTLVDTRHPEEVEAA 151

Query: 177 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHE-KGLTVVVDNTF-APMVLSPARLGADV 236
           I    TK++Y E  +NP + + DI  +C I H+ +G+ V+VDNT+  P +  P  LGAD+
Sbjct: 152 I-RPNTKLVYLETPANPNMYLTDIKAVCDIAHKHEGVRVMVDNTYCTPYICRPLELGADI 211

Query: 237 VVHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHL 296
           VVHS +KY++G  D+IAG V G E+ +  +  +  G   L G  M+   A+ +S  +  L
Sbjct: 212 VVHSATKYLNGHGDVIAGFVVGKEDYIKEVKLV--GVKDLTGANMSPFDAYLISRGMKTL 271

Query: 297 SMRMKEHCRRAAVFAERMKKLGL--KVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDME 356
            +RM++HCR A   AE ++K      V +PGLP  PQ++L K         G M+  +++
Sbjct: 272 QIRMEQHCRNAQTVAEFLEKHPAVEAVYFPGLPSFPQYELAKKQMALP---GAMIAFEVK 331

Query: 357 TE-ERANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIR 416
              E   KLMN L   +    +AVSLG  ETL+    S T S  + E+R  + IS GL+R
Sbjct: 332 GGCEAGKKLMNNLHLCS----LAVSLGDTETLIQHPASMTHSPYTPEERAASDISEGLVR 380

[Close]

BLAST of Lag0017210 vs. ExPASy Swiss-Prot
Match: P13254 (L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 8.1e-47
Identity = 140/373 (37.53%), Postives = 199/373 (53.35%), Query Frame = 0


Query: 49  EHGGVNM-SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALE 108
           +HGG  +  +  +ATFT    E     FAGE    +    YSR  NPT+  L  +MA+LE
Sbjct: 23  DHGGALVPPVYQTATFTFPTVEYGAACFAGE----QAGHFYSRISNPTLNLLEARMASLE 82

Query: 109 GTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDV 168
           G  A    +SGM AI + L  LL  GD V+   TLYG T A L H +     +    VD+
Sbjct: 83  GGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGE-FGVKLRHVDM 142

Query: 169 SDFEMVRREIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTF-APMVLS 228
           +D + +   +    T+V+YFE+ +NP + +ADI  + +I  + G TVVVDNT+  P +  
Sbjct: 143 ADLQALEAAMTPA-TRVIYFESPANPNMHMADIAGVAKIARKHGATVVVDNTYCTPYLQR 202

Query: 229 PARLGADVVVHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFE 288
           P  LGAD+VVHS +KY+SG  DI AG V G + LV+ +    QG   + G  ++   A  
Sbjct: 203 PLELGADLVVHSATKYLSGHGDITAGIVVGSQALVDRIR--LQGLKDMTGAVLSPHDAAL 262

Query: 289 LSERIPHLSMRMKEHCRRAAVFAE---RMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGY 348
           L   I  L++RM  HC  A V AE   R  ++ L + YPGL   PQ+ L +   +     
Sbjct: 263 LMRGIKTLNLRMDRHCANAQVLAEFLARQPQVEL-IHYPGLASFPQYTLARQQMSQP--- 322

Query: 349 GGMLCLDMETEERA-NKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDREL 408
           GGM+  +++    A  + MN LQ  ++    AVSLG  E+L     S T S  + E+R  
Sbjct: 323 GGMIAFELKGGIGAGRRFMNALQLFSR----AVSLGDAESLAQHPASMTHSSYTPEERAH 379

Query: 409 AGISPGLIRMSIG 416
            GIS GL+R+S+G
Sbjct: 383 YGISEGLVRLSVG 379

[Close]

BLAST of Lag0017210 vs. ExPASy TrEMBL
Match: A0A6J1GE83 (methionine gamma-lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111453379 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.5e-226
Identity = 398/448 (88.84%), Postives = 425/448 (94.87%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE K     G KRP + ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 180
           SGMSAIAAV LQLLASGDHVVA+RTLYGGTHALL+HFLPRTSNITTSFVD+SD EMV + 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240
           IVEGKTKVLYFEA++NPTL VA+IPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSM 300
           HSISK+ISGGADIIAGAVCGP +LVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLS+
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQL+KS+ NSEYG+GG+LCLDM TEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLLKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGY 420
           ANKLMNVLQNTTQFG +AVSLGYYETLMSCSGSSTSSE+S +D+ELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           MGTLEQKWSQFEKAL KVQ++GLP+C N
Sbjct: 421 MGTLEQKWSQFEKALTKVQNLGLPYCNN 448

[Close]

BLAST of Lag0017210 vs. ExPASy TrEMBL
Match: A0A6J1KW67 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111498738 PE=3 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.7e-225
Identity = 400/448 (89.29%), Postives = 422/448 (94.20%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE K     G KRP + ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNFCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGP+RDFFIYSRHFNPTVLNLSR +AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPERDFFIYSRHFNPTVLNLSRLIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRRE 180
           SGMSAIAAV LQLLASGDHVVA+RTLYGGTHALL+HFLPRTSNITTSFVD+SD EMV + 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240
           IVEGKTKVLYFEA++NPTL VA+IPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSM 300
           HSISK+ISGGADIIAGAVCGP +LVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLS+
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSL NSEYG GG+LCLDM TEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLGNSEYGCGGILCLDMGTEER 360

Query: 361 ANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGY 420
           ANKLMNVLQNTTQFG +AVSLGYYETLMSCS SSTSSE+S  D+ELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSSSSTSSELSGNDKELAGISPGLVRMSIGY 420

Query: 421 MGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           MGTLEQKWSQFEKALAKVQD+GLPFC N
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLGLPFCNN 448

[Close]

BLAST of Lag0017210 vs. ExPASy TrEMBL
Match: M1NFB7 (Methionine gamma-lyase OS=Cucumis melo OX=3656 GN=MGL PE=2 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 6.6e-225
Identity = 399/449 (88.86%), Postives = 424/449 (94.43%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDE-NLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60
           MAE K     GTKRP+ DE+D+    SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM
Sbjct: 1   MAELKNHNFRGTKRPSPDESDDVKTDSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60

Query: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120
           SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT
Sbjct: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120

Query: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRR 180
           SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHAL++HF PRTSNITT+FVD+ D + V +
Sbjct: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDIGDLKEVEK 180

Query: 181 EIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVV 240
            IVEGKTKVLYFE+VSNPTLAVA+IPELCRIGHEKG+TVVVDNTFAPM+LSPARLGADVV
Sbjct: 181 AIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSPARLGADVV 240

Query: 241 VHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300
           VHSISK+ISGGADIIAGAVCGP +LVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS
Sbjct: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300

Query: 301 MRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEE 360
           +RMKEHCRRA+VFAERMKK GLKVIYPGL DHPQHQLMKSLAN EYG+GGMLC+DM TEE
Sbjct: 301 LRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGMLCVDMGTEE 360

Query: 361 RANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIG 420
           RANKLM++LQNTTQFG MAVSLGYYETLMSCSGSSTSSEMS E+RELAGISPGL+RMSIG
Sbjct: 361 RANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISPGLVRMSIG 420

Query: 421 YMGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           YMGTLEQKWSQFEKALAKVQD+G+PFC N
Sbjct: 421 YMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of Lag0017210 vs. ExPASy TrEMBL
Match: A0A5A7UHF4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00790 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.6e-224
Identity = 398/449 (88.64%), Postives = 423/449 (94.21%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDE-NLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60
           MAE K     GTKR + DE+D+    SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM
Sbjct: 1   MAELKNHNFRGTKRASPDESDDVKTDSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60

Query: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120
           SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT
Sbjct: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120

Query: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRR 180
           SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHAL++HF PRTSNITT+FVD+ D + V +
Sbjct: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDIGDLKEVEK 180

Query: 181 EIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVV 240
            IVEGKTKVLYFE+VSNPTLAVA+IPELCRIGHEKG+TVVVDNTFAPM+LSPARLGADVV
Sbjct: 181 AIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSPARLGADVV 240

Query: 241 VHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300
           VHSISK+ISGGADIIAGAVCGP +LVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS
Sbjct: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300

Query: 301 MRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEE 360
           +RMKEHCRRA+VFAERMKK GLKVIYPGL DHPQHQLMKSLAN EYG+GGMLC+DM TEE
Sbjct: 301 LRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGMLCVDMGTEE 360

Query: 361 RANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIG 420
           RANKLM++LQNTTQFG MAVSLGYYETLMSCSGSSTSSEMS E+RELAGISPGL+RMSIG
Sbjct: 361 RANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISPGLVRMSIG 420

Query: 421 YMGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           YMGTLEQKWSQFEKALAKVQD+G+PFC N
Sbjct: 421 YMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of Lag0017210 vs. ExPASy TrEMBL
Match: A0A1S3BKU9 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103490704 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.6e-224
Identity = 398/449 (88.64%), Postives = 423/449 (94.21%), Query Frame = 0


Query: 1   MAEFKT----GTKRPTSDETDE-NLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60
           MAE K     GTKR + DE+D+    SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM
Sbjct: 1   MAELKNHNFRGTKRASPDESDDVKTDSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60

Query: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120
           SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT
Sbjct: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120

Query: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRR 180
           SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHAL++HF PRTSNITT+FVD+ D + V +
Sbjct: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDIGDLKEVEK 180

Query: 181 EIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVV 240
            IVEGKTKVLYFE+VSNPTLAVA+IPELCRIGHEKG+TVVVDNTFAPM+LSPARLGADVV
Sbjct: 181 AIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSPARLGADVV 240

Query: 241 VHSISKYISGGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300
           VHSISK+ISGGADIIAGAVCGP +LVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS
Sbjct: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300

Query: 301 MRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEE 360
           +RMKEHCRRA+VFAERMKK GLKVIYPGL DHPQHQLMKSLAN EYG+GGMLC+DM TEE
Sbjct: 301 LRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGMLCVDMGTEE 360

Query: 361 RANKLMNVLQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIG 420
           RANKLM++LQNTTQFG MAVSLGYYETLMSCSGSSTSSEMS E+RELAGISPGL+RMSIG
Sbjct: 361 RANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISPGLVRMSIG 420

Query: 421 YMGTLEQKWSQFEKALAKVQDVGLPFCKN 445
           YMGTLEQKWSQFEKALAKVQD+G+PFC N
Sbjct: 421 YMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of Lag0017210 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 649.8 bits (1675), Expect = 1.6e-186
Identity = 326/430 (75.81%), Postives = 369/430 (85.81%), Query Frame = 0


Query: 6   TGTKRPTSDETD-ENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFT 65
           +G KR   D+ D + L++KK    +     DPAAAIAN RHEFGEHGGVNMSIEASATFT
Sbjct: 14  SGKKRNDRDDEDGDALVAKKSALAVCD--ADPAAAIANIRHEFGEHGGVNMSIEASATFT 73

Query: 66  VMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAA 125
           VMEP+TMRRMF GELGPD DF++YSRHFNPTVLNLSRQMAALEGT AAYCTSSGMSAI++
Sbjct: 74  VMEPDTMRRMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISS 133

Query: 126 VLLQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRREIVEGKTKV 185
           V+LQL +SG HVVA+ TLYGGTHALLSHFLPRT NITTSFVD++D   V   IVEG+T+V
Sbjct: 134 VMLQLCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQV 193

Query: 186 LYFEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKYIS 245
           LYFE+V+NPTL VADIPEL R+ HEKG+TVVVDNTFAPMVLSPA+LGADVVVHSISK+IS
Sbjct: 194 LYFESVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFIS 253

Query: 246 GGADIIAGAVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSMRMKEHCRR 305
           GGADIIAGAVCG E LV  MMDLR GSLMLLGPTMNAKVAFELSERIPHL +RM+EH  R
Sbjct: 254 GGADIIAGAVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHR 313

Query: 306 AAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEERANKLMNVL 365
           A V+AERM+ LG+KVIYPGL  HPQH+L K + N +YGYGG+L +DMETEE+ANKLM  L
Sbjct: 314 AQVYAERMRDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLLSIDMETEEKANKLMAYL 373

Query: 366 QNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIGYMGTLEQKW 425
           QN TQFG MAVSLGYYETLMSCSGSSTSSE+    +E AGISPGL+RMS+GY+GTLEQKW
Sbjct: 374 QNATQFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKW 433

Query: 426 SQFEKALAKV 435
           +QFEKA  ++
Sbjct: 434 TQFEKAFLRM 441

[Close]

BLAST of Lag0017210 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.2e-39
Identity = 107/335 (31.94%), Postives = 178/335 (53.13%), Query Frame = 0


Query: 86  FIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGG 145
           F Y R+ NPT + L  +++ALEG  +    +SGM A   +LL L+ +G H+V +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 146 THALLSHFLPRTSNITTSFVDVSDFEMVRREIVEGKTKVLYFEAVSNPTLAVADIPELCR 205
           T   + +FLP+   IT + +D +D   +   + E K  + + E+ +NP L   DI  + +
Sbjct: 284 TRIFMENFLPKL-GITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 206 IGHEKGLTVVVDNTFA-PMVLSPARLGADVVVHSISKYISGGADIIAGAVCGPEELVNSM 265
           I H++G  V +D TFA P+      LGAD+VVHS +KYI G  D++AG +CG  +LV+ +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 266 MDLRQGSLMLLGPTMNAKVAFELSERIPHLSMRMKEH---CRRAAVFAERMKKLGLKVIY 325
            +L      +LG T+N   A+ +   +  L +R+++      R A   E   K+   V Y
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVS-HVYY 463

Query: 326 PGLPDHPQHQLMKSLANSEYGYGGMLCLDMETE-ERANKLMNVLQNTTQFGLMAVSLGYY 385
           PGLP HP+H+L K       G+GG++  +++ + E   K ++ L    +   +A S G  
Sbjct: 464 PGLPSHPEHELAK---RQMTGFGGVVSFEIDGDIETTIKFVDSL----KIPYIAPSFGGC 523

Query: 386 ETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIG 416
           E+++      +  ++ +E+R   GI   L+R S G
Sbjct: 524 ESIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFG 545

[Close]

BLAST of Lag0017210 vs. TAIR 10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 3.1e-33
Identity = 100/337 (29.67%), Postives = 173/337 (51.34%), Query Frame = 0


Query: 84  DFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLY 143
           D + Y+R+ NPT + L  +++ LEG  +    +SGM A   +LL L+ +  H+VA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 144 GGTHALLSHFLPRTSNITTSFVDVSDFEMVRREIVEGKTKVLYFEAVSNPTLAVADIPEL 203
             T   + +FL +   IT +F+D  D   ++  +   +  + + E+ +NP L   DI  +
Sbjct: 132 KETRIFMENFLTKL-GITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLV 191

Query: 204 CRIGHEKGLTVVVDNTFA-PMVLSPARLGADVVVHSISKYISGGADIIAGAVCGPEELVN 263
            +I H +G  V +D T A P+      LGAD+V HS +KYI G  D +AG++ G  ELV+
Sbjct: 192 SKICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVS 251

Query: 264 SMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSMRMKEH---CRRAAVFAERMKKLGLKV 323
            + +L +    LLG T+N   A+ L   +  + +R+++      + A   E   K+  +V
Sbjct: 252 KIRNLHK----LLGGTLNPNAAYLLIRGMKTMHLRVRQQNSTGMKMAQVLEAHPKVS-RV 311

Query: 324 IYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEERAN-KLMNVLQNTTQFGLMAVSLG 383
            Y GLP HP+H + K       G GG++  +++ + +   K ++ L    +   +A S G
Sbjct: 312 YYLGLPSHPEHLIAK---RQMTGIGGLISFEIDGDLKTTIKFIDAL----KIPYLAASFG 371

Query: 384 YYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIG 416
             E+L+    +    ++ RE+R   G    L+R S G
Sbjct: 372 GCESLVDQLATGI-WDIPREERLKDGFQDNLVRFSFG 394

[Close]

BLAST of Lag0017210 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 139.8 bits (351), Expect = 5.3e-33
Identity = 123/412 (29.85%), Postives = 211/412 (51.21%), Query Frame = 0


Query: 8   TKRPTSDETDE-NLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVM 67
           T    ++ TD  N ++ K  A +S+LL        N  ++F     ++  +  +ATF   
Sbjct: 65  THSTVNNTTDSLNTMNIKEEASVSTLL-------VNLDNKFDPFDAMSTPLYQTATF--- 124

Query: 68  EPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAVL 127
                ++  A E GP    + Y+R  NPT   L   +A L+    A+C +SGM+A++AV 
Sbjct: 125 -----KQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAALSAV- 184

Query: 128 LQLLASGDHVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRREIVEGKTKVLY 187
             L+ +G+ +VA   +YGG+  LLS  +PR S +    V+ +  + V   I   +TK+++
Sbjct: 185 THLIKNGEEIVAGDDVYGGSDRLLSQVVPR-SGVVVKRVNTTKLDEVAAAI-GPQTKLVW 244

Query: 188 FEAVSNPTLAVADIPELCRIGHEKGLTVVVDNTFAPMVLS-PARLGADVVVHSISKYISG 247
            E+ +NP   ++DI ++  + H +G  V+VDN+    VLS P  LGAD+V+HS +K+I+G
Sbjct: 245 LESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 304

Query: 248 GADIIAG--AVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSMRMKEHCR 307
            +D++AG  AV G E+L   +  L+      L P  +  +     + +     + +E+ R
Sbjct: 305 HSDVMAGVLAVKG-EKLAKEVYFLQNSEGSGLAP-FDCWLCLRGIKTMALRIEKQQENAR 364

Query: 308 RAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEERANKLMNV 367
           + A++     ++  KV Y GLPDHP H L  S A    G G +      +   +  L+  
Sbjct: 365 KIAMYLSSHPRV-KKVYYAGLPDHPGHHLHFSQAK---GAGSVFSFITGSVALSKHLV-- 424

Query: 368 LQNTTQFGLMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIG 416
              TT++  +AVS G  ++L+S     + + +  E RE  G++  L+R+S G
Sbjct: 425 --ETTKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAG 444

[Close]

BLAST of Lag0017210 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-32
Identity = 120/404 (29.70%), Postives = 207/404 (51.24%), Query Frame = 0


Query: 15  ETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETMRRM 74
           E   + ++ K  A +S+LL        N  ++F     ++  +  +ATF        ++ 
Sbjct: 58  EKSVDAMNIKEEASVSTLL-------VNLDNKFDPFDAMSTPLYQTATF--------KQP 117

Query: 75  FAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAVLLQLLASGD 134
            A E GP    + Y+R  NPT   L   +A L+    A+C +SGM+A++AV   L+ +G+
Sbjct: 118 SAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAALSAV-THLIKNGE 177

Query: 135 HVVASRTLYGGTHALLSHFLPRTSNITTSFVDVSDFEMVRREIVEGKTKVLYFEAVSNPT 194
            +VA   +YGG+  LLS  +PR S +    V+ +  + V   I   +TK+++ E+ +NP 
Sbjct: 178 EIVAGDDVYGGSDRLLSQVVPR-SGVVVKRVNTTKLDEVAAAI-GPQTKLVWLESPTNPR 237

Query: 195 LAVADIPELCRIGHEKGLTVVVDNTFAPMVLS-PARLGADVVVHSISKYISGGADIIAG- 254
             ++DI ++  + H +G  V+VDN+    VLS P  LGAD+V+HS +K+I+G +D++AG 
Sbjct: 238 QQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAGV 297

Query: 255 -AVCGPEELVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSMRMKEHCRRAAVFAER 314
            AV G E+L   +  L+      L P  +  +     + +     + +E+ R+ A++   
Sbjct: 298 LAVKG-EKLAKEVYFLQNSEGSGLAP-FDCWLCLRGIKTMALRIEKQQENARKIAMYLSS 357

Query: 315 MKKLGLKVIYPGLPDHPQHQLMKSLANSEYGYGGMLCLDMETEERANKLMNVLQNTTQFG 374
             ++  KV Y GLPDHP H L  S A    G G +      +   +  L+     TT++ 
Sbjct: 358 HPRV-KKVYYAGLPDHPGHHLHFSQAK---GAGSVFSFITGSVALSKHLV----ETTKYF 417

Query: 375 LMAVSLGYYETLMSCSGSSTSSEMSREDRELAGISPGLIRMSIG 416
            +AVS G  ++L+S     + + +  E RE  G++  L+R+S G
Sbjct: 418 SIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAG 429

The following BLAST results are available for this feature:

BLAST of Lag0017210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023538834.1	2.9e-227	89.51	methionine gamma-lyase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7028211.1	8.5e-227	89.29	Methionine gamma-lyase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6596676.1	1.4e-226	89.29	Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022950227.1	7.2e-226	88.84	methionine gamma-lyase-like [Cucurbita moschata]	[more]
XP_023005861.1	3.6e-225	89.29	methionine gamma-lyase-like [Cucurbita maxima]	[more]

BLAST of Lag0017210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGU9	2.2e-185	75.81	Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1	[more]
Q8L0X4	1.5e-53	36.76	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ...	[more]
Q8RDT4	4.9e-52	35.73	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC...	[more]
Q7MX71	3.9e-49	37.36	L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83)...	[more]
P13254	8.1e-47	37.53	L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2	[more]

BLAST of Lag0017210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GE83	3.5e-226	88.84	methionine gamma-lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111453379 PE=3 S...	[more]
A0A6J1KW67	1.7e-225	89.29	methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111498738 PE=3 SV=...	[more]
M1NFB7	6.6e-225	88.86	Methionine gamma-lyase OS=Cucumis melo OX=3656 GN=MGL PE=2 SV=1	[more]
A0A5A7UHF4	5.6e-224	88.64	Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BKU9	5.6e-224	88.64	methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103490704 PE=3 SV=1	[more]

BLAST of Lag0017210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64660.1	1.6e-186	75.81	methionine gamma-lyase	[more]
AT3G01120.1	2.2e-39	31.94	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT1G33320.1	3.1e-33	29.67	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT3G57050.1	5.3e-33	29.85	cystathionine beta-lyase	[more]
AT3G57050.2	1.2e-32	29.70	cystathionine beta-lyase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PFAM	PF01053	Cys_Met_Meta_PP	coord: 44..415 e-value: 2.2E-85 score: 286.5
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PIRSF	PIRSF001434	CGS	coord: 23..435 e-value: 1.9E-106 score: 354.1
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PANTHER	PTHR11808	TRANS-SULFURATION ENZYME FAMILY MEMBER	coord: 26..435
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	CDD	cd00614	CGS_like	coord: 51..432 e-value: 5.79606E-133 score: 385.787
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 296..439 e-value: 3.6E-27 score: 97.1
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 37..291 e-value: 2.7E-68 score: 232.0
None	No IPR available	PIRSR	PIRSR001434-2	PIRSR001434-2	coord: 89..395 e-value: 1.0E-82 score: 275.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11808:SF76	METHIONINE GAMMA-LYASE-LIKE	coord: 26..435
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 47..430

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017210.1	Lag0017210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019343	cysteine biosynthetic process via cystathionine
biological_process	GO:0019346	transsulfuration
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004123	cystathionine gamma-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity