Lag0017154 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017154
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Protease inhibitor protein
Location	chr5: 613951 .. 614266 (+)
RNA-Seq Expression	Lag0017154
Synteny	Lag0017154

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGAATGTCCTCCAGGTATGTTCATTATTTCATTGCTTAATCACTTGTGAATATGTTTAATTTGATTGTAACTTATGTATATTAGCAAATTTAATATGAATTACGTGTTTCTAAATAGGAAAAAGCCAGTGGCCAGAGCTTGTTTTTGTAGAATATGATGTTGCTGCTGCGATCATTGAAAAGGAGAATCCCAATGTCAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTCGATTGCACACGAGTCTGGGTTGATTGCAATATTAATGGTTGGGTTGTTGAAACTCCTAAAGTTGGTTAA

mRNA sequence

ATGTCTGAATGTCCTCCAGGAAAAAGCCAGTGGCCAGAGCTTGTTTTTGTAGAATATGATGTTGCTGCTGCGATCATTGAAAAGGAGAATCCCAATGTCAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTCGATTGCACACGAGTCTGGGTTGATTGCAATATTAATGGTTGGGTTGTTGAAACTCCTAAAGTTGGTTAA

Coding sequence (CDS)

ATGTCTGAATGTCCTCCAGGAAAAAGCCAGTGGCCAGAGCTTGTTTTTGTAGAATATGATGTTGCTGCTGCGATCATTGAAAAGGAGAATCCCAATGTCAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTCGATTGCACACGAGTCTGGGTTGATTGCAATATTAATGGTTGGGTTGTTGAAACTCCTAAAGTTGGTTAA

Protein sequence

MSECPPGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGWVVETPKVG

Homology

[Close]

BLAST of Lag0017154 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 102.1 bits (253), Expect = 2.1e-18
Identity = 46/68 (67.65%), Postives = 56/68 (82.35%), Query Frame = 0


Query: 3  ECPPGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGW 62
          E  P K++WPELVFV+Y +AAAIIEKENP+V+A KIL+G+PRILNFD  RVWVD N+   
Sbjct: 4  ESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEV 63

Query: 63 VVETPKVG 71
          VV+ PKVG
Sbjct: 64 VVKKPKVG 71

[Close]

BLAST of Lag0017154 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-16
Identity = 44/63 (69.84%), Postives = 51/63 (80.95%), Query Frame = 0


Query: 8  KSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGWVVETP 67
          K++WPELVFVE+  AA IIEKENP+VKA KILA SPRI NFD +RVWVDCN+   VV+ P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 68 KVG 71
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of Lag0017154 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-16
Identity = 41/64 (64.06%), Postives = 53/64 (82.81%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGWVVE 65
          P K+QWPELVF++Y +AAAIIE+ENP+V+A KIL+G+PRILNFD  RVW D N+   +V+
Sbjct: 8  PRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVK 67

Query: 66 TPKV 70
           PKV
Sbjct: 68 MPKV 71

[Close]

BLAST of Lag0017154 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-16
Identity = 45/65 (69.23%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGWVVE 65
          P K+QWPELVFV+Y  AAAIIEKENP+VKA KIL+G  RI NFD  RVW D N+   VV+
Sbjct: 8  PRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVEDVVVK 67

Query: 66 TPKVG 71
          TPKVG
Sbjct: 68 TPKVG 72

[Close]

BLAST of Lag0017154 vs. NCBI nr
Match: KAE8650337.1 (hypothetical protein Csa_010581 [Cucumis sativus])

HSP 1 Score: 88.2 bits (217), Expect = 3.1e-14
Identity = 41/60 (68.33%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 8  KSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGWVVETP 67
          K++WPELVFV +  AA IIEKENP+VKA KIL  SPRI NFD +RVWVDCNI   VV+ P
Sbjct: 9  KTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEERVVKVP 68

[Close]

BLAST of Lag0017154 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.6e-11
Identity = 35/69 (50.72%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 2  SECPPGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNING 61
          S C PGKS WP LV V   VA AIIE++NPNVKA  +  G+P   +F C RV +  N  G
Sbjct: 1  SSC-PGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRG 60

Query: 62 WVVETPKVG 71
           VV  P++G
Sbjct: 61 LVVSPPRIG 68

[Close]

BLAST of Lag0017154 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 4.0e-09
Identity = 34/67 (50.75%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 3  ECPPGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGW 62
          EC PGK +WPELV      AAAIIE+ENPNV++      S    +F C RVWV  +  G 
Sbjct: 3  EC-PGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGV 62

Query: 63 VVETPKV 70
          VV TP+V
Sbjct: 63 VVRTPRV 68

[Close]

BLAST of Lag0017154 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 6.9e-09
Identity = 29/64 (45.31%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDCNINGWVVE 65
          PGK+ WPELV    ++AAA +E+EN NV A  +  GS    +F C RVWV  N +G V  
Sbjct: 5  PGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTS 64

Query: 66 TPKV 70
           P +
Sbjct: 65 VPHI 68

[Close]

BLAST of Lag0017154 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.8e-08
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 7   GKSQWPELVFVEYDVAAAIIEKENPNVKAHKI-LAGSPRILNFDCTRVWVDCNINGWVVE 66
           GK  WPEL+ V   +A  IIEKENP++    I L+GSP  L++ C RV +  NI G+VV+
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQ 106

Query: 67  TPKV 70
            P V
Sbjct: 107 MPVV 110

[Close]

BLAST of Lag0017154 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 2.2e-07
Identity = 33/71 (46.48%), Postives = 43/71 (60.56%), Query Frame = 0


Query: 2  SECPPGKSQWPELVFVEYDVAAAIIEKENPNVKAHKILAGSP--RILNFDCTRVWVDCNI 61
          S+CP  K+ WPELV    D+AA II+ EN NVKA  +  G P  + LNF+  RV+VD   
Sbjct: 3  SQCPV-KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVD--E 62

Query: 62 NGWVVETPKVG 71
          N  V + P +G
Sbjct: 63 NRVVTQVPAIG 70

The following BLAST results are available for this feature:

BLAST of Lag0017154 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN56897.1	2.1e-18	67.65	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049286.1	1.5e-16	69.84	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KAA0049288.1	1.9e-16	64.06	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049287.1	1.9e-16	69.23	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]
KAE8650337.1	3.1e-14	68.33	hypothetical protein Csa_010581 [Cucumis sativus]	[more]

BLAST of Lag0017154 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	5.6e-11	50.72	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P80211	4.0e-09	50.75	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P82381	6.9e-09	45.31	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P05118	5.8e-08	48.44	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
Q6XNP7	2.2e-07	46.48	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 8..70 e-value: 1.4E-18 score: 67.3
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 3..70
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..69 e-value: 2.3E-21 score: 77.8
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 3..70
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 5..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017154.1	Lag0017154.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity