Lag0017153 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017153
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Protease inhibitor protein
Location	chr5: 610763 .. 611077 (+)
RNA-Seq Expression	Lag0017153
Synteny	Lag0017153

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCAATTATGTCCAGGTATGTTCATTCCATCACTTAATCACTTGTGAATATGTTTAATTTGATTGTAAGTTATGTATATTAGCGCAAATTTAATATGAATTATATGTTTCTAAACAGGAAAAAGCCAGTGGCCGGAGCTTGTTTTTGTAAAATTTTCTGTTGCTGCTCTGATCATCGAAAAGGAGAATCCCAATGTCAAAGCACACCAGATTCTAGCTGGAAGTCCGAGAATTTTGAACTTCGATTGTTCACGAGTTTGGGTTGATGTCAATATAAATGGTGATGTTGTTTTAGTTCCTAAAGTTGGTTAA

mRNA sequence

ATGTCTCAATTATGTCCAGGAAAAAGCCAGTGGCCGGAGCTTGTTTTTGTAAAATTTTCTGTTGCTGCTCTGATCATCGAAAAGGAGAATCCCAATGTCAAAGCACACCAGATTCTAGCTGGAAGTCCGAGAATTTTGAACTTCGATTGTTCACGAGTTTGGGTTGATGTCAATATAAATGGTGATGTTGTTTTAGTTCCTAAAGTTGGTTAA

Coding sequence (CDS)

ATGTCTCAATTATGTCCAGGAAAAAGCCAGTGGCCGGAGCTTGTTTTTGTAAAATTTTCTGTTGCTGCTCTGATCATCGAAAAGGAGAATCCCAATGTCAAAGCACACCAGATTCTAGCTGGAAGTCCGAGAATTTTGAACTTCGATTGTTCACGAGTTTGGGTTGATGTCAATATAAATGGTGATGTTGTTTTAGTTCCTAAAGTTGGTTAA

Protein sequence

MSQLCPGKSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGDVVLVPKVG

Homology

[Close]

BLAST of Lag0017153 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 98.2 bits (243), Expect = 3.0e-17
Identity = 44/65 (67.69%), Postives = 56/65 (86.15%), Query Frame = 0


Query: 6  PGKSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGDVVL 65
          P K++WPELVFVK+S+AA IIEKENP+V+A +IL+G+PRILNFD +RVWVD+N+   VV 
Sbjct: 7  PRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEVVVK 66

Query: 66 VPKVG 71
           PKVG
Sbjct: 67 KPKVG 71

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BLAST of Lag0017153 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-16
Identity = 42/67 (62.69%), Postives = 55/67 (82.09%), Query Frame = 0


Query: 3  QLCPGKSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGD 62
          Q  P K+QWPELVF+K+S+AA IIE+ENP+V+A +IL+G+PRILNFD  RVW DVN+   
Sbjct: 5  QSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDV 64

Query: 63 VVLVPKV 70
          +V +PKV
Sbjct: 65 IVKMPKV 71

[Close]

BLAST of Lag0017153 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-15
Identity = 45/68 (66.18%), Postives = 51/68 (75.00%), Query Frame = 0


Query: 3  QLCPGKSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGD 62
          Q  P K+QWPELVFVK+S AA IIEKENP+VKA +IL+G  RI NFD  RVW DVN+   
Sbjct: 5  QSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVEDV 64

Query: 63 VVLVPKVG 71
          VV  PKVG
Sbjct: 65 VVKTPKVG 72

[Close]

BLAST of Lag0017153 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 92.4 bits (228), Expect = 1.6e-15
Identity = 45/63 (71.43%), Postives = 51/63 (80.95%), Query Frame = 0


Query: 8  KSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGDVVLVP 67
          K++WPELVFV+FS AA IIEKENP+VKA +ILA SPRI NFD SRVWVD N+   VV VP
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 68 KVG 71
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of Lag0017153 vs. NCBI nr
Match: KAE8650337.1 (hypothetical protein Csa_010581 [Cucumis sativus])

HSP 1 Score: 85.1 bits (209), Expect = 2.6e-13
Identity = 42/60 (70.00%), Postives = 46/60 (76.67%), Query Frame = 0


Query: 8  KSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGDVVLVP 67
          K++WPELVFV F  AA IIEKENP+VKA +IL  SPRI NFD SRVWVD NI   VV VP
Sbjct: 9  KTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEERVVKVP 68

[Close]

BLAST of Lag0017153 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-11
Identity = 35/66 (53.03%), Postives = 44/66 (66.67%), Query Frame = 0


Query: 5  CPGKSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGDVV 64
          CPGKS WP LV V  SVA  IIE++NPNVKA  +  G+P   +F C+RV + VN  G VV
Sbjct: 3  CPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVV 62

Query: 65 LVPKVG 71
            P++G
Sbjct: 63 SPPRIG 68

[Close]

BLAST of Lag0017153 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.1e-10
Identity = 32/68 (47.06%), Postives = 42/68 (61.76%), Query Frame = 0


Query: 2  SQLCPGKSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNING 61
          S+ CPGK+ WPELV    ++AA  +E+EN NV A  +  GS    +F C RVWV VN +G
Sbjct: 1  SRRCPGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHG 60

Query: 62 DVVLVPKV 70
           V  VP +
Sbjct: 61 VVTSVPHI 68

[Close]

BLAST of Lag0017153 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.3e-09
Identity = 34/66 (51.52%), Postives = 41/66 (62.12%), Query Frame = 0


Query: 5   CPGKSQWPELVFVKFSVAALIIEKENPNVKAHQILA-GSPRILNFDCSRVWVDVNINGDV 64
           C GK  WPEL+ V    A  IIEKENP++    IL  GSP   +F C RV + VNI GDV
Sbjct: 45  CKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDV 104

Query: 65  VLVPKV 70
           V +P+V
Sbjct: 105 VQIPRV 110

[Close]

BLAST of Lag0017153 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 6.9e-09
Identity = 32/65 (49.23%), Postives = 39/65 (60.00%), Query Frame = 0


Query: 5  CPGKSQWPELVFVKFSVAALIIEKENPNVKAHQILAGSPRILNFDCSRVWVDVNINGDVV 64
          CPGK +WPELV      AA IIE+ENPNV++      S    +F C RVWV V+  G VV
Sbjct: 4  CPGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVV 63

Query: 65 LVPKV 70
            P+V
Sbjct: 64 RTPRV 68

[Close]

BLAST of Lag0017153 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.8e-08
Identity = 32/67 (47.76%), Postives = 42/67 (62.69%), Query Frame = 0


Query: 4   LCPGKSQWPELVFVKFSVAALIIEKENPNVKAHQI-LAGSPRILNFDCSRVWVDVNINGD 63
           +C GK  WPEL+ V   +A  IIEKENP++    I L+GSP  L++ C RV +  NI G 
Sbjct: 44  MCEGKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGF 103

Query: 64  VVLVPKV 70
           VV +P V
Sbjct: 104 VVQMPVV 110

The following BLAST results are available for this feature:

BLAST of Lag0017153 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN56897.1	3.0e-17	67.69	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049288.1	1.9e-16	62.69	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049287.1	1.2e-15	66.18	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]
KAA0049286.1	1.6e-15	71.43	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KAE8650337.1	2.6e-13	70.00	hypothetical protein Csa_010581 [Cucumis sativus]	[more]

BLAST of Lag0017153 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	1.5e-11	53.03	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P82381	2.1e-10	47.06	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P16231	5.3e-09	51.52	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P80211	6.9e-09	49.23	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P05118	5.8e-08	47.76	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..69 e-value: 6.5E-22 score: 79.6
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 2..70
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 8..70 e-value: 5.6E-18 score: 65.4
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 2..70
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 4..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017153.1	Lag0017153.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity