Homology
BLAST of Lag0017147 vs. NCBI nr
Match:
XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])
HSP 1 Score: 868.2 bits (2242), Expect = 3.6e-248
Identity = 437/540 (80.93%), Postives = 470/540 (87.04%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ FF ANF PLSP+ F KRAAAVYG RPSVIYG RVFTWSETY RCL+LASALVHHF
Sbjct: 42 MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
FLP +L+S SQ S + P LVL+PA PDTPP EF DYNRVLEMRF FTP+ N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221
Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS NC P RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341
Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
VDLIVAG LPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401
Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461
Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
KSGRIEMKDRAKD+VVR NGE AVSTV+VEAVLMSHP V EAAV+ +RLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521
Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
KLKNGSRAS EEIVEFCRAHLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574
BLAST of Lag0017147 vs. NCBI nr
Match:
KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 865.1 bits (2234), Expect = 3.0e-247
Identity = 440/540 (81.48%), Postives = 471/540 (87.22%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ F ANF PLSPV F KRAAAVY RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 1 MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 61 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
FLP VLKS SQ S + P LVL+PA PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
IWAMAALGGCNICLR +TADAIF N+ELH+VTLLCGP+TLLKMI ESS N P SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
Query: 421 KSGRIEMKDRAKDLVVRANGEA-VSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
KSGRIEMKDRAKD+VVRANGEA VSTV+VEAVLMSHP V EAAVV +RLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480
Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
KLKNGSRAS EEIVEFCR HLPEFM+PERVVFGDLPMNS GKVQKF+VREKAK +NT +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533
BLAST of Lag0017147 vs. NCBI nr
Match:
KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 864.4 bits (2232), Expect = 5.2e-247
Identity = 439/540 (81.30%), Postives = 471/540 (87.22%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ F ANF PLSPV F KRAAAVY RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 1 MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 61 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
FLP VLKS SQ S + P LVL+P PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPTHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
IWAMAALGGCNICLR +TADAIF N+ELH+VTLLCGP+TLLKMI ESS N P SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE+VQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDLFTS 360
Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
KSGRIEMKDRAKD+VVRANGE AVSTV+VEAVLMSHP V EAAVV +RLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480
Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
KLKNGSRAS EEIVEFCR HLPEFM+PERVVFGDLPMNS GKVQKF+VREKAK +NT +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533
BLAST of Lag0017147 vs. NCBI nr
Match:
XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])
HSP 1 Score: 864.0 bits (2231), Expect = 6.8e-247
Identity = 439/540 (81.30%), Postives = 473/540 (87.59%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ F ANF PLSPV FLK++AAVY RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 49 MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+VSPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168
Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
FLP VL+S SQ S + P LVL+PA PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228
Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS N P LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348
Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408
Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468
Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
KSGRIEMKDRAKD+VVRANGE AVSTV+VEAVLMSHP V EAAVV +RLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528
Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
KLKNGSRAS EEIV+FCR HLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581
BLAST of Lag0017147 vs. NCBI nr
Match:
XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 862.1 bits (2226), Expect = 2.6e-246
Identity = 437/540 (80.93%), Postives = 472/540 (87.41%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ F ANF PLSP+ F ++AAVYG RPS+IYG RVFTWSETY RCL+LASALVHHF
Sbjct: 1 MDPFSPFPANFVPLSPLTFFNKSAAVYGARPSLIYGARVFTWSETYGRCLHLASALVHHF 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 61 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDS 120
Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
FLP VLKS SQ S + P LVL+PA PDTP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSVQFPALVLIPAHPDTPLSEFLDYNRVLGMRFDDFTPRTNAELD 180
Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
IWAMAALGGCNICLR +T + IF N+ELH+VTLLCGP+TLLKMI ESS N P LSRR
Sbjct: 241 IWAMAALGGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 300
Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNF EE VQFEDLITS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFGEETVQFEDLITS 360
Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
KSGRIEMKDRAKD+VVRANGE AVSTVEVEAVLMSHP V EAAV+ +RLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVI-------GERLCGFV 480
Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
KLKNGSRAS EEIVEFCRAHLPEFM+PERVVFGDLPMNS GKVQKF+VREKAKA+NT NT
Sbjct: 481 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKALNTNNT 533
BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match:
Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)
HSP 1 Score: 484.2 bits (1245), Expect = 1.9e-135
Identity = 261/555 (47.03%), Postives = 365/555 (65.77%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
+EG ANF+PLSP+ FL+R+A VY DR S+++G TW +TY+RCL LASAL +
Sbjct: 48 IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+S GD+VAA+APN+P ++ELHFAVPMAG I+ PLNT+LD +TL++LL + KI+FVD
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167
Query: 121 QFLPI---VLKSFSQNAKSFEIPTLVLV-------PADPDTP-------PFEFPDYNRVL 180
Q L I L +++ K+ + LVL+ +D D+ F++ +Y +L
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDY-EYETLL 227
Query: 181 EMRFGNF-TPKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSP 240
+ F KP E DPISIN TSG+T KGV+YSHR AYLNSLA +F + + P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287
Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI 300
V+LWTV MF CNGWC +W +AA GG NICLR ++ IF N+ +H+VT + G T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347
Query: 301 SE--SSNCTPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
+ PL RV+++ G P+ +IL K+ E GFN+S+ YG+TE GP WKP
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407
Query: 361 FDEEMVQFEDLITSLE---------IDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYK 420
+D ++ + + + +DVKDP++ME+V DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467
Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPK 480
+++AT +AF GDW+ +GD+A+++ G IE+KDR KD+++ + GE +S+VEVE VL SH
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527
Query: 481 VTEAAVVEKCEVGFRKRLCGFVKLKNG-SRASVEEIVEFCRAHLPEFMVPERVVFGDLPM 526
V EAAVV + + + + CGFVKLK G EEI+ FCR HLP +M P+ +VFGD+P
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587
BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match:
M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)
HSP 1 Score: 480.7 bits (1236), Expect = 2.1e-134
Identity = 256/546 (46.89%), Postives = 353/546 (64.65%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
MEG CSAN PLSP+ FL+R++ Y D S++YG +TW++T+ RCL LASAL HF
Sbjct: 29 MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+SPGD+VA + NIPE+YELHFAVPMAG I+ LN + D+A ++ LL + K+IFV+
Sbjct: 89 GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148
Query: 121 QFLPIVLKSFSQNA-KSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNF-TPKPNGEL 180
Q L + A K + PTLVL+ + YN +L +F +P E
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEC 208
Query: 181 DPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMFRCNGWCF 240
DPISIN TSG+T K V+YSHR AYLNS+A + G+ + V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGT--RSVYLWSVPMFHCNGWCF 268
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI---SESSNCTPLSRRV 300
W AA G NIC+R ++ AIF+N+ LH+VT T+L MI E + TPL +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328
Query: 301 DLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEMVQFE-- 360
+++ G P +++ ++ E GF +++ YG+TE GPA KP +D EE +
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKAR 388
Query: 361 ---DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
+ + E+DV+DPVSMESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448
Query: 421 TGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEKCEVGFR 480
TGD+ ++H+ G I++KDR KD+V+ + GE VSTVEVE VL SH V EAAVV + + +
Sbjct: 449 TGDLGVKHEDGYIQLKDRKKDVVI-SGGENVSTVEVETVLYSHEAVLEAAVVARPDKLWG 508
Query: 481 KRLCGFVKLKNG--SRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREK 531
+ C FV LK G + S ++I++FCR LP +M P+ VVF +LP STGK+QK++++EK
Sbjct: 509 ETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568
BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match:
M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)
HSP 1 Score: 474.9 bits (1221), Expect = 1.2e-132
Identity = 251/546 (45.97%), Postives = 352/546 (64.47%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
MEG CSAN PLSP+ FL+R++ Y D S++YG +TW++T+ RCL LASAL H
Sbjct: 29 MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+SPGD+VA + N+PE+YELHFAVPMAG I+ LN + D+A ++ LL + K+IFV+
Sbjct: 89 GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148
Query: 121 QFLPIVLKSFSQNA-KSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNF-TPKPNGEL 180
Q L + A K + PTLVL+ + YN +L +F +P E
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEW 208
Query: 181 DPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMFRCNGWCF 240
DPISIN TSG+T K V+YSHR AYLNS+A + G+ +T V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTT--SVYLWSVPMFHCNGWCF 268
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI---SESSNCTPLSRRV 300
W AA G NIC+R ++ AIF+N+ LH+VT T+L MI E + TPL +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328
Query: 301 DLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEMVQFE-- 360
+++ G P +++ ++ E GF +++ YG+TE GPA KP +D EE +
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKAR 388
Query: 361 ---DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
+ + E+DV+DPV+MESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448
Query: 421 TGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEKCEVGFR 480
+GD+ ++H+ G I++KDR KD+V+ + GE +STVEVE VL SH V EAAVV + + +
Sbjct: 449 SGDLGVKHEDGYIQLKDRKKDVVI-SGGENISTVEVETVLYSHEAVLEAAVVARPDKLWG 508
Query: 481 KRLCGFVKLKNG--SRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREK 531
+ C FV LK G + S ++I++FCR LP +M P+ VVF +LP STGK+QK++++EK
Sbjct: 509 ETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568
BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match:
F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)
HSP 1 Score: 471.5 bits (1212), Expect = 1.3e-131
Identity = 249/548 (45.44%), Postives = 352/548 (64.23%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
MEG AN+ PL+P+ FL R+A VY DR S++YG +TW +T RC+ +ASAL
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+S GD+V+ +APN+P + ELHF VPMAGA++ LN + D++ +A+LL+ K+IF D
Sbjct: 63 GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122
Query: 121 QFLPIVLKSFS-QNAKSFEIPTLVLVPADPDTPPF-------EFPDYNRVLEMRFGNF-T 180
QFL I + + K ++P LVL+P +P T E +Y V+ M +F
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182
Query: 181 PKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMF 240
+P E D IS+N TSG+T KGV+YSHR AYLNSLA + + + S SP +LWT MF
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMF 242
Query: 241 RCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI--SESSNCT 300
CNGWC +W + A+GG NICLR +TA AIF+N+ H+VT + G T+L MI + S
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302
Query: 301 PLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEM 360
PL +V I P ++ K+ E GF++ + YG+TE GP I WKP +D EE
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362
Query: 361 VQFE-----DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAF 420
+ + + + EI VKDPV+M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422
Query: 421 SGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEK 480
G W+ +GD+ ++H G IE+KDR+KD+++ + GE +S++EVE+ L +HP V EAAVV +
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVAR 482
Query: 481 CEVGFRKRLCGFVKLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFL 529
+ + + C FVKLK+GS+AS EE++ +CR LP +M P +VF DLP STGKVQKF+
Sbjct: 483 PDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFV 542
BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match:
Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)
HSP 1 Score: 414.8 bits (1065), Expect = 1.4e-114
Identity = 225/556 (40.47%), Postives = 350/556 (62.95%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ C AN PL+P+ FLKRA+ Y +R S+IYG FTW +TY RC LA++L+
Sbjct: 1 MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
N++ D+V+ +APN+P +YE+HF+VPM GA+++P+NT+LDA T+A++L+ +PKI+FVD
Sbjct: 61 NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120
Query: 121 QFLPIV--LKSFSQNAKSFEIPTLVLV-PADPDTPPFEFP-DYNRVLEMRFGNFTP---- 180
+F P++ + +S P ++L+ D T PF DY ++ R G TP
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180
Query: 181 ---KPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
+ + E DPIS+N TSG+T KGV+ SH+ AYL++L+ I + GI PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240
Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESSN 300
+ MF CNGW W++AA GG N+C+R +TA I+ N+ELH VT + T+ + + E S
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300
Query: 301 C--TPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
+P S V ++ G P ++ KV + GF++ +GYG+TEA GP + W+ +++
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360
Query: 361 -----EMVQFEDL--ITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
E+ Q + + +T ++DVK+ ++ESV DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420
Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAA 480
EAF W TGD+ + H G +E+KDR+KD+++ + GE +S++EVE VL + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480
Query: 481 VVEKCEVGFRKRLCGFVKLKNGSRASVE---EIVEFCRAHLPEFMVPERVV-FGDLPMNS 529
VV + + C FV LK G V +++++CR ++P FM P++VV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540
BLAST of Lag0017147 vs. ExPASy TrEMBL
Match:
A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)
HSP 1 Score: 868.2 bits (2242), Expect = 1.7e-248
Identity = 437/540 (80.93%), Postives = 470/540 (87.04%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ FF ANF PLSP+ F KRAAAVYG RPSVIYG RVFTWSETY RCL+LASALVHHF
Sbjct: 42 MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
FLP +L+S SQ S + P LVL+PA PDTPP EF DYNRVLEMRF FTP+ N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221
Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS NC P RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341
Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
VDLIVAG LPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401
Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461
Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
KSGRIEMKDRAKD+VVR NGE AVSTV+VEAVLMSHP V EAAV+ +RLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521
Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
KLKNGSRAS EEIVEFCRAHLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574
BLAST of Lag0017147 vs. ExPASy TrEMBL
Match:
A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)
HSP 1 Score: 864.0 bits (2231), Expect = 3.3e-247
Identity = 439/540 (81.30%), Postives = 473/540 (87.59%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ F ANF PLSPV FLK++AAVY RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 49 MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+VSPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168
Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
FLP VL+S SQ S + P LVL+PA PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228
Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288
Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS N P LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348
Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408
Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468
Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
KSGRIEMKDRAKD+VVRANGE AVSTV+VEAVLMSHP V EAAVV +RLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528
Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
KLKNGSRAS EEIV+FCR HLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581
BLAST of Lag0017147 vs. ExPASy TrEMBL
Match:
A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)
HSP 1 Score: 812.4 bits (2097), Expect = 1.1e-231
Identity = 419/540 (77.59%), Postives = 464/540 (85.93%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
MEGF HC NFAPLSPV FLKRA+ +YG RPS++YG RVFTWS+TY RCL++ASALVHHF
Sbjct: 1 MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
++SP DLV AMAPNIPELYELHFAVPMAG IIS LNTKLD+ TL+LLLQQL PK+IF+DS
Sbjct: 61 HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
Query: 121 QFLPIVLKSFSQNAKSFEIPTLVLVPADPDTP-PFEFPDYNRVLEMRFG--NFTPKPNGE 180
QFLPI+LKS S + P L+L+P+DP+T P +F DYN++L MR G NFTP+PN E
Sbjct: 121 QFLPILLKSLEN--ISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAE 180
Query: 181 LDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGW 240
LDPISIN TSGSTGLHKGVIYSHRAAYLNSLA IFRS IC ST SPVFLWTVDMFRCNGW
Sbjct: 181 LDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGW 240
Query: 241 CFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS--NCTP--LS 300
CFIW MAALGGCNICLRT+TADAIF NVELHRVTLLCGP+TLLKMI ESS NC P LS
Sbjct: 241 CFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLS 300
Query: 301 RRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEEMVQFED- 360
RRVDLIVAG LPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D
Sbjct: 301 RRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDH 360
Query: 361 LITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGD 420
LITSLEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTGD
Sbjct: 361 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGD 420
Query: 421 VAIRHKSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKR 480
V +RHKSGRIEMKDRAKD+VVR +GE AVSTVEVE VLMSHP V E AVV + +
Sbjct: 421 VGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------RT 480
Query: 481 LCGFVKLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAI 529
L GFVKLKNGS+ + +EIVEFCR HLPEFMVP+ +VFGDLPMNSTGKVQKF++REKAKA+
Sbjct: 481 LYGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 532
BLAST of Lag0017147 vs. ExPASy TrEMBL
Match:
A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)
HSP 1 Score: 718.4 bits (1853), Expect = 2.2e-203
Identity = 373/468 (79.70%), Postives = 405/468 (86.54%), Query Frame = 0
Query: 71 MAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDSQFLPIVLKSF 130
MAPNIPELYELHFAVPMAGAIIS LNTKLD+ TL+LLLQQL PK+IF+DSQFLPI+LKS
Sbjct: 1 MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60
Query: 131 SQNAKSFEIPTLVLVPADPDTP-PFEFPDYNRVLEMRFG--NFTPKPNGELDPISINCTS 190
+ S + P LVL+P+DPDTP P EF DYN+VL MRFG +FTP+PN ELDPISIN TS
Sbjct: 61 ENS--SIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTS 120
Query: 191 GSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCFIWAMAALG 250
GSTGLHKGVIYSHRAAYLNSLA IFRS IC ST SPVFLWTVDMFRCNGWCFIW MAALG
Sbjct: 121 GSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALG 180
Query: 251 GCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS--NCTP--LSRRVDLIVAGV 310
GCNICLRT+TADAIF NVELHRVTLLCGP TLLKMI ESS NC P LSRRVDLIVAG
Sbjct: 181 GCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAGA 240
Query: 311 LPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITSLEIDVKDP 370
LPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP F+E+ VQFEDLITSLEIDVKDP
Sbjct: 241 LPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKDP 300
Query: 371 VSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGRIEM 430
+SMESVL DGETLGEVMLRGNTLMSGYYKN+KAT EAF G+ WYRTGDV +RHKSGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEM 360
Query: 431 KDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFVKLKNGSR 490
KDRAKD+VVR +GE AVSTVEVE VLMSHP V EAAVV + + L GFVKLKN S+
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGE------RTLYGFVKLKNRSK 420
Query: 491 ASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAI 529
+ +EIVEFCR HLPEFM+P+R+VFGDLPMNSTGKVQKF +REK KA+
Sbjct: 421 ENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKAL 460
BLAST of Lag0017147 vs. ExPASy TrEMBL
Match:
A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)
HSP 1 Score: 701.0 bits (1808), Expect = 3.7e-198
Identity = 368/470 (78.30%), Postives = 404/470 (85.96%), Query Frame = 0
Query: 71 MAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDSQFLPIVLKSF 130
MAPNIPELYELHFAVPMAG IIS LNTKLD+ TL+LLLQQL PK+IF+DSQFLPI+LKS
Sbjct: 1 MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60
Query: 131 SQNAKSFEIPTLVLVPADPDTP-PFEFPDYNRVLEMRFG--NFTPKPNGELDPISINCTS 190
S + P L+L+P+DP+T P +F DYN++L MR G NFTP+PN ELDPISIN TS
Sbjct: 61 EN--ISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTS 120
Query: 191 GSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCFIWAMAALG 250
GSTGLHKGVIYSHRAAYLNSLA IFRS IC ST SPVFLWTVDMFRCNGWCFIW MAALG
Sbjct: 121 GSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALG 180
Query: 251 GCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS--NCTP--LSRRVDLIVAGV 310
GCNICLRT+TADAIF NVELHRVTLLCGP+TLLKMI ESS NC P LSRRVDLIVAG
Sbjct: 181 GCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGA 240
Query: 311 LPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEEMVQFED-LITSLEIDVK 370
LPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D LITSLEIDVK
Sbjct: 241 LPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVK 300
Query: 371 DPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGRI 430
DP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTGDV +RHKSGRI
Sbjct: 301 DPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRI 360
Query: 431 EMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFVKLKNG 490
EMKDRAKD+VVR +GE AVSTVEVE VLMSHP V E AVV + + L GFVKLKNG
Sbjct: 361 EMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------RTLYGFVKLKNG 420
Query: 491 SRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAI 529
S+ + +EIVEFCR HLPEFMVP+ +VFGDLPMNSTGKVQKF++REKAKA+
Sbjct: 421 SKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 462
BLAST of Lag0017147 vs. TAIR 10
Match:
AT2G17650.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-136
Identity = 261/555 (47.03%), Postives = 365/555 (65.77%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
+EG ANF+PLSP+ FL+R+A VY DR S+++G TW +TY+RCL LASAL +
Sbjct: 48 IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+S GD+VAA+APN+P ++ELHFAVPMAG I+ PLNT+LD +TL++LL + KI+FVD
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167
Query: 121 QFLPI---VLKSFSQNAKSFEIPTLVLV-------PADPDTP-------PFEFPDYNRVL 180
Q L I L +++ K+ + LVL+ +D D+ F++ +Y +L
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDY-EYETLL 227
Query: 181 EMRFGNF-TPKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSP 240
+ F KP E DPISIN TSG+T KGV+YSHR AYLNSLA +F + + P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287
Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI 300
V+LWTV MF CNGWC +W +AA GG NICLR ++ IF N+ +H+VT + G T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347
Query: 301 SE--SSNCTPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
+ PL RV+++ G P+ +IL K+ E GFN+S+ YG+TE GP WKP
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407
Query: 361 FDEEMVQFEDLITSLE---------IDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYK 420
+D ++ + + + +DVKDP++ME+V DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467
Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPK 480
+++AT +AF GDW+ +GD+A+++ G IE+KDR KD+++ + GE +S+VEVE VL SH
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527
Query: 481 VTEAAVVEKCEVGFRKRLCGFVKLKNG-SRASVEEIVEFCRAHLPEFMVPERVVFGDLPM 526
V EAAVV + + + + CGFVKLK G EEI+ FCR HLP +M P+ +VFGD+P
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587
BLAST of Lag0017147 vs. TAIR 10
Match:
AT1G20560.1 (acyl activating enzyme 1 )
HSP 1 Score: 471.5 bits (1212), Expect = 9.1e-133
Identity = 249/548 (45.44%), Postives = 352/548 (64.23%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
MEG AN+ PL+P+ FL R+A VY DR S++YG +TW +T RC+ +ASAL
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
+S GD+V+ +APN+P + ELHF VPMAGA++ LN + D++ +A+LL+ K+IF D
Sbjct: 63 GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122
Query: 121 QFLPIVLKSFS-QNAKSFEIPTLVLVPADPDTPPF-------EFPDYNRVLEMRFGNF-T 180
QFL I + + K ++P LVL+P +P T E +Y V+ M +F
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182
Query: 181 PKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMF 240
+P E D IS+N TSG+T KGV+YSHR AYLNSLA + + + S SP +LWT MF
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMF 242
Query: 241 RCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI--SESSNCT 300
CNGWC +W + A+GG NICLR +TA AIF+N+ H+VT + G T+L MI + S
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302
Query: 301 PLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEM 360
PL +V I P ++ K+ E GF++ + YG+TE GP I WKP +D EE
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362
Query: 361 VQFE-----DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAF 420
+ + + + EI VKDPV+M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422
Query: 421 SGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEK 480
G W+ +GD+ ++H G IE+KDR+KD+++ + GE +S++EVE+ L +HP V EAAVV +
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVAR 482
Query: 481 CEVGFRKRLCGFVKLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFL 529
+ + + C FVKLK+GS+AS EE++ +CR LP +M P +VF DLP STGKVQKF+
Sbjct: 483 PDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFV 542
BLAST of Lag0017147 vs. TAIR 10
Match:
AT1G66120.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 414.8 bits (1065), Expect = 1.0e-115
Identity = 225/556 (40.47%), Postives = 350/556 (62.95%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ C AN PL+P+ FLKRA+ Y +R S+IYG FTW +TY RC LA++L+
Sbjct: 1 MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
N++ D+V+ +APN+P +YE+HF+VPM GA+++P+NT+LDA T+A++L+ +PKI+FVD
Sbjct: 61 NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120
Query: 121 QFLPIV--LKSFSQNAKSFEIPTLVLV-PADPDTPPFEFP-DYNRVLEMRFGNFTP---- 180
+F P++ + +S P ++L+ D T PF DY ++ R G TP
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180
Query: 181 ---KPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
+ + E DPIS+N TSG+T KGV+ SH+ AYL++L+ I + GI PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240
Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESSN 300
+ MF CNGW W++AA GG N+C+R +TA I+ N+ELH VT + T+ + + E S
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300
Query: 301 C--TPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
+P S V ++ G P ++ KV + GF++ +GYG+TEA GP + W+ +++
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360
Query: 361 -----EMVQFEDL--ITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
E+ Q + + +T ++DVK+ ++ESV DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420
Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAA 480
EAF W TGD+ + H G +E+KDR+KD+++ + GE +S++EVE VL + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480
Query: 481 VVEKCEVGFRKRLCGFVKLKNGSRASVE---EIVEFCRAHLPEFMVPERVV-FGDLPMNS 529
VV + + C FV LK G V +++++CR ++P FM P++VV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540
BLAST of Lag0017147 vs. TAIR 10
Match:
AT1G65880.1 (benzoyloxyglucosinolate 1 )
HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-111
Identity = 223/564 (39.54%), Postives = 331/564 (58.69%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ C AN PL+P+ FLKRA+ Y +R S+IYG FTW +TY RC LA++L+
Sbjct: 1 MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
N+S D+V+ MAPN P LYE+HFAVPMAGA+++P+NT+LDA ++A +L+ +PKI+F+D
Sbjct: 61 NISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDR 120
Query: 121 QFLPIVLKS---FSQNAKSFEIPTLVLVPAD-PDTPPFEFPDYNRVLEMRFGNFTP---- 180
F + +S S + +P + + D P FE DY +++ G TP
Sbjct: 121 SFEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQR--GEPTPSMVA 180
Query: 181 ---KPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
+ E DPIS+N TSG+T KGV+ SHR AYL +L+ I + G C PV+LWT
Sbjct: 181 RMFRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTC----PVYLWT 240
Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI--SES 300
+ MF CNGW F W AA GG ++C+R +TA I+ N+E+H VT +C T+ ++ S
Sbjct: 241 LPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNS 300
Query: 301 SNCTPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
+ +P S V ++ G P ++ KV GF + + YG TEA GP + W+ ++
Sbjct: 301 LDLSPRSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLP 360
Query: 361 EMVQFE-------DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
E Q E ++ ++DVK+ + +S DG+T+GE++++G+++M GY KN KAT
Sbjct: 361 ENQQMELKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKAT 420
Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAA 480
EAF W TGDV + H G +E+KDR+KD+++ + GE +S+VEVE VL +PKV E A
Sbjct: 421 FEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIII-SGGENISSVEVENVLYKYPKVLETA 480
Query: 481 VVEKCEVGFRKRLCGFVKLKNGSRASVEE-----------IVEFCRAHLPEFMVPERVVF 529
VV + + C FV L+ E+ ++E+CR +LP FM P +VVF
Sbjct: 481 VVAMPHPTWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVF 540
BLAST of Lag0017147 vs. TAIR 10
Match:
AT1G65890.1 (acyl activating enzyme 12 )
HSP 1 Score: 398.7 bits (1023), Expect = 7.5e-111
Identity = 220/564 (39.01%), Postives = 334/564 (59.22%), Query Frame = 0
Query: 1 MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
M+ C AN PL+P+ FLKRA+ Y +R S+IYG FTW +TY RC LA++L+
Sbjct: 1 MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60
Query: 61 NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
N+ D+V+ +APN P +YE+HFAVPMAGA+++P+NT+LDA ++A +L+ +PKI+F+
Sbjct: 61 NIGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYR 120
Query: 121 QFLPI---VLKSFSQNAKSFEIPTLVLVPAD-PDTPPFEFPDYNRVLEMRFGNFTPK--- 180
F P+ +L+ S + +P + + D P E DY +++ G TP
Sbjct: 121 SFEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQR--GEPTPLLLA 180
Query: 181 ----PNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
E DPIS+N TSG+T KGV+ SHR AYL++L+ I + G C PV+LWT
Sbjct: 181 RMFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTC----PVYLWT 240
Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESSN 300
+ MF CNGW F W AA GG ++C+R +TA I+ N+E+H VT +C T+ ++ + N
Sbjct: 241 LPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLK-GN 300
Query: 301 CTPLSRR---VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE- 360
LS R V ++ G P ++ KV GF + + YG+TEA GP + W+ ++
Sbjct: 301 SLDLSHRSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRL 360
Query: 361 -EMVQFE-------DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKA 420
E Q E ++ E+DV++ + ESV DG+T+GE++++G+++M GY KN KA
Sbjct: 361 PENQQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKA 420
Query: 421 TCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEA 480
T EAF W +GDV + H G +E+KDR+KD+++ + GE +S+VEVE ++ +PKV E
Sbjct: 421 TYEAFKHGWLNSGDVGVIHPDGHVEIKDRSKDIII-SGGENISSVEVENIIYKYPKVLET 480
Query: 481 AVVEKCEVGFRKRLCGFVKLKNGSRASVE----------EIVEFCRAHLPEFMVPERVVF 529
AVV + + C FV L+ G + + +++E+CR +LP FM P +VVF
Sbjct: 481 AVVAMPHPTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVF 540
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_023007650.1 | 3.6e-248 | 80.93 | probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima] | [more] |
| KAG7015844.1 | 3.0e-247 | 81.48 | putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma ... | [more] |
| KAG6577804.1 | 5.2e-247 | 81.30 | putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma ... | [more] |
| XP_022923197.1 | 6.8e-247 | 81.30 | probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata] | [more] |
| XP_023552791.1 | 2.6e-246 | 80.93 | probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9SEY5 | 1.9e-135 | 47.03 | Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1 | [more] |
| M4IS92 | 2.1e-134 | 46.89 | Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1 | [more] |
| M4IRL4 | 1.2e-132 | 45.97 | Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1 | [more] |
| F4HUK6 | 1.3e-131 | 45.44 | Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1 | [more] |
| Q9C8D4 | 1.4e-114 | 40.47 | Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1L3K3 | 1.7e-248 | 80.93 | probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... | [more] |
| A0A6J1E5I3 | 3.3e-247 | 81.30 | probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=... | [more] |
| A0A1S3BKP8 | 1.1e-231 | 77.59 | probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 S... | [more] |
| A0A0A0L3L0 | 2.2e-203 | 79.70 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1 | [more] |
| A0A5A7UEI8 | 3.7e-198 | 78.30 | Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... | [more] |
