Lag0017147 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0017147 vs. NCBI nr
Match: XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 868.2 bits (2242), Expect = 3.6e-248
Identity = 437/540 (80.93%), Postives = 470/540 (87.04%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+ FF   ANF PLSP+ F KRAAAVYG RPSVIYG RVFTWSETY RCL+LASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           ++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
            FLP +L+S SQ    S + P LVL+PA PDTPP EF DYNRVLEMRF  FTP+ N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221

Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
           IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS   NC P   RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341

Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
           VDLIVAG LPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401

Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461

Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
           KSGRIEMKDRAKD+VVR NGE AVSTV+VEAVLMSHP V EAAV+        +RLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521

Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
           KLKNGSRAS EEIVEFCRAHLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574

BLAST of Lag0017147 vs. NCBI nr
Match: KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 865.1 bits (2234), Expect = 3.0e-247
Identity = 440/540 (81.48%), Postives = 471/540 (87.22%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+ F    ANF PLSPV F KRAAAVY  RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           ++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
            FLP VLKS SQ    S + P LVL+PA PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
           IWAMAALGGCNICLR +TADAIF N+ELH+VTLLCGP+TLLKMI ESS   N  P  SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300

Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
           VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360

Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDLVVRANGEA-VSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
           KSGRIEMKDRAKD+VVRANGEA VSTV+VEAVLMSHP V EAAVV        +RLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480

Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
           KLKNGSRAS EEIVEFCR HLPEFM+PERVVFGDLPMNS GKVQKF+VREKAK +NT +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533

BLAST of Lag0017147 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 864.4 bits (2232), Expect = 5.2e-247
Identity = 439/540 (81.30%), Postives = 471/540 (87.22%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+ F    ANF PLSPV F KRAAAVY  RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           ++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
            FLP VLKS SQ    S + P LVL+P  PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPTHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
           IWAMAALGGCNICLR +TADAIF N+ELH+VTLLCGP+TLLKMI ESS   N  P  SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300

Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
           VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE+VQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDLFTS 360

Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
           KSGRIEMKDRAKD+VVRANGE AVSTV+VEAVLMSHP V EAAVV        +RLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480

Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
           KLKNGSRAS EEIVEFCR HLPEFM+PERVVFGDLPMNS GKVQKF+VREKAK +NT +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533

BLAST of Lag0017147 vs. NCBI nr
Match: XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 864.0 bits (2231), Expect = 6.8e-247
Identity = 439/540 (81.30%), Postives = 473/540 (87.59%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+ F    ANF PLSPV FLK++AAVY  RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           +VSPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
            FLP VL+S SQ    S + P LVL+PA PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228

Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
           IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS   N  P LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348

Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
           VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408

Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468

Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
           KSGRIEMKDRAKD+VVRANGE AVSTV+VEAVLMSHP V EAAVV        +RLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528

Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
           KLKNGSRAS EEIV+FCR HLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581

BLAST of Lag0017147 vs. NCBI nr
Match: XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.1 bits (2226), Expect = 2.6e-246
Identity = 437/540 (80.93%), Postives = 472/540 (87.41%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+ F    ANF PLSP+ F  ++AAVYG RPS+IYG RVFTWSETY RCL+LASALVHHF
Sbjct: 1   MDPFSPFPANFVPLSPLTFFNKSAAVYGARPSLIYGARVFTWSETYGRCLHLASALVHHF 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           ++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDS 120

Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
            FLP VLKS SQ    S + P LVL+PA PDTP  EF DYNRVL MRF +FTP+ N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSVQFPALVLIPAHPDTPLSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
           IWAMAALGGCNICLR +T + IF N+ELH+VTLLCGP+TLLKMI ESS   N  P LSRR
Sbjct: 241 IWAMAALGGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 300

Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
           VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNF EE VQFEDLITS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFGEETVQFEDLITS 360

Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
           KSGRIEMKDRAKD+VVRANGE AVSTVEVEAVLMSHP V EAAV+        +RLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVI-------GERLCGFV 480

Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
           KLKNGSRAS EEIVEFCRAHLPEFM+PERVVFGDLPMNS GKVQKF+VREKAKA+NT NT
Sbjct: 481 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKALNTNNT 533

BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.9e-135
Identity = 261/555 (47.03%), Postives = 365/555 (65.77%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           +EG     ANF+PLSP+ FL+R+A VY DR S+++G    TW +TY+RCL LASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
            +S GD+VAA+APN+P ++ELHFAVPMAG I+ PLNT+LD +TL++LL   + KI+FVD 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPI---VLKSFSQNAKSFEIPTLVLV-------PADPDTP-------PFEFPDYNRVL 180
           Q L I    L   +++ K+ +   LVL+        +D D+         F++ +Y  +L
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDY-EYETLL 227

Query: 181 EMRFGNF-TPKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSP 240
           +     F   KP  E DPISIN TSG+T   KGV+YSHR AYLNSLA +F   +  +  P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR ++   IF N+ +H+VT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SE--SSNCTPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
                +   PL  RV+++  G  P+ +IL K+ E GFN+S+ YG+TE  GP     WKP 
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407

Query: 361 FDEEMVQFEDLITSLE---------IDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYK 420
           +D   ++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467

Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPK 480
           +++AT +AF GDW+ +GD+A+++  G IE+KDR KD+++ + GE +S+VEVE VL SH  
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527

Query: 481 VTEAAVVEKCEVGFRKRLCGFVKLKNG-SRASVEEIVEFCRAHLPEFMVPERVVFGDLPM 526
           V EAAVV + +  + +  CGFVKLK G      EEI+ FCR HLP +M P+ +VFGD+P 
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587

BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 2.1e-134
Identity = 256/546 (46.89%), Postives = 353/546 (64.65%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           MEG   CSAN  PLSP+ FL+R++  Y D  S++YG   +TW++T+ RCL LASAL  HF
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
            +SPGD+VA  + NIPE+YELHFAVPMAG I+  LN + D+A ++ LL   + K+IFV+ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLPIVLKSFSQNA-KSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNF-TPKPNGEL 180
           Q L     +    A K  + PTLVL+          +  YN +L     +F   +P  E 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEC 208

Query: 181 DPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMFRCNGWCF 240
           DPISIN TSG+T   K V+YSHR AYLNS+A +   G+ +    V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGT--RSVYLWSVPMFHCNGWCF 268

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI---SESSNCTPLSRRV 300
            W  AA G  NIC+R ++  AIF+N+ LH+VT      T+L MI    E +  TPL  +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328

Query: 301 DLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEMVQFE-- 360
           +++  G  P  +++ ++ E GF +++ YG+TE  GPA     KP +D    EE    +  
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKAR 388

Query: 361 ---DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
              + +   E+DV+DPVSMESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448

Query: 421 TGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEKCEVGFR 480
           TGD+ ++H+ G I++KDR KD+V+ + GE VSTVEVE VL SH  V EAAVV + +  + 
Sbjct: 449 TGDLGVKHEDGYIQLKDRKKDVVI-SGGENVSTVEVETVLYSHEAVLEAAVVARPDKLWG 508

Query: 481 KRLCGFVKLKNG--SRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREK 531
           +  C FV LK G  +  S ++I++FCR  LP +M P+ VVF +LP  STGK+QK++++EK
Sbjct: 509 ETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568

BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.2e-132
Identity = 251/546 (45.97%), Postives = 352/546 (64.47%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           MEG   CSAN  PLSP+ FL+R++  Y D  S++YG   +TW++T+ RCL LASAL  H 
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
            +SPGD+VA  + N+PE+YELHFAVPMAG I+  LN + D+A ++ LL   + K+IFV+ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLPIVLKSFSQNA-KSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNF-TPKPNGEL 180
           Q L     +    A K  + PTLVL+          +  YN +L     +F   +P  E 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEW 208

Query: 181 DPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMFRCNGWCF 240
           DPISIN TSG+T   K V+YSHR AYLNS+A +   G+ +T   V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTT--SVYLWSVPMFHCNGWCF 268

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI---SESSNCTPLSRRV 300
            W  AA G  NIC+R ++  AIF+N+ LH+VT      T+L MI    E +  TPL  +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328

Query: 301 DLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEMVQFE-- 360
           +++  G  P  +++ ++ E GF +++ YG+TE  GPA     KP +D    EE    +  
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKAR 388

Query: 361 ---DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
              + +   E+DV+DPV+MESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448

Query: 421 TGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEKCEVGFR 480
           +GD+ ++H+ G I++KDR KD+V+ + GE +STVEVE VL SH  V EAAVV + +  + 
Sbjct: 449 SGDLGVKHEDGYIQLKDRKKDVVI-SGGENISTVEVETVLYSHEAVLEAAVVARPDKLWG 508

Query: 481 KRLCGFVKLKNG--SRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREK 531
           +  C FV LK G  +  S ++I++FCR  LP +M P+ VVF +LP  STGK+QK++++EK
Sbjct: 509 ETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568

BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 1.3e-131
Identity = 249/548 (45.44%), Postives = 352/548 (64.23%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           MEG     AN+ PL+P+ FL R+A VY DR S++YG   +TW +T  RC+ +ASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
            +S GD+V+ +APN+P + ELHF VPMAGA++  LN + D++ +A+LL+    K+IF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPIVLKSFS-QNAKSFEIPTLVLVPADPDTPPF-------EFPDYNRVLEMRFGNF-T 180
           QFL I   +    + K  ++P LVL+P +P T          E  +Y  V+ M   +F  
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 181 PKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMF 240
            +P  E D IS+N TSG+T   KGV+YSHR AYLNSLA +  + + S  SP +LWT  MF
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMF 242

Query: 241 RCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI--SESSNCT 300
            CNGWC +W + A+GG NICLR +TA AIF+N+  H+VT + G  T+L MI  +  S   
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302

Query: 301 PLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEM 360
           PL  +V  I     P   ++ K+ E GF++ + YG+TE  GP  I  WKP +D    EE 
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362

Query: 361 VQFE-----DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAF 420
            + +     + +   EI VKDPV+M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422

Query: 421 SGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEK 480
            G W+ +GD+ ++H  G IE+KDR+KD+++ + GE +S++EVE+ L +HP V EAAVV +
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVAR 482

Query: 481 CEVGFRKRLCGFVKLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFL 529
            +  + +  C FVKLK+GS+AS EE++ +CR  LP +M P  +VF DLP  STGKVQKF+
Sbjct: 483 PDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFV 542

BLAST of Lag0017147 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.4e-114
Identity = 225/556 (40.47%), Postives = 350/556 (62.95%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+    C AN  PL+P+ FLKRA+  Y +R S+IYG   FTW +TY RC  LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           N++  D+V+ +APN+P +YE+HF+VPM GA+++P+NT+LDA T+A++L+  +PKI+FVD 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPIV--LKSFSQNAKSFEIPTLVLV-PADPDTPPFEFP-DYNRVLEMRFGNFTP---- 180
           +F P++  +       +S   P ++L+   D  T PF    DY  ++  R G  TP    
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180

Query: 181 ---KPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
              + + E DPIS+N TSG+T   KGV+ SH+ AYL++L+ I  +  GI     PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240

Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESSN 300
           + MF CNGW   W++AA GG N+C+R +TA  I+ N+ELH VT +    T+ + + E S 
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300

Query: 301 C--TPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
              +P S  V ++  G  P   ++ KV + GF++ +GYG+TEA GP +   W+  +++  
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360

Query: 361 -----EMVQFEDL--ITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
                E+ Q + +  +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420

Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAA 480
            EAF   W  TGD+ + H  G +E+KDR+KD+++ + GE +S++EVE VL  + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480

Query: 481 VVEKCEVGFRKRLCGFVKLKNGSRASVE---EIVEFCRAHLPEFMVPERVV-FGDLPMNS 529
           VV      + +  C FV LK G    V    +++++CR ++P FM P++VV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540

BLAST of Lag0017147 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.7e-248
Identity = 437/540 (80.93%), Postives = 470/540 (87.04%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+ FF   ANF PLSP+ F KRAAAVYG RPSVIYG RVFTWSETY RCL+LASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           ++SPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
            FLP +L+S SQ    S + P LVL+PA PDTPP EF DYNRVLEMRF  FTP+ N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221

Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
           IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS   NC P   RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341

Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
           VDLIVAG LPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401

Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461

Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
           KSGRIEMKDRAKD+VVR NGE AVSTV+VEAVLMSHP V EAAV+        +RLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521

Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
           KLKNGSRAS EEIVEFCRAHLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574

BLAST of Lag0017147 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.3e-247
Identity = 439/540 (81.30%), Postives = 473/540 (87.59%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+ F    ANF PLSPV FLK++AAVY  RPSVIYG RVFTWSETY R L+LASALVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           +VSPGD+VAAMAPN+PELYELHFAVPMAGAIISPLNTKLDA TL+LLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 QFLPIVLKSFSQN-AKSFEIPTLVLVPADPDTPPFEFPDYNRVLEMRFGNFTPKPNGELD 180
            FLP VL+S SQ    S + P LVL+PA PDTPP EF DYNRVL MRF +FTP+ N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228

Query: 181 PISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKGV+YSHRAAYLNSLA IFRSGIC +T SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288

Query: 241 IWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS---NCTP-LSRR 300
           IWAMAALGGCNICLR +T DAIF N+ELH+VTLLCGP+TLLKMI ESS   N  P LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348

Query: 301 VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITS 360
           VDLIVAG LPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408

Query: 361 LEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468

Query: 421 KSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFV 480
           KSGRIEMKDRAKD+VVRANGE AVSTV+VEAVLMSHP V EAAVV        +RLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528

Query: 481 KLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAINTYNT 534
           KLKNGSRAS EEIV+FCR HLPEFM+PERVVFGDLPMNS GKVQKF+ REKAKA+NT +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581

BLAST of Lag0017147 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 1.1e-231
Identity = 419/540 (77.59%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           MEGF HC  NFAPLSPV FLKRA+ +YG RPS++YG RVFTWS+TY RCL++ASALVHHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           ++SP DLV AMAPNIPELYELHFAVPMAG IIS LNTKLD+ TL+LLLQQL PK+IF+DS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QFLPIVLKSFSQNAKSFEIPTLVLVPADPDTP-PFEFPDYNRVLEMRFG--NFTPKPNGE 180
           QFLPI+LKS      S + P L+L+P+DP+T  P +F DYN++L MR G  NFTP+PN E
Sbjct: 121 QFLPILLKSLEN--ISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAE 180

Query: 181 LDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGW 240
           LDPISIN TSGSTGLHKGVIYSHRAAYLNSLA IFRS IC ST SPVFLWTVDMFRCNGW
Sbjct: 181 LDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGW 240

Query: 241 CFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS--NCTP--LS 300
           CFIW MAALGGCNICLRT+TADAIF NVELHRVTLLCGP+TLLKMI ESS  NC P  LS
Sbjct: 241 CFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLS 300

Query: 301 RRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEEMVQFED- 360
           RRVDLIVAG LPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D 
Sbjct: 301 RRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDH 360

Query: 361 LITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGD 420
           LITSLEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTGD
Sbjct: 361 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGD 420

Query: 421 VAIRHKSGRIEMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKR 480
           V +RHKSGRIEMKDRAKD+VVR +GE AVSTVEVE VLMSHP V E AVV +      + 
Sbjct: 421 VGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------RT 480

Query: 481 LCGFVKLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAI 529
           L GFVKLKNGS+ + +EIVEFCR HLPEFMVP+ +VFGDLPMNSTGKVQKF++REKAKA+
Sbjct: 481 LYGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 532

BLAST of Lag0017147 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 2.2e-203
Identity = 373/468 (79.70%), Postives = 405/468 (86.54%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDSQFLPIVLKSF 130
           MAPNIPELYELHFAVPMAGAIIS LNTKLD+ TL+LLLQQL PK+IF+DSQFLPI+LKS 
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 131 SQNAKSFEIPTLVLVPADPDTP-PFEFPDYNRVLEMRFG--NFTPKPNGELDPISINCTS 190
             +  S + P LVL+P+DPDTP P EF DYN+VL MRFG  +FTP+PN ELDPISIN TS
Sbjct: 61  ENS--SIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTS 120

Query: 191 GSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCFIWAMAALG 250
           GSTGLHKGVIYSHRAAYLNSLA IFRS IC ST SPVFLWTVDMFRCNGWCFIW MAALG
Sbjct: 121 GSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALG 180

Query: 251 GCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS--NCTP--LSRRVDLIVAGV 310
           GCNICLRT+TADAIF NVELHRVTLLCGP TLLKMI ESS  NC P  LSRRVDLIVAG 
Sbjct: 181 GCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAGA 240

Query: 311 LPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEEMVQFEDLITSLEIDVKDP 370
           LPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP F+E+ VQFEDLITSLEIDVKDP
Sbjct: 241 LPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKDP 300

Query: 371 VSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGRIEM 430
           +SMESVL DGETLGEVMLRGNTLMSGYYKN+KAT EAF G+ WYRTGDV +RHKSGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEM 360

Query: 431 KDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFVKLKNGSR 490
           KDRAKD+VVR +GE AVSTVEVE VLMSHP V EAAVV +      + L GFVKLKN S+
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGE------RTLYGFVKLKNRSK 420

Query: 491 ASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAI 529
            + +EIVEFCR HLPEFM+P+R+VFGDLPMNSTGKVQKF +REK KA+
Sbjct: 421 ENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKAL 460

BLAST of Lag0017147 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 3.7e-198
Identity = 368/470 (78.30%), Postives = 404/470 (85.96%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDSQFLPIVLKSF 130
           MAPNIPELYELHFAVPMAG IIS LNTKLD+ TL+LLLQQL PK+IF+DSQFLPI+LKS 
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 SQNAKSFEIPTLVLVPADPDTP-PFEFPDYNRVLEMRFG--NFTPKPNGELDPISINCTS 190
                S + P L+L+P+DP+T  P +F DYN++L MR G  NFTP+PN ELDPISIN TS
Sbjct: 61  EN--ISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTS 120

Query: 191 GSTGLHKGVIYSHRAAYLNSLAMIFRSGIC-STPSPVFLWTVDMFRCNGWCFIWAMAALG 250
           GSTGLHKGVIYSHRAAYLNSLA IFRS IC ST SPVFLWTVDMFRCNGWCFIW MAALG
Sbjct: 121 GSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALG 180

Query: 251 GCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESS--NCTP--LSRRVDLIVAGV 310
           GCNICLRT+TADAIF NVELHRVTLLCGP+TLLKMI ESS  NC P  LSRRVDLIVAG 
Sbjct: 181 GCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGA 240

Query: 311 LPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEEMVQFED-LITSLEIDVK 370
           LPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D LITSLEIDVK
Sbjct: 241 LPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVK 300

Query: 371 DPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGRI 430
           DP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTGDV +RHKSGRI
Sbjct: 301 DPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRI 360

Query: 431 EMKDRAKDLVVRANGE-AVSTVEVEAVLMSHPKVTEAAVVEKCEVGFRKRLCGFVKLKNG 490
           EMKDRAKD+VVR +GE AVSTVEVE VLMSHP V E AVV +      + L GFVKLKNG
Sbjct: 361 EMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------RTLYGFVKLKNG 420

Query: 491 SRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFLVREKAKAI 529
           S+ + +EIVEFCR HLPEFMVP+ +VFGDLPMNSTGKVQKF++REKAKA+
Sbjct: 421 SKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 462

BLAST of Lag0017147 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-136
Identity = 261/555 (47.03%), Postives = 365/555 (65.77%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           +EG     ANF+PLSP+ FL+R+A VY DR S+++G    TW +TY+RCL LASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
            +S GD+VAA+APN+P ++ELHFAVPMAG I+ PLNT+LD +TL++LL   + KI+FVD 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPI---VLKSFSQNAKSFEIPTLVLV-------PADPDTP-------PFEFPDYNRVL 180
           Q L I    L   +++ K+ +   LVL+        +D D+         F++ +Y  +L
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDY-EYETLL 227

Query: 181 EMRFGNF-TPKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSP 240
           +     F   KP  E DPISIN TSG+T   KGV+YSHR AYLNSLA +F   +  +  P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR ++   IF N+ +H+VT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SE--SSNCTPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
                +   PL  RV+++  G  P+ +IL K+ E GFN+S+ YG+TE  GP     WKP 
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407

Query: 361 FDEEMVQFEDLITSLE---------IDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYK 420
           +D   ++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467

Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPK 480
           +++AT +AF GDW+ +GD+A+++  G IE+KDR KD+++ + GE +S+VEVE VL SH  
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527

Query: 481 VTEAAVVEKCEVGFRKRLCGFVKLKNG-SRASVEEIVEFCRAHLPEFMVPERVVFGDLPM 526
           V EAAVV + +  + +  CGFVKLK G      EEI+ FCR HLP +M P+ +VFGD+P 
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587

BLAST of Lag0017147 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 471.5 bits (1212), Expect = 9.1e-133
Identity = 249/548 (45.44%), Postives = 352/548 (64.23%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           MEG     AN+ PL+P+ FL R+A VY DR S++YG   +TW +T  RC+ +ASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
            +S GD+V+ +APN+P + ELHF VPMAGA++  LN + D++ +A+LL+    K+IF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPIVLKSFS-QNAKSFEIPTLVLVPADPDTPPF-------EFPDYNRVLEMRFGNF-T 180
           QFL I   +    + K  ++P LVL+P +P T          E  +Y  V+ M   +F  
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 181 PKPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMIFRSGICSTPSPVFLWTVDMF 240
            +P  E D IS+N TSG+T   KGV+YSHR AYLNSLA +  + + S  SP +LWT  MF
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMF 242

Query: 241 RCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI--SESSNCT 300
            CNGWC +W + A+GG NICLR +TA AIF+N+  H+VT + G  T+L MI  +  S   
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302

Query: 301 PLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EEM 360
           PL  +V  I     P   ++ K+ E GF++ + YG+TE  GP  I  WKP +D    EE 
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362

Query: 361 VQFE-----DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAF 420
            + +     + +   EI VKDPV+M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422

Query: 421 SGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAAVVEK 480
            G W+ +GD+ ++H  G IE+KDR+KD+++ + GE +S++EVE+ L +HP V EAAVV +
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVAR 482

Query: 481 CEVGFRKRLCGFVKLKNGSRASVEEIVEFCRAHLPEFMVPERVVFGDLPMNSTGKVQKFL 529
            +  + +  C FVKLK+GS+AS EE++ +CR  LP +M P  +VF DLP  STGKVQKF+
Sbjct: 483 PDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFV 542

BLAST of Lag0017147 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 414.8 bits (1065), Expect = 1.0e-115
Identity = 225/556 (40.47%), Postives = 350/556 (62.95%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+    C AN  PL+P+ FLKRA+  Y +R S+IYG   FTW +TY RC  LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           N++  D+V+ +APN+P +YE+HF+VPM GA+++P+NT+LDA T+A++L+  +PKI+FVD 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPIV--LKSFSQNAKSFEIPTLVLV-PADPDTPPFEFP-DYNRVLEMRFGNFTP---- 180
           +F P++  +       +S   P ++L+   D  T PF    DY  ++  R G  TP    
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180

Query: 181 ---KPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
              + + E DPIS+N TSG+T   KGV+ SH+ AYL++L+ I  +  GI     PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240

Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESSN 300
           + MF CNGW   W++AA GG N+C+R +TA  I+ N+ELH VT +    T+ + + E S 
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300

Query: 301 C--TPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
              +P S  V ++  G  P   ++ KV + GF++ +GYG+TEA GP +   W+  +++  
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360

Query: 361 -----EMVQFEDL--ITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
                E+ Q + +  +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420

Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAA 480
            EAF   W  TGD+ + H  G +E+KDR+KD+++ + GE +S++EVE VL  + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480

Query: 481 VVEKCEVGFRKRLCGFVKLKNGSRASVE---EIVEFCRAHLPEFMVPERVV-FGDLPMNS 529
           VV      + +  C FV LK G    V    +++++CR ++P FM P++VV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540

BLAST of Lag0017147 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-111
Identity = 223/564 (39.54%), Postives = 331/564 (58.69%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+    C AN  PL+P+ FLKRA+  Y +R S+IYG   FTW +TY RC  LA++L+   
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           N+S  D+V+ MAPN P LYE+HFAVPMAGA+++P+NT+LDA ++A +L+  +PKI+F+D 
Sbjct: 61  NISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDR 120

Query: 121 QFLPIVLKS---FSQNAKSFEIPTLVLVPAD-PDTPPFEFPDYNRVLEMRFGNFTP---- 180
            F  +  +S    S    +  +P + +   D P    FE  DY  +++   G  TP    
Sbjct: 121 SFEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQR--GEPTPSMVA 180

Query: 181 ---KPNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
              +   E DPIS+N TSG+T   KGV+ SHR AYL +L+ I  +  G C    PV+LWT
Sbjct: 181 RMFRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTC----PVYLWT 240

Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMI--SES 300
           + MF CNGW F W  AA GG ++C+R +TA  I+ N+E+H VT +C   T+  ++    S
Sbjct: 241 LPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNS 300

Query: 301 SNCTPLSRRVDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
            + +P S  V ++  G  P   ++ KV   GF + + YG TEA GP +   W+  ++   
Sbjct: 301 LDLSPRSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLP 360

Query: 361 EMVQFE-------DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
           E  Q E        ++   ++DVK+  + +S   DG+T+GE++++G+++M GY KN KAT
Sbjct: 361 ENQQMELKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKAT 420

Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEAA 480
            EAF   W  TGDV + H  G +E+KDR+KD+++ + GE +S+VEVE VL  +PKV E A
Sbjct: 421 FEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIII-SGGENISSVEVENVLYKYPKVLETA 480

Query: 481 VVEKCEVGFRKRLCGFVKLKNGSRASVEE-----------IVEFCRAHLPEFMVPERVVF 529
           VV      + +  C FV L+       E+           ++E+CR +LP FM P +VVF
Sbjct: 481 VVAMPHPTWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVF 540

BLAST of Lag0017147 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 398.7 bits (1023), Expect = 7.5e-111
Identity = 220/564 (39.01%), Postives = 334/564 (59.22%), Query Frame = 0

Query: 1   MEGFFHCSANFAPLSPVGFLKRAAAVYGDRPSVIYGGRVFTWSETYRRCLNLASALVHHF 60
           M+    C AN  PL+P+ FLKRA+  Y +R S+IYG   FTW +TY RC  LA++L+   
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60

Query: 61  NVSPGDLVAAMAPNIPELYELHFAVPMAGAIISPLNTKLDAATLALLLQQLQPKIIFVDS 120
           N+   D+V+ +APN P +YE+HFAVPMAGA+++P+NT+LDA ++A +L+  +PKI+F+  
Sbjct: 61  NIGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYR 120

Query: 121 QFLPI---VLKSFSQNAKSFEIPTLVLVPAD-PDTPPFEFPDYNRVLEMRFGNFTPK--- 180
            F P+   +L+  S    +  +P + +   D P     E  DY  +++   G  TP    
Sbjct: 121 SFEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQR--GEPTPLLLA 180

Query: 181 ----PNGELDPISINCTSGSTGLHKGVIYSHRAAYLNSLAMI--FRSGICSTPSPVFLWT 240
                  E DPIS+N TSG+T   KGV+ SHR AYL++L+ I  +  G C    PV+LWT
Sbjct: 181 RMFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTC----PVYLWT 240

Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTITADAIFNNVELHRVTLLCGPTTLLKMISESSN 300
           + MF CNGW F W  AA GG ++C+R +TA  I+ N+E+H VT +C   T+  ++ +  N
Sbjct: 241 LPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLK-GN 300

Query: 301 CTPLSRR---VDLIVAGVLPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE- 360
              LS R   V ++  G  P   ++ KV   GF + + YG+TEA GP +   W+  ++  
Sbjct: 301 SLDLSHRSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRL 360

Query: 361 -EMVQFE-------DLITSLEIDVKDPVSMESVLADGETLGEVMLRGNTLMSGYYKNLKA 420
            E  Q E        ++   E+DV++  + ESV  DG+T+GE++++G+++M GY KN KA
Sbjct: 361 PENQQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKA 420

Query: 421 TCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDLVVRANGEAVSTVEVEAVLMSHPKVTEA 480
           T EAF   W  +GDV + H  G +E+KDR+KD+++ + GE +S+VEVE ++  +PKV E 
Sbjct: 421 TYEAFKHGWLNSGDVGVIHPDGHVEIKDRSKDIII-SGGENISSVEVENIIYKYPKVLET 480

Query: 481 AVVEKCEVGFRKRLCGFVKLKNGSRASVE----------EIVEFCRAHLPEFMVPERVVF 529
           AVV      + +  C FV L+ G   + +          +++E+CR +LP FM P +VVF
Sbjct: 481 AVVAMPHPTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVF 540