Lag0017133 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017133
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDirigent protein
Locationchr5: 423276 .. 423836 (-)
RNA-Seq ExpressionLag0017133
SyntenyLag0017133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCAATCCTCTCTAATTCTCTCTTCCTTTCTCACAATCATTCTCTCTTCTCTCTTGGCTCCGACCGCCGCCGCTGAGACCGCTTACCATCATCACCACCGCCATTGCCACCTCCGATCCCTCCACTTTTCCCTCTTCCAACACGAAACGATCAACAAAACTGGCTACATCATCGTGTCGGGAGTGGCTGGCCCCGGTGTGAGCCAAACCACCACCCCATTTGGCACCATGTTTGTTTTTCAAGACCCTTTGACCACCACTGCCGACCGAGCATCAAAGCTGGTCGGCACGTCGGAGGGAACCTCCATAACCTCAGGCCTCGATGGTTTGCGAAGCATTAGCATAGCCAAGATTAGCTTGCGTTTGAGGCACCATACAGGTTCTCTCTCGATCGTTGGTGGGACACATAATGTTAAACCGTCGAATCACCCGATCGTCGGAGGGACCGGAGATTTCTTGTTCGTGCAAGGCTATGTGACGTCGTCTCCAGTGGATCTCATCGGCATCACCGTGGTTTACAAGTTGGAGTTTCATCTTTACTGGCCGCCCTATGCATAA

mRNA sequence

ATGCCTCAATCCTCTCTAATTCTCTCTTCCTTTCTCACAATCATTCTCTCTTCTCTCTTGGCTCCGACCGCCGCCGCTGAGACCGCTTACCATCATCACCACCGCCATTGCCACCTCCGATCCCTCCACTTTTCCCTCTTCCAACACGAAACGATCAACAAAACTGGCTACATCATCGTGTCGGGAGTGGCTGGCCCCGGTGTGAGCCAAACCACCACCCCATTTGGCACCATGTTTGTTTTTCAAGACCCTTTGACCACCACTGCCGACCGAGCATCAAAGCTGGTCGGCACGTCGGAGGGAACCTCCATAACCTCAGGCCTCGATGGTTTGCGAAGCATTAGCATAGCCAAGATTAGCTTGCGTTTGAGGCACCATACAGGTTCTCTCTCGATCGTTGGTGGGACACATAATGTTAAACCGTCGAATCACCCGATCGTCGGAGGGACCGGAGATTTCTTGTTCGTGCAAGGCTATGTGACGTCGTCTCCAGTGGATCTCATCGGCATCACCGTGGTTTACAAGTTGGAGTTTCATCTTTACTGGCCGCCCTATGCATAA

Coding sequence (CDS)

ATGCCTCAATCCTCTCTAATTCTCTCTTCCTTTCTCACAATCATTCTCTCTTCTCTCTTGGCTCCGACCGCCGCCGCTGAGACCGCTTACCATCATCACCACCGCCATTGCCACCTCCGATCCCTCCACTTTTCCCTCTTCCAACACGAAACGATCAACAAAACTGGCTACATCATCGTGTCGGGAGTGGCTGGCCCCGGTGTGAGCCAAACCACCACCCCATTTGGCACCATGTTTGTTTTTCAAGACCCTTTGACCACCACTGCCGACCGAGCATCAAAGCTGGTCGGCACGTCGGAGGGAACCTCCATAACCTCAGGCCTCGATGGTTTGCGAAGCATTAGCATAGCCAAGATTAGCTTGCGTTTGAGGCACCATACAGGTTCTCTCTCGATCGTTGGTGGGACACATAATGTTAAACCGTCGAATCACCCGATCGTCGGAGGGACCGGAGATTTCTTGTTCGTGCAAGGCTATGTGACGTCGTCTCCAGTGGATCTCATCGGCATCACCGTGGTTTACAAGTTGGAGTTTCATCTTTACTGGCCGCCCTATGCATAA

Protein sequence

MPQSSLILSSFLTIILSSLLAPTAAAETAYHHHHRHCHLRSLHFSLFQHETINKTGYIIVSGVAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKISLRLRHHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFHLYWPPYA
Homology
BLAST of Lag0017133 vs. NCBI nr
Match: KAG7015856.1 (Dirigent protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 311.6 bits (797), Expect = 4.5e-81
Identity = 161/187 (86.10%), Postives = 168/187 (89.84%), Query Frame = 0

Query: 2   PQSSLILSSFLTIILSSLLAPTAAAE--TAYHHHHRHCHLRSLHFSLFQHETINKTGYII 61
           P  SLILSS  T+ILSSLL+  A AE   A+H HH H HLRSLHFSLFQHETINKTGY I
Sbjct: 5   PPPSLILSSLFTLILSSLLSSPALAEPNAAHHPHHPHRHLRSLHFSLFQHETINKTGYFI 64

Query: 62  VSGVAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKI 121
           V GVAGPGVSQTTTPFGTMFVF DPLTT  DRASKLVGTSEGTSITS LDGLRS+SIAKI
Sbjct: 65  VPGVAGPGVSQTTTPFGTMFVFHDPLTTAPDRASKLVGTSEGTSITSSLDGLRSLSIAKI 124

Query: 122 SLRLRHHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFH 181
           SLRLR+HTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDL+GITVVYKLEFH
Sbjct: 125 SLRLRNHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLVGITVVYKLEFH 184

Query: 182 LYWPPYA 187
           +YWPPYA
Sbjct: 185 IYWPPYA 191

BLAST of Lag0017133 vs. NCBI nr
Match: KAG6577817.1 (Dirigent protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 311.6 bits (797), Expect = 4.5e-81
Identity = 161/187 (86.10%), Postives = 168/187 (89.84%), Query Frame = 0

Query: 2   PQSSLILSSFLTIILSSLLAPTAAAE--TAYHHHHRHCHLRSLHFSLFQHETINKTGYII 61
           P  SLILSS  T+ILSSLL+  A AE   A+H HH H HLRSLHFSLFQHETINKTGY I
Sbjct: 19  PPPSLILSSLFTLILSSLLSSPALAEPNAAHHPHHPHRHLRSLHFSLFQHETINKTGYFI 78

Query: 62  VSGVAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKI 121
           V GVAGPGVSQTTTPFGTMFVF DPLTT  DRASKLVGTSEGTSITS LDGLRS+SIAKI
Sbjct: 79  VPGVAGPGVSQTTTPFGTMFVFHDPLTTAPDRASKLVGTSEGTSITSSLDGLRSLSIAKI 138

Query: 122 SLRLRHHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFH 181
           SLRLR+HTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDL+GITVVYKLEFH
Sbjct: 139 SLRLRNHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLVGITVVYKLEFH 198

Query: 182 LYWPPYA 187
           +YWPPYA
Sbjct: 199 IYWPPYA 205

BLAST of Lag0017133 vs. NCBI nr
Match: XP_023007642.1 (dirigent protein 19-like [Cucurbita maxima])

HSP 1 Score: 308.1 bits (788), Expect = 5.0e-80
Identity = 161/184 (87.50%), Postives = 166/184 (90.22%), Query Frame = 0

Query: 5   SLILSSFLTIILSSLLAPTAAAETAYHHH--HRHCHLRSLHFSLFQHETINKTGYIIVSG 64
           SLILSS  T+ILSSLL+  A AE    HH  H H HLRSLHFSLFQHETINKTGY IV G
Sbjct: 4   SLILSSLFTLILSSLLSSPALAEPNATHHPRHPHRHLRSLHFSLFQHETINKTGYFIVPG 63

Query: 65  VAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKISLR 124
           VAGPGVSQTTTPFGTMFVF DPLTT  DRASKLVGTSEGTSITSGLDGLRSISIAKISLR
Sbjct: 64  VAGPGVSQTTTPFGTMFVFHDPLTTAPDRASKLVGTSEGTSITSGLDGLRSISIAKISLR 123

Query: 125 LRHHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFHLYW 184
           LR+HTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDL+GITVVYKLEFH+YW
Sbjct: 124 LRNHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLVGITVVYKLEFHIYW 183

Query: 185 PPYA 187
           PPYA
Sbjct: 184 PPYA 187

BLAST of Lag0017133 vs. NCBI nr
Match: XP_023551883.1 (dirigent protein 11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 307.4 bits (786), Expect = 8.6e-80
Identity = 161/193 (83.42%), Postives = 169/193 (87.56%), Query Frame = 0

Query: 2   PQSSLILSSFLTIILSSLLAPTAAAE-TAYHH-------HHRHCHLRSLHFSLFQHETIN 61
           P  SLILS+  T+ILSSLL+  A AE  A HH       HH H HLRSLHFSLFQHETIN
Sbjct: 19  PPPSLILSTLFTLILSSLLSSPALAEPNAAHHPHHPHHPHHPHLHLRSLHFSLFQHETIN 78

Query: 62  KTGYIIVSGVAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRS 121
           KTGY IV GVAGPGVSQTTTPFGTMFVF+DPLTT  DRASKLVGTSEGTSITS LDGLRS
Sbjct: 79  KTGYFIVPGVAGPGVSQTTTPFGTMFVFRDPLTTAPDRASKLVGTSEGTSITSSLDGLRS 138

Query: 122 ISIAKISLRLRHHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVV 181
           +SIAKISLRLR+HTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDL+GITVV
Sbjct: 139 LSIAKISLRLRNHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLVGITVV 198

Query: 182 YKLEFHLYWPPYA 187
           YKLEFH+YWPPYA
Sbjct: 199 YKLEFHIYWPPYA 211

BLAST of Lag0017133 vs. NCBI nr
Match: XP_022923245.1 (dirigent protein 19-like [Cucurbita moschata])

HSP 1 Score: 307.0 bits (785), Expect = 1.1e-79
Identity = 162/186 (87.10%), Postives = 167/186 (89.78%), Query Frame = 0

Query: 2   PQSSLILSSFLTIILSSLLAPTAAAE-TAYHHHHRHCHLRSLHFSLFQHETINKTGYIIV 61
           P  SLILSS  T+ILSSLL+  A AE  A HH HR  HLRSLHFSLFQHETINKTGY IV
Sbjct: 20  PPPSLILSSLFTLILSSLLSSPALAEPNAAHHPHR--HLRSLHFSLFQHETINKTGYFIV 79

Query: 62  SGVAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKIS 121
            GVAGPGVSQTTTPFGTMFVF DPLTT  DRASKLVGTSEGTSITS LDGLRS+SIAKIS
Sbjct: 80  PGVAGPGVSQTTTPFGTMFVFHDPLTTAPDRASKLVGTSEGTSITSSLDGLRSLSIAKIS 139

Query: 122 LRLRHHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFHL 181
           LRLR+HTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFH+
Sbjct: 140 LRLRNHTGSLSIVGGTHNVKPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFHI 199

Query: 182 YWPPYA 187
           YWPPYA
Sbjct: 200 YWPPYA 203

BLAST of Lag0017133 vs. ExPASy Swiss-Prot
Match: Q9C523 (Dirigent protein 19 OS=Arabidopsis thaliana OX=3702 GN=DIR19 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 5.0e-06
Identity = 45/160 (28.12%), Postives = 77/160 (48.12%), Query Frame = 0

Query: 6   LILSSFLTIILSSLLAPTAAAETAYHHHHRHCHLRSLHFSLFQHETINKTGYIIVSGVAG 65
           LI S  L ++L S+   T   E+ + HH +    +  HF ++ H+ +       VS +  
Sbjct: 9   LISSRTLALVLISVTGET--LESNFLHHKKE---KLTHFRVYWHDIVTGQDSSSVSIMNP 68

Query: 66  PGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKISLRLRH 125
           P      T FG M +  +PLT T   +SK+VG ++G    +  + +  +     ++    
Sbjct: 69  PKKYTGATGFGLMRMIDNPLTLTPKLSSKMVGRAQGFYAGTSKEEIGLLMAMNFAILDGK 128

Query: 126 HTGSLSIVGGTHNV--KPSNHPIVGGTGDFLFVQGYVTSS 164
           + GS   V G ++V  K    P++GG+G F F +GYV +S
Sbjct: 129 YNGSTITVLGRNSVFDKVREMPVIGGSGLFRFARGYVQAS 163

BLAST of Lag0017133 vs. ExPASy Swiss-Prot
Match: Q9FIG6 (Dirigent protein 1 OS=Arabidopsis thaliana OX=3702 GN=DIR1 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.2e-05
Identity = 45/183 (24.59%), Postives = 81/183 (44.26%), Query Frame = 0

Query: 1   MPQSSLILSSFLTIILSSLLAPTAAAETAYHHHHRHCHLRSLHFSLFQHETINKTGYIIV 60
           M +  L+L   L+ IL   ++ TA + T  +  ++      LHF  + H+ I+      V
Sbjct: 1   MAKRFLLLLPLLSSILLLAVSVTAYSTTTPYQGYKPEKFTHLHF--YFHDVISGDKPTAV 60

Query: 61  SGVAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKIS 120
                   +     FG + +  DPLT   D +SK VG ++G   ++ +  +    +    
Sbjct: 61  KVAEARPTTTLNVKFGVIMIADDPLTEGPDPSSKEVGRAQGMYASTAMKDIVFTMVFNYV 120

Query: 121 LRLRHHTGSLSIVGGTHNV--KPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEF 180
                  GS   V G +++  K    PI+GGTG F F +GY       ++G+  V  +E+
Sbjct: 121 FTAGEFNGSTIAVYGRNDIFSKVRELPIIGGTGAFRFARGYALPKTYKIVGLDAV--VEY 179

Query: 181 HLY 182
           +++
Sbjct: 181 NVF 179

BLAST of Lag0017133 vs. ExPASy Swiss-Prot
Match: Q84TH6 (Dirigent protein 23 OS=Arabidopsis thaliana OX=3702 GN=DIR23 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 7.2e-05
Identity = 36/119 (30.25%), Postives = 56/119 (47.06%), Query Frame = 0

Query: 43  HFSLFQHETINKTGYIIVSGVAGPGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGT 102
           +   + H+T++      V    G    ++ T FG +F+  D LT TAD  SKLVG ++G 
Sbjct: 44  NLQFYFHDTLSGKNPTAVKVAQGTDTEKSPTLFGAVFMVDDALTETADPKSKLVGRAQGL 103

Query: 103 SITSGLDGLRSISIAKISLRLRHHTGS-LSIVGGTHNVKPSNH-PIVGGTGDFLFVQGY 160
             +S  + +  I           +  S +S++G    + P    PIVGGTG F   +GY
Sbjct: 104 YGSSCKEEVGLIMAMSFCFEDGPYKDSTISMIGKNSAMNPIREMPIVGGTGMFRMARGY 162

BLAST of Lag0017133 vs. ExPASy Swiss-Prot
Match: Q9FIG7 (Dirigent protein 2 OS=Arabidopsis thaliana OX=3702 GN=DIR2 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-04
Identity = 40/178 (22.47%), Postives = 77/178 (43.26%), Query Frame = 0

Query: 6   LILSSFLTIILSSLLAPTAAAETAYHHHHRHCHLRSLHFSLFQHETINKTGYIIVSGVAG 65
           L+L    TI+L S+    + A +       +   +  H   + H+ I+      V     
Sbjct: 7   LLLPLLSTILLLSVSVTESEAYSTTKPCQGYKPDKFTHLHFYFHDVISGDKPTAVKVAEA 66

Query: 66  PGVSQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKISLRLRH 125
              + +   FG + +  DPLT   D +SK VG ++G    + +  +    +  ++     
Sbjct: 67  RRTNSSNVNFGVIMIADDPLTEGPDPSSKEVGRAQGMYALTAMKNISFTMVFNLAFTAGE 126

Query: 126 HTGSLSIVGGTHNV--KPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFHLY 182
             GS   + G + +  K    PI+GGTG F F +GY  +    ++G+  V  +E++++
Sbjct: 127 FNGSTVAMYGRNEIFSKVREMPIIGGTGAFRFARGYAQAKTYKVVGLDAV--VEYNVF 182

BLAST of Lag0017133 vs. ExPASy Swiss-Prot
Match: Q67YM6 (Dirigent protein 11 OS=Arabidopsis thaliana OX=3702 GN=DIR11 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.9e-04
Identity = 39/175 (22.29%), Postives = 79/175 (45.14%), Query Frame = 0

Query: 11  FLTIILSSLLAPTAA--AETAYHHHHRHCHLRSLHFSLFQHETINKTGYIIVSGVAGPGV 70
           F T++L ++    +   ++T     ++   L  LHF  + H+ I+      +     PG 
Sbjct: 20  FSTVLLLTITVTQSKPYSKTTPFQGNKPDKLTHLHF--YFHDIISGDKPTTIRVAEAPGT 79

Query: 71  SQTTTPFGTMFVFQDPLTTTADRASKLVGTSEGTSITSGLDGLRSISIAKISLRLRHHTG 130
           + + T FG + +   P+T   + +SK VG ++G   ++ +       +           G
Sbjct: 80  NSSATVFGAVLIVDAPVTEGPELSSKEVGRAQGLYASTDMKTFGFTMVFNFVFTEGEFNG 139

Query: 131 SLSIVGGTHNV--KPSNHPIVGGTGDFLFVQGYVTSSPVDLIGITVVYKLEFHLY 182
           S + + G + +  +    PI+GGTGDF F +GY       ++ I  V  +E++++
Sbjct: 140 STAALYGRNPILLEERELPIIGGTGDFRFARGYALPKTYKVVNIDAV--VEYNVF 190

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015856.14.5e-8186.10Dirigent protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6577817.14.5e-8186.10Dirigent protein 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023007642.15.0e-8087.50dirigent protein 19-like [Cucurbita maxima][more]
XP_023551883.18.6e-8083.42dirigent protein 11-like [Cucurbita pepo subsp. pepo][more]
XP_022923245.11.1e-7987.10dirigent protein 19-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9C5235.0e-0628.13Dirigent protein 19 OS=Arabidopsis thaliana OX=3702 GN=DIR19 PE=2 SV=1[more]
Q9FIG63.2e-0524.59Dirigent protein 1 OS=Arabidopsis thaliana OX=3702 GN=DIR1 PE=2 SV=1[more]
Q84TH67.2e-0530.25Dirigent protein 23 OS=Arabidopsis thaliana OX=3702 GN=DIR23 PE=2 SV=1[more]
Q9FIG71.2e-0422.47Dirigent protein 2 OS=Arabidopsis thaliana OX=3702 GN=DIR2 PE=2 SV=1[more]
Q67YM67.9e-0422.29Dirigent protein 11 OS=Arabidopsis thaliana OX=3702 GN=DIR11 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004265Dirigent proteinPFAMPF03018Dirigentcoord: 43..177
e-value: 8.6E-26
score: 90.5
IPR044859Allene oxide cyclase/Dirigent proteinGENE3D2.40.480.10coord: 25..180
e-value: 2.3E-11
score: 45.5
NoneNo IPR availablePANTHERPTHR47586DIRIGENT PROTEINcoord: 10..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017133.1Lag0017133.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0048046 apoplast