Lag0017113 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017113
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	3-hydroxy-3-methylglutaryl-coenzyme A reductase
Location	chr5: 310151 .. 310546 (-)
RNA-Seq Expression	Lag0017113
Synteny	Lag0017113

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATGGTATCCTCCAGAAGAACCCAGCCAAGAGCCAACAATATCTTGAAACTGAAAGTCTGACTGACATGCATGCCACGTTAACCGTAGTTAATTTTGGATGGGATGGGTCAAGGTCGACTAACTCAATTGTTTGCTTCCAGGTGGGTACGGTTGGGGGTGGGACTCAACTGGCATCGCAGTCAGCTTGTCTGGACCTACTTGGAGTGAGGGGAGCCAGCAAGGAAGCCCCAGGCTCCAACTCTAGGCTGCTCGCAACCATAGTGGCTGGCTCTGTCCTGGCTGGGGAACTTTCGCTTATGTCGGCTCTGGCGGCTGGGCAGCTTGTAAAGAGTCACATGAAGTACAATAGGTCTACCAATGATGTCTCTAAATACTTACCAAGGCCTACTTGA

mRNA sequence

ATGCATGGTATCCTCCAGAAGAACCCAGCCAAGAGCCAACAATATCTTGAAACTGAAAGTCTGACTGACATGCATGCCACGTTAACCGTAGTTAATTTTGGATGGGATGGGTCAAGGTCGACTAACTCAATTGTTTGCTTCCAGGTGGGTACGGTTGGGGGTGGGACTCAACTGGCATCGCAGTCAGCTTGTCTGGACCTACTTGGAGTGAGGGGAGCCAGCAAGGAAGCCCCAGGCTCCAACTCTAGGCTGCTCGCAACCATAGTGGCTGGCTCTGTCCTGGCTGGGGAACTTTCGCTTATGTCGGCTCTGGCGGCTGGGCAGCTTGTAAAGAGTCACATGAAGTACAATAGGTCTACCAATGATGTCTCTAAATACTTACCAAGGCCTACTTGA

Coding sequence (CDS)

ATGCATGGTATCCTCCAGAAGAACCCAGCCAAGAGCCAACAATATCTTGAAACTGAAAGTCTGACTGACATGCATGCCACGTTAACCGTAGTTAATTTTGGATGGGATGGGTCAAGGTCGACTAACTCAATTGTTTGCTTCCAGGTGGGTACGGTTGGGGGTGGGACTCAACTGGCATCGCAGTCAGCTTGTCTGGACCTACTTGGAGTGAGGGGAGCCAGCAAGGAAGCCCCAGGCTCCAACTCTAGGCTGCTCGCAACCATAGTGGCTGGCTCTGTCCTGGCTGGGGAACTTTCGCTTATGTCGGCTCTGGCGGCTGGGCAGCTTGTAAAGAGTCACATGAAGTACAATAGGTCTACCAATGATGTCTCTAAATACTTACCAAGGCCTACTTGA

Protein sequence

MHGILQKNPAKSQQYLETESLTDMHATLTVVNFGWDGSRSTNSIVCFQVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALAAGQLVKSHMKYNRSTNDVSKYLPRPT

Homology

[Close]

BLAST of Lag0017113 vs. NCBI nr
Match: RVW78829.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 [Vitis vinifera])

HSP 1 Score: 137.5 bits (345), Expect = 8.3e-29
Identity = 72/80 (90.00%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 46  CFQVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALA 105
           C QVGTVGGGTQLASQSACL+LLGV+GASKE+PGSNSRLLATIVAGSVLAGELSLMSA+A
Sbjct: 445 CMQVGTVGGGTQLASQSACLNLLGVKGASKESPGSNSRLLATIVAGSVLAGELSLMSAIA 504

Query: 106 AGQLVKSHMKYNRSTNDVSK 126
           AGQLVKSH+KYNRS+ DVSK
Sbjct: 505 AGQLVKSHLKYNRSSRDVSK 524

[Close]

BLAST of Lag0017113 vs. NCBI nr
Match: XP_027919759.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Vigna unguiculata] >QCD82184.1 hydroxymethylglutaryl-CoA reductase [Vigna unguiculata])

HSP 1 Score: 135.2 bits (339), Expect = 4.1e-28
Identity = 71/81 (87.65%), Postives = 77/81 (95.06%), Query Frame = 0


Query: 48  QVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALAAG 107
           +VGTVGGGTQLASQSACL+LLGV+GASKE PG+NSRLLATIVAGSVLAGELSLMSA+AAG
Sbjct: 513 EVGTVGGGTQLASQSACLNLLGVKGASKECPGANSRLLATIVAGSVLAGELSLMSAIAAG 572

Query: 108 QLVKSHMKYNRSTNDVSKYLP 129
           QLVKSHMKYNRS  D+SK LP
Sbjct: 573 QLVKSHMKYNRSNRDMSKILP 593

[Close]

BLAST of Lag0017113 vs. NCBI nr
Match: XP_010266284.1 (PREDICTED: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Nelumbo nucifera])

HSP 1 Score: 135.2 bits (339), Expect = 4.1e-28
Identity = 77/98 (78.57%), Postives = 85/98 (86.73%), Query Frame = 0


Query: 28  LTVVNFGWDGSRSTNSIVCFQVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLAT 87
           +  VN G D   S  S+   +VGTVGGGTQLASQSACL+LLGV+GASKE+PGSNSRLLAT
Sbjct: 509 MEAVNDGKDLHISV-SMPSIEVGTVGGGTQLASQSACLNLLGVKGASKESPGSNSRLLAT 568

Query: 88  IVAGSVLAGELSLMSALAAGQLVKSHMKYNRSTNDVSK 126
           +VAGSVLAGELSLMSALAAGQLVKSHMKYNRS+ DVSK
Sbjct: 569 VVAGSVLAGELSLMSALAAGQLVKSHMKYNRSSKDVSK 605

[Close]

BLAST of Lag0017113 vs. NCBI nr
Match: XP_030539496.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Rhodamnia argentea])

HSP 1 Score: 134.8 bits (338), Expect = 5.3e-28
Identity = 71/78 (91.03%), Postives = 77/78 (98.72%), Query Frame = 0


Query: 48  QVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALAAG 107
           +VGTVGGGTQLASQSACL+LLGV+GA++EAPGSNSRLLAT+VAGSVLAGELSLMSALAAG
Sbjct: 516 EVGTVGGGTQLASQSACLNLLGVKGANREAPGSNSRLLATVVAGSVLAGELSLMSALAAG 575

Query: 108 QLVKSHMKYNRSTNDVSK 126
           QLVKSHMKYNRST DVSK
Sbjct: 576 QLVKSHMKYNRSTKDVSK 593

[Close]

BLAST of Lag0017113 vs. NCBI nr
Match: PIA56946.1 (hypothetical protein AQUCO_00700952v1 [Aquilegia coerulea])

HSP 1 Score: 134.8 bits (338), Expect = 5.3e-28
Identity = 77/98 (78.57%), Postives = 85/98 (86.73%), Query Frame = 0


Query: 28  LTVVNFGWDGSRSTNSIVCFQVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLAT 87
           +  VN G D   S  S+   +VGTVGGGTQLASQSACL+LLGV+GASKEAPGSNSRLLA+
Sbjct: 498 MEAVNDGRDLHVSV-SMPSIEVGTVGGGTQLASQSACLNLLGVKGASKEAPGSNSRLLAS 557

Query: 88  IVAGSVLAGELSLMSALAAGQLVKSHMKYNRSTNDVSK 126
           IVAGSVLAGELSLMSALAAGQLVKSHMKYNRS+ D+SK
Sbjct: 558 IVAGSVLAGELSLMSALAAGQLVKSHMKYNRSSKDISK 594

[Close]

BLAST of Lag0017113 vs. ExPASy Swiss-Prot
Match: A0A0A1C930 (3-hydroxy-3-methylglutaryl coenzyme A reductase 2-A OS=Panax ginseng OX=4054 GN=HMGR2 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 5.9e-30
Identity = 69/78 (88.46%), Postives = 77/78 (98.72%), Query Frame = 0


Query: 48  QVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALAAG 107
           +VGTVGGGTQLASQSACL+LLGV+GASKE+PGSNSRLLA+IVAGSVLAGELSLMSALAAG
Sbjct: 514 EVGTVGGGTQLASQSACLNLLGVKGASKESPGSNSRLLASIVAGSVLAGELSLMSALAAG 573

Query: 108 QLVKSHMKYNRSTNDVSK 126
           QLVKSHMKYNRS+ D++K
Sbjct: 574 QLVKSHMKYNRSSKDITK 591

[Close]

BLAST of Lag0017113 vs. ExPASy Swiss-Prot
Match: P48021 (3-hydroxy-3-methylglutaryl-coenzyme A reductase OS=Camptotheca acuminata OX=16922 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-29
Identity = 69/78 (88.46%), Postives = 75/78 (96.15%), Query Frame = 0


Query: 48  QVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALAAG 107
           +VGTVGGGTQLASQSACL+LLGV+GASKEAPGSN+RLLATIVAGSVLAGELSLMSA+AAG
Sbjct: 513 EVGTVGGGTQLASQSACLNLLGVKGASKEAPGSNARLLATIVAGSVLAGELSLMSAIAAG 572

Query: 108 QLVKSHMKYNRSTNDVSK 126
           QLV SHMKYNRS  DV+K
Sbjct: 573 QLVNSHMKYNRSNKDVTK 590

[Close]

BLAST of Lag0017113 vs. ExPASy Swiss-Prot
Match: Q00583 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 3 OS=Hevea brasiliensis OX=3981 GN=HMGR3 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.7e-29
Identity = 74/98 (75.51%), Postives = 83/98 (84.69%), Query Frame = 0


Query: 28  LTVVNFGWDGSRSTNSIVCFQVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLAT 87
           +  VN G D   S  S+   ++GTVGGGTQLASQSACL+LLGV+GASK++PGSNSRLLAT
Sbjct: 487 MEAVNDGKDLHISV-SMPSIELGTVGGGTQLASQSACLNLLGVKGASKDSPGSNSRLLAT 546

Query: 88  IVAGSVLAGELSLMSALAAGQLVKSHMKYNRSTNDVSK 126
           IVAGSVLAGELSLMSA+AAGQLV SHMKYNRS  DVSK
Sbjct: 547 IVAGSVLAGELSLMSAIAAGQLVNSHMKYNRSAKDVSK 583

[Close]

BLAST of Lag0017113 vs. ExPASy Swiss-Prot
Match: P29057 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 OS=Hevea brasiliensis OX=3981 GN=HMGR1 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.3e-29
Identity = 68/78 (87.18%), Postives = 76/78 (97.44%), Query Frame = 0


Query: 48  QVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALAAG 107
           +VGTVGGGTQLASQSACL+LLGV+GA+KE+PGSNSRLLA IVAGSVLAGELSLMSA+AAG
Sbjct: 495 EVGTVGGGTQLASQSACLNLLGVKGANKESPGSNSRLLAAIVAGSVLAGELSLMSAIAAG 554

Query: 108 QLVKSHMKYNRSTNDVSK 126
           QLVKSHMKYNRS+ D+SK
Sbjct: 555 QLVKSHMKYNRSSKDMSK 572

[Close]

BLAST of Lag0017113 vs. ExPASy Swiss-Prot
Match: U5JCC6 (3-hydroxy-3-methylglutaryl coenzyme A reductase 2-B OS=Panax ginseng OX=4054 GN=HMGR2 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.3e-29
Identity = 69/78 (88.46%), Postives = 76/78 (97.44%), Query Frame = 0


Query: 48  QVGTVGGGTQLASQSACLDLLGVRGASKEAPGSNSRLLATIVAGSVLAGELSLMSALAAG 107
           +VGTVGGGTQLASQSACL+LLGV+GASKE+PG NSRLLA+IVAGSVLAGELSLMSALAAG
Sbjct: 509 EVGTVGGGTQLASQSACLNLLGVKGASKESPGPNSRLLASIVAGSVLAGELSLMSALAAG 568

Query: 108 QLVKSHMKYNRSTNDVSK 126
           QLVKSHMK+NRS+ DVSK
Sbjct: 569 QLVKSHMKFNRSSKDVSK 586

The following BLAST results are available for this feature:

BLAST of Lag0017113 vs. NCBI nr


Match Name	E-value	Identity	Description
RVW78829.1	8.3e-29	90.00	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 [Vitis vinifera]	[more]
XP_027919759.1	4.1e-28	87.65	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Vigna unguiculata] >QCD8...	[more]
XP_010266284.1	4.1e-28	78.57	PREDICTED: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Nelumbo nucif...	[more]
XP_030539496.1	5.3e-28	91.03	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Rhodamnia argentea]	[more]
PIA56946.1	5.3e-28	78.57	hypothetical protein AQUCO_00700952v1 [Aquilegia coerulea]	[more]

BLAST of Lag0017113 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0A1C930	5.9e-30	88.46	3-hydroxy-3-methylglutaryl coenzyme A reductase 2-A OS=Panax ginseng OX=4054 GN=...	[more]
P48021	1.3e-29	88.46	3-hydroxy-3-methylglutaryl-coenzyme A reductase OS=Camptotheca acuminata OX=1692...	[more]
Q00583	1.7e-29	75.51	3-hydroxy-3-methylglutaryl-coenzyme A reductase 3 OS=Hevea brasiliensis OX=3981 ...	[more]
P29057	2.3e-29	87.18	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 OS=Hevea brasiliensis OX=3981 ...	[more]
U5JCC6	2.3e-29	88.46	3-hydroxy-3-methylglutaryl coenzyme A reductase 2-B OS=Panax ginseng OX=4054 GN=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023074	Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic domain superfamily	GENE3D	3.90.770.10		coord: 11..121 e-value: 7.7E-24 score: 86.5
IPR002202	Hydroxymethylglutaryl-CoA reductase, class I/II	PFAM	PF00368	HMG-CoA_red	coord: 45..118 e-value: 2.6E-23 score: 82.6
IPR002202	Hydroxymethylglutaryl-CoA reductase, class I/II	PANTHER	PTHR10572	3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE	coord: 29..125
IPR002202	Hydroxymethylglutaryl-CoA reductase, class I/II	PROSITE	PS50065	HMG_COA_REDUCTASE_4	coord: 43..118 score: 25.304047
None	No IPR available	PANTHER	PTHR10572:SF35	3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE	coord: 29..125
IPR023076	Hydroxymethylglutaryl-CoA reductase, class I/II, conserved site	PROSITE	PS01192	HMG_COA_REDUCTASE_3	coord: 103..116
IPR023076	Hydroxymethylglutaryl-CoA reductase, class I/II, conserved site	PROSITE	PS00318	HMG_COA_REDUCTASE_2	coord: 49..56
IPR009029	Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding domain superfamily	SUPERFAMILY	56542	Substrate-binding domain of HMG-CoA reductase	coord: 47..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017113.1	Lag0017113.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015936	coenzyme A metabolic process
biological_process	GO:0008299	isoprenoid biosynthetic process
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005778	peroxisomal membrane
molecular_function	GO:0004420	hydroxymethylglutaryl-CoA reductase (NADPH) activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor