Lag0016869 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016869
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionD-3-phosphoglycerate dehydrogenase
Locationchr12: 41975603 .. 41978716 (-)
RNA-Seq ExpressionLag0016869
SyntenyLag0016869
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTCCTCTGCAAATTCTCTCCTTTCCTCTGTCAACTTCTCCTCTTCCTTCAAATCCTCCATTTCCAATCCCAATTCCAAATCCCCTGTTCTTTCCTTTCCCACCTCCTCTGTTTCTCTCGACCTCCACTCTTTTTCTTCTTCTTCTTCCTCCCGCTGCAATTTGATTCCAGTCCGCAGCGTTTTGAAGACGGTGGAATCATCCGAGCTTCAGAGCTCCGATCTCGCCGACCCCACTTTCCCAGATGCAAGATCCACCATTTTAGTGGCGGAGAAGCTCGGCGAGGCCGGCCTCAAGCTTCTCCGGAGCTTCGGCGACGTCGAATGCGCTTACGACCTCTCGCCGGACGACCTCTGCTCCAAGATCTCTTCCTTCGACGCCTTGATCGTTCGGAGCGGCACCAAGGTCAGCCGGCAGGTCTTCGAGGCGGCCAAAGGACGTTTGAAGGTCGTCGGAAGAGCCGGCGTCGGCATCGACAATGTGGATCTGCAGGCCGCCACTGAGTTCGGTTGCCTCGTCGTTAATGCTCCGACTGCCAACACCATCGCCGCCGCTGAACATGGAATTGCGTTGCTTACTGCCATGGCTCGCAACGTCGCTCAGGCCGATGCCTCCATGAAAGCAGGTCGGTAATCGGTAATCGGTACTCTGTTTCGGAAACACGTAATTCATAAATACCCTTTGGTTTTAACGCTTAGATCTGCTTATAATTAGTAACCCAGATTCCAAATTCCAGGATTTCCCATGATTTTGAACTGTTTTGCTTGCTTTAAAGTTGAAAAATCATGATATATCCGAGATTTCCAAGCAGAATTATTCTGTAAAAGTGTCATCTTTTTGTGACCCCTGTTTTGGCTGGTAGTGTAGTCCCATTCAAAAAAGCACATGACAAGAATCAATTAAAATCTTGAATAAAAACCAAATGACCCACATCCCGATCTGGTAAAAAACTTAGGCTCTGTCTTAGTTCTGTTCTTCGTTGAAATGGGTTTCCAGGAAAATGGGAAAGGAGCAAGTACGTGGGAGTTTCACTGGTCGGAAAGACGCTGGCGGTGATGGGGTTCGGGAAAGTGGGATCGGAAGTGGCGAGACGAGCGAAGGGGTTGGGGATGCAGGTGATAGCGCACGATCCTTACGCGCCGGTGGACAGAGCGAGAGCCATCGGCGTCGAGCTGGTGTCGTTCAACCAAGCCATTTCCACCGCCGACTTCGTTTCGCTTCACATGCCGCTCACTCCGACGACGGCAAAGGTTTTCAACGACGATACTTTTGCTTTGATGAAGAAAGGCGCTCGCTTGATCAACGTTGCTAGAGGAGGTGTCATTGATGAAGATGCCCTTGTCAGAGCCCTCGACACTGGACTTGTTGCTCAGGTAATGATTAAATTTTCTAAAGTTTAGAAAGTTTAGAGACCGAATAGACATTTTTATTACCGATATGGTGAAATAGGCGGCCCTTGATGTGTTTGCGGAGGAGCCTCCGCCGACAGACAGCAAGCTGGTGCACCATAAGAATGTTACTGTCACGCCTCATCTTGGAGCCAGCACTAAGGAAGCACAGGCATGGATTTTACTTACTCATTTTATATATATTGAATTTTCTATTTCTTGGATTTAGGGAGGTAGGTAAAATTATTTTGAGTTTAGACAAATCAAAATAGATAATTGCTAAAATTATAGGTAAAGTAGCAAAAGTATTTTGAATCTATATCTAATAACTATTATAGGACATGCAAATTATTTTTTGTTTTTATTGAAATTACAAATTTTATATATTTTATAGGTTTAAATCGTATTTTGGTCCATATGCTTTGATTATTCTATTTTTGTCCTTACACTTTCAAAATGTTCGTTTTAGTCTAAGAACTTTCAAGTTTGTTCCATTTTGGTCCCTAAACTTTTAAAAGTTCTATTTTAGTCGTTGGACTTTCAAATGTAACTATTTTAGTCCTTGCTCTTATTTCAATTTTAACAAATTAATGACATAATATGTAAGTTAGAAATGAACATTAAACAAAGCAGATTTGAAGTATAAACATAACATAATAAGGACAAAAGAGATTAGGGATTAAAATAGAACAAATTTGAAAGTTTAAAGACCAAAATAAATGTTTTAAAAGTTTAAAGACTAAAGTAGAAGAAACTTAAAAGTCAACGAACTAAAATGAGATTCAAACCTACTTCGTACAAATCAACCATAGGTAGTAATTCTCTATTTTCTCGTTCCTAATCATATTTCAGGAAGGCGTTGCCATTGAAATAGCCGAAGCTGTCGTCGGAGCATTGAACGGTGAGCTTTCCGCCACCGCCGTCAACGCTCCCATGGTTGCCCCAGAGGTAATTCAAAATACTTATTTTTGTAATTTCCATCTACATTGATTTGAAGCCATAATCTTTTTTGAATTTCGAGATAAACGAACACTGTAATTATGAGCAGGTACTGTCGGAGCTGGCGCCGTACGTCGTACTGGCGGAAAAACTCGGCCGACTGGCGGTGCAGCTGGTGGCCGGCGGGAGCGGAATAAAATCAGTAAAAGTACTGTACAGATCCGGGCGAGCCCCAGACGATCTAGACACAAGGCTGTTACGAGCAATGATCACAAAAGGCATAATCGAGCCCATCTCCGACAGCCACATAAACCTCGTGAACGCCGATTTCACGGCGAAGCAAAAGGGGCTCCGAATAAGCGAGGAGCGCGTGCTGGTGGACGCGCCGTCGGAGTTCCCAGTGGATTCAATCCAAGTGTTCGTGTCCAACGTGGACTCGAAGTTCGCCAGCGCGGTGAACGAGGATGGCAACGTGGCAATCGAGGGGAGGGTGAAATACGGAGTGCCCCACCTGGTACGTGTCGGGTCGTTCGACGTGGACGTGAGTCTGGAAGGGAATTTAATCCTGTGCCGACAGCTGGACCAACCGGGGATGATCGGACGGGTGGGAAACATTCTGGGAGAGAACAACGTGAATGTGAATTTCATGAGCGTTGGAAGAACGTTGAGGAGAAAAAGGGCAATTATGGCAATTGGCGTCGATGAAGAACCCAATAAGGAAACTCTTAGGAAAATTGGACAAGTTTCTTCTGTTGAAGAGTTTGTGTTTTTGAACCTATAA

mRNA sequence

ATGGCTTCCTCCTCTGCAAATTCTCTCCTTTCCTCTGTCAACTTCTCCTCTTCCTTCAAATCCTCCATTTCCAATCCCAATTCCAAATCCCCTGTTCTTTCCTTTCCCACCTCCTCTGTTTCTCTCGACCTCCACTCTTTTTCTTCTTCTTCTTCCTCCCGCTGCAATTTGATTCCAGTCCGCAGCGTTTTGAAGACGGTGGAATCATCCGAGCTTCAGAGCTCCGATCTCGCCGACCCCACTTTCCCAGATGCAAGATCCACCATTTTAGTGGCGGAGAAGCTCGGCGAGGCCGGCCTCAAGCTTCTCCGGAGCTTCGGCGACGTCGAATGCGCTTACGACCTCTCGCCGGACGACCTCTGCTCCAAGATCTCTTCCTTCGACGCCTTGATCGTTCGGAGCGGCACCAAGGTCAGCCGGCAGGTCTTCGAGGCGGCCAAAGGACGTTTGAAGGTCGTCGGAAGAGCCGGCGTCGGCATCGACAATGTGGATCTGCAGGCCGCCACTGAGTTCGGTTGCCTCGTCGTTAATGCTCCGACTGCCAACACCATCGCCGCCGCTGAACATGGAATTGCGTTGCTTACTGCCATGGCTCGCAACGTCGCTCAGGCCGATGCCTCCATGAAAGCAGGAAAATGGGAAAGGAGCAAGTACGTGGGAGTTTCACTGGTCGGAAAGACGCTGGCGGTGATGGGGTTCGGGAAAGTGGGATCGGAAGTGGCGAGACGAGCGAAGGGGTTGGGGATGCAGGTGATAGCGCACGATCCTTACGCGCCGGTGGACAGAGCGAGAGCCATCGGCGTCGAGCTGGTGTCGTTCAACCAAGCCATTTCCACCGCCGACTTCGTTTCGCTTCACATGCCGCTCACTCCGACGACGGCAAAGGTTTTCAACGACGATACTTTTGCTTTGATGAAGAAAGGCGCTCGCTTGATCAACGTTGCTAGAGGAGGTGTCATTGATGAAGATGCCCTTGTCAGAGCCCTCGACACTGGACTTGTTGCTCAGGCGGCCCTTGATGTGTTTGCGGAGGAGCCTCCGCCGACAGACAGCAAGCTGGTGCACCATAAGAATGTTACTGTCACGCCTCATCTTGGAGCCAGCACTAAGGAAGCACAGGAAGGCGTTGCCATTGAAATAGCCGAAGCTGTCGTCGGAGCATTGAACGGTGAGCTTTCCGCCACCGCCGTCAACGCTCCCATGGTTGCCCCAGAGGTACTGTCGGAGCTGGCGCCGTACGTCGTACTGGCGGAAAAACTCGGCCGACTGGCGGTGCAGCTGGTGGCCGGCGGGAGCGGAATAAAATCAGTAAAAGTACTGTACAGATCCGGGCGAGCCCCAGACGATCTAGACACAAGGCTGTTACGAGCAATGATCACAAAAGGCATAATCGAGCCCATCTCCGACAGCCACATAAACCTCGTGAACGCCGATTTCACGGCGAAGCAAAAGGGGCTCCGAATAAGCGAGGAGCGCGTGCTGGTGGACGCGCCGTCGGAGTTCCCAGTGGATTCAATCCAAGTGTTCGTGTCCAACGTGGACTCGAAGTTCGCCAGCGCGGTGAACGAGGATGGCAACGTGGCAATCGAGGGGAGGGTGAAATACGGAGTGCCCCACCTGGTACGTGTCGGGTCGTTCGACGTGGACGTGAGTCTGGAAGGGAATTTAATCCTGTGCCGACAGCTGGACCAACCGGGGATGATCGGACGGGTGGGAAACATTCTGGGAGAGAACAACGTGAATGTGAATTTCATGAGCGTTGGAAGAACGTTGAGGAGAAAAAGGGCAATTATGGCAATTGGCGTCGATGAAGAACCCAATAAGGAAACTCTTAGGAAAATTGGACAAGTTTCTTCTGTTGAAGAGTTTGTGTTTTTGAACCTATAA

Coding sequence (CDS)

ATGGCTTCCTCCTCTGCAAATTCTCTCCTTTCCTCTGTCAACTTCTCCTCTTCCTTCAAATCCTCCATTTCCAATCCCAATTCCAAATCCCCTGTTCTTTCCTTTCCCACCTCCTCTGTTTCTCTCGACCTCCACTCTTTTTCTTCTTCTTCTTCCTCCCGCTGCAATTTGATTCCAGTCCGCAGCGTTTTGAAGACGGTGGAATCATCCGAGCTTCAGAGCTCCGATCTCGCCGACCCCACTTTCCCAGATGCAAGATCCACCATTTTAGTGGCGGAGAAGCTCGGCGAGGCCGGCCTCAAGCTTCTCCGGAGCTTCGGCGACGTCGAATGCGCTTACGACCTCTCGCCGGACGACCTCTGCTCCAAGATCTCTTCCTTCGACGCCTTGATCGTTCGGAGCGGCACCAAGGTCAGCCGGCAGGTCTTCGAGGCGGCCAAAGGACGTTTGAAGGTCGTCGGAAGAGCCGGCGTCGGCATCGACAATGTGGATCTGCAGGCCGCCACTGAGTTCGGTTGCCTCGTCGTTAATGCTCCGACTGCCAACACCATCGCCGCCGCTGAACATGGAATTGCGTTGCTTACTGCCATGGCTCGCAACGTCGCTCAGGCCGATGCCTCCATGAAAGCAGGAAAATGGGAAAGGAGCAAGTACGTGGGAGTTTCACTGGTCGGAAAGACGCTGGCGGTGATGGGGTTCGGGAAAGTGGGATCGGAAGTGGCGAGACGAGCGAAGGGGTTGGGGATGCAGGTGATAGCGCACGATCCTTACGCGCCGGTGGACAGAGCGAGAGCCATCGGCGTCGAGCTGGTGTCGTTCAACCAAGCCATTTCCACCGCCGACTTCGTTTCGCTTCACATGCCGCTCACTCCGACGACGGCAAAGGTTTTCAACGACGATACTTTTGCTTTGATGAAGAAAGGCGCTCGCTTGATCAACGTTGCTAGAGGAGGTGTCATTGATGAAGATGCCCTTGTCAGAGCCCTCGACACTGGACTTGTTGCTCAGGCGGCCCTTGATGTGTTTGCGGAGGAGCCTCCGCCGACAGACAGCAAGCTGGTGCACCATAAGAATGTTACTGTCACGCCTCATCTTGGAGCCAGCACTAAGGAAGCACAGGAAGGCGTTGCCATTGAAATAGCCGAAGCTGTCGTCGGAGCATTGAACGGTGAGCTTTCCGCCACCGCCGTCAACGCTCCCATGGTTGCCCCAGAGGTACTGTCGGAGCTGGCGCCGTACGTCGTACTGGCGGAAAAACTCGGCCGACTGGCGGTGCAGCTGGTGGCCGGCGGGAGCGGAATAAAATCAGTAAAAGTACTGTACAGATCCGGGCGAGCCCCAGACGATCTAGACACAAGGCTGTTACGAGCAATGATCACAAAAGGCATAATCGAGCCCATCTCCGACAGCCACATAAACCTCGTGAACGCCGATTTCACGGCGAAGCAAAAGGGGCTCCGAATAAGCGAGGAGCGCGTGCTGGTGGACGCGCCGTCGGAGTTCCCAGTGGATTCAATCCAAGTGTTCGTGTCCAACGTGGACTCGAAGTTCGCCAGCGCGGTGAACGAGGATGGCAACGTGGCAATCGAGGGGAGGGTGAAATACGGAGTGCCCCACCTGGTACGTGTCGGGTCGTTCGACGTGGACGTGAGTCTGGAAGGGAATTTAATCCTGTGCCGACAGCTGGACCAACCGGGGATGATCGGACGGGTGGGAAACATTCTGGGAGAGAACAACGTGAATGTGAATTTCATGAGCGTTGGAAGAACGTTGAGGAGAAAAAGGGCAATTATGGCAATTGGCGTCGATGAAGAACCCAATAAGGAAACTCTTAGGAAAATTGGACAAGTTTCTTCTGTTGAAGAGTTTGTGTTTTTGAACCTATAA

Protein sequence

MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLDLHSFSSSSSSRCNLIPVRSVLKTVESSELQSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL
Homology
BLAST of Lag0016869 vs. NCBI nr
Match: XP_038895985.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1043.5 bits (2697), Expect = 7.4e-301
Identity = 565/636 (88.84%), Postives = 595/636 (93.55%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLDLHSF------SSSSSSR 60
           MASSSA SL SS +F+S  +SS    +SKSP+LSF  SSVSL+LH        SSSSS+R
Sbjct: 1   MASSSAKSLFSSPHFTSFNRSS----HSKSPLLSFSNSSVSLNLHPLHSSSPSSSSSSNR 60

Query: 61  CNLIPVRSVLKTVESS--ELQSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECA 120
           CN IP+RSVLKTVESS  E+Q+SDL  PTFP+ RSTILVAEKLGEAGLKLLRSFG+VECA
Sbjct: 61  CNFIPLRSVLKTVESSGLEVQNSDLVGPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECA 120

Query: 121 YDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSP+DLC+KISS DALIVRSGTKV+RQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPT 300
           FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLTP 
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPA 300

Query: 301 TAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSK 360
           T+KVFNDDTFALMKKGARLINVARGGVIDEDALVRALD+G VAQAALDVF EEPPP DSK
Sbjct: 301 TSKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420
           LV HKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420

Query: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YVVLAEKLGRLAVQLVAGGSGIKSVKVLY+SGR PDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVLYKSGRVPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGR 540
           NLVNADFTAKQKGLRISEERVLVDAP EFPV+SIQ+ VS+VDSKFASAV E G+V IEG+
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQITVSHVDSKFASAVTESGDVVIEGK 540

Query: 541 VKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLR 600
           VKYGVPHL RVG FDVDVSLEGNLILCRQ+DQPGMIGRVG+ILGENNVNVNFMSVGRT R
Sbjct: 541 VKYGVPHLTRVGPFDVDVSLEGNLILCRQVDQPGMIGRVGSILGENNVNVNFMSVGRTTR 600

Query: 601 RKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           RKRAIMAIGVDEEPNK+TL+KIGQV +VEEFVFLNL
Sbjct: 601 RKRAIMAIGVDEEPNKDTLKKIGQVPAVEEFVFLNL 632

BLAST of Lag0016869 vs. NCBI nr
Match: KAG6606684.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036404.1 D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1030.4 bits (2663), Expect = 6.5e-297
Identity = 552/632 (87.34%), Postives = 588/632 (93.04%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD----LHSFSSSSSSRCN 60
           MASSSAN+L         F SS++    KSP+LS   SSVSL+    LHS SS+SSSRCN
Sbjct: 1   MASSSANAL---------FSSSLAPSTPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCN 60

Query: 61  LIPVRSVLKTVESSELQSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECAYDLS 120
            IP+RSVLKTVE+ EL +SDLADPTFP+ RSTILVAEKLGEAGLKLLRSFGDV CAYDLS
Sbjct: 61  FIPIRSVLKTVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVHCAYDLS 120

Query: 121 PDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           P+ LC+KISS DALIVRSGT V+RQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKV 240
           NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMGFGKV
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGFGKV 240

Query: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPTTAKV 300
           GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLTPTT+K+
Sbjct: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTSKI 300

Query: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSKLVHH 360
           FNDDTFALMKKGARLINVARGGVIDEDALVRALD+G+VAQAALDVFAEEPPP DSKLV H
Sbjct: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDSGVVAQAALDVFAEEPPPKDSKLVQH 360

Query: 361 KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420
           KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL
Sbjct: 361 KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420

Query: 421 AEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVN 480
           AEKLGRLAVQLVAGGSGIKS+KV+YR+GR PDDLDTRLLRAMITKGIIEPISDSHINLVN
Sbjct: 421 AEKLGRLAVQLVAGGSGIKSIKVVYRTGRTPDDLDTRLLRAMITKGIIEPISDSHINLVN 480

Query: 481 ADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGRVKYG 540
           ADFTAKQKGLRISEERV V+AP EFPVDSIQ+ VSNV+SKFASAV+E G VAIEG+VKYG
Sbjct: 481 ADFTAKQKGLRISEERVEVNAPPEFPVDSIQILVSNVESKFASAVSEKGEVAIEGKVKYG 540

Query: 541 VPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLRRKRA 600
           VPHL RV SF+VDVSLEG LILCRQ+DQPGMIGRVG+ILGENNVNVNFMSVGR  R+KRA
Sbjct: 541 VPHLTRVASFNVDVSLEGKLILCRQVDQPGMIGRVGSILGENNVNVNFMSVGRMTRKKRA 600

Query: 601 IMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           IMAIGVDEEPNKE+LRKIG+V +VEEFVFL+L
Sbjct: 601 IMAIGVDEEPNKESLRKIGEVPAVEEFVFLDL 623

BLAST of Lag0016869 vs. NCBI nr
Match: XP_022949286.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.5e-296
Identity = 554/632 (87.66%), Postives = 589/632 (93.20%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD----LHSFSSSSSSRCN 60
           MASSSAN+L     FSSS   SI     KSP+LS   SSVSL+    LHS SS+SSSRCN
Sbjct: 1   MASSSANAL-----FSSSLAPSI----PKSPLLSISNSSVSLNLLPTLHSSSSASSSRCN 60

Query: 61  LIPVRSVLKTVESSELQSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECAYDLS 120
            IP+RSVLKTVE+ EL +SDLADPTFP+ RSTILVAEKLGEAGLKLLRSFGDV+CAYDLS
Sbjct: 61  FIPIRSVLKTVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLS 120

Query: 121 PDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           P+ LC+KISS DALIVRSGT V+RQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKV 240
           NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMGFGKV
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGFGKV 240

Query: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPTTAKV 300
           GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLTPTT+K+
Sbjct: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTSKI 300

Query: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSKLVHH 360
           FNDDTFALMKKGARLINVARGGVIDEDALVRALD+G+VAQAALDVFAEEPPP DSKLV H
Sbjct: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDSGVVAQAALDVFAEEPPPKDSKLVQH 360

Query: 361 KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420
           KNVT TPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL
Sbjct: 361 KNVTFTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420

Query: 421 AEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVN 480
           AEKLGRLAVQLVAGGSGIKS+KV+YR+GR PDDLDTRLLRAMITKGIIEPISDSHINLVN
Sbjct: 421 AEKLGRLAVQLVAGGSGIKSIKVVYRTGRTPDDLDTRLLRAMITKGIIEPISDSHINLVN 480

Query: 481 ADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGRVKYG 540
           ADFTAKQKGLRISEERV V+AP EFPVDSIQ+ VSNV+SKFASAV+E G VAIEG+VKYG
Sbjct: 481 ADFTAKQKGLRISEERVEVNAPPEFPVDSIQILVSNVESKFASAVSEKGEVAIEGKVKYG 540

Query: 541 VPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLRRKRA 600
           VPHL RV SF+VDVSLEG LILCRQ+DQPGMIGRVG+ILGENNVNVNFMSVGR  R+KRA
Sbjct: 541 VPHLTRVASFNVDVSLEGKLILCRQVDQPGMIGRVGSILGENNVNVNFMSVGRMTRKKRA 600

Query: 601 IMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           IMAIGVDEEPNKE+LRKIG+V +VEEFVFL+L
Sbjct: 601 IMAIGVDEEPNKESLRKIGEVPAVEEFVFLDL 623

BLAST of Lag0016869 vs. NCBI nr
Match: XP_008463985.1 (PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1027.7 bits (2656), Expect = 4.2e-296
Identity = 560/636 (88.05%), Postives = 594/636 (93.40%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD---LHS---FSSSSSSR 60
           MASSS+ SL SS +F+S  +SS    +SKSP+LSF TSSVSL+   LHS    SSSSS+R
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSS----HSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSNR 60

Query: 61  CNLIPVRSVLKTVESSEL--QSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECA 120
           C+ IP+RSVLKT+ESS    Q+SDL DPT P+ RSTILVAEKLGEAGL+LLRSFG+V CA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSP++LC+KISS DALIVRSGTKV+RQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS+KAGKWER+KYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPT 300
           FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLT  
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTSA 300

Query: 301 TAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSK 360
           T+KVFNDDTF LMKKGARLINVARGGVIDEDALVRALDTG VAQAALDVF EEPPP DSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDTGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420
           LV HKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420

Query: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YVVLAEKLGRLAVQLVAGGSGIKSVKV+Y+SGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYKSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGR 540
           NLVNADFTAKQKGLRISEERVLVDAP EFPV+SIQV VS V+SKFASAV E G VAIEG+
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQVVVSEVESKFASAVTEKGEVAIEGK 540

Query: 541 VKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLR 600
           VKYGVPHL RVGSFDVDVSLEGNLILCRQ+DQPGMIGRVGNILGENNVNVNFMSVGRT+R
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTMR 600

Query: 601 RKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           RKRAIMAIGVDEEPNK+TLR+IGQV ++EEFVFLNL
Sbjct: 601 RKRAIMAIGVDEEPNKDTLRQIGQVPAIEEFVFLNL 632

BLAST of Lag0016869 vs. NCBI nr
Match: XP_023525881.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1026.2 bits (2652), Expect = 1.2e-295
Identity = 553/629 (87.92%), Postives = 588/629 (93.48%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD-LHSFSSSSSSRCNLIP 60
           MASSSAN+L     FSSS   SI     KSP+LS   SSVSL+ L +  SSSSSRCN IP
Sbjct: 1   MASSSANAL-----FSSSLAPSI----PKSPLLSISNSSVSLNLLPTLPSSSSSRCNFIP 60

Query: 61  VRSVLKTVESSELQSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECAYDLSPDD 120
           +RSVLKTVE+ EL +SDLADPTFP+ RSTILVAEKLGEAGLKLLRSFGDV+CAYDLSP+ 
Sbjct: 61  IRSVLKTVETLELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEH 120

Query: 121 LCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAP 180
           LC+KISS DALIVRSGT V+RQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAP
Sbjct: 121 LCAKISSCDALIVRSGTTVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAP 180

Query: 181 TANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSE 240
           TANTIAAAEHGIALLTAMARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMGFGKVGSE
Sbjct: 181 TANTIAAAEHGIALLTAMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGFGKVGSE 240

Query: 241 VARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPTTAKVFND 300
           VARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLTPTT+K+FND
Sbjct: 241 VARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTSKIFND 300

Query: 301 DTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSKLVHHKNV 360
           DTFALMKKGARLINVARGGVIDEDALVRALD+G+VAQAALDVFAEEPPP DSKLV HKNV
Sbjct: 301 DTFALMKKGARLINVARGGVIDEDALVRALDSGVVAQAALDVFAEEPPPKDSKLVQHKNV 360

Query: 361 TVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVLAEK 420
           TVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVLAEK
Sbjct: 361 TVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVLAEK 420

Query: 421 LGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADF 480
           LGRLAVQLVAGGSGIKS+KV+YR+GR PDDLDTRLLRAMITKGIIEPISDSHINLVNADF
Sbjct: 421 LGRLAVQLVAGGSGIKSIKVVYRTGRTPDDLDTRLLRAMITKGIIEPISDSHINLVNADF 480

Query: 481 TAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGRVKYGVPH 540
           TAKQKGLRISEERV V+AP E+PVDSIQ+ VSNV+SKFASAV+E G VAIEG+VKYGVPH
Sbjct: 481 TAKQKGLRISEERVEVNAPPEYPVDSIQILVSNVESKFASAVSEKGEVAIEGKVKYGVPH 540

Query: 541 LVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLRRKRAIMA 600
           L RV SF+VDVSLEG LILCRQ+DQPGMIGRVG+ILGENNVNVNFMSVGR  R+KRAIMA
Sbjct: 541 LTRVASFNVDVSLEGKLILCRQVDQPGMIGRVGSILGENNVNVNFMSVGRMTRKKRAIMA 600

Query: 601 IGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           IGVDEEPNKE+LRKIG+V +VEEFVFL+L
Sbjct: 601 IGVDEEPNKESLRKIGEVPAVEEFVFLDL 620

BLAST of Lag0016869 vs. ExPASy Swiss-Prot
Match: O04130 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH2 PE=1 SV=2)

HSP 1 Score: 865.9 bits (2236), Expect = 2.8e-250
Identity = 472/638 (73.98%), Postives = 537/638 (84.17%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLDLHSFSSSSSSRCNLIPV 60
           MA SS+ S + +VN  S + S   +P+S+  VL          LH   ++S     L  +
Sbjct: 1   MAFSSSCSSVKAVN--SRWTSPSPSPSSRFAVL-------PAFLHRRYATS---VKLTAI 60

Query: 61  RSVLKTVESSEL----------QSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVE 120
            + LKTVE + L            SD  +PT P  R  ILV EKLGEAG+ LLR FGDV+
Sbjct: 61  SAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVD 120

Query: 121 CAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATE 180
           C+YDLSP+DL  K++  DALIVRSGTKV+R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE
Sbjct: 121 CSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATE 180

Query: 181 FGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAV 240
            GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAGKWERSKYVGVSLVGKTLAV
Sbjct: 181 HGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGKWERSKYVGVSLVGKTLAV 240

Query: 241 MGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLT 300
           MGFGKVG+EVARRAKGLGM VI+HDPYAP DRARA+GV+LVSF+QAISTADFVSLHMPLT
Sbjct: 241 MGFGKVGTEVARRAKGLGMTVISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLT 300

Query: 301 PTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTD 360
           P T KVFND+TF+ MKKG RLINVARGGVIDEDALVRALD G+VAQAALDVF EEPP  D
Sbjct: 301 PATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKD 360

Query: 361 SKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSEL 420
           S+L+ H+NVTVTPHLGASTKEAQEGVAIEIAEAV GAL GELSATAVNAPMVAPEVLSEL
Sbjct: 361 SRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMVAPEVLSEL 420

Query: 421 APYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDS 480
            PY+VLAEKLGRLAVQL +GG G++S++V+YRS R  DDLDTRLLRAMITKGIIEPISDS
Sbjct: 421 TPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSARDRDDLDTRLLRAMITKGIIEPISDS 480

Query: 481 HINLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIE 540
           ++NLVNADF AKQKGLRISEER++VD+  E+PVDSIQV + NV+S FA AV++ G+++IE
Sbjct: 481 YVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDSIQVQILNVESNFAGAVSDAGDISIE 540

Query: 541 GRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRT 600
           G+VKYGVPHL  VGSF VDVSLEGNLILCRQ+DQPGMIG+VGNILGE NVNVNFMSVGRT
Sbjct: 541 GKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVNFMSVGRT 600

Query: 601 LRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           + RK+AIMAIGVDEEP+ +TL +IG VS++EEFVFL L
Sbjct: 601 VLRKQAIMAIGVDEEPDNKTLERIGGVSAIEEFVFLKL 624

BLAST of Lag0016869 vs. ExPASy Swiss-Prot
Match: O49485 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH1 PE=1 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 9.2e-238
Identity = 420/544 (77.21%), Postives = 493/544 (90.62%), Query Frame = 0

Query: 85  ARSTILVAEKLGEAGLKLLRSFGDVECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFE 144
           ++ TILVAEKLG+AG+KLL    +V+C+Y+++P++L  KIS  DALIVRSGTKV R+VFE
Sbjct: 60  SKPTILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFE 119

Query: 145 AAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQA 204
           ++ GRLKVVGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARNVAQA
Sbjct: 120 SSHGRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQA 179

Query: 205 DASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRAR 264
           DAS+KAG+W+R+KYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM+VIAHDPYAP DRA 
Sbjct: 180 DASVKAGEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAH 239

Query: 265 AIGVELVSFNQAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDA 324
           AIGV+LVSF++A++TADF+SLHMPLTPTT+K+ ND+TFA MKKG R++NVARGGVIDEDA
Sbjct: 240 AIGVDLVSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDA 299

Query: 325 LVRALDTGLVAQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAV 384
           LVRALD G+VAQAALDVF +EPP  DSKLV H+ VTVTPHLGAST EAQEGVAIEIAEAV
Sbjct: 300 LVRALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAV 359

Query: 385 VGALNGELSATAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSG 444
           VGALNGEL+ATAVNAPMV+ EVL+EL PYVVLAEKLGRLAVQLVAGGSG+K+ K+ Y S 
Sbjct: 360 VGALNGELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASA 419

Query: 445 RAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVD 504
           RA DDLDTRLLRAMITKGIIEPISD ++NLVNADFTAKQ+GLR+SEERVL+D   E P++
Sbjct: 420 RATDDLDTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLE 479

Query: 505 SIQVFVSNVDSKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQ 564
           +I V +SNV+SKFAS+++E G V +EG+VK GVPHL +VGSF+VDV+LEG++ILCRQ+DQ
Sbjct: 480 TITVQLSNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQ 539

Query: 565 PGMIGRVGNILGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFV 624
           PGMIG VG+ILGE+NVNVNFMSVGR   RK+AIMAIGVD+ P+KETL+KIG++ +VEEFV
Sbjct: 540 PGMIGTVGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFV 599

Query: 625 FLNL 629
           FL L
Sbjct: 600 FLKL 603

BLAST of Lag0016869 vs. ExPASy Swiss-Prot
Match: Q9LT69 (D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH3 PE=1 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 4.7e-226
Identity = 411/594 (69.19%), Postives = 495/594 (83.33%), Query Frame = 0

Query: 39  SVSLDLHSFSSSSS----SRCNLIPVRSVLKTVESSELQSSDLADPTFPDARSTILVAEK 98
           + SL+L S  SSSS    +  ++ P+R   + +  +   S     P       TILV EK
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRIILVTSSSSGGGGKP-------TILVTEK 61

Query: 99  LGEAGLKLLRSFGDVECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVG 158
           LG+AG+ LL+ + +V+C+YDLS ++LC+KIS  DALIVRSGTKV R VFE+++GRLKVVG
Sbjct: 62  LGQAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVG 121

Query: 159 RAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWE 218
           RAGVGIDNVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS+KAGKW 
Sbjct: 122 RAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWT 181

Query: 219 RSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFN 278
           R+KYVGVSLVGKTLAV+GFGKVGSEVARRA+GLGM VI HDPYAP DRARAIGVELVSF 
Sbjct: 182 RNKYVGVSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFE 241

Query: 279 QAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLV 338
            AISTADF+SLH+PLT  T+K+ ND TFA+MKKG R++NVARGGVIDE+AL+RALD+G+V
Sbjct: 242 VAISTADFISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIV 301

Query: 339 AQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSA 398
           AQAALDVF  EPP  D+KLV H++VT TPHLGAST EAQEGV+IE+AEAV+GAL GEL+A
Sbjct: 302 AQAALDVFTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAA 361

Query: 399 TAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRL 458
           TAVNAPMV  EVL EL PYVVLAEKLGRLAVQLV GGSG+ +VKV Y S RAPDDLDTRL
Sbjct: 362 TAVNAPMVPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRL 421

Query: 459 LRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVD 518
           LRAM+ KGIIEPIS   INLVN+D+ AKQ+G++ISEER+++D   E P++ I V ++NV+
Sbjct: 422 LRAMVIKGIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVE 481

Query: 519 SKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNI 578
           S+FASA++E G + +EGRVK GVP L +VG F VDVSLEG++ILCRQ+DQPGMIG+V +I
Sbjct: 482 SRFASALSESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASI 541

Query: 579 LGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           LG+ NVNV+FMSVGR    K+A+MAIGVDE+P+KETL+KIG + ++EEFVFL L
Sbjct: 542 LGDENVNVSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

BLAST of Lag0016869 vs. ExPASy Swiss-Prot
Match: Q58424 (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=serA PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.4e-105
Identity = 220/541 (40.67%), Postives = 328/541 (60.63%), Query Frame = 0

Query: 89  ILVAEKLGEAGLKLLRSFGDVECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKG 148
           ILV + L E  +K+L   G+VE A  L+ ++L  KI   D L+VRSGTKV+R V E A+ 
Sbjct: 4   ILVTDPLHEDAIKILEEVGEVEVATGLTKEELLEKIKDADVLVVRSGTKVTRDVIEKAE- 63

Query: 149 RLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASM 208
           +LKV+GRAGVG+DN+D++AATE G +VVNAP A++I+ AE  + L+ A ARN+ QA AS+
Sbjct: 64  KLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASL 123

Query: 209 KAGKWERSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGV 268
           K G+W+R ++ G+ L GKTL V+G G++G +V +RAK  GM +I +DPY P + A ++GV
Sbjct: 124 KRGEWDRKRFKGIELYGKTLGVIGLGRIGQQVVKRAKAFGMNIIGYDPYIPKEVAESMGV 183

Query: 269 ELV-SFNQAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVR 328
           ELV   N+    ADF++LH+PLTP T  +   +  ALMKK A ++N ARGG+IDE AL  
Sbjct: 184 ELVDDINELCKRADFITLHVPLTPKTRHIIGREQIALMKKNAIIVNCARGGLIDEKALYE 243

Query: 329 ALDTGLVAQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGA 388
           AL  G +  AALDVF EE PP D+ L+   NV  TPH GAST+EAQ+     +AE +   
Sbjct: 244 ALKEGKIRAAALDVF-EEEPPKDNPLLTLDNVIGTPHQGASTEEAQKAAGTIVAEQIKKV 303

Query: 389 LNGELSATAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAP 448
           L GEL+   VN P +  E L +L PY++LAE LG + +Q++ G   +  V+++Y    A 
Sbjct: 304 LRGELAENVVNMPNIPQEKLGKLKPYMLLAEMLGNIVMQVLDG--SVNRVELIYSGELAK 363

Query: 449 DDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQ 508
           +  D  L++    KG++ PI  + INLVNA   AK + + + E         E   ++I+
Sbjct: 364 EKTD--LIKRAFLKGLLSPILLAGINLVNAPIIAKNRNINVVES----STSEEKYGNAIK 423

Query: 509 VFVSNVDSKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGM 568
           +   +   KF          +I G +    P ++ V  ++V    EG L + + +D+PG 
Sbjct: 424 ITAESDKKKF----------SIVGAIINNKPVILEVDGYEVSFIPEGVLAIIKHIDRPGT 483

Query: 569 IGRVGNILGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLN 628
           IGRV   LG+  +N+  M VGR      ++M + +D    +E + KI ++ ++++   +N
Sbjct: 484 IGRVCITLGDYGINIASMQVGRKEPGGESVMLLNLDHTVPEEVIEKIKEIPNIKDVAVIN 524

BLAST of Lag0016869 vs. ExPASy Swiss-Prot
Match: O27051 (D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=serA PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.1e-100
Identity = 205/544 (37.68%), Postives = 320/544 (58.82%), Query Frame = 0

Query: 85  ARSTILVAEKLGEAGLKLLRSFGDVECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFE 144
           +R  +L+A+ + E G+  L    +V     ++P++L   I  FDA++VRS TKV+R+V E
Sbjct: 2   SRMKVLIADSINEKGISELEEVAEVVVNTTITPEELLDAIKDFDAIVVRSRTKVTREVIE 61

Query: 145 AAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQA 204
           AA  RLK++ RAGVG+DNVD++AAT+ G +V+NAP + +I  AEH I L+ A+AR +A A
Sbjct: 62  AAP-RLKIIARAGVGVDNVDVKAATDRGIMVINAPESTSITVAEHSIGLMLALARKIAIA 121

Query: 205 DASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRAR 264
           D S+K GKWE+++++G+ L GKTL ++G G++GS+V  R K  GM ++ +DPY   + A 
Sbjct: 122 DRSVKEGKWEKNRFMGIELNGKTLGIIGMGRIGSQVVVRTKAFGMDIMVYDPYISKEAAE 181

Query: 265 AIGVELVSFNQAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDA 324
            +GV +      +  +D V++H+PLTP T  + ++D F LMK  A ++N ARGG+IDEDA
Sbjct: 182 EMGVTVTDLETLLRESDIVTIHVPLTPETRHLISEDEFKLMKDTAFIVNCARGGIIDEDA 241

Query: 325 LVRALDTGLVAQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAV 384
           L RAL  G +A AALDVF EE PP  S L+  +NV +TPH+GAST EAQ   AI +A  +
Sbjct: 242 LYRALKDGEIAGAALDVF-EEEPPEGSPLLELENVVLTPHIGASTSEAQRDAAIIVANEI 301

Query: 385 VGALNGELSATAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSG 444
                G      +N P++  E    L PY+ LAEK+G +  Q + G   I+ + V Y   
Sbjct: 302 KTVFQGGAPRNVLNMPVMDSETYKSLKPYIELAEKMGAIIAQALPG--NIEKLDVTYCGE 361

Query: 445 RAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVD 504
            A    D  +L   + + I+ PI    +NL+NA   AK++G+ ++E R           D
Sbjct: 362 LAEMQFD--ILTRTMLQAILNPILTEPVNLINAPSIAKKRGIMVTEAR-------RSESD 421

Query: 505 SIQVFVSNVDSKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQ 564
             +  +       A+A ++ G  ++E       P ++ +  + VDV  EG +I+ R  D 
Sbjct: 422 GYRSII------IATAESDRGGFSVEA-THIKEPTIIGINGYRVDVKPEGTMIIARYRDL 481

Query: 565 PGMIGRVGNILGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFV 624
           PG IG +G  LG++ +N+  M VGR      A+M + VD+    E + ++ ++ +V++ V
Sbjct: 482 PGTIGAIGTKLGQHGINIATMQVGRKEIGGEAVMVLKVDQSVPAEVIEEVKKLDNVDDAV 525

Query: 625 FLNL 629
            + +
Sbjct: 542 AIEI 525

BLAST of Lag0016869 vs. ExPASy TrEMBL
Match: A0A6J1GBM1 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452687 PE=3 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 1.2e-296
Identity = 554/632 (87.66%), Postives = 589/632 (93.20%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD----LHSFSSSSSSRCN 60
           MASSSAN+L     FSSS   SI     KSP+LS   SSVSL+    LHS SS+SSSRCN
Sbjct: 1   MASSSANAL-----FSSSLAPSI----PKSPLLSISNSSVSLNLLPTLHSSSSASSSRCN 60

Query: 61  LIPVRSVLKTVESSELQSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECAYDLS 120
            IP+RSVLKTVE+ EL +SDLADPTFP+ RSTILVAEKLGEAGLKLLRSFGDV+CAYDLS
Sbjct: 61  FIPIRSVLKTVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLS 120

Query: 121 PDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           P+ LC+KISS DALIVRSGT V+RQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKV 240
           NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMGFGKV
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGFGKV 240

Query: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPTTAKV 300
           GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLTPTT+K+
Sbjct: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTSKI 300

Query: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSKLVHH 360
           FNDDTFALMKKGARLINVARGGVIDEDALVRALD+G+VAQAALDVFAEEPPP DSKLV H
Sbjct: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDSGVVAQAALDVFAEEPPPKDSKLVQH 360

Query: 361 KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420
           KNVT TPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL
Sbjct: 361 KNVTFTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420

Query: 421 AEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVN 480
           AEKLGRLAVQLVAGGSGIKS+KV+YR+GR PDDLDTRLLRAMITKGIIEPISDSHINLVN
Sbjct: 421 AEKLGRLAVQLVAGGSGIKSIKVVYRTGRTPDDLDTRLLRAMITKGIIEPISDSHINLVN 480

Query: 481 ADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGRVKYG 540
           ADFTAKQKGLRISEERV V+AP EFPVDSIQ+ VSNV+SKFASAV+E G VAIEG+VKYG
Sbjct: 481 ADFTAKQKGLRISEERVEVNAPPEFPVDSIQILVSNVESKFASAVSEKGEVAIEGKVKYG 540

Query: 541 VPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLRRKRA 600
           VPHL RV SF+VDVSLEG LILCRQ+DQPGMIGRVG+ILGENNVNVNFMSVGR  R+KRA
Sbjct: 541 VPHLTRVASFNVDVSLEGKLILCRQVDQPGMIGRVGSILGENNVNVNFMSVGRMTRKKRA 600

Query: 601 IMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           IMAIGVDEEPNKE+LRKIG+V +VEEFVFL+L
Sbjct: 601 IMAIGVDEEPNKESLRKIGEVPAVEEFVFLDL 623

BLAST of Lag0016869 vs. ExPASy TrEMBL
Match: A0A1S3CKG7 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 2.0e-296
Identity = 560/636 (88.05%), Postives = 594/636 (93.40%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD---LHS---FSSSSSSR 60
           MASSS+ SL SS +F+S  +SS    +SKSP+LSF TSSVSL+   LHS    SSSSS+R
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSS----HSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSNR 60

Query: 61  CNLIPVRSVLKTVESSEL--QSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECA 120
           C+ IP+RSVLKT+ESS    Q+SDL DPT P+ RSTILVAEKLGEAGL+LLRSFG+V CA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSP++LC+KISS DALIVRSGTKV+RQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS+KAGKWER+KYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPT 300
           FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLT  
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTSA 300

Query: 301 TAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSK 360
           T+KVFNDDTF LMKKGARLINVARGGVIDEDALVRALDTG VAQAALDVF EEPPP DSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDTGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420
           LV HKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420

Query: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YVVLAEKLGRLAVQLVAGGSGIKSVKV+Y+SGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYKSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGR 540
           NLVNADFTAKQKGLRISEERVLVDAP EFPV+SIQV VS V+SKFASAV E G VAIEG+
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQVVVSEVESKFASAVTEKGEVAIEGK 540

Query: 541 VKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLR 600
           VKYGVPHL RVGSFDVDVSLEGNLILCRQ+DQPGMIGRVGNILGENNVNVNFMSVGRT+R
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTMR 600

Query: 601 RKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           RKRAIMAIGVDEEPNK+TLR+IGQV ++EEFVFLNL
Sbjct: 601 RKRAIMAIGVDEEPNKDTLRQIGQVPAIEEFVFLNL 632

BLAST of Lag0016869 vs. ExPASy TrEMBL
Match: A0A0A0LC35 (D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.9e-296
Identity = 558/636 (87.74%), Postives = 593/636 (93.24%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD---LHSFSSSSSS---R 60
           MASSS+NSL SS +F+S  +S     +SK P+LSF TSSVSL+   LHS SSSSSS   R
Sbjct: 1   MASSSSNSLFSSPHFTSFNRSF----HSKPPLLSFSTSSVSLNPRPLHSSSSSSSSSSNR 60

Query: 61  CNLIPVRSVLKTVESSEL--QSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECA 120
           C+ IP+RSVLKT+ESS    Q+SDL DPT P+ RSTILVAEKLGEAGL+LLRSFG+V CA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSP++LC+KISS DALIVRSGTKV+RQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLT+MARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPT 300
           FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLTPT
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300

Query: 301 TAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSK 360
           T+KVFNDDTF LMKKGARLINVARGGVIDEDALVRALD+G VAQAALDVF EEPPP DSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420
           LV HKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMV PEVLSELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 420

Query: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YVVLAEKLGRLAVQLVAGGSGIKSVKV+YRSGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGR 540
           NLVNADFTAKQKGLRISEERVLVDAP EFPV+SIQ+ VS V+SKFASAV E G V IEG+
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 540

Query: 541 VKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLR 600
           VKYGVPHL RVGSFDVDVSLEGNLILCRQ+DQPGMIG+VGNILGENNVNVNFMSVGRTLR
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 600

Query: 601 RKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           RKRAIMAIG+DEEPNK+TL+KIGQV ++EEFVFLNL
Sbjct: 601 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 632

BLAST of Lag0016869 vs. ExPASy TrEMBL
Match: A0A5D3CA37 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G00710 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 1.0e-295
Identity = 559/640 (87.34%), Postives = 594/640 (92.81%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD---LH-------SFSSS 60
           MASSS+ S+ SS +F+S  +SS    +SKSP+LSF TSSVSL+   LH       S SSS
Sbjct: 1   MASSSSKSIFSSPHFTSFNRSS----HSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSSS 60

Query: 61  SSSRCNLIPVRSVLKTVESSEL--QSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGD 120
           SS+RC+ IP+RSVLKT+ESS    Q+SDL DPT P+ RSTILVAEKLGEAGL+LLRSFG+
Sbjct: 61  SSNRCSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGN 120

Query: 121 VECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAA 180
           V CAYDLSP++LC+KISS DALIVRSGTKV+RQVFEAAKGRLKVVGRAGVGIDNVDLQAA
Sbjct: 121 VVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAA 180

Query: 181 TEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTL 240
           TEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS+KAGKWER+KYVGVSLVGKTL
Sbjct: 181 TEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGKWERNKYVGVSLVGKTL 240

Query: 241 AVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMP 300
           AVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMP
Sbjct: 241 AVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMP 300

Query: 301 LTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPP 360
           LT  T+KVFNDDTF LMKKGARLINVARGGVIDEDALVRALDTG VAQAALDVF EEPPP
Sbjct: 301 LTSATSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDTGAVAQAALDVFVEEPPP 360

Query: 361 TDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLS 420
            DSKLV HKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLS
Sbjct: 361 KDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLS 420

Query: 421 ELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPIS 480
           ELAPYVVLAEKLGRLAVQLVAGGSGIKSVKV+Y+SGRAPDDLDTRLLRAMITKGIIEPIS
Sbjct: 421 ELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYKSGRAPDDLDTRLLRAMITKGIIEPIS 480

Query: 481 DSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVA 540
           DSHINLVNADFTAKQKGLRISEERVLVDAP EFPV+SIQV VS V+SKFASAV E G VA
Sbjct: 481 DSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQVVVSEVESKFASAVTEKGEVA 540

Query: 541 IEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVG 600
           IEG+VKYGVPHL RVGSFDVDVSLEGNLILCRQ+DQPGMIGRVGNILGENNVNVNFMSVG
Sbjct: 541 IEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVG 600

Query: 601 RTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           RT+RRKRAIMAIGVDEEPNK+TLR+IGQV ++EEFVFLNL
Sbjct: 601 RTMRRKRAIMAIGVDEEPNKDTLRQIGQVPAIEEFVFLNL 636

BLAST of Lag0016869 vs. ExPASy TrEMBL
Match: A0A6J1KGG7 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493010 PE=3 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.9e-295
Identity = 550/632 (87.03%), Postives = 588/632 (93.04%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLD----LHSFSSSSSSRCN 60
           MASSSAN+L     FSSS   SI     +SP+ S   SSVSL+    LHS S  SSSRCN
Sbjct: 1   MASSSANAL-----FSSSLAPSI----PESPLFSISNSSVSLNLLPTLHSSSFISSSRCN 60

Query: 61  LIPVRSVLKTVESSELQSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVECAYDLS 120
            IP+RSVLKTVE+ EL +SDLADPTFP+ RSTILVAEKLGEAGLKLLRSFGDV+CAYDLS
Sbjct: 61  FIPIRSVLKTVETLELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLS 120

Query: 121 PDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180
           P+ LC+KISS DALIVRSGT V+RQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV
Sbjct: 121 PEQLCAKISSCDALIVRSGTTVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVV 180

Query: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKV 240
           NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMGFGKV
Sbjct: 181 NAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGFGKV 240

Query: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLTPTTAKV 300
           GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADF+SLHMPLTPTT+K+
Sbjct: 241 GSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTSKI 300

Query: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTDSKLVHH 360
           FNDDTFALMKKGARLINVARGGVIDEDALVRALD+G+VAQAALDVFAEEPPP DSKLV H
Sbjct: 301 FNDDTFALMKKGARLINVARGGVIDEDALVRALDSGVVAQAALDVFAEEPPPKDSKLVQH 360

Query: 361 KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420
           KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL
Sbjct: 361 KNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAPYVVL 420

Query: 421 AEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVN 480
           AEKLGRLAVQLVAGGSGIKS++V+YR+GR PDDLDTRLLRAMITKGIIEPISD+HINLVN
Sbjct: 421 AEKLGRLAVQLVAGGSGIKSIRVVYRTGRTPDDLDTRLLRAMITKGIIEPISDNHINLVN 480

Query: 481 ADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIEGRVKYG 540
           ADFTAKQKGLRISEERV V+AP EFPVD+IQ+ +SNV+SKFASAV+E+G VAIEG+VKYG
Sbjct: 481 ADFTAKQKGLRISEERVEVNAPPEFPVDTIQILISNVESKFASAVSENGEVAIEGKVKYG 540

Query: 541 VPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRTLRRKRA 600
           VPHL RV SF+VDVSLEG LILCRQ+DQPGMIGRVG+ILGENNVNVNFMSVGR  R+KRA
Sbjct: 541 VPHLTRVASFNVDVSLEGKLILCRQVDQPGMIGRVGSILGENNVNVNFMSVGRMTRKKRA 600

Query: 601 IMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           IMAIGVDEEPNKE+LRKIG+V +VEEFVFLNL
Sbjct: 601 IMAIGVDEEPNKESLRKIGEVPAVEEFVFLNL 623

BLAST of Lag0016869 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 865.9 bits (2236), Expect = 2.0e-251
Identity = 472/638 (73.98%), Postives = 537/638 (84.17%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLDLHSFSSSSSSRCNLIPV 60
           MA SS+ S + +VN  S + S   +P+S+  VL          LH   ++S     L  +
Sbjct: 1   MAFSSSCSSVKAVN--SRWTSPSPSPSSRFAVL-------PAFLHRRYATS---VKLTAI 60

Query: 61  RSVLKTVESSEL----------QSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVE 120
            + LKTVE + L            SD  +PT P  R  ILV EKLGEAG+ LLR FGDV+
Sbjct: 61  SAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVD 120

Query: 121 CAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATE 180
           C+YDLSP+DL  K++  DALIVRSGTKV+R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE
Sbjct: 121 CSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATE 180

Query: 181 FGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAV 240
            GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAGKWERSKYVGVSLVGKTLAV
Sbjct: 181 HGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGKWERSKYVGVSLVGKTLAV 240

Query: 241 MGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFVSLHMPLT 300
           MGFGKVG+EVARRAKGLGM VI+HDPYAP DRARA+GV+LVSF+QAISTADFVSLHMPLT
Sbjct: 241 MGFGKVGTEVARRAKGLGMTVISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLT 300

Query: 301 PTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLVAQAALDVFAEEPPPTD 360
           P T KVFND+TF+ MKKG RLINVARGGVIDEDALVRALD G+VAQAALDVF EEPP  D
Sbjct: 301 PATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKD 360

Query: 361 SKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSEL 420
           S+L+ H+NVTVTPHLGASTKEAQEGVAIEIAEAV GAL GELSATAVNAPMVAPEVLSEL
Sbjct: 361 SRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMVAPEVLSEL 420

Query: 421 APYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRLLRAMITKGIIEPISDS 480
            PY+VLAEKLGRLAVQL +GG G++S++V+YRS R  DDLDTRLLRAMITKGIIEPISDS
Sbjct: 421 TPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSARDRDDLDTRLLRAMITKGIIEPISDS 480

Query: 481 HINLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVDSKFASAVNEDGNVAIE 540
           ++NLVNADF AKQKGLRISEER++VD+  E+PVDSIQV + NV+S FA AV++ G+++IE
Sbjct: 481 YVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDSIQVQILNVESNFAGAVSDAGDISIE 540

Query: 541 GRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNILGENNVNVNFMSVGRT 600
           G+VKYGVPHL  VGSF VDVSLEGNLILCRQ+DQPGMIG+VGNILGE NVNVNFMSVGRT
Sbjct: 541 GKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVNFMSVGRT 600

Query: 601 LRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           + RK+AIMAIGVDEEP+ +TL +IG VS++EEFVFL L
Sbjct: 601 VLRKQAIMAIGVDEEPDNKTLERIGGVSAIEEFVFLKL 624

BLAST of Lag0016869 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 844.3 bits (2180), Expect = 6.1e-245
Identity = 470/665 (70.68%), Postives = 537/665 (80.75%), Query Frame = 0

Query: 1   MASSSANSLLSSVNFSSSFKSSISNPNSKSPVLSFPTSSVSLDLHSFSSSSSSRCNLIPV 60
           MA SS+ S + +VN  S + S   +P+S+  VL          LH   ++S     L  +
Sbjct: 1   MAFSSSCSSVKAVN--SRWTSPSPSPSSRFAVL-------PAFLHRRYATS---VKLTAI 60

Query: 61  RSVLKTVESSEL----------QSSDLADPTFPDARSTILVAEKLGEAGLKLLRSFGDVE 120
            + LKTVE + L            SD  +PT P  R  ILV EKLGEAG+ LLR FGDV+
Sbjct: 61  SAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVD 120

Query: 121 CAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVGRAGVGIDNVDLQAATE 180
           C+YDLSP+DL  K++  DALIVRSGTKV+R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE
Sbjct: 121 CSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATE 180

Query: 181 FGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG-----------KW------ 240
            GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAG           +W      
Sbjct: 181 HGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGTLNYLFLVLLLRWNCRQSK 240

Query: 241 ----------ERSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRA 300
                     +RSKYVGVSLVGKTLAVMGFGKVG+EVARRAKGLGM VI+HDPYAP DRA
Sbjct: 241 HQYTIETETEKRSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRA 300

Query: 301 RAIGVELVSFNQAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDED 360
           RA+GV+LVSF+QAISTADFVSLHMPLTP T KVFND+TF+ MKKG RLINVARGGVIDED
Sbjct: 301 RALGVDLVSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDED 360

Query: 361 ALVRALDTGLVAQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEA 420
           ALVRALD G+VAQAALDVF EEPP  DS+L+ H+NVTVTPHLGASTKEAQEGVAIEIAEA
Sbjct: 361 ALVRALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEA 420

Query: 421 VVGALNGELSATAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRS 480
           V GAL GELSATAVNAPMVAPEVLSEL PY+VLAEKLGRLAVQL +GG G++S++V+YRS
Sbjct: 421 VAGALKGELSATAVNAPMVAPEVLSELTPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRS 480

Query: 481 GRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPV 540
            R  DDLDTRLLRAMITKGIIEPISDS++NLVNADF AKQKGLRISEER++VD+  E+PV
Sbjct: 481 ARDRDDLDTRLLRAMITKGIIEPISDSYVNLVNADFIAKQKGLRISEERMVVDSSPEYPV 540

Query: 541 DSIQVFVSNVDSKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLD 600
           DSIQV + NV+S FA AV++ G+++IEG+VKYGVPHL  VGSF VDVSLEGNLILCRQ+D
Sbjct: 541 DSIQVQILNVESNFAGAVSDAGDISIEGKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVD 600

Query: 601 QPGMIGRVGNILGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEF 629
           QPGMIG+VGNILGE NVNVNFMSVGRT+ RK+AIMAIGVDEEP+ +TL +IG VS++EEF
Sbjct: 601 QPGMIGQVGNILGEQNVNVNFMSVGRTVLRKQAIMAIGVDEEPDNKTLERIGGVSAIEEF 651

BLAST of Lag0016869 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 824.3 bits (2128), Expect = 6.5e-239
Identity = 420/544 (77.21%), Postives = 493/544 (90.62%), Query Frame = 0

Query: 85  ARSTILVAEKLGEAGLKLLRSFGDVECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFE 144
           ++ TILVAEKLG+AG+KLL    +V+C+Y+++P++L  KIS  DALIVRSGTKV R+VFE
Sbjct: 60  SKPTILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFE 119

Query: 145 AAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQA 204
           ++ GRLKVVGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARNVAQA
Sbjct: 120 SSHGRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQA 179

Query: 205 DASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRAR 264
           DAS+KAG+W+R+KYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM+VIAHDPYAP DRA 
Sbjct: 180 DASVKAGEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAH 239

Query: 265 AIGVELVSFNQAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDA 324
           AIGV+LVSF++A++TADF+SLHMPLTPTT+K+ ND+TFA MKKG R++NVARGGVIDEDA
Sbjct: 240 AIGVDLVSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDA 299

Query: 325 LVRALDTGLVAQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAV 384
           LVRALD G+VAQAALDVF +EPP  DSKLV H+ VTVTPHLGAST EAQEGVAIEIAEAV
Sbjct: 300 LVRALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAV 359

Query: 385 VGALNGELSATAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSG 444
           VGALNGEL+ATAVNAPMV+ EVL+EL PYVVLAEKLGRLAVQLVAGGSG+K+ K+ Y S 
Sbjct: 360 VGALNGELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASA 419

Query: 445 RAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVD 504
           RA DDLDTRLLRAMITKGIIEPISD ++NLVNADFTAKQ+GLR+SEERVL+D   E P++
Sbjct: 420 RATDDLDTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLE 479

Query: 505 SIQVFVSNVDSKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQ 564
           +I V +SNV+SKFAS+++E G V +EG+VK GVPHL +VGSF+VDV+LEG++ILCRQ+DQ
Sbjct: 480 TITVQLSNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQ 539

Query: 565 PGMIGRVGNILGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFV 624
           PGMIG VG+ILGE+NVNVNFMSVGR   RK+AIMAIGVD+ P+KETL+KIG++ +VEEFV
Sbjct: 540 PGMIGTVGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFV 599

Query: 625 FLNL 629
           FL L
Sbjct: 600 FLKL 603

BLAST of Lag0016869 vs. TAIR 10
Match: AT3G19480.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 785.4 bits (2027), Expect = 3.4e-227
Identity = 411/594 (69.19%), Postives = 495/594 (83.33%), Query Frame = 0

Query: 39  SVSLDLHSFSSSSS----SRCNLIPVRSVLKTVESSELQSSDLADPTFPDARSTILVAEK 98
           + SL+L S  SSSS    +  ++ P+R   + +  +   S     P       TILV EK
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRIILVTSSSSGGGGKP-------TILVTEK 61

Query: 99  LGEAGLKLLRSFGDVECAYDLSPDDLCSKISSFDALIVRSGTKVSRQVFEAAKGRLKVVG 158
           LG+AG+ LL+ + +V+C+YDLS ++LC+KIS  DALIVRSGTKV R VFE+++GRLKVVG
Sbjct: 62  LGQAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVG 121

Query: 159 RAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWE 218
           RAGVGIDNVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS+KAGKW 
Sbjct: 122 RAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWT 181

Query: 219 RSKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFN 278
           R+KYVGVSLVGKTLAV+GFGKVGSEVARRA+GLGM VI HDPYAP DRARAIGVELVSF 
Sbjct: 182 RNKYVGVSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFE 241

Query: 279 QAISTADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTGLV 338
            AISTADF+SLH+PLT  T+K+ ND TFA+MKKG R++NVARGGVIDE+AL+RALD+G+V
Sbjct: 242 VAISTADFISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIV 301

Query: 339 AQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSA 398
           AQAALDVF  EPP  D+KLV H++VT TPHLGAST EAQEGV+IE+AEAV+GAL GEL+A
Sbjct: 302 AQAALDVFTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAA 361

Query: 399 TAVNAPMVAPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVLYRSGRAPDDLDTRL 458
           TAVNAPMV  EVL EL PYVVLAEKLGRLAVQLV GGSG+ +VKV Y S RAPDDLDTRL
Sbjct: 362 TAVNAPMVPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRL 421

Query: 459 LRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPSEFPVDSIQVFVSNVD 518
           LRAM+ KGIIEPIS   INLVN+D+ AKQ+G++ISEER+++D   E P++ I V ++NV+
Sbjct: 422 LRAMVIKGIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVE 481

Query: 519 SKFASAVNEDGNVAIEGRVKYGVPHLVRVGSFDVDVSLEGNLILCRQLDQPGMIGRVGNI 578
           S+FASA++E G + +EGRVK GVP L +VG F VDVSLEG++ILCRQ+DQPGMIG+V +I
Sbjct: 482 SRFASALSESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASI 541

Query: 579 LGENNVNVNFMSVGRTLRRKRAIMAIGVDEEPNKETLRKIGQVSSVEEFVFLNL 629
           LG+ NVNV+FMSVGR    K+A+MAIGVDE+P+KETL+KIG + ++EEFVFL L
Sbjct: 542 LGDENVNVSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

BLAST of Lag0016869 vs. TAIR 10
Match: AT1G68010.1 (hydroxypyruvate reductase )

HSP 1 Score: 120.6 bits (301), Expect = 4.7e-27
Identity = 103/298 (34.56%), Postives = 147/298 (49.33%), Query Frame = 0

Query: 115 LSPDDLCSKI-SSFDALIVRSGTKVSRQVFEA-AKGRLKVVGRAGVGIDNVDLQAATEFG 174
           LS +D+   I    D +I +        +F A +K   K      VG +NVD++AA ++G
Sbjct: 50  LSVEDIIDLIGDKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYG 109

Query: 175 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG---KWERSKYVGVSLVGKTLA 234
             V N P   T   AE   +L  A AR + +AD  M+ G    W    +VG  L G+T+ 
Sbjct: 110 IAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVG 169

Query: 235 VMGFGKVGSEVAR-RAKGLGMQVIAHDPY----------APVDRARAIGVELVSFNQAIS 294
           V+G G++GS  AR   +G  M +I  D Y          A     +A G + V++ +A S
Sbjct: 170 VIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASS 229

Query: 295 ------TADFVSLHMPLTPTTAKVFNDDTFALMKKGARLINVARGGVIDEDALVRALDTG 354
                  AD +SLH  L  TT  + N +  A+MKK A L+N +RG VIDE ALV  L   
Sbjct: 230 MEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKEAILVNCSRGPVIDEAALVEHLKEN 289

Query: 355 LVAQAALDVFAEEPPPTDSKLVHHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNG 391
            + +  LDVF EEP      L   KN  V PH+ +++K  +EG+A   A  V+G + G
Sbjct: 290 PMFRVGLDVFEEEPFMKPG-LADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKG 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895985.17.4e-30188.84D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida][more]
KAG6606684.16.5e-29787.34D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosp... [more]
XP_022949286.12.5e-29687.66D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita moschata][more]
XP_008463985.14.2e-29688.05PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucumis mel... [more]
XP_023525881.11.2e-29587.92D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
O041302.8e-25073.98D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
O494859.2e-23877.21D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9LT694.7e-22669.19D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q584242.4e-10540.67D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC... [more]
O270511.1e-10037.68D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (st... [more]
Match NameE-valueIdentityDescription
A0A6J1GBM11.2e-29687.66D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452687... [more]
A0A1S3CKG72.0e-29688.05D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 ... [more]
A0A0A0LC355.9e-29687.74D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE... [more]
A0A5D3CA371.0e-29587.34D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1KGG72.9e-29587.03D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493010 P... [more]
Match NameE-valueIdentityDescription
AT1G17745.12.0e-25173.98D-3-phosphoglycerate dehydrogenase [more]
AT1G17745.26.1e-24570.68D-3-phosphoglycerate dehydrogenase [more]
AT4G34200.16.5e-23977.21D-3-phosphoglycerate dehydrogenase [more]
AT3G19480.13.4e-22769.19D-3-phosphoglycerate dehydrogenase [more]
AT1G68010.14.7e-2734.56hydroxypyruvate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015878S-adenosyl-L-homocysteine hydrolase, NAD binding domainSMARTSM00997AdoHcyase_NAD_2coord: 216..361
e-value: 0.0049
score: -40.0
NoneNo IPR availableGENE3D3.30.70.260coord: 555..628
e-value: 1.8E-20
score: 74.7
NoneNo IPR availableGENE3D3.40.50.720coord: 91..391
e-value: 8.0E-111
score: 371.5
NoneNo IPR availableGENE3D3.40.50.720coord: 182..364
e-value: 8.0E-111
score: 371.5
NoneNo IPR availablePANTHERPTHR42938:SF22D-3-PHOSPHOGLYCERATE DEHYDROGENASE 2, CHLOROPLASTICcoord: 53..623
NoneNo IPR availablePANTHERPTHR42938FORMATE DEHYDROGENASE 1coord: 53..623
NoneNo IPR availableCDDcd04902ACT_3PGDH-xctcoord: 556..628
e-value: 6.97196E-21
score: 84.8289
NoneNo IPR availableCDDcd12173PGDH_4coord: 89..391
e-value: 1.13501E-163
score: 468.434
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 546..623
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 88..219
IPR006236D-3-phosphoglycerate dehydrogenaseTIGRFAMTIGR01327TIGR01327coord: 89..626
e-value: 1.1E-165
score: 550.3
IPR002912ACT domainPFAMPF01842ACTcoord: 558..616
e-value: 3.9E-10
score: 39.3
IPR002912ACT domainPROSITEPS51671ACTcoord: 556..628
score: 10.240982
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 89..398
e-value: 1.4E-34
score: 119.4
IPR029009Allosteric substrate binding domain superfamilyGENE3D3.30.1330.90coord: 403..554
e-value: 4.2E-29
score: 103.2
IPR029009Allosteric substrate binding domain superfamilySUPERFAMILY143548Serine metabolism enzymes domaincoord: 404..550
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 191..366
e-value: 1.8E-62
score: 209.8
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00670D_2_HYDROXYACID_DH_2coord: 276..298
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00671D_2_HYDROXYACID_DH_3coord: 305..321
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 228..255
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 185..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016869.1Lag0016869.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006564 L-serine biosynthetic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0051287 NAD binding
molecular_function GO:0004617 phosphoglycerate dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor