Lag0016849 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016849
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein BIG GRAIN 1-like B
Locationchr12: 41834352 .. 41835458 (+)
RNA-Seq ExpressionLag0016849
SyntenyLag0016849
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGTGGGATCAGTCGTTTCAGAGAGACGATCGAGACCACCAGCACCGCCGTCGTTTCCGCCGCGACAATCCCTCTTTCTCTTCCTCCCTTCTCGACGCCATCTACCGTTCGATCGACGAATCCAACGGCCAGGGTGAAGACGGCCAGCTCATTTTTTACAGAGAAACCACCATGCGAACAAAACAGAGCATCGCCGCCGCCAGAACAGAGGACGACGACACCGTCAAGTTCCGGCGAGCTTGCATGATCGAGAAGTGGATGGACAAGAAGAAACTCAGAAACATTCGAGATTTTACAGTTTCCAGCTCGAGCTCGTCGGAATCCACCTCCACCGCCGGGAGAAGATTCTCCTCGTCGGAATCGGAGTTTCTGTCGCGAGCTCTGCACCGGCCGAAGCCGATCAAAACCGCCGCATGGACTCAAACGGAAATCCAAAAGCCAAAGCACGAAAATGGGTTCGTTAAAACCAAATCCAAAGCCTCGAAAATCTACCACGATTTGAAGAAAGTGAAACAGCCGATTTCTCCCGGCGGCCGCCTCGCTAATTTCCTCAACTCCCTCTTCAATGGCGGAAGCCCGAAAACGAAGCAAAAGATACCGAATCCTTCGATCAATTCAACGAGCTTCGACTACGACATGTCGACAAAATCCAAATCCCAACAGGGATCGACATGTTCCTCTGCTTCGTCGTTTTCGAGGTCTTGTCTGAGCAAAACGCCGTCTTCGAGAGGAAACATCAAAAGATCAGTTCGGTTTTGTCCTGTGAGTGTGATTGTCGACGAGGATTGTCAGCCATGTGGGCATAAAATTTTGCAGAAATTAGAGGAACCCATTGTGAAGAAAGGGTTGAAAAGTTATGGGAATTTTGAAACAGAGAAAGTGAAAACAGAGCATCAGATGAAGATTCATGGAAATGAAAGGTTTGATTATGAAGAAGAAGAAGAAGATGATGATGATGCAGTGAGTTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCGGTAATTGGAATTGAAAGGTACAGAGAGGAATTGCCAGTGTATGAAACTACGAATTTCAAAACAAATTGTGCCATTGCTAATGGATTGGTTTTGTAA

mRNA sequence

ATGGATTTGTGGGATCAGTCGTTTCAGAGAGACGATCGAGACCACCAGCACCGCCGTCGTTTCCGCCGCGACAATCCCTCTTTCTCTTCCTCCCTTCTCGACGCCATCTACCGTTCGATCGACGAATCCAACGGCCAGGGTGAAGACGGCCAGCTCATTTTTTACAGAGAAACCACCATGCGAACAAAACAGAGCATCGCCGCCGCCAGAACAGAGGACGACGACACCGTCAAGTTCCGGCGAGCTTGCATGATCGAGAAGTGGATGGACAAGAAGAAACTCAGAAACATTCGAGATTTTACAGTTTCCAGCTCGAGCTCGTCGGAATCCACCTCCACCGCCGGGAGAAGATTCTCCTCGTCGGAATCGGAGTTTCTGTCGCGAGCTCTGCACCGGCCGAAGCCGATCAAAACCGCCGCATGGACTCAAACGGAAATCCAAAAGCCAAAGCACGAAAATGGGTTCGTTAAAACCAAATCCAAAGCCTCGAAAATCTACCACGATTTGAAGAAAGTGAAACAGCCGATTTCTCCCGGCGGCCGCCTCGCTAATTTCCTCAACTCCCTCTTCAATGGCGGAAGCCCGAAAACGAAGCAAAAGATACCGAATCCTTCGATCAATTCAACGAGCTTCGACTACGACATGTCGACAAAATCCAAATCCCAACAGGGATCGACATGTTCCTCTGCTTCGTCGTTTTCGAGGTCTTGTCTGAGCAAAACGCCGTCTTCGAGAGGAAACATCAAAAGATCAGTTCGGTTTTGTCCTGTGAGTGTGATTGTCGACGAGGATTGTCAGCCATGTGGGCATAAAATTTTGCAGAAATTAGAGGAACCCATTGTGAAGAAAGGGTTGAAAAGTTATGGGAATTTTGAAACAGAGAAAGTGAAAACAGAGCATCAGATGAAGATTCATGGAAATGAAAGGTTTGATTATGAAGAAGAAGAAGAAGATGATGATGATGCAGTGAGTTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCGGTAATTGGAATTGAAAGGTACAGAGAGGAATTGCCAGTGTATGAAACTACGAATTTCAAAACAAATTGTGCCATTGCTAATGGATTGGTTTTGTAA

Coding sequence (CDS)

ATGGATTTGTGGGATCAGTCGTTTCAGAGAGACGATCGAGACCACCAGCACCGCCGTCGTTTCCGCCGCGACAATCCCTCTTTCTCTTCCTCCCTTCTCGACGCCATCTACCGTTCGATCGACGAATCCAACGGCCAGGGTGAAGACGGCCAGCTCATTTTTTACAGAGAAACCACCATGCGAACAAAACAGAGCATCGCCGCCGCCAGAACAGAGGACGACGACACCGTCAAGTTCCGGCGAGCTTGCATGATCGAGAAGTGGATGGACAAGAAGAAACTCAGAAACATTCGAGATTTTACAGTTTCCAGCTCGAGCTCGTCGGAATCCACCTCCACCGCCGGGAGAAGATTCTCCTCGTCGGAATCGGAGTTTCTGTCGCGAGCTCTGCACCGGCCGAAGCCGATCAAAACCGCCGCATGGACTCAAACGGAAATCCAAAAGCCAAAGCACGAAAATGGGTTCGTTAAAACCAAATCCAAAGCCTCGAAAATCTACCACGATTTGAAGAAAGTGAAACAGCCGATTTCTCCCGGCGGCCGCCTCGCTAATTTCCTCAACTCCCTCTTCAATGGCGGAAGCCCGAAAACGAAGCAAAAGATACCGAATCCTTCGATCAATTCAACGAGCTTCGACTACGACATGTCGACAAAATCCAAATCCCAACAGGGATCGACATGTTCCTCTGCTTCGTCGTTTTCGAGGTCTTGTCTGAGCAAAACGCCGTCTTCGAGAGGAAACATCAAAAGATCAGTTCGGTTTTGTCCTGTGAGTGTGATTGTCGACGAGGATTGTCAGCCATGTGGGCATAAAATTTTGCAGAAATTAGAGGAACCCATTGTGAAGAAAGGGTTGAAAAGTTATGGGAATTTTGAAACAGAGAAAGTGAAAACAGAGCATCAGATGAAGATTCATGGAAATGAAAGGTTTGATTATGAAGAAGAAGAAGAAGATGATGATGATGCAGTGAGTTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCGGTAATTGGAATTGAAAGGTACAGAGAGGAATTGCCAGTGTATGAAACTACGAATTTCAAAACAAATTGTGCCATTGCTAATGGATTGGTTTTGTAA

Protein sequence

MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSSSESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEHQMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL
Homology
BLAST of Lag0016849 vs. NCBI nr
Match: XP_022948938.1 (protein BIG GRAIN 1-like B [Cucurbita moschata])

HSP 1 Score: 594.0 bits (1530), Expect = 9.0e-166
Identity = 312/370 (84.32%), Postives = 332/370 (89.73%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD+SFQR D+D   RRR RRDNPSFSSSLLDAIYRSIDESN Q EDG L FYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSS 120
           RTKQS+  ARTEDD+ VKFRRACMIEKWMD+KK+RNIRDFTVSSSSSSESTSTAGRR+S+
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGG 180
           SESE LSRALHR KPIKT  WTQTE QK KHENG VKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSK 240
           RLANFLNSLFNGGSPKTKQK+PNPS N  S DYDM TK+KS QGSTCSSASSFSRSCLSK
Sbjct: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKS-QGSTCSSASSFSRSCLSK 240

Query: 241 TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEH 300
           TPSSRGN+KRSVRFCPVS IVDEDC+  GHK LQKLEEPI+KKGLKSYG FETE+VKTEH
Sbjct: 241 TPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETEEVKTEH 300

Query: 301 QMKIHGNERFDY--EEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKT 360
             K  GNE F+Y  E+EEE++DDAVSCSSSDLFELDNLSVIG+ERYREELPVYETTNFKT
Sbjct: 301 PRKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTNFKT 360

Query: 361 NCAIANGLVL 369
           NCAIA GLV+
Sbjct: 361 NCAIAKGLVV 369

BLAST of Lag0016849 vs. NCBI nr
Match: XP_022998766.1 (protein BIG GRAIN 1-like B [Cucurbita maxima])

HSP 1 Score: 592.0 bits (1525), Expect = 3.4e-165
Identity = 311/368 (84.51%), Postives = 330/368 (89.67%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD+SFQR D+D   RRR RRDNPSFSSSLLDAIYRSIDESN Q EDG L FYRETTM
Sbjct: 84  MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 143

Query: 61  RTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSS 120
           RTKQS+  ARTEDD+ VKFRRACMIEKWMD+KK+RNIRDFTVSSSSSSESTSTAGRR+S+
Sbjct: 144 RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 203

Query: 121 SESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGG 180
           SESE LSRALHR KPIKT  WTQTE QK KHENG VKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 204 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 263

Query: 181 RLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSK 240
           RLANFLNSLFNGGSPKTKQK+PNPS N TS DYDM TK+KS QGSTCSSASSFSRSCLSK
Sbjct: 264 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKS-QGSTCSSASSFSRSCLSK 323

Query: 241 TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEH 300
           TPSSRGN+KRSVRFCPVS IVDEDC+  GHK LQKLEEPI+KKGLKSYGNFETE+VKTEH
Sbjct: 324 TPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEH 383

Query: 301 QMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNC 360
             KI  NE F+Y    ED+DDAVSCSSSDLFELDNLSVIGIER+REELPVYETTNFKTNC
Sbjct: 384 PRKIKRNEGFEY----EDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNC 443

Query: 361 AIANGLVL 369
           AIA GL++
Sbjct: 444 AIAKGLLV 446

BLAST of Lag0016849 vs. NCBI nr
Match: XP_023525389.1 (protein BIG GRAIN 1-like B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589.7 bits (1519), Expect = 1.7e-164
Identity = 310/370 (83.78%), Postives = 330/370 (89.19%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD+SFQR D+D   RRR RRDNPSFSSSLLDAIYRSIDESN Q EDG L FYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSS 120
           RTKQS+  ARTEDD+ VKFRRACMIEKWMD+KK+RNIRDFTVSSSSSSESTSTAGRR+S+
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGG 180
           SESE LSRALHRPKPIKT  WTQTE QK KHENG VKTKSKASK+YHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRPKPIKTTPWTQTETQKAKHENGLVKTKSKASKMYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSK 240
           RLANFLNSLFNGGSPKTKQK PN S N  S DYDM TK+KS QGSTCSSASSFSRSCLSK
Sbjct: 181 RLANFLNSLFNGGSPKTKQKTPNRSTNPRSMDYDMPTKAKS-QGSTCSSASSFSRSCLSK 240

Query: 241 TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEH 300
           TP SRGN+KRSVRFCPVS IVDEDC+  GHK LQKLEEPI+KKGLKSYG FETE+VKTEH
Sbjct: 241 TPCSRGNVKRSVRFCPVSEIVDEDCEADGHKSLQKLEEPIMKKGLKSYGKFETEEVKTEH 300

Query: 301 QMKIHGNERFDY--EEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKT 360
             K  GNE FDY  E+EEE++DDAVSCSSSDLFELDNLSVIG+ERYREELPVYETTNFKT
Sbjct: 301 PRKSKGNEGFDYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTNFKT 360

Query: 361 NCAIANGLVL 369
           NCAIA GL++
Sbjct: 361 NCAIAKGLLV 369

BLAST of Lag0016849 vs. NCBI nr
Match: KAG6606698.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 588.2 bits (1515), Expect = 5.0e-164
Identity = 309/370 (83.51%), Postives = 331/370 (89.46%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD+SFQR D+D   RRR RRDNPSFSSSLLDAIYRSIDESN Q EDG L FYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSS 120
           RTKQS+  ARTEDD+ VKFRRACMIEKWMD+KK+RNIRDFTVSSSSSSESTSTAGRR+S+
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGG 180
           SESE LSRALHR KPIKT  WTQTE QK KHENG VKTKSKASK+YHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKVYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSK 240
           RLANFLNSLFNGGSPKTKQKIPNPS N  S DYDM+TK+KS QGSTCSSASSFSRSCLSK
Sbjct: 181 RLANFLNSLFNGGSPKTKQKIPNPSTNPRSMDYDMATKAKS-QGSTCSSASSFSRSCLSK 240

Query: 241 TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEH 300
           TPSSRGN+KRSVRFCPVS IV+EDC+  G K LQKLEEPI+KKGLKSYG FETE+VKTEH
Sbjct: 241 TPSSRGNVKRSVRFCPVSEIVEEDCEAYGQKSLQKLEEPIMKKGLKSYGKFETEEVKTEH 300

Query: 301 QMKIHGNERFDY--EEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKT 360
             K  GNE F+Y  E+EEE++DDA SCSSSDLFELDNLSVIG+ERYREELPVYETTNFKT
Sbjct: 301 PRKSKGNEGFEYEDEDEEEEEDDAGSCSSSDLFELDNLSVIGMERYREELPVYETTNFKT 360

Query: 361 NCAIANGLVL 369
           NCAIA GLV+
Sbjct: 361 NCAIAKGLVV 369

BLAST of Lag0016849 vs. NCBI nr
Match: KAG7036418.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.8 bits (1514), Expect = 6.5e-164
Identity = 311/371 (83.83%), Postives = 330/371 (88.95%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD+SFQR D+D   RRR RRDNPSFSSSLLDAIYRSIDESN Q EDG L FYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSS 120
           RTKQS+  ARTEDD+ VKFRRACMIEKWMD+KK+RNIRDFTVSSSSSSESTSTAGRR+S+
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGG 180
           SESE LSRALHR K IKT  WTQTE QK KHENG VKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKAIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSK 240
           RLANFLNSLFNGGSPKTKQKIPNPS N  S DYDM+TK+KS QGSTCSSASSFSRSCLSK
Sbjct: 181 RLANFLNSLFNGGSPKTKQKIPNPSTNPRSMDYDMATKAKS-QGSTCSSASSFSRSCLSK 240

Query: 241 TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEH 300
           TPSSRGN+KRSVRFCPVS IV+EDC+  G K LQKLEEPI+KKGLKSYG FETE+VKTEH
Sbjct: 241 TPSSRGNVKRSVRFCPVSEIVEEDCEAYGQKSLQKLEEPIMKKGLKSYGKFETEEVKTEH 300

Query: 301 QMKIHGNERFDYE---EEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFK 360
             K  GNE F+YE   EEEE+DDDA SCSSSDLFELDNLSVIG+ERYREELPVYETTNFK
Sbjct: 301 PRKSKGNEGFEYEDEDEEEEEDDDAGSCSSSDLFELDNLSVIGMERYREELPVYETTNFK 360

Query: 361 TNCAIANGLVL 369
           TNCAIA GLV+
Sbjct: 361 TNCAIAKGLVV 370

BLAST of Lag0016849 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.9e-57
Identity = 178/404 (44.06%), Postives = 218/404 (53.96%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRD--NPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRET 60
           MD WD        DHQHR R RRD  +PSFSS+LLD IYRSID+S+           R+T
Sbjct: 1   MDPWDT--YNIPLDHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSS-----MRKT 60

Query: 61  TMRTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRF 120
             + ++    +    DD   F R+         K L+ I       SSSS S S+    F
Sbjct: 61  KHQNREDTRVSANRRDD---FNRS---------KNLKTIEPVFFKHSSSSSSDSSG---F 120

Query: 121 SSSESEFLSR------ALHRPKPIKTAA---WTQTEIQKP----KHENG-FVKTKSKASK 180
           SSSES++  R      A+  PKPI+T         +  +P    K E+G F+KTKSKA K
Sbjct: 121 SSSESDYFYRRSKSSPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALK 180

Query: 181 IYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQG 240
           IY DLKKVKQPISPGGRLA FLNS+F G     K    N ++ ST+     +  + +   
Sbjct: 181 IYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLNKINTTVTSTT-----AAAAAASST 240

Query: 241 STCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKG 300
           +TCSSASSFSRSCLSKTPSS    KRSVRFCPV+VI DED     +            K 
Sbjct: 241 TTCSSASSFSRSCLSKTPSSSEKSKRSVRFCPVNVIFDEDSSKYNN------------KN 300

Query: 301 LKSYGNFETEKVKTEHQMK---IHGNERF---------DY-------------EEEEEDD 360
            K YGN E E     H ++   +  N R           Y             ++EE+DD
Sbjct: 301 NKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQKKNKEVIEVSVEDDEEDDD 360

Query: 361 DDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIA 364
           DDA+SC+SSDLFELDNLS IGI+RYREELPVYETT   TN  I+
Sbjct: 361 DDALSCTSSDLFELDNLSAIGIDRYREELPVYETTRLNTNRIIS 365

BLAST of Lag0016849 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.0e-55
Identity = 162/365 (44.38%), Postives = 205/365 (56.16%), Query Frame = 0

Query: 25  NPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVKFRRACM 84
           NPSFSS+LLD IYRSID+S+       +   +    +   S+   R  +   +  RR+  
Sbjct: 16  NPSFSSTLLDQIYRSIDDSSPPPPLESIKKKKHHHQQRNASLHEDR--EISPIYHRRSIA 75

Query: 85  IEKWMDKKKLRNIRDFTVSSS-----SSSESTSTAGRRFSSSESEFLSRALHRPKPIKTA 144
            +    ++K   +R    SSS     SSSES S  GR  SS+     SR   +PKPI+T+
Sbjct: 76  ADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRSKSSASPPSSSR--QQPKPIRTS 135

Query: 145 AWTQTE-IQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTK 204
           +   +  +QKPK   GF++TKSKA KIY DLKKVKQPISPGGRLA FLNSLF   +   K
Sbjct: 136 SVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAATNPK 195

Query: 205 QKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVS 264
           +     ++         +   +    STCSSASSFSRSCLSKTPSS G  KRSVRFCPV+
Sbjct: 196 KHKKTTTV---------AVVEEPHSSSTCSSASSFSRSCLSKTPSSSGKSKRSVRFCPVN 255

Query: 265 VIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEHQMKI----------HGNE 324
           VI+DED              P      + Y N E ++V+ EH+  I          H   
Sbjct: 256 VILDED---------SSFTMPYAYNNERLYDNNEAKRVE-EHRRVIQAAKDLLRTYHNKN 315

Query: 325 RFDYEE----EEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNF-KTNCAIA 369
           +         EE+D+DDA SC+SSDLFEL+NLS IGIERYREELPVYETT     N  IA
Sbjct: 316 KVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRVIA 357

BLAST of Lag0016849 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.1e-32
Identity = 131/368 (35.60%), Postives = 190/368 (51.63%), Query Frame = 0

Query: 22  RRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVKFRR 81
           R+  PSFSSS+LD++YRSIDES+G   D  L       + +  S  +   +DD     RR
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSD--LKGSINENVSSSSSSPSPNKKDDKLTTLRR 65

Query: 82  ACMIEK-WMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSSSESEFLSRALHRPKPIKTAA 141
           A M E+ W+  +        + ++++SS+S+S     FSSSE+E         K  +   
Sbjct: 66  AIMDEEHWLYARS-------STTTTNSSDSSS-----FSSSEAESYRTKRRLRKLAEQGK 125

Query: 142 WTQTEIQKPKH------ENGFVKT----KSKASKIYHDLKKVKQPISPGGRLANFLNSLF 201
            +  E Q+ K          F K+    K KA KI  +LK+ KQP+SPG RL +FLNS+F
Sbjct: 126 RSGDERQRTKRTVMDNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIF 185

Query: 202 NGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSKTPSSRGNIK- 261
              + K K                + +  K+    + SS S FSR+  +KT ++  N K 
Sbjct: 186 QSNAKKVK----------------LCSVGKTTDVKSSSSKSCFSRT-RNKTDNNNNNCKK 245

Query: 262 --RSVRFCPVSVIVDEDCQPCGHKILQKLEEPI----VKKGLK---SYGNFETEKVKTEH 321
             RS+RF PV V +D DC+    K + ++ +PI     KK +K      +  TE      
Sbjct: 246 LERSIRFYPVRVTIDGDCRDYAQKHITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITR 305

Query: 322 QMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNC 369
            + +    R +  E +E+++DA S SSSDLFELD+   IG+ RY +ELPVYETT+FKTN 
Sbjct: 306 NIGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYR-IGMGRYLKELPVYETTDFKTNQ 341

BLAST of Lag0016849 vs. ExPASy Swiss-Prot
Match: Q9LYH0 (Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 132/385 (34.29%), Postives = 185/385 (48.05%), Query Frame = 0

Query: 25  NPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVKFRRACM 84
           NPSFSS+LLD IY SID                 T +T+  + +  T        ++  +
Sbjct: 7   NPSFSSTLLDEIYNSIDPK---------------TQKTQPYVGSVNTTT------KKQSI 66

Query: 85  IEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSSSESEFL-------SRAL----HRP 144
           + + +  +K+   R F  S SSSS+S S+    FSSS++E         SR L     + 
Sbjct: 67  VTRSVPDRKIHRDR-FFGSVSSSSDSNSSI---FSSSDTELTHGKKTTSSRPLCFGPSKT 126

Query: 145 KPIKTAAWT----------------QTEIQK-PKHENGFVKTKSKAS-KIYHDLKKVKQP 204
           KP KT   T                 +++ K  +H+  +  T+++ S K   + KK K P
Sbjct: 127 KPRKTEDKTLFHQNRATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQKKPKTP 186

Query: 205 ISPGGRLANFLNSLFNGGS---------PKTKQKIPNPSINSTSFDYDMSTKSKSQQGST 264
            SPGGR+ NFLNSLF+  S         P+      +  +  TS DY  ST       +T
Sbjct: 187 ASPGGRIVNFLNSLFSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSST-------TT 246

Query: 265 CSSASSFSRSCLSK-TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQK-LEEPIVKKG 324
           CSSASSFSRSC++K    S G IKRSVRF PV+VIV E                  VKK 
Sbjct: 247 CSSASSFSRSCMNKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKSVKKN 306

Query: 325 LKSYGNFETEKVKTE--------HQ---MKIHGNERFDYEEEEEDDDDAVSCSSSDLFEL 355
           ++  G    E++  E        H+   +K +G E ++ ++E++DDDD  S SSSDLFEL
Sbjct: 307 VEDGGRRSVEEIAREFLRDYHKNHENSLVKTNGFEDYEDDDEDDDDDDVASDSSSDLFEL 356

BLAST of Lag0016849 vs. ExPASy Swiss-Prot
Match: B8APC7 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-24
Identity = 119/350 (34.00%), Postives = 169/350 (48.29%), Query Frame = 0

Query: 19  RRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVK 78
           RR+  D PSFSS+LLDAIY+S+DE  G G     +          +++AAA  +  +   
Sbjct: 15  RRYVADQPSFSSTLLDAIYKSMDEQPGHGGGATGV----------EAVAAAAKKQHEAAL 74

Query: 79  FRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSSSESEFLSRALHRPKPIKT 138
                         + R       ++SSSSE +S  G  FSSSE+E  S    R +PI+T
Sbjct: 75  HYGYYYKPSLAGSYRARAPGPH-ATTSSSSECSSYGG--FSSSEAE--SSHHRRLRPIRT 134

Query: 139 AAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTK 198
                     P+      K K   + I   L+ +++P SPG RLA FLNS+F G      
Sbjct: 135 TVPGGAPGPAPEK-----KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG------ 194

Query: 199 QKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVS 258
           ++ P    ++T           +   S CS+ASS+SRSCLSKTPS+RG  KR+VRF    
Sbjct: 195 KRAPATPPSAT-----------AGAESACSTASSYSRSCLSKTPSTRGQAKRTVRF---- 254

Query: 259 VIVDEDCQP-CGHKILQKLEEPIVKKGLKSYGNFETEKVKTEHQMKIHGNERFDYEEEEE 318
             +D D +      ++ +   P                V+   QM +   +R + E +E+
Sbjct: 255 --LDSDTESLASSTVVDRRRVP----------------VEAVQQMLL---QRMEMESDED 300

Query: 319 DDDDAVSCSSSDLFELDNLSVIGI--ERYREELPVYETTNFKTNCAIANG 366
           DD+   S +SSDLFEL+N + I      YR+ELPVYETT    N AI +G
Sbjct: 315 DDES--SDASSDLFELENFAAIAPAGAAYRDELPVYETTRVALNRAIGHG 300

BLAST of Lag0016849 vs. ExPASy TrEMBL
Match: A0A6J1GAP7 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.4e-166
Identity = 312/370 (84.32%), Postives = 332/370 (89.73%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD+SFQR D+D   RRR RRDNPSFSSSLLDAIYRSIDESN Q EDG L FYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSS 120
           RTKQS+  ARTEDD+ VKFRRACMIEKWMD+KK+RNIRDFTVSSSSSSESTSTAGRR+S+
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGG 180
           SESE LSRALHR KPIKT  WTQTE QK KHENG VKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSK 240
           RLANFLNSLFNGGSPKTKQK+PNPS N  S DYDM TK+KS QGSTCSSASSFSRSCLSK
Sbjct: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKS-QGSTCSSASSFSRSCLSK 240

Query: 241 TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEH 300
           TPSSRGN+KRSVRFCPVS IVDEDC+  GHK LQKLEEPI+KKGLKSYG FETE+VKTEH
Sbjct: 241 TPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETEEVKTEH 300

Query: 301 QMKIHGNERFDY--EEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKT 360
             K  GNE F+Y  E+EEE++DDAVSCSSSDLFELDNLSVIG+ERYREELPVYETTNFKT
Sbjct: 301 PRKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTNFKT 360

Query: 361 NCAIANGLVL 369
           NCAIA GLV+
Sbjct: 361 NCAIAKGLVV 369

BLAST of Lag0016849 vs. ExPASy TrEMBL
Match: A0A6J1KB46 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.7e-165
Identity = 311/368 (84.51%), Postives = 330/368 (89.67%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD+SFQR D+D   RRR RRDNPSFSSSLLDAIYRSIDESN Q EDG L FYRETTM
Sbjct: 84  MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 143

Query: 61  RTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSS 120
           RTKQS+  ARTEDD+ VKFRRACMIEKWMD+KK+RNIRDFTVSSSSSSESTSTAGRR+S+
Sbjct: 144 RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 203

Query: 121 SESEFLSRALHRPKPIKTAAWTQTEIQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGG 180
           SESE LSRALHR KPIKT  WTQTE QK KHENG VKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 204 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 263

Query: 181 RLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSK 240
           RLANFLNSLFNGGSPKTKQK+PNPS N TS DYDM TK+KS QGSTCSSASSFSRSCLSK
Sbjct: 264 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKS-QGSTCSSASSFSRSCLSK 323

Query: 241 TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEH 300
           TPSSRGN+KRSVRFCPVS IVDEDC+  GHK LQKLEEPI+KKGLKSYGNFETE+VKTEH
Sbjct: 324 TPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEH 383

Query: 301 QMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNC 360
             KI  NE F+Y    ED+DDAVSCSSSDLFELDNLSVIGIER+REELPVYETTNFKTNC
Sbjct: 384 PRKIKRNEGFEY----EDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNC 443

Query: 361 AIANGLVL 369
           AIA GL++
Sbjct: 444 AIAKGLLV 446

BLAST of Lag0016849 vs. ExPASy TrEMBL
Match: A0A1S3CKK1 (protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.1e-135
Identity = 289/385 (75.06%), Postives = 314/385 (81.56%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTM 60
           MDLWD S   D    Q  RR RRDNPSFSSSLLDAIYRSIDESN Q ED  LIFY   T 
Sbjct: 1   MDLWDHS---DPHLLQQPRRSRRDNPSFSSSLLDAIYRSIDESNSQPED-HLIFYTHNT- 60

Query: 61  RTKQSIAAAR-TEDDDTVKFRRACMIEKWMD-KKKLRNIRDFTVSSSSSSESTSTAGRRF 120
            TK +I  A  ++D +++KFR   MI+ W+D KKKLRNIRDFT+SSSSSSES+STAGRRF
Sbjct: 61  -TKHTILPATVSQDHESLKFR---MIDTWIDKKKKLRNIRDFTLSSSSSSESSSTAGRRF 120

Query: 121 SSSESEFLSRALHR-------PKPIKTAAWTQTEI--QKPKHENGFVKTKSKASKIYHDL 180
           SSSE+EFLSRALHR       PKPIKT  W QTEI    PKH+NGFVK+KSKASKIYHDL
Sbjct: 121 SSSETEFLSRALHRPTKLKPKPKPIKTNTWPQTEIITPNPKHDNGFVKSKSKASKIYHDL 180

Query: 181 KKVKQPISPGGRLANFLNSLFNGGSPKTKQKIPNP--SINSTSFDYDMSTKSKSQQG--S 240
           KKVKQPISPG RLA+FLNSLFNGGSPK KQKI +   SINST FDYDMS KSKSQQG  S
Sbjct: 181 KKVKQPISPGARLASFLNSLFNGGSPKAKQKISSSTCSINSTKFDYDMSRKSKSQQGSTS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKG- 300
           TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKIL K EEPI+KKG 
Sbjct: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKSEEPIMKKGN 300

Query: 301 -LKSYGNFETEKVKTEHQMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIER 360
             KS GN E EKVKTE  MKI+  E  + +++++DDDDA+SCSSSDLFELDNLSVIGIER
Sbjct: 301 LKKSNGNLEREKVKTEDMMKINYEEEEEEDDDDDDDDDALSCSSSDLFELDNLSVIGIER 360

Query: 361 YREELPVYETTNFKTNCAIANGLVL 369
           YREELPVYETT+FKTNCAIA GLVL
Sbjct: 361 YREELPVYETTHFKTNCAIAKGLVL 376

BLAST of Lag0016849 vs. ExPASy TrEMBL
Match: A0A6J1IZW5 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.8e-135
Identity = 278/384 (72.40%), Postives = 310/384 (80.73%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRD---HQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFY-R 60
           MDLWD SFQR+D D   H  RRR RR+NPSFSSSLLDAIYRSID+SN QGED QLIFY +
Sbjct: 1   MDLWDNSFQREDPDPHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNVQGED-QLIFYTQ 60

Query: 61  ETTMRTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGR 120
           +TT RTKQSI+AA+TED  T++F+RA MI+ WMD KK++ +RDFTVS+SSSSES+STAGR
Sbjct: 61  KTTTRTKQSISAAKTEDHQTLQFQRASMIKNWMDNKKIKTVRDFTVSTSSSSESSSTAGR 120

Query: 121 RFSSSESEFLSRALHR--------PKPIKTAAWTQTE-IQKPKHENGFVKTKSKASKIYH 180
           R SSSESEFLSR LHR        PKPIKT  W+QTE I  P HENGFVK+K +ASKIYH
Sbjct: 121 RLSSSESEFLSRTLHRPKPKPKPKPKPIKTTTWSQTEIIPNPNHENGFVKSKFRASKIYH 180

Query: 181 DLKKVKQPISPGGRLANFLNSLFNGGSPKTKQKIPNP-SINSTSFDYDMSTKSKSQQG-- 240
           DLKKVKQPISPGGRLANFLNSLFNGGSPK KQKI N  S NST+ DYD   KSKSQQG  
Sbjct: 181 DLKKVKQPISPGGRLANFLNSLFNGGSPKAKQKISNTCSNNSTNLDYDAPRKSKSQQGSS 240

Query: 241 STCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKG 300
           STCSSASSFSRSCL KTPSSRGNIKRSVRFCPVSVIVDEDC+PCGHK+L KLEEPI KK 
Sbjct: 241 STCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLHKLEEPIGKK- 300

Query: 301 LKSYGNFETEKVKTEHQMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERY 360
               G  ETE++KT               EE+++DDD +SCSSSDLFELDNLS IG+ERY
Sbjct: 301 ----GKLETEEMKT---------------EEDDEDDDRLSCSSSDLFELDNLSAIGMERY 360

Query: 361 REELPVYETTNFKTNCAIANGLVL 369
           R+ELPVYETT+F TNCAIANGL+L
Sbjct: 361 RDELPVYETTHFNTNCAIANGLLL 363

BLAST of Lag0016849 vs. ExPASy TrEMBL
Match: A0A6J1GWE3 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111457754 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.5e-134
Identity = 277/384 (72.14%), Postives = 309/384 (80.47%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRD-----HQHRRRFRRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFY 60
           MDLWD SFQR+D D     H  RRR RR+NPSFSSSLLDAIYRSID+SN QGED QLIFY
Sbjct: 1   MDLWDNSFQREDPDPHHHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNAQGED-QLIFY 60

Query: 61  -RETTMRTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTA 120
            ++TT RTKQSI+AA+TED  T++FRRA MIE WMD KK++ +RDFTVS+SSSSES+STA
Sbjct: 61  TQKTTTRTKQSISAAKTEDHQTLQFRRASMIENWMDNKKIKTLRDFTVSTSSSSESSSTA 120

Query: 121 GRRFSSSESEFLSRALHR------PKPIKTAAWTQTE-IQKPKHENGFVKTKSKASKIYH 180
           GRR SSSESEFLSR LHR      PKPIKT  W+QTE I  P HENGFVK+K KASKIYH
Sbjct: 121 GRRLSSSESEFLSRTLHRPKPKPKPKPIKTTTWSQTEIIPNPNHENGFVKSKFKASKIYH 180

Query: 181 DLKKVKQPISPGGRLANFLNSLFNGGSPKTKQKIPNP-SINSTSFDYDMSTKSKSQQG-- 240
           DLKKVKQPISPGGRLA+FLNSLFNGGSPK KQKI N  S NST+ +Y+   KSKSQQG  
Sbjct: 181 DLKKVKQPISPGGRLASFLNSLFNGGSPKAKQKISNTCSNNSTNLEYEAPRKSKSQQGSS 240

Query: 241 STCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKG 300
           STCSSASSFSRSCL KTPSSRGNIKRSVRFCPVSVIVDEDC+PCGHK+L KLEEPI KKG
Sbjct: 241 STCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLNKLEEPIGKKG 300

Query: 301 LKSYGNFETEKVKTEHQMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERY 360
                     K++TE             E++E+DDDD +SCSSSDLFELDNLS IG+ERY
Sbjct: 301 ----------KLETE-------------EDDEDDDDDRLSCSSSDLFELDNLSAIGMERY 360

Query: 361 REELPVYETTNFKTNCAIANGLVL 369
           R+ELPVYETT+F TNCAIANGL+L
Sbjct: 361 RDELPVYETTHFNTNCAIANGLLL 360

BLAST of Lag0016849 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 224.6 bits (571), Expect = 1.3e-58
Identity = 178/404 (44.06%), Postives = 218/404 (53.96%), Query Frame = 0

Query: 1   MDLWDQSFQRDDRDHQHRRRFRRD--NPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRET 60
           MD WD        DHQHR R RRD  +PSFSS+LLD IYRSID+S+           R+T
Sbjct: 1   MDPWDT--YNIPLDHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSS-----MRKT 60

Query: 61  TMRTKQSIAAARTEDDDTVKFRRACMIEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRF 120
             + ++    +    DD   F R+         K L+ I       SSSS S S+    F
Sbjct: 61  KHQNREDTRVSANRRDD---FNRS---------KNLKTIEPVFFKHSSSSSSDSSG---F 120

Query: 121 SSSESEFLSR------ALHRPKPIKTAA---WTQTEIQKP----KHENG-FVKTKSKASK 180
           SSSES++  R      A+  PKPI+T         +  +P    K E+G F+KTKSKA K
Sbjct: 121 SSSESDYFYRRSKSSPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALK 180

Query: 181 IYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQG 240
           IY DLKKVKQPISPGGRLA FLNS+F G     K    N ++ ST+     +  + +   
Sbjct: 181 IYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLNKINTTVTSTT-----AAAAAASST 240

Query: 241 STCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKG 300
           +TCSSASSFSRSCLSKTPSS    KRSVRFCPV+VI DED     +            K 
Sbjct: 241 TTCSSASSFSRSCLSKTPSSSEKSKRSVRFCPVNVIFDEDSSKYNN------------KN 300

Query: 301 LKSYGNFETEKVKTEHQMK---IHGNERF---------DY-------------EEEEEDD 360
            K YGN E E     H ++   +  N R           Y             ++EE+DD
Sbjct: 301 NKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQKKNKEVIEVSVEDDEEDDD 360

Query: 361 DDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIA 364
           DDA+SC+SSDLFELDNLS IGI+RYREELPVYETT   TN  I+
Sbjct: 361 DDALSCTSSDLFELDNLSAIGIDRYREELPVYETTRLNTNRIIS 365

BLAST of Lag0016849 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 217.2 bits (552), Expect = 2.1e-56
Identity = 162/365 (44.38%), Postives = 205/365 (56.16%), Query Frame = 0

Query: 25  NPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVKFRRACM 84
           NPSFSS+LLD IYRSID+S+       +   +    +   S+   R  +   +  RR+  
Sbjct: 16  NPSFSSTLLDQIYRSIDDSSPPPPLESIKKKKHHHQQRNASLHEDR--EISPIYHRRSIA 75

Query: 85  IEKWMDKKKLRNIRDFTVSSS-----SSSESTSTAGRRFSSSESEFLSRALHRPKPIKTA 144
            +    ++K   +R    SSS     SSSES S  GR  SS+     SR   +PKPI+T+
Sbjct: 76  ADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRSKSSASPPSSSR--QQPKPIRTS 135

Query: 145 AWTQTE-IQKPKHENGFVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTK 204
           +   +  +QKPK   GF++TKSKA KIY DLKKVKQPISPGGRLA FLNSLF   +   K
Sbjct: 136 SVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAATNPK 195

Query: 205 QKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVS 264
           +     ++         +   +    STCSSASSFSRSCLSKTPSS G  KRSVRFCPV+
Sbjct: 196 KHKKTTTV---------AVVEEPHSSSTCSSASSFSRSCLSKTPSSSGKSKRSVRFCPVN 255

Query: 265 VIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFETEKVKTEHQMKI----------HGNE 324
           VI+DED              P      + Y N E ++V+ EH+  I          H   
Sbjct: 256 VILDED---------SSFTMPYAYNNERLYDNNEAKRVE-EHRRVIQAAKDLLRTYHNKN 315

Query: 325 RFDYEE----EEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNF-KTNCAIA 369
           +         EE+D+DDA SC+SSDLFEL+NLS IGIERYREELPVYETT     N  IA
Sbjct: 316 KVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRVIA 357

BLAST of Lag0016849 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 141.4 bits (355), Expect = 1.5e-33
Identity = 131/368 (35.60%), Postives = 190/368 (51.63%), Query Frame = 0

Query: 22  RRDNPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVKFRR 81
           R+  PSFSSS+LD++YRSIDES+G   D  L       + +  S  +   +DD     RR
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSD--LKGSINENVSSSSSSPSPNKKDDKLTTLRR 65

Query: 82  ACMIEK-WMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSSSESEFLSRALHRPKPIKTAA 141
           A M E+ W+  +        + ++++SS+S+S     FSSSE+E         K  +   
Sbjct: 66  AIMDEEHWLYARS-------STTTTNSSDSSS-----FSSSEAESYRTKRRLRKLAEQGK 125

Query: 142 WTQTEIQKPKH------ENGFVKT----KSKASKIYHDLKKVKQPISPGGRLANFLNSLF 201
            +  E Q+ K          F K+    K KA KI  +LK+ KQP+SPG RL +FLNS+F
Sbjct: 126 RSGDERQRTKRTVMDNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIF 185

Query: 202 NGGSPKTKQKIPNPSINSTSFDYDMSTKSKSQQGSTCSSASSFSRSCLSKTPSSRGNIK- 261
              + K K                + +  K+    + SS S FSR+  +KT ++  N K 
Sbjct: 186 QSNAKKVK----------------LCSVGKTTDVKSSSSKSCFSRT-RNKTDNNNNNCKK 245

Query: 262 --RSVRFCPVSVIVDEDCQPCGHKILQKLEEPI----VKKGLK---SYGNFETEKVKTEH 321
             RS+RF PV V +D DC+    K + ++ +PI     KK +K      +  TE      
Sbjct: 246 LERSIRFYPVRVTIDGDCRDYAQKHITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITR 305

Query: 322 QMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNC 369
            + +    R +  E +E+++DA S SSSDLFELD+   IG+ RY +ELPVYETT+FKTN 
Sbjct: 306 NIGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYR-IGMGRYLKELPVYETTDFKTNQ 341

BLAST of Lag0016849 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 122.5 bits (306), Expect = 7.2e-28
Identity = 132/385 (34.29%), Postives = 185/385 (48.05%), Query Frame = 0

Query: 25  NPSFSSSLLDAIYRSIDESNGQGEDGQLIFYRETTMRTKQSIAAARTEDDDTVKFRRACM 84
           NPSFSS+LLD IY SID                 T +T+  + +  T        ++  +
Sbjct: 7   NPSFSSTLLDEIYNSIDPK---------------TQKTQPYVGSVNTTT------KKQSI 66

Query: 85  IEKWMDKKKLRNIRDFTVSSSSSSESTSTAGRRFSSSESEFL-------SRAL----HRP 144
           + + +  +K+   R F  S SSSS+S S+    FSSS++E         SR L     + 
Sbjct: 67  VTRSVPDRKIHRDR-FFGSVSSSSDSNSSI---FSSSDTELTHGKKTTSSRPLCFGPSKT 126

Query: 145 KPIKTAAWT----------------QTEIQK-PKHENGFVKTKSKAS-KIYHDLKKVKQP 204
           KP KT   T                 +++ K  +H+  +  T+++ S K   + KK K P
Sbjct: 127 KPRKTEDKTLFHQNRATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQKKPKTP 186

Query: 205 ISPGGRLANFLNSLFNGGS---------PKTKQKIPNPSINSTSFDYDMSTKSKSQQGST 264
            SPGGR+ NFLNSLF+  S         P+      +  +  TS DY  ST       +T
Sbjct: 187 ASPGGRIVNFLNSLFSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSST-------TT 246

Query: 265 CSSASSFSRSCLSK-TPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILQK-LEEPIVKKG 324
           CSSASSFSRSC++K    S G IKRSVRF PV+VIV E                  VKK 
Sbjct: 247 CSSASSFSRSCMNKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKSVKKN 306

Query: 325 LKSYGNFETEKVKTE--------HQ---MKIHGNERFDYEEEEEDDDDAVSCSSSDLFEL 355
           ++  G    E++  E        H+   +K +G E ++ ++E++DDDD  S SSSDLFEL
Sbjct: 307 VEDGGRRSVEEIAREFLRDYHKNHENSLVKTNGFEDYEDDDEDDDDDDVASDSSSDLFEL 356

BLAST of Lag0016849 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 50.8 bits (120), Expect = 2.7e-06
Identity = 73/242 (30.17%), Postives = 113/242 (46.69%), Query Frame = 0

Query: 146 IQKPKHENGFVKT-KSKASKIYHDLKKVKQPISPGGRLANFLNSLF-------NGGSPKT 205
           +Q+  HE   V T K +   + H     KQP SPGG++A+FLNSLF       N    K+
Sbjct: 91  LQQDHHEKHEVTTIKERLGNVRH-----KQPSSPGGKIASFLNSLFHQAGSKKNKSKSKS 150

Query: 206 KQKIPNPSINS-------------TSFDYDMSTKSKSQQGSTCSSASSFSRSCLSKTPSS 265
           K K  +P +               +S  +  S+ S+S   +T ++ASS S+S +S + S 
Sbjct: 151 KTKPTDPEVEEEIPGGGWMRRRRRSSISHFFSS-SRSTSTTTTTTASSSSKSLISSSSSG 210

Query: 266 -----------RGNIKRSVRFCPVSVIVDEDCQPCGHKILQKLEEPIVKKGLKSYGNFET 325
                        N K+ + +   +  V E+ +   +K    L+E +  K ++S    E 
Sbjct: 211 FRTPPPYLNTPTKNYKQFLNYTSATKQVGEE-ETKTNKEYSWLDEKL--KVMESLS--EN 270

Query: 326 EKVKTEHQMKIHGNERFDYEEEEEDDDDAVSCSSSDLFELDNLSVIGIERYREELPVYET 356
           +++ ++ +  I  + R    + E +DD   S SSSDLFEL N      E  R  LPVYET
Sbjct: 271 QRIWSDDE-DIDDDRRI---KREGEDDGMESDSSSDLFELQN-----YELSRGGLPVYET 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948938.19.0e-16684.32protein BIG GRAIN 1-like B [Cucurbita moschata][more]
XP_022998766.13.4e-16584.51protein BIG GRAIN 1-like B [Cucurbita maxima][more]
XP_023525389.11.7e-16483.78protein BIG GRAIN 1-like B [Cucurbita pepo subsp. pepo][more]
KAG6606698.15.0e-16483.51Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7036418.16.5e-16483.83Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9SLL21.9e-5744.06Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=... [more]
Q9LVK23.0e-5544.38Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=... [more]
Q9M2B32.1e-3235.60Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=... [more]
Q9LYH01.0e-2634.29Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=... [more]
B8APC71.2e-2434.00Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GAP74.4e-16684.32protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV... [more]
A0A6J1KB461.7e-16584.51protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1[more]
A0A1S3CKK11.1e-13575.06protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1[more]
A0A6J1IZW51.8e-13572.40protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1[more]
A0A6J1GWE31.5e-13472.14protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111457754 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G54200.11.3e-5844.06unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G13980.12.1e-5644.38unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G42800.11.5e-3335.60unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12050.17.2e-2834.29unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G69160.12.7e-0630.17unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 193..246
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..126
NoneNo IPR availablePANTHERPTHR33541:SF2PROTEIN BIG GRAIN 1-LIKE Acoord: 14..368
IPR039621Protein BIG GRAIN 1-likePANTHERPTHR33541PROTEIN BIG GRAIN 1-LIKE A-RELATEDcoord: 14..368

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016849.1Lag0016849.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0005886 plasma membrane