Lag0016804 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016804
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGlucose-methanol-choline (GMC) oxidoreductase family protein
Locationchr12: 41424537 .. 41430963 (+)
RNA-Seq ExpressionLag0016804
SyntenyLag0016804
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTAGGGTCGAAATCGTATTCGGAATTCCGATACCCGTTTATCAAACGGGCGAGCTCGTTCTCGTCGTTCTCGTCGTCGACGTATTCGTCGCGTCGGAGCGAGAATGTTTACGACTATATAATTGTGGGGGAGGCACGGCGGGGTGTCCCTTGGCAACGACATTGTCGAAAAGATTTAGCGTTTTATTGCTCGAGAGAGGAGGAGTCCCTTTTGCCAATTCAAATGTGTCATTTTTGAAGAACTTTCATATTACTTTGGCTGACATTTCACCCACTTCAGCTTCCCAAGCCTTCATTTCTACTGATGGAGTTCTCAATGCTAGGGCTAGGGTTTTGGGTGGTGGCACTTCCATCAATGCTGGTTTCTACACTAGAGCAAGCTCAAGGTACATACATTTTTTTTTAATAAACAATCTCTTGATTTATTCGACCCCATTTTGAAAACGTTCCGAAGAGTTTCAAGATTAAATCACGACAATGCTAAGTTTTTTCTTTTAATATGTGGGGTTGGGAGATTATTCGTACGTAAACAATACCTGGTTGCATATCAATGTATAGGAATCTCTCTACACACTGCGATTGAAATGTTGACAACTAGGAGAAAAGAAACAAATTTCCTATTCTTCTCTCTCTTAGTTGTTAACATCTCAATGCTGATATGTAGAGATCCTTACCGTACTCCGTTATGCAACCAATTAGTATGATTTTCATTCCTTTGACCTCTTGATTGATGATATATGTTTAAACTATTTGAGTTATGTCCAGGTTGGTGTCGTACAGTGCTAAGTTTGTGATAGTATTATTGTGGTTGGTGTTCGAGTTTTATTATTTATTCATATCATTAATGGTTGATAAAATAATTAAGCTTTGTATCAAATTTAAGTTCCTTTTGCATTTTTTTATACATTGTGCTTGAGAGAAATTTGAAAGGGAAAGAAAATGGTTAAAAGATGCATTTATTGTAAACAAATCATCTCAAAATATACTAGTGTGTGAAGTGATGAACTTTAATGCACTTAAGAGGCCTAGCTGGCTAGCTTCTTAAATAGGTGTGCCCTATCACACAATTAGTAGATTTTGTTTGGTTTAAATTTAAATATTGGTTTGGTCTCTAAACTTTAAATTTAAATTTTTTATATATTATTTAATATTGACCTTGTATGGCTGTTTAGTTATTATTGTTGAATAATTATGGTATTATTGTTAAAATTATAATAAATAAAGTGGACTCATTTAGTTAGATATATTTACTAAATTTGTATATAAATATGTATACAAAAAAGAATACTTGTTTTCTTGGAAGAGTTTTTTTTTTCTTTTGATAGAGTTGTTTTTTTTTTCTTTCTTTTCTATCTATAGAGACAGGGTGAGTTAAAGATTTGAAATAGTTTAATTAATTAGTTAATACACACATATACACGTGCGATGAAACTACTATATGACCTATTTATAATTAGCTATGCAGCTTTTATCAATGAAGTGAGGTCTCAATCTTATCTGTTCATTATTGTGGAATGAGAATTTCACTTAAAGAAAAAAATTAATTTTTTGGTCTCATGTGTTTTAATCAAAACAACATCTTTATATTCCATCAAAGTGCATATTTCTTAACTTACTGAACTCTCACATGATTAAAATAAAACGTTATATTATTGTGAAACTCATCAGATGCATTCATAAATAGTTTACCTAGTTAGATTATTATTGCTATTATTTTACATCATTGGTCGAGATGTTTACTAAACTTTCATTATCGGATTAAACTAAAGCTAAAACATAGGGACACGATTTAAGGATATAGAAAATTTGAACACAACAACAGTTTAGTTTATTGAAAATTTTCACACAAATAATAGTGACCACAAAGTCACACATTAATTACAATGACATATATAGTACCATAAATTCAGAACATTTATTATTTCCATCTTCCCCTTCTTAATGTTTTAATTAAATGAGAGTCTCTCTCTCCAATTTAATATTTTCTCCCTTTGTAGGTCATAAAAGTGTGTTAGGTGGAGTTTGTGTGTATTGCAAACTTAGAATTTATTGAAAATAATTTAAATGTTTTGACTACTTTCTAAAAGAAAAAAAATATTTTGACTTATATCCTTTTTCTTTTTTTTTTTTCCTTTTTAAATTTTTTTAAAAATATTAGTCTATCCCTTTTTAGATGGAAGGATCGAAGCTCTTTCCTTATTTAGTCAAGGTGAACATAGTTTAACAATAATGACATTTACTCATTCCCTTGAAGTTAGAGGTTCAGTTTGTTCAAATTTTTATACCTACATTTTTTGTGCTTAAAAAAAATATATATTTTCCATTATTTATTATTTAATAATTTTTTTAAAAAATGTTCCATAATTTTATTTGGGGTTACACGAAGGGTTACAATGTCTTTTTAGTCTATGGGCCCGAAAGCCCAATTCAGATGTTTAAAGTCCCATTTGGAGCCCATTTCAATCTATACCTAATACAAAAAGTTTTAAATTTTAAATTTTATTTTCTTAAACTTAGATGCTTATTTATTTTAAATCAAAGCCAACTTAAACCAAACATAATAATTGGTTAAGGTACTATATCTTCGTATCGCTCTATTAGAAAAAAAAAAGTTTAATCCTCCCTTTCGAATCGGGAAGTTTCAATAAAGCTTATGATTTTGAAATACGAGAAATGATTTTAAATTCTTTGTCTCATTTGGAACAAGAAAGATTTAAAATGAATGTATTTCTATTTGTGATAATAATATATTTGAACTTTGTAACTAAGGATGGCAACGGGGCGGGACGGGACCGAGGATGCACTTCCCATCCTTGTCTCCGTGGGAGTTTTCATCCCCATCCATTCTCTGTTTTGGAGAATCGGGGTCGGGGATCTCCATTTGGGGATCAAGTCCCTGCGGGAAAAAAAATTCCCGCTTTTTTTTTTTTTTTTAATAAAGTAATAAAAATTATTTAAAATATACCTAATTTGTTTAAGATATTAAAAAAATTTATTATAAATTTAAACACCAAAATTAATTTTTTTAAAAAAAAAAAACAAAAACTAAATAAAACAATATTGTTAATTTTTTTTAGAAAACAGTAATGTTAACTTTTTTTTTTTTTTTTGAAAAGAACAGTATTGTTAACTTAATAATTGAAAAAAAACCTTTATAAAATATTAATAATAATATAATATGATATATTAATAATATTATTATATAATTATGATATATATATATATTTTAAAAATCAATAAAAAATAATATACAGGACGGGTTCGGGGTCGGGGACCCGAAAAGAATCCCCAACTTGACCCTTATACTCAGTTGGGGATTCCTCGCCCCATTTGGCACAGGGACCCGAAGGAATTTTTGTCATCCCTATTTGTAACCATACATTGCAATTATACATACTCAATATCTGACTTTTTAGTTAAGAAATACACCAACTTTTATCTAATCACACTTTTTTTCCTTTTTTTTTTTTTTTCTTTTTTGAGAAACTAACTTTTACCTAATCACTTTCCAATCTTTATTTTCATGAGAATATTTTCATTAACAATTTTTCTAATTAAAATCAATCTACATAATTAAGATTGTAACACTAACTTAAATTTAAGCTATTTCAAAAAAGAAAAAAAAGTTCAAATTTGTATCCTCAAAATTTAAACCCAAAATTGGAAGTCTAAGTGTAAACATCTTTCCAAGCTTGAAGAAAAAAAAAAAAAGATCAGCGAAGAAATGGCAGAGCTACAGCTAACAATGGTGAATCAACCGATGGTTTCAGGTTCATAGAGAAAGTGGGTTGGGACGCGAAGCTTGTGAATGAATCATACCCATGGATCGAGGCTCAGATTGTTCACCGCCCCAAGCTCACGCCATGGCAGAGAGCTTTCAGAGACAGTCTTTTGGACGTCGGTGTCTCCCCCTTCAATGGCTTCACCTACGATCATCTCTACGGCACCAAATTTGGCGGCACCATCTTCGACAGGTTCGGCCGCCGCCACACCGCCGCCGAGCTTCTCGCCACCGCCGACCCCCATAGACTCACCGTCTTGGTTTATGCCACTGTTCAGAATATTGTATTTGATACGACAGGTATGTTAACCCACTCTGCACACGAACTGTTTGTCGAAATTCTTGTGAGAATGCCTTTGTATGGGAAATGGGAATCCCTCTGTTTTCAATTTTAGCTGTTTGTGCACTTGTTTTCTTTGAACTAGTTGGTAGAGTTATATGCTTTGGCCCACAATTTAGTCCCTGCTTTAAAAAAGTTTAGAATAGTTGCTTGGTTGTGTCAATTGAGTTCATATACTTTTGAAAATCTTTTATAACTTATGATAAATTCTTAAGCCCGTTAGATAACCATTTCGTTTTTGGTTTTTGAAAATTAAGCCTACAAACACTACTTTCACCTTGTGTTGTCATCTATCATCTTTCCATATTTTCAAAAAAGTTTTCAAAGACTTTTTTTTGTTTTTGGAATTTGGCTAGAATGTAAATGTGTCCTTAACAGTGATGAAAACCATTTAAAGAAAATTGGGAGGAAGCAAGCTTAATTTTCAAAAACCAAAACAAAAATCCAAGTGGTTATCAAATGGATCTAAACTTTTCATTTTATGTTTAATAGATTCTTGTAGTTAACTTTGTTAGTTTAGCTTATGTTTTCAGACTTCAAAGCCTATGTAAAATCAGCTTGCTCCTTAATTCTTAAATAGTATTGTCGGTTTGCCATGTAAGCATTAGAGACTTAAAACACAAGATTGAAAGTTCAGGTTTTTAAATTATAAGGATTAGATATATACACCCTTACAATTTTAGGGTCTTAATTAACAAATTCATAGACACAATATTGAAAGTAATTCTGTCTATTTTCTTAAAGGAACTATATATTCTTGATTCTTAAATTCAAAAACAGAAAGAAGAGTATTGTGTGGCAGAGTTGAAATGAAACCATGTTGTTCAAAAATCGTAGTAGTGAACAAAATGATATCAAAATAGAAGTGAATGGGCCAATGTGTTGTGCAGGGAAGCAACCAAAGGCAGTGGGAGTGATCTTTAAGGATGAAAATGGGAATAGACATCAAGCAGTTCTAAGAAATAGGCGGCAGAGTGAGGTTATCTTATCTTCTGGAGCACTTGGGTCACCTCAAATGCTGCTGCTGAGTGGAATTGGGCCAAGAGCAGATCTTGAAAAGTTGAACATATCTGTAGTGCTTGACAATGAGTTTGTTGGGAAAGGGATGGCTGATAACCCCATGAACACTGTCTTTGTTCCCACCAACAGACCTATAGAGAAATCACTTATACAGACTGTTGGGATTACTAAGCTTGGTGTTTACATTGAATCTAGCAGCGGTTTTGGGCAGTCTTCGGATAGCATTCGATGCAATCACGGGATGATGTCAGCTGAAGTAAGCTACCTTTCTTATAAGCATTAGATTCCTGATTTCATCATTTATGATAAAAACGAGTTTTTATTTGCTAGTTATAGGACCAATAATTCATATATTTGTCACATATTTGGAAGAACTGAATGTCAAAAGCATAAGAAACAAAGAAAAATAAATGAAAACATTGCAATCTTTACAAATCATATTCTGGGAGACACATGGAATTTGTTAATGAAAAGTTAGTAACTATTGTATTTGTGATGTAACCAGATTGGGCAACTATCAACCATTCCCCCAAAGCAGAGAACCTGGGAAGCTGTCCAAGCTTACATCAAAAGGAAGCGCGATTTACCTCAGGAGGCATTCCAGGGAGGTTTCATTCTTGAAAAGATTGCCAATCCCATCTCGACAGGGCAGCTTACCTTGGCTAACACCAATGTTGATGACAATCCATCTGTGACTTTCAATTACTTCAGCCACCCGTATGATCTTCACCGGTGTGTCGATGGCATTCGCATGGCAGCAAAAGTTGTGCAGTCAAAACACTTCACTGATTACACAAAAGATACTAGGGAAACCATTGAGAGGCTACTTAATGCGACTGTGAAGGCCAATGTCAATCTCATTCCTAAGCATACCAATGACACCAAGTCTCTAGAGCAGTTCTGCAGAGACACTGTGATCACAATTTGGCATTACCATGGAGGGTGCCATGTGGGCAAGGTCGTGAGCCCTGATTACAAGGTCCTTGGCGTTTCCCGTCTCCGTGTCATCGATGGATCAACCTTTAATGAATCTCCTGGAACCAATCCTCAAGCCACTGTCATGATGATGGGAAGGTAAGGCTAAAAATCAGTTCATGAGTTCATTCAATATTTAGACTACAATTGGTGATTGGTCTTAACAAAAATTAGTAAAAACCGAAGTTAGACATACACATGAAGAATTATGAACTAGCAACTAGCTTTACTCTTGGATGTTCTTATGCTGCAGGTACATGGGGCTGAAGATTTTGAAGGATAGACTGGGAAGGGCAGCTGGTATATAG

mRNA sequence

ATGTGTAGGGTCGAAATCGTATTCGGAATTCCGATACCCGTTTATCAAACGGGCGAGCTCGTTCTCGTCGTTCTCGTCGTCGACGTATTCGTCGCGTCGGAGCGAGAATGTTTACGACTATATAATTGTGGGGGAGGCACGGCGGGGTGTCCCTTGGCAACGACATTGTCGAAAAGATTTAGCGTTTTATTGCTCGAGAGAGGAGGAGTCCCTTTTGCCAATTCAAATGTGTCATTTTTGAAGAACTTTCATATTACTTTGGCTGACATTTCACCCACTTCAGCTTCCCAAGCCTTCATTTCTACTGATGGAGTTCTCAATGCTAGGGCTAGGGTTTTGGGTGGTGGCACTTCCATCAATGCTGGTTTCTACACTAGAGCAAGCTCAAGGTTCATAGAGAAAGTGGGTTGGGACGCGAAGCTTGTGAATGAATCATACCCATGGATCGAGGCTCAGATTGTTCACCGCCCCAAGCTCACGCCATGGCAGAGAGCTTTCAGAGACAGTCTTTTGGACGTCGGTGTCTCCCCCTTCAATGGCTTCACCTACGATCATCTCTACGGCACCAAATTTGGCGGCACCATCTTCGACAGGTTCGGCCGCCGCCACACCGCCGCCGAGCTTCTCGCCACCGCCGACCCCCATAGACTCACCGTCTTGGTTTATGCCACTGTTCAGAATATTGTATTTGATACGACAGGGAAGCAACCAAAGGCAGTGGGAGTGATCTTTAAGGATGAAAATGGGAATAGACATCAAGCAGTTCTAAGAAATAGGCGGCAGAGTGAGGTTATCTTATCTTCTGGAGCACTTGGGTCACCTCAAATGCTGCTGCTGAGTGGAATTGGGCCAAGAGCAGATCTTGAAAAGTTGAACATATCTGTAGTGCTTGACAATGAGTTTGTTGGGAAAGGGATGGCTGATAACCCCATGAACACTGTCTTTGTTCCCACCAACAGACCTATAGAGAAATCACTTATACAGACTGTTGGGATTACTAAGCTTGGTGTTTACATTGAATCTAGCAGCGGTTTTGGGCAGTCTTCGGATAGCATTCGATGCAATCACGGGATGATGTCAGCTGAAATTGGGCAACTATCAACCATTCCCCCAAAGCAGAGAACCTGGGAAGCTGTCCAAGCTTACATCAAAAGGAAGCGCGATTTACCTCAGGAGGCATTCCAGGGAGGTTTCATTCTTGAAAAGATTGCCAATCCCATCTCGACAGGGCAGCTTACCTTGGCTAACACCAATGTTGATGACAATCCATCTGTGACTTTCAATTACTTCAGCCACCCGTATGATCTTCACCGGTGTGTCGATGGCATTCGCATGGCAGCAAAAGTTGTGCAGTCAAAACACTTCACTGATTACACAAAAGATACTAGGGAAACCATTGAGAGGCTACTTAATGCGACTGTGAAGGCCAATGTCAATCTCATTCCTAAGCATACCAATGACACCAAGTCTCTAGAGCAGTTCTGCAGAGACACTGTGATCACAATTTGGCATTACCATGGAGGGTGCCATGTGGGCAAGGTCGTGAGCCCTGATTACAAGGTCCTTGGCGTTTCCCGTCTCCGTGTCATCGATGGATCAACCTTTAATGAATCTCCTGGAACCAATCCTCAAGCCACTGTCATGATGATGGGAAGGTACATGGGGCTGAAGATTTTGAAGGATAGACTGGGAAGGGCAGCTGGTATATAG

Coding sequence (CDS)

ATGTGTAGGGTCGAAATCGTATTCGGAATTCCGATACCCGTTTATCAAACGGGCGAGCTCGTTCTCGTCGTTCTCGTCGTCGACGTATTCGTCGCGTCGGAGCGAGAATGTTTACGACTATATAATTGTGGGGGAGGCACGGCGGGGTGTCCCTTGGCAACGACATTGTCGAAAAGATTTAGCGTTTTATTGCTCGAGAGAGGAGGAGTCCCTTTTGCCAATTCAAATGTGTCATTTTTGAAGAACTTTCATATTACTTTGGCTGACATTTCACCCACTTCAGCTTCCCAAGCCTTCATTTCTACTGATGGAGTTCTCAATGCTAGGGCTAGGGTTTTGGGTGGTGGCACTTCCATCAATGCTGGTTTCTACACTAGAGCAAGCTCAAGGTTCATAGAGAAAGTGGGTTGGGACGCGAAGCTTGTGAATGAATCATACCCATGGATCGAGGCTCAGATTGTTCACCGCCCCAAGCTCACGCCATGGCAGAGAGCTTTCAGAGACAGTCTTTTGGACGTCGGTGTCTCCCCCTTCAATGGCTTCACCTACGATCATCTCTACGGCACCAAATTTGGCGGCACCATCTTCGACAGGTTCGGCCGCCGCCACACCGCCGCCGAGCTTCTCGCCACCGCCGACCCCCATAGACTCACCGTCTTGGTTTATGCCACTGTTCAGAATATTGTATTTGATACGACAGGGAAGCAACCAAAGGCAGTGGGAGTGATCTTTAAGGATGAAAATGGGAATAGACATCAAGCAGTTCTAAGAAATAGGCGGCAGAGTGAGGTTATCTTATCTTCTGGAGCACTTGGGTCACCTCAAATGCTGCTGCTGAGTGGAATTGGGCCAAGAGCAGATCTTGAAAAGTTGAACATATCTGTAGTGCTTGACAATGAGTTTGTTGGGAAAGGGATGGCTGATAACCCCATGAACACTGTCTTTGTTCCCACCAACAGACCTATAGAGAAATCACTTATACAGACTGTTGGGATTACTAAGCTTGGTGTTTACATTGAATCTAGCAGCGGTTTTGGGCAGTCTTCGGATAGCATTCGATGCAATCACGGGATGATGTCAGCTGAAATTGGGCAACTATCAACCATTCCCCCAAAGCAGAGAACCTGGGAAGCTGTCCAAGCTTACATCAAAAGGAAGCGCGATTTACCTCAGGAGGCATTCCAGGGAGGTTTCATTCTTGAAAAGATTGCCAATCCCATCTCGACAGGGCAGCTTACCTTGGCTAACACCAATGTTGATGACAATCCATCTGTGACTTTCAATTACTTCAGCCACCCGTATGATCTTCACCGGTGTGTCGATGGCATTCGCATGGCAGCAAAAGTTGTGCAGTCAAAACACTTCACTGATTACACAAAAGATACTAGGGAAACCATTGAGAGGCTACTTAATGCGACTGTGAAGGCCAATGTCAATCTCATTCCTAAGCATACCAATGACACCAAGTCTCTAGAGCAGTTCTGCAGAGACACTGTGATCACAATTTGGCATTACCATGGAGGGTGCCATGTGGGCAAGGTCGTGAGCCCTGATTACAAGGTCCTTGGCGTTTCCCGTCTCCGTGTCATCGATGGATCAACCTTTAATGAATCTCCTGGAACCAATCCTCAAGCCACTGTCATGATGATGGGAAGGTACATGGGGCTGAAGATTTTGAAGGATAGACTGGGAAGGGCAGCTGGTATATAG

Protein sequence

MCRVEIVFGIPIPVYQTGELVLVVLVVDVFVASERECLRLYNCGGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTDGVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQRAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYATVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTRETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI
Homology
BLAST of Lag0016804 vs. NCBI nr
Match: XP_038879924.1 (protein HOTHEAD [Benincasa hispida])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.3e-298
Identity = 508/526 (96.58%), Postives = 518/526 (98.48%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLATTLSK+F+VLLLERGGVPFANSNVSFL NFHITLADISPTSASQAFISTD
Sbjct: 69  GGGTAGCPLATTLSKKFNVLLLERGGVPFANSNVSFLDNFHITLADISPTSASQAFISTD 128

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDAKLVNESYPWIE QIVHRPKLTPWQ
Sbjct: 129 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAKLVNESYPWIENQIVHRPKLTPWQ 188

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVG+SPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 189 RAFRDSLLDVGISPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 248

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 249 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 308

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSS
Sbjct: 309 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSS 368

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKI
Sbjct: 369 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKI 428

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           ANPISTG+LTL NTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAK+VQSKHFTDYTKDTR
Sbjct: 429 ANPISTGKLTLVNTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKIVQSKHFTDYTKDTR 488

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLG
Sbjct: 489 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLG 548

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRVIDGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLG AAGI
Sbjct: 549 VSRLRVIDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGSAAGI 594

BLAST of Lag0016804 vs. NCBI nr
Match: XP_022148088.1 (protein HOTHEAD-like [Momordica charantia])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.6e-294
Identity = 500/526 (95.06%), Postives = 518/526 (98.48%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLS++FSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD
Sbjct: 71  GGGTAGCPLAATLSQKFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 130

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGG+ INAGFYTRAS RFIEKVGWDAKLVNESYPW+E QIVH PKLTPWQ
Sbjct: 131 GVLNARARVLGGGSCINAGFYTRASWRFIEKVGWDAKLVNESYPWVEKQIVHLPKLTPWQ 190

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 191 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 250

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQNIVFDTTGK+PKAVGVIFKDENGNRHQAVLRNR QSEVILSSGA+G+PQMLLLSGIG
Sbjct: 251 TVQNIVFDTTGKKPKAVGVIFKDENGNRHQAVLRNRGQSEVILSSGAIGTPQMLLLSGIG 310

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLD+EFVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITKLGVYIESSS
Sbjct: 311 PRADLEKLNISVVLDSEFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSS 370

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI
Sbjct: 371 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 430

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A+PISTGQLTLANTNVDDNPSVTFNYF HPYDLHRCVDGIRMAAKVVQSKHFT YTK+TR
Sbjct: 431 ADPISTGQLTLANTNVDDNPSVTFNYFGHPYDLHRCVDGIRMAAKVVQSKHFTKYTKNTR 490

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ++IE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLG
Sbjct: 491 QSIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLG 550

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI
Sbjct: 551 VSRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 596

BLAST of Lag0016804 vs. NCBI nr
Match: XP_023526198.1 (protein HOTHEAD-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1017.7 bits (2630), Expect = 3.9e-293
Identity = 498/526 (94.68%), Postives = 515/526 (97.91%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLSK+F+VLLLERGGVPFANSNVSFL NFHITLADISPTSASQAFISTD
Sbjct: 72  GGGTAGCPLAATLSKKFNVLLLERGGVPFANSNVSFLSNFHITLADISPTSASQAFISTD 131

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDAKLVNESYPWIE QIVHRPKLTPWQ
Sbjct: 132 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAKLVNESYPWIENQIVHRPKLTPWQ 191

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 192 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 251

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TV NIVFDTTGKQPKAVGVIFKDENGNRH+AVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 252 TVLNIVFDTTGKQPKAVGVIFKDENGNRHEAVLRNRRQSEVILSSGALGSPQMLLLSGIG 311

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITK GVYIESSS
Sbjct: 312 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIKKSLIQTVGITKFGVYIESSS 371

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEA QAYIK KRDLPQEAF GGFILEKI
Sbjct: 372 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAAQAYIKMKRDLPQEAFWGGFILEKI 431

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A+P+STGQLTLANTNVDDNP+VTFNYF+HPYDLHRCVDGIRMAAKVVQSKHFTD+TK+TR
Sbjct: 432 ADPLSTGQLTLANTNVDDNPTVTFNYFNHPYDLHRCVDGIRMAAKVVQSKHFTDFTKNTR 491

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLG
Sbjct: 492 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLG 551

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLG+ A +
Sbjct: 552 VSRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGQGADL 597

BLAST of Lag0016804 vs. NCBI nr
Match: KAG7018284.1 (Protein HOTHEAD [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1017.7 bits (2630), Expect = 3.9e-293
Identity = 498/526 (94.68%), Postives = 515/526 (97.91%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLSK+F+VLLLERGGVPFANSNVSFL NFHITLADISPTSASQAFISTD
Sbjct: 72  GGGTAGCPLAATLSKKFNVLLLERGGVPFANSNVSFLSNFHITLADISPTSASQAFISTD 131

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDAKLVNESYPWIE QIVHRPKLTPWQ
Sbjct: 132 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAKLVNESYPWIENQIVHRPKLTPWQ 191

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 192 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 251

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TV NIVFDTTGKQPKAVGVIFKDENGNRH+AVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 252 TVLNIVFDTTGKQPKAVGVIFKDENGNRHEAVLRNRRQSEVILSSGALGSPQMLLLSGIG 311

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITK GVYIESSS
Sbjct: 312 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIKKSLIQTVGITKFGVYIESSS 371

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEA QAYIK KRDLPQEAF GGFILEKI
Sbjct: 372 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAAQAYIKTKRDLPQEAFWGGFILEKI 431

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A+P+STGQLTLANTNVDDNP+VTFNYF+HPYDLHRCVDGIRMAAKVVQSKHFTD+TK+TR
Sbjct: 432 ADPLSTGQLTLANTNVDDNPTVTFNYFNHPYDLHRCVDGIRMAAKVVQSKHFTDFTKNTR 491

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLG
Sbjct: 492 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLG 551

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLG+ A +
Sbjct: 552 VSRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGQGADL 597

BLAST of Lag0016804 vs. NCBI nr
Match: XP_022980572.1 (protein HOTHEAD-like [Cucurbita maxima])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.5e-292
Identity = 497/526 (94.49%), Postives = 515/526 (97.91%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLSK+F+VLLLERGGVPFANSNVSFL NFHITLADISPTSASQAFISTD
Sbjct: 72  GGGTAGCPLAATLSKKFNVLLLERGGVPFANSNVSFLSNFHITLADISPTSASQAFISTD 131

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDAKLVNESYPWIE QIVHRPKLTPWQ
Sbjct: 132 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAKLVNESYPWIENQIVHRPKLTPWQ 191

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 192 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 251

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TV NIVFDTTGKQPKAVGVIFKDENGNRH+AVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 252 TVLNIVFDTTGKQPKAVGVIFKDENGNRHEAVLRNRRQSEVILSSGALGSPQMLLLSGIG 311

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITK GVYIESSS
Sbjct: 312 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIKKSLIQTVGITKFGVYIESSS 371

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEA QAYIK KR+LPQEAF GGFILEKI
Sbjct: 372 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAAQAYIKTKRNLPQEAFWGGFILEKI 431

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A+P+STGQLTLANTNVDDNP+VTFNYF+HPYDLHRCVDGIRMAAKVVQSKHFTD+TK+TR
Sbjct: 432 ADPLSTGQLTLANTNVDDNPTVTFNYFNHPYDLHRCVDGIRMAAKVVQSKHFTDFTKNTR 491

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLG
Sbjct: 492 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLG 551

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLG+ A +
Sbjct: 552 VSRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGQGADL 597

BLAST of Lag0016804 vs. ExPASy Swiss-Prot
Match: Q9S746 (Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.9e-224
Identity = 372/526 (70.72%), Postives = 444/526 (84.41%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLS+ FSVL+LERGGVPF N+NVSFL+NFHI LADIS +SASQAF+STD
Sbjct: 69  GGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLADISASSASQAFVSTD 128

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GV NARARVLGGG+ INAGFY+RA + F+++ GWD KLV ESYPW+E +IVH+PKLT WQ
Sbjct: 129 GVYNARARVLGGGSCINAGFYSRADAAFVKRAGWDPKLVKESYPWVEREIVHQPKLTLWQ 188

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           +A RDSLL+VGV PFNGFTYDH+ GTK GGTIFDRFGRRHTAAELLA A+P +L VL+YA
Sbjct: 189 KALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVLIYA 248

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQ IVFDT+G +P+  GVIFKDE GN+HQA+L NR+ SEVILSSGA+GSPQML+LSGIG
Sbjct: 249 TVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIG 308

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           P+ +L++L I VVL+NE VGKGMADNPMNT+ VP+  PIE+SLIQTVGITK+GVY+E+S+
Sbjct: 309 PKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEAST 368

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQS +SI  ++G+MS +    STIP KQR  EA QAYI R +    EAF G FILEK+
Sbjct: 369 GFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKL 428

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A PIS G L+L NTNVDDNPSVTFNYF HP DL RCV+ IR+ +KVV S  F +YT+  +
Sbjct: 429 AYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDK 488

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           + + ++L+ +VKAN+NL PK  NDTKS+ QFC+DTV+TIWHYHGGC VGKVVSP+ KVLG
Sbjct: 489 QNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLG 548

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           V RLRVIDGSTF+ESPGTNPQAT+MMMGRYMG+KIL++RLG  AG+
Sbjct: 549 VDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKAGV 594

BLAST of Lag0016804 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 4.3e-125
Identity = 250/527 (47.44%), Postives = 327/527 (62.05%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISP-TSASQAFIST 103
           GGGTAGCPLA TLS+ F VLLLERGGVP+   NV     F  TL D++   S +Q+FIS 
Sbjct: 60  GGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISE 119

Query: 104 DGVLNARARVLGGGTSINAGFYTRASSRFIEKVG--WDAKLVNESYPWIEAQIVHRPKLT 163
           +GV NAR RVLGG ++INAGFY+RA  +F E  G  WD   VN+SY W+E  IV RP+L 
Sbjct: 120 EGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLR 179

Query: 164 PWQRAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVL 223
            WQ A RD+LL+VGV PFNGFT +H  GTK GG+ FDR GRRH++A+LL  A    + V 
Sbjct: 180 TWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVA 239

Query: 224 VYATVQNIVF----DTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQM 283
           VYATV+ ++       +G    A+GV+++D+ G  H A++R+R   EVILS+GALGSPQ+
Sbjct: 240 VYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDR--GEVILSAGALGSPQL 299

Query: 284 LLLSGIGPRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLG 343
           L LSGIGPR+ L    I V LD   VG  + DNP N + +    P+E SLIQ VG+T+ G
Sbjct: 300 LFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDG 359

Query: 344 VYIESSSGFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQG 403
            ++E++S           N    ++ +  +    P    +  V                 
Sbjct: 360 AFLEAAS-----------NVIPFASPLHSVFIRAPASPLYVPVTT--------------- 419

Query: 404 GFILEKIANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFT 463
             I+EKI  P+S G L LA+T+V  NP V FNYFS P DL RCV+G R   ++++S+   
Sbjct: 420 --IMEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQ 479

Query: 464 DYTKDTRETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVS 523
           D+    RE         V A    +P   ++   +  FCR TV TIWHYHGG  VGKVV 
Sbjct: 480 DFM--IREWFGNRRFRFVGAP---LPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVD 539

Query: 524 PDYKVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRL 564
            D KV+GV+ LR++DGSTFN SPGTNPQAT+MM+GRYMGLK+L++R+
Sbjct: 540 SDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

BLAST of Lag0016804 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.5e-101
Identity = 204/523 (39.01%), Postives = 294/523 (56.21%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITL-ADISPTSASQAFIST 103
           GGGTAGCPLA TLS  +SVL+LERG +P    N+     F   L  +    +  + F+S 
Sbjct: 60  GGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSE 119

Query: 104 DGVLNARARVLGGGTSINAGFYTRASSRFIEKVG--WDAKLVNESYPWIEAQIVHRPKLT 163
           DG+ N R RVLGG + INAG Y RA++ F  + G  WD  LVN++Y W+E  IV  P   
Sbjct: 120 DGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQ 179

Query: 164 PWQRAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVL 223
            WQ     + L+ G+ P NGF+ DHL GT+  G+ FD  G RH + ELL   DP+ L V 
Sbjct: 180 TWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVA 239

Query: 224 VYATVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLS 283
           V A V+ I+F +      A+GVI+ D NG  HQA +R   + EVILS+G +GSPQ+LLLS
Sbjct: 240 VQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRG--EGEVILSAGPIGSPQLLLLS 299

Query: 284 GIGPRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIE 343
           G+GP + L  LNISVV  + +VG+ + DNP N + +    PIE S +  +GIT       
Sbjct: 300 GVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGIT------- 359

Query: 344 SSSGFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFIL 403
                   SD  +C+   +  +    S  P                  LP + F    I+
Sbjct: 360 --------SDFYQCSISSLPFDTPPFSFFP-------------TTSYPLPNQTF--AHIV 419

Query: 404 EKIANPISTGQLTL-ANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYT 463
            K+  P+S G +TL ++++V   P+V FNY+S+  DL  CV G++   +V+ +     Y 
Sbjct: 420 NKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYK 479

Query: 464 KDTRETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDY 523
            +    I+      +      +P++  D  + E FCR++V + WHYHGGC VGKV+   +
Sbjct: 480 VEDLPGIDGFNILGIP-----LPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGF 539

Query: 524 KVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDR 563
           +V G++ LRV+DGSTF  +P ++PQ   +M+GRYMG++IL++R
Sbjct: 540 RVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQER 545

BLAST of Lag0016804 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.3e-100
Identity = 211/542 (38.93%), Postives = 298/542 (54.98%), Query Frame = 0

Query: 29  VFVASERECLRLYN---CGGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHI 88
           V+ A++ E    Y+    GGGTAGCPLA TLS  +SVL+LERG +P    N+     F  
Sbjct: 43  VYDANDTELEGTYDYIIVGGGTAGCPLAATLSANYSVLVLERGTLPTEYPNLLTSDGFIY 102

Query: 89  TL-ADISPTSASQAFISTDGVLNARARVLGGGTSINAGFYTRASSRFIEKVG--WDAKLV 148
            L  +    +  + F+S DG+ N R RVLGG + INAG Y RA++ F  + G  WD  LV
Sbjct: 103 NLQQEDDGQTPVERFVSGDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLV 162

Query: 149 NESYPWIEAQIVHRPKLTPWQRAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRR 208
           N++Y W+E  IV +P    WQ     + L+VG+ P NGF+ DHL GT+  G+ FD  G R
Sbjct: 163 NKTYDWVEDTIVFKPDFQFWQNLTGTAFLEVGILPDNGFSLDHLEGTRLTGSTFDNNGTR 222

Query: 209 HTAAELLATADPHRLTVLVYATVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQS 268
           H + ELL   DP+ L V V+A V+ I+F +      A+GVI+ D NG  HQA +R     
Sbjct: 223 HASDELLNKGDPNNLRVAVHAAVEKIIFSSDSSGVTAIGVIYTDSNGTTHQAFVRG--DG 282

Query: 269 EVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPI 328
           EVILS+G +GSPQ+LLLSG+G  + L  LNISVV  + +VG+ + DNP N + +    PI
Sbjct: 283 EVILSAGPIGSPQLLLLSGVGLESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPI 342

Query: 329 EKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAY 388
           E S +  +GIT               SD  +C+   +          P            
Sbjct: 343 EASTVTVLGIT---------------SDFYQCSISSLPFSTAPFGFFP------------ 402

Query: 389 IKRKRDLPQEAFQGGFILEKIANPISTGQLTLANT-NVDDNPSVTFNYFSHPYDLHRCVD 448
                 LP   F    I+ K+  P+S G + L +T +V   P+VTFNY+S+  DL  CV 
Sbjct: 403 -NPTYPLPNTTF--AHIVNKVPGPLSHGTVLLQSTSDVRVAPNVTFNYYSNTTDLAHCVS 462

Query: 449 GIRMAAKVVQSKHFTDYTKDTRETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVIT 508
           G++   + + S     Y  +    IE      +      +P++  D  + E FCR+ V +
Sbjct: 463 GMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIP-----LPENQTDDAAFETFCREAVAS 522

Query: 509 IWHYHGGCHVGKVVSPDYKVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKD 564
            WHYHGGC VG+V+  D++V G++ LRV+DGSTF  +P ++PQ   +M+GRYMG KIL++
Sbjct: 523 YWHYHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQE 547

BLAST of Lag0016804 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.1e-99
Identity = 203/523 (38.81%), Postives = 298/523 (56.98%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITL-ADISPTSASQAFIST 103
           GGGT+GCPLA TLS+++ VL+LERG +P A  NV     F   L  +    +  + F+S 
Sbjct: 60  GGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSE 119

Query: 104 DGVLNARARVLGGGTSINAGFYTRASSRFIEKVG--WDAKLVNESYPWIEAQIVHRPKLT 163
           DG+ N R RVLGG + INAG Y RA++      G  WD  LVN++Y W+E  IV +P   
Sbjct: 120 DGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQ 179

Query: 164 PWQRAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVL 223
           PWQ     + L+ GV P +GF+ DH  GT+  G+ FD  G RH A ELL   + + L V 
Sbjct: 180 PWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVG 239

Query: 224 VYATVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLS 283
           V+A+V+ I+F +      A GVI++D NG  H+A +R++   EVI+S+G +G+PQ+LLLS
Sbjct: 240 VHASVEKIIF-SNAPGLTATGVIYRDSNGTPHRAFVRSK--GEVIVSAGTIGTPQLLLLS 299

Query: 284 GIGPRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIE 343
           G+GP + L  LNI VVL + +VG+ + DNP N + +    PIE +++  +GI        
Sbjct: 300 GVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI-------- 359

Query: 344 SSSGFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFIL 403
                  S+D  +C+   +        T PP          +      LP   F      
Sbjct: 360 -------SNDFYQCSFSSLP------FTTPP-------FSFFPSTSYPLPNSTF--AHFA 419

Query: 404 EKIANPISTGQLTL-ANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYT 463
            K+A P+S G LTL +++NV  +P+V FNY+S+P DL  CV G++   +++ +     Y 
Sbjct: 420 SKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYK 479

Query: 464 KDTRETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDY 523
            +    IE      +      +PK   D  + E FCR++V + WHYHGGC VGKV+  D+
Sbjct: 480 VEDLPGIEGFNILGIP-----LPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDF 539

Query: 524 KVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDR 563
           +V G+  LRV+DGSTF  +P ++PQ   +M+GRY+G+KIL++R
Sbjct: 540 RVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Lag0016804 vs. ExPASy TrEMBL
Match: A0A6J1D343 (protein HOTHEAD-like OS=Momordica charantia OX=3673 GN=LOC111016853 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.2e-294
Identity = 500/526 (95.06%), Postives = 518/526 (98.48%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLS++FSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD
Sbjct: 71  GGGTAGCPLAATLSQKFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 130

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGG+ INAGFYTRAS RFIEKVGWDAKLVNESYPW+E QIVH PKLTPWQ
Sbjct: 131 GVLNARARVLGGGSCINAGFYTRASWRFIEKVGWDAKLVNESYPWVEKQIVHLPKLTPWQ 190

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 191 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 250

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQNIVFDTTGK+PKAVGVIFKDENGNRHQAVLRNR QSEVILSSGA+G+PQMLLLSGIG
Sbjct: 251 TVQNIVFDTTGKKPKAVGVIFKDENGNRHQAVLRNRGQSEVILSSGAIGTPQMLLLSGIG 310

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLD+EFVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITKLGVYIESSS
Sbjct: 311 PRADLEKLNISVVLDSEFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSS 370

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI
Sbjct: 371 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 430

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A+PISTGQLTLANTNVDDNPSVTFNYF HPYDLHRCVDGIRMAAKVVQSKHFT YTK+TR
Sbjct: 431 ADPISTGQLTLANTNVDDNPSVTFNYFGHPYDLHRCVDGIRMAAKVVQSKHFTKYTKNTR 490

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ++IE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLG
Sbjct: 491 QSIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLG 550

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI
Sbjct: 551 VSRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 596

BLAST of Lag0016804 vs. ExPASy TrEMBL
Match: A0A6J1IRQ6 (protein HOTHEAD-like OS=Cucurbita maxima OX=3661 GN=LOC111479901 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.2e-293
Identity = 497/526 (94.49%), Postives = 515/526 (97.91%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLSK+F+VLLLERGGVPFANSNVSFL NFHITLADISPTSASQAFISTD
Sbjct: 72  GGGTAGCPLAATLSKKFNVLLLERGGVPFANSNVSFLSNFHITLADISPTSASQAFISTD 131

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDAKLVNESYPWIE QIVHRPKLTPWQ
Sbjct: 132 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAKLVNESYPWIENQIVHRPKLTPWQ 191

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 192 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 251

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TV NIVFDTTGKQPKAVGVIFKDENGNRH+AVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 252 TVLNIVFDTTGKQPKAVGVIFKDENGNRHEAVLRNRRQSEVILSSGALGSPQMLLLSGIG 311

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITK GVYIESSS
Sbjct: 312 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIKKSLIQTVGITKFGVYIESSS 371

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEA QAYIK KR+LPQEAF GGFILEKI
Sbjct: 372 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAAQAYIKTKRNLPQEAFWGGFILEKI 431

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A+P+STGQLTLANTNVDDNP+VTFNYF+HPYDLHRCVDGIRMAAKVVQSKHFTD+TK+TR
Sbjct: 432 ADPLSTGQLTLANTNVDDNPTVTFNYFNHPYDLHRCVDGIRMAAKVVQSKHFTDFTKNTR 491

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLG
Sbjct: 492 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLG 551

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLG+ A +
Sbjct: 552 VSRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGQGADL 597

BLAST of Lag0016804 vs. ExPASy TrEMBL
Match: A0A5A7U5M2 (Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001160 PE=3 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.6e-292
Identity = 495/526 (94.11%), Postives = 512/526 (97.34%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLSK F+VLLLERGGVPFANSNVSFL NFHI LAD+SPTSASQAFISTD
Sbjct: 49  GGGTAGCPLAATLSKNFNVLLLERGGVPFANSNVSFLDNFHIALADLSPTSASQAFISTD 108

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDA LVNESYPWIE QIVHRPKLTPWQ
Sbjct: 109 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAVLVNESYPWIEKQIVHRPKLTPWQ 168

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVG+SPFNGFTYDH+YGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLV+A
Sbjct: 169 RAFRDSLLDVGISPFNGFTYDHIYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVHA 228

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 229 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 288

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSS
Sbjct: 289 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSS 348

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKI
Sbjct: 349 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKI 408

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           ANP+STGQLTLANTNVDDNPSVTFNYF+HPYDLHRC+DGIR AAK+VQSKHF +YTK+T 
Sbjct: 409 ANPLSTGQLTLANTNVDDNPSVTFNYFNHPYDLHRCIDGIRTAAKIVQSKHFREYTKNTG 468

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLG
Sbjct: 469 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLG 528

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTFNESPGTNPQATVMMMGRYMGLKILKDRLG+ AGI
Sbjct: 529 VSRLRVVDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGKTAGI 574

BLAST of Lag0016804 vs. ExPASy TrEMBL
Match: A0A6J1GTL1 (protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111457419 PE=3 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.6e-292
Identity = 497/526 (94.49%), Postives = 514/526 (97.72%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLSK+F+VLLLERGGVPFANSNVSFL NFHITLADISPTSASQAFISTD
Sbjct: 72  GGGTAGCPLAATLSKKFNVLLLERGGVPFANSNVSFLSNFHITLADISPTSASQAFISTD 131

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDAKLVNESYPWIE QIVHRPKLTPWQ
Sbjct: 132 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAKLVNESYPWIENQIVHRPKLTPWQ 191

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLVYA
Sbjct: 192 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVYA 251

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TV NIVFDTTGKQPKAVGVIFKDENGNRH+AVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 252 TVLNIVFDTTGKQPKAVGVIFKDENGNRHEAVLRNRRQSEVILSSGALGSPQMLLLSGIG 311

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITK GVYIESSS
Sbjct: 312 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIKKSLIQTVGITKFGVYIESSS 371

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEA QAYIK KRDLPQEAF GGFILEKI
Sbjct: 372 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAAQAYIKTKRDLPQEAFWGGFILEKI 431

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A+P+STGQLTLANTNVDDNP+VTFNYF+HPYDLHRCVDGIRMAAKVVQSKHFT +TK+TR
Sbjct: 432 ADPLSTGQLTLANTNVDDNPTVTFNYFNHPYDLHRCVDGIRMAAKVVQSKHFTAFTKNTR 491

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLG
Sbjct: 492 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLG 551

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTF+ESPGTNPQATVMMMGRYMGLKILKDRLG+ A +
Sbjct: 552 VSRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKILKDRLGQGADL 597

BLAST of Lag0016804 vs. ExPASy TrEMBL
Match: A0A1S3BH95 (protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103490022 PE=3 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.6e-292
Identity = 495/526 (94.11%), Postives = 512/526 (97.34%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLSK F+VLLLERGGVPFANSNVSFL NFHI LAD+SPTSASQAFISTD
Sbjct: 70  GGGTAGCPLAATLSKNFNVLLLERGGVPFANSNVSFLDNFHIALADLSPTSASQAFISTD 129

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GVLNARARVLGGGT INAGFYTRASSRFIEKVGWDA LVNESYPWIE QIVHRPKLTPWQ
Sbjct: 130 GVLNARARVLGGGTCINAGFYTRASSRFIEKVGWDAVLVNESYPWIEKQIVHRPKLTPWQ 189

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           RAFRDSLLDVG+SPFNGFTYDH+YGTKFGGTIFDRFGRRHTAAELLATADPH+LTVLV+A
Sbjct: 190 RAFRDSLLDVGISPFNGFTYDHIYGTKFGGTIFDRFGRRHTAAELLATADPHKLTVLVHA 249

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG
Sbjct: 250 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 309

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSS
Sbjct: 310 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSS 369

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKI
Sbjct: 370 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKI 429

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           ANP+STGQLTLANTNVDDNPSVTFNYF+HPYDLHRC+DGIR AAK+VQSKHF +YTK+T 
Sbjct: 430 ANPLSTGQLTLANTNVDDNPSVTFNYFNHPYDLHRCIDGIRTAAKIVQSKHFREYTKNTG 489

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           ETIE+LLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLG
Sbjct: 490 ETIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLG 549

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           VSRLRV+DGSTFNESPGTNPQATVMMMGRYMGLKILKDRLG+ AGI
Sbjct: 550 VSRLRVVDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGKTAGI 595

BLAST of Lag0016804 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 778.1 bits (2008), Expect = 4.9e-225
Identity = 372/526 (70.72%), Postives = 444/526 (84.41%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLS+ FSVL+LERGGVPF N+NVSFL+NFHI LADIS +SASQAF+STD
Sbjct: 69  GGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLADISASSASQAFVSTD 128

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GV NARARVLGGG+ INAGFY+RA + F+++ GWD KLV ESYPW+E +IVH+PKLT WQ
Sbjct: 129 GVYNARARVLGGGSCINAGFYSRADAAFVKRAGWDPKLVKESYPWVEREIVHQPKLTLWQ 188

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           +A RDSLL+VGV PFNGFTYDH+ GTK GGTIFDRFGRRHTAAELLA A+P +L VL+YA
Sbjct: 189 KALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVLIYA 248

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQ IVFDT+G +P+  GVIFKDE GN+HQA+L NR+ SEVILSSGA+GSPQML+LSGIG
Sbjct: 249 TVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIG 308

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           P+ +L++L I VVL+NE VGKGMADNPMNT+ VP+  PIE+SLIQTVGITK+GVY+E+S+
Sbjct: 309 PKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEAST 368

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQS +SI  ++G+MS +    STIP KQR  EA QAYI R +    EAF G FILEK+
Sbjct: 369 GFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKL 428

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A PIS G L+L NTNVDDNPSVTFNYF HP DL RCV+ IR+ +KVV S  F +YT+  +
Sbjct: 429 AYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDK 488

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           + + ++L+ +VKAN+NL PK  NDTKS+ QFC+DTV+TIWHYHGGC VGKVVSP+ KVLG
Sbjct: 489 QNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLG 548

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           V RLRVIDGSTF+ESPGTNPQAT+MMMGRYMG+KIL++RLG  AG+
Sbjct: 549 VDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKAGV 594

BLAST of Lag0016804 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 736.1 bits (1899), Expect = 2.1e-212
Identity = 359/526 (68.25%), Postives = 426/526 (80.99%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLS+ FSVL+LERGGVPF N+NVSFL+NFHI LADIS +SASQAF+STD
Sbjct: 69  GGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLADISASSASQAFVSTD 128

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GV NARARVLGGG+ INAGFY+RA + F+++ GWD KLV ESYPW+E +IVH+PKLT WQ
Sbjct: 129 GVYNARARVLGGGSCINAGFYSRADAAFVKRAGWDPKLVKESYPWVEREIVHQPKLTLWQ 188

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
           +A RDSLL+VGV PFNGFTYDH+ GTK GGTIFDRFGRRHTAAELLA A+P +L VL+YA
Sbjct: 189 KALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVLIYA 248

Query: 224 TVQNIVFDTTGKQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIG 283
           TVQ IVFDT+G +P+  GVIFKDE GN+HQA+L NR+ SEVILSSGA+GSPQML+LSGIG
Sbjct: 249 TVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIG 308

Query: 284 PRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESSS 343
           P+ +L++L I VVL+NE VGKGMADNPMNT+ VP+  PIE+SLIQTVGITK+GVY+E+S+
Sbjct: 309 PKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEAST 368

Query: 344 GFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKI 403
           GFGQS +SI  ++G+MS +    STIP KQR  EA QAYI R +    EAF G FILEK+
Sbjct: 369 GFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKL 428

Query: 404 ANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHFTDYTKDTR 463
           A PIS G L+L NTNVDDNPSVTFNYF HP D                           +
Sbjct: 429 AYPISRGHLSLVNTNVDDNPSVTFNYFKHPCD---------------------------K 488

Query: 464 ETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLG 523
           + + ++L+ +VKAN+NL PK  NDTKS+ QFC+DTV+TIWHYHGGC VGKVVSP+ KVLG
Sbjct: 489 QNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLG 548

Query: 524 VSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRLGRAAGI 570
           V RLRVIDGSTF+ESPGTNPQAT+MMMGRYMG+KIL++RLG  AG+
Sbjct: 549 VDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKAGV 567

BLAST of Lag0016804 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 550.1 bits (1416), Expect = 2.2e-156
Identity = 289/533 (54.22%), Postives = 361/533 (67.73%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGTAGCPLA TLS+  SVLLLERG  P+ N N++ L  F   L+D+S +S SQ F+S D
Sbjct: 52  GGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSESSPSQRFVSED 111

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GV+NARARVLGGG+++NAGFYTRA ++++  +GWD  L NESY W+EA++  +P +  WQ
Sbjct: 112 GVINARARVLGGGSALNAGFYTRAGTKYVRNMGWDGALANESYQWVEAKVAFQPPMGRWQ 171

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
            A RD LL+ G+ P NGFTYDH+ GTKFGGTIFDR G RHTAA+LL  ADP  +TVL++A
Sbjct: 172 TAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHA 231

Query: 224 TVQNIVFDTTG-KQPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGI 283
           TV  I+F T G  +P A GV+++D  G  H+A L+    SE+ILS+G LGSPQ+L+LSG+
Sbjct: 232 TVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGV 291

Query: 284 GPRADLEKLNISVVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIESS 343
           GP A L+  NI+VV+D   VG+GM DNPMN VFVP+  P+E SLI+ VGIT  G Y+E++
Sbjct: 292 GPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAA 351

Query: 344 SG--FG------QSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAF 403
            G  FG        S S R  + M S          P+    E+  +  K     P   F
Sbjct: 352 GGENFGGGGGGSSGSSSTRDYYAMFS----------PRATLLES-NSMTKLSSAQP---F 411

Query: 404 QGGFILEKIANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKH 463
           QGGF+LEK+  P+STG L L   N  DNP VTFNYF HP DL RCV GI+   +VVQSK 
Sbjct: 412 QGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKA 471

Query: 464 FTDYTKDTRETIERLLNATVKANVNLIPKHTNDTKSL----EQFCRDTVITIWHYHGGCH 523
           F+ Y K    + E LLN T    VNL P  +    SL    E+FC+ TV TIWHYHGGC 
Sbjct: 472 FSRY-KYADVSFEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCV 531

Query: 524 VGKVVSPDYKVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDRL 564
           VG+VV  DYKV+G+ RLRVID ST    PGTNPQATVMM+GRYMG+KIL++RL
Sbjct: 532 VGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGRYMGVKILRERL 569

BLAST of Lag0016804 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 3.3e-149
Identity = 273/527 (51.80%), Postives = 348/527 (66.03%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGT+GC LA TLS+  SVL+LERGG P+ N   + ++NF  TL++ SP S SQ FIS D
Sbjct: 52  GGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFATTLSNTSPKSWSQLFISED 111

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GV N RARVLGGG+ +NAGFYTRA   ++++  W    V  +Y W+E ++  +P +  WQ
Sbjct: 112 GVYNTRARVLGGGSVLNAGFYTRAGDEYVKETEWKTDEVEAAYEWVEKKVAFQPPVLGWQ 171

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
            AF+D LL+ G  P+NGFTYDH+YGTK GGTIFDR G RHTAA+LL  A+P  + V ++A
Sbjct: 172 TAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHA 231

Query: 224 TVQNIVFDTTGK-QPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGI 283
           +V  I+F T G+ +PKA GVIF+D NG  H+A L     +EVILS+GA+GSPQ+L+LSGI
Sbjct: 232 SVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGI 291

Query: 284 GPRADLEKLNIS-VVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIES 343
           GP A L    I  +VLD+  VG+GM DNPMN +F+P+  P+E SLIQ VGITK   YIE 
Sbjct: 292 GPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEG 351

Query: 344 SSGFGQSSDSIR------CNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQ 403
           +SG   S    R       N+          ST  P   T +++  +      L     +
Sbjct: 352 ASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLST-QSITDFFNPINPLLNATTR 411

Query: 404 GGFILEKIANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHF 463
            G IL+KIA PIS G L L NTN DDNPSV FNY+  P DL  CV+GI    KV+ SK F
Sbjct: 412 AGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAF 471

Query: 464 TDYTKDTRETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVV 523
           + + K    TI  LL+  +    NL P+H     +L QFC DTV+TIWHYHGGC VG+VV
Sbjct: 472 SKF-KYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVV 531

Query: 524 SPDYKVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDR 563
             +Y+VLG+  LRVIDGSTF +SPGTNPQATVMM+GRYMG +IL++R
Sbjct: 532 DKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMGQRILQER 576

BLAST of Lag0016804 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 3.3e-149
Identity = 273/527 (51.80%), Postives = 348/527 (66.03%), Query Frame = 0

Query: 44  GGGTAGCPLATTLSKRFSVLLLERGGVPFANSNVSFLKNFHITLADISPTSASQAFISTD 103
           GGGT+GC LA TLS+  SVL+LERGG P+ N   + ++NF  TL++ SP S SQ FIS D
Sbjct: 19  GGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFATTLSNTSPKSWSQLFISED 78

Query: 104 GVLNARARVLGGGTSINAGFYTRASSRFIEKVGWDAKLVNESYPWIEAQIVHRPKLTPWQ 163
           GV N RARVLGGG+ +NAGFYTRA   ++++  W    V  +Y W+E ++  +P +  WQ
Sbjct: 79  GVYNTRARVLGGGSVLNAGFYTRAGDEYVKETEWKTDEVEAAYEWVEKKVAFQPPVLGWQ 138

Query: 164 RAFRDSLLDVGVSPFNGFTYDHLYGTKFGGTIFDRFGRRHTAAELLATADPHRLTVLVYA 223
            AF+D LL+ G  P+NGFTYDH+YGTK GGTIFDR G RHTAA+LL  A+P  + V ++A
Sbjct: 139 TAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHA 198

Query: 224 TVQNIVFDTTGK-QPKAVGVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGI 283
           +V  I+F T G+ +PKA GVIF+D NG  H+A L     +EVILS+GA+GSPQ+L+LSGI
Sbjct: 199 SVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGI 258

Query: 284 GPRADLEKLNIS-VVLDNEFVGKGMADNPMNTVFVPTNRPIEKSLIQTVGITKLGVYIES 343
           GP A L    I  +VLD+  VG+GM DNPMN +F+P+  P+E SLIQ VGITK   YIE 
Sbjct: 259 GPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEG 318

Query: 344 SSGFGQSSDSIR------CNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQ 403
           +SG   S    R       N+          ST  P   T +++  +      L     +
Sbjct: 319 ASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLST-QSITDFFNPINPLLNATTR 378

Query: 404 GGFILEKIANPISTGQLTLANTNVDDNPSVTFNYFSHPYDLHRCVDGIRMAAKVVQSKHF 463
            G IL+KIA PIS G L L NTN DDNPSV FNY+  P DL  CV+GI    KV+ SK F
Sbjct: 379 AGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAF 438

Query: 464 TDYTKDTRETIERLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVV 523
           + + K    TI  LL+  +    NL P+H     +L QFC DTV+TIWHYHGGC VG+VV
Sbjct: 439 SKF-KYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVV 498

Query: 524 SPDYKVLGVSRLRVIDGSTFNESPGTNPQATVMMMGRYMGLKILKDR 563
             +Y+VLG+  LRVIDGSTF +SPGTNPQATVMM+GRYMG +IL++R
Sbjct: 499 DKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMGQRILQER 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879924.15.3e-29896.58protein HOTHEAD [Benincasa hispida][more]
XP_022148088.14.6e-29495.06protein HOTHEAD-like [Momordica charantia][more]
XP_023526198.13.9e-29394.68protein HOTHEAD-like [Cucurbita pepo subsp. pepo][more]
KAG7018284.13.9e-29394.68Protein HOTHEAD [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022980572.11.5e-29294.49protein HOTHEAD-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9S7466.9e-22470.72Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1[more]
Q9SSM24.3e-12547.44(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
P527071.5e-10139.01(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O500481.3e-10038.93(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
P527061.1e-9938.81(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D3432.2e-29495.06protein HOTHEAD-like OS=Momordica charantia OX=3673 GN=LOC111016853 PE=3 SV=1[more]
A0A6J1IRQ67.2e-29394.49protein HOTHEAD-like OS=Cucurbita maxima OX=3661 GN=LOC111479901 PE=3 SV=1[more]
A0A5A7U5M21.6e-29294.11Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863... [more]
A0A6J1GTL11.6e-29294.49protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111457419 PE=3 SV=1[more]
A0A1S3BH951.6e-29294.11protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103490022 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G72970.14.9e-22570.72Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.22.1e-21268.25Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.12.2e-15654.22Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.13.3e-14951.80Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.23.3e-14951.80Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.410.40coord: 155..501
e-value: 5.4E-154
score: 515.7
NoneNo IPR availablePIRSRPIRSR000137-2PIRSR000137-2coord: 43..562
e-value: 1.1E-117
score: 392.0
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 42..569
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 306..508
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 406..552
e-value: 4.7E-29
score: 101.7
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 44..310
e-value: 3.3E-25
score: 89.1
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 269..283
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 110..133
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 43..551
e-value: 5.4E-154
score: 515.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 44..561
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 43..568
e-value: 9.0E-104
score: 346.3
IPR031206Protein Hothead-likePANTHERPTHR45968:SF5PROTEIN HOTHEADcoord: 42..569

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016804.1Lag0016804.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046593 mandelonitrile lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors