Lag0016800 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0016800
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	3-epi-6-deoxocathasterone 23-monooxygenase
Location	chr12: 41363118 .. 41368563 (-)
RNA-Seq Expression	Lag0016800
Synteny	Lag0016800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCTTTCTGGTTTTTTGTGCAGCTCATCACAGCTGCAGCTTTCATAATATCTTCCACAATCATAATCTTCAAAACCTTCATCTCCTCCCCTCCTTCCCTTCCTTCCGGCTCCCTCGGCCTCCCCGTCGTCGGTGAAACCCTCCGCTTCATCGCCTCCGCCTACTCTCCCCGTCCCGAGACCTTCATGGACATACGTCGCCGTCGGTGAGTTTCAAGGGTAGAGCCAGAAATTTTCATTAACTTCTACTTCTGTATAACTGTCACTCTCCTTCCTTCTTCGGTCTGCCCTGATCTCGAGTTCCCGATATCGAGCGCTTAAGAACATGGAAAGATGTTTTTTTTGTAATGGGGTTTTTGTGTTTTGTAGGTATGGGAATGTTTTCAAGTCCCATATATTTGGAAGTCCAACAATTGTTTCAACTGATGCTGAAGTGAGTAGGTTTATTCTACAAAGTGATGCAAAGGCTTTTGTCCCATCTTATCCAAAATCTTTGACAGAATTGATGGGGAAGTCTTCTATTTTGCTCATCAATGGCACTTTTCATAGGAAAATTCATGGCCTCATTGGAGCTTTCTTCAAATCATCTCATCTCAAAGCTCAATTAACCATAGACTTGCAAAAATATCTTCAAAATTCCATCTCTATTTGGAGTTCTTCTCATCACAATCCTATCCACATACAAGATGAAGCCAAAAATGTACCTTCCCTCTCTCTCTCTAAAATTTATCATCATCCAACATATGGGTCGGGATTAAATTTATCATAGCCTATCAGCTTAAATTTTTCGAATTTTTTTAGTAGCGGTTAAAATTTTTGCTCTCATTTGAAACATGGGGTTGGTGATAATTTTGGTTTGAATCATATTGTCTAAGAATTCACATTCATAATCATCAGTCAAATCTTTTTATTTTTATATTTCATTCTTCAGATTGCCTTTGAAGTACTTGTGAAAACATTGATCAGTTTGGAGCCTGGTGAAGAAATGGAATTTTTGAAGAAACAATTTCAAGAATTCATTGCTGGGCTAATGGCTTTCCCAATAAACATCCCAGGAAGCAGACTTTACAGATCATTACAGGCAAGTTTTTGCCATTCGCTTAATGAAAATTTGTGTTCTCTCATCATTGGAAAAACAACAAAAGAAAGTCAAACAATTGGTGTTTTGTGTTTTGTGTTTTGTCTCAAAATCTTCAGGCCAAAAGGAAAATGGTTGGATTTGTGCAAGAAATGATACAATCTAGAAGAAAGAAGATGAAAGAAAATTTGAAAAATATAGTTCTTCCAAAGGATGTTGTGGATGTTTTGTTGAATGATGGAAGCCAACAAGTTACAGATGAACTGATAGCAGAAAATATGATTGATATGATGATTCCAGGCCATGATTCAGTCCCAATCCTCATAACTTTGGCCATAAAATACCTTTCTGATTGTCCATCTGTCCTTCAACATTTGACGGTACGTGTATATCGTTTCCGCTCGTCTTAGCCCGACGTTAAACATTGTTTTTTTAGTCTTTAAACTTTCATATTTGTGTTTGATAGGTTCTTGAACTTTCAAAAGTATCCAACTTTAAAAATGCCAGATAGGTTTATAAGTTTTCAATTTTATGTCTAATAGATCACTGTAGAATTTTTCAAAATTAATGAATCTATTATACATAAATTGGACTTTGTACCTAAGAAGACATTAAAAATTATCTAATAGGACTTTGAACTTTAAAAATGTCAAATAGGTCTATGAACTTTTAATTTTGTGTCTAATAGGTCACTGACATACTTGAGATTTTTTTAAAATGAATAAATCTATTATACATAAAGTTGAATTTTTTTGTATCTAATAAAACATTAAACTTTCAATTTAGTGTCTAATAAGTTTTTAAATTTTAAACAATGTCGAGTACTAGGTCCAAAGACCCTATTAGACTCAAAATTAAGAAAGCTTAAAGATTTATTAAATGGAAAACCAAAATATAAGTATTAGACTCTTTTTTAAGTTCAGAGGCTAGGTTACAAAATTGACAATTCAACGATCTATTATAAATTTTTAAAGTTTAGAGACTGAACTTGTAATTTAACCTCCAGTTTAAAATTATATTCCGTTTGAAATCATAAATTCTTACGTTTTAAGATGTGAGATAGTAATATATGTCTTATATATTCAAATTGACACTATTTATGTATTACTTTTTTTTAAAAAAAAAAAAAGTGTCACAATAATAGAAATGGAATGTTTGAATTTACTTTTAATTAAGTAATTTTTCTTTCTTTATTAAGTGTGTTTGTGTCCCTTATGATGTGTGTTTTATTTGATAGGGATTGTTTCTGTCATTTGTGTGACAACCACATCAATAATAATAGAGAAATACCTCTCTCCCTCTCTCTCTCCACTCTCCAATAATTATGATGATACCTTTTTTCTTTTGTTTTTCTTTCTTTTTCAAGATATATCCTCTTATTTTTCCAAACAATTTTCCAAAAAATTTAAACTATATAAAATAATTAAAAGATCATGAAAGACAGATAGATATATTCATGAATGTAGAAATGTCCACAACAATCATTTTCTTTTGTCTTATAAGGATATTTCAACAGTCAAACAACCCAAAAAATTTAAATTATTGAGTTTTTGTATATATGTATTGGAATATTCTCTTAACCAACAATTTTTTTTTTAATTTGTAATTTTTCATGGGCAGGAAGAAAACATGGAGTTGAAAAAACTGAAAACTCAGCTTGGAGAGCCATTGAAATGGAATGATTACTTGTCATTACCATTTACACAAAATGTAAGAACACTTCCCGAGTAAACAAAAACAACGACGTCGTTATAAACAGTTTTATAATGATGATCAGTTTCGTTCTGGAATTGAATCTTTGACTTTTTTGGTTTAAACTAGACGATAACATCATAGCAAATACTATTTACAATGATGATGAAAGATGTTTGAATAGTCCGAGTATGAACGTGACTTTTTTTTAATCGTTGCAAATATTTAGGTCATATCGGAGACATTAAGGATGGGAAACATTATAACAGGAGTGATGAGAAAGGCCATGAAAGACATTGAAATAAAAGGGCATTTGATACCTAAAGGATGGTGTGTCCTTATATATTTTAGGTCAGTTCATCTTGATGAAAAGCACTTTGAAAGCCCTTACAATTTCAATCCATGGAGGTGGCAAGTAAGTACTTATTGCCCTAATTTATCCTTTTGGTTTCTTCATGTGCCCTAATTTTTTGGGGCTTTCTTTAACTTTTTTGTGGTTAATTTCTTAGAATAAAGACTCAAACAGCTCTAATTTCACTCCTTTTGGAGGGGGATTAAGATTGTGTCCTGGACTTGACTTGGCTAGATTGGAAGCTTCCATTTTCTTACACCATTTTGTCACTGAATTCAGGTAAATAAACATATATATTCATATGAATTATCACTTAATCTATCAATAACCTTCATTTTCGGTAGTTTAATATCGTTGAATCGTCGTTCGGTTTTTTTAATTTGAATCGTATATCTCTCTTGTTGATGTTAGGGTTAATTACTTTGTTTTTAGGGTTTCTATGAAACTCTCCTCTTAGCTAATAAAAAAAAAGGCTAGTTTTTAAGTAACATAAAAGTAGTTTTGAAATATATATTATTTGTTTTCTAAATTGACTAATATAAATAGAAAGCTCATTAAACATCCCAATTTATACCCATACAAAATTATGTCCATATCCATGCTTTTAAATCAACCATCACTATTCACTAATTAAAAATCAGGGGTTTAAGCAAAATCATTAGGGTTTTCAGCAAAATTTCCAAAGTGGGTCACAAGTAATTAACATAATGTCTCTAATTAATGTGGCAATTGGCCAAAAGATAAATCATGCAAGAGAAAGTGAAGCAATCAAGTGATCTTTTTTTTTTTTTGGTGGAAAACATAGAAAAATAATGAAGTGCTGAAATTTACATTAAAAAAAAAGATGGGTTTTTTTTTTTTTTTTTAGGTGAATGATTTGTGGGTCAGGATTGAGTTATTGAATTGAATTGAAAAATCCAAAGAGAGTTTTGGAGTAGAAAGTTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCAAGTTTCTTCAAGTTGGATTTCAACCACATTTTGACTGGTTGATTGAAAAATCCAAAAGAGATGATGTTGGAGTGTTCCAATTAAATTGGTTGAATCCTTATTTTCTCTGATCTAATGTCATGTCTGCTGTCCTTTTTTCTGTCTCTATACAAACACACACGTGCGGTGGGAGACATGTCCTTTTCTTCCATCTCTATGAACACACACACATGTGCACAAAGAGAGAGAGAGAGAGAATCTTTGAGTTTTCTTTTGAGAGGAACACTACTATGTTATGTAACTTTGCTACATATAGTTAGGGTTTCATTTGAGAGGAACTTGGTTTGGTTTCGTCTCGTTAGGAAAGAACCTGGTTGTGTAACTTTGCAACGTATAATTAGGGTTTCGATTTTGTTTGAGAGGTTGAAACTGGGTTATGTAAAAAATTAGGGTTTTGTTCGAAAGAAACTTGATGTGTATGATTAGATTAGAGAAAGGAACTGAGTTATATATAGAATTAAAGTTTTGTTTGAAGGGGATAATCCTCTTGTATACCGTAATTAGGGTTTTGTTGGAGAGAAAGTTACTATGTATATATATATATAGATATAATTAGGGTTTTGTTTGGGAAGAACTTAATTAACTAGATATATTATTAGAGAGGGGAAGATATTAAACTTGCTACCCTACAAGGCTTTTTAGTATTATAAGGTTAAGCAGGAGTGGATGTACTTGAACCACTTACTTCAGGGAGAGGTTACTAATGTATAGACTATTAAGTTATGATCAACTTAACTTGCCATATATACCGGAATTATTGAGATCAGAAACTTGTCCTATATAGTTAGGGTTTAAATTTTCATTCGAAGGAACTTGCTCGGTACAGTTGGGTTTTTTGTTGGATAATGATTTCATTTTTTTAAAAAAAATTAGGTTTCTTGTGTACTAGAAAAAAAGAGTTTAATTATTTCTTACTTTTTCCGCAATTTATTTAATTATTTTTGAATTTCTTTTCTTAAAATATAAAGAAAAAGATCATTTTATCGCTTTAATATAGTACAATTTAGTCCCTAACCCTAATCTTGAATCTCTGCCCTTACAACGTAGATACAAACCTTTTGAACGTATCATAACAGAAAAAGAACATACTACAACACCAAGAACATAAACATTACGAAGTTTAATCAACCCGTCTTTACCCAATGTGACTATATATTTATATTAATGTTTTTGTAATTTGATTAGGTGGGTGGCTGAGGAAGACAAGATTATCAACTTCCCAACAGTAAGAATGAAGAAAGGAATGGCAGTTTGGGTAAAGAAGAGAGAAGACAACTCTTGA

mRNA sequence

ATGGATTCTTTCTGGTTTTTTGTGCAGCTCATCACAGCTGCAGCTTTCATAATATCTTCCACAATCATAATCTTCAAAACCTTCATCTCCTCCCCTCCTTCCCTTCCTTCCGGCTCCCTCGGCCTCCCCGTCGTCGGTGAAACCCTCCGCTTCATCGCCTCCGCCTACTCTCCCCGTCCCGAGACCTTCATGGACATACGTCGCCGTCGGTATGGGAATGTTTTCAAGTCCCATATATTTGGAAGTCCAACAATTGTTTCAACTGATGCTGAAGTGAGTAGGTTTATTCTACAAAGTGATGCAAAGGCTTTTGTCCCATCTTATCCAAAATCTTTGACAGAATTGATGGGGAAGTCTTCTATTTTGCTCATCAATGGCACTTTTCATAGGAAAATTCATGGCCTCATTGGAGCTTTCTTCAAATCATCTCATCTCAAAGCTCAATTAACCATAGACTTGCAAAAATATCTTCAAAATTCCATCTCTATTTGGAGTTCTTCTCATCACAATCCTATCCACATACAAGATGAAGCCAAAAATGCCAAAAGGAAAATGGTTGGATTTGTGCAAGAAATGATACAATCTAGAAGAAAGAAGATGAAAGAAAATTTGAAAAATATAGTTCTTCCAAAGGATGTTGTGGATGTTTTGTTGAATGATGGAAGCCAACAAGTTACAGATGAACTGATAGCAGAAAATATGATTGATATGATGATTCCAGGCCATGATTCAGTCCCAATCCTCATAACTTTGGCCATAAAATACCTTTCTGATTGTCCATCTGTCCTTCAACATTTGACGGAAGAAAACATGGAGTTGAAAAAACTGAAAACTCAGCTTGGAGAGCCATTGAAATGGAATGATTACTTGTCATTACCATTTACACAAAATGTCATATCGGAGACATTAAGGATGGGAAACATTATAACAGGAGTGATGAGAAAGGCCATGAAAGACATTGAAATAAAAGGGCATTTGATACCTAAAGGATGGTGTGTCCTTATATATTTTAGGTCAGTTCATCTTGATGAAAAGCACTTTGAAAGCCCTTACAATTTCAATCCATGGAGGTGGCAAAATAAAGACTCAAACAGCTCTAATTTCACTCCTTTTGGAGGGGGATTAAGATTGTGTCCTGGACTTGACTTGGCTAGATTGGAAGCTTCCATTTTCTTACACCATTTTGTCACTGAATTCAGGTGGGTGGCTGAGGAAGACAAGATTATCAACTTCCCAACAGTAAGAATGAAGAAAGGAATGGCAGTTTGGGTAAAGAAGAGAGAAGACAACTCTTGA

Coding sequence (CDS)

ATGGATTCTTTCTGGTTTTTTGTGCAGCTCATCACAGCTGCAGCTTTCATAATATCTTCCACAATCATAATCTTCAAAACCTTCATCTCCTCCCCTCCTTCCCTTCCTTCCGGCTCCCTCGGCCTCCCCGTCGTCGGTGAAACCCTCCGCTTCATCGCCTCCGCCTACTCTCCCCGTCCCGAGACCTTCATGGACATACGTCGCCGTCGGTATGGGAATGTTTTCAAGTCCCATATATTTGGAAGTCCAACAATTGTTTCAACTGATGCTGAAGTGAGTAGGTTTATTCTACAAAGTGATGCAAAGGCTTTTGTCCCATCTTATCCAAAATCTTTGACAGAATTGATGGGGAAGTCTTCTATTTTGCTCATCAATGGCACTTTTCATAGGAAAATTCATGGCCTCATTGGAGCTTTCTTCAAATCATCTCATCTCAAAGCTCAATTAACCATAGACTTGCAAAAATATCTTCAAAATTCCATCTCTATTTGGAGTTCTTCTCATCACAATCCTATCCACATACAAGATGAAGCCAAAAATGCCAAAAGGAAAATGGTTGGATTTGTGCAAGAAATGATACAATCTAGAAGAAAGAAGATGAAAGAAAATTTGAAAAATATAGTTCTTCCAAAGGATGTTGTGGATGTTTTGTTGAATGATGGAAGCCAACAAGTTACAGATGAACTGATAGCAGAAAATATGATTGATATGATGATTCCAGGCCATGATTCAGTCCCAATCCTCATAACTTTGGCCATAAAATACCTTTCTGATTGTCCATCTGTCCTTCAACATTTGACGGAAGAAAACATGGAGTTGAAAAAACTGAAAACTCAGCTTGGAGAGCCATTGAAATGGAATGATTACTTGTCATTACCATTTACACAAAATGTCATATCGGAGACATTAAGGATGGGAAACATTATAACAGGAGTGATGAGAAAGGCCATGAAAGACATTGAAATAAAAGGGCATTTGATACCTAAAGGATGGTGTGTCCTTATATATTTTAGGTCAGTTCATCTTGATGAAAAGCACTTTGAAAGCCCTTACAATTTCAATCCATGGAGGTGGCAAAATAAAGACTCAAACAGCTCTAATTTCACTCCTTTTGGAGGGGGATTAAGATTGTGTCCTGGACTTGACTTGGCTAGATTGGAAGCTTCCATTTTCTTACACCATTTTGTCACTGAATTCAGGTGGGTGGCTGAGGAAGACAAGATTATCAACTTCCCAACAGTAAGAATGAAGAAAGGAATGGCAGTTTGGGTAAAGAAGAGAGAAGACAACTCTTGA

Protein sequence

MDSFWFFVQLITAAAFIISSTIIIFKTFISSPPSLPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWSSSHHNPIHIQDEAKNAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLNDGSQQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEEDKIINFPTVRMKKGMAVWVKKREDNS

Homology

[Close]

BLAST of Lag0016800 vs. NCBI nr
Match: KAG6581856.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 706.4 bits (1822), Expect = 1.5e-199
Identity = 344/423 (81.32%), Postives = 381/423 (90.07%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFISSPPSLPSGSLGLPVVGETLRFIASAYSPRP 60
           MDS W FVQLITAAAFI S+ I+I K   SS PSLP+GSLGL  VGETL F++SAYS RP
Sbjct: 1   MDSLWIFVQLITAAAFICSTIIVILKASSSSKPSLPAGSLGLLFVGETLHFVSSAYSHRP 60

Query: 61  ETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFVPSYPKSLTELMGKSS 120
           ++FMD RRRRYG VFKSH+FGSPTIVSTDAEV+RF+LQSDAKAFV SYPKS+T+LMGK+S
Sbjct: 61  DSFMDTRRRRYGKVFKSHLFGSPTIVSTDAEVNRFVLQSDAKAFVTSYPKSVTDLMGKAS 120

Query: 121 ILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWSSSHHNPIHIQDEAKN 180
           ILLING  HR+IHGLIGAFFKS HLKAQLTID+QK+LQ S+S WSSS HNPIHIQDEAKN
Sbjct: 121 ILLINGPLHRRIHGLIGAFFKSPHLKAQLTIDMQKFLQKSMSTWSSSQHNPIHIQDEAKN 180

Query: 181 AKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLNDGSQQVTDELIAENMIDMMIP 240
           AKRKMV  V+E+I+ +RKKM ENL N  +P DVVD+LLNDGS QVTDELIA+N+IDMMIP
Sbjct: 181 AKRKMVKLVEEIIEGKRKKMNENLSNNRVPNDVVDILLNDGSHQVTDELIADNIIDMMIP 240

Query: 241 GHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVIS 300
           G DSVPILITLAIKYLSDCP  LQ L EENMELK+LKTQLGEPLKW DYLSLPFTQNVI+
Sbjct: 241 GQDSVPILITLAIKYLSDCPLALQRLVEENMELKRLKTQLGEPLKWTDYLSLPFTQNVIT 300

Query: 301 ETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQN 360
           ETLRMGNIITGVMRKA+KD+EIKG+LIPKGWCVL+YFRSVH+DE+HFESPY+FNPWRWQ+
Sbjct: 301 ETLRMGNIITGVMRKAVKDVEIKGYLIPKGWCVLVYFRSVHVDEEHFESPYHFNPWRWQD 360

Query: 361 KDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEEDKIINFPTVRMKKGM 420
           KDSNS NFTPFGGG RLCPGL+LARLE SIFLHHFVTEFRWVAEED IINFPTVRMKK M
Sbjct: 361 KDSNSLNFTPFGGGQRLCPGLELARLETSIFLHHFVTEFRWVAEEDNIINFPTVRMKKRM 420

Query: 421 AVW 424
           A+W
Sbjct: 421 AIW 423

[Close]

BLAST of Lag0016800 vs. NCBI nr
Match: XP_022956333.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita moschata])

HSP 1 Score: 688.7 bits (1776), Expect = 3.2e-194
Identity = 348/479 (72.65%), Postives = 385/479 (80.38%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFISSPPSLPSGSLGLPVVGETLRFIASAYSPRP 60
           MDS W FVQLITAAAFI S  I+I K   SS PSLP+GSLGL  VGETL F++SAYS RP
Sbjct: 1   MDSLWIFVQLITAAAFICSIIIVILKASSSSKPSLPAGSLGLLFVGETLHFVSSAYSHRP 60

Query: 61  ETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFVPSYPKSLTELMGKSS 120
           ++FMD RR RYG VFKSH+FGSPTIVSTDAEV+RF+LQSDAKAFV SYPKS+T+LMGK+S
Sbjct: 61  DSFMDTRRLRYGKVFKSHLFGSPTIVSTDAEVNRFVLQSDAKAFVTSYPKSVTDLMGKAS 120

Query: 121 ILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWSSSHHNPIHIQDEAKN 180
           ILLING  HR+IHGLIGAFFKS+HLKAQLTID+QK+LQ S+S WSSS HNPIHIQDEAKN
Sbjct: 121 ILLINGPLHRRIHGLIGAFFKSAHLKAQLTIDMQKFLQKSMSTWSSSQHNPIHIQDEAKN 180

Query: 181 --------------------------------------------------AKRKMVGFVQ 240
                                                             AKRKMV  V+
Sbjct: 181 IAFEVLVKTLMSLEGGEEMEFLKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVE 240

Query: 241 EMIQSRRKKMKENLKNIVLPKDVVDVLLNDGSQQVTDELIAENMIDMMIPGHDSVPILIT 300
           E+I+ +RKKM ENL N  +P DVVD+LLNDGS QVTDELIA+N+IDMMIPG DSVPILIT
Sbjct: 241 EIIEGKRKKMNENLSNNRVPNDVVDILLNDGSHQVTDELIADNIIDMMIPGQDSVPILIT 300

Query: 301 LAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVISETLRMGNIIT 360
           LAIKYLSDCP  LQ L EENMELK+LKTQLGEPLKW DYLSLPFTQNVI+ETLRMGNIIT
Sbjct: 301 LAIKYLSDCPLALQRLVEENMELKRLKTQLGEPLKWTDYLSLPFTQNVITETLRMGNIIT 360

Query: 361 GVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQNKDSNSSNFTP 420
           GVMRKA+KD+EIKG+LIPKGWCVL+YFRSVH+DE+HFESPY+FNPWRWQ+KDS S NFTP
Sbjct: 361 GVMRKAVKDVEIKGYLIPKGWCVLVYFRSVHVDEEHFESPYHFNPWRWQDKDSTSLNFTP 420

Query: 421 FGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEEDKIINFPTVRMKKGMAVWVKKRED 430
           FGGG RLCPGL+LARLE SIFLHHFVTEFRWVAEED IINFPTVRMKK MA+WVKKRED
Sbjct: 421 FGGGQRLCPGLELARLETSIFLHHFVTEFRWVAEEDNIINFPTVRMKKRMAIWVKKRED 479

[Close]

BLAST of Lag0016800 vs. NCBI nr
Match: XP_038905609.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1-like [Benincasa hispida])

HSP 1 Score: 672.2 bits (1733), Expect = 3.1e-189
Identity = 353/498 (70.88%), Postives = 386/498 (77.51%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFISSP-------------------PSLPSGSLG 60
           MDS W FVQLITAAAF +SSTIII K+FI+ P                   PSLPSGSLG
Sbjct: 1   MDSLWIFVQLITAAAF-MSSTIIILKSFINIPTTPSSSSSSSSNSFISSNNPSLPSGSLG 60

Query: 61  LPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDA 120
           LP VGETL FI+SAYS RP+TFMD RRRRYG VFKSHIFGSPTIVSTDAEV+RFILQSDA
Sbjct: 61  LPFVGETLHFISSAYSQRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDAEVNRFILQSDA 120

Query: 121 KAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSI 180
           K FVPSYPKS+TELMGKSSILLING+ HR+IHGLIGAFFKSSHLKAQ+TID+QKYL+ SI
Sbjct: 121 KFFVPSYPKSVTELMGKSSILLINGSLHRRIHGLIGAFFKSSHLKAQVTIDMQKYLKKSI 180

Query: 181 SIWSSS-HHNPIHIQDEAKN---------------------------------------- 240
             W+++  HNPI+IQDEAKN                                        
Sbjct: 181 PTWTNTCQHNPIYIQDEAKNIAFEVLVKTLISLEAGDEMEFLKKQFNQFIAGIMAFPINI 240

Query: 241 ----------AKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLNDGSQQVTDELI 300
                     AKRKMV  VQE+I+ RRKK  E L N +   DVVDVL+NDGSQQVTDELI
Sbjct: 241 PGSRLYRSLQAKRKMVKLVQEIIEGRRKKKGEKLSNAIPINDVVDVLVNDGSQQVTDELI 300

Query: 301 AENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYL 360
           A+NMIDMMIPG DSVPILITLAIKYLSD PS L  L EENMELK+LKTQLGE LKW+DYL
Sbjct: 301 ADNMIDMMIPGQDSVPILITLAIKYLSDSPSALHQLVEENMELKRLKTQLGETLKWSDYL 360

Query: 361 SLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESP 420
           SLPFTQNVI+ETLR+GNIITGVMRK MKD+EIKG+LIPKGWCVL Y RSVH+DE HFESP
Sbjct: 361 SLPFTQNVITETLRIGNIITGVMRKTMKDVEIKGYLIPKGWCVLAYIRSVHVDENHFESP 420

Query: 421 YNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEEDKIIN 429
           Y+FNPWRWQ+KDSN+ NFTPFGGG RLCPGL+LARLEASIFLHHFVTEFRWVAEED IIN
Sbjct: 421 YHFNPWRWQDKDSNNFNFTPFGGGQRLCPGLELARLEASIFLHHFVTEFRWVAEEDNIIN 480

[Close]

BLAST of Lag0016800 vs. NCBI nr
Match: XP_008447603.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Cucumis melo])

HSP 1 Score: 671.0 bits (1730), Expect = 6.8e-189
Identity = 354/502 (70.52%), Postives = 385/502 (76.69%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFI---------------SSPPSLPSGSLGLPVV 60
           MDS W FVQ+ITAAAF ISSTIII K FI               S+ PSLPSGSLGLP V
Sbjct: 1   MDSLWIFVQVITAAAF-ISSTIIILKNFIIPTTPSSSSSNSFTSSNNPSLPSGSLGLPFV 60

Query: 61  GETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFV 120
           GETL F++SAYS RP+TFMD RRRRYG VFKSHIFGSPTIVSTD EV+RFILQSD+K FV
Sbjct: 61  GETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFV 120

Query: 121 PSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWS 180
           PSYPKS+TELMGKSSILLINGT HR+IHGLIGAFFKSSHLKAQ+T+D+QKYLQ SIS W+
Sbjct: 121 PSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLQKSISTWT 180

Query: 181 SS-HHNPIHIQDEAKN-------------------------------------------- 240
           S+  HNPIHIQDEAKN                                            
Sbjct: 181 STCQHNPIHIQDEAKNIAFEVLVKTLVSLESGEEMEFLKKQFNQFIAGIMAFPINIPGTT 240

Query: 241 ------AKRKMVGFVQEMIQSRRKKMKE--------NLKNIVLPKDVVDVLLNDGSQQVT 300
                 AKRKMV  V+E+I+ RRKK K+        N   + +  DVVDVLLNDGSQQVT
Sbjct: 241 LYRSLQAKRKMVKLVEEIIEGRRKKKKKKSEKYWSNNNNGVAIINDVVDVLLNDGSQQVT 300

Query: 301 DELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKW 360
           DE+IA NMIDMMIPG DSVPILITLAIKYLSD PS LQ LTEENMELK+ KTQLGEPLKW
Sbjct: 301 DEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKRQKTQLGEPLKW 360

Query: 361 NDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKH 420
            DYLSLPFTQNVI+ETLR+GNIITGVMRKAMKDIEIKG+LIPKGWCVL Y RSVH+DE  
Sbjct: 361 TDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLAYIRSVHVDENQ 420

Query: 421 FESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEED 429
           FESPY+FNPWRWQ KDSN+ NFTPFGGG RLCPGL+LARLEASIFLHHF+TEFRWVAEED
Sbjct: 421 FESPYHFNPWRWQGKDSNNLNFTPFGGGQRLCPGLELARLEASIFLHHFITEFRWVAEED 480

[Close]

BLAST of Lag0016800 vs. NCBI nr
Match: XP_011652876.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus] >KAE8651292.1 hypothetical protein Csa_002432 [Cucumis sativus])

HSP 1 Score: 663.3 bits (1710), Expect = 1.4e-186
Identity = 351/505 (69.50%), Postives = 383/505 (75.84%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFI---------------SSPPSLPSGSLGLPVV 60
           MDS W FVQLITAAAF ISSTIII + FI               S+ PSLPSGSLGLP V
Sbjct: 1   MDSLWIFVQLITAAAF-ISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFV 60

Query: 61  GETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFV 120
           GETL F++SAYS RP+TFMD RRRRYG VFKSHIFGSPTIVSTD EV+RFILQSD+K FV
Sbjct: 61  GETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFV 120

Query: 121 PSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWS 180
           PSYPKS+TELMGKSSILLINGT HR+IHGLIGAFFKSSHLKAQ+T+D+QKYL  SIS W+
Sbjct: 121 PSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWT 180

Query: 181 SS-HHNPIHIQDEAKN-------------------------------------------- 240
           S+   NPIHIQDEAKN                                            
Sbjct: 181 STCQQNPIHIQDEAKNIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAFPINIPGTT 240

Query: 241 ------AKRKMVGFVQEMIQSRRKKMK-----------ENLKNIVLPKDVVDVLLNDGSQ 300
                 AKRKMV  V+E+I+ RRKK K            N   + +  DVVDVLLNDGSQ
Sbjct: 241 LYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVDVLLNDGSQ 300

Query: 301 QVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEP 360
           QVTDE+IA NMIDMMIPG DSVPILITLAIKYLSD PS LQ LTEENMELK+ KTQ GEP
Sbjct: 301 QVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKRQKTQFGEP 360

Query: 361 LKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLD 420
           LKW+DYLSLPFTQNVI+ETLR+GNIITGVMRKAMKDIEIKG+LIPKGWCVL Y RSVH+D
Sbjct: 361 LKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLAYIRSVHVD 420

Query: 421 EKHFESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVA 429
           E HFESPY+FNPWRWQ KDSN+ NF+PFGGG RLCPGL+LARLEASIFLHHFVTEFRWVA
Sbjct: 421 ENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHFVTEFRWVA 480

[Close]

BLAST of Lag0016800 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.1e-146
Identity = 259/447 (57.94%), Postives = 316/447 (70.69%), Query Frame = 0


Query: 30  SSPPSLPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTD 89
           S  P  P GSLG PV+GET+ F++SAYS RPE+FMD RR  YG VFKSHIFG+ TIVSTD
Sbjct: 46  SHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTD 105

Query: 90  AEVSRFILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQL 149
           AEV+R +LQSD+ AFVP YPK++ ELMGKSSILLING+ HR+ HGL+G+F KS  LKAQ+
Sbjct: 106 AEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQI 165

Query: 150 TIDLQKYLQNSISIWSSSHHNPIHIQDEAK------------------------------ 209
             D+ K+L  S+ +WS     P+ +QD +K                              
Sbjct: 166 VRDMHKFLSESMDLWSED--QPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENF 225

Query: 210 --------------------NAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLN 269
                                AK+ MV  V+ +I+ + +K K   ++ V+ KDVVDVLL 
Sbjct: 226 ISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLK 285

Query: 270 DGSQQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQ 329
           D S+ +T  LIA NMIDMMIPGHDSVP+LITLA+K+LSD P+ L  LTEENM+LK LK  
Sbjct: 286 DSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKEL 345

Query: 330 LGEPLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRS 389
            GEPL WNDYLSLPFTQ VI+ETLRMGN+I GVMRKAMKD+EIKG++IPKGWC L Y RS
Sbjct: 346 TGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRS 405

Query: 390 VHLDEKHFESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEF 427
           VHLD+ ++ESPY FNPWRWQ +D N+S+F+PFGGG RLCPGLDLARLE S+FLHH VT F
Sbjct: 406 VHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRF 465

[Close]

BLAST of Lag0016800 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.0e-142
Identity = 249/447 (55.70%), Postives = 322/447 (72.04%), Query Frame = 0


Query: 35  LPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGN-VFKSHIFGSPTIVSTDAEVS 94
           LP GS G PVVGETL F++ AYSPRPE F+D RR+ +G+ VF+SH+FGS T+V+ DAEVS
Sbjct: 49  LPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEVS 108

Query: 95  RFILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDL 154
           RF+LQSDA+AFVP YP+SLTELMGKSSILLING   R++HGL+GAFFKSSHLK+QLT D+
Sbjct: 109 RFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTADM 168

Query: 155 QKYLQNSISIWSSSHHNPIHIQDEAKN--------------------------------- 214
           ++ L  ++S +  S  + +H+Q  AK+                                 
Sbjct: 169 RRRLSPALSSFPDS--SLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGL 228

Query: 215 -----------------AKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLNDGSQ 274
                            AK+KM   +Q +I+ +R +          P+D +DVL+ DGS 
Sbjct: 229 MSLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASP----PRDAIDVLIGDGSD 288

Query: 275 QVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEP 334
           ++TDELI++NMID+MIP  DSVP+LITLA+K+LS+CP  L  L EEN++LK+ KT +GE 
Sbjct: 289 ELTDELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGET 348

Query: 335 LKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLD 394
           L+W DY+SL FTQ+VI+ETLR+GNII G+MRKA++D+E+KGHLIPKGWCV +YFRSVHLD
Sbjct: 349 LQWTDYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLD 408

Query: 395 EKHFESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVA 431
           +  ++ PY FNPWRW+ KD ++ +FTPFGGG RLCPGLDLARLEASIFLHH VT FRWVA
Sbjct: 409 DTLYDEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWVA 468

[Close]

BLAST of Lag0016800 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 407.1 bits (1045), Expect = 2.4e-112
Identity = 211/454 (46.48%), Postives = 292/454 (64.32%), Query Frame = 0


Query: 35  LPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSR 94
           +P+GSLG PV+GETL FIA  YS RP TFMD R+  YG VFK++I G+P I+STDAEV++
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 95  FILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQ 154
            +LQ+    FVP+YPKS+TEL+G++SIL ING   +++H LIGAF +S HLK ++T D++
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 155 KYLQNSISIWSSSHHNPIHIQDEAK----------------------------------- 214
             +  +++ W+      +H+QDE K                                   
Sbjct: 189 ASVVLTLASWAQL--PLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLI 248

Query: 215 ---------------NAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLNDG--- 274
                           AK +++  V+++++ R+  M           DVVDVLL DG   
Sbjct: 249 CIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSP----ANDVVDVLLRDGGDS 308

Query: 275 -SQQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQL 334
             Q    + ++  +++MMIPG +++P  +TLA+K+LSD P  L  L EENME+K+ K +L
Sbjct: 309 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLEL 368

Query: 335 GEPLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSV 394
           GE  KW DY+SL FTQNVI+ETLRM NII GV RKA+KD+EIKG+LIPKGWCVL  F SV
Sbjct: 369 GEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISV 428

Query: 395 HLDEKHFESPYNFNPWRWQ--NKDSNSS-NFTPFGGGLRLCPGLDLARLEASIFLHHFVT 432
           H+DE  +++PY F+PWRW   N  +NSS  FTPFGGG RLCPGL+L++LE SIFLHH VT
Sbjct: 429 HMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVT 488

[Close]

BLAST of Lag0016800 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 3.0e-86
Identity = 175/450 (38.89%), Postives = 266/450 (59.11%), Query Frame = 0


Query: 35  LPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSR 94
           LP GSLGLP++GET + I +  +  PE F+D R  RYG+VF +H+FG PTI S D E +R
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 95  FILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQ 154
           F+LQ++ K F  SYP S+  L+GK S+LL+ G+ H+++H L  +F  SS +K  L +D+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 155 KYLQNSISIWSSSHHNPIHIQDEAK----------------------------------- 214
           + ++ ++  WSS     + + +EAK                                   
Sbjct: 151 RLVRFNLDSWSSR----VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFF 210

Query: 215 ---------------NAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLL--NDGS 274
                           A+RK+   +  ++  RR++ +E  +     KD++  LL  +DG 
Sbjct: 211 SLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAER---KKDMLAALLAADDG- 270

Query: 275 QQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGE 334
              +DE I + ++ +++ G+++   ++TLA+K+L++ P  L  L EE+ +++ +K+    
Sbjct: 271 --FSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SY 330

Query: 335 PLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHL 394
            L+W+DY S+PFTQ V++ETLR+ NII GV R+AM D+EIKG+ IPKGW V   FR+VHL
Sbjct: 331 SLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHL 390

Query: 395 DEKHFESPYNFNPWRWQNKD---SNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEF 429
           D  HF+    FNPWRWQ+       S+ FTPFGGG RLCPG +LAR+  S+FLH  VT F
Sbjct: 391 DPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGF 450

[Close]

BLAST of Lag0016800 vs. ExPASy Swiss-Prot
Match: Q5CCK1 (Cytochrome P450 90A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A4 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.5e-74
Identity = 166/484 (34.30%), Postives = 264/484 (54.55%), Query Frame = 0


Query: 10  LITAAAFIISSTIIIFKTFISSPPS--------LPSGSLGLPVVGETLRFIASAYSPRPE 69
           L  AAA ++ +  + +   +  P +        +P GS GLP++GETLR I++  +P PE
Sbjct: 9   LAAAAAAVVVAMALRWLLLLGGPAAGRLGKRARMPPGSTGLPLIGETLRLISAYKTPNPE 68

Query: 70  TFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFVPSYPKSLTELMGKSSI 129
            F+D R  R+G VF +H+FG  T+ S D   +R +L ++ +A   SYP S+  L+G  S+
Sbjct: 69  PFIDERVARHGGVFTTHVFGERTVFSADPAFNRLLLAAEGRAVHSSYPSSIATLLGARSL 128

Query: 130 LLINGTFHRKIHGL---------------------------------IGAFFKSSHLKAQ 189
           LL  G  H+++H L                                 +    ++  +   
Sbjct: 129 LLTRGAAHKRLHSLTLTRLGRPASPPLLAHIDRLVLATMRQWEPAATVRLMDEAKKITFN 188

Query: 190 LTI-----------------DLQKYLQNSISI-WSSSHHNPIHIQDEAKNAKRKMVGFVQ 249
           LT+                 +  K +    SI +  ++  P     +A  A++K+ G ++
Sbjct: 189 LTVKQLVSIEPGPWTESLRREYVKLIDGFFSIPFPLAYFLPFTTYGQALKARKKVAGALR 248

Query: 250 EMIQSRRKKMKENLKNI------VLPKDVVDVLLNDGSQQVTDELIAENMIDMMIPGHDS 309
           E+I+ R ++  EN  +I         KD+V+ LL       ++E + +  + +++ G+++
Sbjct: 249 EVIKKRMEEKAENGGSIGDDEGKKEKKDMVEELLQAEGGSFSEEEMVDFCLSLLVAGYET 308

Query: 310 VPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVISETLR 369
             +L+TLA+K+L++ P+ L  L EE+  ++ +K +  +PL+W+DY S+PFTQ VI+ETLR
Sbjct: 309 TSVLMTLAVKFLTETPAALAELKEEHANIRDMKGK-NQPLEWSDYKSMPFTQCVINETLR 368

Query: 370 MGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQ--NKD 423
           +GNII+GV R+A  DI  K + IPKG  +   FR+VHL+ +H+E+   FNPWRWQ  NK 
Sbjct: 369 VGNIISGVFRRANTDIHYKDYTIPKGCKIFASFRAVHLNNEHYENARTFNPWRWQINNKL 428

[Close]

BLAST of Lag0016800 vs. ExPASy TrEMBL
Match: A0A6J1GWI2 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111458064 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.5e-194
Identity = 348/479 (72.65%), Postives = 385/479 (80.38%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFISSPPSLPSGSLGLPVVGETLRFIASAYSPRP 60
           MDS W FVQLITAAAFI S  I+I K   SS PSLP+GSLGL  VGETL F++SAYS RP
Sbjct: 1   MDSLWIFVQLITAAAFICSIIIVILKASSSSKPSLPAGSLGLLFVGETLHFVSSAYSHRP 60

Query: 61  ETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFVPSYPKSLTELMGKSS 120
           ++FMD RR RYG VFKSH+FGSPTIVSTDAEV+RF+LQSDAKAFV SYPKS+T+LMGK+S
Sbjct: 61  DSFMDTRRLRYGKVFKSHLFGSPTIVSTDAEVNRFVLQSDAKAFVTSYPKSVTDLMGKAS 120

Query: 121 ILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWSSSHHNPIHIQDEAKN 180
           ILLING  HR+IHGLIGAFFKS+HLKAQLTID+QK+LQ S+S WSSS HNPIHIQDEAKN
Sbjct: 121 ILLINGPLHRRIHGLIGAFFKSAHLKAQLTIDMQKFLQKSMSTWSSSQHNPIHIQDEAKN 180

Query: 181 --------------------------------------------------AKRKMVGFVQ 240
                                                             AKRKMV  V+
Sbjct: 181 IAFEVLVKTLMSLEGGEEMEFLKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVE 240

Query: 241 EMIQSRRKKMKENLKNIVLPKDVVDVLLNDGSQQVTDELIAENMIDMMIPGHDSVPILIT 300
           E+I+ +RKKM ENL N  +P DVVD+LLNDGS QVTDELIA+N+IDMMIPG DSVPILIT
Sbjct: 241 EIIEGKRKKMNENLSNNRVPNDVVDILLNDGSHQVTDELIADNIIDMMIPGQDSVPILIT 300

Query: 301 LAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVISETLRMGNIIT 360
           LAIKYLSDCP  LQ L EENMELK+LKTQLGEPLKW DYLSLPFTQNVI+ETLRMGNIIT
Sbjct: 301 LAIKYLSDCPLALQRLVEENMELKRLKTQLGEPLKWTDYLSLPFTQNVITETLRMGNIIT 360

Query: 361 GVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQNKDSNSSNFTP 420
           GVMRKA+KD+EIKG+LIPKGWCVL+YFRSVH+DE+HFESPY+FNPWRWQ+KDS S NFTP
Sbjct: 361 GVMRKAVKDVEIKGYLIPKGWCVLVYFRSVHVDEEHFESPYHFNPWRWQDKDSTSLNFTP 420

Query: 421 FGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEEDKIINFPTVRMKKGMAVWVKKRED 430
           FGGG RLCPGL+LARLE SIFLHHFVTEFRWVAEED IINFPTVRMKK MA+WVKKRED
Sbjct: 421 FGGGQRLCPGLELARLETSIFLHHFVTEFRWVAEEDNIINFPTVRMKKRMAIWVKKRED 479

[Close]

BLAST of Lag0016800 vs. ExPASy TrEMBL
Match: A0A1S3BHU7 (3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103490016 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 3.3e-189
Identity = 354/502 (70.52%), Postives = 385/502 (76.69%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFI---------------SSPPSLPSGSLGLPVV 60
           MDS W FVQ+ITAAAF ISSTIII K FI               S+ PSLPSGSLGLP V
Sbjct: 1   MDSLWIFVQVITAAAF-ISSTIIILKNFIIPTTPSSSSSNSFTSSNNPSLPSGSLGLPFV 60

Query: 61  GETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFV 120
           GETL F++SAYS RP+TFMD RRRRYG VFKSHIFGSPTIVSTD EV+RFILQSD+K FV
Sbjct: 61  GETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFV 120

Query: 121 PSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWS 180
           PSYPKS+TELMGKSSILLINGT HR+IHGLIGAFFKSSHLKAQ+T+D+QKYLQ SIS W+
Sbjct: 121 PSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLQKSISTWT 180

Query: 181 SS-HHNPIHIQDEAKN-------------------------------------------- 240
           S+  HNPIHIQDEAKN                                            
Sbjct: 181 STCQHNPIHIQDEAKNIAFEVLVKTLVSLESGEEMEFLKKQFNQFIAGIMAFPINIPGTT 240

Query: 241 ------AKRKMVGFVQEMIQSRRKKMKE--------NLKNIVLPKDVVDVLLNDGSQQVT 300
                 AKRKMV  V+E+I+ RRKK K+        N   + +  DVVDVLLNDGSQQVT
Sbjct: 241 LYRSLQAKRKMVKLVEEIIEGRRKKKKKKSEKYWSNNNNGVAIINDVVDVLLNDGSQQVT 300

Query: 301 DELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKW 360
           DE+IA NMIDMMIPG DSVPILITLAIKYLSD PS LQ LTEENMELK+ KTQLGEPLKW
Sbjct: 301 DEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKRQKTQLGEPLKW 360

Query: 361 NDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKH 420
            DYLSLPFTQNVI+ETLR+GNIITGVMRKAMKDIEIKG+LIPKGWCVL Y RSVH+DE  
Sbjct: 361 TDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLAYIRSVHVDENQ 420

Query: 421 FESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEED 429
           FESPY+FNPWRWQ KDSN+ NFTPFGGG RLCPGL+LARLEASIFLHHF+TEFRWVAEED
Sbjct: 421 FESPYHFNPWRWQGKDSNNLNFTPFGGGQRLCPGLELARLEASIFLHHFITEFRWVAEED 480

[Close]

BLAST of Lag0016800 vs. ExPASy TrEMBL
Match: A0A6J1D2F1 (3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Momordica charantia OX=3673 GN=LOC111016959 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.3e-185
Identity = 349/491 (71.08%), Postives = 389/491 (79.23%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIF-KTF------ISSPPSLPSGSLGLPVVGETLRFIA 60
           MDS   FVQLITAAA I S+ IIIF  TF       +S PSLPSGSLGLP +GETL+F++
Sbjct: 1   MDSLLIFVQLITAAALISSTLIIIFLLTFKPNSPASNSIPSLPSGSLGLPFIGETLQFVS 60

Query: 61  SAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFVPSYPKSLT 120
           SAYS RPE+FMD RR  YG VFKSHIFGSPTIVSTD EVSRFILQSDA+ FVP YPKSLT
Sbjct: 61  SAYSHRPESFMDRRRCWYGKVFKSHIFGSPTIVSTDGEVSRFILQSDARTFVPCYPKSLT 120

Query: 121 ELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWSSS-HHNPI 180
           ELMGKSSILLING+ HR+IHGLIGAFFKSSHLKAQLT  + KYLQNSISIW+++  HNPI
Sbjct: 121 ELMGKSSILLINGSLHRRIHGLIGAFFKSSHLKAQLTKHMHKYLQNSISIWTTTCQHNPI 180

Query: 181 HIQDEAKN--------------------------------------------------AK 240
           HIQDEAK+                                                  AK
Sbjct: 181 HIQDEAKSIAFEVLVKTLISLDPGEEMEFLKKQFKEFIAGLMALPINIPGSRLYTSLQAK 240

Query: 241 RKMVGFVQEMIQSRRK-KMKENL-KNIVLPKDVVDVLLNDGSQQVTDELIAENMIDMMIP 300
           RKMV FV+E+IQ+RRK KM E+L  N  +PKDVVDVLLNDGS ++TD+LIAEN+IDMMIP
Sbjct: 241 RKMVKFVEEIIQARRKQKMSESLNNNNKVPKDVVDVLLNDGSHEITDDLIAENVIDMMIP 300

Query: 301 GHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVIS 360
           G DSVPIL+TLAIKYLSDCP+ LQ L EENMELK+ KTQLGE  KWNDYLSLPFTQNVI 
Sbjct: 301 GQDSVPILMTLAIKYLSDCPAALQQLREENMELKRQKTQLGESSKWNDYLSLPFTQNVIM 360

Query: 361 ETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQN 420
           ETLRMGNIITGVMRKAMKDIEIKG+LIPKGWC+L+YFRSVHLDE HFESPY+FNPWRWQ+
Sbjct: 361 ETLRMGNIITGVMRKAMKDIEIKGYLIPKGWCILLYFRSVHLDENHFESPYHFNPWRWQD 420

Query: 421 KDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEFRWVAEEDKIINFPTVRMKKGM 432
           KDSN+ NFTPFGGGLRLCPGL+LARLEASIFLHHFVTEFRWVAE+D +I+FPTVRMKK M
Sbjct: 421 KDSNNPNFTPFGGGLRLCPGLELARLEASIFLHHFVTEFRWVAEKDTVISFPTVRMKKRM 480

[Close]

BLAST of Lag0016800 vs. ExPASy TrEMBL
Match: A0A6J1IZU1 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479936 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.9e-177
Identity = 319/446 (71.52%), Postives = 358/446 (80.27%), Query Frame = 0


Query: 5   WFFVQLITAAAFIISSTIIIFKTFISSPPSLPSGSLGLPVVGETLRFIASAYSPRPETFM 64
           W FVQLITAAAFI S+ I+I K   SS PSLPSGSLGL  VGETL F++SAYS RP++FM
Sbjct: 2   WIFVQLITAAAFICSTIIVILKASSSSKPSLPSGSLGLVFVGETLHFVSSAYSHRPDSFM 61

Query: 65  DIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFVPSYPKSLTELMGKSSILLI 124
           D RRRRYG VFKSH+FGSPTIVSTDAEV+RF+LQSDAKAFV SYPKS+T+LMGK+SILLI
Sbjct: 62  DTRRRRYGKVFKSHLFGSPTIVSTDAEVNRFVLQSDAKAFVTSYPKSVTDLMGKASILLI 121

Query: 125 NGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWSSSHHNPIHIQDEAKN---- 184
           NG  HR+IHGLIGAFFKS+HLKAQLTID+QK+LQ S+S WSSS HNPIHIQDEAKN    
Sbjct: 122 NGPLHRRIHGLIGAFFKSAHLKAQLTIDMQKFLQKSMSTWSSSQHNPIHIQDEAKNIAFE 181

Query: 185 ----------------------------------------------AKRKMVGFVQEMIQ 244
                                                         AKRKMV  V+E+I+
Sbjct: 182 LLVKTLMSLEGGEEMEFLKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIE 241

Query: 245 SRRKKMKENLKNIVLPKDVVDVLLNDGSQQVTDELIAENMIDMMIPGHDSVPILITLAIK 304
            +RKKM+EN+    +P DVVD+LLNDGS QVTDELIA+N+IDMMIPG DSVPILITLAIK
Sbjct: 242 GKRKKMRENMNINRVPNDVVDILLNDGSHQVTDELIADNIIDMMIPGQDSVPILITLAIK 301

Query: 305 YLSDCPSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVISETLRMGNIITGVMR 364
           +LSDCP  LQ L EENMELK+LKTQLGEPLKW DYLSLPFTQNVI+ETLRMGNIITGVMR
Sbjct: 302 FLSDCPLALQRLVEENMELKRLKTQLGEPLKWTDYLSLPFTQNVITETLRMGNIITGVMR 361

Query: 365 KAMKDIEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQNKDSNSSNFTPFGGG 401
           KA+KD+EIKG+LIPKGWCVL+YFRSVH+DE+HFESPY+FNPWRWQ+KDSNS NFTPFGGG
Sbjct: 362 KAVKDVEIKGYLIPKGWCVLVYFRSVHVDEEHFESPYHFNPWRWQDKDSNSLNFTPFGGG 421

[Close]

BLAST of Lag0016800 vs. ExPASy TrEMBL
Match: A0A5A7U5K3 (3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold511G00920 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 5.3e-171
Identity = 319/441 (72.34%), Postives = 350/441 (79.37%), Query Frame = 0


Query: 1   MDSFWFFVQLITAAAFIISSTIIIFKTFI---------------SSPPSLPSGSLGLPVV 60
           MDS W FVQ+ITAAAF ISSTIII K FI               S+ PSLPSGSLGLP V
Sbjct: 1   MDSLWIFVQVITAAAF-ISSTIIILKNFIIPTTPSSSSSNSFTSSNNPSLPSGSLGLPFV 60

Query: 61  GETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSRFILQSDAKAFV 120
           GETL F++SAYS RP+TFMD RRRRYG VFKSHIFGSPTIVSTD EV+RFILQSD+K FV
Sbjct: 61  GETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFV 120

Query: 121 PSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQKYLQNSISIWS 180
           PSYPKS+TELMGKSSILLINGT HR+IHGLIGAFFKSSHLKAQ+T+D+QKYLQ SIS W+
Sbjct: 121 PSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLQKSISTWT 180

Query: 181 SS-HHNPIHIQDEAKNA-------------------------KRKMVGFVQEMIQSRRKK 240
           S+  HNPIHIQDEAKN                           + + G +   I      
Sbjct: 181 STCQHNPIHIQDEAKNIAFEVLVKTLVSLESGEEMEFLKKQFNQFIAGIMAFPINIPGTT 240

Query: 241 MKENLKNIVLPKDVVDVLLNDGSQQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDC 300
           +  +L+ I +  DVVDVLLNDGSQQVTDE+IA NMIDMMIPG DSVPILITLAIKYLSD 
Sbjct: 241 LYRSLQAIAIINDVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDS 300

Query: 301 PSVLQHLTEENMELKKLKTQLGEPLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKD 360
           PS LQ LTEENMELK+ KTQLGEPLKW DYLSLPFTQNVI+ETLR+GNIITGVMRKAMKD
Sbjct: 301 PSALQQLTEENMELKRQKTQLGEPLKWTDYLSLPFTQNVITETLRLGNIITGVMRKAMKD 360

Query: 361 IEIKGHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCP 401
           IEIKG+LIPKGWCVL Y RSVH+DE HFESPY+FNPWRWQ KDSN+ NFTPFGGG RLCP
Sbjct: 361 IEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFTPFGGGQRLCP 420

[Close]

BLAST of Lag0016800 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 521.2 bits (1341), Expect = 8.1e-148
Identity = 259/447 (57.94%), Postives = 316/447 (70.69%), Query Frame = 0


Query: 30  SSPPSLPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTD 89
           S  P  P GSLG PV+GET+ F++SAYS RPE+FMD RR  YG VFKSHIFG+ TIVSTD
Sbjct: 46  SHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTD 105

Query: 90  AEVSRFILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQL 149
           AEV+R +LQSD+ AFVP YPK++ ELMGKSSILLING+ HR+ HGL+G+F KS  LKAQ+
Sbjct: 106 AEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQI 165

Query: 150 TIDLQKYLQNSISIWSSSHHNPIHIQDEAK------------------------------ 209
             D+ K+L  S+ +WS     P+ +QD +K                              
Sbjct: 166 VRDMHKFLSESMDLWSED--QPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENF 225

Query: 210 --------------------NAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLN 269
                                AK+ MV  V+ +I+ + +K K   ++ V+ KDVVDVLL 
Sbjct: 226 ISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLK 285

Query: 270 DGSQQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQ 329
           D S+ +T  LIA NMIDMMIPGHDSVP+LITLA+K+LSD P+ L  LTEENM+LK LK  
Sbjct: 286 DSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKEL 345

Query: 330 LGEPLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRS 389
            GEPL WNDYLSLPFTQ VI+ETLRMGN+I GVMRKAMKD+EIKG++IPKGWC L Y RS
Sbjct: 346 TGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRS 405

Query: 390 VHLDEKHFESPYNFNPWRWQNKDSNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEF 427
           VHLD+ ++ESPY FNPWRWQ +D N+S+F+PFGGG RLCPGLDLARLE S+FLHH VT F
Sbjct: 406 VHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRF 465

[Close]

BLAST of Lag0016800 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.7e-113
Identity = 211/454 (46.48%), Postives = 292/454 (64.32%), Query Frame = 0


Query: 35  LPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSR 94
           +P+GSLG PV+GETL FIA  YS RP TFMD R+  YG VFK++I G+P I+STDAEV++
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 95  FILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQ 154
            +LQ+    FVP+YPKS+TEL+G++SIL ING   +++H LIGAF +S HLK ++T D++
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 155 KYLQNSISIWSSSHHNPIHIQDEAK----------------------------------- 214
             +  +++ W+      +H+QDE K                                   
Sbjct: 189 ASVVLTLASWAQL--PLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLI 248

Query: 215 ---------------NAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLLNDG--- 274
                           AK +++  V+++++ R+  M           DVVDVLL DG   
Sbjct: 249 CIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSP----ANDVVDVLLRDGGDS 308

Query: 275 -SQQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQL 334
             Q    + ++  +++MMIPG +++P  +TLA+K+LSD P  L  L EENME+K+ K +L
Sbjct: 309 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLEL 368

Query: 335 GEPLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSV 394
           GE  KW DY+SL FTQNVI+ETLRM NII GV RKA+KD+EIKG+LIPKGWCVL  F SV
Sbjct: 369 GEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISV 428

Query: 395 HLDEKHFESPYNFNPWRWQ--NKDSNSS-NFTPFGGGLRLCPGLDLARLEASIFLHHFVT 432
           H+DE  +++PY F+PWRW   N  +NSS  FTPFGGG RLCPGL+L++LE SIFLHH VT
Sbjct: 429 HMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVT 488

[Close]

BLAST of Lag0016800 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 2.1e-87
Identity = 175/450 (38.89%), Postives = 266/450 (59.11%), Query Frame = 0


Query: 35  LPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSR 94
           LP GSLGLP++GET + I +  +  PE F+D R  RYG+VF +H+FG PTI S D E +R
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 95  FILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQ 154
           F+LQ++ K F  SYP S+  L+GK S+LL+ G+ H+++H L  +F  SS +K  L +D+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 155 KYLQNSISIWSSSHHNPIHIQDEAK----------------------------------- 214
           + ++ ++  WSS     + + +EAK                                   
Sbjct: 151 RLVRFNLDSWSSR----VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFF 210

Query: 215 ---------------NAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLL--NDGS 274
                           A+RK+   +  ++  RR++ +E  +     KD++  LL  +DG 
Sbjct: 211 SLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAER---KKDMLAALLAADDG- 270

Query: 275 QQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGE 334
              +DE I + ++ +++ G+++   ++TLA+K+L++ P  L  L EE+ +++ +K+    
Sbjct: 271 --FSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SY 330

Query: 335 PLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHL 394
            L+W+DY S+PFTQ V++ETLR+ NII GV R+AM D+EIKG+ IPKGW V   FR+VHL
Sbjct: 331 SLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHL 390

Query: 395 DEKHFESPYNFNPWRWQNKD---SNSSNFTPFGGGLRLCPGLDLARLEASIFLHHFVTEF 429
           D  HF+    FNPWRWQ+       S+ FTPFGGG RLCPG +LAR+  S+FLH  VT F
Sbjct: 391 DPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGF 450

[Close]

BLAST of Lag0016800 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.6e-66
Identity = 154/476 (32.35%), Postives = 250/476 (52.52%), Query Frame = 0


Query: 34  SLPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVS 93
           +LP G  G P +GET+ ++    +     FM     +YG +++S++FG PTIVS DA ++
Sbjct: 38  NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLN 97

Query: 94  RFILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDL 153
           RFILQ++ + F  SYP+S+  ++GK S+L++ G  HR +  +   F   + L+  L  D+
Sbjct: 98  RFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDV 157

Query: 154 QKYLQNSISIWSSSHHNPIHIQDEAK----------------------NAKRKMVGFVQ- 213
           +++    +  W    ++    QDEAK                        K++ V F++ 
Sbjct: 158 ERHTLFVLDSW--QQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKG 217

Query: 214 --------------EMIQSR-------RKKMKENLKNI---------VLPKDVVDVLLND 273
                         + +QSR        +KM+E   +I         V  +D  ++  +D
Sbjct: 218 VVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSD 277

Query: 274 --GSQQVTDELI----------AENMIDMMI----PGHDSVPILITLAIKYLSDCPSVLQ 333
               Q+  D+L+           E ++D+++     GH++  + I LAI +L  CP  ++
Sbjct: 278 HVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVE 337

Query: 334 HLTEENMELKKLKTQLGE-PLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIK 393
            L EE++E+ + K +LGE  L W+DY  + FTQ VI+ETLR+GN++  + RKA+KD+  K
Sbjct: 338 ELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYK 397

Query: 394 GHLIPKGWCVLIYFRSVHLDEKHFESPYNFNPWRWQNKDSNSS------------NFTPF 427
           G+ IP GW VL    +VHLD   ++ P  FNPWRWQ +++ +S            N+ PF
Sbjct: 398 GYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPF 457

[Close]

BLAST of Lag0016800 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.5e-66
Identity = 138/377 (36.60%), Postives = 216/377 (57.29%), Query Frame = 0


Query: 35  LPSGSLGLPVVGETLRFIASAYSPRPETFMDIRRRRYGNVFKSHIFGSPTIVSTDAEVSR 94
           LP GSLGLP++GET + I +  +  PE F+D R  RYG+VF +H+FG PTI S D E +R
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 95  FILQSDAKAFVPSYPKSLTELMGKSSILLINGTFHRKIHGLIGAFFKSSHLKAQLTIDLQ 154
           F+LQ++ K F  SYP S+  L+GK S+LL+ G+ H+++H L  +F  SS +K  L +D+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 155 KYLQNSISIWSSSHHNPIHIQDEAK----------------------------------- 214
           + ++ ++  WSS     + + +EAK                                   
Sbjct: 151 RLVRFNLDSWSSR----VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFF 210

Query: 215 ---------------NAKRKMVGFVQEMIQSRRKKMKENLKNIVLPKDVVDVLL--NDGS 274
                           A+RK+   +  ++  RR++ +E  +     KD++  LL  +DG 
Sbjct: 211 SLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAER---KKDMLAALLAADDG- 270

Query: 275 QQVTDELIAENMIDMMIPGHDSVPILITLAIKYLSDCPSVLQHLTEENMELKKLKTQLGE 334
              +DE I + ++ +++ G+++   ++TLA+K+L++ P  L  L EE+ +++ +K+    
Sbjct: 271 --FSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SY 330

Query: 335 PLKWNDYLSLPFTQNVISETLRMGNIITGVMRKAMKDIEIKGHLIPKGWCVLIYFRSVHL 360
            L+W+DY S+PFTQ V++ETLR+ NII GV R+AM D+EIKG+ IPKGW V   FR+VHL
Sbjct: 331 SLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHL 390

The following BLAST results are available for this feature:

BLAST of Lag0016800 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581856.1	1.5e-199	81.32	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrospe...	[more]
XP_022956333.1	3.2e-194	72.65	3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita moschata]	[more]
XP_038905609.1	3.1e-189	70.88	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1-like [Benincasa hispida]	[more]
XP_008447603.1	6.8e-189	70.52	PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Cucumis melo]	[more]
XP_011652876.1	1.4e-186	69.50	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus] >KAE8651292...	[more]

BLAST of Lag0016800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94IA6	1.1e-146	57.94	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37...	[more]
Q94IW5	5.0e-142	55.70	Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV...	[more]
Q9M066	2.4e-112	46.48	3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37...	[more]
Q42569	3.0e-86	38.89	Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1	[more]
Q5CCK1	1.5e-74	34.30	Cytochrome P450 90A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A4 PE=2 SV...	[more]

BLAST of Lag0016800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GWI2	1.5e-194	72.65	3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A1S3BHU7	3.3e-189	70.52	3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1D2F1	1.3e-185	71.08	3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Momordica charantia OX=3673 G...	[more]
A0A6J1IZU1	4.9e-177	71.52	3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5A7U5K3	5.3e-171	72.34	3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of Lag0016800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13730.1	8.1e-148	57.94	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]
AT4G36380.1	1.7e-113	46.48	Cytochrome P450 superfamily protein	[more]
AT5G05690.1	2.1e-87	38.89	Cytochrome P450 superfamily protein	[more]
AT3G50660.1	1.6e-66	32.35	Cytochrome P450 superfamily protein	[more]
AT5G05690.2	3.5e-66	36.60	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 368..378 score: 51.92 coord: 68..87 score: 27.17 coord: 296..314 score: 24.21 coord: 378..401 score: 32.04 coord: 336..360 score: 29.07
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 27..427 e-value: 3.3E-84 score: 285.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 30..427
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 153..408 e-value: 4.6E-49 score: 167.4
None	No IPR available	PANTHER	PTHR24286:SF30	3-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1	coord: 182..421
None	No IPR available	PANTHER	PTHR24286:SF30	3-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1	coord: 15..181
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 15..181
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 182..421
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 371..380

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0016800.1	Lag0016800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen