Lag0016776 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0016776 vs. NCBI nr
Match: XP_022138764.1 (protein SRC2-like [Momordica charantia])

HSP 1 Score: 238.8 bits (608), Expect = 5.5e-59
Identity = 167/297 (56.23%), Postives = 186/297 (62.63%), Query Frame = 0

Query: 1   MEST-TLEITVVSARDLPEINLVSKMVVYVVVSLAG-----QKFKTNADSTRGINPNWNF 60
           MEST +LEI VVSA+DLPEINLVSKM  YVVVSLAG     QKFKTNADS+ GINPNWNF
Sbjct: 1   MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSLAGGGNSDQKFKTNADSSGGINPNWNF 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPV 120
           PMKFS+NELAST     LVFT+RC R LGDRD                       VTYPV
Sbjct: 61  PMKFSVNELAST-----LVFTIRCFRTLGDRD-----------------------VTYPV 120

Query: 121 KKPSGKTQGVLSFEYKFGERVVAVGGSVEKPAAGYAARGIG------YPPPQPAAGY-PS 180
           +KPSGKT G+L+F+Y+      +     +KP A YA RGIG       PPP PAAGY P 
Sbjct: 121 RKPSGKTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPHPAAGYPPP 180

Query: 181 SYPPYN---APPPNPQPY---NYGYPHQPPPPPHHYGYSPPPVVVEKK--TSGMGKILGV 240
             PPYN   APPP PQPY   +YGYP  PPP     GY P   VV KK   SGMGKIL V
Sbjct: 181 PPPPYNTGYAPPP-PQPYGYPHYGYPAPPPP-----GYLPAAGVVAKKETKSGMGKILAV 240

Query: 241 GAALLGGVVCGALVGEMAADLGQASYSGGGTTHSTVAMSNSTLQSCAQSQNTLGQIG 277
           GA +LGG+ CGA++GE AADLGQ    G  + +  V  SN    S A SQNTLG IG
Sbjct: 241 GAGILGGLACGAMLGECAADLGQPPSRGSSSGNGGVHRSN--FSSMAYSQNTLGGIG 261

BLAST of Lag0016776 vs. NCBI nr
Match: XP_023902118.1 (protein SRC2-like [Quercus suber])

HSP 1 Score: 200.7 bits (509), Expect = 1.7e-47
Identity = 135/275 (49.09%), Postives = 167/275 (60.73%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG------QKFKTNADSTRGINPNWNF 60
           ME  TLE+ VVSA+DL ++NL SKM VYVVVSL+G      QK KTN     G +P+WNF
Sbjct: 1   MEYRTLELNVVSAKDLKDVNLFSKMDVYVVVSLSGDSHHHKQKAKTNVAKDSGTSPSWNF 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPV 120
           PMKF+I E A+ QNR  L F +RC R LGD+D+G V+VP+ ELL S  D+ SM+ V+Y V
Sbjct: 61  PMKFTIEEYAAQQNRLTLEFKLRCDRSLGDKDIGVVYVPIQELLNSTGDK-SMKFVSYQV 120

Query: 121 KKPSGKTQGVLSFEYKFGERV--VAVGGSV--EKPAAGY----------AARGIGYPPPQ 180
           +KPSGK QG L+F YKFGE+V   AV   V  ++P   Y          A  G  YPPP 
Sbjct: 121 RKPSGKPQGELNFSYKFGEKVSNAAVAPKVYGDEPVTAYPVAKPVVGPSAPYGGSYPPPP 180

Query: 181 PAAGYPSSYPPYNAPPPNPQPYNYGYPHQPPPPPHHYGYSP------PPVVVEKKTSGMG 240
           P  G    YPP   PPP  QPY  GY   PPPPP  YGY P      PP V +++     
Sbjct: 181 PPPGPGYGYPP--PPPPYQQPYG-GY---PPPPPQGYGYPPGPGYGYPPPVQQQQPQKKN 240

Query: 241 KI-LGVGAALLGGVVCGALVGEMAADLGQASYSGG 249
           K  +G+GA LLGG + G L+G+M +D+G   Y GG
Sbjct: 241 KFGMGLGAGLLGGALGGLLIGDMVSDMG--DYGGG 266

BLAST of Lag0016776 vs. NCBI nr
Match: XP_030974281.1 (protein SRC2-like [Quercus lobata])

HSP 1 Score: 200.7 bits (509), Expect = 1.7e-47
Identity = 135/275 (49.09%), Postives = 168/275 (61.09%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG------QKFKTNADSTRGINPNWNF 60
           ME  TLE+ VVSA+DL ++NL SKM VYVVVSL+G      QK KTN     G +P+WNF
Sbjct: 1   MEYRTLELNVVSAKDLKDVNLFSKMDVYVVVSLSGDSHHHKQKTKTNVAKDSGTSPSWNF 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPV 120
           PMKF+I E A+ QNR +L F +RC R LGD+D+G V+VP+ ELL S  D+ SM+ V+Y V
Sbjct: 61  PMKFTIEEYAAQQNRLSLEFKLRCDRSLGDKDIGVVYVPIQELLNSKGDK-SMKFVSYQV 120

Query: 121 KKPSGKTQGVLSFEYKFGERV--VAVGGSV--EKPAAGY----------AARGIGYPPPQ 180
           +KPSGK QG L+F YKFGE+V   AV   V  ++P   Y          A  G  YPPP 
Sbjct: 121 RKPSGKPQGELNFSYKFGEKVSNAAVAPKVYGDEPVTAYPVAKPVVGPSAPYGGSYPPPP 180

Query: 181 PAAGYPSSYPPYNAPPPNPQPYNYGYPHQPPPPPHHYGYSP------PPVVVEKKTSGMG 240
           P  G    YPP   PPP  QPY  GY   PPPPP  YGY P      PP V +++     
Sbjct: 181 PPPGPGYGYPP--PPPPYQQPYG-GY---PPPPPQGYGYPPGPGYGYPPPVQQQQPQKKN 240

Query: 241 KI-LGVGAALLGGVVCGALVGEMAADLGQASYSGG 249
           K  +G+GA LLGG + G L+G+M +D+G   Y GG
Sbjct: 241 KFGMGLGAGLLGGALGGLLIGDMVSDMG--DYGGG 266

BLAST of Lag0016776 vs. NCBI nr
Match: KAF3972284.1 (hypothetical protein CMV_004197 [Castanea mollissima])

HSP 1 Score: 199.9 bits (507), Expect = 2.9e-47
Identity = 130/271 (47.97%), Postives = 161/271 (59.41%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG------QKFKTNADSTRGINPNWNF 60
           ME  TLE+ VVSA+DL ++NL SKM VYVVVSL+G      QK KTN     G NP+WNF
Sbjct: 1   MEYRTLELNVVSAKDLKDVNLFSKMDVYVVVSLSGDSHHHKQKAKTNVAKDSGTNPSWNF 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPV 120
           PMKF+I E A+ QNR  L F +RC R LGD+D+G V+VP+ ELL +  D  SM+ V+Y V
Sbjct: 61  PMKFTIEEYAAQQNRLTLEFKLRCDRSLGDKDIGVVYVPIQELLNTTGDNKSMKFVSYQV 120

Query: 121 KKPSGKTQGVLSFEYKFGERV--VAVGGSV--EKPAAGYAARGIGYPPPQPAAGYPSSYP 180
           +KPSGK QG L+F YKFGE+V   AV   V   +P   Y    +  P   P+A Y  SYP
Sbjct: 121 RKPSGKPQGELNFSYKFGEKVSNAAVAPKVYGAEPVTAYP---VAQPVVGPSAPYAGSYP 180

Query: 181 PYNAP------PPNPQPYNYGYPHQPPPPPHHYGYSP------PPVVVEKKTSGMGKILG 240
           P   P      PP P PY   Y   PPPPP  YGY P      PP V ++        +G
Sbjct: 181 PPPPPGPGYGYPPPPPPYQQPYGGYPPPPPQGYGYPPGPGYGYPPPVQQQPQKKNKFGMG 240

Query: 241 VGAALLGGVVCGALVGEMAADLGQASYSGGG 250
           +GA LLGG + G L+G+M +D+G     GGG
Sbjct: 241 LGAGLLGGALGGLLIGDMVSDMGDC---GGG 265

BLAST of Lag0016776 vs. NCBI nr
Match: XP_041000897.1 (protein SRC2-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 199.1 bits (505), Expect = 4.9e-47
Identity = 135/277 (48.74%), Postives = 160/277 (57.76%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG--QKFKTNADSTRGINPNWNFPMKF 60
           ME  TLEI V SA+DL ++NL SKM VYVVVSL+G  QK KT  D   G +P+WNFP+KF
Sbjct: 1   MEYRTLEINVTSAKDLKDLNLFSKMDVYVVVSLSGDKQKAKTKVDREGGTSPSWNFPVKF 60

Query: 61  SINELASTQNREALVFTVRCSRGL-GDRDVGEVHVPVAELLRSAVDRNSMRLVTYPVKKP 120
           +I+E A+ QNR  L F +RC R L GD+DVG V+VPVAELL S  D  SM+ V+Y V KP
Sbjct: 61  TIDESAARQNRLNLEFKLRCDRSLGGDKDVGVVYVPVAELLNSTGDGKSMQFVSYQVTKP 120

Query: 121 SGKTQGVLSFEYKFGERVVAVG------GSVEKPAAGYAA-------------------- 180
           SGK QG+L+F YKF E+V A G          +P   Y A                    
Sbjct: 121 SGKPQGMLNFSYKFIEKVAAGGEPLVPEAKAHEPVTAYPAPVVGTSALPYDGLYPPAAAP 180

Query: 181 ------RGIGYPPPQPAAGYPSSYPPYNAPPPNPQPYNYGYPHQPPPPPHHYGYSPPPVV 240
                  GIGYPPP P  GYP   P Y  PPP P    YGYP  PPPPP  YGY P    
Sbjct: 181 HPPPPPYGIGYPPPPPGYGYPPPPPGYGYPPPPP---GYGYP--PPPPPPGYGYQPGVQQ 240

Query: 241 VEKKTS-GMGKILGVGAALLGGVVCGALVGEMAADLG 242
            +KK   GM    G+GA LLGG + G L+G+M +D G
Sbjct: 241 PQKKNKFGM----GLGAGLLGGALGGLLIGDMVSDAG 268

BLAST of Lag0016776 vs. ExPASy Swiss-Prot
Match: O04133 (Protein SRC2 OS=Glycine max OX=3847 GN=SRC2 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.2e-35
Identity = 113/289 (39.10%), Postives = 145/289 (50.17%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG-----QKFKTNADSTRGINPNWNFP 60
           ME  TLE+ ++SA+D+  +NL SKM VY  VSL+G     Q   T+     G NP WN+P
Sbjct: 3   MEYRTLELNIISAKDIKNVNLFSKMDVYAAVSLSGDPLHPQGATTHVHKDAGSNPTWNYP 62

Query: 61  MKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPVK 120
           +KFS+NE  + +NR +L   +   R LGD  +G VHVP+ ELL +  D +S R V+Y V 
Sbjct: 63  VKFSVNESLAKENRLSLEIKLISDRTLGDTVIGTVHVPLRELLDNPGDDSSFRQVSYQVM 122

Query: 121 KPSGKTQGVLSFEYKFGERVVAVGGSVEK------------PAAG--------------- 180
           K S K++G L+F YKFGE V A      K            P AG               
Sbjct: 123 KQSRKSKGSLNFSYKFGEHVPAPAAKTPKAAKAGQEPVMAYPPAGAGSSSMPYGTPHPPP 182

Query: 181 ---YAARGIGYPPPQPAAGYPSSYPPYNAPPPN----PQPYNYGYPHQPPPPPHHYGYSP 240
              YAA G GYPP Q   GYP     Y  PP      PQ   YGYP Q        GY  
Sbjct: 183 PQQYAATGYGYPPQQVHGGYPPQ-AAYGYPPQTGYGYPQQSGYGYPQQS-------GYGY 242

Query: 241 PPVVVEKKTSGMGKILGVGAALLGGVVCGALVGEMAADLGQ--ASYSGG 249
           PP   + K +  G  +G+GA LLGG + G L+G+M +D  +  A Y  G
Sbjct: 243 PPQAQKPKKNKFG--MGLGAGLLGGALGGMLIGDMVSDAAEYDAGYDAG 281

BLAST of Lag0016776 vs. ExPASy Swiss-Prot
Match: O04023 (Protein SRC2 homolog OS=Arabidopsis thaliana OX=3702 GN=SRC2 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.5e-24
Identity = 111/326 (34.05%), Postives = 146/326 (44.79%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAGQ---KFKTNADSTRGINPNWNFPMK 60
           ME  +L++T++SA DL ++ L+ K  +Y VVS+ G    K KT  D   G  P W   MK
Sbjct: 1   MECRSLDLTIISAEDLKDVQLIGKQDLYAVVSINGDARTKQKTKVDKDCGTKPKWKHQMK 60

Query: 61  FSINELASTQNREALVFTVRCSRGL-GDRDVGEVHVPVAELLRSAVDRN---SMRLVTYP 120
            ++++ A+  NR  LVF +   R + GD+ VGEV VPV ELL    D+N     + VTY 
Sbjct: 61  LTVDDAAARDNRLTLVFEIVADRPIAGDKPVGEVSVPVKELL----DQNKGDEEKTVTYA 120

Query: 121 VKKPSGKTQGVLSFEYKFGERVVAVGGS--------------VEKPAAGYAARGIGYPP- 180
           V+ P+GK +G L F +KFGE+      S              +++P   Y   G G P  
Sbjct: 121 VRLPNGKAKGSLKFSFKFGEKYTYGSSSGPHAPVPSAMDHKTMDQPVTAYPP-GHGAPSA 180

Query: 181 -PQPAAGYPSSYPP------------------YNA-----PPPNPQPYNYGYPHQPP--- 240
            P P AG  S YPP                  Y A     PPP   P   GYP  PP   
Sbjct: 181 YPAPPAGPSSGYPPQGHDDKHDGVYGYPQQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQ 240

Query: 241 ------PPPHHYGYS----PP-----------------PVVVEKKTSGMGKILGVGAALL 249
                 PP   YGY     PP                 P    K  +GMG  LG+GA LL
Sbjct: 241 GGYPGYPPQGPYGYPQQGYPPQGPYGYPQQQAHGKPQKPKKHGKAGAGMGLGLGLGAGLL 300

BLAST of Lag0016776 vs. ExPASy TrEMBL
Match: A0A6J1CB10 (protein SRC2-like OS=Momordica charantia OX=3673 GN=LOC111009847 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 2.7e-59
Identity = 167/297 (56.23%), Postives = 186/297 (62.63%), Query Frame = 0

Query: 1   MEST-TLEITVVSARDLPEINLVSKMVVYVVVSLAG-----QKFKTNADSTRGINPNWNF 60
           MEST +LEI VVSA+DLPEINLVSKM  YVVVSLAG     QKFKTNADS+ GINPNWNF
Sbjct: 1   MESTWSLEINVVSAKDLPEINLVSKMAAYVVVSLAGGGNSDQKFKTNADSSGGINPNWNF 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPV 120
           PMKFS+NELAST     LVFT+RC R LGDRD                       VTYPV
Sbjct: 61  PMKFSVNELAST-----LVFTIRCFRTLGDRD-----------------------VTYPV 120

Query: 121 KKPSGKTQGVLSFEYKFGERVVAVGGSVEKPAAGYAARGIG------YPPPQPAAGY-PS 180
           +KPSGKT G+L+F+Y+      +     +KP A YA RGIG       PPP PAAGY P 
Sbjct: 121 RKPSGKTHGLLNFQYRVLGATASFRDEKQKPPAAYATRGIGPPPEGYPPPPHPAAGYPPP 180

Query: 181 SYPPYN---APPPNPQPY---NYGYPHQPPPPPHHYGYSPPPVVVEKK--TSGMGKILGV 240
             PPYN   APPP PQPY   +YGYP  PPP     GY P   VV KK   SGMGKIL V
Sbjct: 181 PPPPYNTGYAPPP-PQPYGYPHYGYPAPPPP-----GYLPAAGVVAKKETKSGMGKILAV 240

Query: 241 GAALLGGVVCGALVGEMAADLGQASYSGGGTTHSTVAMSNSTLQSCAQSQNTLGQIG 277
           GA +LGG+ CGA++GE AADLGQ    G  + +  V  SN    S A SQNTLG IG
Sbjct: 241 GAGILGGLACGAMLGECAADLGQPPSRGSSSGNGGVHRSN--FSSMAYSQNTLGGIG 261

BLAST of Lag0016776 vs. ExPASy TrEMBL
Match: A0A7N2LUS8 (C2 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 8.1e-48
Identity = 135/275 (49.09%), Postives = 168/275 (61.09%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG------QKFKTNADSTRGINPNWNF 60
           ME  TLE+ VVSA+DL ++NL SKM VYVVVSL+G      QK KTN     G +P+WNF
Sbjct: 1   MEYRTLELNVVSAKDLKDVNLFSKMDVYVVVSLSGDSHHHKQKTKTNVAKDSGTSPSWNF 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPV 120
           PMKF+I E A+ QNR +L F +RC R LGD+D+G V+VP+ ELL S  D+ SM+ V+Y V
Sbjct: 61  PMKFTIEEYAAQQNRLSLEFKLRCDRSLGDKDIGVVYVPIQELLNSKGDK-SMKFVSYQV 120

Query: 121 KKPSGKTQGVLSFEYKFGERV--VAVGGSV--EKPAAGY----------AARGIGYPPPQ 180
           +KPSGK QG L+F YKFGE+V   AV   V  ++P   Y          A  G  YPPP 
Sbjct: 121 RKPSGKPQGELNFSYKFGEKVSNAAVAPKVYGDEPVTAYPVAKPVVGPSAPYGGSYPPPP 180

Query: 181 PAAGYPSSYPPYNAPPPNPQPYNYGYPHQPPPPPHHYGYSP------PPVVVEKKTSGMG 240
           P  G    YPP   PPP  QPY  GY   PPPPP  YGY P      PP V +++     
Sbjct: 181 PPPGPGYGYPP--PPPPYQQPYG-GY---PPPPPQGYGYPPGPGYGYPPPVQQQQPQKKN 240

Query: 241 KI-LGVGAALLGGVVCGALVGEMAADLGQASYSGG 249
           K  +G+GA LLGG + G L+G+M +D+G   Y GG
Sbjct: 241 KFGMGLGAGLLGGALGGLLIGDMVSDMG--DYGGG 266

BLAST of Lag0016776 vs. ExPASy TrEMBL
Match: A0A5J5C465 (Glutathione transferase OS=Nyssa sinensis OX=561372 GN=F0562_000604 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 5.2e-47
Identity = 130/285 (45.61%), Postives = 164/285 (57.54%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAGQ---KFKTNADSTRGINPNWNFPMK 60
           ME  TL+I ++SA+DL  +NL+ KM VYVVVS++G    K KT  D   G NP WNFPMK
Sbjct: 1   MEYRTLDINIISAKDLKNVNLIDKMDVYVVVSISGDKRTKQKTKVDRNGGTNPTWNFPMK 60

Query: 61  FSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPVKKP 120
           F+++E A+ QNR  L+FT+RC R LGD+D+GEVHVP+ ELL +  D   M+ V+Y V+KP
Sbjct: 61  FTVDESAAQQNRSTLMFTLRCERSLGDKDIGEVHVPIKELLGNIGD-GKMQFVSYQVRKP 120

Query: 121 SGKTQGVLSFEYKFGERVVAVGGSVEKPAAGYAARGI-----GYPP---------PQPAA 180
           SGK +G  +F YKFGE+V       ++P   Y A  +      YPP         P PA 
Sbjct: 121 SGKPKGEFNFSYKFGEKVAGAAAKADEPVTAYPAAPVAGTSSAYPPPPQYAAGPYPPPAG 180

Query: 181 GYP--SSYPP-----YNAPPPNPQPYNYGYP-------HQPPPPPHHYGYSP------PP 240
           GYP    Y P     Y  PPP PQP   GYP         PPPPP  YGY P       P
Sbjct: 181 GYPPHEGYAPPPVAGYGYPPP-PQPQYGGYPPPPPQYGGYPPPPPQGYGYPPQPGYGYQP 240

Query: 241 VVVEKKTSGMGKILGVGAALLGGVVCGALVGEMAADLGQASYSGG 249
           V   +K +  G  LG+GA LLGG + G L+G+M +D G   Y GG
Sbjct: 241 VQQPQKKNKFG--LGLGAGLLGGALGGLLIGDMISDAG--DYGGG 279

BLAST of Lag0016776 vs. ExPASy TrEMBL
Match: A0A2N9IAP9 (C2 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS49360 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 9.0e-47
Identity = 133/282 (47.16%), Postives = 165/282 (58.51%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG------QKFKTNADSTRGINPNWNF 60
           ME  TLE+ + SA+DL ++NL+SKM VYVVVSL G      QK KTN     G NP+WNF
Sbjct: 1   MEYRTLELNIASAKDLKDVNLISKMDVYVVVSLTGDSHNHKQKAKTNVSRDGGTNPSWNF 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPV 120
           PMKF+I E A+ QNR  L F +RC R LGD+D+GEV+VP+ ELL S  D  SM+ V Y V
Sbjct: 61  PMKFNIEEYAAQQNRLTLQFKLRCDRSLGDKDIGEVYVPIKELLNSRGDTKSMQFVAYQV 120

Query: 121 KKPSGKTQGVLSFEYKFGERV----VAVGGSVEKPAAGYAA------------RGIGYPP 180
           + PSGK++G L+F YKF E+V     A    V++P   Y A             G  YPP
Sbjct: 121 RTPSGKSKGELNFSYKFSEKVSGATAAPKAHVDEPVMAYPATAPSMVGPSAPYSGGSYPP 180

Query: 181 PQPAAGYPSSYPPYNAPPPNP--QPYNYGYPHQPPPPPHHYGYSP----------PPVVV 240
           P P  GY   YPP   PPP P  QPY  GYP  PPPPP  YGY P          P V  
Sbjct: 181 PPPGPGY--GYPP---PPPQPYQQPYG-GYP--PPPPPQGYGYPPGPGYGYPPQQPQVQQ 240

Query: 241 EKKTSGMGKILGVGAALLGGVVCGALVGEMAADLGQASYSGG 249
            +K +  G  +G+GA LLGGV+ G L+G++ +D G   + GG
Sbjct: 241 PQKKNKFG--MGMGAGLLGGVLGGLLIGDLVSDGG--GFDGG 270

BLAST of Lag0016776 vs. ExPASy TrEMBL
Match: A0A2P5ES55 (C2 domain containing protein OS=Trema orientale OX=63057 GN=TorRG33x02_158920 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 2.6e-46
Identity = 132/283 (46.64%), Postives = 169/283 (59.72%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG-----QKFKTNADSTRGINPNWNFP 60
           ME  +LEI V+SARDL ++NL++KM VY VVS++G     QK KTN D   G NP WNFP
Sbjct: 1   MELRSLEINVISARDLKDVNLMTKMDVYAVVSISGESHNHQKTKTNVDRASGRNPTWNFP 60

Query: 61  MKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNSMRLVTYPVK 120
            KF+++E A+ QNR  LVFT+ C R LGD+++G+V+VPV ELL S+   +SM+ V+Y V+
Sbjct: 61  AKFTVDESAAKQNRLTLVFTLFCDRTLGDKEIGQVNVPVKELLDSS-SSSSMQFVSYQVR 120

Query: 121 KPSGKTQGVLSFEYKFGERVVAVGGSVEKPAAGYAARGIG--------YPPP-------- 180
           KPSGK +G L F YKFGE++     + E P   Y A  +G        +PPP        
Sbjct: 121 KPSGKPKGELHFSYKFGEKLARKPDAGE-PITAYPAVEVGPSAPPTYLHPPPPAHGGYVP 180

Query: 181 -QPAAGYPSSYP-----PYNAPPPNPQPYNYGYPHQPPPPPH--------HYGYSPPPVV 240
            QP  GY   YP      Y A PP P P  YGYP  PPPPPH         YGY PP  V
Sbjct: 181 VQPVVGYGGGYPVPPAAGYGAYPPPPPPPGYGYP--PPPPPHGYGYPTGPGYGYPPPQQV 240

Query: 241 VEKKTSGMGKILGVGAALLGGVVCGALVGEMAADLGQASYSGG 249
            +KK +  G  +G+GA LLGG + G L+G+M +D   ASY  G
Sbjct: 241 GQKKNNKFG--MGLGAGLLGGALGGLLIGDMVSD--AASYDAG 275

BLAST of Lag0016776 vs. TAIR 10
Match: AT3G16510.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 119.0 bits (297), Expect = 6.0e-27
Identity = 111/357 (31.09%), Postives = 147/357 (41.18%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG------QKFKTNADSTRGINPNWNF 60
           M + TLE+ V SA+DL  +NL++KM VY VV + G       K KT  D T    P WN 
Sbjct: 1   MANLTLELNVYSAKDLENVNLITKMDVYAVVWITGDDSRKNHKEKTPIDRTGESEPTWNH 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAVDRNS-------M 120
            +KFS+++  + + R  LV  + C R  GD+D+GEV VPV ELL  +   +S       M
Sbjct: 61  TVKFSVDQRLAHEGRLTLVVKLVCDRIFGDKDLGEVQVPVLELLHGSSSPSSNGDGQGMM 120

Query: 121 RLVTYPVKKPSGKTQGVLSFEYKFGERVVAVGGSVEKPA--------------------A 180
           R VTY V+ P GK QG L+F Y+F          V                        +
Sbjct: 121 RFVTYQVRTPFGKGQGSLTFSYRFDSPTFKPDQPVSSHVFHQDPPVSSSHVYTNPMDIPS 180

Query: 181 GYAARGIGYPPPQ----------PAAGYPSSYPP--YNAPP---PNPQPYNYGYPHQP-- 240
            +++    YPPPQ           + GYP S PP  Y++PP   PNP  Y+  YP QP  
Sbjct: 181 DFSSATTNYPPPQSSEANFYPPLSSIGYPPSSPPQHYSSPPYPYPNPYQYHSHYPEQPVA 240

Query: 241 ----------------------------PPPP------------------------HHYG 242
                                       PPPP                        H YG
Sbjct: 241 VYPPPPPSASNLYPPPYYSTSPPQHQSYPPPPGHSFHQTQPSQSFHGFAPSSPQNQHGYG 300

BLAST of Lag0016776 vs. TAIR 10
Match: AT1G09070.1 (soybean gene regulated by cold-2 )

HSP 1 Score: 113.6 bits (283), Expect = 2.5e-25
Identity = 111/326 (34.05%), Postives = 146/326 (44.79%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAGQ---KFKTNADSTRGINPNWNFPMK 60
           ME  +L++T++SA DL ++ L+ K  +Y VVS+ G    K KT  D   G  P W   MK
Sbjct: 1   MECRSLDLTIISAEDLKDVQLIGKQDLYAVVSINGDARTKQKTKVDKDCGTKPKWKHQMK 60

Query: 61  FSINELASTQNREALVFTVRCSRGL-GDRDVGEVHVPVAELLRSAVDRN---SMRLVTYP 120
            ++++ A+  NR  LVF +   R + GD+ VGEV VPV ELL    D+N     + VTY 
Sbjct: 61  LTVDDAAARDNRLTLVFEIVADRPIAGDKPVGEVSVPVKELL----DQNKGDEEKTVTYA 120

Query: 121 VKKPSGKTQGVLSFEYKFGERVVAVGGS--------------VEKPAAGYAARGIGYPP- 180
           V+ P+GK +G L F +KFGE+      S              +++P   Y   G G P  
Sbjct: 121 VRLPNGKAKGSLKFSFKFGEKYTYGSSSGPHAPVPSAMDHKTMDQPVTAYPP-GHGAPSA 180

Query: 181 -PQPAAGYPSSYPP------------------YNA-----PPPNPQPYNYGYPHQPP--- 240
            P P AG  S YPP                  Y A     PPP   P   GYP  PP   
Sbjct: 181 YPAPPAGPSSGYPPQGHDDKHDGVYGYPQQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQ 240

Query: 241 ------PPPHHYGYS----PP-----------------PVVVEKKTSGMGKILGVGAALL 249
                 PP   YGY     PP                 P    K  +GMG  LG+GA LL
Sbjct: 241 GGYPGYPPQGPYGYPQQGYPPQGPYGYPQQQAHGKPQKPKKHGKAGAGMGLGLGLGAGLL 300

BLAST of Lag0016776 vs. TAIR 10
Match: AT4G15755.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-18
Identity = 88/287 (30.66%), Postives = 133/287 (46.34%), Query Frame = 0

Query: 1   MESTTLEITVVSARDLPEINLVSKMVVYVVVSLAG------QKFKTNADSTRGINPNWNF 60
           M + TLE+ + SAR+L  +NL++KM V+  +++ G      QK KT  D   G NP WN 
Sbjct: 1   MANLTLELNINSARNLLNVNLITKMNVFTAITINGENTRKKQKAKTTVDRYGGSNPTWNQ 60

Query: 61  PMKFSINELASTQNREALVFTVRCSRGLGDRDVGEVHVPVAELLRSAV-------DRNSM 120
            +KFS++E ++     +LV  V   R LG++++G V++P+ ELL +         + + M
Sbjct: 61  TIKFSVDERSARGGHSSLVMRVISRRVLGNKEIGRVNIPLLELLNATTPSFNGDGNDHEM 120

Query: 121 RLVTYPVKKPSGKTQGVLSFEYKFGERVVAV--GGSVEKPAAGYAARGIGYPPPQPAAGY 180
           +L++Y V+  SGK  G LSF Y+F   V  +     V+  A          PP  P    
Sbjct: 121 KLMSYQVRTSSGKRSGSLSFSYRFKPNVPVITNRSCVDSAAPSQIEHAPSAPPELPIEFP 180

Query: 181 PSSYPPYNAPPP---------NPQPY------------NY---GYPHQPPPPPHH----- 238
               PPY    P          P  Y            NY   G+ +   PPP       
Sbjct: 181 KLPQPPYLLRHPFAAGSSRGLLPISYGAGMTEQTGHANNYAQTGHANNYAPPPRQGYGPY 240

BLAST of Lag0016776 vs. TAIR 10
Match: AT4G15740.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.4e-07
Identity = 66/213 (30.99%), Postives = 98/213 (46.01%), Query Frame = 0

Query: 6   LEITVVSARDLPEINLVSKMVVYVVVSLAGQKFKTNADST-----RGINPNWNFPMKFSI 65
           LE+ +  A+++ ++N  S M VY  V++   +   N  +T        NP WN  MKFSI
Sbjct: 232 LELVIKFAKNIEDVNAFSSMDVYASVAILKDRKVKNRINTPVAFAAYTNPKWNQMMKFSI 291

Query: 66  NELASTQNREALVFTVRCSRG-LGDRDVGEVHVPVAELLRSAV--------DRNSMRLVT 125
           +E ++ + R  L+  +   R  LGD+++G V +P+ +LL S          D N M+L T
Sbjct: 292 DEKSAQEGRLMLLVELMSHRPFLGDKEIGFVRLPMQQLLVSNPPYPLTDNGDANGMKLET 351

Query: 126 YPVKKPSGKTQGVLSFEYKFGERVVAVGGSVEKPAAGYAARGIGYPPPQPAAG-----YP 185
           + +  P GK +GV+SF Y+F    V    +  +P   Y         PQP A        
Sbjct: 352 HALTGPYGK-KGVVSFTYRFLAEQVTAPSTPGQPYIMYVP-----VSPQPYASPDQLQVT 411

Query: 186 SSYPPYNAPPPNPQPYNYGYPHQPPPPPHHYGY 200
           SSY     P  N  P N   P   PP    +GY
Sbjct: 412 SSYVTVQ-PGANSGPSNGLVPIFMPPTYQSHGY 437