Lag0016696 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0016696 vs. NCBI nr
Match: XP_004151001.1 (probable aspartic proteinase GIP2 [Cucumis sativus] >KGN47256.1 hypothetical protein Csa_017018 [Cucumis sativus])

HSP 1 Score: 686.8 bits (1771), Expect = 1.2e-193
Identity = 359/419 (85.68%), Postives = 380/419 (90.69%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 60
           MAVP +P LLLLL    LFHG+TFSLVIP+TKDS+T+QY+A V HGSPIKP HLAVDLGG
Sbjct: 1   MAVPHFPFLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYVATVFHGSPIKPVHLAVDLGG 60

Query: 61  PSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 120
            SLWMAC GSSSSR IP RSIQC+AA GGG  GS      GGACDV+AGNPFG L GKA+
Sbjct: 61  QSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGS-----VGGACDVIAGNPFGDLEGKAI 120

Query: 121 LVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELG 180
           LVEDTVAVRS DRSTAAV+VA+HSCAPRFLLQGLAKS +G+LGLGRNQISLPAQIATELG
Sbjct: 121 LVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
            HRRFS+CLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKID  QSPEYFINVKAI
Sbjct: 181 SHRRFSLCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDALQSPEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDG-GDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNI 300
           KVDGNRLDLNKSLLDL+G GDGEG GGTRLSTVVPYT LESSIF SLTAAFRAAAAAMN+
Sbjct: 241 KVDGNRLDLNKSLLDLEGVGDGEGGGGTRLSTVVPYTVLESSIFNSLTAAFRAAAAAMNM 300

Query: 301 KEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLG 360
           KEVAPVAPFE CF +ENMEMTA GPKVPEIELILQSEMVGWKI GRNSMVKVNDEA+CLG
Sbjct: 301 KEVAPVAPFEVCFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAYCLG 360

Query: 361 FVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKTG 419
           FVDGGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSP NLL+ TG
Sbjct: 361 FVDGGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPVNLLESTG 414

BLAST of Lag0016696 vs. NCBI nr
Match: XP_022930452.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 681.4 bits (1757), Expect = 4.9e-192
Identity = 365/417 (87.53%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF  FHG TFSLVIPVTKDSVT+QYLA V+HGSPIKP HL VDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLVVDLGG 60

Query: 61  PSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 120
           PSLWMAC  S+SSR I  RSIQCVAA    GGGS SGS GGGAC+VVAGNPFGALVGKA+
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAAT---GGGSRSGS-GGGACEVVAGNPFGALVGKAL 120

Query: 121 LVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELG 180
           LVEDTVAVR+ +RSTAAV+VAVHSCAPRFLLQGLAKSA+GILGLGRNQISLPAQIATELG
Sbjct: 121 LVEDTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
           FHRRFSVCLSSTNGVVFPDS SQDSVYGSEISSSLTYTPILTK IDGSQS EYFINVKAI
Sbjct: 181 FHRRFSVCLSSTNGVVFPDSRSQDSVYGSEISSSLTYTPILTKIIDGSQSAEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIK 300
           KVDGNRLDLNKSLLDLDG DG   GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+K
Sbjct: 241 KVDGNRLDLNKSLLDLDGEDG---GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMK 300

Query: 301 EVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGF 360
           EVAPVA FEACF++ENMEMTA GPKVP IELILQSEMV WKI GRNSMVKVNDE FCL  
Sbjct: 301 EVAPVASFEACFSSENMEMTAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAI 360

Query: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 418
           VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lag0016696 vs. NCBI nr
Match: XP_038875838.1 (probable aspartic proteinase GIP2 [Benincasa hispida])

HSP 1 Score: 681.4 bits (1757), Expect = 4.9e-192
Identity = 358/410 (87.32%), Postives = 373/410 (90.98%), Query Frame = 0

Query: 9   LLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSLWMACA 68
           LLLLLCF  LFHG TFSLVIP+TKDSVT+ YLA V+HGSPIKP HLAVDLGG SLWMAC 
Sbjct: 10  LLLLLCFLTLFHGSTFSLVIPLTKDSVTNHYLATVYHGSPIKPLHLAVDLGGQSLWMACG 69

Query: 69  GSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAV 128
            SSSSR IP RSIQC+AA GGG  GS      GG CDV+A NPFG L GKAVLVEDTVA+
Sbjct: 70  ASSSSRSIPSRSIQCIAATGGGRSGS-----VGGVCDVIARNPFGDLEGKAVLVEDTVAL 129

Query: 129 RSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELGFHRRFSVC 188
           RS DRSTAAV+VA+HSCAPRFLLQGLAKS +GILGLGRNQISLPAQIATELG HRRFS+C
Sbjct: 130 RSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQIATELGSHRRFSIC 189

Query: 189 LSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLD 248
           LSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKID SQSPEYFINVKAIKVDGNRLD
Sbjct: 190 LSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVDGNRLD 249

Query: 249 LNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPF 308
           LNKSLLDLDGG   G GGTRLSTVVPYTALESSIF SL AAFRAAAAAMN+KEVAPVA F
Sbjct: 250 LNKSLLDLDGGGDGGGGGTRLSTVVPYTALESSIFNSLIAAFRAAAAAMNMKEVAPVALF 309

Query: 309 EACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGFVDGGLKPK 368
           EACF +ENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDEAFCLGFVDGGLKP+
Sbjct: 310 EACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDEAFCLGFVDGGLKPR 369

Query: 369 NAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKTG 419
           NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLK+TG
Sbjct: 370 NAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKRTG 414

BLAST of Lag0016696 vs. NCBI nr
Match: KAG7026255.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 680.6 bits (1755), Expect = 8.4e-192
Identity = 367/418 (87.80%), Postives = 381/418 (91.15%), Query Frame = 0

Query: 1   MAVPQYPSLLLLL-CFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLG 60
           MAVPQ  SLLLLL CFF  FHG TFSLVIPVTKDSVT+QYLA V+HGSPIKP HLAVDLG
Sbjct: 1   MAVPQSHSLLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLAVDLG 60

Query: 61  GPSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKA 120
           GPSLWMAC  S+SSR I  RSIQCVAA    GGGS SGS GGGAC+VVAGNPFGALVGKA
Sbjct: 61  GPSLWMACGSSTSSRTIQSRSIQCVAAT---GGGSRSGS-GGGACEVVAGNPFGALVGKA 120

Query: 121 VLVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATEL 180
           +LVEDTVAVR+ +RSTAAV+VAVHSCAPRFLLQGLAKSA+GILGLGRNQISLPAQIATEL
Sbjct: 121 LLVEDTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATEL 180

Query: 181 GFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKA 240
           GFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTK IDGSQS EYFINVKA
Sbjct: 181 GFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDGSQSAEYFINVKA 240

Query: 241 IKVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNI 300
           IKVDGNRLDLNKSLLDLDG DG   GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+
Sbjct: 241 IKVDGNRLDLNKSLLDLDGEDG---GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNM 300

Query: 301 KEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLG 360
           KEVAPVA FEACF++ENME+TA GPKVP IELILQSEMV WKI GRNSMVKVNDE FCL 
Sbjct: 301 KEVAPVASFEACFSSENMEITAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLA 360

Query: 361 FVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 418
            VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 IVDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 411

BLAST of Lag0016696 vs. NCBI nr
Match: XP_023515016.1 (basic 7S globulin-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.6 bits (1747), Expect = 7.1e-191
Identity = 364/417 (87.29%), Postives = 378/417 (90.65%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF  FHG TFSLVIPVTKDSVT+QYLA V+HGSPIKP  LAVDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIRLAVDLGG 60

Query: 61  PSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 120
           PSLWMAC  S+SSR I  RSIQCVAA    GGGS SGS GGGAC+VVAGN FGALVGKA+
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAAT---GGGSRSGS-GGGACEVVAGNSFGALVGKAL 120

Query: 121 LVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELG 180
           LVEDTVAVR+ +RSTAAV+VAVHSCAPRFLLQGLAKSA+GILGLGRNQISLPAQIATELG
Sbjct: 121 LVEDTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
           FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEIS+SLTYTPILTK IDGSQS EYFINVKAI
Sbjct: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISNSLTYTPILTKIIDGSQSAEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIK 300
           KVDGNRLDLNKS LDLDG DG   GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+K
Sbjct: 241 KVDGNRLDLNKSQLDLDGEDG---GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMK 300

Query: 301 EVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGF 360
           EVAPVA FEACFA+ENMEMTA GPKVP IELILQSEMV WKI GRNSMVKVNDE FCL  
Sbjct: 301 EVAPVASFEACFASENMEMTAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAI 360

Query: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 418
           VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lag0016696 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 6.4e-70
Identity = 166/406 (40.89%), Postives = 239/406 (58.87%), Query Frame = 0

Query: 26  LVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSLWMACAG---SSSSRPIPRRSIQ 85
           L++P+TKD++T QYL  +   +P+ P  L +DLGG  LW+ C     SS+ RP   RS Q
Sbjct: 34  LILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQ 93

Query: 86  C-VAAAGGGGGGSGSGSGGG---GACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAV 145
           C +A AG G G   S    G     C ++  N          L  DTV V+S +      
Sbjct: 94  CSLAGAGSGCGQCFSPPKPGCNNNTCGLLPDNTITRTATSGELASDTVQVQSSNGKNPGR 153

Query: 146 VVA----VHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELGFHRRFSVCLSS--- 205
            V+    +  C   FLL+GLA   +G+ GLGR +ISLP+Q + E  F R+F+VCLSS   
Sbjct: 154 HVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPRKFAVCLSSSTN 213

Query: 206 TNGVVFPDSGSQDSVYGSEISSS-LTYTPILTKKIDGSQ-------SPEYFINVKAIKVD 265
           + GVV    G    +   E +++  +YTP+    +  +        S EYFI VK+IK++
Sbjct: 214 SKGVVLFGDGPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSSEYFIGVKSIKIN 273

Query: 266 GNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVA 325
              + +N +LL +   D +GVGGT++STV PYT LE+SI+ ++T  F      +NI  VA
Sbjct: 274 EKVVPINTTLLSI---DNQGVGGTKISTVNPYTILETSIYNAVTNFF--VKELVNITRVA 333

Query: 326 PVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGFVDG 385
            VAPF ACF + N+  T  GP VP I+L+LQ+E V W+I G NSMV+V++   CLGFVDG
Sbjct: 334 SVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSENVLCLGFVDG 393

Query: 386 GLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 410
           G+ P+ ++V+GGY +ED +L FD+  S LGF+SS+L R+ +C+ F+
Sbjct: 394 GVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFN 434

BLAST of Lag0016696 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 223.8 bits (569), Expect = 3.7e-57
Identity = 156/432 (36.11%), Postives = 235/432 (54.40%), Query Frame = 0

Query: 4   PQYPSLL-LLLCFFILFHGETFS--LVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 63
           P  PSL    L    LF   TF   L+ P++KD  T  Y   V   +P++PT L + LG 
Sbjct: 3   PPLPSLCNFNLAILFLFLTPTFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGS 62

Query: 64  PSLWMACAG---SSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPF--GAL 123
              W+ C     SSSS  IP  +  C          S + S     C +   NP     L
Sbjct: 63  SLSWVLCDSTYTSSSSHHIPCNTPLC------NSFPSNACSNNSSLCALFPENPVTRNTL 122

Query: 124 VGKAVLVEDTVAVRSFDRSTAAVVVA--VHSCAPRFLLQGLAKSARGILGLGRNQISLPA 183
           +  A++  D++A+ ++D S++ V+++  + SCA   LLQGLA +A G+  LGR+  SLPA
Sbjct: 123 LDTALI--DSLALPTYDASSSLVLISDFIFSCATAHLLQGLAANALGLASLGRSNYSLPA 182

Query: 184 QIATELGFHRRFSVCL---SSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKI----- 243
           QI+T L   R F++CL   S+  G     S +   ++ S+I   LTYT ++   +     
Sbjct: 183 QISTSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKI--DLTYTQLIVNPVADTVV 242

Query: 244 -DGSQ-SPEYFINVKAIKVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIF 303
            D  Q S EYFIN+ +IK++G  L +N S+L +   D  G GGT++ST  PYT LE+SI+
Sbjct: 243 TDNPQPSDEYFINLTSIKINGKPLYINSSILTV---DQTGFGGTKISTAEPYTVLETSIY 302

Query: 304 KSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIA 363
           +     F   ++A N+     V PF  C+ A ++  T  GP VP ++L++ SE V W+I 
Sbjct: 303 RLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDVFWRIF 362

Query: 364 GRNSMVKV---NDEAFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQ 413
           G NSMV+V     + +CLGFVDGG + +  +V+GG+Q+ED ++ FD+ ++  GF+S+LL 
Sbjct: 363 GGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFTSTLLL 421

BLAST of Lag0016696 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 6.3e-49
Identity = 141/400 (35.25%), Postives = 208/400 (52.00%), Query Frame = 0

Query: 26  LVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSLWMACAG---SSSSRPIPRRSIQ 85
           LV+ V KD  T+ ++  +H  +P+      +DL G  L + C     SS+ +     S Q
Sbjct: 38  LVLKVQKDRATNLHVVQIHKRTPLVQFPFVIDLTGRFLSVNCENQYTSSTYKAPVCHSSQ 97

Query: 86  CVAAAGGGGGGSGSGSGGGG----ACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAV 145
           C  A         S     G    AC ++  NP      +  L ED + + S   S+   
Sbjct: 98  CARANSHTCRTCSSSKTRPGCHTNACGLLTTNPVTQQSAQGELAEDVLKIPSTQGSSPGP 157

Query: 146 VVA----VHSCAPRFLLQ-GLAKSARGILGLGRNQISLPAQIATELGFHRRFSVCLSST- 205
           +V     + +CAP  +LQ GL K+ +G+ GLG + ISLP Q+A+  GF  +F+VCL+S+ 
Sbjct: 158 MVTYPHFLFACAPSNILQKGLPKNVQGVAGLGHSPISLPYQLASHFGFPPKFAVCLTSSP 217

Query: 206 --NGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLN 265
             NG VF   G      G ++S  LTY P         Q  EY+INV++ K++      N
Sbjct: 218 GKNGAVFFGEGPYFMKPGIDVSRQLTYAPFTI-----GQQGEYYINVQSFKIN------N 277

Query: 266 KSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEA 325
             L  +  G   G GG  +ST  PYT L++ IF++L   F        +  V PVAPF A
Sbjct: 278 AMLPSIPKG---GFGGAMISTTTPYTTLQTPIFRALNQLF--MNQLRGVPHVKPVAPFGA 337

Query: 326 CFAAENMEMTATGPKVPEIELILQSEM-VGWKIAGRNSMVKVNDEAFCLGFVDGGLKPKN 385
           CF A  +  +  GP VP I+L+L ++  + W+I G N+M++      CL FVDGG++PK 
Sbjct: 338 CFDANRIPTSKMGPTVPSIDLVLDNKKNIMWRIFGANAMIQPRPGVMCLAFVDGGMRPKA 397

Query: 386 AVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 410
            +V+G  Q+ED +L FD+  S LGFSSSLL R+ +C+ F+
Sbjct: 398 PIVIGTQQLEDNLLQFDLMNSRLGFSSSLLFRRTNCANFN 421

BLAST of Lag0016696 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 9.4e-37
Identity = 125/412 (30.34%), Postives = 199/412 (48.30%), Query Frame = 0

Query: 26  LVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSLWMACAG--SSSSRPIP------ 85
           LV+P+ +D+ T  +   +   +P+    + +DL G  LW+ C+   SSS+   P      
Sbjct: 48  LVLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 107

Query: 86  ---RRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRS 145
                + QC           G  +     C +++ NP     G   L +D +A+ S   S
Sbjct: 108 CSRANTHQCFTCTDSTTSRPGCHN---NTCGLISSNPVTQESGLGELAQDVLALHSTHGS 167

Query: 146 TAAVVVAV----HSCAPRFLLQ-GLAKSARGILGLGRNQISLPAQIATELGFHRRFSVCL 205
               +V +     SCAP FL Q GL  + +G LGLG   ISLP Q+ +  G  R+F++CL
Sbjct: 168 KLGSLVKIPQFLFSCAPTFLTQKGLPNNVQGALGLGHAPISLPNQLFSHFGLKRQFTMCL 227

Query: 206 SS---TNGVV----FPDSGSQDSVYGS-EISSSLTYTPILTKKIDGSQSPEYFINVKAIK 265
           SS   +NG +      D  + + ++ S ++   + YTP+       S+  EYFI V AI+
Sbjct: 228 SSYPTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTPLTI-----SKQGEYFIQVSAIR 287

Query: 266 VDGNRL--DLNKSLLDLDGG----DGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAA 325
           V+ + +    N S+          +   +GG  ++T  PYT L  SIF+  T  F  A  
Sbjct: 288 VNKHMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLRHSIFEVFTQVF--ANN 347

Query: 326 AMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEA 385
                +V  V PF  C+  + +        VP ++LI+    V W+I+G N MV+  D  
Sbjct: 348 VPKQAQVKAVGPFGLCYDTKKI-----SGGVPSVDLIMDKSDVVWRISGENLMVQAQDGV 407

Query: 386 FCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 407
            CLGFVDGG+  +  + LG +Q+E+ ++ FD+  S +GF ++SL    +SCS
Sbjct: 408 SCLGFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTNSLKSHGKSCS 444

BLAST of Lag0016696 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 9.4e-37
Identity = 131/411 (31.87%), Postives = 199/411 (48.42%), Query Frame = 0

Query: 26  LVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSLWMACAG--SSSSRPIP------ 85
           LV+PV +D+ T  + A +H  +P+    L +DL G  LW+ C+   SSS+   P      
Sbjct: 47  LVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 106

Query: 86  ---RRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRS 145
                + QC           G  +     C +++ NP     G   L +D +A+ S   S
Sbjct: 107 CSRANTHQCFTCTDSTTTRPGCHN---NTCGLLSSNPVTQESGLGELAQDVLAIHSTHGS 166

Query: 146 TAAVVVAV----HSCAPRFLLQ-GLAKSARGILGLGRNQISLPAQIATELGFHRRFSVCL 205
               +V V     SCAP FL Q GL  + +G LGLG+  ISL  Q+ +  G  R+FSVCL
Sbjct: 167 KLGPMVKVPQFLFSCAPSFLAQKGLPNNVQGALGLGQAPISLQNQLFSHFGLKRQFSVCL 226

Query: 206 ---SSTNGVV----FPDSGSQDSVYGS-EISSSLTYTPILTKKIDGSQSPEYFINVKAIK 265
              S++NG +      D  + + ++ S ++   L YTP+       S+  EYFI V AI+
Sbjct: 227 SRYSTSNGAILFGDINDPNNNNYIHNSLDVLHDLVYTPLTI-----SKQGEYFIQVNAIR 286

Query: 266 VDGNRLDLNKSLLDLDG-----GDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAA 325
           V+ + +   K+           G GE +GG  ++T  PYT L  SIF+  T  F  A   
Sbjct: 287 VNKHLVIPTKNPFISPSSTSYHGSGE-IGGALITTTHPYTVLSHSIFEVFTQVF--ANNM 346

Query: 326 MNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAF 385
               +V  V PF  C+ +  +   A     P ++LIL      W+I+  N MV+  D   
Sbjct: 347 PKQAQVKAVGPFGLCYDSRKISGGA-----PSVDLILDKNDAVWRISSENFMVQAQDGVS 406

Query: 386 CLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 407
           CLGFVDGG+  +  + LG + +E+ ++ FD+  S +GF S+SL    ++CS
Sbjct: 407 CLGFVDGGVHARAGIALGAHHLEENLVVFDLERSRVGFNSNSLKSYGKTCS 441

BLAST of Lag0016696 vs. ExPASy TrEMBL
Match: A0A0A0KHJ8 (Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.6e-194
Identity = 359/419 (85.68%), Postives = 380/419 (90.69%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 60
           MAVP +P LLLLL    LFHG+TFSLVIP+TKDS+T+QY+A V HGSPIKP HLAVDLGG
Sbjct: 1   MAVPHFPFLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYVATVFHGSPIKPVHLAVDLGG 60

Query: 61  PSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 120
            SLWMAC GSSSSR IP RSIQC+AA GGG  GS      GGACDV+AGNPFG L GKA+
Sbjct: 61  QSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGS-----VGGACDVIAGNPFGDLEGKAI 120

Query: 121 LVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELG 180
           LVEDTVAVRS DRSTAAV+VA+HSCAPRFLLQGLAKS +G+LGLGRNQISLPAQIATELG
Sbjct: 121 LVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
            HRRFS+CLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKID  QSPEYFINVKAI
Sbjct: 181 SHRRFSLCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDALQSPEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDG-GDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNI 300
           KVDGNRLDLNKSLLDL+G GDGEG GGTRLSTVVPYT LESSIF SLTAAFRAAAAAMN+
Sbjct: 241 KVDGNRLDLNKSLLDLEGVGDGEGGGGTRLSTVVPYTVLESSIFNSLTAAFRAAAAAMNM 300

Query: 301 KEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLG 360
           KEVAPVAPFE CF +ENMEMTA GPKVPEIELILQSEMVGWKI GRNSMVKVNDEA+CLG
Sbjct: 301 KEVAPVAPFEVCFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAYCLG 360

Query: 361 FVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKTG 419
           FVDGGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSP NLL+ TG
Sbjct: 361 FVDGGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPVNLLESTG 414

BLAST of Lag0016696 vs. ExPASy TrEMBL
Match: A0A6J1EQJ3 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111436893 PE=4 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.4e-192
Identity = 365/417 (87.53%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF  FHG TFSLVIPVTKDSVT+QYLA V+HGSPIKP HL VDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLVVDLGG 60

Query: 61  PSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 120
           PSLWMAC  S+SSR I  RSIQCVAA    GGGS SGS GGGAC+VVAGNPFGALVGKA+
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAAT---GGGSRSGS-GGGACEVVAGNPFGALVGKAL 120

Query: 121 LVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELG 180
           LVEDTVAVR+ +RSTAAV+VAVHSCAPRFLLQGLAKSA+GILGLGRNQISLPAQIATELG
Sbjct: 121 LVEDTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
           FHRRFSVCLSSTNGVVFPDS SQDSVYGSEISSSLTYTPILTK IDGSQS EYFINVKAI
Sbjct: 181 FHRRFSVCLSSTNGVVFPDSRSQDSVYGSEISSSLTYTPILTKIIDGSQSAEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIK 300
           KVDGNRLDLNKSLLDLDG DG   GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+K
Sbjct: 241 KVDGNRLDLNKSLLDLDGEDG---GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMK 300

Query: 301 EVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGF 360
           EVAPVA FEACF++ENMEMTA GPKVP IELILQSEMV WKI GRNSMVKVNDE FCL  
Sbjct: 301 EVAPVASFEACFSSENMEMTAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAI 360

Query: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 418
           VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lag0016696 vs. ExPASy TrEMBL
Match: A0A6J1H4R8 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.1e-189
Identity = 358/421 (85.04%), Postives = 375/421 (89.07%), Query Frame = 0

Query: 1   MAVPQYPSLL---LLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVD 60
           MAVP+   LL   LLLCF  LFHG  FSLVIPV KDSVT+QYLA ++HGSPIKP HLAVD
Sbjct: 1   MAVPESRLLLLLRLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60

Query: 61  LGGPSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVG 120
           LGG  LWMAC GSSSSRPI  RSIQC+AA GGG  GS      G ACDV+AGNPFG L G
Sbjct: 61  LGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGS-----SGSACDVIAGNPFGDLEG 120

Query: 121 KAVLVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIAT 180
           KAVLVEDTVAVRS DRSTAAV+VA+HSCAPRFLLQGLA+SA+G+LGLGRNQISLPAQIAT
Sbjct: 121 KAVLVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAESAKGVLGLGRNQISLPAQIAT 180

Query: 181 ELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINV 240
           ELGFHRRFSVCLSSTNGVVFPDSG+ DSVYGSEISSSLTYTPILTKKID SQSPEYFINV
Sbjct: 181 ELGFHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINV 240

Query: 241 KAIKVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAM 300
           KAIKVDGNRLDLNKSLLDLDGG G   GGTRLSTVVPYT LESSIF SLTAAFRAAAAAM
Sbjct: 241 KAIKVDGNRLDLNKSLLDLDGGSG---GGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAM 300

Query: 301 NIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFC 360
           N+KEVAPVAPFEACF +ENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDE  C
Sbjct: 301 NMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLC 360

Query: 361 LGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 419
           LGFVDGGLKP+NAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK+SCSEFSPENL  + 
Sbjct: 361 LGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRA 413

BLAST of Lag0016696 vs. ExPASy TrEMBL
Match: A0A6J1KM09 (basic 7S globulin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE=4 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 9.3e-189
Identity = 360/417 (86.33%), Postives = 376/417 (90.17%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF  FHG TFSLVIPVTKDSVT+QYLA V+HGSPIKP HLAVDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLAVDLGG 60

Query: 61  PSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 120
           PSLWMAC  S+SSR I  RSIQCVAA    G GS SGS GGGAC+VVA NPFGALVGKA+
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAAT---GDGSRSGS-GGGACEVVARNPFGALVGKAL 120

Query: 121 LVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELG 180
           LVEDTVAVR+  RSTAAV+VAVHSCAPRFLLQGLAKSA+GILGLGRNQISLPAQIATELG
Sbjct: 121 LVEDTVAVRALARSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
           FHRRFSVCLSSTNGVVFP+SGSQDSVYGSEIS+SLTYTPILTK IDGSQS EYFINVKAI
Sbjct: 181 FHRRFSVCLSSTNGVVFPESGSQDSVYGSEISNSLTYTPILTKIIDGSQSAEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIK 300
           KVDGNRL L+KSLLDLD  DG   GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+K
Sbjct: 241 KVDGNRLVLDKSLLDLDSEDG---GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMK 300

Query: 301 EVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGF 360
           EVAPVA FE CFA+ENMEMTATGPKVP+IELILQSEMV WK  GRNSMVKVNDE FCL  
Sbjct: 301 EVAPVASFEVCFASENMEMTATGPKVPKIELILQSEMVRWKFDGRNSMVKVNDETFCLAI 360

Query: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 418
           VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lag0016696 vs. ExPASy TrEMBL
Match: A0A6J1KHR1 (basic 7S globulin-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE=4 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 9.3e-189
Identity = 360/417 (86.33%), Postives = 376/417 (90.17%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFILFHGETFSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF  FHG TFSLVIPVTKDSVT+QYLA V+HGSPIKP HLAVDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLAVDLGG 60

Query: 61  PSLWMACAGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 120
           PSLWMAC  S+SSR I  RSIQCVAA    G GS SGS GGGAC+VVA NPFGALVGKA+
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAAT---GDGSRSGS-GGGACEVVARNPFGALVGKAL 120

Query: 121 LVEDTVAVRSFDRSTAAVVVAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELG 180
           LVEDTVAVR+  RSTAAV+VAVHSCAPRFLLQGLAKSA+GILGLGRNQISLPAQIATELG
Sbjct: 121 LVEDTVAVRALARSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
           FHRRFSVCLSSTNGVVFP+SGSQDSVYGSEIS+SLTYTPILTK IDGSQS EYFINVKAI
Sbjct: 181 FHRRFSVCLSSTNGVVFPESGSQDSVYGSEISNSLTYTPILTKIIDGSQSAEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIK 300
           KVDGNRL L+KSLLDLD  DG   GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+K
Sbjct: 241 KVDGNRLVLDKSLLDLDSEDG---GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMK 300

Query: 301 EVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGF 360
           EVAPVA FE CFA+ENMEMTATGPKVP+IELILQSEMV WK  GRNSMVKVNDE FCL  
Sbjct: 301 EVAPVASFEVCFASENMEMTATGPKVPKIELILQSEMVRWKFDGRNSMVKVNDETFCLAI 360

Query: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 418
           VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 VDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lag0016696 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 263.8 bits (673), Expect = 2.3e-70
Identity = 170/423 (40.19%), Postives = 247/423 (58.39%), Query Frame = 0

Query: 8   SLLLLLCFFILFHGET----FSLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSL 67
           S+LLL  F +    +T     +L++PVTKD  T QY  +++  +P+ P  +  DLGG  L
Sbjct: 9   SVLLLFIFSLSSSAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGREL 68

Query: 68  WMACAGS--SSSRPIPR-RSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAV 127
           W+ C     SS+   PR  S  C  A     G   S    G + +   G P   + G A 
Sbjct: 69  WVDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNTCGGIPDNTVTGTAT 128

Query: 128 LVE---DTVAVRSFDRSTAAVVV----AVHSCAPRFLLQGLAKSARGILGLGRNQISLPA 187
             E   D V+++S + S    VV     +  C   FLL+GLAK   G+ G+GR+ I LP+
Sbjct: 129 SGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTVGMAGMGRHNIGLPS 188

Query: 188 QIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKI-------D 247
           Q A    FHR+F+VCL+S  GV F  +G    + G +I SSL  TP+L   +        
Sbjct: 189 QFAAAFSFHRKFAVCLTSGKGVAFFGNGPYVFLPGIQI-SSLQTTPLLINPVSTASAFSQ 248

Query: 248 GSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSL 307
           G +S EYFI V AI++    + +N +LL ++     G+GGT++S+V PYT LESSI+ + 
Sbjct: 249 GEKSSEYFIGVTAIQIVEKTVPINPTLLKINA--STGIGGTKISSVNPYTVLESSIYNAF 308

Query: 308 TAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRN 367
           T+ F   AAA +IK VA V PF ACF+ +N+ +T  G  VPEIEL+L S+ V W+I G N
Sbjct: 309 TSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIELVLHSKDVVWRIFGAN 368

Query: 368 SMVKVNDEAFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCS 410
           SMV V+D+  CLGFVDGG+  + +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+
Sbjct: 369 SMVSVSDDVICLGFVDGGVNARTSVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCA 428

BLAST of Lag0016696 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 253.4 bits (646), Expect = 3.1e-67
Identity = 162/402 (40.30%), Postives = 236/402 (58.71%), Query Frame = 0

Query: 25  SLVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSLWMACAGS--SSSRPIPR-RSI 84
           +L++PVTKD  T QY  +++  +P+ P  +  DLGG   W+ C     S++   PR  S 
Sbjct: 31  ALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLGGREFWVDCDQGYVSTTYRSPRCNSA 90

Query: 85  QCVAAAGGGGGGSGSGSGGGGACDVVAGNPFGALVGKAVLVE---DTVAVRSFDRSTAAV 144
            C  A     G   S    G + +     P  ++ G A   E   D V+++S + S    
Sbjct: 91  VCSRAGSIACGTCFSPPRPGCSNNTCGAFPDNSITGWATSGEFALDVVSIQSTNGSNPGR 150

Query: 145 VV----AVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELGFHRRFSVCLSSTNG 204
            V     + SC    LL+GLAK A G+ G+GR+ I LP Q A    F+R+F+VCL+S  G
Sbjct: 151 FVKIPNLIFSCGSTSLLKGLAKGAVGMAGMGRHNIGLPLQFAAAFSFNRKFAVCLTSGRG 210

Query: 205 VVFPDSGSQDSVYGSEISSSLTYTPILTKK-------IDGSQSPEYFINVKAIKVDGNRL 264
           V F  +G    + G +I S L  TP+L            G +SPEYFI V AIK+    L
Sbjct: 211 VAFFGNGPYVFLPGIQI-SRLQKTPLLINPGTTVFEFSKGEKSPEYFIGVTAIKIVEKTL 270

Query: 265 DLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAP 324
            ++ +LL ++     G+GGT++S+V PYT LESSI+K+ T+ F   AAA +IK VA V P
Sbjct: 271 PIDPTLLKINA--STGIGGTKISSVNPYTVLESSIYKAFTSEFIRQAAARSIKRVASVKP 330

Query: 325 FEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGFVDGGLKP 384
           F ACF+ +N+ +T  G  VPEI+L+L S+ V W+I G NSMV V+D+  CLGFVDGG+ P
Sbjct: 331 FGACFSTKNVGVTRLGYAVPEIQLVLHSKDVVWRIFGANSMVSVSDDVICLGFVDGGVNP 390

Query: 385 KNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 410
             +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+ F+
Sbjct: 391 GASVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCANFN 429

BLAST of Lag0016696 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 218.8 bits (556), Expect = 8.4e-57
Identity = 157/387 (40.57%), Postives = 203/387 (52.45%), Query Frame = 0

Query: 26  LVIPVTKDSVTDQYLAIVHHGSPIKPTHLAVDLGGPSLWMACAG---SSSSRPIPRRSIQ 85
           +V PV KD  T QYLA +  G    P  L VDL G  LW  C+    SSS   I   S  
Sbjct: 32  VVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSILWFDCSSRHVSSSRNLISGSSSG 91

Query: 86  CVAAAGGG---GGGSGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVV 145
           C+ A  G       S S       C+++  N    +  +  L  D ++V S   ++   V
Sbjct: 92  CLKAKVGNERVSSSSSSRKDQNADCELLVKNDAFGITARGELFSDVMSVGSV--TSPGTV 151

Query: 146 VAVHSCAPRFLLQGLAKSARGILGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPD 205
             + +C P +LL+GLA  A+G++GLGR QISLP+Q+A E    RR +V LS  NGVV   
Sbjct: 152 DLLFACTPPWLLRGLASGAQGVMGLGRAQISLPSQLAAETNERRRLTVYLSPLNGVV--S 211

Query: 206 SGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGG 265
           + S + V+G   S SL YTP+LT       S  Y INVK+I+V+G +L +          
Sbjct: 212 TSSVEEVFGVAASRSLVYTPLLT-----GSSGNYVINVKSIRVNGEKLSV---------- 271

Query: 266 DGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEM 325
             EG     LSTVVPYT LESSI+K    A+  A AA     V PVAPF  CF ++    
Sbjct: 272 --EGPLAVELSTVVPYTILESSIYKVFAEAY--AKAAGEATSVPPVAPFGLCFTSD---- 331

Query: 326 TATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDEAFCLGFVDGGLKPKNAVVLGGYQME 385
                  P ++L LQSEMV W+I G+N MV V     C G VDGG    N +V+GG Q+E
Sbjct: 332 ----VDFPAVDLALQSEMVRWRIHGKNLMVDVGGGVRCSGIVDGGSSRVNPIVMGGLQLE 383

Query: 386 DIVLDFDMGTSMLGFSSSLLQRKRSCS 407
             +LDFD+G SM+GF     QR RS S
Sbjct: 392 GFILDFDLGNSMMGFG----QRTRSDS 383

BLAST of Lag0016696 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 167.5 bits (423), Expect = 2.2e-41
Identity = 138/417 (33.09%), Postives = 197/417 (47.24%), Query Frame = 0

Query: 8   SLLLLLCFFILFHG----ETFSLVIPVTKDSVTDQYLAIVHHGSPIK-PTHLAVDLGGPS 67
           SL  LL F  +F           ++P+TK   T+ +    + GS  K P +L +DLG   
Sbjct: 4   SLTRLLVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNL 63

Query: 68  LWMAC---AGSSSSRPIPRRSIQCVAAAGGGGGGSGSGSGGGGACDVVAGNPFG---ALV 127
            W+ C      SS R +  +S  C +  G G  G         +C     NP G    + 
Sbjct: 64  TWLDCRKLKSLSSLRLVTCQSSTCKSIPGNGCAGK--------SCLYKQPNPLGQNPVVT 123

Query: 128 GKAVLVEDTVAVRSFDRSTAAVVVAVH----SCAPRFLLQGLAKSARGILGLGRNQISLP 187
           G+  +V+D  ++ + D       V+V     SCA    LQGL     G+L L     S  
Sbjct: 124 GR--VVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLALSPGSSSFT 183

Query: 188 AQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPE 247
            Q+ +      +FS+CL S+    F  +G     +    +SS    P     I G+ S +
Sbjct: 184 KQVTSAFNVIPKFSLCLPSSGTGHFYIAGIH--YFIPPFNSSDNPIPRTLTPIKGTDSGD 243

Query: 248 YFINVKAIKVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVPYTALESSIFKSLTAAFRA 307
           Y I VK+I V G  L LN  LL          GG +LSTVV YT L++ I+ +L  +F  
Sbjct: 244 YLITVKSIYVGGTALKLNPDLL---------TGGAKLSTVVHYTVLQTDIYNALAQSFTL 303

Query: 308 AAAAMNIKEVAPVAPFEACFAAENMEMTAT-GPKVPEIELILQSEM--VGWKIAGRNSMV 367
            A AM I +V  VAPF+ CF +       T GP VP IE+ L   +  V W   G N++V
Sbjct: 304 KAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPNVPVIEIGLPGRIGEVKWGFYGANTVV 363

Query: 368 KVNDEAFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCS 407
           KV +   CL F+DGG  PK+ +V+G +Q++D +L+FD   ++L FS SLL    SCS
Sbjct: 364 KVKETVMCLAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGTVLAFSESLLLHNTSCS 399

BLAST of Lag0016696 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 139.8 bits (351), Expect = 4.9e-33
Identity = 89/252 (35.32%), Postives = 134/252 (53.17%), Query Frame = 0

Query: 162 LGLGRNQISLPAQIATELGFHRRFSVCLSST------NGVVFPDSGSQDSV-YGSEISSS 221
           +GL    +S+P+Q+ +      + ++CL ST      NG ++   G    + Y  ++S  
Sbjct: 160 IGLANTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIGKGEYYYLPYDKDVSKI 219

Query: 222 LTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGDGEGVGGTRLSTVVP 281
              TP+    I   +S EY I+VK+I++    + +               G T++ST+ P
Sbjct: 220 FASTPL----IGNGKSGEYLIDVKSIQIGAKTVPI-------------PYGATKISTLAP 279

Query: 282 YTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQ 341
           YT  ++S++K+L  AF      + I +   V PF ACF +        G  VP I+L+L 
Sbjct: 280 YTVFQTSLYKALLTAF---TENIKIAKAPAVKPFGACFYSNG------GRGVPVIDLVL- 339

Query: 342 SEMVGWKIAGRNSMVKVNDEAFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF 401
           S    W+I G NS+VKVN    CLGFVDGG+KPK  +V+GG+QMED +++FD+  S   F
Sbjct: 340 SGGAKWRIYGSNSLVKVNKNVVCLGFVDGGVKPKYPIVIGGFQMEDNLVEFDLEASKFSF 384

Query: 402 SSSLLQRKRSCS 407
           SSSLL    SCS
Sbjct: 400 SSSLLLHNTSCS 384