Lag0016618 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016618
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr12: 39588689 .. 39589186 (-)
RNA-Seq ExpressionLag0016618
SyntenyLag0016618
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAAAGCTTAACACAGATGTTGCAATTGATAAAATAAATCAGCAGAGCAGCATGGGAGCTATTGTCCGAAATTGGAAAGGAGAAGTGATGCTTACCGCAATGAAGAGGATCCCGTTTGCACCCGATGTAGATTTGCTAGAGGCCTTGGCGGTTCGGGAAGGATTGAGTTGGGCAAAAGAGGCGGGGTTCACAAATCTGGTGGTTGAATCGGATTCCGCCCGAGTGGTTGCTTTGTTGAGATCTGAGACGATTAATATCTCAGAAGTCGGGGCGTTGGTGAAGGAAATTCAGCTCATAAGCAAGGAGCTGCACCTCTGTTTGTTCCGGTGGTGTAGAAGGGCAGCGAATGCAGTGGCCCATAAGCTGGCGAGAGTGGCGATGGAGAAGAAGAGTGAAGGTGTCTGGTTAGAAGAAGTTCCAAGGGGTATCGAAGGAGTTTATTTTTCTGAAATCTCAGACAAGCCCTCTAGCTTGTTGGAGGGTTTTGTGTCCTGA

mRNA sequence

ATGTTAAAGCTTAACACAGATGTTGCAATTGATAAAATAAATCAGCAGAGCAGCATGGGAGCTATTGTCCGAAATTGGAAAGGAGAAGTGATGCTTACCGCAATGAAGAGGATCCCGTTTGCACCCGATGTAGATTTGCTAGAGGCCTTGGCGGTTCGGGAAGGATTGAGTTGGGCAAAAGAGGCGGGGTTCACAAATCTGGTGGTTGAATCGGATTCCGCCCGAGTGGTTGCTTTGTTGAGATCTGAGACGATTAATATCTCAGAAGTCGGGGCGTTGGTGAAGGAAATTCAGCTCATAAGCAAGGAGCTGCACCTCTGTTTGTTCCGGTGGTGTAGAAGGGCAGCGAATGCAGTGGCCCATAAGCTGGCGAGAGTGGCGATGGAGAAGAAGAGTGAAGGTGTCTGGTTAGAAGAAGTTCCAAGGGGTATCGAAGGAGTTTATTTTTCTGAAATCTCAGACAAGCCCTCTAGCTTGTTGGAGGGTTTTGTGTCCTGA

Coding sequence (CDS)

ATGTTAAAGCTTAACACAGATGTTGCAATTGATAAAATAAATCAGCAGAGCAGCATGGGAGCTATTGTCCGAAATTGGAAAGGAGAAGTGATGCTTACCGCAATGAAGAGGATCCCGTTTGCACCCGATGTAGATTTGCTAGAGGCCTTGGCGGTTCGGGAAGGATTGAGTTGGGCAAAAGAGGCGGGGTTCACAAATCTGGTGGTTGAATCGGATTCCGCCCGAGTGGTTGCTTTGTTGAGATCTGAGACGATTAATATCTCAGAAGTCGGGGCGTTGGTGAAGGAAATTCAGCTCATAAGCAAGGAGCTGCACCTCTGTTTGTTCCGGTGGTGTAGAAGGGCAGCGAATGCAGTGGCCCATAAGCTGGCGAGAGTGGCGATGGAGAAGAAGAGTGAAGGTGTCTGGTTAGAAGAAGTTCCAAGGGGTATCGAAGGAGTTTATTTTTCTGAAATCTCAGACAAGCCCTCTAGCTTGTTGGAGGGTTTTGTGTCCTGA

Protein sequence

MLKLNTDVAIDKINQQSSMGAIVRNWKGEVMLTAMKRIPFAPDVDLLEALAVREGLSWAKEAGFTNLVVESDSARVVALLRSETINISEVGALVKEIQLISKELHLCLFRWCRRAANAVAHKLARVAMEKKSEGVWLEEVPRGIEGVYFSEISDKPSSLLEGFVS
Homology
BLAST of Lag0016618 vs. NCBI nr
Match: XP_023887146.1 (uncharacterized protein LOC111999255 [Quercus suber])

HSP 1 Score: 105.1 bits (261), Expect = 5.7e-19
Identity = 59/151 (39.07%), Postives = 85/151 (56.29%), Query Frame = 0

Query: 2   LKLNTDVAIDKINQQSSMGAIVRNWKGEVMLTAMKRIPFAPDVDLLEALAVREGLSWAKE 61
           +K+N D A       + +GAI+RN  G VM      IP    V+++E LA R  L +AKE
Sbjct: 92  VKINFDGATFSDLSSAGLGAIIRNDLGPVMAAFTHLIPLPTSVEMVEVLAARSALCFAKE 151

Query: 62  AGFTNLVVESDSARVVALLRSETINISEVGALVKEIQLISKELHLCLFRWCRRAANAVAH 121
            GF N++VE DS  V+  L +   + S +G +VK++++++  L    F   RR  N VAH
Sbjct: 152 LGFNNIIVEGDSEIVIKALSTNGPSFSSIGHIVKDVKVLASSLGNVSFNHTRRQGNRVAH 211

Query: 122 KLARVAMEKKSEGVWLEEVPRGIEGVYFSEI 153
            LAR+A        W+E+VP  +EGVY SEI
Sbjct: 212 GLARLACNFIHFQSWMEDVPPAVEGVYASEI 242

BLAST of Lag0016618 vs. NCBI nr
Match: XP_030946034.1 (uncharacterized protein LOC115970558 [Quercus lobata])

HSP 1 Score: 102.4 bits (254), Expect = 3.7e-18
Identity = 65/153 (42.48%), Postives = 86/153 (56.21%), Query Frame = 0

Query: 1   MLKLNTDVAIDKINQQSSMGAIVRNWKGEVMLTAMKRIPFAPDVDLLEALAVREGLSWAK 60
           M K+N D A+      S +G +VRN KGEVM +   R P   D D  E LA R  L +A 
Sbjct: 33  MYKVNFDAAVFADLDASGVGVVVRNDKGEVMASLAARGPPVRDSDEAETLACRRALEFAV 92

Query: 61  EAGFTNLVVESDSARVVALLRSETINISEVGALVKEIQLISKELHLCLFRWCRRAANAVA 120
           EAGF+ L++E D+  V+  L S   N S +G +V+EIQ IS  LH     + +R AN VA
Sbjct: 93  EAGFSELILEGDNLTVMKSLISLKPNRSRLGHIVEEIQCISAGLHRFAVSFVKRNANTVA 152

Query: 121 HKLARVAMEKKSEGVWLEEVPR-GIEGVYFSEI 153
           H LA+ A     E VWLEE P+  +E +YF  +
Sbjct: 153 HALAKYARLIDEELVWLEECPQPALEPLYFDSL 185

BLAST of Lag0016618 vs. NCBI nr
Match: XP_030934661.1 (uncharacterized protein LOC115960098 [Quercus lobata])

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-17
Identity = 57/151 (37.75%), Postives = 86/151 (56.95%), Query Frame = 0

Query: 2    LKLNTDVAIDKINQQSSMGAIVRNWKGEVMLTAMKRIPFAPDVDLLEALAVREGLSWAKE 61
            +K+N D A    +  + +GAI+RN  G VM    + IP    V+++E LA R  L +AK+
Sbjct: 1009 VKVNFDGATFGDSSSAGLGAIIRNDMGLVMAAFTQHIPLPTSVEMVEVLAARGALCFAKD 1068

Query: 62   AGFTNLVVESDSARVVALLRSETINISEVGALVKEIQLISKELHLCLFRWCRRAANAVAH 121
             GF  +VVE DS  ++  L S  ++ S  G ++++I+ +S  L   LF   RR  N VAH
Sbjct: 1069 LGFNKVVVEGDSEIIIKALSSNGLSASSFGHILQDIKSVSSSLGGVLFNHTRRQGNRVAH 1128

Query: 122  KLARVAMEKKSEGVWLEEVPRGIEGVYFSEI 153
             LAR+A    +   W+E+VP G+  VY SE+
Sbjct: 1129 GLARMACNFVNFQSWMEDVPPGVIDVYVSEV 1159

BLAST of Lag0016618 vs. NCBI nr
Match: XP_030936152.1 (uncharacterized protein LOC115961289 [Quercus lobata])

HSP 1 Score: 100.5 bits (249), Expect = 1.4e-17
Identity = 59/153 (38.56%), Postives = 87/153 (56.86%), Query Frame = 0

Query: 1   MLKLNTDVAIDKINQQSSMGAIVRNWKGEVMLTAMKRIPFAPDVDLLEALAVREGLSWAK 60
           + KLN D AI      S +G I+RN KGEVM     + P     +  E LA R  L +A 
Sbjct: 34  VFKLNFDAAIFSDLNGSGVGVIIRNCKGEVMAAMTTKGPLVGGSEEAEILACRRALEFAI 93

Query: 61  EAGFTNLVVESDSARVVALLRSETINISEVGALVKEIQLISKELHLCLFRWCRRAANAVA 120
           +AGFTNL+VE D+A V++ L S   ++S +G ++++IQ ++  +    F   +R AN VA
Sbjct: 94  DAGFTNLIVEGDNAAVMSSLSSSGADLSWLGHIIQDIQWLANGISWVCFSHVKRGANLVA 153

Query: 121 HKLARVAMEKKSEGVWLEEV-PRGIEGVYFSEI 153
           H LAR A +   + VWLE+  P  IE +Y+  +
Sbjct: 154 HVLARHAKDVTEDIVWLEDTPPPAIEALYYDSL 186

BLAST of Lag0016618 vs. NCBI nr
Match: XP_023915286.1 (uncharacterized protein LOC112026812 [Quercus suber])

HSP 1 Score: 100.1 bits (248), Expect = 1.8e-17
Identity = 58/139 (41.73%), Postives = 79/139 (56.83%), Query Frame = 0

Query: 3    KLNTDVAIDKINQQSSMGAIVRNWKGEVMLTAMKRIPFAPDVDLLEALAVREGLSWAKEA 62
            KLN DVA+      S +G I+RN  G+VM     R P   D D  E LA R GL +A EA
Sbjct: 912  KLNFDVAVFSDISASGVGVIIRNGDGQVMAALSARGPAVTDGDEAEVLACRRGLEFAMEA 971

Query: 63   GFTNLVVESDSARVVALLRSETINISEVGALVKEIQLISKELHLCLFRWCRRAANAVAHK 122
            GF +L+VE D+  V+  + S   + S +G LV +++ ++  L L  F+  RR+AN VAH 
Sbjct: 972  GFGDLIVEGDNLNVMQAVASNRSDWSRLGNLVDDVRHLAGRLRLVDFQCIRRSANGVAHS 1031

Query: 123  LARVAMEKKSEGVWLEEVP 142
            LAR A     + VWLE+ P
Sbjct: 1032 LARFARNISEDCVWLEDTP 1050

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023887146.15.7e-1939.07uncharacterized protein LOC111999255 [Quercus suber][more]
XP_030946034.13.7e-1842.48uncharacterized protein LOC115970558 [Quercus lobata][more]
XP_030934661.11.1e-1737.75uncharacterized protein LOC115960098 [Quercus lobata][more]
XP_030936152.11.4e-1738.56uncharacterized protein LOC115961289 [Quercus lobata][more]
XP_023915286.11.8e-1741.73uncharacterized protein LOC112026812 [Quercus suber][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 5..126
e-value: 5.2E-24
score: 84.4
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..135
e-value: 1.7E-16
score: 62.4
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 2..138
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 4..125
e-value: 5.90427E-22
score: 83.5176
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 2..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016618.1Lag0016618.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity