Lag0016573 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016573
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionserine/threonine-protein kinase BLUS1-like
Locationchr12: 39118102 .. 39119613 (-)
RNA-Seq ExpressionLag0016573
SyntenyLag0016573
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTACCATCAATGCCAAGATCAAGAATCCTCCACCAGCGAAGGAATCCATAATTTTCAGTTTCCGACGACCGCGGCCGGCTATCGAATCCTCAGTCAAATCGGCGTCAGCGACCACAGCGTCGTCTACATGGCGAACGTCAACGTTGAGTCTGCGACGGTCGTCGTCGCCGTAAAAGTTATCGACATCAACATCGACATCGACCCTGTATCTGTGACGAACTTGAACTCTCTATCACTAGTGAATCACGCCAATGTCCTCAGCCCTCACTGTTGTTTCACCGCCGACGACGGCCGCCTCTGGATCGTCATGCCGTTCATCCACGCCGGCCCTTTACAGGCCATACTCTCCAACCGTTTTCCGACCGGCTTGCCGGATCAATTCTTGGCCATTCTTTTTCACCAACTCCTCCGCGGCTTGGTTTGTCTTCACGAAATGGGCTTCGTCCATAAGGCCATCAAAGCAAGTAACGTTTTTTTCGACTCTCAAGGCTCCGTCAAGCTCTCCGATTTGAGCGTGTCGTCTAGTACAAAAGACGAAAACGACGACGACGATGAATACGACGAATCCTCGTCGGTACCGTATTGGGTTCCGGTGGACGAAGAGTATAGCTTCAAATCGGACGTGTGGTCGATTGGGATTTTAGCCATGGAAATTGCTTACGGGGGCCCTCCCATTTCCGACACAAGAATCATTAGGTCCCAAATTTCGAGCATCTCAAGGCGGTTTCCGTACGATTTGAATCTTGGTAAGACATCGGGAGATAGCAGCAGTAGTATAACCAATAGGAAATTTGGAGATTCGTTCAAGGATTTTGTGAGTTCTTGCCTCGTTAAAGATCAATTCAGAAGGCCTTCGGCTTCGAGCTTGTTGGCTCATCCATTCGTCAAGAAAGGGGAGAGTTCGAATTTCTTGTTCAAGAGCATCGCAAAAGGAATGGTTGGAATTGGAGATAAATTCCAACGAGATAAAGAAAGACTCGTGGGGTTGATGAAAAAGAAGAAGAACAACAGTAATTATTCTAGTAGTAAAAAAAATGTGAAAGTTGATAAGATTTTAAAGATTAGTGGATGGGATTACAACAACTTTTTGTTTGAGCTCGAGCCCGATGAGGAATCATTGGGGAAAAGGGTTCGATTTCGTGGAGAAACGATCATTCAATTTAGAGAAGATCAAGAGAGCAAAATTGATCATCATATGCCATTGGCAGCATCGCTAGTTGAAAGAATTGACGACAAGTTTGAGGTTATGGATAGTGAAAATTTCGCCAAAGCTTTGTCTTCTTTGTTAGAAAGTGTTGATCAACAAAGGAATGTGGTTATGATGTTGATAACTCAATTTGGTTATTTCAATCAAAGGGAGTTGGAAATGACGAAGGAAATTAGTAGATTAGAGGCAGAGTTGACCTCTGAGAAAGAAAAAAGCTTTCAATTGGAGTTGGATTATGAAGTTCTAAAGCTTAAACTTCCTGATTCTGAGGATGAAGAAGACCAAGAAGACAATTCTTGA

mRNA sequence

ATGGATTACCATCAATGCCAAGATCAAGAATCCTCCACCAGCGAAGGAATCCATAATTTTCAGTTTCCGACGACCGCGGCCGGCTATCGAATCCTCAGTCAAATCGGCGTCAGCGACCACAGCGTCGTCTACATGGCGAACGTCAACGTTGAGTCTGCGACGGTCGTCGTCGCCGTAAAAGTTATCGACATCAACATCGACATCGACCCTGTATCTGTGACGAACTTGAACTCTCTATCACTAGTGAATCACGCCAATGTCCTCAGCCCTCACTGTTGTTTCACCGCCGACGACGGCCGCCTCTGGATCGTCATGCCGTTCATCCACGCCGGCCCTTTACAGGCCATACTCTCCAACCGTTTTCCGACCGGCTTGCCGGATCAATTCTTGGCCATTCTTTTTCACCAACTCCTCCGCGGCTTGGTTTGTCTTCACGAAATGGGCTTCGTCCATAAGGCCATCAAAGCAAGTAACGTTTTTTTCGACTCTCAAGGCTCCGTCAAGCTCTCCGATTTGAGCGTGTCGTCTAGTACAAAAGACGAAAACGACGACGACGATGAATACGACGAATCCTCGTCGGTACCGTATTGGGTTCCGGTGGACGAAGAGTATAGCTTCAAATCGGACGTGTGGTCGATTGGGATTTTAGCCATGGAAATTGCTTACGGGGGCCCTCCCATTTCCGACACAAGAATCATTAGGTCCCAAATTTCGAGCATCTCAAGGCGGTTTCCGTACGATTTGAATCTTGGTAAGACATCGGGAGATAGCAGCAGTAGTATAACCAATAGGAAATTTGGAGATTCGTTCAAGGATTTTGTGAGTTCTTGCCTCGTTAAAGATCAATTCAGAAGGCCTTCGGCTTCGAGCTTGTTGGCTCATCCATTCGTCAAGAAAGGGGAGAGTTCGAATTTCTTGTTCAAGAGCATCGCAAAAGGAATGGTTGGAATTGGAGATAAATTCCAACGAGATAAAGAAAGACTCGTGGGGTTGATGAAAAAGAAGAAGAACAACAGTAATTATTCTAGTAGTAAAAAAAATGTGAAAGTTGATAAGATTTTAAAGATTAGTGGATGGGATTACAACAACTTTTTGTTTGAGCTCGAGCCCGATGAGGAATCATTGGGGAAAAGGGTTCGATTTCGTGGAGAAACGATCATTCAATTTAGAGAAGATCAAGAGAGCAAAATTGATCATCATATGCCATTGGCAGCATCGCTAGTTGAAAGAATTGACGACAAGTTTGAGGTTATGGATAGTGAAAATTTCGCCAAAGCTTTGTCTTCTTTGTTAGAAAGTGTTGATCAACAAAGGAATGTGGTTATGATGTTGATAACTCAATTTGGTTATTTCAATCAAAGGGAGTTGGAAATGACGAAGGAAATTAGTAGATTAGAGGCAGAGTTGACCTCTGAGAAAGAAAAAAGCTTTCAATTGGAGTTGGATTATGAAGTTCTAAAGCTTAAACTTCCTGATTCTGAGGATGAAGAAGACCAAGAAGACAATTCTTGA

Coding sequence (CDS)

ATGGATTACCATCAATGCCAAGATCAAGAATCCTCCACCAGCGAAGGAATCCATAATTTTCAGTTTCCGACGACCGCGGCCGGCTATCGAATCCTCAGTCAAATCGGCGTCAGCGACCACAGCGTCGTCTACATGGCGAACGTCAACGTTGAGTCTGCGACGGTCGTCGTCGCCGTAAAAGTTATCGACATCAACATCGACATCGACCCTGTATCTGTGACGAACTTGAACTCTCTATCACTAGTGAATCACGCCAATGTCCTCAGCCCTCACTGTTGTTTCACCGCCGACGACGGCCGCCTCTGGATCGTCATGCCGTTCATCCACGCCGGCCCTTTACAGGCCATACTCTCCAACCGTTTTCCGACCGGCTTGCCGGATCAATTCTTGGCCATTCTTTTTCACCAACTCCTCCGCGGCTTGGTTTGTCTTCACGAAATGGGCTTCGTCCATAAGGCCATCAAAGCAAGTAACGTTTTTTTCGACTCTCAAGGCTCCGTCAAGCTCTCCGATTTGAGCGTGTCGTCTAGTACAAAAGACGAAAACGACGACGACGATGAATACGACGAATCCTCGTCGGTACCGTATTGGGTTCCGGTGGACGAAGAGTATAGCTTCAAATCGGACGTGTGGTCGATTGGGATTTTAGCCATGGAAATTGCTTACGGGGGCCCTCCCATTTCCGACACAAGAATCATTAGGTCCCAAATTTCGAGCATCTCAAGGCGGTTTCCGTACGATTTGAATCTTGGTAAGACATCGGGAGATAGCAGCAGTAGTATAACCAATAGGAAATTTGGAGATTCGTTCAAGGATTTTGTGAGTTCTTGCCTCGTTAAAGATCAATTCAGAAGGCCTTCGGCTTCGAGCTTGTTGGCTCATCCATTCGTCAAGAAAGGGGAGAGTTCGAATTTCTTGTTCAAGAGCATCGCAAAAGGAATGGTTGGAATTGGAGATAAATTCCAACGAGATAAAGAAAGACTCGTGGGGTTGATGAAAAAGAAGAAGAACAACAGTAATTATTCTAGTAGTAAAAAAAATGTGAAAGTTGATAAGATTTTAAAGATTAGTGGATGGGATTACAACAACTTTTTGTTTGAGCTCGAGCCCGATGAGGAATCATTGGGGAAAAGGGTTCGATTTCGTGGAGAAACGATCATTCAATTTAGAGAAGATCAAGAGAGCAAAATTGATCATCATATGCCATTGGCAGCATCGCTAGTTGAAAGAATTGACGACAAGTTTGAGGTTATGGATAGTGAAAATTTCGCCAAAGCTTTGTCTTCTTTGTTAGAAAGTGTTGATCAACAAAGGAATGTGGTTATGATGTTGATAACTCAATTTGGTTATTTCAATCAAAGGGAGTTGGAAATGACGAAGGAAATTAGTAGATTAGAGGCAGAGTTGACCTCTGAGAAAGAAAAAAGCTTTCAATTGGAGTTGGATTATGAAGTTCTAAAGCTTAAACTTCCTGATTCTGAGGATGAAGAAGACCAAGAAGACAATTCTTGA

Protein sequence

MDYHQCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDINIDIDPVSVTNLNSLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYNNFLFELEPDEESLGKRVRFRGETIIQFREDQESKIDHHMPLAASLVERIDDKFEVMDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSEKEKSFQLELDYEVLKLKLPDSEDEEDQEDNS
Homology
BLAST of Lag0016573 vs. NCBI nr
Match: XP_022138626.1 (serine/threonine-protein kinase BLUS1-like [Momordica charantia])

HSP 1 Score: 581.3 bits (1497), Expect = 8.3e-162
Identity = 317/516 (61.43%), Postives = 391/516 (75.78%), Query Frame = 0

Query: 5   QCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDI 64
           Q QD E++ + G  +FQFPT A GY+IL QIGVSDHSVVY A     + +  VAVKVID+
Sbjct: 6   QDQDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDV 65

Query: 65  NIDIDPVSVTNLNSLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTG 124
           +  +D   V  +NSLSL++HAN+LSPHCCFT  D RLW VMPFIHAGPLQAIL+NRFP G
Sbjct: 66  DRHVDEFFVMKMNSLSLIDHANILSPHCCFTV-DRRLWTVMPFIHAGPLQAILANRFPNG 125

Query: 125 LPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDD 184
           LPDQFLAIL HQLLR L+CLHE+G+VH+ IKASN+F DS+GSVKLSDL+V       +DD
Sbjct: 126 LPDQFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVYVG----DDD 185

Query: 185 DDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRF 244
           DD+    SSVPYW P DEEYSFKSDVW+IGI+A+E+AYG PPISD+R IRSQ+ +ISRRF
Sbjct: 186 DDD----SSVPYWDPPDEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRF 245

Query: 245 PYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSN 304
           PY+LNL K    SS S+ +RKFG+ FKDFVSSCLVKD  +RPSA+SLLAHPF+KKGE+SN
Sbjct: 246 PYNLNLDKM---SSKSVIDRKFGELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSN 305

Query: 305 FLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYNNF 364
            LFKSIAK M GI D+F+++K+RLV LMKK +   + +          ++K+SGWD+NNF
Sbjct: 306 VLFKSIAKDMAGIEDRFEKNKDRLVELMKKDRRVEDNNRG-------NVVKVSGWDFNNF 365

Query: 365 LFELEPDEESLGKRVRFRGETIIQFREDQESKID------HHMP-------------LAA 424
           LFELEPDEESLGKRVRFRGETII  R+D  S  +       ++P             L+ 
Sbjct: 366 LFELEPDEESLGKRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSG 425

Query: 425 SLVERIDDKFEVMDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRL 484
            +  R +++  VMDSE  AK L+SL+ES+DQQR V+MM+I+QFG   +R++EMTKEI +L
Sbjct: 426 RMGSRAEEEVRVMDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKL 485

Query: 485 EAELTSEKEKSFQLELDYEVLKLKLPDSEDEEDQED 502
           +AEL  EKE+SFQLELDYEVLKLKLPDSEDE+  E+
Sbjct: 486 KAELAYEKERSFQLELDYEVLKLKLPDSEDEDKSEN 502

BLAST of Lag0016573 vs. NCBI nr
Match: XP_023006000.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita maxima])

HSP 1 Score: 555.8 bits (1431), Expect = 3.7e-154
Identity = 300/506 (59.29%), Postives = 377/506 (74.51%), Query Frame = 0

Query: 1   MDYHQCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVK 60
           MD  Q QDQE+S ++G H F+FP  A  Y I SQIG SDHS VY A      +T++VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTH-FKFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMIVAIK 60

Query: 61  VIDINIDIDPVSVTNLN-SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSN 120
           +ID N++IDP ++TN+N +LS +NH ++LSPHCCF A+DG LWIVMPFIHAGPLQAI+S 
Sbjct: 61  IIDSNVEIDPTAITNINTNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIISQ 120

Query: 121 RFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTK 180
           RF TGLPDQF++IL +QLLR LV LHE G VHK++KASN+FFD+ G V LSDL VS   K
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLSDLDVS---K 180

Query: 181 DENDDDDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISS 240
           D N D       SSVPYWVP +E+YS KSDVWS+GILAMEIAYG PPI D ++I++Q S 
Sbjct: 181 DMNKD------CSSVPYWVPPEEDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSK 240

Query: 241 ISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKK 300
           ISRRFPYDLNL   SG    S+  RKFGD+ K FV SCL KD F R SA SLL HPFVKK
Sbjct: 241 ISRRFPYDLNLDTRSG---KSVVPRKFGDTLKHFVGSCLAKDPFARVSALSLLDHPFVKK 300

Query: 301 GESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGW 360
           GE+SNFLFKSIAKGMVGIG+ F++DK+R++ LM   KN      S +     K+L+I+GW
Sbjct: 301 GENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLM---KNEVQLGESSQGGGGGKVLRITGW 360

Query: 361 DYNNFLFELEPDEESLGKRVRFRGETIIQFREDQE-SKIDHHMPLAASLVERIDDKFEV- 420
           DYNNFLFELEPDEES GKRVRFRGET+  ++E ++ +K D    + + +    D+K EV 
Sbjct: 361 DYNNFLFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFD----MKSLMHGGSDEKKEVD 420

Query: 421 --MDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSEKEK 480
             MDSE  A  LSSLL+++D Q+ +V ML+   G+ + ++LEM K+I RLE EL +EKEK
Sbjct: 421 QAMDSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEK 480

Query: 481 SFQLELDYEVLKLKLPDSEDEEDQED 502
           SFQLEL++E+LKLKLPDS++E++QE+
Sbjct: 481 SFQLELNFELLKLKLPDSDEEQEQEE 486

BLAST of Lag0016573 vs. NCBI nr
Match: XP_023548299.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.4 bits (1409), Expect = 1.3e-151
Identity = 294/509 (57.76%), Postives = 371/509 (72.89%), Query Frame = 0

Query: 1   MDYHQCQDQE------SSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESAT 60
           MD  Q QDQE      +S ++G H+ +FP  A  Y I SQIG SDHS VY A        
Sbjct: 1   MDRRQIQDQETSAASSASAAKGTHS-KFPKVADEYEIQSQIGASDHSTVYKAICKSADTP 60

Query: 61  VVVAVKVIDINIDIDPVSVTNLN-SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPL 120
            +VA+K+ID N++IDP ++TN+N +LS +NH ++L+PHCCFTA DGRLWIVMPFIHAGPL
Sbjct: 61  TIVAIKIIDSNVEIDPTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIHAGPL 120

Query: 121 QAILSNRFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLS 180
           QAI+S RF TGLPDQF++IL +QLLR LV LHE G VHKA+KASN+FFD+ G V LSDL 
Sbjct: 121 QAIISQRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLD 180

Query: 181 VSSSTKDENDDDDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRII 240
           VS   K+ N D       SSVPYW P +E+YSFKSDVWS+GILAMEIAYG PPI D ++I
Sbjct: 181 VS---KEMNKD------CSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVI 240

Query: 241 RSQISSISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLA 300
           ++Q S ISRRFPYDLNL   SG    S+  RKFGD+ K FV SCL KD+F R SA SLL 
Sbjct: 241 KAQFSKISRRFPYDLNLDNRSG---KSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLD 300

Query: 301 HPFVKKGESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKI 360
           HPFVKKGE+SNFLFKSIAKGMVGIG+ F++DK+R++ LMK +      S         K+
Sbjct: 301 HPFVKKGENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGG-----GKV 360

Query: 361 LKISGWDYNNFLFELEPDEESLGKRVRFRGETIIQFREDQE-SKIDHHMPLAASLVERID 420
           L+I+GWDYNNFLFELEPDEES GKRVRFRGET+  ++E ++ +K D    +     +   
Sbjct: 361 LRITGWDYNNFLFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKK 420

Query: 421 DKFEVMDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSE 480
           +  + MDSE  A  LSSLL+++D Q+ +V ML+   G+ + ++LEM K+I RLE EL +E
Sbjct: 421 EVDQAMDSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAE 480

Query: 481 KEKSFQLELDYEVLKLKLPDSEDEEDQED 502
           KEKSFQLEL++E+LKLKLPDS++E++QE+
Sbjct: 481 KEKSFQLELNFELLKLKLPDSDEEQEQEE 491

BLAST of Lag0016573 vs. NCBI nr
Match: XP_022958864.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita moschata])

HSP 1 Score: 545.4 bits (1404), Expect = 5.0e-151
Identity = 291/504 (57.74%), Postives = 369/504 (73.21%), Query Frame = 0

Query: 1   MDYHQCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVK 60
           MD  Q QDQE+S ++G H+ +FP  A  Y I SQIG SDHS VY A         +VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTHS-KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIK 60

Query: 61  VIDINIDIDPVSVTNLN-SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSN 120
           +ID N++ID  ++TN+N +LS +NH ++L+PHCCFTA DGRLWIVMPFI AGPLQAI+S 
Sbjct: 61  IIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQ 120

Query: 121 RFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTK 180
           RF TGLPDQF++IL +QLLR LV LHE G VHKA+KASN+FFD+ G V LSDL VS   K
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMK 180

Query: 181 DENDDDDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISS 240
                     + SSVPYW P +E+YSFKSDVWS+GILAMEIAYG PPI D ++I++Q S 
Sbjct: 181 ---------KDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSK 240

Query: 241 ISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKK 300
           ISRRFPYDLNL   SG    S+  RKFGD+ K FV SCL KD+F R SA SLL HPFVKK
Sbjct: 241 ISRRFPYDLNLDNRSG---KSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKK 300

Query: 301 GESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGW 360
           GE+SNFLFKSIAKGMVGIG+ F++DK+R++ LMK +      S         K+L+I+GW
Sbjct: 301 GENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGG-----GKVLRITGW 360

Query: 361 DYNNFLFELEPDEESLGKRVRFRGETIIQFREDQE-SKID-HHMPLAASLVERIDDKFEV 420
           DYNNFLFELEPDEES GKRVRFRGET+  ++E ++ +K D   +    S  E+  +  + 
Sbjct: 361 DYNNFLFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQA 420

Query: 421 MDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSEKEKSF 480
           MD+E  A  LSSLL+++D Q+ +V ML+   G+ + ++LEM K+I RLE EL +EKEKSF
Sbjct: 421 MDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSF 480

Query: 481 QLELDYEVLKLKLPDSEDEEDQED 502
           QLEL++E+LKLKLPDS++E++QE+
Sbjct: 481 QLELNFELLKLKLPDSDEEQEQEE 486

BLAST of Lag0016573 vs. NCBI nr
Match: KAG6574930.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 545.4 bits (1404), Expect = 5.0e-151
Identity = 291/504 (57.74%), Postives = 369/504 (73.21%), Query Frame = 0

Query: 1   MDYHQCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVK 60
           MD  Q QDQE+S ++G H+ +FP  A  Y I SQIG SDHS VY A         +VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTHS-KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIK 60

Query: 61  VIDINIDIDPVSVTNLN-SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSN 120
           +ID N++ID  ++TN+N +LS +NH ++L+PHCCFTA DGRLWIVMPFI AGPLQAI+S 
Sbjct: 61  IIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQ 120

Query: 121 RFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTK 180
           RF TGLPDQF++IL +QLLR LV LHE G VHKA+KASN+FFD+ G V LSDL VS   K
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMK 180

Query: 181 DENDDDDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISS 240
                     + SSVPYW P +E+YSFKSDVWS+GILAMEIAYG PPI D ++I++Q S 
Sbjct: 181 ---------KDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSK 240

Query: 241 ISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKK 300
           ISRRFPYDLNL   SG    S+  RKFGD+ K FV SCL KD+F R SA SLL HPFVKK
Sbjct: 241 ISRRFPYDLNLDNRSG---KSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKK 300

Query: 301 GESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGW 360
           GE+SNFLFKSIAKGMVGIG+ F++DK+R++ LMK +      S         K+L+I+GW
Sbjct: 301 GENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGG-----GKVLRITGW 360

Query: 361 DYNNFLFELEPDEESLGKRVRFRGETIIQFREDQE-SKID-HHMPLAASLVERIDDKFEV 420
           DYNNFLFELEPDEES GKRVRFRGET+  ++E ++ +K D   +    S  E+  +  + 
Sbjct: 361 DYNNFLFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQA 420

Query: 421 MDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSEKEKSF 480
           MD+E  A  LSSLL+++D Q+ +V ML+   G+ + ++LEM K+I RLE EL +EKEKSF
Sbjct: 421 MDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSF 480

Query: 481 QLELDYEVLKLKLPDSEDEEDQED 502
           QLEL++E+LKLKLPDS++E++QE+
Sbjct: 481 QLELNFELLKLKLPDSDEEQEQEE 486

BLAST of Lag0016573 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.2e-51
Identity = 146/490 (29.80%), Postives = 254/490 (51.84%), Query Frame = 0

Query: 18  HNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDIN---IDIDPVSVT 77
           +  +FP  A  Y I+ +IGV   + VY A + +   ++VVA+K ID++    D D +   
Sbjct: 4   NKLEFPLDAEAYEIICKIGVGVSASVYKA-ICIPMNSMVVAIKAIDLDQSRADFDSLR-R 63

Query: 78  NLNSLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILF 137
              ++SL++H N+L+ +C FT  D  LW+VMPF+  G L +I+S+ FP+GLP+  +++  
Sbjct: 64  ETKTMSLLSHPNILNAYCSFTV-DRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFL 123

Query: 138 HQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESS-- 197
            + L  +  LH+ G +H+ IKA N+  DS GSVKL+D  VS+S  +          SS  
Sbjct: 124 KETLNAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLR 183

Query: 198 -----SVPYWVPVD-----EEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISR 257
                  PYW+  +       Y FK+D+WS GI A+E+A+G PP+S    ++S +  I++
Sbjct: 184 LTDIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITK 243

Query: 258 RFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGES 317
           RF +      TSG S     N+KF  +F++ V  CL +D  +RPSA  LL HPF K  + 
Sbjct: 244 RFHFSDYEINTSGSSKKG--NKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKG 303

Query: 318 SNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYN 377
            +F+ K++   +      F   +     ++ K   + +    +++ ++ K  +ISGW++ 
Sbjct: 304 LDFVVKNVLHSLSNAEQMFMESQ-----ILIKSVGDDDEEEEEEDEEIVKNRRISGWNFR 363

Query: 378 NFLFELEP-----DEESLGKRVRFRGETIIQFREDQESKIDHHMPLAASLVERIDDKFEV 437
               +L P     + +S     R   ++  +  +D  +   + + L  S  E  + + EV
Sbjct: 364 EDDLQLSPVFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAKNQEGEV 423

Query: 438 M--DSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQ---RELEMTKEISRLEAELTSE 483
           +  D +   + L  L +S++ QR  V ++I       +   RE E+ + + +L+ EL +E
Sbjct: 424 VGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELLEMVEKLKIELETE 483

BLAST of Lag0016573 vs. ExPASy Swiss-Prot
Match: P97820 (Mitogen-activated protein kinase kinase kinase kinase 4 OS=Mus musculus OX=10090 GN=Map4k4 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.4e-23
Identity = 81/285 (28.42%), Postives = 130/285 (45.61%), Query Frame = 0

Query: 29  YRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDINIDIDPVSVTNLNSL-SLVNHANV 88
           + ++  +G   +  VY         TV  A+KV+D+  D +      +N L    +H N+
Sbjct: 25  FELVEVVGNGTYGQVYKGR---HVKTVTAAIKVMDVTEDEEEEITLEINMLKKYSHHRNI 84

Query: 89  LSPHCCF-----TADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQLLRGLV 148
            + +  F        D +LW+VM F  AG +  ++ N     L + ++A +  ++LRGL 
Sbjct: 85  ATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLA 144

Query: 149 CLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESSSVPYWVP--- 208
            LH    +H+ IK  NV       VKL D  VS+         + +      PYW+    
Sbjct: 145 HLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF---IGTPYWMAPEV 204

Query: 209 ------VDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGKT 268
                  D  Y ++SD+WS GI A+E+A GGPP+ D   +R+ +  I R  P        
Sbjct: 205 IACDENPDATYDYRSDLWSCGITAIEMAEGGPPLCDMHPMRA-LFLIPRNPP-------- 264

Query: 269 SGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVK 299
                  + ++K+   F  F+  CLVK+  +RPS   LL HPF++
Sbjct: 265 -----PRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIR 289

BLAST of Lag0016573 vs. ExPASy Swiss-Prot
Match: Q9UKE5 (TRAF2 and NCK-interacting protein kinase OS=Homo sapiens OX=9606 GN=TNIK PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.2e-22
Identity = 103/408 (25.25%), Postives = 183/408 (44.85%), Query Frame = 0

Query: 29  YRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDINIDIDPVSVTNLNSL-SLVNHANV 88
           + ++  +G   +  VY    +V++   + A+KV+D+  D +      +N L    +H N+
Sbjct: 25  FELVELVGNGTYGQVYKGR-HVKTGQ-LAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNI 84

Query: 89  LSPHCCFTAD-----DGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQLLRGLV 148
            + +  F        D +LW+VM F  AG +  ++ N     L ++++A +  ++LRGL 
Sbjct: 85  ATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLS 144

Query: 149 CLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESSSVPYWVP--- 208
            LH+   +H+ IK  NV       VKL D  VS+         + +      PYW+    
Sbjct: 145 HLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF---IGTPYWMAPEV 204

Query: 209 ------VDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGKT 268
                  D  Y FKSD+WS+GI A+E+A G PP+ D   +R+              L   
Sbjct: 205 IACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRA--------------LFLI 264

Query: 269 SGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSN----FLFKS 328
             + +  + ++K+   F+ F+ SCLVK+  +RP+   L+ HPF++   +       L   
Sbjct: 265 PRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDH 324

Query: 329 IAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYNNFL-FEL 388
           I +     G+K + + E      ++++N+S   SS  N+  +  L+       +FL  +L
Sbjct: 325 IDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLR------RDFLRLQL 384

Query: 389 EPDEESLGKRVRFRGETIIQFREDQESKIDHHMPLAASLVERIDDKFE 417
              E S   R +       Q  + Q    +H   L A   +RI+++ E
Sbjct: 385 ANKERSEALRRQ-------QLEQQQRENEEHKRQLLAERQKRIEEQKE 400

BLAST of Lag0016573 vs. ExPASy Swiss-Prot
Match: P83510 (Traf2 and NCK-interacting protein kinase OS=Mus musculus OX=10090 GN=Tnik PE=1 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 1.0e-21
Identity = 115/484 (23.76%), Postives = 213/484 (44.01%), Query Frame = 0

Query: 29  YRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDINIDIDPVSVTNLNSL-SLVNHANV 88
           + ++  +G   +  VY    +V++   + A+KV+D+  D +      +N L    +H N+
Sbjct: 25  FELVELVGNGTYGQVYKGR-HVKTGQ-LAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNI 84

Query: 89  LSPHCCFTAD-----DGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQLLRGLV 148
            + +  F        D +LW+VM F  AG +  ++ N     L ++++A +  ++LRGL 
Sbjct: 85  ATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLS 144

Query: 149 CLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESSSVPYWVP--- 208
            LH+   +H+ IK  NV       VKL D  VS+         + +      PYW+    
Sbjct: 145 HLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF---IGTPYWMAPEV 204

Query: 209 ------VDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGKT 268
                  D  Y FKSD+WS+GI A+E+A G PP+ D   +R+              L   
Sbjct: 205 IACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRA--------------LFLI 264

Query: 269 SGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSN----FLFKS 328
             + +  + ++K+   F+ F+ SCLVK+  +RP+   L+ HPF++   +       L   
Sbjct: 265 PRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDH 324

Query: 329 IAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYNNFL-FEL 388
           I +     G+K + + E      ++++N+S   SS  N+  +  L+       +FL  +L
Sbjct: 325 IDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLR------RDFLRLQL 384

Query: 389 EPDEESLGKRVRFRGETIIQFREDQESKIDHHMPLAASLVERIDDKFE----VMDSENFA 448
              E S   R +       Q  + Q    +H   L A   +RI+++ E    + + +   
Sbjct: 385 ANKERSEALRRQ-------QLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRRE 444

Query: 449 KALSSLLESVDQQRNVVMML--------ITQFGYFNQRELEMTKEISRLEAELTSEKEKS 481
           K L    E  +Q+R+    +              + +++LE  ++  RL+ +L  E++  
Sbjct: 445 KELRKQQER-EQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYL 475

BLAST of Lag0016573 vs. ExPASy Swiss-Prot
Match: Q9JM52 (Misshapen-like kinase 1 OS=Mus musculus OX=10090 GN=Mink1 PE=1 SV=3)

HSP 1 Score: 101.3 bits (251), Expect = 3.3e-20
Identity = 112/495 (22.63%), Postives = 211/495 (42.63%), Query Frame = 0

Query: 29  YRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDINIDIDPVSVTNLNSL-SLVNHANV 88
           + ++  +G   +  VY    +V++   + A+KV+D+  D +      +N L    +H N+
Sbjct: 25  FELVEVVGNGTYGQVYKGR-HVKTGQ-LAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNI 84

Query: 89  LSPHCCF-----TADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQLLRGLV 148
            + +  F       +D +LW+VM F  AG +  ++ N     L +  +A +  ++LRGL 
Sbjct: 85  ATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLA 144

Query: 149 CLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESSSVPYWVP--- 208
            LH    +H+ IK  NV       VKL D  VS+         + +      PYW+    
Sbjct: 145 HLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF---IGTPYWMAPEV 204

Query: 209 ------VDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGKT 268
                  D  Y ++SD+WS+GI A+E+A G PP+ D   +R+ +  I R  P        
Sbjct: 205 IACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRA-LFLIPRNPP-------- 264

Query: 269 SGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSNFLFKSIAKG 328
                  + ++K+   F DF+ +CL+K    RP    LL  PF++   +         + 
Sbjct: 265 -----PRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTER-------QV 324

Query: 329 MVGIGDKFQRDKERLVGLMKKKKNNSNYSSSK-------KNVKVDKILKISGWD-YNNFL 388
            + + D   R +++     +K++    YS S+       +  +   I+ + G        
Sbjct: 325 RIQLKDHIDRSRKK---RGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREF 384

Query: 389 FELEPDEESLGKRVRFRGETIIQFREDQESKIDHHM-PLAASLVERIDDKFEVMDSENFA 448
             L+ + +S  + ++ + +   Q + D E+ I H +      + E+ +++  V + +   
Sbjct: 385 LRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRRE 444

Query: 449 KALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSEKEKSFQLELDYE 500
           +    L E   Q+R   M  + +     Q E E   +  +LE +  SE+    Q +L  E
Sbjct: 445 REQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSER---LQRQLQQE 487

BLAST of Lag0016573 vs. ExPASy TrEMBL
Match: A0A6J1CA92 (serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC111009741 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 4.0e-162
Identity = 317/516 (61.43%), Postives = 391/516 (75.78%), Query Frame = 0

Query: 5   QCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDI 64
           Q QD E++ + G  +FQFPT A GY+IL QIGVSDHSVVY A     + +  VAVKVID+
Sbjct: 6   QDQDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDV 65

Query: 65  NIDIDPVSVTNLNSLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTG 124
           +  +D   V  +NSLSL++HAN+LSPHCCFT  D RLW VMPFIHAGPLQAIL+NRFP G
Sbjct: 66  DRHVDEFFVMKMNSLSLIDHANILSPHCCFTV-DRRLWTVMPFIHAGPLQAILANRFPNG 125

Query: 125 LPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDD 184
           LPDQFLAIL HQLLR L+CLHE+G+VH+ IKASN+F DS+GSVKLSDL+V       +DD
Sbjct: 126 LPDQFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVYVG----DDD 185

Query: 185 DDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRF 244
           DD+    SSVPYW P DEEYSFKSDVW+IGI+A+E+AYG PPISD+R IRSQ+ +ISRRF
Sbjct: 186 DDD----SSVPYWDPPDEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRF 245

Query: 245 PYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSN 304
           PY+LNL K    SS S+ +RKFG+ FKDFVSSCLVKD  +RPSA+SLLAHPF+KKGE+SN
Sbjct: 246 PYNLNLDKM---SSKSVIDRKFGELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSN 305

Query: 305 FLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYNNF 364
            LFKSIAK M GI D+F+++K+RLV LMKK +   + +          ++K+SGWD+NNF
Sbjct: 306 VLFKSIAKDMAGIEDRFEKNKDRLVELMKKDRRVEDNNRG-------NVVKVSGWDFNNF 365

Query: 365 LFELEPDEESLGKRVRFRGETIIQFREDQESKID------HHMP-------------LAA 424
           LFELEPDEESLGKRVRFRGETII  R+D  S  +       ++P             L+ 
Sbjct: 366 LFELEPDEESLGKRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSG 425

Query: 425 SLVERIDDKFEVMDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRL 484
            +  R +++  VMDSE  AK L+SL+ES+DQQR V+MM+I+QFG   +R++EMTKEI +L
Sbjct: 426 RMGSRAEEEVRVMDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKL 485

Query: 485 EAELTSEKEKSFQLELDYEVLKLKLPDSEDEEDQED 502
           +AEL  EKE+SFQLELDYEVLKLKLPDSEDE+  E+
Sbjct: 486 KAELAYEKERSFQLELDYEVLKLKLPDSEDEDKSEN 502

BLAST of Lag0016573 vs. ExPASy TrEMBL
Match: A0A6J1KYZ4 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498878 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.8e-154
Identity = 300/506 (59.29%), Postives = 377/506 (74.51%), Query Frame = 0

Query: 1   MDYHQCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVK 60
           MD  Q QDQE+S ++G H F+FP  A  Y I SQIG SDHS VY A      +T++VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTH-FKFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMIVAIK 60

Query: 61  VIDINIDIDPVSVTNLN-SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSN 120
           +ID N++IDP ++TN+N +LS +NH ++LSPHCCF A+DG LWIVMPFIHAGPLQAI+S 
Sbjct: 61  IIDSNVEIDPTAITNINTNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIISQ 120

Query: 121 RFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTK 180
           RF TGLPDQF++IL +QLLR LV LHE G VHK++KASN+FFD+ G V LSDL VS   K
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLSDLDVS---K 180

Query: 181 DENDDDDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISS 240
           D N D       SSVPYWVP +E+YS KSDVWS+GILAMEIAYG PPI D ++I++Q S 
Sbjct: 181 DMNKD------CSSVPYWVPPEEDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSK 240

Query: 241 ISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKK 300
           ISRRFPYDLNL   SG    S+  RKFGD+ K FV SCL KD F R SA SLL HPFVKK
Sbjct: 241 ISRRFPYDLNLDTRSG---KSVVPRKFGDTLKHFVGSCLAKDPFARVSALSLLDHPFVKK 300

Query: 301 GESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGW 360
           GE+SNFLFKSIAKGMVGIG+ F++DK+R++ LM   KN      S +     K+L+I+GW
Sbjct: 301 GENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLM---KNEVQLGESSQGGGGGKVLRITGW 360

Query: 361 DYNNFLFELEPDEESLGKRVRFRGETIIQFREDQE-SKIDHHMPLAASLVERIDDKFEV- 420
           DYNNFLFELEPDEES GKRVRFRGET+  ++E ++ +K D    + + +    D+K EV 
Sbjct: 361 DYNNFLFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFD----MKSLMHGGSDEKKEVD 420

Query: 421 --MDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSEKEK 480
             MDSE  A  LSSLL+++D Q+ +V ML+   G+ + ++LEM K+I RLE EL +EKEK
Sbjct: 421 QAMDSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEK 480

Query: 481 SFQLELDYEVLKLKLPDSEDEEDQED 502
           SFQLEL++E+LKLKLPDS++E++QE+
Sbjct: 481 SFQLELNFELLKLKLPDSDEEQEQEE 486

BLAST of Lag0016573 vs. ExPASy TrEMBL
Match: A0A6J1H6C5 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460018 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 2.4e-151
Identity = 291/504 (57.74%), Postives = 369/504 (73.21%), Query Frame = 0

Query: 1   MDYHQCQDQESSTSEGIHNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVK 60
           MD  Q QDQE+S ++G H+ +FP  A  Y I SQIG SDHS VY A         +VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTHS-KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIK 60

Query: 61  VIDINIDIDPVSVTNLN-SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSN 120
           +ID N++ID  ++TN+N +LS +NH ++L+PHCCFTA DGRLWIVMPFI AGPLQAI+S 
Sbjct: 61  IIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQ 120

Query: 121 RFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTK 180
           RF TGLPDQF++IL +QLLR LV LHE G VHKA+KASN+FFD+ G V LSDL VS   K
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMK 180

Query: 181 DENDDDDEYDESSSVPYWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISS 240
                     + SSVPYW P +E+YSFKSDVWS+GILAMEIAYG PPI D ++I++Q S 
Sbjct: 181 ---------KDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSK 240

Query: 241 ISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKK 300
           ISRRFPYDLNL   SG    S+  RKFGD+ K FV SCL KD+F R SA SLL HPFVKK
Sbjct: 241 ISRRFPYDLNLDNRSG---KSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKK 300

Query: 301 GESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGW 360
           GE+SNFLFKSIAKGMVGIG+ F++DK+R++ LMK +      S         K+L+I+GW
Sbjct: 301 GENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGG-----GKVLRITGW 360

Query: 361 DYNNFLFELEPDEESLGKRVRFRGETIIQFREDQE-SKID-HHMPLAASLVERIDDKFEV 420
           DYNNFLFELEPDEES GKRVRFRGET+  ++E ++ +K D   +    S  E+  +  + 
Sbjct: 361 DYNNFLFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQA 420

Query: 421 MDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSEKEKSF 480
           MD+E  A  LSSLL+++D Q+ +V ML+   G+ + ++LEM K+I RLE EL +EKEKSF
Sbjct: 421 MDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSF 480

Query: 481 QLELDYEVLKLKLPDSEDEEDQED 502
           QLEL++E+LKLKLPDS++E++QE+
Sbjct: 481 QLELNFELLKLKLPDSDEEQEQEE 486

BLAST of Lag0016573 vs. ExPASy TrEMBL
Match: A0A5D3C8A9 (Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00250 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 6.1e-126
Identity = 258/511 (50.49%), Postives = 351/511 (68.69%), Query Frame = 0

Query: 22  FPTTAAGYRILSQIGVSDHSVVYMANVNVE----SATVVVAVKVIDINIDIDPVSVTNLN 81
           FPTT+  Y IL+QIG S +S VY+A  + +    S+TV VA+K+I+    I   ++ N  
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLN-T 62

Query: 82  SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQL 141
           SL+L++H N++ PHCCF ADD RLWIVMP++ AGPLQ++LS+ FP G+PDQF +IL  Q+
Sbjct: 63  SLTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQV 122

Query: 142 LRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLS--DLSVSSSTKDENDDDDEYDESSSVP 201
           L+ LV LH MGFVHKAIK SN+FFDSQG+VKLS  DL +S+        D++ D  SS+P
Sbjct: 123 LQALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISA--------DNKIDRDSSIP 182

Query: 202 YWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGKTSG 261
           YWVP D+EYSFKSD+W +G+LAME+ +GGPPI D RI+ SQ+ +ISRRFPY+L   KT  
Sbjct: 183 YWVPPDDEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYEL---KTET 242

Query: 262 DSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGE-SSNFLFKSIAKGM 321
              +S   R FGD+ K+FV +CL+KD+  RP+A+SLL HPFV KGE ++NFLFKSI KG+
Sbjct: 243 GRRNSGDRRIFGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGV 302

Query: 322 VGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYNNFLFELEPDEES 381
           + I  +FQ +KER++G+M   +   N S        +KI KISGW YN+FLFELEPD ES
Sbjct: 303 IEIDRRFQLEKERIIGIMNNDEGLKNQS--------EKITKISGWVYNDFLFELEPDGES 362

Query: 382 LGKRVRFRGETIIQF----REDQESKIDHHMPLAASLVERID------------------ 441
           LGKRVRF GETI +F     E++E +    +    SL+E I+                  
Sbjct: 363 LGKRVRFGGETIREFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKK 422

Query: 442 DKFEVMDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSE 501
            K +++D E+  K L+S+L ++D QR +V MLI   GY ++++ EM+ EI RLE EL +E
Sbjct: 423 KKKKIVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAE 482

Query: 502 KEKSFQLELDYEVLKLKLPDSEDEEDQEDNS 504
           KE+ FQLEL+YE LKLK+PDS+D++ +  +S
Sbjct: 483 KEQYFQLELEYEFLKLKVPDSDDDQKEYPSS 493

BLAST of Lag0016573 vs. ExPASy TrEMBL
Match: A0A1S3CN88 (serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC103502862 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 6.1e-126
Identity = 258/511 (50.49%), Postives = 351/511 (68.69%), Query Frame = 0

Query: 22  FPTTAAGYRILSQIGVSDHSVVYMANVNVE----SATVVVAVKVIDINIDIDPVSVTNLN 81
           FPTT+  Y IL+QIG S +S VY+A  + +    S+TV VA+K+I+    I   ++ N  
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLN-T 62

Query: 82  SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQL 141
           SL+L++H N++ PHCCF ADD RLWIVMP++ AGPLQ++LS+ FP G+PDQF +IL  Q+
Sbjct: 63  SLTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQV 122

Query: 142 LRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLS--DLSVSSSTKDENDDDDEYDESSSVP 201
           L+ LV LH MGFVHKAIK SN+FFDSQG+VKLS  DL +S+        D++ D  SS+P
Sbjct: 123 LQALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISA--------DNKIDRDSSIP 182

Query: 202 YWVPVDEEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGKTSG 261
           YWVP D+EYSFKSD+W +G+LAME+ +GGPPI D RI+ SQ+ +ISRRFPY+L   KT  
Sbjct: 183 YWVPPDDEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYEL---KTET 242

Query: 262 DSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGE-SSNFLFKSIAKGM 321
              +S   R FGD+ K+FV +CL+KD+  RP+A+SLL HPFV KGE ++NFLFKSI KG+
Sbjct: 243 GRRNSGDRRIFGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGV 302

Query: 322 VGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYNNFLFELEPDEES 381
           + I  +FQ +KER++G+M   +   N S        +KI KISGW YN+FLFELEPD ES
Sbjct: 303 IEIDRRFQLEKERIIGIMNNDEGLKNQS--------EKITKISGWVYNDFLFELEPDGES 362

Query: 382 LGKRVRFRGETIIQF----REDQESKIDHHMPLAASLVERID------------------ 441
           LGKRVRF GETI +F     E++E +    +    SL+E I+                  
Sbjct: 363 LGKRVRFGGETIREFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKK 422

Query: 442 DKFEVMDSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQRELEMTKEISRLEAELTSE 501
            K +++D E+  K L+S+L ++D QR +V MLI   GY ++++ EM+ EI RLE EL +E
Sbjct: 423 KKKKIVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAE 482

Query: 502 KEKSFQLELDYEVLKLKLPDSEDEEDQEDNS 504
           KE+ FQLEL+YE LKLK+PDS+D++ +  +S
Sbjct: 483 KEQYFQLELEYEFLKLKVPDSDDDQKEYPSS 493

BLAST of Lag0016573 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 8.8e-53
Identity = 146/490 (29.80%), Postives = 254/490 (51.84%), Query Frame = 0

Query: 18  HNFQFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDIN---IDIDPVSVT 77
           +  +FP  A  Y I+ +IGV   + VY A + +   ++VVA+K ID++    D D +   
Sbjct: 4   NKLEFPLDAEAYEIICKIGVGVSASVYKA-ICIPMNSMVVAIKAIDLDQSRADFDSLR-R 63

Query: 78  NLNSLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILF 137
              ++SL++H N+L+ +C FT  D  LW+VMPF+  G L +I+S+ FP+GLP+  +++  
Sbjct: 64  ETKTMSLLSHPNILNAYCSFTV-DRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFL 123

Query: 138 HQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESS-- 197
            + L  +  LH+ G +H+ IKA N+  DS GSVKL+D  VS+S  +          SS  
Sbjct: 124 KETLNAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLR 183

Query: 198 -----SVPYWVPVD-----EEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISR 257
                  PYW+  +       Y FK+D+WS GI A+E+A+G PP+S    ++S +  I++
Sbjct: 184 LTDIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITK 243

Query: 258 RFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGES 317
           RF +      TSG S     N+KF  +F++ V  CL +D  +RPSA  LL HPF K  + 
Sbjct: 244 RFHFSDYEINTSGSSKKG--NKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKG 303

Query: 318 SNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYN 377
            +F+ K++   +      F   +     ++ K   + +    +++ ++ K  +ISGW++ 
Sbjct: 304 LDFVVKNVLHSLSNAEQMFMESQ-----ILIKSVGDDDEEEEEEDEEIVKNRRISGWNFR 363

Query: 378 NFLFELEP-----DEESLGKRVRFRGETIIQFREDQESKIDHHMPLAASLVERIDDKFEV 437
               +L P     + +S     R   ++  +  +D  +   + + L  S  E  + + EV
Sbjct: 364 EDDLQLSPVFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAKNQEGEV 423

Query: 438 M--DSENFAKALSSLLESVDQQRNVVMMLITQFGYFNQ---RELEMTKEISRLEAELTSE 483
           +  D +   + L  L +S++ QR  V ++I       +   RE E+ + + +L+ EL +E
Sbjct: 424 VGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELLEMVEKLKIELETE 483

BLAST of Lag0016573 vs. TAIR 10
Match: AT5G14720.1 (Protein kinase superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-44
Identity = 112/350 (32.00%), Postives = 188/350 (53.71%), Query Frame = 0

Query: 21  QFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDI---NIDIDPVSVTNLN 80
           +FP  A  Y++  +IG    + V+ A        VVVA+KV+D+   N D+D +    + 
Sbjct: 8   KFPLNAKDYKLYEEIGDGVSATVHRA--LCIPLNVVVAIKVLDLEKCNNDLDGIR-REVQ 67

Query: 81  SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQL 140
           ++SL+NH NVL  HC FT    +LW+VMP++  G    I+ + +P G  +  +A L  + 
Sbjct: 68  TMSLINHPNVLQAHCSFTTGH-QLWVVMPYMAGGSCLHIIKSSYPDGFEEPVIATLLRET 127

Query: 141 LRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESSSVPYW 200
           L+ LV LH  G +H+ +KA N+  DS G+VKL+D  VS+   D  D     +     P W
Sbjct: 128 LKALVYLHAHGHIHRDVKAGNILLDSNGAVKLADFGVSACMFDTGDRQRSRNTFVGTPCW 187

Query: 201 VPVD-----EEYSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLNLGK 260
           +  +       Y FK+DVWS GI A+E+A+G  P S    ++  + ++ +  P  L+  +
Sbjct: 188 MAPEVMQQLHGYDFKADVWSFGITALELAHGHAPFSKYPPMKVLLMTL-QNAPPGLDYER 247

Query: 261 TSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSNFLFKSIAK 320
                     +++F  +FK+ V +CLVKD  +RP++  LL HPF K    +++L K+I  
Sbjct: 248 ----------DKRFSKAFKEMVGTCLVKDPKKRPTSEKLLKHPFFKHARPADYLVKTILN 307

Query: 321 GMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVKVDKILKISGWDYN 363
           G+  +GD++++ K +   L+ + K+      S++    + I  IS W++N
Sbjct: 308 GLPPLGDRYRQIKSKEADLLMQNKSEYEAHLSQQ----EYIRGISAWNFN 338

BLAST of Lag0016573 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 1.0e-37
Identity = 96/316 (30.38%), Postives = 168/316 (53.16%), Query Frame = 0

Query: 56  VVAVKVIDI---NIDIDPVSVTNLNSLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGP 115
           VVA+K++D    N D++ +S     ++ LV+H NVL  HC F +D   LW++MP++  G 
Sbjct: 41  VVAIKILDFERDNCDLNNIS-REAQTMMLVDHPNVLKSHCSFVSDH-NLWVIMPYMSGGS 100

Query: 116 LQAILSNRFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDL 175
              IL   +P G  +  +A +  + L+GL  LH+ G +H+ +KA N+   ++G+VKL D 
Sbjct: 101 CLHILKAAYPDGFEEAIIATILREALKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDF 160

Query: 176 SVSSSTKDENDDDDEYDESSSVPYWVPVD-----EEYSFKSDVWSIGILAMEIAYGGPPI 235
            VS+   D  D     +     P W+  +       Y FK+D+WS GI  +E+A+G  P 
Sbjct: 161 GVSACLFDSGDRQRTRNTFVGTPCWMAPEVMEQLHGYDFKADIWSFGITGLELAHGHAPF 220

Query: 236 SDTRIIRSQISSISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPS 295
           S    ++  + ++ +  P  L+  +          ++KF  SFK  ++SCLVKD  +RPS
Sbjct: 221 SKYPPMKVLLMTL-QNAPPGLDYER----------DKKFSRSFKQMIASCLVKDPSKRPS 280

Query: 296 ASSLLAHPFVKKGESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKN 355
           A  LL H F K+  SS+++ + +  G+  + ++ Q  K +   ++ ++K        K+ 
Sbjct: 281 AKKLLKHSFFKQARSSDYIARKLLDGLPDLVNRVQAIKRKEEDMLAQEK---MADGEKEE 340

Query: 356 VKVDKILK-ISGWDYN 363
           +  ++  + ISGW++N
Sbjct: 341 LSQNEYKRGISGWNFN 340

BLAST of Lag0016573 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 1.0e-37
Identity = 96/316 (30.38%), Postives = 168/316 (53.16%), Query Frame = 0

Query: 56  VVAVKVIDI---NIDIDPVSVTNLNSLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGP 115
           VVA+K++D    N D++ +S     ++ LV+H NVL  HC F +D   LW++MP++  G 
Sbjct: 38  VVAIKILDFERDNCDLNNIS-REAQTMMLVDHPNVLKSHCSFVSDH-NLWVIMPYMSGGS 97

Query: 116 LQAILSNRFPTGLPDQFLAILFHQLLRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDL 175
              IL   +P G  +  +A +  + L+GL  LH+ G +H+ +KA N+   ++G+VKL D 
Sbjct: 98  CLHILKAAYPDGFEEAIIATILREALKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDF 157

Query: 176 SVSSSTKDENDDDDEYDESSSVPYWVPVD-----EEYSFKSDVWSIGILAMEIAYGGPPI 235
            VS+   D  D     +     P W+  +       Y FK+D+WS GI  +E+A+G  P 
Sbjct: 158 GVSACLFDSGDRQRTRNTFVGTPCWMAPEVMEQLHGYDFKADIWSFGITGLELAHGHAPF 217

Query: 236 SDTRIIRSQISSISRRFPYDLNLGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPS 295
           S    ++  + ++ +  P  L+  +          ++KF  SFK  ++SCLVKD  +RPS
Sbjct: 218 SKYPPMKVLLMTL-QNAPPGLDYER----------DKKFSRSFKQMIASCLVKDPSKRPS 277

Query: 296 ASSLLAHPFVKKGESSNFLFKSIAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKN 355
           A  LL H F K+  SS+++ + +  G+  + ++ Q  K +   ++ ++K        K+ 
Sbjct: 278 AKKLLKHSFFKQARSSDYIARKLLDGLPDLVNRVQAIKRKEEDMLAQEK---MADGEKEE 337

Query: 356 VKVDKILK-ISGWDYN 363
           +  ++  + ISGW++N
Sbjct: 338 LSQNEYKRGISGWNFN 337

BLAST of Lag0016573 vs. TAIR 10
Match: AT1G23700.1 (Protein kinase superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-35
Identity = 110/340 (32.35%), Postives = 184/340 (54.12%), Query Frame = 0

Query: 21  QFPTTAAGYRILSQIGVSDHSVVYMANVNVESATVVVAVKVIDI---NIDIDPVSVTNLN 80
           +FP  A  Y IL +IG      VY A   +     +VA+K+ ++     D++ +    ++
Sbjct: 8   RFPLVAKDYEILEEIG----DGVYRARCIL--LDEIVAIKIWNLEKCTNDLETIR-KEVH 67

Query: 81  SLSLVNHANVLSPHCCFTADDGRLWIVMPFIHAGPLQAILSNRFPTGLPDQFLAILFHQL 140
            LSL++H N+L  HC F  D   LWIVMPF+  G    I+ + +P GL +  +AIL  ++
Sbjct: 68  RLSLIDHPNLLRVHCSF-IDSSSLWIVMPFMSCGSSLNIMKSVYPNGLEEPVIAILLREI 127

Query: 141 LRGLVCLHEMGFVHKAIKASNVFFDSQGSVKLSDLSVSSSTKDENDDDDEYDESSSV--P 200
           L+ LV LH +G +H+ +KA NV  DS+G+VKL D  VS+S  D  +      E++ V  P
Sbjct: 128 LKALVYLHGLGHIHRNVKAGNVLVDSEGTVKLGDFEVSASMFDSVERMRTSSENTFVGNP 187

Query: 201 YWVPVDEE------YSFKSDVWSIGILAMEIAYGGPPISDTRIIRSQISSISRRFPYDLN 260
             +  +++      Y FK D+WS G+ A+E+A+G  P   T ++              LN
Sbjct: 188 RRMAPEKDMQQVDGYDFKVDIWSFGMTALELAHGHSP---TTVL-------------PLN 247

Query: 261 LGKTSGDSSSSITNRKFGDSFKDFVSSCLVKDQFRRPSASSLLAHPFVKKGESSNFLFKS 320
           L  +   +    T  KF  SF++ V++CL++D  +RP+AS LL +PF+++  S+ +L  +
Sbjct: 248 LQNSPFPNYEEDT--KFSKSFRELVAACLIEDPEKRPTASQLLEYPFLQQTLSTEYLAST 307

Query: 321 IAKGMVGIGDKFQRDKERLVGLMKKKKNNSNYSSSKKNVK 350
              G+  +G+++++ KE    L+K    N     S++NV+
Sbjct: 308 FLDGLSPLGERYRKLKEEKAKLVKGVDGNKE-KVSQENVE 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138626.18.3e-16261.43serine/threonine-protein kinase BLUS1-like [Momordica charantia][more]
XP_023006000.13.7e-15459.29serine/threonine-protein kinase BLUS1-like [Cucurbita maxima][more]
XP_023548299.11.3e-15157.76serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo][more]
XP_022958864.15.0e-15157.74serine/threonine-protein kinase BLUS1-like [Cucurbita moschata][more]
KAG6574930.15.0e-15157.74Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
O233041.2e-5129.80Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 P... [more]
P978201.4e-2328.42Mitogen-activated protein kinase kinase kinase kinase 4 OS=Mus musculus OX=10090... [more]
Q9UKE51.2e-2225.25TRAF2 and NCK-interacting protein kinase OS=Homo sapiens OX=9606 GN=TNIK PE=1 SV... [more]
P835101.0e-2123.76Traf2 and NCK-interacting protein kinase OS=Mus musculus OX=10090 GN=Tnik PE=1 S... [more]
Q9JM523.3e-2022.63Misshapen-like kinase 1 OS=Mus musculus OX=10090 GN=Mink1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1CA924.0e-16261.43serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1KYZ41.8e-15459.29serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1H6C52.4e-15157.74serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5D3C8A96.1e-12650.49Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CN886.1e-12650.49serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC1035028... [more]
Match NameE-valueIdentityDescription
AT4G14480.18.8e-5329.80Protein kinase superfamily protein [more]
AT5G14720.11.5e-4432.00Protein kinase superfamily protein [more]
AT1G79640.11.0e-3730.38Protein kinase superfamily protein [more]
AT1G79640.21.0e-3730.38Protein kinase superfamily protein [more]
AT1G23700.11.3e-3532.35Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 455..482
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 26..330
e-value: 3.0E-46
score: 159.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..192
NoneNo IPR availablePANTHERPTHR48014:SF7SERINE/THREONINE-PROTEIN KINASE BLUS1coord: 17..498
NoneNo IPR availablePANTHERPTHR48014SERINE/THREONINE-PROTEIN KINASE FRAY2coord: 17..498
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 29..297
e-value: 1.3E-31
score: 109.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 29..297
score: 30.067978
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 26..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016573.1Lag0016573.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity