Lag0016561 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0016561 vs. NCBI nr
Match: XP_022138700.1 (WAT1-related protein At5g07050-like [Momordica charantia])

HSP 1 Score: 600.1 bits (1546), Expect = 1.3e-167
Identity = 306/374 (81.82%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MATNVIGCFGVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVV 60
           MA N +      F+RFKPHIL+  TQ+GYTF+YF+TDASF HGMNP+VHITYRQ+VA VV
Sbjct: 3   MAMNDVCSLRGIFNRFKPHILMAFTQLGYTFMYFITDASFNHGMNPYVHITYRQLVAGVV 62

Query: 61  MFPFAYFLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIAC 120
           MFPFAYFLERK RPRITVALFLEIFVLSLLG SL+L+MYFASLTYTSPTFL SMVNTIAC
Sbjct: 63  MFPFAYFLERKMRPRITVALFLEIFVLSLLGGSLTLSMYFASLTYTSPTFLASMVNTIAC 122

Query: 121 LTFIIAVMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKA 180
           LTFIIAV+LRME V+LQNPRG+AKVLGTL+SLGGVM+MTFYKGPI+RNL HPLIHIQ KA
Sbjct: 123 LTFIIAVVLRMEDVDLQNPRGVAKVLGTLVSLGGVMIMTFYKGPIIRNLWHPLIHIQHKA 182

Query: 181 TNLHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLT 240
           T LHEDWLKGSILTVSSC+SWA+ +IMQAFTLKRYPAQLSLTTWMN +GA Q+GVF +L 
Sbjct: 183 TGLHEDWLKGSILTVSSCISWAIWYIMQAFTLKRYPAQLSLTTWMNIVGAAQSGVFTVLI 242

Query: 241 QHKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILA 300
           QHK G WTVGLNIDLWCIIY GIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILV +LA
Sbjct: 243 QHKAGAWTVGLNIDLWCIIYTGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVAVLA 302

Query: 301 YFVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQLESDSVHETLKQPDLNIGA 360
           YFVFGQKLYMGSIVGGAIVI+GLYLLLWGKEDDQKLQNK  LESDSVHE+ KQP+L   A
Sbjct: 303 YFVFGQKLYMGSIVGGAIVIVGLYLLLWGKEDDQKLQNKPLLESDSVHESSKQPNLQTNA 362

Query: 361 SAA-NKNNVLQDQP 374
           S+A   N+V Q  P
Sbjct: 363 SSAVIANHVFQHHP 376

BLAST of Lag0016561 vs. NCBI nr
Match: KAG6593843.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 600.1 bits (1546), Expect = 1.3e-167
Identity = 300/374 (80.21%), Postives = 340/374 (90.91%), Query Frame = 0

Query: 1   MATNVIGCFGVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVV 60
           MATNV    GVFF+RFKPHIL+I  Q+GYTF+YF TDASFKHGMNPHVHITYRQIVA+V 
Sbjct: 1   MATNV--RLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVG 60

Query: 61  MFPFAYFLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIAC 120
           MFP AYF ERKTRPR+T  LFLEIFVLSLLG+SLSLNM+FASLTY+SPTF+TS +NTIAC
Sbjct: 61  MFPLAYFFERKTRPRMTATLFLEIFVLSLLGMSLSLNMHFASLTYSSPTFVTSSINTIAC 120

Query: 121 LTFIIAVMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKA 180
           LTFIIAVM RMEIV+L+NPRGIAKV+GTL+SLGGVM+MTFYKGPI++NL HP IHIQ KA
Sbjct: 121 LTFIIAVMFRMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIKNLWHPFIHIQHKA 180

Query: 181 TNLHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLT 240
           +NLHE+WLKGS+LTVSSC+SWA+S+IMQAFTLKRYPAQLSL+TWMN +GA Q+G+FA+LT
Sbjct: 181 SNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGMFAMLT 240

Query: 241 QHKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILA 300
           QHKPGVW+VGLNIDLWCIIY+GIVCSAL +YIQLWCTEEKGPVFVTMF+PL  +LV  LA
Sbjct: 241 QHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALA 300

Query: 301 YFVFGQKLYMGSIVGGAIVILGLYLLLWGKE-DDQKLQNKSQLESDSVHETLKQPDLNIG 360
           YFVFGQKLYMGSIVGG IVI+GLY+LLWGKE D+ +LQNKSQLESDSVHET  QPDL IG
Sbjct: 301 YFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEYDEFQLQNKSQLESDSVHETFNQPDLQIG 360

Query: 361 ASAANKNNVLQDQP 374
           AS  + N+VLQ QP
Sbjct: 361 ASPPH-NHVLQHQP 371

BLAST of Lag0016561 vs. NCBI nr
Match: XP_022930405.1 (WAT1-related protein At4g08300-like isoform X1 [Cucurbita moschata] >XP_023514002.1 WAT1-related protein At4g08300-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 598.6 bits (1542), Expect = 3.7e-167
Identity = 301/374 (80.48%), Postives = 338/374 (90.37%), Query Frame = 0

Query: 1   MATNVIGCFGVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVV 60
           MA NV    GVFF+RFKPHIL+I  Q+GYTF+YF TDASFKHGMNPHVHITYRQIVA+V 
Sbjct: 1   MAMNV--RLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVG 60

Query: 61  MFPFAYFLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIAC 120
           MFP AYF ERKTRPR+TV LFLEIFVLSLLG+SLSLNM+FASLTY+SPTF+TS +NTIAC
Sbjct: 61  MFPLAYFFERKTRPRMTVTLFLEIFVLSLLGMSLSLNMHFASLTYSSPTFVTSSINTIAC 120

Query: 121 LTFIIAVMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKA 180
           LTFIIAVM RMEIV+L+NPRGIAKV+GTL+SLGGVM+MTFYKGPI+RNL HP IHIQ KA
Sbjct: 121 LTFIIAVMFRMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKA 180

Query: 181 TNLHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLT 240
           +NLHE+WLKGS+LTVSSC+SWA+S+IMQAFTLKRYPAQLSL+TWMN +GA Q+GVFA+LT
Sbjct: 181 SNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLT 240

Query: 241 QHKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILA 300
           QHKPGVW+VGLNIDLWCIIY+GIVCSAL +YIQLWCTEEKGPVFVTMF+PL  +LV  LA
Sbjct: 241 QHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALA 300

Query: 301 YFVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQ-KLQNKSQLESDSVHETLKQPDLNIG 360
           YFVFGQKLYMGSIVGG IVI+GLY+LLWGKE D+ +LQNKSQLESDSVHET  QPDL I 
Sbjct: 301 YFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQIS 360

Query: 361 ASAANKNNVLQDQP 374
           AS    N+VLQ QP
Sbjct: 361 ASPP-PNHVLQHQP 371

BLAST of Lag0016561 vs. NCBI nr
Match: XP_023000512.1 (WAT1-related protein At4g08300-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 596.3 bits (1536), Expect = 1.8e-166
Identity = 299/374 (79.95%), Postives = 339/374 (90.64%), Query Frame = 0

Query: 1   MATNVIGCFGVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVV 60
           MA NV    GVFF+RFKPHILII  Q+GYTF+YF TDASFKHGMNPHVHITYRQIVA+V 
Sbjct: 1   MAMNV--RLGVFFTRFKPHILIIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVG 60

Query: 61  MFPFAYFLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIAC 120
           MFP AYF ERKTRPR+T  LFLEIFVLSLLG+SLSLNM+FASLTY+SPTF+TS++NTIAC
Sbjct: 61  MFPLAYFFERKTRPRMTGTLFLEIFVLSLLGMSLSLNMHFASLTYSSPTFVTSLINTIAC 120

Query: 121 LTFIIAVMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKA 180
           LTFIIAVM RMEIV+L+NPRGIAKV+GT +SLGGVM+MTFYKGPI+RN  HP IHIQ KA
Sbjct: 121 LTFIIAVMFRMEIVDLKNPRGIAKVVGTSVSLGGVMIMTFYKGPIIRNPWHPFIHIQHKA 180

Query: 181 TNLHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLT 240
           +NLHE+WLKGS+LTVSSC+SWA+S+IMQAFTLKRYPAQ+SL+TWMN +GA Q+GVFA+LT
Sbjct: 181 SNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQISLSTWMNLVGAAQSGVFAVLT 240

Query: 241 QHKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILA 300
           QH+PGVW+VGLNIDLWCIIY+GIVCSALI+YIQLWCTEEKGPVFVTMF+PL  +LV  LA
Sbjct: 241 QHRPGVWSVGLNIDLWCIIYSGIVCSALIVYIQLWCTEEKGPVFVTMFDPLCTVLVAALA 300

Query: 301 YFVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQ-KLQNKSQLESDSVHETLKQPDLNIG 360
           YFVFGQKLYMGSIVGG IVI+GLY+LLWGKE D+ +LQNKSQLESDSVHET +QPDL IG
Sbjct: 301 YFVFGQKLYMGSIVGGGIVIMGLYMLLWGKESDEFELQNKSQLESDSVHETFQQPDLQIG 360

Query: 361 ASAANKNNVLQDQP 374
           AS    N+VLQ QP
Sbjct: 361 ASPPT-NHVLQHQP 371

BLAST of Lag0016561 vs. NCBI nr
Match: XP_038906925.1 (WAT1-related protein At5g07050-like [Benincasa hispida])

HSP 1 Score: 558.9 bits (1439), Expect = 3.3e-155
Identity = 273/330 (82.73%), Postives = 306/330 (92.73%), Query Frame = 0

Query: 11  VFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLER 70
           VFF+RFKPHI II TQ GYTFLYF TDASFKHGMNPHVHITYRQI+A +V+FPFAYFLER
Sbjct: 10  VFFNRFKPHIFIIFTQFGYTFLYFFTDASFKHGMNPHVHITYRQIIAIIVLFPFAYFLER 69

Query: 71  KTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLR 130
           K+RPRITVALFLEIFVLSLLGVSLSLN YFASL YTSPTF++SM+NTI  LTFIIAV+LR
Sbjct: 70  KSRPRITVALFLEIFVLSLLGVSLSLNTYFASLRYTSPTFISSMLNTIPGLTFIIAVILR 129

Query: 131 MEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKATNLHEDWLKG 190
           ME+V+L++P+GIAKV+GTL+SLGGVM+MTFYKGPI+RN+ HP IHIQ +ATN HEDWLKG
Sbjct: 130 MEVVDLKHPKGIAKVMGTLVSLGGVMIMTFYKGPIIRNIWHPFIHIQHRATNFHEDWLKG 189

Query: 191 SILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPGVWTVG 250
           S+LTVSSCLSWA+S++MQAFTLKRYPAQLSLT WMN IGA QAGV+ +LTQHKPGVW+VG
Sbjct: 190 SLLTVSSCLSWAISYVMQAFTLKRYPAQLSLTMWMNLIGAAQAGVYTVLTQHKPGVWSVG 249

Query: 251 LNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFGQKLYM 310
            NIDLW IIYAGIVCS LIIYIQLWCTEEKGPVFVTM+NPL  ILV +LAYFVFGQKLY+
Sbjct: 250 FNIDLWAIIYAGIVCSGLIIYIQLWCTEEKGPVFVTMYNPLCTILVALLAYFVFGQKLYL 309

Query: 311 GSIVGGAIVILGLYLLLWGKEDDQKLQNKS 341
           GSIVGG IVI+GLYLLLWGK+D+QKLQNKS
Sbjct: 310 GSIVGGGIVIIGLYLLLWGKQDEQKLQNKS 339

BLAST of Lag0016561 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 7.0e-68
Identity = 134/333 (40.24%), Postives = 204/333 (61.26%), Query Frame = 0

Query: 15  RFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERKTRP 74
           + KP I IIS Q GY  +Y +T  SFKHGMN  +  TYR +VAT+V+ PFA  LERK RP
Sbjct: 8   KLKPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRP 67

Query: 75  RITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRMEIV 134
           ++T  LFL I  L  L   L  N+Y+  +  TS T+ ++ VN +  +TFI+AV+ R+E V
Sbjct: 68  KMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETV 127

Query: 135 NLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLR--HPLIHIQQKATN---LHEDWLK 194
           NL+  R +AKV+GT I++GG M+MT YKGP +   +  H  +H     T+     ++W+ 
Sbjct: 128 NLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETTDQNWVT 187

Query: 195 GSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPGVWTV 254
           G++  + S  +WA  FI+Q+FTLK+YPA+LSL  W+  +G V   + +L+       W V
Sbjct: 188 GTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKV 247

Query: 255 GLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFGQKLY 314
           G++      +Y+G+VCS +  YIQ     E+GPVF T F+P+  I+   L   V  +K++
Sbjct: 248 GMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIH 307

Query: 315 MGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQL 343
           +GSI+G   ++ GLY ++WGK  D+ +  + ++
Sbjct: 308 LGSIIGAIFIVFGLYSVVWGKAKDEVISVEEKI 340

BLAST of Lag0016561 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.3e-66
Identity = 133/356 (37.36%), Postives = 220/356 (61.80%), Query Frame = 0

Query: 12  FFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERK 71
           F +  KP+  +IS Q GY  +  +T  S   GM+ +V + YR  +AT V+ PFA+F ERK
Sbjct: 12  FLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERK 71

Query: 72  TRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRM 131
            +P+IT ++F+++F+L LLG  +  N Y+  L YTSPTF  +M N +  +TFI+AV+ RM
Sbjct: 72  AQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRM 131

Query: 132 EIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQ---------QKATN 191
           E+++L+     AK+ GT++++ G M+MT YKGPI+       +HIQ          K ++
Sbjct: 132 EMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSS 191

Query: 192 LHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPA-QLSLTTWMNFIGAVQAGVFALLTQ 251
             +++LKGSIL + + L+WA  F++QA  LK Y   QLSLTT + FIG +QA     + +
Sbjct: 192 SDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVME 251

Query: 252 HKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAY 311
           H P  W +G +++L    Y+GIV S++  Y+Q    +++GPVF T F+PL  ++V ++  
Sbjct: 252 HNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGS 311

Query: 312 FVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQLESDSVHETLKQPDLN 358
           FV  +K+++G ++G  ++++GLY +LWGK+ + ++      + DS  +  +  + N
Sbjct: 312 FVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEAN 367

BLAST of Lag0016561 vs. ExPASy Swiss-Prot
Match: Q9LPF1 (WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.9e-66
Identity = 128/327 (39.14%), Postives = 201/327 (61.47%), Query Frame = 0

Query: 10  GVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLE 69
           G    + KP + IIS Q GY  +Y +T  SFKHGM+  V  TYR +VATVVM PFA   E
Sbjct: 3   GGSMEKIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFE 62

Query: 70  RKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVML 129
           RK RP++T+A+F  +  L +L   +  N+Y+  L  TS ++ ++  N +  +TFI+A++ 
Sbjct: 63  RKIRPKMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIF 122

Query: 130 RMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLR--HPLIHIQQKATNLHEDW 189
           R+E VN +    +AKV+GT+I++GG M+MT YKGP +  ++  H   H    +T   + W
Sbjct: 123 RLETVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSSTPTGQHW 182

Query: 190 LKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPGVW 249
           + G+I  + S  +WA  FI+Q++TLK YPA+LSL T +  IG +   + +L+    P  W
Sbjct: 183 VLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAW 242

Query: 250 TVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFGQK 309
            +G++      +Y+G+VCS +  YIQ    +++GPVF T F+P+  I+   L   V  +K
Sbjct: 243 KIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEK 302

Query: 310 LYMGSIVGGAIVILGLYLLLWGKEDDQ 335
           +++GSI+G   ++LGLY ++WGK  D+
Sbjct: 303 IHLGSIIGAVFIVLGLYSVVWGKSKDE 329

BLAST of Lag0016561 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.3e-65
Identity = 128/338 (37.87%), Postives = 206/338 (60.95%), Query Frame = 0

Query: 16  FKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERKTRPR 75
           +KP I ++S Q GY  L  +   +   GM+PHV  +YR IVAT+ + PFAYFL+RK RP+
Sbjct: 6   WKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPK 65

Query: 76  ITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRMEIVN 135
           +T+++F +I +L LL  ++  N+Y+  + YTS TF  +M N +    FI+A + R+E VN
Sbjct: 66  MTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVN 125

Query: 136 LQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPL-------IHIQQKATNLHEDWL 195
           ++     AK+LGT++++GG M+MT  KGP++     PL       IH     T + +D  
Sbjct: 126 VKKIHSQAKILGTIVTVGGAMLMTVVKGPLI-----PLPWANPHDIHQDSSNTGVKQDLT 185

Query: 196 KGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQH-KPGVW 255
           KG+ L    C+ WA    +QA TLK YP +LSLT ++ F+G++++ + AL  +   P  W
Sbjct: 186 KGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAW 245

Query: 256 TVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFGQK 315
            + L+  L   +Y G++CS +  Y+Q    + +GPVFVT FNPLS ++V IL   +  + 
Sbjct: 246 AIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEV 305

Query: 316 LYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQLESD 346
           +++G I+G  +++LGLY +LWGK  D+   + S ++ +
Sbjct: 306 MFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKE 338

BLAST of Lag0016561 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.3e-65
Identity = 125/336 (37.20%), Postives = 204/336 (60.71%), Query Frame = 0

Query: 17  KPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERKTRPRI 76
           KP++ +IS Q GY  +Y +T  S KHGMN +V   YR  +AT V+ PFA F ERK RP++
Sbjct: 10  KPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKIRPKM 69

Query: 77  TVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRMEIVNL 136
           T  +FL+I +L  +   L  N+Y+  +TYTS TF ++  N +  +TF++A++ R+E VN 
Sbjct: 70  TFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLESVNF 129

Query: 137 QNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRH-----------PLIHIQQKATNLHE 196
           +  R IAKV+GT+I++ G ++MT YKGPI+  +R               H    A  + +
Sbjct: 130 KKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAAAMDK 189

Query: 197 DWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPG 256
            W+ G+++ +     WA  FI+Q+FTLK+YPA+LSLTT +  +G ++    +L+T     
Sbjct: 190 HWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTVRDLS 249

Query: 257 VWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFG 316
            W +G + +L+   Y+G++CS +  Y+Q     E+GPVFV  FNPL  ++   L   V  
Sbjct: 250 AWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALGVVVLS 309

Query: 317 QKLYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQ 342
           + +++GS++G   +I+GLY ++WGK  D+++ +  +
Sbjct: 310 ESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDE 345

BLAST of Lag0016561 vs. ExPASy TrEMBL
Match: A0A6J1CA61 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111009797 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 6.2e-168
Identity = 306/374 (81.82%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MATNVIGCFGVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVV 60
           MA N +      F+RFKPHIL+  TQ+GYTF+YF+TDASF HGMNP+VHITYRQ+VA VV
Sbjct: 3   MAMNDVCSLRGIFNRFKPHILMAFTQLGYTFMYFITDASFNHGMNPYVHITYRQLVAGVV 62

Query: 61  MFPFAYFLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIAC 120
           MFPFAYFLERK RPRITVALFLEIFVLSLLG SL+L+MYFASLTYTSPTFL SMVNTIAC
Sbjct: 63  MFPFAYFLERKMRPRITVALFLEIFVLSLLGGSLTLSMYFASLTYTSPTFLASMVNTIAC 122

Query: 121 LTFIIAVMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKA 180
           LTFIIAV+LRME V+LQNPRG+AKVLGTL+SLGGVM+MTFYKGPI+RNL HPLIHIQ KA
Sbjct: 123 LTFIIAVVLRMEDVDLQNPRGVAKVLGTLVSLGGVMIMTFYKGPIIRNLWHPLIHIQHKA 182

Query: 181 TNLHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLT 240
           T LHEDWLKGSILTVSSC+SWA+ +IMQAFTLKRYPAQLSLTTWMN +GA Q+GVF +L 
Sbjct: 183 TGLHEDWLKGSILTVSSCISWAIWYIMQAFTLKRYPAQLSLTTWMNIVGAAQSGVFTVLI 242

Query: 241 QHKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILA 300
           QHK G WTVGLNIDLWCIIY GIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILV +LA
Sbjct: 243 QHKAGAWTVGLNIDLWCIIYTGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVAVLA 302

Query: 301 YFVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQLESDSVHETLKQPDLNIGA 360
           YFVFGQKLYMGSIVGGAIVI+GLYLLLWGKEDDQKLQNK  LESDSVHE+ KQP+L   A
Sbjct: 303 YFVFGQKLYMGSIVGGAIVIVGLYLLLWGKEDDQKLQNKPLLESDSVHESSKQPNLQTNA 362

Query: 361 SAA-NKNNVLQDQP 374
           S+A   N+V Q  P
Sbjct: 363 SSAVIANHVFQHHP 376

BLAST of Lag0016561 vs. ExPASy TrEMBL
Match: A0A6J1EWU5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111436862 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.8e-167
Identity = 301/374 (80.48%), Postives = 338/374 (90.37%), Query Frame = 0

Query: 1   MATNVIGCFGVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVV 60
           MA NV    GVFF+RFKPHIL+I  Q+GYTF+YF TDASFKHGMNPHVHITYRQIVA+V 
Sbjct: 1   MAMNV--RLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVG 60

Query: 61  MFPFAYFLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIAC 120
           MFP AYF ERKTRPR+TV LFLEIFVLSLLG+SLSLNM+FASLTY+SPTF+TS +NTIAC
Sbjct: 61  MFPLAYFFERKTRPRMTVTLFLEIFVLSLLGMSLSLNMHFASLTYSSPTFVTSSINTIAC 120

Query: 121 LTFIIAVMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKA 180
           LTFIIAVM RMEIV+L+NPRGIAKV+GTL+SLGGVM+MTFYKGPI+RNL HP IHIQ KA
Sbjct: 121 LTFIIAVMFRMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKA 180

Query: 181 TNLHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLT 240
           +NLHE+WLKGS+LTVSSC+SWA+S+IMQAFTLKRYPAQLSL+TWMN +GA Q+GVFA+LT
Sbjct: 181 SNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLT 240

Query: 241 QHKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILA 300
           QHKPGVW+VGLNIDLWCIIY+GIVCSAL +YIQLWCTEEKGPVFVTMF+PL  +LV  LA
Sbjct: 241 QHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALA 300

Query: 301 YFVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQ-KLQNKSQLESDSVHETLKQPDLNIG 360
           YFVFGQKLYMGSIVGG IVI+GLY+LLWGKE D+ +LQNKSQLESDSVHET  QPDL I 
Sbjct: 301 YFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQIS 360

Query: 361 ASAANKNNVLQDQP 374
           AS    N+VLQ QP
Sbjct: 361 ASPP-PNHVLQHQP 371

BLAST of Lag0016561 vs. ExPASy TrEMBL
Match: A0A6J1KDU8 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111494761 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 8.9e-167
Identity = 299/374 (79.95%), Postives = 339/374 (90.64%), Query Frame = 0

Query: 1   MATNVIGCFGVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVV 60
           MA NV    GVFF+RFKPHILII  Q+GYTF+YF TDASFKHGMNPHVHITYRQIVA+V 
Sbjct: 1   MAMNV--RLGVFFTRFKPHILIIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVG 60

Query: 61  MFPFAYFLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIAC 120
           MFP AYF ERKTRPR+T  LFLEIFVLSLLG+SLSLNM+FASLTY+SPTF+TS++NTIAC
Sbjct: 61  MFPLAYFFERKTRPRMTGTLFLEIFVLSLLGMSLSLNMHFASLTYSSPTFVTSLINTIAC 120

Query: 121 LTFIIAVMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKA 180
           LTFIIAVM RMEIV+L+NPRGIAKV+GT +SLGGVM+MTFYKGPI+RN  HP IHIQ KA
Sbjct: 121 LTFIIAVMFRMEIVDLKNPRGIAKVVGTSVSLGGVMIMTFYKGPIIRNPWHPFIHIQHKA 180

Query: 181 TNLHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLT 240
           +NLHE+WLKGS+LTVSSC+SWA+S+IMQAFTLKRYPAQ+SL+TWMN +GA Q+GVFA+LT
Sbjct: 181 SNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQISLSTWMNLVGAAQSGVFAVLT 240

Query: 241 QHKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILA 300
           QH+PGVW+VGLNIDLWCIIY+GIVCSALI+YIQLWCTEEKGPVFVTMF+PL  +LV  LA
Sbjct: 241 QHRPGVWSVGLNIDLWCIIYSGIVCSALIVYIQLWCTEEKGPVFVTMFDPLCTVLVAALA 300

Query: 301 YFVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQ-KLQNKSQLESDSVHETLKQPDLNIG 360
           YFVFGQKLYMGSIVGG IVI+GLY+LLWGKE D+ +LQNKSQLESDSVHET +QPDL IG
Sbjct: 301 YFVFGQKLYMGSIVGGGIVIMGLYMLLWGKESDEFELQNKSQLESDSVHETFQQPDLQIG 360

Query: 361 ASAANKNNVLQDQP 374
           AS    N+VLQ QP
Sbjct: 361 ASPPT-NHVLQHQP 371

BLAST of Lag0016561 vs. ExPASy TrEMBL
Match: A0A0A0LLJ5 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_2G179220 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 9.0e-151
Identity = 272/354 (76.84%), Postives = 311/354 (87.85%), Query Frame = 0

Query: 8   CF-GVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAY 67
           CF  VFF RFKPHILII TQ GYTFLYF  DASFKHGMNPHVHITYRQ +AT+ + PFAY
Sbjct: 28  CFLQVFFIRFKPHILIIFTQFGYTFLYFFVDASFKHGMNPHVHITYRQTLATITLLPFAY 87

Query: 68  FLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIA 127
           FLERK RPRIT+ALFLEIFVLSLLGV+LS N YFASL YTSPTF+TSM+NTIA LTFIIA
Sbjct: 88  FLERKLRPRITLALFLEIFVLSLLGVTLSTNTYFASLRYTSPTFITSMLNTIAGLTFIIA 147

Query: 128 VMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKAT-NLHE 187
           V++R+E+V   NP+GIAKV+GTL+SLGGV++MTFYKGPI+RN+  PLIHIQ KAT +LHE
Sbjct: 148 VVVRLEVVEFDNPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHE 207

Query: 188 DWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPG 247
           DWLKGS+LTVSSCLSWA+S+I+QAFTLKRYPA LSLTTWMN  GAVQ  V+ + TQHK G
Sbjct: 208 DWLKGSLLTVSSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAG 267

Query: 248 VWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFG 307
           VW +G NIDLW IIYAG++CS++IIYIQLWCTEE+GPVFVTM+NPL +ILV +L+YFV G
Sbjct: 268 VWNIGFNIDLWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLSYFVVG 327

Query: 308 QKLYMGSIVGGAIVILGLYLLLWGKEDD-QKLQNKSQLESDSVHETLKQPDLNI 359
           QKLY+GSIVGG IVI+GLYLLLWGK+DD QKLQNKS LESDSVH+T KQ D  I
Sbjct: 328 QKLYLGSIVGGGIVIIGLYLLLWGKQDDEQKLQNKSPLESDSVHQTSKQSDFQI 381

BLAST of Lag0016561 vs. ExPASy TrEMBL
Match: A0A1S3CCF6 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103499410 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 9.0e-151
Identity = 270/352 (76.70%), Postives = 311/352 (88.35%), Query Frame = 0

Query: 8   CF-GVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAY 67
           CF  VFF+RFKPHILII TQ+GYTFLYF  DASFKHGMNPHVHITYRQI+AT+ + PFAY
Sbjct: 6   CFLQVFFTRFKPHILIIFTQVGYTFLYFFIDASFKHGMNPHVHITYRQILATITLLPFAY 65

Query: 68  FLERKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIA 127
           FLERK RPRIT+AL LEIF LSL+GV+LS+N YFASL YTSPTF+TSM+NT+A LTF+IA
Sbjct: 66  FLERKLRPRITLALLLEIFXLSLMGVTLSINTYFASLKYTSPTFITSMLNTVAGLTFVIA 125

Query: 128 VMLRMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQQKAT-NLHE 187
           VM R+E+V  +NP+GIAKV+GTL+SLGGV++MTFYKGPI++++ HPLIHIQ KAT  LHE
Sbjct: 126 VMFRLEVVEFENPKGIAKVMGTLVSLGGVLIMTFYKGPIIKSVCHPLIHIQHKATYYLHE 185

Query: 188 DWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPG 247
           DWLKGS+LTVSSCLSWA+S+I+QAFTLKRYPA LSLTTWMN  GA Q   + +LTQ K G
Sbjct: 186 DWLKGSLLTVSSCLSWAISYILQAFTLKRYPAPLSLTTWMNMFGAAQTAFYTVLTQRKAG 245

Query: 248 VWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFG 307
           VW +GLNIDLW IIYAGI+CS +IIYIQLWCTEEKGPVFVTM+NPL +ILV +LAYFVFG
Sbjct: 246 VWNLGLNIDLWAIIYAGIMCSGIIIYIQLWCTEEKGPVFVTMYNPLCSILVALLAYFVFG 305

Query: 308 QKLYMGSIVGGAIVILGLYLLLWGK-EDDQKLQNKSQLESDSVHETLKQPDL 357
           QKLY GSIVGG IVI+GLYLLLWGK +DDQKLQNKS LESDS+H+T KQP L
Sbjct: 306 QKLYFGSIVGGVIVIIGLYLLLWGKQDDDQKLQNKSPLESDSLHQTSKQPHL 357

BLAST of Lag0016561 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 259.2 bits (661), Expect = 5.0e-69
Identity = 134/333 (40.24%), Postives = 204/333 (61.26%), Query Frame = 0

Query: 15  RFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERKTRP 74
           + KP I IIS Q GY  +Y +T  SFKHGMN  +  TYR +VAT+V+ PFA  LERK RP
Sbjct: 8   KLKPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRP 67

Query: 75  RITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRMEIV 134
           ++T  LFL I  L  L   L  N+Y+  +  TS T+ ++ VN +  +TFI+AV+ R+E V
Sbjct: 68  KMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETV 127

Query: 135 NLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLR--HPLIHIQQKATN---LHEDWLK 194
           NL+  R +AKV+GT I++GG M+MT YKGP +   +  H  +H     T+     ++W+ 
Sbjct: 128 NLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETTDQNWVT 187

Query: 195 GSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPGVWTV 254
           G++  + S  +WA  FI+Q+FTLK+YPA+LSL  W+  +G V   + +L+       W V
Sbjct: 188 GTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKV 247

Query: 255 GLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFGQKLY 314
           G++      +Y+G+VCS +  YIQ     E+GPVF T F+P+  I+   L   V  +K++
Sbjct: 248 GMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIH 307

Query: 315 MGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQL 343
           +GSI+G   ++ GLY ++WGK  D+ +  + ++
Sbjct: 308 LGSIIGAIFIVFGLYSVVWGKAKDEVISVEEKI 340

BLAST of Lag0016561 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 255.0 bits (650), Expect = 9.4e-68
Identity = 133/356 (37.36%), Postives = 220/356 (61.80%), Query Frame = 0

Query: 12  FFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERK 71
           F +  KP+  +IS Q GY  +  +T  S   GM+ +V + YR  +AT V+ PFA+F ERK
Sbjct: 12  FLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERK 71

Query: 72  TRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRM 131
            +P+IT ++F+++F+L LLG  +  N Y+  L YTSPTF  +M N +  +TFI+AV+ RM
Sbjct: 72  AQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRM 131

Query: 132 EIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPLIHIQ---------QKATN 191
           E+++L+     AK+ GT++++ G M+MT YKGPI+       +HIQ          K ++
Sbjct: 132 EMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSS 191

Query: 192 LHEDWLKGSILTVSSCLSWAMSFIMQAFTLKRYPA-QLSLTTWMNFIGAVQAGVFALLTQ 251
             +++LKGSIL + + L+WA  F++QA  LK Y   QLSLTT + FIG +QA     + +
Sbjct: 192 SDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVME 251

Query: 252 HKPGVWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAY 311
           H P  W +G +++L    Y+GIV S++  Y+Q    +++GPVF T F+PL  ++V ++  
Sbjct: 252 HNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGS 311

Query: 312 FVFGQKLYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQLESDSVHETLKQPDLN 358
           FV  +K+++G ++G  ++++GLY +LWGK+ + ++      + DS  +  +  + N
Sbjct: 312 FVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEAN 367

BLAST of Lag0016561 vs. TAIR 10
Match: AT1G44800.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 253.4 bits (646), Expect = 2.7e-67
Identity = 128/327 (39.14%), Postives = 201/327 (61.47%), Query Frame = 0

Query: 10  GVFFSRFKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLE 69
           G    + KP + IIS Q GY  +Y +T  SFKHGM+  V  TYR +VATVVM PFA   E
Sbjct: 3   GGSMEKIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFE 62

Query: 70  RKTRPRITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVML 129
           RK RP++T+A+F  +  L +L   +  N+Y+  L  TS ++ ++  N +  +TFI+A++ 
Sbjct: 63  RKIRPKMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIF 122

Query: 130 RMEIVNLQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLR--HPLIHIQQKATNLHEDW 189
           R+E VN +    +AKV+GT+I++GG M+MT YKGP +  ++  H   H    +T   + W
Sbjct: 123 RLETVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSSTPTGQHW 182

Query: 190 LKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPGVW 249
           + G+I  + S  +WA  FI+Q++TLK YPA+LSL T +  IG +   + +L+    P  W
Sbjct: 183 VLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAW 242

Query: 250 TVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFGQK 309
            +G++      +Y+G+VCS +  YIQ    +++GPVF T F+P+  I+   L   V  +K
Sbjct: 243 KIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEK 302

Query: 310 LYMGSIVGGAIVILGLYLLLWGKEDDQ 335
           +++GSI+G   ++LGLY ++WGK  D+
Sbjct: 303 IHLGSIIGAVFIVLGLYSVVWGKSKDE 329

BLAST of Lag0016561 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-66
Identity = 128/338 (37.87%), Postives = 206/338 (60.95%), Query Frame = 0

Query: 16  FKPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERKTRPR 75
           +KP I ++S Q GY  L  +   +   GM+PHV  +YR IVAT+ + PFAYFL+RK RP+
Sbjct: 6   WKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPK 65

Query: 76  ITVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRMEIVN 135
           +T+++F +I +L LL  ++  N+Y+  + YTS TF  +M N +    FI+A + R+E VN
Sbjct: 66  MTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVN 125

Query: 136 LQNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRHPL-------IHIQQKATNLHEDWL 195
           ++     AK+LGT++++GG M+MT  KGP++     PL       IH     T + +D  
Sbjct: 126 VKKIHSQAKILGTIVTVGGAMLMTVVKGPLI-----PLPWANPHDIHQDSSNTGVKQDLT 185

Query: 196 KGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQH-KPGVW 255
           KG+ L    C+ WA    +QA TLK YP +LSLT ++ F+G++++ + AL  +   P  W
Sbjct: 186 KGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAW 245

Query: 256 TVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFGQK 315
            + L+  L   +Y G++CS +  Y+Q    + +GPVFVT FNPLS ++V IL   +  + 
Sbjct: 246 AIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEV 305

Query: 316 LYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQLESD 346
           +++G I+G  +++LGLY +LWGK  D+   + S ++ +
Sbjct: 306 MFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKE 338

BLAST of Lag0016561 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.0e-66
Identity = 125/336 (37.20%), Postives = 204/336 (60.71%), Query Frame = 0

Query: 17  KPHILIISTQIGYTFLYFLTDASFKHGMNPHVHITYRQIVATVVMFPFAYFLERKTRPRI 76
           KP++ +IS Q GY  +Y +T  S KHGMN +V   YR  +AT V+ PFA F ERK RP++
Sbjct: 10  KPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKIRPKM 69

Query: 77  TVALFLEIFVLSLLGVSLSLNMYFASLTYTSPTFLTSMVNTIACLTFIIAVMLRMEIVNL 136
           T  +FL+I +L  +   L  N+Y+  +TYTS TF ++  N +  +TF++A++ R+E VN 
Sbjct: 70  TFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLESVNF 129

Query: 137 QNPRGIAKVLGTLISLGGVMMMTFYKGPILRNLRH-----------PLIHIQQKATNLHE 196
           +  R IAKV+GT+I++ G ++MT YKGPI+  +R               H    A  + +
Sbjct: 130 KKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAAAMDK 189

Query: 197 DWLKGSILTVSSCLSWAMSFIMQAFTLKRYPAQLSLTTWMNFIGAVQAGVFALLTQHKPG 256
            W+ G+++ +     WA  FI+Q+FTLK+YPA+LSLTT +  +G ++    +L+T     
Sbjct: 190 HWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTVRDLS 249

Query: 257 VWTVGLNIDLWCIIYAGIVCSALIIYIQLWCTEEKGPVFVTMFNPLSAILVPILAYFVFG 316
            W +G + +L+   Y+G++CS +  Y+Q     E+GPVFV  FNPL  ++   L   V  
Sbjct: 250 AWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALGVVVLS 309

Query: 317 QKLYMGSIVGGAIVILGLYLLLWGKEDDQKLQNKSQ 342
           + +++GS++G   +I+GLY ++WGK  D+++ +  +
Sbjct: 310 ESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDE 345