Lag0016538 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016538
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description11S globulin subunit beta
Locationchr12: 38756284 .. 38758137 (-)
RNA-Seq ExpressionLag0016538
SyntenyLag0016538
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCGCCCTTCTCTTCTTTCCTTCTTATGTCTAGCAGTTTTCATCCATGCCTGCCTCGCTCAGATTGAGCAGATCCCTTGGGAATTCCAAGGCGGCGAACTGAGGCAACAACACCGGTTCCAATCTCCCAGAGCATGTCGTCTCGAGAATCTCCGAGCTCACGAGCCCGTTCGCAGGGCAGAGGCGGAGGCCGGTTTCACCGAAGTGTGGGATGAGAACAACGAGGAGTTCCAGTGCGCCGGAGTCAATATGATCCGCCATACGATCCGGCCGAAAGGTATGCTTCAGCCAGGATACTCTAATGCTCTGCTCCTAAACTCATCTTCGTTGTCCAAGGCTCCGGCATTCGAGGTGTTGCCATTCCCGGCTGTCCAGAGACTTACCAGACCGATATGCGAAGATCGCAATCCGCGGGATCTGCGTTTAGAGACCAACACCAGAAGATCCGCGAGTTCAGAGAGGGAGATCTCCTCATTCTCCCGGCCGGAGTTTCTCACTGGATCTATAACCGAGGACAATCTGATCTCATCTTGATCGTATTCGCTGACACTCGCAATGTCGCCAACCAAATCGATCCCTACCTCAGAGTAAGTTGAACAAAAACAGAGTATAACGATTTAATCACTGATTTGAAAAAGAAGTCGCTGAGATTCTTTGTTGAATTTGTTTCAGAAATTCCATCTCGCCGGAAGGCCAGAGCAGATAGAAAGAGGCGTAGAGGAATGGGAAAGAAGAGGCCGCAGGGGATCCGCCGGCGAGAAATCAGGCAATGTGTTCAGCGGATTTGCAGACGAACTTCTGGAGGAAGCTTTCCAGATCGACAGTGGAATGGTGAGGCGGCTGAAGGGAGAAGACGATGAGAGAGACAGGATCGTGCAAGTCGACGAAGATTTCGAGGTGCTCATGCCGGAGAAAGATGAACAAGAGAGACAAAGAGGAAAGTACATCGAATCAGAATCGGACAATGGCTTAGAAGAAACCATCTGCACACTTCGATTGAAGCACAACATCGGCCGATCTGAACGCGCCGACGTGTTCAACCCTCGCGGCGGCCGCATCTCCACCGCCAACTACCATAACCTCCCTGCCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTTCTCTACAGTGTAAGCCGAACTGAATTTTAACTCTCAAATATTTCCCCTGGTTCTGAAACCCTAACAATGGTAATTTCATCAAATCATCCATTTCAAAAGCCCTAACAATGATTTGATTTCTTCCAATCTCCAATTTCAAAAACCCTAAGAACTTTGATTTCTACGAATCATCCATTTCAAAAACCCTAACACAACAACACTAATTTCTTCAAAACCCTAACAAGGATTCGATTTCTTCAAATCTCTAATTTAAAAAAAAAAAAAACCGCAACAACGCCGAAATCTTCCAAATCTCGAATTTCAACGCAAAGCCTAATATCTTCAAATTTCCACCCGCAGAACGCGCTCGTGGCACCACACTACACAGTGAATAGCCACACAGTGGTGTACGCAACGAGAGGCAGCGCGAGAGTGCAAGTCGTGGACAACTACGGGCAGTCCGTGTTCGACGGCGAGCTCCGGGAAGGACAGGTACTGACGATTCCACAGAACTTCGTGGTGATAAAACGGGCAAGCGACAGAGGATTCGAGTGGATCGCATTCAAGACGAACGACAACGCGGTGACGAACCTGCTGGCGGGGCGAGCTTCGCAAATGAGGTTGTTGCCGTTGGGAGTTCTGTCGAGCTCGTACCGGATCTCGAGGGAGGAGGCGCAGAGGCTCAAGTACGGGCAGCAAGAGATGAGGGTGCTGAGCCCCGGAAGGTCGCAAGGAAGAAGAGACTGA

mRNA sequence

ATGGGTCGCCCTTCTCTTCTTTCCTTCTTATGTCTAGCAGTTTTCATCCATGCCTGCCTCGCTCAGATTGAGCAGATCCCTTGGGAATTCCAAGGCGGCGAACTGAGGCAACAACACCGGTTCCAATCTCCCAGAGCATGTCGTCTCGAGAATCTCCGAGCTCACGAGCCCGTTCGCAGGGCAGAGGCGGAGGCCGGTTTCACCGAAGTGTGGGATGAGAACAACGAGGAGTTCCAGTGCGCCGGAGTCAATATGATCCGCCATACGATCCGGCCGAAAGGCTCCGGCATTCGAGGTGTTGCCATTCCCGGCTGTCCAGAGACTTACCAGACCGATATGCGAAGATCGCAATCCGCGGGATCTGCGTTTAGAGACCAACACCAGAAGATCCGCGAGTTCAGAGAGGGAGATCTCCTCATTCTCCCGGCCGGAGTTTCTCACTGGATCTATAACCGAGGACAATCTGATCTCATCTTGATCGTATTCGCTGACACTCGCAATGTCGCCAACCAAATCGATCCCTACCTCAGAAAATTCCATCTCGCCGGAAGGCCAGAGCAGATAGAAAGAGGCGTAGAGGAATGGGAAAGAAGAGGCCGCAGGGGATCCGCCGGCGAGAAATCAGGCAATGTGTTCAGCGGATTTGCAGACGAACTTCTGGAGGAAGCTTTCCAGATCGACAGTGGAATGGTGAGGCGGCTGAAGGGAGAAGACGATGAGAGAGACAGGATCGTGCAAGTCGACGAAGATTTCGAGGTGCTCATGCCGGAGAAAGATGAACAAGAGAGACAAAGAGGAAAGTACATCGAATCAGAATCGGACAATGGCTTAGAAGAAACCATCTGCACACTTCGATTGAAGCACAACATCGGCCGATCTGAACGCGCCGACGTGTTCAACCCTCGCGGCGGCCGCATCTCCACCGCCAACTACCATAACCTCCCTGCCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTTCTCTACAGTAACGCGCTCGTGGCACCACACTACACAGTGAATAGCCACACAGTGGTGTACGCAACGAGAGGCAGCGCGAGAGTGCAAGTCGTGGACAACTACGGGCAGTCCGTGTTCGACGGCGAGCTCCGGGAAGGACAGGTACTGACGATTCCACAGAACTTCGTGGTGATAAAACGGGCAAGCGACAGAGGATTCGAGTGGATCGCATTCAAGACGAACGACAACGCGGTGACGAACCTGCTGGCGGGGCGAGCTTCGCAAATGAGGTTGTTGCCGTTGGGAGTTCTGTCGAGCTCGTACCGGATCTCGAGGGAGGAGGCGCAGAGGCTCAAGTACGGGCAGCAAGAGATGAGGGTGCTGAGCCCCGGAAGGTCGCAAGGAAGAAGAGACTGA

Coding sequence (CDS)

ATGGGTCGCCCTTCTCTTCTTTCCTTCTTATGTCTAGCAGTTTTCATCCATGCCTGCCTCGCTCAGATTGAGCAGATCCCTTGGGAATTCCAAGGCGGCGAACTGAGGCAACAACACCGGTTCCAATCTCCCAGAGCATGTCGTCTCGAGAATCTCCGAGCTCACGAGCCCGTTCGCAGGGCAGAGGCGGAGGCCGGTTTCACCGAAGTGTGGGATGAGAACAACGAGGAGTTCCAGTGCGCCGGAGTCAATATGATCCGCCATACGATCCGGCCGAAAGGCTCCGGCATTCGAGGTGTTGCCATTCCCGGCTGTCCAGAGACTTACCAGACCGATATGCGAAGATCGCAATCCGCGGGATCTGCGTTTAGAGACCAACACCAGAAGATCCGCGAGTTCAGAGAGGGAGATCTCCTCATTCTCCCGGCCGGAGTTTCTCACTGGATCTATAACCGAGGACAATCTGATCTCATCTTGATCGTATTCGCTGACACTCGCAATGTCGCCAACCAAATCGATCCCTACCTCAGAAAATTCCATCTCGCCGGAAGGCCAGAGCAGATAGAAAGAGGCGTAGAGGAATGGGAAAGAAGAGGCCGCAGGGGATCCGCCGGCGAGAAATCAGGCAATGTGTTCAGCGGATTTGCAGACGAACTTCTGGAGGAAGCTTTCCAGATCGACAGTGGAATGGTGAGGCGGCTGAAGGGAGAAGACGATGAGAGAGACAGGATCGTGCAAGTCGACGAAGATTTCGAGGTGCTCATGCCGGAGAAAGATGAACAAGAGAGACAAAGAGGAAAGTACATCGAATCAGAATCGGACAATGGCTTAGAAGAAACCATCTGCACACTTCGATTGAAGCACAACATCGGCCGATCTGAACGCGCCGACGTGTTCAACCCTCGCGGCGGCCGCATCTCCACCGCCAACTACCATAACCTCCCTGCCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTTCTCTACAGTAACGCGCTCGTGGCACCACACTACACAGTGAATAGCCACACAGTGGTGTACGCAACGAGAGGCAGCGCGAGAGTGCAAGTCGTGGACAACTACGGGCAGTCCGTGTTCGACGGCGAGCTCCGGGAAGGACAGGTACTGACGATTCCACAGAACTTCGTGGTGATAAAACGGGCAAGCGACAGAGGATTCGAGTGGATCGCATTCAAGACGAACGACAACGCGGTGACGAACCTGCTGGCGGGGCGAGCTTCGCAAATGAGGTTGTTGCCGTTGGGAGTTCTGTCGAGCTCGTACCGGATCTCGAGGGAGGAGGCGCAGAGGCTCAAGTACGGGCAGCAAGAGATGAGGGTGCTGAGCCCCGGAAGGTCGCAAGGAAGAAGAGACTGA

Protein sequence

MGRPSLLSFLCLAVFIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVRRAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPKGSGIRGVAIPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLEEAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD
Homology
BLAST of Lag0016538 vs. NCBI nr
Match: XP_022964523.1 (11S globulin subunit beta [Cucurbita moschata])

HSP 1 Score: 804.3 bits (2076), Expect = 5.5e-229
Identity = 402/480 (83.75%), Postives = 436/480 (90.83%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAV I+ CL+QIE Q PWEFQG E+ QQHR+QSPRACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVLINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEAGFTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAFRDQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE+REGQVL IPQNFV IKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVREGQVLMIPQNFVAIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lag0016538 vs. NCBI nr
Match: XP_023515280.1 (11S globulin subunit beta [Cucurbita pepo subsp. pepo] >KAG7026167.1 hypothetical protein SDJN02_12666, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 803.5 bits (2074), Expect = 9.4e-229
Identity = 402/480 (83.75%), Postives = 436/480 (90.83%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAVFI+ CL+QIE Q PWEFQG E  QQHR+QSPRACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEEWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEAGFTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAFRDQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE++EGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVQEGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRV SPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVFSPGRSQGRRE 480

BLAST of Lag0016538 vs. NCBI nr
Match: XP_023000493.1 (11S globulin subunit beta [Cucurbita maxima])

HSP 1 Score: 802.0 bits (2070), Expect = 2.7e-228
Identity = 400/480 (83.33%), Postives = 437/480 (91.04%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QSPRACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEAGFTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAF+DQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSERADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lag0016538 vs. NCBI nr
Match: KAG6593835.1 (hypothetical protein SDJN03_13311, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 795.8 bits (2054), Expect = 2.0e-226
Identity = 398/474 (83.97%), Postives = 430/474 (90.72%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAVFI+ CL+QIE Q PWEFQG E  QQHR+QSPRACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEEWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEAGFTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAFRDQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGR 454
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRV SPGR
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVFSPGR 474

BLAST of Lag0016538 vs. NCBI nr
Match: P13744.1 (RecName: Full=11S globulin subunit beta; Contains: RecName: Full=11S globulin gamma chain; AltName: Full=11S globulin acidic chain; Contains: RecName: Full=11S globulin delta chain; AltName: Full=11S globulin basic chain; Flags: Precursor [Cucurbita maxima] >pir|FWPU1B| 11S globulin beta subunit precursor - cucurbita cv. Kurokawa Amakuri [Cucurbita hybrid cultivar] >AAA33110.1 11-S globulin beta-subunit precursor [Cucurbita pepo])

HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 398/480 (82.92%), Postives = 435/480 (90.62%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QSPRACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEA FTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAIFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAF+DQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRS RADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSVRADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lag0016538 vs. ExPASy Swiss-Prot
Match: P13744 (11S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 3.3e-229
Identity = 398/480 (82.92%), Postives = 435/480 (90.62%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QSPRACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEA FTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAIFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAF+DQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRS RADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSVRADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lag0016538 vs. ExPASy Swiss-Prot
Match: A0A1L6K371 (11S globulin OS=Juglans nigra OX=16719 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.5e-115
Identity = 244/513 (47.56%), Postives = 313/513 (61.01%), Query Frame = 0

Query: 1   MGRPSLLSF-LCLAVFIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M +P LLS  LCL   ++ CLAQ         GG  RQQ RF     C+L+ L A EP  
Sbjct: 1   MAKPILLSISLCLVALVNGCLAQ--------SGG--RQQPRFGE---CKLKRLVALEPSN 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRP------------------KGSGIRGVA 120
           R EAEAG  E WD NN++FQCAGV ++R TI P                  KG GI GV 
Sbjct: 61  RIEAEAGVIESWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLLYIVKGRGITGVL 120

Query: 121 IPGCPETY------QTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQS 180
            PGCPET+      Q+ +R S  + S  RD+HQKIR FREGD++  PAGV+HW YN G +
Sbjct: 121 FPGCPETFEESQQGQSRIRPSLRSASFQRDRHQKIRHFREGDVIAFPAGVAHWCYNDGDT 180

Query: 181 DLILIVFADTRNVANQIDPYLRKFHLAGRP------------EQIERGVEEWERRGRRGS 240
            ++ +   DT N ANQ+D   R F+LAG P            EQ  R  +  +R G  G 
Sbjct: 181 PVVAVALMDTTNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRHGEPGQ 240

Query: 241 AGEKSG-NVFSGFADELLEEAFQIDSGMVRRLKGEDDERDRIVQVD-EDFEVLMP--EKD 300
               SG NVFSGF  + L +AF +D+   RRL+ E+D R  IV+V+    +V+ P   ++
Sbjct: 241 QQRGSGNNVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSRE 300

Query: 301 EQERQRGKYIESE---------------SDNGLEETICTLRLKHNIGRSERADVFNPRGG 360
           EQER+  K  E E                DNGLEETICTLRL+ NIG   RAD++    G
Sbjct: 301 EQEREERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAG 360

Query: 361 RISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQ 420
           RISTAN H LP LR ++LSAERG LYS+AL  PH+ +N+H+VVYA RG A VQVVDN+GQ
Sbjct: 361 RISTANSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQ 420

Query: 421 SVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGV 458
           +VFD ELREGQ+LTIPQNF V+KRA + GFEW++FKTN+NA+ + LAGR S +R LP  V
Sbjct: 421 TVFDDELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEV 480

BLAST of Lag0016538 vs. ExPASy Swiss-Prot
Match: B5KVH4 (11S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 9.4e-115
Identity = 243/511 (47.55%), Postives = 318/511 (62.23%), Query Frame = 0

Query: 1   MGRPSLLS-FLCLAV--FIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEP 60
           M +P LLS +LCL +    + CLAQ         GG  RQQH+F     C+L  L A EP
Sbjct: 1   MAKPILLSIYLCLIIVALFNGCLAQ--------SGG--RQQHKFGQ---CQLNRLDALEP 60

Query: 61  VRRAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRP------------------KGSGIRG 120
             R EAEAG  E WD N+++ QCAGV ++R TI P                  +G GI G
Sbjct: 61  TNRIEAEAGVIESWDPNHQQLQCAGVAVVRRTIEPNGLLLPHYSNAPQLVYIARGRGITG 120

Query: 121 VAIPGCPETYQTDMRRSQSA--GSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDL 180
           V  PGCPET++   R+SQ        +D+HQKIR FREGD++  PAGV+HW YN G S +
Sbjct: 121 VLFPGCPETFEESQRQSQQGQRREFQQDRHQKIRHFREGDIIAFPAGVAHWCYNDGSSPV 180

Query: 181 ILIVFADTRNVANQIDPYLRKFHLAGRPEQIER--GVEEWER-------RGRRGSAGEK- 240
           + I   DT N ANQ+D   R F+LAG P+   R  G +E+E+       + RRG  GE+ 
Sbjct: 181 VAIFLLDTHNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRRGEHGEQQ 240

Query: 241 ---SGNVFSGFADELLEEAFQIDSGMVRRLKGEDDERDRIVQVD-EDFEVLMP------- 300
                NVFSGF  E L +AF +D+   RRL+ E+D R  IV+V+    +V+ P       
Sbjct: 241 RDLGNNVFSGFDAEFLADAFNVDTETARRLQSENDHRGSIVRVEGRQLQVIRPRWSREEQ 300

Query: 301 -EKDEQERQRGKYIESE---------SDNGLEETICTLRLKHNIGRSERADVFNPRGGRI 360
             ++ +ER+R +  ESE          DNGLEETICTL L+ NIG   RAD++    GRI
Sbjct: 301 EHEERKERERERESESERRQSRRGGRDDNGLEETICTLSLRENIGDPSRADIYTEEAGRI 360

Query: 361 STANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSV 420
           ST N HNLP LR ++LSAERG LYS+AL  PH+ +N+H+VVYA RG A VQVVDN+GQ+V
Sbjct: 361 STVNSHNLPILRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTV 420

Query: 421 FDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLS 458
           FD ELREGQ+LTIPQNF V+KRA D GFEW++FKTN+NA+ + LAGR S +R LP  VL 
Sbjct: 421 FDDELREGQLLTIPQNFAVVKRARDEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLV 480

BLAST of Lag0016538 vs. ExPASy Swiss-Prot
Match: Q2TPW5 (11S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.7e-114
Identity = 244/514 (47.47%), Postives = 320/514 (62.26%), Query Frame = 0

Query: 1   MGRPSLLS---FLCLAVFIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEP 60
           M +P LLS   FL +A+F + CLAQ         GG  RQQ +F     C+L  L A EP
Sbjct: 1   MAKPILLSIYLFLIVALF-NGCLAQ--------SGG--RQQQQFGQ---CQLNRLDALEP 60

Query: 61  VRRAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRP------------------KGSGIRG 120
             R EAEAG  E WD NN++FQCAGV ++R TI P                  +G GI G
Sbjct: 61  TNRIEAEAGVIESWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLVYIARGRGITG 120

Query: 121 VAIPGCPETYQTDMRRSQSAGSA--FRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDL 180
           V  PGCPET++   R+SQ   S    +D+HQKIR FREGD++  PAGV+HW YN G + +
Sbjct: 121 VLFPGCPETFEESQRQSQQGQSREFQQDRHQKIRHFREGDIIAFPAGVAHWSYNDGSNPV 180

Query: 181 ILIVFADTRNVANQIDPYLRKFHLAGRPEQIER--GVEEWE-------RRGRRGSAGEK- 240
           + I   DT N ANQ+D   R F+LAG P+   R  G +E+E       R+ R G  G++ 
Sbjct: 181 VAISLLDTNNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQRQQRPGEHGQQQ 240

Query: 241 ---SGNVFSGFADELLEEAFQIDSGMVRRLKGEDDERDRIVQVD-EDFEVLMP--EKDEQ 300
                NVFSGF  + L +AF +D+   RRL+ E+D R  IV+V+    +V+ P   ++EQ
Sbjct: 241 RGLGNNVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQ 300

Query: 301 ERQRGKYIESE---------------SDNGLEETICTLRLKHNIGRSERADVFNPRGGRI 360
           ER+  K  E E                DNGLEETICTLRL+ NIG   RAD++    GRI
Sbjct: 301 EREERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRI 360

Query: 361 STANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSV 420
           ST N H LP LR ++LSAERG LYS+AL  PH+ +N+H+VVYA RG A VQVVDN+GQ+V
Sbjct: 361 STVNSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTV 420

Query: 421 FDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLS 459
           FD ELREGQ+LTIPQNF V+KRA + GFEW++FKTN+NA+ + LAGR S +R LP  VL+
Sbjct: 421 FDDELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLA 480

BLAST of Lag0016538 vs. ExPASy Swiss-Prot
Match: Q8GZP6 (11S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occidentale OX=171929 PE=1 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 3.1e-110
Identity = 205/477 (42.98%), Postives = 292/477 (61.22%), Query Frame = 0

Query: 10  LCLAVFIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVRRAEAEAGFTE 69
           +C  +  H CLA                +  +Q    C+++ L A EP  R E EAG  E
Sbjct: 3   VCFLILFHGCLA---------------SRQEWQQQDECQIDRLDALEPDNRVEYEAGTVE 62

Query: 70  VWDENNEEFQCAGVNMIRHTIRP------------------KGSGIRGVAIPGCPETYQT 129
            WD N+E+F+CAGV ++RHTI+P                  +G G+ G++ PGCPETYQ 
Sbjct: 63  AWDPNHEQFRCAGVALVRHTIQPNGLLLPQYSNAPQLIYVVQGEGMTGISYPGCPETYQA 122

Query: 130 DMR-RSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIVFADTRNVAN 189
             + R Q     F+D+HQKIR FR GD++ +PAGV+HW YN G S ++ +   D  N  N
Sbjct: 123 PQQGRQQGQSGRFQDRHQKIRRFRRGDIIAIPAGVAHWCYNEGNSPVVTVTLLDVSNSQN 182

Query: 190 QIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLEEAFQIDSGM 249
           Q+D   RKFHLAG P+ + +  ++ + RGR         N+FSGF  ELL EAFQ+D  +
Sbjct: 183 QLDRTPRKFHLAGNPKDVFQQQQQHQSRGR---------NLFSGFDTELLAEAFQVDERL 242

Query: 250 VRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYIESES--------DNGLEETIC 309
           +++LK ED+    +   D++  V+ P + + ER      ESE         DNG+EETIC
Sbjct: 243 IKQLKSEDNRGGIVKVKDDELRVIRPSRSQSERGSESEEESEDEKRRWGQRDNGIEETIC 302

Query: 310 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVN 369
           T+RLK NI    RAD++ P  GR++T N  NLP L+ ++LS E+GVLY NALV PH+ +N
Sbjct: 303 TMRLKENINDPARADIYTPEVGRLTTLNSLNLPILKWLQLSVEKGVLYKNALVLPHWNLN 362

Query: 370 SHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTN 429
           SH+++Y  +G  +VQVVDN+G  VFDGE+REGQ+L +PQNF V+KRA +  FEWI+FKTN
Sbjct: 363 SHSIIYGCKGKGQVQVVDNFGNRVFDGEVREGQMLVVPQNFAVVKRAREERFEWISFKTN 422

Query: 430 DNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           D A+T+ LAGR S +  +P  VL+++++ISRE+A+++K+  Q+  + S   S   RD
Sbjct: 423 DRAMTSPLAGRTSVLGGMPEEVLANAFQISREDARKIKFNNQQTTLTSGESSHHMRD 455

BLAST of Lag0016538 vs. ExPASy TrEMBL
Match: A0A6J1HL28 (11S globulin subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111464525 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.7e-229
Identity = 402/480 (83.75%), Postives = 436/480 (90.83%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAV I+ CL+QIE Q PWEFQG E+ QQHR+QSPRACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVLINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEAGFTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAFRDQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE+REGQVL IPQNFV IKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVREGQVLMIPQNFVAIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lag0016538 vs. ExPASy TrEMBL
Match: A0A6J1KMS5 (11S globulin subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111494748 PE=3 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.3e-228
Identity = 400/480 (83.33%), Postives = 437/480 (91.04%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIE-QIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVR 60
           M R SL +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QSPRACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGVA 120
           RAEAEAGFTEVWD++N+EFQCAGVNMIRHTIRPK                  G GIRG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRSQSAGSAF+DQHQKIR FREGDLL++PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+GEKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYI--ESESDNGLEE 300
           EAFQID G+VR+LKGEDDERDRIVQVDEDFEVL+PEKDE+ER RG+YI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSERADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lag0016538 vs. ExPASy TrEMBL
Match: A0A6J1KVP5 (11S globulin subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111499130 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.7e-223
Identity = 395/477 (82.81%), Postives = 424/477 (88.89%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVRR 60
           M R SLL   CL VFI+ C AQ E  P EFQGGE  QQHRF SPRACRLENLRA EPVRR
Sbjct: 63  MARSSLLGLSCLLVFINVCFAQFEHFPQEFQGGEAMQQHRFHSPRACRLENLRAQEPVRR 122

Query: 61  AEAEAGFTEVWDENNEEFQCAGVNMIRHTIR------------PK------GSGIRGVAI 120
           AEAEAG TEVWD+ +EEFQCAGVNM+RH IR            PK      G+GIRGVA+
Sbjct: 123 AEAEAGSTEVWDQYSEEFQCAGVNMLRHKIRSRGLLLPGFSNAPKLIFVVQGTGIRGVAL 182

Query: 121 PGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIVF 180
           PGC ETYQTD+RRSQSAGSAFRDQHQKIR+FREGDLL++PAGVSHWIYNRGQSDLILIVF
Sbjct: 183 PGCAETYQTDLRRSQSAGSAFRDQHQKIRQFREGDLLVVPAGVSHWIYNRGQSDLILIVF 242

Query: 181 ADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLEE 240
            DTRNVANQIDPYLRKF LAGRPE +ERG EE E R RRGS GEKSGN+FSGFADELLEE
Sbjct: 243 TDTRNVANQIDPYLRKFFLAGRPEMVERGAEEMEGRSRRGSVGEKSGNMFSGFADELLEE 302

Query: 241 AFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYIESESDNGLEETIC 300
           AF++DSG+VRRLKGEDDERDRIVQVDEDF+VL+PE+DEQER RG+YIESES+NG EETIC
Sbjct: 303 AFKVDSGLVRRLKGEDDERDRIVQVDEDFDVLLPERDEQERSRGRYIESESENGFEETIC 362

Query: 301 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVN 360
           TLRLKHNIGRSERADVFNPRGGRISTANYHNLP LRQVRLSAERGVLYSNALVAPHYTVN
Sbjct: 363 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPFLRQVRLSAERGVLYSNALVAPHYTVN 422

Query: 361 SHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTN 420
           SHTVVYATRGSARVQVVD+ GQSVF+GELREGQVL IPQNFVV+K+ASD+GFEWIAFKTN
Sbjct: 423 SHTVVYATRGSARVQVVDDSGQSVFNGELREGQVLMIPQNFVVMKQASDKGFEWIAFKTN 482

Query: 421 DNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           DNA+TNLLAGRASQMR+ PLGVLS+ YRISREEAQRLKYGQQEMR+LSPGRSQGRRD
Sbjct: 483 DNAITNLLAGRASQMRMFPLGVLSNMYRISREEAQRLKYGQQEMRILSPGRSQGRRD 539

BLAST of Lag0016538 vs. ExPASy TrEMBL
Match: A0A6J1H609 (11S globulin subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111460434 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.3e-220
Identity = 392/477 (82.18%), Postives = 422/477 (88.47%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVRR 60
           M R SLL   CL VFI+ C AQ    P EFQGGE  QQHRF SPRACRLENLRA EPVRR
Sbjct: 1   MARSSLLGLSCLLVFINVCFAQFGHFPQEFQGGEAMQQHRFHSPRACRLENLRAQEPVRR 60

Query: 61  AEAEAGFTEVWDENNEEFQCAGVNMIRHTIR------------PK------GSGIRGVAI 120
           AEAEAG TEVWD+ +EEFQCAGVNM+RH IR            PK      G+GIRGVAI
Sbjct: 61  AEAEAGSTEVWDQYSEEFQCAGVNMLRHKIRSRGLLLPGFSNAPKLIFVVQGTGIRGVAI 120

Query: 121 PGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILIVF 180
           PGC ETYQTD+RRSQSAGSAFRDQHQKIR+FREGDLL++PAGVSHWIYNRGQSDLILIVF
Sbjct: 121 PGCAETYQTDLRRSQSAGSAFRDQHQKIRQFREGDLLVVPAGVSHWIYNRGQSDLILIVF 180

Query: 181 ADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELLEE 240
            DTRNVANQIDPYLRKF LAGRPE +ERG EE E R RRGS GEKSGN+FSGFADELLEE
Sbjct: 181 TDTRNVANQIDPYLRKFFLAGRPEMVERGAEEMEGRSRRGSVGEKSGNMFSGFADELLEE 240

Query: 241 AFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYIESESDNGLEETIC 300
           AF+I SG+VRRLKGEDDERDRIVQVDEDF+VL+PEKDEQER RG+YIESES+NG EETIC
Sbjct: 241 AFKIGSGLVRRLKGEDDERDRIVQVDEDFDVLLPEKDEQERSRGRYIESESENGFEETIC 300

Query: 301 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVN 360
           TLRLKHNIGRSERADVFNPRGGRISTANYHNLP LRQVRLSAERGVLYSNALVAPHYTVN
Sbjct: 301 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPFLRQVRLSAERGVLYSNALVAPHYTVN 360

Query: 361 SHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTN 420
           SHTV+YATRGSARVQVVD+ GQSVF+GE+R+GQVL IPQNFVV+K+ASD+GFEWIAFKTN
Sbjct: 361 SHTVLYATRGSARVQVVDDLGQSVFNGEVRKGQVLMIPQNFVVMKQASDKGFEWIAFKTN 420

Query: 421 DNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           DNA+TNLLAGRAS+MR+LPLGVLS+ YRISREEAQRLKYGQQEMR+LSPG SQGRRD
Sbjct: 421 DNAITNLLAGRASEMRMLPLGVLSNMYRISREEAQRLKYGQQEMRILSPGMSQGRRD 477

BLAST of Lag0016538 vs. ExPASy TrEMBL
Match: A0A1S3CIE1 (11S globulin subunit beta-like OS=Cucumis melo OX=3656 GN=LOC103500763 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.3e-211
Identity = 372/479 (77.66%), Postives = 414/479 (86.43%), Query Frame = 0

Query: 1   MGRPSLLSFLCLAVFIHACLAQIEQIPWE--FQGGELRQQHRFQSPRACRLENLRAHEPV 60
           M R SLL+FLCLAVFI+ CL+Q +Q PW   FQG E RQQHRF SP+AC LENLRA EP 
Sbjct: 1   MARSSLLAFLCLAVFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPA 60

Query: 61  RRAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK------------------GSGIRGV 120
           RR EAEAG+TE+W+ NNEEFQCAGVNM+RHTIRPK                  G+GIRGV
Sbjct: 61  RRIEAEAGYTELWEPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGAGIRGV 120

Query: 121 AIPGCPETYQTDMRRSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILI 180
           A+PGCPETY+TD+RRSQS+G+ FRDQHQKIR FREGDLL+LPAGVS WIYNRGQSDLIL+
Sbjct: 121 AMPGCPETYETDLRRSQSSGN-FRDQHQKIRHFREGDLLVLPAGVSQWIYNRGQSDLILV 180

Query: 181 VFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELL 240
           VF DTRNVANQIDP+ RKF+LAGRPE + RG  E E R  R S GEKSGNVFSGFADE L
Sbjct: 181 VFVDTRNVANQIDPFARKFYLAGRPEMMRRG--EREGRSFRESTGEKSGNVFSGFADEFL 240

Query: 241 EEAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKDEQERQRGKYIESESDNGLEET 300
           EEAFQ+DSG+VRRLKGE+D+RDRIV  +EDF+VLMPE+D+QER RG+Y+E ES+NG EET
Sbjct: 241 EEAFQVDSGLVRRLKGENDDRDRIVFTEEDFDVLMPERDDQERSRGRYVEKESENGFEET 300

Query: 301 ICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYT 360
           +CTLRLKHNIGRSE ADVFNPRGGRISTAN++NLP LRQVRLSAERGVLYSNA+ APHYT
Sbjct: 301 VCTLRLKHNIGRSEHADVFNPRGGRISTANFNNLPFLRQVRLSAERGVLYSNAISAPHYT 360

Query: 361 VNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFK 420
           VN+HTV YATRGSARVQVVDNYGQSVFDGE+REGQVL IPQNFVV+ RASDRGFEWIAFK
Sbjct: 361 VNAHTVAYATRGSARVQVVDNYGQSVFDGEVREGQVLVIPQNFVVMTRASDRGFEWIAFK 420

Query: 421 TNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 460
           TNDNA+TNLLAGR SQMRLLPLGVLS+ YRISREEAQRLKYGQ EMR+ SPGRSQGRR+
Sbjct: 421 TNDNAITNLLAGRVSQMRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQGRRE 476

BLAST of Lag0016538 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 315.8 bits (808), Expect = 5.5e-86
Identity = 177/449 (39.42%), Postives = 247/449 (55.01%), Query Frame = 0

Query: 30  FQGGELRQQHRFQSPRACRLENLRAHEPVRRAEAEAGFTEVWDENNEEFQCAGVNMIRHT 89
           F G E RQ+     P AC    + +  P +  + EAG  EVWD  + E +CAGV + R T
Sbjct: 20  FHGAEARQREA-PFPNACHFSQINSLAPAQATKFEAGQMEVWDHMSPELRCAGVTVARIT 79

Query: 90  IRP------------------KGSGIRGVAIPGCPETYQTDMRRSQSAGSA-----FRDQ 149
           ++P                  +G G+ G    GCPET+      S   G       F D 
Sbjct: 80  LQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDM 139

Query: 150 HQKIREFREGDLLILPAGVSHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPE 209
           HQK+  FR GD+    AGVS W YNRG SD ++++  D  N  NQ+D   R F LAG   
Sbjct: 140 HQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIVIVLDVTNRENQLDQVPRMFQLAGSRT 199

Query: 210 QIERGVEEWERRGRRGSAGEKSGNVFSGFADELLEEAFQIDSGMVRRLKGEDDERDRIVQ 269
           Q E     W              N FSGF   ++ EAF+I+    ++L+ + D R  I++
Sbjct: 200 QEEEQPLTW----------PSGNNAFSGFDPNIIAEAFKINIETAKQLQNQKDNRGNIIR 259

Query: 270 VDEDFEVLMPEKDEQERQRGKYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRI 329
            +     ++P   E +       +    NG+EET CT ++  NI   ER+D F+ R GRI
Sbjct: 260 ANGPLHFVIPPPREWQ-------QDGIANGIEETYCTAKIHENIDDPERSDHFSTRAGRI 319

Query: 330 STANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSV 389
           ST N  NLP LR VRL+A RG LYS  +V P +T N+HTV+Y T G A++QVVD+ GQSV
Sbjct: 320 STLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTANAHTVLYVTGGQAKIQVVDDNGQSV 379

Query: 390 FDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLS 449
           F+ ++ +GQ++ IPQ F V K A + GFEWI+FKTNDNA  N L+G+ S +R +P+ V+ 
Sbjct: 380 FNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQTSYLRAVPVDVIK 439

Query: 450 SSYRISREEAQRLKYGQQE-MRVLSPGRS 455
           +SY ++ EEA+R+K+ QQE M  ++P  S
Sbjct: 440 ASYGVNEEEAKRIKFSQQETMLSMTPSSS 450

BLAST of Lag0016538 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 301.6 bits (771), Expect = 1.1e-81
Identity = 172/426 (40.38%), Postives = 238/426 (55.87%), Query Frame = 0

Query: 42  QSPRACRLENLRAHEPVRRAEAEAGFTEVWDENNEEFQCAGVNMIRHTIRPK-------- 101
           Q P  C+L+ L A EP +  ++E G  EVWD +  + +C+G    R  I P+        
Sbjct: 25  QWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGLFLPTFL 84

Query: 102 ----------GSGIRGVAIPGCPETYQTD----MRRSQSAGSAFRDQHQKIREFREGDLL 161
                     G G+ G  IPGC ET+         + Q     FRD HQK+   R GD +
Sbjct: 85  NAGKLTFVVHGRGLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTI 144

Query: 162 ILPAGVSHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRG 221
             P+GV+ W YN G   LIL+  AD  +  NQ+D  LR F +AG   Q     +EW    
Sbjct: 145 ATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQ----GQEW---- 204

Query: 222 RRGSAGEKSGNVFSGFADELLEEAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPEKD 281
            +G   +K  N+F+GFA E+L +AF+I+    ++L+ + D R  IV+V+  F V+ P   
Sbjct: 205 LQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRP--- 264

Query: 282 EQERQRGKYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQ 341
              R  G     E  NGLEET+CT+R   N+     ADV+ P  G IST N +NLP LR 
Sbjct: 265 PLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRL 324

Query: 342 VRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTI 401
           +RLSA RG +  NA+V P + VN++  +Y T G A +Q+V++ G+ VFD E+  GQ+L +
Sbjct: 325 LRLSALRGSIRKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVV 384

Query: 402 PQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRL 446
           PQ F V+K A    FEWI FKTN+NA  N LAGR S MR LPL V+++ Y+IS EEA+R+
Sbjct: 385 PQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVITNGYQISPEEAKRV 439

BLAST of Lag0016538 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 2.4e-81
Identity = 177/473 (37.42%), Postives = 262/473 (55.39%), Query Frame = 0

Query: 5   SLLSF-LCLAVFIHACLAQIEQIPWEFQGGELRQQHRFQSPRACRLENLRAHEPVRRAEA 64
           SLLSF L L +  H   AQ        QG     Q   Q P  C+L+ L A EP    ++
Sbjct: 6   SLLSFCLTLLILFHGYAAQ--------QG-----QQGQQFPNECQLDQLNALEPSHVLKS 65

Query: 65  EAGFTEVWDENNEEFQCAGVNMIRHTIR------------------PKGSGIRGVAIPGC 124
           EAG  EVWD +  + +C+GV+  R+ I                    KG G+ G  IPGC
Sbjct: 66  EAGRIEVWDHHAPQLRCSGVSFARYIIESKGLYLPSFFNTAKLSFVAKGRGLMGKVIPGC 125

Query: 125 PETYQTDMR-----RSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRGQSDLILI 184
            ET+Q           Q     FRD HQK+   R GD +    GV+ W YN GQ  L+++
Sbjct: 126 AETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWFYNDGQEPLVIV 185

Query: 185 VFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFSGFADELL 244
              D  +  NQ+D   R F+LAG   Q +  ++  E++ ++        N+F+GF  E++
Sbjct: 186 SVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQK--------NIFNGFGPEVI 245

Query: 245 EEAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMP--------EKDEQERQRGKYIESE 304
            +A +ID    ++L+ +DD R  IV+V   F V+ P        E++E+E + G++    
Sbjct: 246 AQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRHGRH---- 305

Query: 305 SDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSN 364
             NGLEETIC+ R   N+    RADV+ P+ G IST N ++LP LR +RLSA RG +  N
Sbjct: 306 -GNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQN 365

Query: 365 ALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDR 424
           A+V P +  N++ ++Y T G A++Q+V++ G  VFDG++ +GQ++ +PQ F V+KRA+  
Sbjct: 366 AMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVVKRATSN 425

Query: 425 GFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQE 446
            F+W+ FKTN NA  N LAGR S +R LPL V+++ ++IS EEA+R+K+   E
Sbjct: 426 RFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVKFNTLE 452

BLAST of Lag0016538 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-73
Identity = 163/492 (33.13%), Postives = 249/492 (50.61%), Query Frame = 0

Query: 42  QSPRACRLENLRAHEPVRRAEAEAGFTEVWDENNEEFQCAGVNMIRHTIR---------- 101
           Q    C L+NL   +     ++EAG  E WD N+ + +C GV++ R+ I           
Sbjct: 32  QLQNECNLDNLDVLQATETIKSEAGQIEYWDHNHPQLRCVGVSVARYVIEQGGLYLPTFF 91

Query: 102 --PK------GSGIRGVAIPGCPETYQTDM------------------------------ 161
             PK      G+GI G  +PGC ET+                                  
Sbjct: 92  TSPKISYVVQGTGISGRVVPGCAETFMDSQPMQGQQQGQPWQGRQGQQGQPWEGQGQQGQ 151

Query: 162 ----------------------------RRSQSAGSAFRDQHQKIREFREGDLLILPAGV 221
                                        + Q     FRD HQK+   R GD+     G 
Sbjct: 152 QGRQGQPWEGQGQQGQQGRQGQQGQPWEGQGQQGQQGFRDMHQKVEHVRRGDVFANTPGS 211

Query: 222 SHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAG 281
           +HWIYN G+  L++I   D  N  NQ+D   R FHLAG  +Q   G  + +         
Sbjct: 212 AHWIYNSGEQPLVIIALLDIANYQNQLDRNPRVFHLAGNNQQGGFGGSQQQ--------- 271

Query: 282 EKSGNVFSGFADELLEEAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMPE-KDEQERQ 341
           ++  N++SGF  +++ +A +ID  + ++L+ + D R  IV+V   F+V+ P  +   E +
Sbjct: 272 QEQKNLWSGFDAQVIAQALKIDVQLAQQLQNQQDSRGNIVRVKGPFQVVRPPLRQPYESE 331

Query: 342 RGKYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSA 401
             ++  S   NGLEETIC++R   NI    RADV+ P  GR+++ N + LP L  VRLSA
Sbjct: 332 EWRHPRSPQGNGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSA 391

Query: 402 ERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFV 456
            RGVL  NA+V P Y +N++ ++Y T G  R+QVV++ GQ+V D ++++GQ++ IPQ F 
Sbjct: 392 TRGVLQGNAMVLPKYNMNANEILYCTGGQGRIQVVNDNGQNVLDQQVQKGQLVVIPQGFA 451

BLAST of Lag0016538 vs. TAIR 10
Match: AT5G44120.2 (RmlC-like cupins superfamily protein )

HSP 1 Score: 263.1 bits (671), Expect = 4.3e-70
Identity = 141/360 (39.17%), Postives = 214/360 (59.44%), Query Frame = 0

Query: 99  GVAIPGCPETYQTDMR-----RSQSAGSAFRDQHQKIREFREGDLLILPAGVSHWIYNRG 158
           G  IPGC ET+Q           Q     FRD HQK+   R GD +    GV+ W YN G
Sbjct: 2   GKVIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWFYNDG 61

Query: 159 QSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAGEKSGNVFS 218
           Q  L+++   D  +  NQ+D   R F+LAG   Q +  ++  E++ ++        N+F+
Sbjct: 62  QEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQK--------NIFN 121

Query: 219 GFADELLEEAFQIDSGMVRRLKGEDDERDRIVQVDEDFEVLMP--------EKDEQERQR 278
           GF  E++ +A +ID    ++L+ +DD R  IV+V   F V+ P        E++E+E + 
Sbjct: 122 GFGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRH 181

Query: 279 GKYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAE 338
           G++      NGLEETIC+ R   N+    RADV+ P+ G IST N ++LP LR +RLSA 
Sbjct: 182 GRH-----GNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSAL 241

Query: 339 RGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVV 398
           RG +  NA+V P +  N++ ++Y T G A++Q+V++ G  VFDG++ +GQ++ +PQ F V
Sbjct: 242 RGSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSV 301

Query: 399 IKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQE 446
           +KRA+   F+W+ FKTN NA  N LAGR S +R LPL V+++ ++IS EEA+R+K+   E
Sbjct: 302 VKRATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVKFNTLE 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022964523.15.5e-22983.7511S globulin subunit beta [Cucurbita moschata][more]
XP_023515280.19.4e-22983.7511S globulin subunit beta [Cucurbita pepo subsp. pepo] >KAG7026167.1 hypothetica... [more]
XP_023000493.12.7e-22883.3311S globulin subunit beta [Cucurbita maxima][more]
KAG6593835.12.0e-22683.97hypothetical protein SDJN03_13311, partial [Cucurbita argyrosperma subsp. sorori... [more]
P13744.12.6e-22682.92RecName: Full=11S globulin subunit beta; Contains: RecName: Full=11S globulin ga... [more]
Match NameE-valueIdentityDescription
P137443.3e-22982.9211S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
A0A1L6K3711.5e-11547.5611S globulin OS=Juglans nigra OX=16719 PE=1 SV=1[more]
B5KVH49.4e-11547.5511S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1[more]
Q2TPW52.7e-11447.4711S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1[more]
Q8GZP63.1e-11042.9811S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occident... [more]
Match NameE-valueIdentityDescription
A0A6J1HL282.7e-22983.7511S globulin subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111464525 PE=3 SV=... [more]
A0A6J1KMS51.3e-22883.3311S globulin subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111494748 PE=3 SV=1[more]
A0A6J1KVP53.7e-22382.8111S globulin subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111499130 PE=3 ... [more]
A0A6J1H6091.3e-22082.1811S globulin subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111460434 PE=... [more]
A0A1S3CIE14.3e-21177.6611S globulin subunit beta-like OS=Cucumis melo OX=3656 GN=LOC103500763 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G03890.15.5e-8639.42RmlC-like cupins superfamily protein [more]
AT1G03880.11.1e-8140.38cruciferin 2 [more]
AT5G44120.32.4e-8137.42RmlC-like cupins superfamily protein [more]
AT4G28520.11.1e-7333.13cruciferin 3 [more]
AT5G44120.24.3e-7039.17RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR00604411-S seed storage protein, plantPRINTSPR0043911SGLOBULINcoord: 356..372
score: 59.54
coord: 414..431
score: 37.96
coord: 287..304
score: 47.69
coord: 374..389
score: 58.91
coord: 392..410
score: 49.29
coord: 310..330
score: 51.53
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 50..232
e-value: 2.3E-15
score: 67.0
coord: 288..437
e-value: 3.2E-54
score: 196.1
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 294..436
e-value: 1.9E-32
score: 111.9
coord: 92..186
e-value: 5.6E-13
score: 48.7
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 291..459
e-value: 2.1E-73
score: 247.1
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 46..290
e-value: 6.3E-96
score: 322.7
NoneNo IPR availablePANTHERPTHR31189:SF48LEGUMIN Bcoord: 8..454
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 8..454
NoneNo IPR availableCDDcd02242cupin_11S_legumin_Ncoord: 49..249
e-value: 7.57913E-83
score: 252.506
NoneNo IPR availableCDDcd02243cupin_11S_legumin_Ccoord: 297..451
e-value: 3.57268E-85
score: 256.247
IPR02237911-S seed storage protein, conserved sitePROSITEPS0030511S_SEED_STORAGEcoord: 275..297
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 35..445

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016538.1Lag0016538.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0045735 nutrient reservoir activity