Lag0016497 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016497
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionmRNA cap-binding protein
Locationchr12: 38404335 .. 38406246 (+)
RNA-Seq ExpressionLag0016497
SyntenyLag0016497
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGTGAGGTAGCGGTGGAGGGTGCTGCGGCGGCGGCCGCCGAAGTAGCCGATTCGAATCAACAGTCCCACAAATTGGAGAGAAAATGGACTTTTTGGTTCGATAACCAGTCGAGGCCGAAGCAAGGTGCGGCTTGGGGCACCTCTCTGCGCAAGGTCTATACCTTCGACACCGTCGAAGAATTTTGGTGGTACGTTCTTCGATCCCCCTCTCCGCCGATGTTTGAATTTCGGGGTTTTCTTTACCTTTTCTGTTGCGCCAATTTTTGTTTTGGTAGATTAGGGCATGGCTCCTTTGATGGTAATTTTCTCCTACTGTATTTTAGCTGCCGAGAAATCGGGATTTGAAGAGAACTGTTCCATTCATATATGCGTATTTGATTGAATTTTACGTTGTAGGGCTTGTGTTTTCATTTTACCTTTAAGTTGAGATTAAACGCAGTAGACCTGATTTTTTTATTTATAATTTATGATTTCGATTGTTGTGCAGTTTGTATGATCAGATATTCAAGCCAAGCAAGCTGCCGGCTAATGCAGATTTCCATTTGTTCAAATCTGGAGTTGAGCCGAAATGGGAAGATCCTGAGTGCGCTAATGGAGGAAAATGGACTGTCACGAGCAGTAGAAAGGCTAACCTCGATAACATGTGGCTGGAAACGGTATAGAAACCGTAGAATACTATTATCATTTTATGCTGCCAAAGTTTATTGTGATCTAAATCTGCTTCATATTCGTTCTTGGTCAGTTGATGGCTCTAATTGGGGAACAATTTGAGGAGTCTGATGAGATCTGTGGCGTAGTTGCTAGTGTGCGCCAGAGGCAGGACAAACTTGCATTGTGGACCAAGACAGCTACAAATGAGGCTGCTCAGGTTTGGCCTTCTTTCTTATTTAGCTGCTTTTGCACATTTTCATTGATTTTGTATATTAGTGATAGCCTATCTTCTACGAGATTACTGTGTGACACAAGCTTCACACGCTATTTTCTTATCTCCTGGGCTTTGGTTTGATTCTACCTGTCGACTATGTTCCTTTTTGAGATCTTATGTGATGTTCATGAAGTTTTTGAAAGATATTATGTTCTTTGCTTGTATGAAAATCCTCTTGGCTTCTTCTGATGTGTATGGATTACCGTTTGTGTTTTACTCTTCATGTCACACTTCGTTTTGGCTTCTCTAGAATAAAGTTTTGCTCAATTTCGTACATTTGTGCAAAGGATTTGAGTACAGTATTTAATGTCAATGACTCAATGCATCGTTTCATGTTTTTTCTTACAATTTATCTATTTCATTAGATGGCGAGCCATTTGGCACATTTCATTTATAATGTATTGCCTTTATCTTAAATCAAATGCATTTATTAGTCCCTCAATCAGCAAGGGACTAGGGAGGGAACTTAGGCTTTGGGCTTATTCAATTGGTTGCAGAATTAACAGAGAGTTCTACCTGTGGCATTCCTTCTCTCTGTTGTTTTGCAATTCAATTTTTCAACTTATAACAACTTCTTTAGGAAATAGTAACTAACTAGCTCTGCTCCTTTTCTTCAGCATGTTTCAAGTTCTAAGTTCTTTAAGATCTCAGAGATATATATTATATTGAATTTATATTTTATTTTAATCGACCCCATTATGTGTCAAACCACCCCTAAAAGCATCTAAGCTCAATTCTAAAGACAATCGATGACTTAATCTCTTATTTTTTGATGCAGATGAGCATTGGTAGGAAATGGAAGGAGATCATCGACGTCAACGACAAGATTTCCTTCAGCTTTCATGTAAACTTCAGTTTTGTTGTCGTCAACATTTTCACTTCTTTTCTCCCCATTTCTTCTATTAAATCTCTATTTGTTTTGGTTGGTTGCAGGAGGATTTGAGAAGGGAAAAGTCAGCAAAAGCTCGATACAGTGTCTGA

mRNA sequence

ATGGCCGGTGAGGTAGCGGTGGAGGGTGCTGCGGCGGCGGCCGCCGAAGTAGCCGATTCGAATCAACAGTCCCACAAATTGGAGAGAAAATGGACTTTTTGGTTCGATAACCAGTCGAGGCCGAAGCAAGGTGCGGCTTGGGGCACCTCTCTGCGCAAGGTCTATACCTTCGACACCGTCGAAGAATTTTGGTGTTTGTATGATCAGATATTCAAGCCAAGCAAGCTGCCGGCTAATGCAGATTTCCATTTGTTCAAATCTGGAGTTGAGCCGAAATGGGAAGATCCTGAGTGCGCTAATGGAGGAAAATGGACTGTCACGAGCAGTAGAAAGGCTAACCTCGATAACATGTGGCTGGAAACGTTGATGGCTCTAATTGGGGAACAATTTGAGGAGTCTGATGAGATCTGTGGCGTAGTTGCTAGTGTGCGCCAGAGGCAGGACAAACTTGCATTGTGGACCAAGACAGCTACAAATGAGGCTGCTCAGATGAGCATTGGTAGGAAATGGAAGGAGATCATCGACGTCAACGACAAGATTTCCTTCAGCTTTCATGAGGATTTGAGAAGGGAAAAGTCAGCAAAAGCTCGATACAGTGTCTGA

Coding sequence (CDS)

ATGGCCGGTGAGGTAGCGGTGGAGGGTGCTGCGGCGGCGGCCGCCGAAGTAGCCGATTCGAATCAACAGTCCCACAAATTGGAGAGAAAATGGACTTTTTGGTTCGATAACCAGTCGAGGCCGAAGCAAGGTGCGGCTTGGGGCACCTCTCTGCGCAAGGTCTATACCTTCGACACCGTCGAAGAATTTTGGTGTTTGTATGATCAGATATTCAAGCCAAGCAAGCTGCCGGCTAATGCAGATTTCCATTTGTTCAAATCTGGAGTTGAGCCGAAATGGGAAGATCCTGAGTGCGCTAATGGAGGAAAATGGACTGTCACGAGCAGTAGAAAGGCTAACCTCGATAACATGTGGCTGGAAACGTTGATGGCTCTAATTGGGGAACAATTTGAGGAGTCTGATGAGATCTGTGGCGTAGTTGCTAGTGTGCGCCAGAGGCAGGACAAACTTGCATTGTGGACCAAGACAGCTACAAATGAGGCTGCTCAGATGAGCATTGGTAGGAAATGGAAGGAGATCATCGACGTCAACGACAAGATTTCCTTCAGCTTTCATGAGGATTTGAGAAGGGAAAAGTCAGCAAAAGCTCGATACAGTGTCTGA

Protein sequence

MAGEVAVEGAAAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHEDLRREKSAKARYSV
Homology
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BLAST of Lag0016497 vs. NCBI nr
Match: XP_022138462.1 (eukaryotic translation initiation factor [Momordica charantia])

HSP 1 Score: 402.9 bits (1034), Expect = 1.6e-108
Identity = 194/200 (97.00%), Postives = 199/200 (99.50%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTV 60
           MAGEVAVEGAAAAAAEVADSNQQSH+LERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTV
Sbjct: 1   MAGEVAVEGAAAAAAEVADSNQQSHRLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTV 60

Query: 61  EEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLE 120
           EEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLE
Sbjct: 61  EEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLE 120

Query: 121 TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKI 180
           TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDV DKI
Sbjct: 121 TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVTDKI 180

Query: 181 SFSFHEDLRREKSAKARYSV 201
           ++SFHEDLRREKSAK+RY+V
Sbjct: 181 TYSFHEDLRREKSAKSRYNV 200

[Close]
BLAST of Lag0016497 vs. NCBI nr
Match: XP_008460683.1 (PREDICTED: eukaryotic translation initiation factor [Cucumis melo] >ABY56090.1 eukaryotic initiation factor iso4E [Cucumis melo])

HSP 1 Score: 398.7 bits (1023), Expect = 3.0e-107
Identity = 195/203 (96.06%), Postives = 199/203 (98.03%), Query Frame = 0 

Query: 1   MAGEVAVEGA---AAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60
           MAGEVAVEGA   AAAA+EVADSN QSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF
Sbjct: 1   MAGEVAVEGAVAVAAAASEVADSNPQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60

Query: 61  DTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120
           +TVEEFWCLYDQ+FKPSKLPANADFHLFK+GVEPKWEDPECANGGKWTVTSSRKANLDNM
Sbjct: 61  ETVEEFWCLYDQLFKPSKLPANADFHLFKTGVEPKWEDPECANGGKWTVTSSRKANLDNM 120

Query: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180
           WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN
Sbjct: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180

Query: 181 DKISFSFHEDLRREKSAKARYSV 201
           DKISFSFHEDLRREKSAKARYSV
Sbjct: 181 DKISFSFHEDLRREKSAKARYSV 203

[Close]
BLAST of Lag0016497 vs. NCBI nr
Match: XP_038875852.1 (eukaryotic translation initiation factor [Benincasa hispida])

HSP 1 Score: 397.1 bits (1019), Expect = 8.8e-107
Identity = 194/201 (96.52%), Postives = 197/201 (98.01%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAA-AAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDT 60
           MAGEV VEGAAAA A E+ADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDT
Sbjct: 1   MAGEVTVEGAAAAVATELADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDT 60

Query: 61  VEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWL 120
           VEEFWCLYDQ+FKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLD+MWL
Sbjct: 61  VEEFWCLYDQLFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDSMWL 120

Query: 121 ETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDK 180
           ETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDV DK
Sbjct: 121 ETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVTDK 180

Query: 181 ISFSFHEDLRREKSAKARYSV 201
           ISFSFHEDLRREKSAKARYSV
Sbjct: 181 ISFSFHEDLRREKSAKARYSV 201

[Close]
BLAST of Lag0016497 vs. NCBI nr
Match: XP_004147164.1 (eukaryotic translation initiation factor [Cucumis sativus] >ABY56102.1 eukaryotic initiation factor iso4E [Cucumis sativus] >KGN61568.1 hypothetical protein Csa_006416 [Cucumis sativus])

HSP 1 Score: 396.4 bits (1017), Expect = 1.5e-106
Identity = 194/203 (95.57%), Postives = 197/203 (97.04%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAAAA---EVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60
           MAGEVAVEGA AAAA   EVADSN Q+HKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF
Sbjct: 1   MAGEVAVEGAVAAAALATEVADSNPQAHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60

Query: 61  DTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120
           DTVEEFWCLYDQ+FKPSKLPANADFHLFK+GVEPKWEDPECANGGKWTVTSSRKANLDNM
Sbjct: 61  DTVEEFWCLYDQLFKPSKLPANADFHLFKTGVEPKWEDPECANGGKWTVTSSRKANLDNM 120

Query: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180
           WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDV 
Sbjct: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVT 180

Query: 181 DKISFSFHEDLRREKSAKARYSV 201
           DKISFSFHEDLRREKSAKARYSV
Sbjct: 181 DKISFSFHEDLRREKSAKARYSV 203

[Close]
BLAST of Lag0016497 vs. NCBI nr
Match: AJA90794.1 (eukaryotic translation inititation factor (iso)4E [Citrullus lanatus])

HSP 1 Score: 395.6 bits (1015), Expect = 2.6e-106
Identity = 194/203 (95.57%), Postives = 197/203 (97.04%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAAAA---EVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60
           MAGEVAVEGA AAAA   E+ADS+QQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF
Sbjct: 1   MAGEVAVEGAVAAAAVATELADSSQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60

Query: 61  DTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120
           DTVEEFWCLYDQ+FKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM
Sbjct: 61  DTVEEFWCLYDQLFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120

Query: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180
           WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSI RKWKEIIDV 
Sbjct: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSISRKWKEIIDVT 180

Query: 181 DKISFSFHEDLRREKSAKARYSV 201
           DKISFSFHEDLRREKSAKARYSV
Sbjct: 181 DKISFSFHEDLRREKSAKARYSV 203

[Close]
BLAST of Lag0016497 vs. ExPASy Swiss-Prot
Match: Q03389 (Eukaryotic translation initiation factor isoform 4E-2 OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 7.8e-82
Identity = 144/198 (72.73%), Postives = 167/198 (84.34%), Query Frame = 0 

Query: 4   EVAVEGAAAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEF 63
           EVA EG A AA       +  HKL+R+WTFW+D Q++PK GAAWGTSL+K YTFDTVEEF
Sbjct: 17  EVAAEGDAGAA-----EAKGPHKLQRQWTFWYDIQTKPKPGAAWGTSLKKGYTFDTVEEF 76

Query: 64  WCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLETLM 123
           WCLYDQIF+PSKL  +ADFHLFK+GVEPKWEDPECANGGKWTV SSRK NLD MWLET M
Sbjct: 77  WCLYDQIFRPSKLVGSADFHLFKAGVEPKWEDPECANGGKWTVISSRKTNLDTMWLETCM 136

Query: 124 ALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFS 183
           ALIGEQF+ES EICGVVASVRQRQDKL+LWTKTA+NEA Q+ IG+KWKE+ID NDK+ +S
Sbjct: 137 ALIGEQFDESQEICGVVASVRQRQDKLSLWTKTASNEAVQVDIGKKWKEVIDYNDKMVYS 196

Query: 184 FHEDLRREKSAK-ARYSV 201
           FH+D R +K ++  RY+V
Sbjct: 197 FHDDSRSQKPSRGGRYTV 209

[Close]
BLAST of Lag0016497 vs. ExPASy Swiss-Prot
Match: P48600 (Eukaryotic translation initiation factor isoform 4E-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0467600 PE=2 SV=2)

HSP 1 Score: 303.1 bits (775), Expect = 2.3e-81
Identity = 142/196 (72.45%), Postives = 166/196 (84.69%), Query Frame = 0 

Query: 6   AVEGAAAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWC 65
           A E    AAAE A + +  HKL R+W FW+D QS+PK GAAWGTSLRK YTFDTVEEFW 
Sbjct: 11  AAETPEVAAAEGAAAAKAPHKLHRQWAFWYDIQSKPKPGAAWGTSLRKAYTFDTVEEFWG 70

Query: 66  LYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLETLMAL 125
           LYDQIF+PSK+  NADFHLFK+GVEPKWEDPECANGGKWTV  SRK  L+NMWLETLMAL
Sbjct: 71  LYDQIFRPSKVTVNADFHLFKAGVEPKWEDPECANGGKWTVPCSRKTTLENMWLETLMAL 130

Query: 126 IGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFH 185
           IGEQF+ES+EICGVVASVRQR DKLALWT+TA+NEA Q++IG+KWKEI+D NDK+ +SFH
Sbjct: 131 IGEQFDESEEICGVVASVRQRGDKLALWTRTASNEAVQVNIGKKWKEIVDYNDKMVYSFH 190

Query: 186 EDLRREKSAK-ARYSV 201
           +D +REK ++  RY+V
Sbjct: 191 DDAKREKPSRGGRYNV 206

[Close]
BLAST of Lag0016497 vs. ExPASy Swiss-Prot
Match: O81482 (Eukaryotic translation initiation factor isoform 4E-2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 7.6e-77
Identity = 129/191 (67.54%), Postives = 161/191 (84.29%), Query Frame = 0 

Query: 11  AAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQI 70
           AA  A  A   +  HKL R+WTFW+D Q++ K GAAWGTSL+K YTFDTVEEFW +YDQI
Sbjct: 26  AAPEAGAATEAKGPHKLHRQWTFWYDIQTKTKSGAAWGTSLKKAYTFDTVEEFWSMYDQI 85

Query: 71  FKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLETLMALIGEQF 130
           F+PSKL  NADFHLFK+GVEPKWEDPECANGGKWTV  +RKA  + MWLETLMALIGEQF
Sbjct: 86  FRPSKLSGNADFHLFKAGVEPKWEDPECANGGKWTVPCNRKATFETMWLETLMALIGEQF 145

Query: 131 EESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHEDLRR 190
           +E+++ICG+VASVR R DKLALWT+TA+NEA Q++IG+KWK++ID NDKI+++FH+D +R
Sbjct: 146 DETEDICGIVASVRARGDKLALWTRTASNEAVQVNIGKKWKDVIDYNDKITYTFHDDSKR 205

Query: 191 EKSAK-ARYSV 201
           +K ++  RY+V
Sbjct: 206 DKPSRGGRYTV 216

[Close]
BLAST of Lag0016497 vs. ExPASy Swiss-Prot
Match: O04663 (Eukaryotic translation initiation factor isoform 4E OS=Arabidopsis thaliana OX=3702 GN=EIF(ISO)4E PE=1 SV=2)

HSP 1 Score: 266.5 bits (680), Expect = 2.4e-70
Identity = 128/186 (68.82%), Postives = 153/186 (82.26%), Query Frame = 0 

Query: 11  AAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQI 70
           AAA     ++ +Q HKLERKW+FWFDNQS  K+GAAWG SLRK YTFDTVE+FW L++ I
Sbjct: 12  AAAELPATEAEKQPHKLERKWSFWFDNQS--KKGAAWGASLRKAYTFDTVEDFWGLHETI 71

Query: 71  FKPSKLPANADFHLFKSGVEPKWEDPECANGGKWT--VTSSRKANLDNMWLETLMALIGE 130
           F+ SKL ANA+ HLFK+GVEPKWEDPECANGGKWT  VT++RK  LD  WLETLMALIGE
Sbjct: 72  FQTSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETLMALIGE 131

Query: 131 QFEESDEICGVVASVR--QRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHE 190
           QF+E+DEICGVVASVR   +QDKL+LWT+T +NEA  M IG+KWKEI+DV DKI+F+ H+
Sbjct: 132 QFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHD 191

Query: 191 DLRREK 193
           D RR +
Sbjct: 192 DSRRSR 195

[Close]
BLAST of Lag0016497 vs. ExPASy Swiss-Prot
Match: O81481 (Eukaryotic translation initiation factor 4E-1 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 9.3e-59
Identity = 103/184 (55.98%), Postives = 132/184 (71.74%), Query Frame = 0 

Query: 18  ADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQIFKPSKLP 77
           A S   +H LE  WTFWFDN     + AAWG+S+R ++TF TVEEFW LY+ I  PSKL 
Sbjct: 36  APSPPATHPLEHSWTFWFDNPQSKSKQAAWGSSIRPIHTFSTVEEFWGLYNNINHPSKLI 95

Query: 78  ANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLETLMALIGEQFEESDEIC 137
             ADFH FK+ +EPKWEDP CANGGKWT++  R    D  WL TL+A+IGEQF+  DEIC
Sbjct: 96  VGADFHCFKNKIEPKWEDPICANGGKWTISCGR-GKSDTFWLHTLLAMIGEQFDYGDEIC 155

Query: 138 GVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHEDLRR-EKSAKA 197
           G V SVR +Q+++A+WTK A NEAAQ+SIG++WKE++D  D I F  H+D ++ +K  K 
Sbjct: 156 GAVVSVRGKQERIAIWTKNAANEAAQVSIGKQWKELLDYKDSIGFIVHDDAKKMDKGLKE 215

Query: 198 RYSV 201
           RY+V
Sbjct: 216 RYTV 218

[Close]
BLAST of Lag0016497 vs. ExPASy TrEMBL
Match: A0A6J1CB61 (mRNA cap-binding protein OS=Momordica charantia OX=3673 GN=LOC111009629 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 7.8e-109
Identity = 194/200 (97.00%), Postives = 199/200 (99.50%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTV 60
           MAGEVAVEGAAAAAAEVADSNQQSH+LERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTV
Sbjct: 1   MAGEVAVEGAAAAAAEVADSNQQSHRLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTV 60

Query: 61  EEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLE 120
           EEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLE
Sbjct: 61  EEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLE 120

Query: 121 TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKI 180
           TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDV DKI
Sbjct: 121 TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVTDKI 180

Query: 181 SFSFHEDLRREKSAKARYSV 201
           ++SFHEDLRREKSAK+RY+V
Sbjct: 181 TYSFHEDLRREKSAKSRYNV 200

[Close]
BLAST of Lag0016497 vs. ExPASy TrEMBL
Match: B0F816 (mRNA cap-binding protein OS=Cucumis melo OX=3656 GN=eIF(iso)4E PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.5e-107
Identity = 195/203 (96.06%), Postives = 199/203 (98.03%), Query Frame = 0 

Query: 1   MAGEVAVEGA---AAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60
           MAGEVAVEGA   AAAA+EVADSN QSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF
Sbjct: 1   MAGEVAVEGAVAVAAAASEVADSNPQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60

Query: 61  DTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120
           +TVEEFWCLYDQ+FKPSKLPANADFHLFK+GVEPKWEDPECANGGKWTVTSSRKANLDNM
Sbjct: 61  ETVEEFWCLYDQLFKPSKLPANADFHLFKTGVEPKWEDPECANGGKWTVTSSRKANLDNM 120

Query: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180
           WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN
Sbjct: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180

Query: 181 DKISFSFHEDLRREKSAKARYSV 201
           DKISFSFHEDLRREKSAKARYSV
Sbjct: 181 DKISFSFHEDLRREKSAKARYSV 203

[Close]
BLAST of Lag0016497 vs. ExPASy TrEMBL
Match: B0F832 (mRNA cap-binding protein OS=Cucumis sativus OX=3659 GN=eIF(iso)4E PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 7.3e-107
Identity = 194/203 (95.57%), Postives = 197/203 (97.04%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAAAA---EVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60
           MAGEVAVEGA AAAA   EVADSN Q+HKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF
Sbjct: 1   MAGEVAVEGAVAAAALATEVADSNPQAHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60

Query: 61  DTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120
           DTVEEFWCLYDQ+FKPSKLPANADFHLFK+GVEPKWEDPECANGGKWTVTSSRKANLDNM
Sbjct: 61  DTVEEFWCLYDQLFKPSKLPANADFHLFKTGVEPKWEDPECANGGKWTVTSSRKANLDNM 120

Query: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180
           WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDV 
Sbjct: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVT 180

Query: 181 DKISFSFHEDLRREKSAKARYSV 201
           DKISFSFHEDLRREKSAKARYSV
Sbjct: 181 DKISFSFHEDLRREKSAKARYSV 203

[Close]
BLAST of Lag0016497 vs. ExPASy TrEMBL
Match: A0A0D3N942 (mRNA cap-binding protein OS=Citrullus lanatus OX=3654 GN=eIF4E(iso) PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.2e-106
Identity = 194/203 (95.57%), Postives = 197/203 (97.04%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAAAA---EVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60
           MAGEVAVEGA AAAA   E+ADS+QQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF
Sbjct: 1   MAGEVAVEGAVAAAAVATELADSSQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTF 60

Query: 61  DTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120
           DTVEEFWCLYDQ+FKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM
Sbjct: 61  DTVEEFWCLYDQLFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNM 120

Query: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVN 180
           WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSI RKWKEIIDV 
Sbjct: 121 WLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSISRKWKEIIDVT 180

Query: 181 DKISFSFHEDLRREKSAKARYSV 201
           DKISFSFHEDLRREKSAKARYSV
Sbjct: 181 DKISFSFHEDLRREKSAKARYSV 203

[Close]
BLAST of Lag0016497 vs. ExPASy TrEMBL
Match: A0A6J1KKA7 (mRNA cap-binding protein OS=Cucurbita maxima OX=3661 GN=LOC111494789 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.0e-104
Identity = 190/200 (95.00%), Postives = 194/200 (97.00%), Query Frame = 0 

Query: 1   MAGEVAVEGAAAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTV 60
           MAGEVAVEG AAAA EV DSNQQSHKLERKWTFWFDNQSRPKQGAAWG+SLRKVYTFDTV
Sbjct: 1   MAGEVAVEG-AAAATEVPDSNQQSHKLERKWTFWFDNQSRPKQGAAWGSSLRKVYTFDTV 60

Query: 61  EEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLE 120
           EEFWCLYDQI KPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLD+MWLE
Sbjct: 61  EEFWCLYDQIPKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDSMWLE 120

Query: 121 TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKI 180
           TLMALIGEQFEESDEICGVVASVRQRQDKLALWTK ATNEAAQMS+GRKWKEIIDV DKI
Sbjct: 121 TLMALIGEQFEESDEICGVVASVRQRQDKLALWTKNATNEAAQMSLGRKWKEIIDVTDKI 180

Query: 181 SFSFHEDLRREKSAKARYSV 201
           SFSFHEDLRREKSAK+RYSV
Sbjct: 181 SFSFHEDLRREKSAKSRYSV 199

[Close]
BLAST of Lag0016497 vs. TAIR 10
Match: AT5G35620.1 (Eukaryotic initiation factor 4E protein )

HSP 1 Score: 266.5 bits (680), Expect = 1.7e-71
Identity = 128/186 (68.82%), Postives = 153/186 (82.26%), Query Frame = 0 

Query: 11  AAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQI 70
           AAA     ++ +Q HKLERKW+FWFDNQS  K+GAAWG SLRK YTFDTVE+FW L++ I
Sbjct: 12  AAAELPATEAEKQPHKLERKWSFWFDNQS--KKGAAWGASLRKAYTFDTVEDFWGLHETI 71

Query: 71  FKPSKLPANADFHLFKSGVEPKWEDPECANGGKWT--VTSSRKANLDNMWLETLMALIGE 130
           F+ SKL ANA+ HLFK+GVEPKWEDPECANGGKWT  VT++RK  LD  WLETLMALIGE
Sbjct: 72  FQTSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETLMALIGE 131

Query: 131 QFEESDEICGVVASVR--QRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHE 190
           QF+E+DEICGVVASVR   +QDKL+LWT+T +NEA  M IG+KWKEI+DV DKI+F+ H+
Sbjct: 132 QFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHD 191

Query: 191 DLRREK 193
           D RR +
Sbjct: 192 DSRRSR 195

[Close]
BLAST of Lag0016497 vs. TAIR 10
Match: AT5G35620.2 (Eukaryotic initiation factor 4E protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-59
Identity = 110/155 (70.97%), Postives = 129/155 (83.23%), Query Frame = 0 

Query: 11  AAAAAEVADSNQQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQI 70
           AAA     ++ +Q HKLERKW+FWFDNQS  K+GAAWG SLRK YTFDTVE+FW L++ I
Sbjct: 12  AAAELPATEAEKQPHKLERKWSFWFDNQS--KKGAAWGASLRKAYTFDTVEDFWGLHETI 71

Query: 71  FKPSKLPANADFHLFKSGVEPKWEDPECANGGKWT--VTSSRKANLDNMWLETLMALIGE 130
           F+ SKL ANA+ HLFK+GVEPKWEDPECANGGKWT  VT++RK  LD  WLETLMALIGE
Sbjct: 72  FQTSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETLMALIGE 131

Query: 131 QFEESDEICGVVASVR--QRQDKLALWTKTATNEA 162
           QF+E+DEICGVVASVR   +QDKL+LWT+T +NEA
Sbjct: 132 QFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEA 164

[Close]
BLAST of Lag0016497 vs. TAIR 10
Match: AT4G18040.1 (eukaryotic translation initiation factor 4E )

HSP 1 Score: 216.1 bits (549), Expect = 2.6e-56
Identity = 104/205 (50.73%), Postives = 144/205 (70.24%), Query Frame = 0 

Query: 3   GEVAVEGAAAAA---AEVADSNQ----QSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVY 62
           G+ A EG  A       V +S++    +SH LE  WTFWFDN +   +  +WG+SLR V+
Sbjct: 31  GDDAEEGEIAGGEGDGNVDESSKSGVPESHPLEHSWTFWFDNPAVKSKQTSWGSSLRPVF 90

Query: 63  TFDTVEEFWCLYDQIFKPSKLPANADFHLFKSGVEPKWEDPECANGGKWTVTSSRKANLD 122
           TF TVEEFW LY+ +  PSKL   ADF+ FK  +EPKWEDP CANGGKWT+T  ++ + D
Sbjct: 91  TFSTVEEFWSLYNNMKHPSKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPKEKS-D 150

Query: 123 NMWLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIID 182
             WL TL+ALIGEQF+  DEICG V ++R +Q+++++WTK A+NEAAQ+SIG++WKE +D
Sbjct: 151 KSWLYTLLALIGEQFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLD 210

Query: 183 VNDKISFSFHEDLRR-EKSAKARYS 200
            N+ I F  HED ++ +++AK  Y+
Sbjct: 211 YNNSIGFIIHEDAKKLDRNAKNAYT 234

[Close]
BLAST of Lag0016497 vs. TAIR 10
Match: AT1G29590.1 (Eukaryotic initiation factor 4E protein )

HSP 1 Score: 214.2 bits (544), Expect = 9.9e-56
Identity = 96/180 (53.33%), Postives = 127/180 (70.56%), Query Frame = 0 

Query: 22  QQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQIFKPSKLPANAD 81
           Q+SH  +  WTFWFDN S       WG+SLR +YTF T+EEFW LY+ I  P+K  + +D
Sbjct: 107 QKSHCFQNSWTFWFDNPSSKSNQVIWGSSLRSLYTFATIEEFWSLYNNIHPPTKWVSGSD 166

Query: 82  FHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLETLMALIGEQFEESDEICGVVA 141
            + FK  +EPKWEDP CANGGKWT+   R A L++ WL TL+AL+GEQF++ DEICG V 
Sbjct: 167 LYCFKDKIEPKWEDPICANGGKWTMFFPR-ATLESNWLNTLLALVGEQFDQGDEICGAVL 226

Query: 142 SVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHEDLRR-EKSAKARYSV 201
           + R R D+++LWTK A NE AQ+SIG++WKE++  ND I F  HED +  ++ AK RY+V
Sbjct: 227 NFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDAKRRYTV 285

[Close]
BLAST of Lag0016497 vs. TAIR 10
Match: AT1G29590.2 (Eukaryotic initiation factor 4E protein )

HSP 1 Score: 213.8 bits (543), Expect = 1.3e-55
Identity = 95/180 (52.78%), Postives = 127/180 (70.56%), Query Frame = 0 

Query: 22  QQSHKLERKWTFWFDNQSRPKQGAAWGTSLRKVYTFDTVEEFWCLYDQIFKPSKLPANAD 81
           Q+SH  +  WTFWFDN S       WG+SLR +YTF T+EEFW LY+ I  P+K  + +D
Sbjct: 62  QKSHCFQNSWTFWFDNPSSKSNQVIWGSSLRSLYTFATIEEFWSLYNNIHPPTKWVSGSD 121

Query: 82  FHLFKSGVEPKWEDPECANGGKWTVTSSRKANLDNMWLETLMALIGEQFEESDEICGVVA 141
            + FK  +EPKWEDP CANGGKWT+   R A L++ WL TL+AL+GEQF++ DEICG V 
Sbjct: 122 LYCFKDKIEPKWEDPICANGGKWTMFFPR-ATLESNWLNTLLALVGEQFDQGDEICGAVL 181

Query: 142 SVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHEDLRR-EKSAKARYSV 201
           + R R D+++LWTK A NE AQ+SIG++WKE++  ND I F  HED +  ++ AK RY++
Sbjct: 182 NFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDAKRRYTI 240

The following BLAST results are available for this feature:
BLAST of Lag0016497 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022138462.11.6e-10897.00eukaryotic translation initiation factor [Momordica charantia][more]
XP_008460683.13.0e-10796.06PREDICTED: eukaryotic translation initiation factor [Cucumis melo] >ABY56090.1 e... [more]
XP_038875852.18.8e-10796.52eukaryotic translation initiation factor [Benincasa hispida][more]
XP_004147164.11.5e-10695.57eukaryotic translation initiation factor [Cucumis sativus] >ABY56102.1 eukaryoti... [more]
AJA90794.12.6e-10695.57eukaryotic translation inititation factor (iso)4E [Citrullus lanatus][more]
BLAST of Lag0016497 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q033897.8e-8272.73Eukaryotic translation initiation factor isoform 4E-2 OS=Triticum aestivum OX=45... [more]
P486002.3e-8172.45Eukaryotic translation initiation factor isoform 4E-2 OS=Oryza sativa subsp. jap... [more]
O814827.6e-7767.54Eukaryotic translation initiation factor isoform 4E-2 OS=Zea mays OX=4577 PE=2 S... [more]
O046632.4e-7068.82Eukaryotic translation initiation factor isoform 4E OS=Arabidopsis thaliana OX=3... [more]
O814819.3e-5955.98Eukaryotic translation initiation factor 4E-1 OS=Zea mays OX=4577 PE=2 SV=1[more]
BLAST of Lag0016497 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CB617.8e-10997.00mRNA cap-binding protein OS=Momordica charantia OX=3673 GN=LOC111009629 PE=3 SV=... [more]
B0F8161.5e-10796.06mRNA cap-binding protein OS=Cucumis melo OX=3656 GN=eIF(iso)4E PE=3 SV=1[more]
B0F8327.3e-10795.57mRNA cap-binding protein OS=Cucumis sativus OX=3659 GN=eIF(iso)4E PE=3 SV=1[more]
A0A0D3N9421.2e-10695.57mRNA cap-binding protein OS=Citrullus lanatus OX=3654 GN=eIF4E(iso) PE=3 SV=1[more]
A0A6J1KKA72.0e-10495.00mRNA cap-binding protein OS=Cucurbita maxima OX=3661 GN=LOC111494789 PE=3 SV=1[more]
BLAST of Lag0016497 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G35620.11.7e-7168.82Eukaryotic initiation factor 4E protein [more]
AT5G35620.21.1e-5970.97Eukaryotic initiation factor 4E protein [more]
AT4G18040.12.6e-5650.73eukaryotic translation initiation factor 4E [more]
AT1G29590.19.9e-5653.33Eukaryotic initiation factor 4E protein [more]
AT1G29590.21.3e-5552.78Eukaryotic initiation factor 4E protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 10..30
NoneNo IPR availablePANTHERPTHR11960:SF47BNAA08G29940D PROTEINcoord: 7..192
IPR001040Translation Initiation factor eIF- 4ePFAMPF01652IF4Ecoord: 27..182
e-value: 1.4E-55
score: 187.5
IPR001040Translation Initiation factor eIF- 4ePANTHERPTHR11960EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATEDcoord: 7..192
IPR023398Translation Initiation factor eIF- 4e-likeGENE3D3.30.760.10RNA Cap, Translation Initiation Factor Eif4ecoord: 15..200
e-value: 1.1E-69
score: 235.6
IPR023398Translation Initiation factor eIF- 4e-likeSUPERFAMILY55418eIF4e-likecoord: 21..193
IPR019770Eukaryotic translation initiation factor 4E (eIF-4E), conserved sitePROSITEPS00813IF4Ecoord: 81..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016497.1Lag0016497.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050687 negative regulation of defense response to virus
biological_process GO:0006417 regulation of translation
biological_process GO:0006413 translational initiation
cellular_component GO:0016281 eukaryotic translation initiation factor 4F complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0000340 RNA 7-methylguanosine cap binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0003723 RNA binding