Lag0016476 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0016476
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Beta-amylase
Location	chr12: 38159381 .. 38159941 (+)
RNA-Seq Expression	Lag0016476
Synteny	Lag0016476

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACGAGTTCACTGCACCAGATCGGTACTCTGTTTGCGGCAACGACTGCGAAATGCAACGATCATCCACCTCAATCGAAAGCTCCGGCGAGTGCTGCGCTGGCGAGGACGGTTCCTACGGCGGCGATTTTGCGATCTAGGGCTCCGAATCGAGAAAGAGAGAACGTAACCGTATCATCGTCGTCGGACGGATTCCGGCGGAGTTTGTCTGTGGCGTGCAGAGGATCGGCAGCGGCGGAGGAGGGAGAGAGAGGGAGAGGAGTGCCGGTGTTTGTGATGATGCCGTTGGAGAGCGTGACGATGGAGAATAAGGTGAAGCGGAGGAAGGCGATGGAGATGAGTTTTCAGGCGATGGAATGGGCGGGAGTTGAGGGAGTGATGGTGGACGTGTGGTGGGGATTGGTGGAGAAGGAGAGGCCTGGTGAGTATAACTTTGGAGGTTATGAGGAGCTTTTGGCTATGGCCAGTTCGGCCTCAAAGTTCAGGCCGTCATGTCGTTCCATCAATGCGGAGGCAACGTCGGCGATTCCTGCACGTAACTACTTTCTCCTTCCTTAA

mRNA sequence

ATGGCGACGAGTTCACTGCACCAGATCGGTACTCTGTTTGCGGCAACGACTGCGAAATGCAACGATCATCCACCTCAATCGAAAGCTCCGGCGAGTGCTGCGCTGGCGAGGACGGTTCCTACGGCGGCGATTTTGCGATCTAGGGCTCCGAATCGAGAAAGAGAGAACGTAACCGTATCATCGTCGTCGGACGGATTCCGGCGGAGTTTGTCTGTGGCGTGCAGAGGATCGGCAGCGGCGGAGGAGGGAGAGAGAGGGAGAGGAGTGCCGGTGTTTGTGATGATGCCGTTGGAGAGCGTGACGATGGAGAATAAGGTGAAGCGGAGGAAGGCGATGGAGATGAGTTTTCAGGCGATGGAATGGGCGGGAGTTGAGGGAGTGATGGTGGACGTGTGGTGGGGATTGGTGGAGAAGGAGAGGCCTGGTGAGTATAACTTTGGAGGTTATGAGGAGCTTTTGGCTATGGCCAGTTCGGCCTCAAAGTTCAGGCCGTCATGTCGTTCCATCAATGCGGAGGCAACGTCGGCGATTCCTGCACGTAACTACTTTCTCCTTCCTTAA

Coding sequence (CDS)

ATGGCGACGAGTTCACTGCACCAGATCGGTACTCTGTTTGCGGCAACGACTGCGAAATGCAACGATCATCCACCTCAATCGAAAGCTCCGGCGAGTGCTGCGCTGGCGAGGACGGTTCCTACGGCGGCGATTTTGCGATCTAGGGCTCCGAATCGAGAAAGAGAGAACGTAACCGTATCATCGTCGTCGGACGGATTCCGGCGGAGTTTGTCTGTGGCGTGCAGAGGATCGGCAGCGGCGGAGGAGGGAGAGAGAGGGAGAGGAGTGCCGGTGTTTGTGATGATGCCGTTGGAGAGCGTGACGATGGAGAATAAGGTGAAGCGGAGGAAGGCGATGGAGATGAGTTTTCAGGCGATGGAATGGGCGGGAGTTGAGGGAGTGATGGTGGACGTGTGGTGGGGATTGGTGGAGAAGGAGAGGCCTGGTGAGTATAACTTTGGAGGTTATGAGGAGCTTTTGGCTATGGCCAGTTCGGCCTCAAAGTTCAGGCCGTCATGTCGTTCCATCAATGCGGAGGCAACGTCGGCGATTCCTGCACGTAACTACTTTCTCCTTCCTTAA

Protein sequence

MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVSSSSDGFRRSLSVACRGSAAAEEGERGRGVPVFVMMPLESVTMENKVKRRKAMEMSFQAMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMASSASKFRPSCRSINAEATSAIPARNYFLLP

Homology

[Close]

BLAST of Lag0016476 vs. NCBI nr
Match: XP_023549359.1 (beta-amylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-50
Identity = 114/160 (71.25%), Postives = 128/160 (80.00%), Query Frame = 0


Query: 1   MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVS 60
           MA  S++QIGTLF  T AKCNDH  ++  PAS A+ RTVP   ILRSR  NRERE V + 
Sbjct: 1   MALRSMNQIGTLF--TAAKCNDHSLRAMVPASTAVVRTVPAVGILRSRVQNREREEVMI- 60

Query: 61  SSSDGFRRSLSVACRGSAAAEEGERG---RGVPVFVMMPLESVTMENKVKRRKAMEMSFQ 120
           SS D FRRSL+V+CR S AAEE E+G   RGV VFVMMPL+ VTMENKVK+RKAME+SFQ
Sbjct: 61  SSFDRFRRSLAVSCRASTAAEEAEKGGERRGVAVFVMMPLDCVTMENKVKKRKAMEVSFQ 120

Query: 121 AMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMAS 158
           AM+ AGVEGVMVDVWWGLVEKERPGEYNFGGYEE  AMA+
Sbjct: 121 AMKGAGVEGVMVDVWWGLVEKERPGEYNFGGYEEFFAMAA 157

[Close]

BLAST of Lag0016476 vs. NCBI nr
Match: XP_022959502.1 (beta-amylase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-49
Identity = 114/160 (71.25%), Postives = 127/160 (79.38%), Query Frame = 0


Query: 1   MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVS 60
           MA  S++QIGTLF A  AKCNDH  ++K PAS A  RTV    ILRSR  NRERE V + 
Sbjct: 1   MALRSMNQIGTLFPA--AKCNDHSLRAKVPASTAAVRTVRAVGILRSRVQNREREEVVI- 60

Query: 61  SSSDGFRRSLSVACRGSAAAEEGERG---RGVPVFVMMPLESVTMENKVKRRKAMEMSFQ 120
           SS D FRRSL+V+CR S AAEE E+G   RGV VFVMMPL+ VTMENKVK+RKAME+SFQ
Sbjct: 61  SSFDRFRRSLAVSCRASTAAEEAEKGGERRGVAVFVMMPLDCVTMENKVKKRKAMEVSFQ 120

Query: 121 AMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMAS 158
           AM+ AGVEGVMVDVWWGLVEKERPGEYNFGGYEE  AMA+
Sbjct: 121 AMKGAGVEGVMVDVWWGLVEKERPGEYNFGGYEEFFAMAA 157

[Close]

BLAST of Lag0016476 vs. NCBI nr
Match: XP_023006377.1 (beta-amylase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-49
Identity = 113/160 (70.62%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 1   MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVS 60
           MA  S++QIGTLF A  AKCNDH  ++K PA  A  RTVP   ILRSR  NRERE V + 
Sbjct: 1   MALRSMNQIGTLFPA--AKCNDHSVRAKVPACTAAVRTVPAVGILRSRVQNREREEVMI- 60

Query: 61  SSSDGFRRSLSVACRGSAAAEEGERG---RGVPVFVMMPLESVTMENKVKRRKAMEMSFQ 120
           SS D FRRS +V+CR S AAEE E+G   RGV VFVMMPL+ VTMENKVK+RKAME+SFQ
Sbjct: 61  SSFDRFRRSFAVSCRASTAAEEAEKGGERRGVAVFVMMPLDCVTMENKVKKRKAMEVSFQ 120

Query: 121 AMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMAS 158
           AM+ AGVEGVMVDVWWGLVEKERPGEYNFGGYEE  AMA+
Sbjct: 121 AMKGAGVEGVMVDVWWGLVEKERPGEYNFGGYEEFFAMAA 157

[Close]

BLAST of Lag0016476 vs. NCBI nr
Match: KAG7013452.1 (Beta-amylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 206.8 bits (525), Expect = 1.6e-49
Identity = 114/160 (71.25%), Postives = 127/160 (79.38%), Query Frame = 0


Query: 1   MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVS 60
           MA  S++QIGTLF A  AKCNDH  ++K PAS A  RTV    ILRSR  NRERE V + 
Sbjct: 1   MALRSMNQIGTLFPA--AKCNDHSLRAKVPASTAAVRTVRAVGILRSRVQNREREEVVI- 60

Query: 61  SSSDGFRRSLSVACRGSAAAEEGERG---RGVPVFVMMPLESVTMENKVKRRKAMEMSFQ 120
           SS D FRRSL+V+CR S AAEE E+G   RGV VFVMMPL+ VTMENKVK+RKAME+SFQ
Sbjct: 61  SSFDRFRRSLAVSCRASMAAEEAEKGGERRGVAVFVMMPLDCVTMENKVKKRKAMEVSFQ 120

Query: 121 AMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMAS 158
           AM+ AGVEGVMVDVWWGLVEKERPGEYNFGGYEE  AMA+
Sbjct: 121 AMKGAGVEGVMVDVWWGLVEKERPGEYNFGGYEEFFAMAA 157

[Close]

BLAST of Lag0016476 vs. NCBI nr
Match: KAG6574880.1 (Beta-amylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.8 bits (525), Expect = 1.6e-49
Identity = 114/160 (71.25%), Postives = 127/160 (79.38%), Query Frame = 0


Query: 1   MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVS 60
           MA  S++QIGTLF A  AKCNDH  ++K PAS A  RTV    ILRSR  NRERE V + 
Sbjct: 1   MALRSMNQIGTLFPA--AKCNDHSLRAKVPASTAAVRTVRAVGILRSRVQNREREEVVI- 60

Query: 61  SSSDGFRRSLSVACRGSAAAEEGERG---RGVPVFVMMPLESVTMENKVKRRKAMEMSFQ 120
           SS D FRRSL+V+CR S AAEE E+G   RGV VFVMMPL+ VTMENKVK+RKAME+SFQ
Sbjct: 61  SSFDRFRRSLAVSCRASMAAEEAEKGGERRGVAVFVMMPLDCVTMENKVKKRKAMEVSFQ 120

Query: 121 AMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMAS 158
           AM+ AGVEGVMVDVWWGLVEKERPGEYNFGGYEE  AMA+
Sbjct: 121 AMKGAGVEGVMVDVWWGLVEKERPGEYNFGGYEEFFAMAA 157

[Close]

BLAST of Lag0016476 vs. ExPASy Swiss-Prot
Match: Q9LIR6 (Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-25
Identity = 78/174 (44.83%), Postives = 96/174 (55.17%), Query Frame = 0


Query: 1   MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVS 60
           MA +  HQ+G L A T  K  +    S    S   AR +   A+ R+   +    +  +S
Sbjct: 1   MALNLSHQLGVL-AGTPIKSGEMTDSSLLSISPPSARMMTPKAMNRNYKAHGTDPSPPMS 60

Query: 61  SSSDGFRRSLSVACRGSAAA------------EEG------ERGRGVPVFVMMPLESVTM 120
                 R  LSVAC+  A               EG      E G GVPVFVMMPL+SVTM
Sbjct: 61  PILGATRADLSVACKAFAVENGIGTIEEQRTYREGGIGGKKEGGGGVPVFVMMPLDSVTM 120

Query: 121 ENKVKRRKAMEMSFQAMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMA 157
            N V RRKAM+ S QA++ AGVEG+M+DVWWGLVEKE PG YN+GGY ELL +A
Sbjct: 121 GNTVNRRKAMKASLQALKSAGVEGIMIDVWWGLVEKESPGTYNWGGYNELLELA 173

[Close]

BLAST of Lag0016476 vs. ExPASy Swiss-Prot
Match: Q9AV88 (Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.3e-18
Identity = 62/135 (45.93%), Postives = 77/135 (57.04%), Query Frame = 0


Query: 32  SAALARTVPTAAILRSRAPNRERENVTVSSSSDGFRRSLSVAC---------RGSAAAEE 91
           SAA A    TA   R RA +      + SS++   R     AC         R  AAA  
Sbjct: 8   SAAAAACFATAGDAR-RAASVVAMPSSSSSATTSLRMKRQAACEPVACRAVARHVAAAAA 67

Query: 92  GERGRGVPVFVMMPLESVT-MENKVKRRKAMEMSFQAMEWAGVEGVMVDVWWGLVEKERP 151
             R  GVPVFVMMPL++V+   + + RRKA+  S  A++ AGVEG+MVDVWWG+VE E P
Sbjct: 68  SSRRNGVPVFVMMPLDTVSKCGSALNRRKAVAASLAALKSAGVEGIMVDVWWGIVESEGP 127

Query: 152 GEYNFGGYEELLAMA 157
           G YNF GY EL+ MA
Sbjct: 128 GRYNFDGYVELMEMA 141

[Close]

BLAST of Lag0016476 vs. ExPASy Swiss-Prot
Match: Q10RZ1 (Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-17
Identity = 67/167 (40.12%), Postives = 92/167 (55.09%), Query Frame = 0


Query: 1   MATSSLHQIGTLFAATTAKCNDHPPQSKAPASAALARTVPTAAILRSRAPNRERENVTVS 60
           M+ +  HQ G   AA  A     P   +    AA A TV   A+  + AP+ + +  TV 
Sbjct: 2   MSLNLAHQTGA--AAAVA-----PAAPRTAVVAAAAGTVSAPAVAPAAAPSLQLQTQTVD 61

Query: 61  SSSDGFRRSLSVACRG--SAAAEE------GERGR--GVPVFVMMPLESVTME-NKVKRR 120
            ++      L +A +    +  EE      GE  R  GVPV+VMMPL++V  + N + RR
Sbjct: 62  PAAPAQGPDLPMAFQALVESLPEEQHPDVGGEERRKVGVPVYVMMPLDTVRKDGNGLNRR 121

Query: 121 KAMEMSFQAMEWAGVEGVMVDVWWGLVEKERPGEYNFGGYEELLAMA 157
           KA+E S +A++ AG EG+MVDVWWG+ E E PG YNF GY EL+ MA
Sbjct: 122 KAVEASLKALKSAGAEGIMVDVWWGIAECEGPGRYNFTGYMELMEMA 161

[Close]

BLAST of Lag0016476 vs. ExPASy Swiss-Prot
Match: O23553 (Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3)

HSP 1 Score: 86.3 bits (212), Expect = 4.1e-16
Identity = 39/67 (58.21%), Postives = 50/67 (74.63%), Query Frame = 0


Query: 89  VPVFVMMPLESVTMENKVKRRKAMEMSFQAMEWAGVEGVMVDVWWGLVEKERPGEYNFGG 148
           VPVFVM+PL++VTM   + + +AM  S  A++ AGVEGVMVD WWGLVEK+ P  YN+ G
Sbjct: 86  VPVFVMLPLDTVTMSGHLNKPRAMNASLMALKGAGVEGVMVDAWWGLVEKDGPMNYNWEG 145

Query: 149 YEELLAM 156
           Y EL+ M
Sbjct: 146 YAELIQM 152

[Close]

BLAST of Lag0016476 vs. ExPASy Swiss-Prot
Match: Q9FM68 (Inactive beta-amylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM4 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.0e-11
Identity = 35/79 (44.30%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 85  RGRGVPVFVMMPLESVTME----NKVKRRKAMEMSFQAMEWAGVEGVMVDVWWGLVEKER 144
           R + VPVFVMMP+++  ++     K+KR KA+ +S +A++ AGV G+ V+VWWG+VE+  
Sbjct: 88  RHKRVPVFVMMPIDTFGIDASGCPKIKRLKALTVSLKALKLAGVHGIAVEVWWGIVERFS 147

Query: 145 PGEYNFGGYEELLAMASSA 160
           P E+ +  YEEL  + S A
Sbjct: 148 PLEFKWSLYEELFRLISEA 166

The following BLAST results are available for this feature:

BLAST of Lag0016476 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023549359.1	1.9e-50	71.25	beta-amylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022959502.1	1.2e-49	71.25	beta-amylase 1, chloroplastic-like [Cucurbita moschata]	[more]
XP_023006377.1	1.2e-49	70.63	beta-amylase 1, chloroplastic-like [Cucurbita maxima]	[more]
KAG7013452.1	1.6e-49	71.25	Beta-amylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
KAG6574880.1	1.6e-49	71.25	Beta-amylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0016476 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIR6	2.8e-25	44.83	Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1	[more]
Q9AV88	3.3e-18	45.93	Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 ...	[more]
Q10RZ1	1.6e-17	40.12	Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 ...	[more]
O23553	4.1e-16	58.21	Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3	[more]
Q9FM68	1.0e-11	44.30	Inactive beta-amylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM4 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 82..160 e-value: 7.9E-25 score: 89.2
None	No IPR available	PANTHER	PTHR31352:SF38	BETA-AMYLASE 1, CHLOROPLASTIC	coord: 17..159
IPR001554	Glycoside hydrolase, family 14	PFAM	PF01373	Glyco_hydro_14	coord: 92..157 e-value: 3.4E-14 score: 52.6
IPR001554	Glycoside hydrolase, family 14	PANTHER	PTHR31352	FAMILY NOT NAMED	coord: 17..159
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 88..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0016476.1	Lag0016476.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000272	polysaccharide catabolic process
molecular_function	GO:0102229	amylopectin maltohydrolase activity
molecular_function	GO:0016161	beta-amylase activity