Lag0016385 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0016385 vs. NCBI nr
Match: XP_038875356.1 (myb-like protein X [Benincasa hispida] >XP_038875357.1 myb-like protein X [Benincasa hispida])

HSP 1 Score: 776.5 bits (2004), Expect = 1.4e-220
Identity = 454/538 (84.39%), Postives = 474/538 (88.10%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKAHALEESRPLGKSGSQNGEEVVRAKHNSVQEEK 120
           EKKDKKKDRDKK      KDR+KEKEKA A EESR  GK GSQNGEE VR KHNSVQEEK
Sbjct: 61  EKKDKKKDRDKK------KDRNKEKEKARAAEESRNSGKIGSQNGEEAVRVKHNSVQEEK 120

Query: 121 HAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMAT 180
           HAGQFDSYVGNKISQNAFLSKE KNSK+V E+GRRIED GTAKV   A+AQP+RDDGM T
Sbjct: 121 HAGQFDSYVGNKISQNAFLSKEAKNSKMVLEVGRRIEDGGTAKVEKFAVAQPRRDDGMVT 180

Query: 181 QVARSSETLFEGKEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTTTATA 240
           Q+ RSSETLFE KEQ+KNKKIDERKYNGQGIRHEERFSGNSAVPSS              
Sbjct: 181 QMGRSSETLFEAKEQSKNKKIDERKYNGQGIRHEERFSGNSAVPSS------------AT 240

Query: 241 TATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDKES 300
           TATAT++SGV GMFKQ+EK  ERRKE NDK RQKEG+EKR  KHKDKDKEKKGRSNDKES
Sbjct: 241 TATATVESGVPGMFKQMEKSAERRKEANDKTRQKEGEEKR-SKHKDKDKEKKGRSNDKES 300

Query: 301 DKEK---KKEKKAKEKEKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSNISAVV 360
           DKEK   KKEKK KEKE VEEKNAMVDKTKE+NKDNLL GRHSTNTNTSQLPDSNI A V
Sbjct: 301 DKEKKKEKKEKKVKEKEMVEEKNAMVDKTKEINKDNLL-GRHSTNTNTSQLPDSNIGAAV 360

Query: 361 EENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDRQAVAN 420
           EENLIKKRKDFEPNGVLHAIDNRS KLL+  SHPLKENGRILEPC TS LPPSDRQAV+N
Sbjct: 361 EENLIKKRKDFEPNGVLHAIDNRSGKLLKPNSHPLKENGRILEPCSTSPLPPSDRQAVSN 420

Query: 421 DFLLGSKERKVNGIIEAHHPPASSKHTPGGQDDHPQPSAIHKKSPHPDSKYLSLVYSVPK 480
           D +L SKERK+NGIIEAHHPPASSKH  GGQDDH QP+ IHKKSPHPDSK+LS VYSVPK
Sbjct: 421 DLILVSKERKINGIIEAHHPPASSKHKSGGQDDHLQPTPIHKKSPHPDSKHLSQVYSVPK 480

Query: 481 MEELSDSDNKDWLFSSNTSQVMKPKLEASEAEEMPQVWAEAMQLESADVYALPYVIPY 533
           MEELSDSDN+DWLFSSNTS VMKPKLEASEAEEMPQVWAEAMQ+ESADVYALPYVIPY
Sbjct: 481 MEELSDSDNQDWLFSSNTSLVMKPKLEASEAEEMPQVWAEAMQIESADVYALPYVIPY 518

BLAST of Lag0016385 vs. NCBI nr
Match: XP_004139013.2 (glutamic acid-rich protein [Cucumis sativus] >KGN61495.1 hypothetical protein Csa_006562 [Cucumis sativus])

HSP 1 Score: 761.9 bits (1966), Expect = 3.6e-216
Identity = 448/537 (83.43%), Postives = 482/537 (89.76%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKAHALEESRPLGKSGSQNGEEVVRAKHNSVQEEK 120
           EKKDKKKDRDKK      KDR+KEKEKAHA EES+  GK GSQNGEEVVR KHNSVQEEK
Sbjct: 61  EKKDKKKDRDKK------KDRNKEKEKAHASEESKHSGKIGSQNGEEVVRVKHNSVQEEK 120

Query: 121 HAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMAT 180
           HAGQFDSYVGNKISQNAF SKETK++K+V E+GRRIEDSGTAKV   A+AQP+RDDGM T
Sbjct: 121 HAGQFDSYVGNKISQNAFHSKETKHTKMVLEVGRRIEDSGTAKVEKFAVAQPRRDDGMVT 180

Query: 181 QVARSSETLFEGKEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSS--TATTTATTTTTA 240
           QV R SETL E K+Q+KNKKIDERKYNGQG+RHEERFSG+SAVPSS  T+T TAT T TA
Sbjct: 181 QVGRISETLLETKDQSKNKKIDERKYNGQGLRHEERFSGSSAVPSSAPTSTATATATATA 240

Query: 241 TATATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDK 300
           TATATAT++ GV GMFKQLEK  ERRKE+NDK RQKEG+E+R  KHKDKDKEKKGRSNDK
Sbjct: 241 TATATATVEPGVPGMFKQLEKSAERRKESNDKTRQKEGEERR-SKHKDKDKEKKGRSNDK 300

Query: 301 ESDKEKKKEKKAKEKEKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSNISAVVE 360
           + +KEKKKEKKA  KEKVEEKNAMVDKTKE+NKDNLL GRHSTNTNTSQLPDSNI A VE
Sbjct: 301 QGNKEKKKEKKA--KEKVEEKNAMVDKTKEINKDNLL-GRHSTNTNTSQLPDSNIVAAVE 360

Query: 361 ENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDRQAVAND 420
           ENLIKKRKDFEPNGVLHAIDNRSSKLLR TSH LKENGRILEPCL S+LPPS+RQAV+ND
Sbjct: 361 ENLIKKRKDFEPNGVLHAIDNRSSKLLRPTSHSLKENGRILEPCLRSTLPPSERQAVSND 420

Query: 421 FLLGSKERKVNGIIEAHHPPASSKHTPGGQDDHPQPSAIHKKSPHPDSKYLSLVYSVPKM 480
            +L SKERK+NGIIEAHHPPASSKH   GQ DHPQP A+HKKSPHPDSK+LS VYSVPKM
Sbjct: 421 LILVSKERKINGIIEAHHPPASSKHR-SGQVDHPQPPAMHKKSPHPDSKHLSQVYSVPKM 480

Query: 481 EELSDSDNKDWLFSSNTSQVMKPKLEASEAEEMPQVWAEAMQLESADVYALPYVIPY 533
           EELSDSD++DWLFS N+S  MKPKLE SEA+EMP VWA+AMQ+ESADVYALPYVIPY
Sbjct: 481 EELSDSDSQDWLFSGNSSLAMKPKLEVSEAKEMPNVWADAMQIESADVYALPYVIPY 526

BLAST of Lag0016385 vs. NCBI nr
Match: XP_022138508.1 (DNA ligase 1 [Momordica charantia] >XP_022138509.1 DNA ligase 1 [Momordica charantia])

HSP 1 Score: 744.6 bits (1921), Expect = 6.0e-211
Identity = 438/544 (80.51%), Postives = 474/544 (87.13%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKD+SDEKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDRSDEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKAHALEESRPLGKSGSQNGEEVVRAKHNSVQEEK 120
           EKKDKKKDRDKKKDRDK KD++KEKEKAHA EESRPLG++GSQNGEEVVR KHN+VQEEK
Sbjct: 61  EKKDKKKDRDKKKDRDK-KDKEKEKEKAHASEESRPLGETGSQNGEEVVRVKHNNVQEEK 120

Query: 121 HAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMAT 180
           + GQFDSYVGNKISQNAFLSKETKN+ L PE+GRRIED GT K+   A+A+PKRDDGMAT
Sbjct: 121 NGGQFDSYVGNKISQNAFLSKETKNTTLFPEVGRRIEDIGTTKLEKFAVARPKRDDGMAT 180

Query: 181 QVARSSETLFEGKEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTTTATA 240
            VARSSET  EGKEQNKN+KIDER YNGQGIRHEERFSGNSAVPSS              
Sbjct: 181 VVARSSETFTEGKEQNKNRKIDERTYNGQGIRHEERFSGNSAVPSS-------------- 240

Query: 241 TATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDKES 300
           TATAT++SGVAGMFK L+K  ERRKETNDKIR KE + K+G KHKDKDK+KKGRSN KE 
Sbjct: 241 TATATVESGVAGMFKSLDKVAERRKETNDKIRPKESEGKQGSKHKDKDKDKKGRSNHKEK 300

Query: 301 DKEKK-------KEK-KAKEKEKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSN 360
            KEKK       KEK K KEKEKVEEKNAMVDKTKE+NKDNLL GR+STNTNTSQLPDSN
Sbjct: 301 KKEKKAKEKEKEKEKQKEKEKEKVEEKNAMVDKTKEINKDNLL-GRYSTNTNTSQLPDSN 360

Query: 361 ISAVVEENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDR 420
           I A VEE LIKKRKDFEPNG+LHAIDNRSSKLLR T+HPLKENGRIL+PC+TS LPPS+ 
Sbjct: 361 IGAAVEEKLIKKRKDFEPNGILHAIDNRSSKLLRPTAHPLKENGRILKPCVTSILPPSES 420

Query: 421 QAVANDFLLGSKERKVNGIIEAHHP-PASSKHTPGGQDDHPQPSAIHKKSPHPDSKYLSL 480
           QAVAND + GSKERK+NGIIEAHH  PASSKH   GQDD PQP+ +HKKSPHPDSKYLS 
Sbjct: 421 QAVANDLIPGSKERKINGIIEAHHHLPASSKHVSSGQDDQPQPTVVHKKSPHPDSKYLSQ 480

Query: 481 VYSVPKMEELSDSDNKDWLFSSNTSQVMKPKLEASEAEEMPQVWAEAMQLESADVYALPY 533
           VYSVPKMEELSDSD+++WLFSSNTSQ MKPKLE +E EE  QVWA+AMQ+ESADVYALPY
Sbjct: 481 VYSVPKMEELSDSDSQEWLFSSNTSQAMKPKLEDTEGEETRQVWADAMQIESADVYALPY 528

BLAST of Lag0016385 vs. NCBI nr
Match: XP_023513944.1 (DNA ligase 1-like [Cucurbita pepo subsp. pepo] >XP_023513947.1 DNA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 715.7 bits (1846), Expect = 3.0e-202
Identity = 436/564 (77.30%), Postives = 474/564 (84.04%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEK--KEKEKREGKEKRDKDKSDEK 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEK  KEKEK+EGKEKRDKDKS+EK
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKEKKEGKEKRDKDKSEEK 60

Query: 61  RREKKDKKKDRDKKKDRDKEKDRDKEKEKA-HALEESRPLGKSGSQNGEEVVRAKHNSVQ 120
           RREKKDKKKDRDKKK+RDKEK+++KEKEKA HALE+SRP GK  SQNGEEVVR KHNS+Q
Sbjct: 61  RREKKDKKKDRDKKKERDKEKEKEKEKEKAPHALEDSRPSGKIKSQNGEEVVRIKHNSIQ 120

Query: 121 EEKHAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDG 180
           EEKH+G+ DSYVGNKISQN  L KETKNSKLVPE+GRRIEDSGTAKV   A+AQPKRDDG
Sbjct: 121 EEKHSGKLDSYVGNKISQNTVLPKETKNSKLVPEVGRRIEDSGTAKVEKLAVAQPKRDDG 180

Query: 181 MATQVARSSETLFEG-KEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTT 240
           +ATQVARSSE LFEG KE  KNK ID+ KYNGQGIRH+ERFSG+S VPS TATT      
Sbjct: 181 VATQVARSSEILFEGNKEHIKNKTIDQGKYNGQGIRHDERFSGDSTVPSFTATT------ 240

Query: 241 TATATATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSN 300
             T TA ATI+S         EK  ERRKETNDKIR KEG+EKRG KHKDKDKEKKGRSN
Sbjct: 241 --TTTANATIESA--------EKIAERRKETNDKIRPKEGEEKRGSKHKDKDKEKKGRSN 300

Query: 301 DKESDKEKKKEKKAKEK----EKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSN 360
           DK +DKEKKKEKKAKEK    ++ EEKNA+VDKTKE+ KDN L+GRHSTNT+TSQLPDSN
Sbjct: 301 DKATDKEKKKEKKAKEKKAKQKEEEEKNAVVDKTKEITKDN-LIGRHSTNTDTSQLPDSN 360

Query: 361 ISAVVEENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDR 420
           I A VEENLIKKRK FEPNGVLHAIDNRSSKLLR  SHPLKENGRILEPC+ S LPPS+R
Sbjct: 361 IGAAVEENLIKKRKAFEPNGVLHAIDNRSSKLLRPASHPLKENGRILEPCMASPLPPSER 420

Query: 421 QAV-ANDFLLGSKERKVNGIIEAHHPPASSKHTPGGQDD--------------------H 480
           Q V AND +L SKERK+NGIIEAHHPPAS KH  GGQDD                     
Sbjct: 421 QTVAANDVILVSKERKINGIIEAHHPPASLKHKTGGQDDQPQPQPQPQLQLQLQLQPQLQ 480

Query: 481 PQPSAIHKKSPHPDSKYLSLVYSVPKMEELSDSDNKDWLFSSNTSQVMKPKLEASEAEEM 533
           PQP+AIHKKSPHPDSK+LS+VYSVPKMEELSDSDN+DWLF+SNTSQ MKP+LEASEA+EM
Sbjct: 481 PQPTAIHKKSPHPDSKFLSVVYSVPKMEELSDSDNQDWLFTSNTSQAMKPELEASEAKEM 540

BLAST of Lag0016385 vs. NCBI nr
Match: KAG6593755.1 (hypothetical protein SDJN03_13231, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 711.1 bits (1834), Expect = 7.3e-201
Identity = 431/569 (75.75%), Postives = 472/569 (82.95%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEK+EGKEKRDKDKS+EKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKKEGKEKRDKDKSEEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKA-HALEESRPLGKSGSQNGEEVVRAKHNSVQEE 120
           EKKDKKKDRDKKK+RDKEK+++KEKEKA HALE+S+P GK  SQNGEEVVR KHNS+QEE
Sbjct: 61  EKKDKKKDRDKKKERDKEKEKEKEKEKAPHALEDSKPSGKIKSQNGEEVVRIKHNSIQEE 120

Query: 121 KHAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMA 180
           KH+G+ DSYVGNKISQN  L KETKNSKLVPE+GRRIEDSGTAKV   A+AQPKRDDG+A
Sbjct: 121 KHSGKLDSYVGNKISQNTVLPKETKNSKLVPEVGRRIEDSGTAKVEKLAVAQPKRDDGVA 180

Query: 181 TQVARSSETLFEG-KEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTTTA 240
           TQVARSSE LFEG KE  KNK ID+ KYNGQGIRH+ERFSG+S VPS TATT        
Sbjct: 181 TQVARSSEILFEGNKEHIKNKTIDQGKYNGQGIRHDERFSGDSTVPSFTATT-------- 240

Query: 241 TATATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDK 300
             TA AT++S         EK  ERRKETNDKIR KEG+EKRG KHKDKDKEKKGRSNDK
Sbjct: 241 ITTANATVESA--------EKIAERRKETNDKIRPKEGEEKRGSKHKDKDKEKKGRSNDK 300

Query: 301 ESDKEKKKEKKAKEK-------EKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDS 360
            +DKEKKKEKKAKEK       ++ EEKNA+VDKTKE+ KDN L+GRHSTNT+TSQLPD 
Sbjct: 301 ATDKEKKKEKKAKEKKEKKAKQKEEEEKNAVVDKTKEITKDN-LIGRHSTNTDTSQLPDG 360

Query: 361 NISAVVEENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSD 420
           NI A VEENL+KKRK FEPNGVLHAIDNRSSKLLR  SHPLKENGRILEPCL S LPPS+
Sbjct: 361 NIGAAVEENLVKKRKAFEPNGVLHAIDNRSSKLLRPASHPLKENGRILEPCLASPLPPSE 420

Query: 421 RQAV-ANDFLLGSKERKVNGIIEAHHPPASSKHTPGGQDDH------------------- 480
           RQ V AND +L SKERK+NGIIEAHH PAS KH  GGQDDH                   
Sbjct: 421 RQTVAANDVILVSKERKINGIIEAHHRPASLKHKTGGQDDHPQPQPQPQPQPQPQLQLQL 480

Query: 481 -----PQPSAIHKKSPHPDSKYLSLVYSVPKMEELSDSDNKDWLFSSNTSQVMKPKLEAS 533
                PQP+AIHKKSPHPDSK+LS+VYSVPKMEELS+SDN+DWLF+SNTSQ MKP+LEAS
Sbjct: 481 QPQPQPQPTAIHKKSPHPDSKFLSVVYSVPKMEELSESDNQDWLFTSNTSQAMKPELEAS 540

BLAST of Lag0016385 vs. ExPASy TrEMBL
Match: A0A0A0LHZ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G147910 PE=4 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.8e-216
Identity = 448/537 (83.43%), Postives = 482/537 (89.76%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKAHALEESRPLGKSGSQNGEEVVRAKHNSVQEEK 120
           EKKDKKKDRDKK      KDR+KEKEKAHA EES+  GK GSQNGEEVVR KHNSVQEEK
Sbjct: 61  EKKDKKKDRDKK------KDRNKEKEKAHASEESKHSGKIGSQNGEEVVRVKHNSVQEEK 120

Query: 121 HAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMAT 180
           HAGQFDSYVGNKISQNAF SKETK++K+V E+GRRIEDSGTAKV   A+AQP+RDDGM T
Sbjct: 121 HAGQFDSYVGNKISQNAFHSKETKHTKMVLEVGRRIEDSGTAKVEKFAVAQPRRDDGMVT 180

Query: 181 QVARSSETLFEGKEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSS--TATTTATTTTTA 240
           QV R SETL E K+Q+KNKKIDERKYNGQG+RHEERFSG+SAVPSS  T+T TAT T TA
Sbjct: 181 QVGRISETLLETKDQSKNKKIDERKYNGQGLRHEERFSGSSAVPSSAPTSTATATATATA 240

Query: 241 TATATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDK 300
           TATATAT++ GV GMFKQLEK  ERRKE+NDK RQKEG+E+R  KHKDKDKEKKGRSNDK
Sbjct: 241 TATATATVEPGVPGMFKQLEKSAERRKESNDKTRQKEGEERR-SKHKDKDKEKKGRSNDK 300

Query: 301 ESDKEKKKEKKAKEKEKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSNISAVVE 360
           + +KEKKKEKKA  KEKVEEKNAMVDKTKE+NKDNLL GRHSTNTNTSQLPDSNI A VE
Sbjct: 301 QGNKEKKKEKKA--KEKVEEKNAMVDKTKEINKDNLL-GRHSTNTNTSQLPDSNIVAAVE 360

Query: 361 ENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDRQAVAND 420
           ENLIKKRKDFEPNGVLHAIDNRSSKLLR TSH LKENGRILEPCL S+LPPS+RQAV+ND
Sbjct: 361 ENLIKKRKDFEPNGVLHAIDNRSSKLLRPTSHSLKENGRILEPCLRSTLPPSERQAVSND 420

Query: 421 FLLGSKERKVNGIIEAHHPPASSKHTPGGQDDHPQPSAIHKKSPHPDSKYLSLVYSVPKM 480
            +L SKERK+NGIIEAHHPPASSKH   GQ DHPQP A+HKKSPHPDSK+LS VYSVPKM
Sbjct: 421 LILVSKERKINGIIEAHHPPASSKHR-SGQVDHPQPPAMHKKSPHPDSKHLSQVYSVPKM 480

Query: 481 EELSDSDNKDWLFSSNTSQVMKPKLEASEAEEMPQVWAEAMQLESADVYALPYVIPY 533
           EELSDSD++DWLFS N+S  MKPKLE SEA+EMP VWA+AMQ+ESADVYALPYVIPY
Sbjct: 481 EELSDSDSQDWLFSGNSSLAMKPKLEVSEAKEMPNVWADAMQIESADVYALPYVIPY 526

BLAST of Lag0016385 vs. ExPASy TrEMBL
Match: A0A6J1CD63 (DNA ligase 1 OS=Momordica charantia OX=3673 GN=LOC111009661 PE=4 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.9e-211
Identity = 438/544 (80.51%), Postives = 474/544 (87.13%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKD+SDEKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDRSDEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKAHALEESRPLGKSGSQNGEEVVRAKHNSVQEEK 120
           EKKDKKKDRDKKKDRDK KD++KEKEKAHA EESRPLG++GSQNGEEVVR KHN+VQEEK
Sbjct: 61  EKKDKKKDRDKKKDRDK-KDKEKEKEKAHASEESRPLGETGSQNGEEVVRVKHNNVQEEK 120

Query: 121 HAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMAT 180
           + GQFDSYVGNKISQNAFLSKETKN+ L PE+GRRIED GT K+   A+A+PKRDDGMAT
Sbjct: 121 NGGQFDSYVGNKISQNAFLSKETKNTTLFPEVGRRIEDIGTTKLEKFAVARPKRDDGMAT 180

Query: 181 QVARSSETLFEGKEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTTTATA 240
            VARSSET  EGKEQNKN+KIDER YNGQGIRHEERFSGNSAVPSS              
Sbjct: 181 VVARSSETFTEGKEQNKNRKIDERTYNGQGIRHEERFSGNSAVPSS-------------- 240

Query: 241 TATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDKES 300
           TATAT++SGVAGMFK L+K  ERRKETNDKIR KE + K+G KHKDKDK+KKGRSN KE 
Sbjct: 241 TATATVESGVAGMFKSLDKVAERRKETNDKIRPKESEGKQGSKHKDKDKDKKGRSNHKEK 300

Query: 301 DKEKK-------KEK-KAKEKEKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSN 360
            KEKK       KEK K KEKEKVEEKNAMVDKTKE+NKDNLL GR+STNTNTSQLPDSN
Sbjct: 301 KKEKKAKEKEKEKEKQKEKEKEKVEEKNAMVDKTKEINKDNLL-GRYSTNTNTSQLPDSN 360

Query: 361 ISAVVEENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDR 420
           I A VEE LIKKRKDFEPNG+LHAIDNRSSKLLR T+HPLKENGRIL+PC+TS LPPS+ 
Sbjct: 361 IGAAVEEKLIKKRKDFEPNGILHAIDNRSSKLLRPTAHPLKENGRILKPCVTSILPPSES 420

Query: 421 QAVANDFLLGSKERKVNGIIEAHHP-PASSKHTPGGQDDHPQPSAIHKKSPHPDSKYLSL 480
           QAVAND + GSKERK+NGIIEAHH  PASSKH   GQDD PQP+ +HKKSPHPDSKYLS 
Sbjct: 421 QAVANDLIPGSKERKINGIIEAHHHLPASSKHVSSGQDDQPQPTVVHKKSPHPDSKYLSQ 480

Query: 481 VYSVPKMEELSDSDNKDWLFSSNTSQVMKPKLEASEAEEMPQVWAEAMQLESADVYALPY 533
           VYSVPKMEELSDSD+++WLFSSNTSQ MKPKLE +E EE  QVWA+AMQ+ESADVYALPY
Sbjct: 481 VYSVPKMEELSDSDSQEWLFSSNTSQAMKPKLEDTEGEETRQVWADAMQIESADVYALPY 528

BLAST of Lag0016385 vs. ExPASy TrEMBL
Match: A0A6J1KEE4 (myb-like protein X isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111494242 PE=4 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.4e-197
Identity = 428/556 (76.98%), Postives = 464/556 (83.45%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEK+EGKEKRDKDKS+EKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKKEGKEKRDKDKSEEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKA-HALEESRPLGKSGSQNGEEVVRAKHNSVQEE 120
           EKKDK KDRDKKK+RDKE    KEKEKA HALE+SR  GK  SQNGEEVVR KHNS+QEE
Sbjct: 61  EKKDKNKDRDKKKERDKE----KEKEKAPHALEDSRSSGKIKSQNGEEVVRIKHNSIQEE 120

Query: 121 KHAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMA 180
           KH+G+ DSYVGNKISQN  L KETKNSKLVPE+GRRIEDSGTAKV   A+AQPKRDDG+A
Sbjct: 121 KHSGKLDSYVGNKISQNTVLPKETKNSKLVPEVGRRIEDSGTAKVEKLAVAQPKRDDGVA 180

Query: 181 TQVARSSETLFEG-KEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTTTA 240
           TQVARSSE LFEG KE  KNK ID+ KYNGQGIRH+ERFSG+S VPS TATT        
Sbjct: 181 TQVARSSEILFEGNKEHIKNKTIDQGKYNGQGIRHDERFSGDSTVPSFTATT-------- 240

Query: 241 TATATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDK 300
           T TA AT++S         EK  E+RKETNDKIR KEG+ KRG KHKDKDKEKKGRSNDK
Sbjct: 241 TTTANATVESA--------EKIAEKRKETNDKIRPKEGEAKRGSKHKDKDKEKKGRSNDK 300

Query: 301 ESDKEKKKEKKAKEK----EKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSNIS 360
            +DKEKKKEKKAKEK    ++ EEKNA+VDKTKE+ KDN L+GRHST+TNTSQLPDSNI 
Sbjct: 301 ATDKEKKKEKKAKEKKAKQKEEEEKNAVVDKTKEITKDN-LIGRHSTDTNTSQLPDSNIG 360

Query: 361 AVVEENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDRQA 420
           A VEENLIKKRK FEPNGVLHAIDNRSSKLLR  SHPLKENGRILEPCL S LPPS+RQ 
Sbjct: 361 AAVEENLIKKRKAFEPNGVLHAIDNRSSKLLRPASHPLKENGRILEPCLASPLPPSERQT 420

Query: 421 V-ANDFLLGSKERKVNGIIEAHHPPASSKHTPGGQDDH--------------PQPSAIHK 480
           V AND +L SKERK+NGIIEAHHPPAS KH    QDDH              PQP+AIHK
Sbjct: 421 VEANDVILFSKERKINGIIEAHHPPASLKHKTSEQDDHPQSQPQPQPQLQLQPQPTAIHK 480

Query: 481 KSPHPDSKYLSLVYSVPKMEELSDSDNKDWLFSSNTSQVMKPKLEASEAEEMPQVWAEAM 533
           KSPHPDSK+LS+VYSVPKMEELSDSDN+DWLF+SN SQ MKP+LEASEA+EM QVWAEAM
Sbjct: 481 KSPHPDSKFLSVVYSVPKMEELSDSDNQDWLFTSNNSQAMKPELEASEAKEMLQVWAEAM 535

BLAST of Lag0016385 vs. ExPASy TrEMBL
Match: A0A6J1HKL8 (DNA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111464404 PE=4 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 3.1e-197
Identity = 424/568 (74.65%), Postives = 463/568 (81.51%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEK+EGKEKRDKDKS+EKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKKEGKEKRDKDKSEEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKAHALEESRPLGKSGSQNGEEVVRAKHNSVQEEK 120
           EKKDKKKDRDKKK+RDKEK++       HALE+SRP GK  SQNGEEVVR KHNS+QEEK
Sbjct: 61  EKKDKKKDRDKKKERDKEKEK-----APHALEDSRPSGKIKSQNGEEVVRIKHNSIQEEK 120

Query: 121 HAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMAT 180
           H+G+ DSYVGNKISQN  L KETKNSKLVPE+GRRIEDSGTAKV   A+AQPKRDDG+AT
Sbjct: 121 HSGKLDSYVGNKISQNTVLPKETKNSKLVPEVGRRIEDSGTAKVEKLAVAQPKRDDGVAT 180

Query: 181 QVARSSETLFEG-KEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTTTAT 240
           QVARSSE L EG KE  KNK ID+ KYNGQGIRH+ERFSG+S VPS TATT         
Sbjct: 181 QVARSSEILLEGNKEHIKNKTIDQGKYNGQGIRHDERFSGDSTVPSFTATT--------I 240

Query: 241 ATATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDKE 300
            TA AT++S         EK  ERRKETNDKIR KEG+EKRG KHKDKDKEKKGRSNDK 
Sbjct: 241 TTANATVESA--------EKIAERRKETNDKIRPKEGEEKRGSKHKDKDKEKKGRSNDKA 300

Query: 301 SDKEKKKEKKAKEK-------EKVEEKNAMVDKTKEVNKDNLLVGRHSTNTNTSQLPDSN 360
           +DKEKKKEKK+KEK       ++ EEKNA+VDKTKE+ KDN L+GRHSTNT+TSQLPD N
Sbjct: 301 TDKEKKKEKKSKEKKEKKAKQKEEEEKNAVVDKTKEITKDN-LIGRHSTNTDTSQLPDGN 360

Query: 361 ISAVVEENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCLTSSLPPSDR 420
           I A VEENL+KKRK FEPNGVLHAIDNRSSKLLR  SHPLKENGRILEPCL S LPPS+R
Sbjct: 361 IGAAVEENLVKKRKAFEPNGVLHAIDNRSSKLLRPASHPLKENGRILEPCLASPLPPSER 420

Query: 421 QAV-ANDFLLGSKERKVNGIIEAHHPPASSKHTPGGQDDH-------------------- 480
           Q V AND +L SKERK+NGIIEAHHPPAS KH  GGQDDH                    
Sbjct: 421 QTVAANDVILVSKERKINGIIEAHHPPASLKHKTGGQDDHPQLQLQLQPQLQLQLQPQLQ 480

Query: 481 ----PQPSAIHKKSPHPDSKYLSLVYSVPKMEELSDSDNKDWLFSSNTSQVMKPKLEASE 533
               PQP+AIHKKSPHPDSK+LS+VYSVPKMEEL +SDN+DWLF+SNTSQ MKP+LEASE
Sbjct: 481 LQLQPQPTAIHKKSPHPDSKFLSVVYSVPKMEELLESDNQDWLFTSNTSQAMKPELEASE 540

BLAST of Lag0016385 vs. ExPASy TrEMBL
Match: A0A6J1KGW4 (DNA ligase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494242 PE=4 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.6e-196
Identity = 428/567 (75.49%), Postives = 464/567 (81.83%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEK+EGKEKRDKDKS+EKRR
Sbjct: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKKEGKEKRDKDKSEEKRR 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKA-HALEESRPLGKSGSQNGEEVVRAKHNSVQEE 120
           EKKDK KDRDKKK+RDKE    KEKEKA HALE+SR  GK  SQNGEEVVR KHNS+QEE
Sbjct: 61  EKKDKNKDRDKKKERDKE----KEKEKAPHALEDSRSSGKIKSQNGEEVVRIKHNSIQEE 120

Query: 121 KHAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKV---AIAQPKRDDGMA 180
           KH+G+ DSYVGNKISQN  L KETKNSKLVPE+GRRIEDSGTAKV   A+AQPKRDDG+A
Sbjct: 121 KHSGKLDSYVGNKISQNTVLPKETKNSKLVPEVGRRIEDSGTAKVEKLAVAQPKRDDGVA 180

Query: 181 TQVARSSETLFEG-KEQNKNKKIDERKYNGQGIRHEERFSGNSAVPSSTATTTATTTTTA 240
           TQVARSSE LFEG KE  KNK ID+ KYNGQGIRH+ERFSG+S VPS TATT        
Sbjct: 181 TQVARSSEILFEGNKEHIKNKTIDQGKYNGQGIRHDERFSGDSTVPSFTATT-------- 240

Query: 241 TATATATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDK 300
           T TA AT++S         EK  E+RKETNDKIR KEG+ KRG KHKDKDKEKKGRSNDK
Sbjct: 241 TTTANATVESA--------EKIAEKRKETNDKIRPKEGEAKRGSKHKDKDKEKKGRSNDK 300

Query: 301 ESDKEKKKEKKAKEK---------------EKVEEKNAMVDKTKEVNKDNLLVGRHSTNT 360
            +DKEKKKEKKAKEK               ++ EEKNA+VDKTKE+ KDN L+GRHST+T
Sbjct: 301 ATDKEKKKEKKAKEKKEKKAKEKKEKKAKQKEEEEKNAVVDKTKEITKDN-LIGRHSTDT 360

Query: 361 NTSQLPDSNISAVVEENLIKKRKDFEPNGVLHAIDNRSSKLLRHTSHPLKENGRILEPCL 420
           NTSQLPDSNI A VEENLIKKRK FEPNGVLHAIDNRSSKLLR  SHPLKENGRILEPCL
Sbjct: 361 NTSQLPDSNIGAAVEENLIKKRKAFEPNGVLHAIDNRSSKLLRPASHPLKENGRILEPCL 420

Query: 421 TSSLPPSDRQAV-ANDFLLGSKERKVNGIIEAHHPPASSKHTPGGQDDH----------- 480
            S LPPS+RQ V AND +L SKERK+NGIIEAHHPPAS KH    QDDH           
Sbjct: 421 ASPLPPSERQTVEANDVILFSKERKINGIIEAHHPPASLKHKTSEQDDHPQSQPQPQPQL 480

Query: 481 ---PQPSAIHKKSPHPDSKYLSLVYSVPKMEELSDSDNKDWLFSSNTSQVMKPKLEASEA 533
              PQP+AIHKKSPHPDSK+LS+VYSVPKMEELSDSDN+DWLF+SN SQ MKP+LEASEA
Sbjct: 481 QLQPQPTAIHKKSPHPDSKFLSVVYSVPKMEELSDSDNQDWLFTSNNSQAMKPELEASEA 540

BLAST of Lag0016385 vs. TAIR 10
Match: AT5G48610.1 (unknown protein; Has 43043 Blast hits to 23768 proteins in 1327 species: Archae - 108; Bacteria - 3952; Metazoa - 17076; Fungi - 3614; Plants - 1643; Viruses - 204; Other Eukaryotes - 16446 (source: NCBI BLink). )

HSP 1 Score: 51.6 bits (122), Expect = 2.3e-06
Identity = 122/383 (31.85%), Postives = 185/383 (48.30%), Query Frame = 0

Query: 1   MSRCFPFPPPGYEKKSRAEDADLLKKEKSKEKKHKKEKKEKEKREGKEKRDKDKSDEKRR 60
           MSRCFPFPPPGYEKK R ++AD L K+K KEKKH   KK+KEKREGKEK+ KD+S +K++
Sbjct: 1   MSRCFPFPPPGYEKKIRTDEADPLVKDKYKEKKH---KKDKEKREGKEKKSKDRSKDKQK 60

Query: 61  EKKDKKKDRDKKKDRDKEKDRDKEKEKAHALEESRPLGKSGSQNGEEVVRAKHNSVQEEK 120
           E+K+KK   DK KD   +KD++K KEK   LEE +                     +   
Sbjct: 61  ERKEKK---DKHKD---QKDKEKGKEKGKPLEEKK--------------------AELLT 120

Query: 121 HAGQFDSYVGNKISQNAFLSKETKNSKLVPELGRRIEDSGTAKVAIAQPKRDDGMATQVA 180
           +AG  ++ V + +  N+        SK V +L RRI              R D  AT  +
Sbjct: 121 NAGHRENRVTDTVQNNS-----NGESKYVQDLARRI--------------RYDEEATG-S 180

Query: 181 RSSETLFEGKEQNKNKKIDERKYNGQGIRH-EERFSGNSAVPSSTATTTATTTTTATATA 240
           +S++ +       KN  I E+ +    I     R   N  + +    T A ++  A +  
Sbjct: 181 QSAQRI--DHPNQKNVGITEKAFENSPIEETSHRVDDNKRINNQKNFTAAKSSENAVSRV 240

Query: 241 TATIQSGVAGMFKQLEKGTERRKETNDKIRQKEGDEKRGGKHKDKDKEKKGRSNDKESDK 300
           +        G   +  +   +  E  D++RQ E  EK    H+ ++  K  +  D+E  K
Sbjct: 241 S-------FGADHKRAEVMGKPMENRDQVRQTESAEK---SHRKENVTKSEKPRDQEGVK 300

Query: 301 EKKKEKKAKEKEKVEEKNAMVDKTKEVNKDNLLVG-------RHSTNTNTSQLPD---SN 360
           + + + K + KEK EEK   ++KT++  K  L+ G       + S +    +LPD   ++
Sbjct: 301 KTEAKDKDRNKEKKEEKTESINKTRQ-EKPKLIRGPKLEEREKDSPDLRNCKLPDVSRTS 321

Query: 361 ISAVVEENLIKKRKDFEPNGVLH 373
           I  +  E  + KRKD   NG L+
Sbjct: 361 IKNLHTEGNLGKRKDHMTNGFLY 321