Lag0016371 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016371
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H-like superfamily protein
Locationchr12: 36864002 .. 36864886 (+)
RNA-Seq ExpressionLag0016371
SyntenyLag0016371
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCTCCTTGTGTAAGAAATTTCCTGAGACTACTGACCATGCCTTAGTGACTTGCGCACGAGCGAAGCGGTTGTGGAAGACTTTACTGCCGCAGGTTGATTGGTCGCTGAACTTCAACAGTAGTTTTTGGGATCGATGTTTATTTTTACAAGGACTTTTGTCAGTTTCGGATTTTGGGTTGTGGTGTGTTGGTTGTTGGGCATGGTGGAATGATAGGAATGCGTATTTGAACGGTTCGATCCTCCCGGCTATCCCTGTTAAAGCTGAGTGGGTTGGCAAGTATTTGGGTTCCTTTTCCTCTGTGCATGAGAGTCGAAGGTCGGATATTTGTGGGTCTATTGTTCAAGGGCCAGTTTCAGTTTCGCAGTGGGTCTGTCCTCCCCAGGGCTGGTTCAAAATTAATGTTGATGCTTCCTGTTCATCAAAGTATAGTCTGACTGGCGTCGGCGTGGTTGTTCGAACGGCGTCGGGTCGTTTGCATGCTGCCCAATTGGAGGTCGTTCCTATTGTTCTTGCTCCTTTGCTCGCTGAAGCCCGTGCGGTACTCGTGGGTTTGAGGTTGGCACTGGTATTGGATCTCGTGTGTGTTGAGGTGGAATCAGATTGTCTTTCCCTCATTTCCATGTTGACTGGGTCCTTCATCTCCCTCCATGAAGAGGGTACTTATGTTGCTGAAATTCTTGAGTTGGCCTCTCACTTTACAAGTGTTGTTTTCCGTCACGTCCGCAGGGGAGGAAATAGGCCAGCGCACATTCTGGCCTCTCATGCTAGTGTTGAAGGTTCAATGTTGTGGTGTGCCTCTTTCCCTGAGTGGTTGACAGATGTTGTTGGTCAGGATTCTTCTCCTGCTAGTTGTAACCCTTGTGGTGATTTGATTCTCTAA

mRNA sequence

ATGTGCTCCTTGTGTAAGAAATTTCCTGAGACTACTGACCATGCCTTAGTGACTTGCGCACGAGCGAAGCGGTTGTGGAAGACTTTACTGCCGCAGGTTGATTGGTCGCTGAACTTCAACAGTAGTTTTTGGGATCGATGTTTATTTTTACAAGGACTTTTGTCAGTTTCGGATTTTGGGTTGTGGTGTGTTGGTTGTTGGGCATGGTGGAATGATAGGAATGCGTATTTGAACGGTTCGATCCTCCCGGCTATCCCTGTTAAAGCTGAGTGGGTTGGCAAGTATTTGGGTTCCTTTTCCTCTGTGCATGAGAGTCGAAGGTCGGATATTTGTGGGTCTATTGTTCAAGGGCCAGTTTCAGTTTCGCAGTGGGTCTGTCCTCCCCAGGGCTGGTTCAAAATTAATGTTGATGCTTCCTGTTCATCAAAGTATAGTCTGACTGGCGTCGGCGTGGTTGTTCGAACGGCGTCGGGTCGTTTGCATGCTGCCCAATTGGAGGTCGTTCCTATTGTTCTTGCTCCTTTGCTCGCTGAAGCCCGTGCGGTACTCGTGGGTTTGAGGTTGGCACTGGTATTGGATCTCGTGTGTGTTGAGGTGGAATCAGATTGTCTTTCCCTCATTTCCATGTTGACTGGGTCCTTCATCTCCCTCCATGAAGAGGGTACTTATGTTGCTGAAATTCTTGAGTTGGCCTCTCACTTTACAAGTGTTGTTTTCCGTCACGTCCGCAGGGGAGGAAATAGGCCAGCGCACATTCTGGCCTCTCATGCTAGTGTTGAAGGTTCAATGTTGTGGTGTGCCTCTTTCCCTGAGTGGTTGACAGATGTTGTTGGTCAGGATTCTTCTCCTGCTAGTTGTAACCCTTGTGGTGATTTGATTCTCTAA

Coding sequence (CDS)

ATGTGCTCCTTGTGTAAGAAATTTCCTGAGACTACTGACCATGCCTTAGTGACTTGCGCACGAGCGAAGCGGTTGTGGAAGACTTTACTGCCGCAGGTTGATTGGTCGCTGAACTTCAACAGTAGTTTTTGGGATCGATGTTTATTTTTACAAGGACTTTTGTCAGTTTCGGATTTTGGGTTGTGGTGTGTTGGTTGTTGGGCATGGTGGAATGATAGGAATGCGTATTTGAACGGTTCGATCCTCCCGGCTATCCCTGTTAAAGCTGAGTGGGTTGGCAAGTATTTGGGTTCCTTTTCCTCTGTGCATGAGAGTCGAAGGTCGGATATTTGTGGGTCTATTGTTCAAGGGCCAGTTTCAGTTTCGCAGTGGGTCTGTCCTCCCCAGGGCTGGTTCAAAATTAATGTTGATGCTTCCTGTTCATCAAAGTATAGTCTGACTGGCGTCGGCGTGGTTGTTCGAACGGCGTCGGGTCGTTTGCATGCTGCCCAATTGGAGGTCGTTCCTATTGTTCTTGCTCCTTTGCTCGCTGAAGCCCGTGCGGTACTCGTGGGTTTGAGGTTGGCACTGGTATTGGATCTCGTGTGTGTTGAGGTGGAATCAGATTGTCTTTCCCTCATTTCCATGTTGACTGGGTCCTTCATCTCCCTCCATGAAGAGGGTACTTATGTTGCTGAAATTCTTGAGTTGGCCTCTCACTTTACAAGTGTTGTTTTCCGTCACGTCCGCAGGGGAGGAAATAGGCCAGCGCACATTCTGGCCTCTCATGCTAGTGTTGAAGGTTCAATGTTGTGGTGTGCCTCTTTCCCTGAGTGGTTGACAGATGTTGTTGGTCAGGATTCTTCTCCTGCTAGTTGTAACCCTTGTGGTGATTTGATTCTCTAA

Protein sequence

MCSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGLWCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESRRSDICGSIVQGPVSVSQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEARAVLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFRHVRRGGNRPAHILASHASVEGSMLWCASFPEWLTDVVGQDSSPASCNPCGDLIL
Homology
BLAST of Lag0016371 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 136.3 bits (342), Expect = 4.1e-28
Identity = 96/282 (34.04%), Postives = 135/282 (47.87%), Query Frame = 0

Query: 2    CSLCKKFPETTDHALVTCARAKRLWKTLLPQVD-WSLNFNSSFWDRCLFLQGLLSVSDFG 61
            C++C    E+  HA   C RA+++W+TL P +   S   N SF +    L   L   D  
Sbjct: 1173 CTICGDRRESIIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLN 1232

Query: 62   LWCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESRRSDICGSIVQGPVS 121
            L  +  W  WNDRN+ ++G  +  +  K EW+  +L S S    S  S    S    PV 
Sbjct: 1233 LAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQS-NHRPV- 1292

Query: 122  VSQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEAR 181
            V  W        K+N DA+C  + + T  G ++R +S  L AA    VP  L+PLLAE R
Sbjct: 1293 VQYWRPSSSVSLKLNTDAAC--RGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIR 1352

Query: 182  AVLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFR 241
             +L GL+ A   +   +EVESD L  I ++     +  +E  +V EI  L   F  + F 
Sbjct: 1353 CILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFS 1412

Query: 242  HVRRGGNRPAHILASH--ASVEGSMLWCASFPEWLTDVVGQD 281
            H  R  NR AH LA     S   +  W  +FP WL D+V +D
Sbjct: 1413 HSSRQCNRAAHGLAKWGITSPSATYAWLFNFPTWLLDLVQRD 1450

BLAST of Lag0016371 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 124.0 bits (310), Expect = 2.1e-24
Identity = 73/189 (38.62%), Postives = 97/189 (51.32%), Query Frame = 0

Query: 2   CSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGL 61
           C +C K  ETTDHAL  C RAK +W  LLPQ     +FN+S  D  L+L   LS  DF L
Sbjct: 28  CLVCGKQIETTDHALFKCKRAKEVWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDL 87

Query: 62  WCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFS-----SVHESRRSDICGSIVQ 121
             VG WA WNDRNA      +P   ++++W+  Y+  F      S+ + R  +   S   
Sbjct: 88  VGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASS--N 147

Query: 122 GPVSVSQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLL 181
                + W  PP GW KINVDA+C      TG+G+V R   G++ AA          PL+
Sbjct: 148 DRTIENYWSPPPAGWIKINVDAACKKHQFRTGIGIVCRNEKGQILAAASH------DPLM 207

Query: 182 AEARAVLVG 186
           AE+ A+  G
Sbjct: 208 AESLALFDG 208

BLAST of Lag0016371 vs. NCBI nr
Match: XP_030497600.1 (uncharacterized protein LOC115713257 [Cannabis sativa])

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-24
Identity = 88/281 (31.32%), Postives = 132/281 (46.98%), Query Frame = 0

Query: 2    CSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGL 61
            CSLC +  E+  HAL +C  AK +W+    Q+D++        D  LFL  +L+ S+   
Sbjct: 1093 CSLCSRAWESIGHALFSCCHAKSVWQHTSFQLDFTKASFMKDGDYLLFLSTILTKSELEK 1152

Query: 62   WCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESRRSDICGSIVQGPVSV 121
                 W  W+DRN Y++   L      +     YL +F SV  +    +  S V      
Sbjct: 1153 LFCTMWFIWSDRNNYIHCKQLKHPMAISSQAEAYLANFHSVKSATAPAV--SCVAADART 1212

Query: 122  SQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEARA 181
             +WV P +   K+NVDA+  S  +  G+GV++R ++GR+ AA  + V         EA+A
Sbjct: 1213 VKWVPPTESNLKMNVDAALDSSRNKIGIGVIIRDSTGRVIAAMSKPVVGNFKSQEMEAKA 1272

Query: 182  VLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFRH 241
            +  GL+ A  L L    VE+DCL L+  L G    L      V +I    S F++    H
Sbjct: 1273 MFWGLQWAKQLQLQPHCVETDCLMLVHALQGKQSQLSSFHDLVEDISYHLSSFSNACISH 1332

Query: 242  VRRGGNRPAHILASHA-SVEGSMLWCASFPEWLTDVVGQDS 282
            VRR  N+ AH LA  A  ++   +W    P  +  VV  D+
Sbjct: 1333 VRRDANQAAHGLAKQALQLDNDCIWIEEIPSTIFSVVVNDT 1371

BLAST of Lag0016371 vs. NCBI nr
Match: XP_030502555.1 (uncharacterized protein LOC115717715 [Cannabis sativa])

HSP 1 Score: 121.7 bits (304), Expect = 1.1e-23
Identity = 87/282 (30.85%), Postives = 135/282 (47.87%), Query Frame = 0

Query: 2    CSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGL 61
            CSLC +  E+  HAL +C  AK +W+    Q+D+         D  +FL  +L+ S+   
Sbjct: 1093 CSLCSRVWESIGHALFSCCHAKAVWQNTGFQLDFQKASYMKDGDYLMFLSTILTNSELER 1152

Query: 62   WCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESRRSDICGSIVQGPVSV 121
                 W  W+DRN +++G  L      +     YL +F+SV    ++      V   V+ 
Sbjct: 1153 LFCTMWFIWSDRNNFIHGKQLKQPMAISSQAEAYLANFNSV--QLQATPATFRVAADVNR 1212

Query: 122  SQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEARA 181
             +W  PP+   K+NVDA+  S  S  G+GV++R +SG + AA  +           EA+A
Sbjct: 1213 VKWTPPPETKLKMNVDAAIDSSRSKIGMGVIIRDSSGSVVAAMSKPAVGNFKSQEMEAKA 1272

Query: 182  VLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFRH 241
            +  GL+LA  L L    VE+DCL L++ + G+          V +I    S  ++    H
Sbjct: 1273 MFWGLQLAEQLQLQLHYVETDCLMLVTAINGAQSQHSSFHDLVKDISYHLSSSSNACISH 1332

Query: 242  VRRGGNRPAHILASHA-SVEGSMLWCASFPEWLTDVVGQDSS 283
            VRR  N+ AH LA  A  ++   +W    P  +  VV +DSS
Sbjct: 1333 VRRDANQAAHGLAKQALQLDNDCIWIEEIPSSIFSVVVKDSS 1372

BLAST of Lag0016371 vs. NCBI nr
Match: XP_023880683.1 (uncharacterized protein LOC111993063 [Quercus suber])

HSP 1 Score: 118.6 bits (296), Expect = 8.9e-23
Identity = 85/287 (29.62%), Postives = 127/287 (44.25%), Query Frame = 0

Query: 1   MCSLCKKFPETTDHALVTCARAKRLW---KTLLPQVD-WSLNFNSSFWDRCLFLQGLLSV 60
           +C  C++ PET  H L +C +AK  W   K  +   D  +L F    W+  L L G    
Sbjct: 10  LCESCREAPETVGHVLWSCPKAKEAWECSKLAIGGFDGQNLTFQDLMWE--LLLNGEAGE 69

Query: 61  SDFGLWCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESR--RSDICGSI 120
                     WA W++RN    G    +    + W   YL  +++  E +  R  +    
Sbjct: 70  DKVAHAATTAWALWHNRNEIRCGGARKSGQQLSRWATDYLREYNAAAEVKIPRESV---- 129

Query: 121 VQGPVSVSQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAP 180
              P  V+ W  P  G FKINVD +  +     GVGVV+R   GRL AA    +   +  
Sbjct: 130 ---PRQVAFWSPPRNGHFKINVDGAVFAAQKAVGVGVVIRDDEGRLEAALCRKITAPMGA 189

Query: 181 LLAEARAVLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHF 240
           + AEA+A   GL  A  + +  + +E D L + + L           + V  I++++  F
Sbjct: 190 VEAEAKAFEAGLLFAKAVGVRDIILEGDSLVVYNALCNISSPPSSIASIVQGIVDMSGEF 249

Query: 241 TSVVFRHVRRGGNRPAHILASHAS-VEGSMLWCASFPEWLTDVVGQD 281
            SV + H+RR GN PAHILA HAS +   + W    P  +   +  D
Sbjct: 250 RSVSYSHIRRQGNVPAHILAKHASGITNYIAWIEEDPCCIAQALSHD 287

BLAST of Lag0016371 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.0e-28
Identity = 96/282 (34.04%), Postives = 135/282 (47.87%), Query Frame = 0

Query: 2    CSLCKKFPETTDHALVTCARAKRLWKTLLPQVD-WSLNFNSSFWDRCLFLQGLLSVSDFG 61
            C++C    E+  HA   C RA+++W+TL P +   S   N SF +    L   L   D  
Sbjct: 1173 CTICGDRRESIIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLN 1232

Query: 62   LWCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESRRSDICGSIVQGPVS 121
            L  +  W  WNDRN+ ++G  +  +  K EW+  +L S S    S  S    S    PV 
Sbjct: 1233 LAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQS-NHRPV- 1292

Query: 122  VSQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEAR 181
            V  W        K+N DA+C  + + T  G ++R +S  L AA    VP  L+PLLAE R
Sbjct: 1293 VQYWRPSSSVSLKLNTDAAC--RGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIR 1352

Query: 182  AVLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFR 241
             +L GL+ A   +   +EVESD L  I ++     +  +E  +V EI  L   F  + F 
Sbjct: 1353 CILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFS 1412

Query: 242  HVRRGGNRPAHILASH--ASVEGSMLWCASFPEWLTDVVGQD 281
            H  R  NR AH LA     S   +  W  +FP WL D+V +D
Sbjct: 1413 HSSRQCNRAAHGLAKWGITSPSATYAWLFNFPTWLLDLVQRD 1450

BLAST of Lag0016371 vs. ExPASy TrEMBL
Match: A0A803NGI9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 3.8e-27
Identity = 85/281 (30.25%), Postives = 135/281 (48.04%), Query Frame = 0

Query: 2    CSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGL 61
            CSLC    E+ +HAL  C  AK++W+     +++ L    SF D  + L  L S S+   
Sbjct: 847  CSLCSNAWESVEHALFLCKHAKKVWRVAGISLNFDLVSRMSFADFLMLLSTLKSTSEMEQ 906

Query: 62   WCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESRRSDICGSIVQGPVSV 121
                 W  WNDRN +++G   P +     W  K +  F +  +   S        G  + 
Sbjct: 907  LLCTLWFLWNDRNNFIHGK--PGLSPTQLW-AKSVAYFCNFQQQSTSSKSIRDSTGAAAQ 966

Query: 122  SQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEARA 181
              W+ PP    K+NVDA+C    +  GVG+++R +SG++ AA  + +   L P   EA+A
Sbjct: 967  PHWISPPLDKLKMNVDAACDISRNKIGVGIIIRNSSGQVVAAYSKPLTGRLKPQEMEAKA 1026

Query: 182  VLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFRH 241
            +L+G+  A   +L     ESD L L++ +     ++   G  V +I    S+ +SV   H
Sbjct: 1027 LLIGINWAARCNLSINLFESDSLILVNSINSISNAISSFGDLVLDIKNRLSYLSSVCVSH 1086

Query: 242  VRRGGNRPAHILASHA-SVEGSMLWCASFPEWLTDVVGQDS 282
            V+R  N+ AH LA HA  ++   +W    P  +  VV  D+
Sbjct: 1087 VKRDANQAAHGLAKHALELDDDCMWFEEIPSTIFSVVVNDA 1124

BLAST of Lag0016371 vs. ExPASy TrEMBL
Match: A0A6J1CTE3 (uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014578 PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-24
Identity = 73/189 (38.62%), Postives = 97/189 (51.32%), Query Frame = 0

Query: 2   CSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGL 61
           C +C K  ETTDHAL  C RAK +W  LLPQ     +FN+S  D  L+L   LS  DF L
Sbjct: 28  CLVCGKQIETTDHALFKCKRAKEVWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDL 87

Query: 62  WCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFS-----SVHESRRSDICGSIVQ 121
             VG WA WNDRNA      +P   ++++W+  Y+  F      S+ + R  +   S   
Sbjct: 88  VGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASS--N 147

Query: 122 GPVSVSQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLL 181
                + W  PP GW KINVDA+C      TG+G+V R   G++ AA          PL+
Sbjct: 148 DRTIENYWSPPPAGWIKINVDAACKKHQFRTGIGIVCRNEKGQILAAASH------DPLM 207

Query: 182 AEARAVLVG 186
           AE+ A+  G
Sbjct: 208 AESLALFDG 208

BLAST of Lag0016371 vs. ExPASy TrEMBL
Match: A0A803PIB6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.8e-24
Identity = 88/281 (31.32%), Postives = 132/281 (46.98%), Query Frame = 0

Query: 2    CSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGL 61
            CSLC +  E+  HAL +C  AK +W+    Q+D++        D  LFL  +L+ S+   
Sbjct: 1553 CSLCSRAWESIGHALFSCCHAKSVWQHTSFQLDFTKASFMKDGDYLLFLSTILTKSELEK 1612

Query: 62   WCVGCWAWWNDRNAYLNGSILPAIPVKAEWVGKYLGSFSSVHESRRSDICGSIVQGPVSV 121
                 W  W+DRN Y++   L      +     YL +F SV  +    +  S V      
Sbjct: 1613 LFCTMWFIWSDRNNYIHCKQLKHPMAISSQAEAYLANFHSVKSATAPAV--SCVAADART 1672

Query: 122  SQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEARA 181
             +WV P +   K+NVDA+  S  +  G+GV++R ++GR+ AA  + V         EA+A
Sbjct: 1673 VKWVPPTESNLKMNVDAALDSSRNKIGIGVIIRDSTGRVIAAMSKPVVGNFKSQEMEAKA 1732

Query: 182  VLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFRH 241
            +  GL+ A  L L    VE+DCL L+  L G    L      V +I    S F++    H
Sbjct: 1733 MFWGLQWAKQLQLQPHCVETDCLMLVHALQGKQSQLSSFHDLVEDISYHLSSFSNACISH 1792

Query: 242  VRRGGNRPAHILASHA-SVEGSMLWCASFPEWLTDVVGQDS 282
            VRR  N+ AH LA  A  ++   +W    P  +  VV  D+
Sbjct: 1793 VRRDANQAAHGLAKQALQLDNDCIWIEEIPSTIFSVVVNDT 1831

BLAST of Lag0016371 vs. ExPASy TrEMBL
Match: A0A803NI87 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 9.6e-23
Identity = 80/260 (30.77%), Postives = 124/260 (47.69%), Query Frame = 0

Query: 2    CSLCKKFPETTDHALVTCARAKRLWKTLLPQVDWSLNFNSSFWDRCLFLQGLLSVSDFGL 61
            CSLC    E+  HAL+ C  AK++W +    +D+ +     F D  + L  L + S+   
Sbjct: 754  CSLCSNAWESIKHALLLCKHAKKVWHSAGYSLDYDMLARMKFSDCLVLLSTLKTTSEMEQ 813

Query: 62   WCVGCWAWWNDRNAYLNGSILPAIPVKAEWV--GKYLGSFSSVHESRRSDICGSIVQGPV 121
                 W  W DRN Y++G   P +     W     Y  +F      + S +   I  G  
Sbjct: 814  MFCTMWFLWTDRNNYIHGK--PVLSPSQIWAKSAAYYCNFQHHSTIQNSPM---ISTGAA 873

Query: 122  SVSQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASGRLHAAQLEVVPIVLAPLLAEA 181
            + S+W+ PP    K+NVDA+C    +  GVG++VR +SG++ AA  +           EA
Sbjct: 874  TQSKWIRPPLNELKMNVDAACDVSRNKIGVGIIVRNSSGQIVAAYSKSFTGRYKSQEMEA 933

Query: 182  RAVLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVF 241
            +A+L GL  A   +L+   +ESD L L++ +     ++   G  V +I    S+ +SV  
Sbjct: 934  KALLTGLDWAAHHNLLVSTLESDSLVLVNSINSQSTAISSFGDLVLDIKNRLSYLSSVCV 993

Query: 242  RHVRRGGNRPAHILASHASV 260
             HV+R  N+ AH LA  A V
Sbjct: 994  SHVKRDANQAAHGLAKQALV 1008

BLAST of Lag0016371 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-12
Identity = 77/300 (25.67%), Postives = 128/300 (42.67%), Query Frame = 0

Query: 2   CSLCKKFPETTDHALVTCARAKRLWK----TLLPQVDWSLNFNSSFWDRCLFLQGLLSVS 61
           C  C +  E+ +HAL TC  A   W+    +L+     S +F  +  +   F+Q   ++S
Sbjct: 200 CPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQD-TTMS 259

Query: 62  DF----GLWCVGCWAWWNDRNAYL--------NGSILPAIPVKAEWVGKYLGSFSSVHES 121
           DF     +W +  W  W  RN  +        + ++L A     +W+        +   +
Sbjct: 260 DFHKLLPVWLI--WRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQSHKKTPSPT 319

Query: 122 RRSDICGSIVQGPVSVSQWVCPPQGWFKINVDASCS-SKYSLTGVGVVVRTASGRLHAAQ 181
           R+      I +  +   +W  PP  + K N DA     K   TG G ++R   G   +  
Sbjct: 320 RQ------IAENKI---EWRNPPATYVKCNFDAGFDVQKLEATG-GWIIRNHYGTPISWG 379

Query: 182 LEVVPIVLAPLLAEARAVLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEE-GTY 241
              +     PL AE +A+L  L+   +     V +E DC +LI+++ G  IS H     +
Sbjct: 380 SMKLAHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLING--ISFHSSLANH 439

Query: 242 VAEILELASHFTSVVFRHVRRGGNRPAHILASHASVEGSML-WCASFPEWLTDVVGQDSS 283
           + +I   A+ F S+ F  +RR GN+ AH+LA +     +      S P WL      DS+
Sbjct: 440 LEDISFWANKFASIQFGFIRRKGNKLAHVLAKYGCTYSTFYSGSGSLPIWLDRYFCNDSN 484

BLAST of Lag0016371 vs. TAIR 10
Match: AT2G13980.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.8e-05
Identity = 41/134 (30.60%), Postives = 67/134 (50.00%), Query Frame = 0

Query: 122 SQWVCPPQGWFKINVDASCSSKYSLTGVGVVVRTASG-RLHAAQLEVVPIVLAPLLAEAR 181
           ++W  PP    K N DA  + +   +    ++R   G   H   L+ +     PL AE +
Sbjct: 11  TKWKAPPDTHIKCNYDAGFNVQNLDSTARWIIRNHDGIAQHWGSLQ-LDNTSTPLEAETK 70

Query: 182 AVLVGLRLALVLDLVCVEVESDCLSLISMLTGSFISLHEEGTYVAEILELASHFTSVVFR 241
           A+L  L+   +   + V +E DC +L ++++GS  S +     + +I   A  F++V F 
Sbjct: 71  ALLAALQQTWIRGYLRVIMEGDCETLTNLVSGS-SSHNHLANLLDDIRIWAKKFSNVQFS 130

Query: 242 HVRRGGNRPAHILA 255
            VRRGGN+ AH LA
Sbjct: 131 FVRRGGNKVAHELA 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.14.1e-2834.04uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_022145060.12.1e-2438.62uncharacterized protein LOC111014578 [Momordica charantia][more]
XP_030497600.13.6e-2431.32uncharacterized protein LOC115713257 [Cannabis sativa][more]
XP_030502555.11.1e-2330.85uncharacterized protein LOC115717715 [Cannabis sativa][more]
XP_023880683.18.9e-2329.62uncharacterized protein LOC111993063 [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DX302.0e-2834.04uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A803NGI93.8e-2730.25Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1CTE31.0e-2438.62uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A803PIB61.8e-2431.32Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803NI879.6e-2330.77Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G09510.11.2e-1225.67Ribonuclease H-like superfamily protein [more]
AT2G13980.11.8e-0530.60Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 135..257
e-value: 1.0E-22
score: 80.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 131..258
e-value: 1.3E-15
score: 59.6
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 119..281
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 134..254
e-value: 5.39504E-22
score: 86.9844
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 130..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016371.1Lag0016371.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity