Lag0016368 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016368
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionBHLH transcription factor
Locationchr12: 36833477 .. 36836619 (-)
RNA-Seq ExpressionLag0016368
SyntenyLag0016368
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAAGCAGAGCCTTCGTACAGAAAGATGTATGAAGAAACTGCTTGTTCTGACCCCAATTCCATGTCAGCCGAAGCTACACCGCCTGTACGAGATGCTTCAGAAAATGGGTTTCCTCCTACAATGGCGCATATTGCCACTTTGCCTTCTTTTCCTCCGCAGCTCATCGCCGGTGGTGCTGGTCAAGAAGCCCATGCCAATTTTGATGAGAATCTCAATCTTTCCATGGAGGAGCTGTCTTATCACAACAACCACCGCGGCGGCAGTGGAGACGCCATGGAACTTGAGTTTCAGCAGCAGCAGGCGGCCGCTGCAGCGGCAGTGGCGGGGTTTGATAACGGTAATGTTAACCCTAATTTTGGTCAGGAAGTAACTTCTGATTCGAACAGGATGGTGTGTTTTGATCAATCCAGTTGGGTTGGGACACAAATTCAAGAAATGGGGTTTAATCACCACCACCATCAGATTCAATCTCAAGATCAGTTTTCAGATTCCGCCATGGCCGCGGCCTATACTCAAGCTCCTGATCTCTTGAACTTCTTCAACATGCCGGCGGCCAGATGCTCAAACTCTTCCATCTCCTTCTCCAATACGATCTCGAACCTCCACAGTTCCGCCATGGGAGGGTTTCTTGCAGACCTTCCCGCCGGCGACGGCGGTGGAAACCCATCGTCGACCTCGCTTTCTATACTCTACGACCCTCTGTTCCACCTGAATCTGCCGCCGCAGCCGCCATTGTTTAGGGAGCTGTTTCACTCTCTTCCACATGGGTACAGTCTGCCGTCGGCCAGTTCCAGAGGCCGGGGAGGGAGTCTGTTCCCGGAGGGAGAGATGGAGAGAGAAGGAACCGCCGGAGTTTATGAAGACGGCGATGGAAGTGGAGTTTTGGAGTTCAGCAGGGATATGGCGGACTGTATTGGGAAAGGGAGAGATGGGAAAATGACTAAACATTCGCCCATTGAACGCCAGAGGAGAGTGCAATTGAATGAGAAATATAAAGCCCTGAGGAGTTTGGTTCCAAATCCTACTAAGGTTTTTGCCATTTCTTAATTAGATCACCATTTCTTAACTTTGATTTGCCTTCTGGGTGTGATTGAACTGCCAATTTTGTTAGTCTTTCTTTAGTATTTCATTTGATCATGAATATAAGATAATGGTGAGTTCACTTTTTTGGAATTTAATTTACTTTGTGCGTGTGACTGAATCAAGCTTTCTATCTCTGAACTTCCATTTTTATTGCTCTATTTCCTTTGATCTTGAATAACAAACTAGACTGATTGGTTCGGTACTATTTTTTAACTTTAATTTACTTTGTGGGTGTGGCTGAATCAAGATTTCATTAAGTTCAAACCCACTATTTTTTTTTTTCTTTTTCCTCTGATAAGTTCTCTATTTTTGAACTTTGATTTGCTTTCTGAAGCTCTCTAGTCTCTATCTAGCTCAACTTTTTTTTCTCTCTCCTTTGATCTTGAATTACAAACTACAAACTAAACTGATAACTAGACTGATAGTTTCACCATTTTTTTCTATCTTCTGCCTTTTGATCTCAACAAACTAGCTAGACTGATTGGTTTACCATTTTTTTAACTTAATTTGGTTTCTGGGTGTGACCTAATCAAGCTTTCTCTCTCTAACCCACCTTTTTTTTGTGTTCCTTTTTCCTTTGATTTTAAGCAACAAACTTGACTGATAGGTTCACCATTTTTGTTGGGTGTGACTGCATCGCCAGTTTTTTTTTTTCTCTTTCCTTCCTTGAATCTCAAACATGAAACAACAAACTAGAGTGAAGAATCACCATTTTATTAATATACACTTCAGTTCTCTTTCTGGGTTGTGACCAAATCGAGCTCTCTCTCTCTCCAACCCACCATTTTCTCTCTCTTCTGATCTCAAACAACAAATAAACTAGACTGGTTCATCACTTTTAAACTTTATTTGCTTTCTGGGTTTACTAAATCAAACCTTCTCTCTTTAACCCACCATTTTTTTTCTCTCCATCCTTTGATCTCACACAAACAAACCAGACTGATAGGGCATCAATTGTGGGAGACGCCATAGAATACATTAAAGAGCTTCTGAGAACAGTGAAGGAACTGAAACTACTGGTGGAGAAGAAGAGATGCAGCAGAGAGAGGAGCAAGAGGCACCGGACGGCGGAGGAAGACGGCGGCGCGTGGGATGTAGAGAGCTCAAATGCGAAGCCAGCTGTTGTGGGAGATGCCGCAGAGGATCAGAGCTACAACTTGAGAAGCTCGTGGCTGCAGAGGAAGACCAAAGACACTGAAGTTGATGTTCGGATCATTGACGATGAAGTGACCATCAAGCTTGTGCAGCGCAAAGTCAACTGCTTGTTGCTTGTCTCCAAATTGCTAGACGATCTTCAGCTTGATCTTCACCATGTCGCCGGTGGCCATATCGGTGATTACTACAGTTTCTTGTTCAATACCAAGGTTTGTCCCAAACTATGATTTTTTTTTTCTTCTTTTAATATGATGGATGATTTTTCTTTTTCCACTTTCAAACCTTCCACTTTGGGATCCTTTGGTTGGGAACTCGAGTTTGACATCTTAAGCTCGAGTTTAAGCTCTATGGTTTAAATGAAAGTTTAAGAGAATTTGAATCTGGAAAAAATAGATTTGAGCTGGTAAGAAAACACTTTTATCGTTTGTGTGTTAGGAATGCGACCAAAGTGAAGCATTTGAATGAGAAATCTTTGAATCTTGAAACTAAGAAAACGAGAGGTAGAAAAAGAGAAATCTTTTTTAACTTTCCTAATTATTGTCAACATTTTAATGGTGGTGTGTAGGACAATCTCTGTATATATGGGTATGCAATTAAGTATTCTTCAATTTCATTTTGATGGGACTCCATGAAATACTTTTAAATTTTCTTTGATAGTTTACTTTCCTAATTTTAGTTACTATAAACATATTTGACAAATGCGACCAAAGTGAAGTATTTGAATGAGTTAGTGAACATAAAAGTATATTTTCATTTTCATTTTCATTTTGATTTTGATTTTCCTTGATCAGATATATGAAGGTTCATCAGTGTATGCAAGTGCCATAGCCAACAGAGTGATAGAAGCAGTAGACAGACAGTACAACAACAACATATCCCACTACTCCACAGAGCAATAG

mRNA sequence

ATGGCGGAAGCAGAGCCTTCGTACAGAAAGATGTATGAAGAAACTGCTTGTTCTGACCCCAATTCCATGTCAGCCGAAGCTACACCGCCTGTACGAGATGCTTCAGAAAATGGGTTTCCTCCTACAATGGCGCATATTGCCACTTTGCCTTCTTTTCCTCCGCAGCTCATCGCCGGTGGTGCTGGTCAAGAAGCCCATGCCAATTTTGATGAGAATCTCAATCTTTCCATGGAGGAGCTGTCTTATCACAACAACCACCGCGGCGGCAGTGGAGACGCCATGGAACTTGAGTTTCAGCAGCAGCAGGCGGCCGCTGCAGCGGCAGTGGCGGGGTTTGATAACGGTAATGTTAACCCTAATTTTGGTCAGGAAGTAACTTCTGATTCGAACAGGATGGTGTGTTTTGATCAATCCAGTTGGGTTGGGACACAAATTCAAGAAATGGGGTTTAATCACCACCACCATCAGATTCAATCTCAAGATCAGTTTTCAGATTCCGCCATGGCCGCGGCCTATACTCAAGCTCCTGATCTCTTGAACTTCTTCAACATGCCGGCGGCCAGATGCTCAAACTCTTCCATCTCCTTCTCCAATACGATCTCGAACCTCCACAGTTCCGCCATGGGAGGGTTTCTTGCAGACCTTCCCGCCGGCGACGGCGGTGGAAACCCATCGTCGACCTCGCTTTCTATACTCTACGACCCTCTGTTCCACCTGAATCTGCCGCCGCAGCCGCCATTGTTTAGGGAGCTGTTTCACTCTCTTCCACATGGGTACAGTCTGCCGTCGGCCAGTTCCAGAGGCCGGGGAGGGAGTCTGTTCCCGGAGGGAGAGATGGAGAGAGAAGGAACCGCCGGAGTTTATGAAGACGGCGATGGAAGTGGAGTTTTGGAGTTCAGCAGGGATATGGCGGACTGTATTGGGAAAGGGAGAGATGGGAAAATGACTAAACATTCGCCCATTGAACGCCAGAGGAGAGTGCAATTGAATGAGAAATATAAAGCCCTGAGGAGTTTGGTTCCAAATCCTACTAAGACTGATAGGGCATCAATTGTGGGAGACGCCATAGAATACATTAAAGAGCTTCTGAGAACAGTGAAGGAACTGAAACTACTGGTGGAGAAGAAGAGATGCAGCAGAGAGAGGAGCAAGAGGCACCGGACGGCGGAGGAAGACGGCGGCGCGTGGGATGTAGAGAGCTCAAATGCGAAGCCAGCTGTTGTGGGAGATGCCGCAGAGGATCAGAGCTACAACTTGAGAAGCTCGTGGCTGCAGAGGAAGACCAAAGACACTGAAGTTGATGTTCGGATCATTGACGATGAAGTGACCATCAAGCTTGTGCAGCGCAAAGTCAACTGCTTGTTGCTTGTCTCCAAATTGCTAGACGATCTTCAGCTTGATCTTCACCATGTCGCCGGTGGCCATATCGGTGATTACTACAGTTTCTTGTTCAATACCAAGATATATGAAGGTTCATCAGTGTATGCAAGTGCCATAGCCAACAGAGTGATAGAAGCAGTAGACAGACAGTACAACAACAACATATCCCACTACTCCACAGAGCAATAG

Coding sequence (CDS)

ATGGCGGAAGCAGAGCCTTCGTACAGAAAGATGTATGAAGAAACTGCTTGTTCTGACCCCAATTCCATGTCAGCCGAAGCTACACCGCCTGTACGAGATGCTTCAGAAAATGGGTTTCCTCCTACAATGGCGCATATTGCCACTTTGCCTTCTTTTCCTCCGCAGCTCATCGCCGGTGGTGCTGGTCAAGAAGCCCATGCCAATTTTGATGAGAATCTCAATCTTTCCATGGAGGAGCTGTCTTATCACAACAACCACCGCGGCGGCAGTGGAGACGCCATGGAACTTGAGTTTCAGCAGCAGCAGGCGGCCGCTGCAGCGGCAGTGGCGGGGTTTGATAACGGTAATGTTAACCCTAATTTTGGTCAGGAAGTAACTTCTGATTCGAACAGGATGGTGTGTTTTGATCAATCCAGTTGGGTTGGGACACAAATTCAAGAAATGGGGTTTAATCACCACCACCATCAGATTCAATCTCAAGATCAGTTTTCAGATTCCGCCATGGCCGCGGCCTATACTCAAGCTCCTGATCTCTTGAACTTCTTCAACATGCCGGCGGCCAGATGCTCAAACTCTTCCATCTCCTTCTCCAATACGATCTCGAACCTCCACAGTTCCGCCATGGGAGGGTTTCTTGCAGACCTTCCCGCCGGCGACGGCGGTGGAAACCCATCGTCGACCTCGCTTTCTATACTCTACGACCCTCTGTTCCACCTGAATCTGCCGCCGCAGCCGCCATTGTTTAGGGAGCTGTTTCACTCTCTTCCACATGGGTACAGTCTGCCGTCGGCCAGTTCCAGAGGCCGGGGAGGGAGTCTGTTCCCGGAGGGAGAGATGGAGAGAGAAGGAACCGCCGGAGTTTATGAAGACGGCGATGGAAGTGGAGTTTTGGAGTTCAGCAGGGATATGGCGGACTGTATTGGGAAAGGGAGAGATGGGAAAATGACTAAACATTCGCCCATTGAACGCCAGAGGAGAGTGCAATTGAATGAGAAATATAAAGCCCTGAGGAGTTTGGTTCCAAATCCTACTAAGACTGATAGGGCATCAATTGTGGGAGACGCCATAGAATACATTAAAGAGCTTCTGAGAACAGTGAAGGAACTGAAACTACTGGTGGAGAAGAAGAGATGCAGCAGAGAGAGGAGCAAGAGGCACCGGACGGCGGAGGAAGACGGCGGCGCGTGGGATGTAGAGAGCTCAAATGCGAAGCCAGCTGTTGTGGGAGATGCCGCAGAGGATCAGAGCTACAACTTGAGAAGCTCGTGGCTGCAGAGGAAGACCAAAGACACTGAAGTTGATGTTCGGATCATTGACGATGAAGTGACCATCAAGCTTGTGCAGCGCAAAGTCAACTGCTTGTTGCTTGTCTCCAAATTGCTAGACGATCTTCAGCTTGATCTTCACCATGTCGCCGGTGGCCATATCGGTGATTACTACAGTTTCTTGTTCAATACCAAGATATATGAAGGTTCATCAGTGTATGCAAGTGCCATAGCCAACAGAGTGATAGAAGCAGTAGACAGACAGTACAACAACAACATATCCCACTACTCCACAGAGCAATAG

Protein sequence

MAEAEPSYRKMYEETACSDPNSMSAEATPPVRDASENGFPPTMAHIATLPSFPPQLIAGGAGQEAHANFDENLNLSMEELSYHNNHRGGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEVTSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQDQFSDSAMAAAYTQAPDLLNFFNMPAARCSNSSISFSNTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGEMEREGTAGVYEDGDGSGVLEFSRDMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQYNNNISHYSTEQ
Homology
BLAST of Lag0016368 vs. NCBI nr
Match: XP_023006449.1 (transcription factor bHLH91-like [Cucurbita maxima] >XP_023006450.1 transcription factor bHLH91-like [Cucurbita maxima])

HSP 1 Score: 842.0 bits (2174), Expect = 2.7e-240
Identity = 450/510 (88.24%), Postives = 467/510 (91.57%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPP-VRDASENGFPPTMAHIATLPSFPPQLIAGGAGQEAHANF 70
           MYEETACSDPNSMSAE TPP VRDASENGFP TMAHI TLPSFPP LI GG GQ++HANF
Sbjct: 1   MYEETACSDPNSMSAEPTPPGVRDASENGFPQTMAHIPTLPSFPPPLIVGG-GQDSHANF 60

Query: 71  DE-NLNLSMEELSYHNNHR-GGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEVTS 130
           D+ NLNLSMEE+SYH++HR GG GDAMELEFQQQ         GFDN N+NP+FGQEV+S
Sbjct: 61  DDNNLNLSMEEISYHHHHRSGGGGDAMELEFQQQ-------AVGFDNCNMNPSFGQEVSS 120

Query: 131 DSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQD-QFSDSAMAAAYTQAPDLLNFFNMPA 190
           DSNRMVC DQS+W GTQIQEMGFN  HH IQSQD QFSDSAMAAAYTQAPDLLNFFNMPA
Sbjct: 121 DSNRMVCLDQSAWAGTQIQEMGFN--HHLIQSQDHQFSDSAMAAAYTQAPDLLNFFNMPA 180

Query: 191 ARCS-NSSISFS-NTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQ 250
           ARCS NSSISFS NTISNLHSSAMGGFLADLPAGD G NPSSTSLSILYDPLFHLNLPPQ
Sbjct: 181 ARCSNNSSISFSNNTISNLHSSAMGGFLADLPAGD-GSNPSSTSLSILYDPLFHLNLPPQ 240

Query: 251 PPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE-MEREGTAGVYEDGDGSGVLEFSRDM 310
           PPLFRELFHSLPHGY LP+ASSRGRGGSLFPEGE MEREGTAGVYEDGDGSGVLEFSRDM
Sbjct: 241 PPLFRELFHSLPHGYGLPAASSRGRGGSLFPEGEIMEREGTAGVYEDGDGSGVLEFSRDM 300

Query: 311 ADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELL 370
           ADCIGKGRDGKMTKH   ERQRRVQLN+KYKALRSLVP+PTKTDRASIVGDAI YIKELL
Sbjct: 301 ADCIGKGRDGKMTKHFATERQRRVQLNDKYKALRSLVPSPTKTDRASIVGDAINYIKELL 360

Query: 371 RTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYNLRSSW 430
           R VKELKLLVEKKRCSRERSKRHRTAEEDG AWDVESSNAK   VGD  EDQSYNLRSSW
Sbjct: 361 REVKELKLLVEKKRCSRERSKRHRTAEEDGSAWDVESSNAKTVAVGDGGEDQSYNLRSSW 420

Query: 431 LQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFL 490
           LQRKTKDTEVDVRI+DDEVTIKLVQRK+NCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFL
Sbjct: 421 LQRKTKDTEVDVRIVDDEVTIKLVQRKLNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFL 480

Query: 491 FNTKIYEGSSVYASAIANRVIEAVDRQYNN 514
           FNTKIYEGSSVYASAIAN+V+EAVDRQYNN
Sbjct: 481 FNTKIYEGSSVYASAIANKVMEAVDRQYNN 499

BLAST of Lag0016368 vs. NCBI nr
Match: XP_022958755.1 (transcription factor bHLH91-like [Cucurbita moschata] >XP_022958756.1 transcription factor bHLH91-like [Cucurbita moschata])

HSP 1 Score: 829.7 bits (2142), Expect = 1.4e-236
Identity = 447/512 (87.30%), Postives = 465/512 (90.82%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPP-VRDASENGFPPTMAHIATLPSF--PPQLIAGGAGQEAHA 70
           MYEETACSDPNSMSAE TPP V DAS+NGFP TMAHI  LPSF  PP LI  G GQ+ HA
Sbjct: 1   MYEETACSDPNSMSAEPTPPVVPDASQNGFPQTMAHIPALPSFPPPPPLIVAG-GQDTHA 60

Query: 71  NFDE-NLNLSMEELSYHNNHR-GGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEV 130
           NFD+ NLNLSMEE+SYH++HR GG GDAMELEFQQQ         GFDN N+NP+FGQEV
Sbjct: 61  NFDDNNLNLSMEEISYHHHHRSGGGGDAMELEFQQQG-------VGFDNCNMNPSFGQEV 120

Query: 131 TSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQD-QFSDSAMAAAYTQAPDLLNFFNM 190
           +SDSNRMVC DQS+WVG+QIQEMGFN  HH IQSQD QFSDSAMAAAYTQAPDLLNFFNM
Sbjct: 121 SSDSNRMVCLDQSAWVGSQIQEMGFN--HHLIQSQDHQFSDSAMAAAYTQAPDLLNFFNM 180

Query: 191 PAARCS-NSSISFS-NTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLP 250
           PAARCS NSSISFS NTISNLHSSAMGGFLADLPAGD G NPSSTSLSILYDPLFHLNLP
Sbjct: 181 PAARCSNNSSISFSNNTISNLHSSAMGGFLADLPAGD-GSNPSSTSLSILYDPLFHLNLP 240

Query: 251 PQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE-MEREGTAGVYEDGDGSGVLEFSR 310
           PQPPLFRELFHSLPHGY LP+ASSRGRGGSLFPEGE MEREGTAGVYEDGDGSGVLEFSR
Sbjct: 241 PQPPLFRELFHSLPHGYGLPAASSRGRGGSLFPEGEIMEREGTAGVYEDGDGSGVLEFSR 300

Query: 311 DMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKE 370
           DMADCIGKGRDGKMTKH   ERQRRVQLN+KYKALRSLVP+PTKTDRASIVGDAI YIKE
Sbjct: 301 DMADCIGKGRDGKMTKHFATERQRRVQLNDKYKALRSLVPSPTKTDRASIVGDAINYIKE 360

Query: 371 LLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYNLRS 430
           LLR VKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAK   VGD  EDQSYNLRS
Sbjct: 361 LLREVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKTGAVGDGGEDQSYNLRS 420

Query: 431 SWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYS 490
           SWLQRKTKDTEVDVRI+DDEVTIKLVQRK+NCLLLVSKLLDDLQLDLHHVAGGHIGDYYS
Sbjct: 421 SWLQRKTKDTEVDVRIVDDEVTIKLVQRKLNCLLLVSKLLDDLQLDLHHVAGGHIGDYYS 480

Query: 491 FLFNTKIYEGSSVYASAIANRVIEAVDRQYNN 514
           FLFNTKIYEGSSVYASAIAN+V+EAVDRQYNN
Sbjct: 481 FLFNTKIYEGSSVYASAIANKVMEAVDRQYNN 501

BLAST of Lag0016368 vs. NCBI nr
Match: KAG7013409.1 (Transcription factor bHLH91 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 826.2 bits (2133), Expect = 1.5e-235
Identity = 446/515 (86.60%), Postives = 464/515 (90.10%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPP-VRDASENGFPPTMAHIATLPSF--PPQLIAGGAGQEAHA 70
           MYEETACSDPNSMSAE TPP V DAS+NGFP TMAHI  LPSF  PP LI  G GQ+ HA
Sbjct: 1   MYEETACSDPNSMSAEPTPPVVPDASQNGFPQTMAHIPALPSFSPPPPLIVAG-GQDTHA 60

Query: 71  NFDE-NLNLSMEELSY----HNNHRGGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFG 130
           NFD+ NLNLSMEE+SY    H++HR G GDAMELEFQQQ         GFDN N+NP+FG
Sbjct: 61  NFDDNNLNLSMEEISYHHHHHHHHRSGGGDAMELEFQQQG-------VGFDNCNMNPSFG 120

Query: 131 QEVTSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQD-QFSDSAMAAAYTQAPDLLNF 190
           QEV+SDSNRMVC DQS+WVG+QIQEMGFN  HH IQSQD QFSDSAMAAAYTQAPDLLNF
Sbjct: 121 QEVSSDSNRMVCLDQSAWVGSQIQEMGFN--HHLIQSQDHQFSDSAMAAAYTQAPDLLNF 180

Query: 191 FNMPAARCS-NSSISFS-NTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHL 250
           FNMPAARCS NSSISFS NTISNLHSSAMGGFLADLPAGD G NPSSTSLSILYDPLFHL
Sbjct: 181 FNMPAARCSNNSSISFSNNTISNLHSSAMGGFLADLPAGD-GSNPSSTSLSILYDPLFHL 240

Query: 251 NLPPQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE-MEREGTAGVYEDGDGSGVLE 310
           NLPPQPPLFRELFHSLPHGY LP+ASSRGRGGSLFPEGE MEREGTAGVYEDGDGSGVLE
Sbjct: 241 NLPPQPPLFRELFHSLPHGYGLPAASSRGRGGSLFPEGEIMEREGTAGVYEDGDGSGVLE 300

Query: 311 FSRDMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEY 370
           FSRDMADCIGKGRDGKMTKH   ERQRRVQLN+KYKALRSLVP+PTKTDRASIVGDAI Y
Sbjct: 301 FSRDMADCIGKGRDGKMTKHFATERQRRVQLNDKYKALRSLVPSPTKTDRASIVGDAINY 360

Query: 371 IKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYN 430
           IKELLR VKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAK   VGD  EDQSYN
Sbjct: 361 IKELLREVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKTGAVGDGGEDQSYN 420

Query: 431 LRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGD 490
           LRSSWLQRKTKDTEVDVRI+DDEVTIKLVQRK+NCLLLVSKLLDDLQLDLHHVAGGHIGD
Sbjct: 421 LRSSWLQRKTKDTEVDVRIVDDEVTIKLVQRKLNCLLLVSKLLDDLQLDLHHVAGGHIGD 480

Query: 491 YYSFLFNTKIYEGSSVYASAIANRVIEAVDRQYNN 514
           YYSFLFNTKIYEGSSVYASAIAN+V+EAVDRQYNN
Sbjct: 481 YYSFLFNTKIYEGSSVYASAIANKVMEAVDRQYNN 504

BLAST of Lag0016368 vs. NCBI nr
Match: XP_023547495.1 (transcription factor bHLH91-like [Cucurbita pepo subsp. pepo] >XP_023547496.1 transcription factor bHLH91-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.9 bits (2132), Expect = 2.0e-235
Identity = 448/523 (85.66%), Postives = 470/523 (89.87%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPP-VRDASENGFPPTMAHIATLPSF----PPQLIAGGAGQEA 70
           MYEETACSDPNSMSAE TPP V DAS+NGFP TMAHI +LPSF    PP LI GG GQ+ 
Sbjct: 1   MYEETACSDPNSMSAEPTPPVVPDASQNGFPQTMAHIPSLPSFPPPPPPPLIVGG-GQDT 60

Query: 71  HANFDE-NLNLSMEELSY--HNNHRGGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFG 130
           HANFD+ NLNLSM+E+SY  H++HR G GDAMELEFQQQ         GFDN N+NP+FG
Sbjct: 61  HANFDDNNLNLSMDEISYHHHHHHRSGGGDAMELEFQQQG-------VGFDNCNMNPSFG 120

Query: 131 QEVTSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQD-QFSDSAMAAAYTQAPDLLNF 190
           QEV+SDSNRMVC DQS+WVG+QIQEMGFN  HH IQSQD QFSDSAMAAAYTQAPDLLNF
Sbjct: 121 QEVSSDSNRMVCLDQSAWVGSQIQEMGFN--HHLIQSQDHQFSDSAMAAAYTQAPDLLNF 180

Query: 191 FNMPAARCS-NSSISFS-NTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHL 250
           FNMPAARCS NSSISFS NTISNLHSSAMGGFLADLPAGD G NPSSTSLSILYDPLFHL
Sbjct: 181 FNMPAARCSNNSSISFSNNTISNLHSSAMGGFLADLPAGD-GSNPSSTSLSILYDPLFHL 240

Query: 251 NLPPQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE-MEREGTAGVYEDGDGSGVLE 310
           NLPPQPPLFRELFHSLPHGY LP+ASSRGRGGSLFPEGE MEREGTAGVYEDGDGSGVLE
Sbjct: 241 NLPPQPPLFRELFHSLPHGYGLPTASSRGRGGSLFPEGEIMEREGTAGVYEDGDGSGVLE 300

Query: 311 FSRDMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEY 370
           FSRDMADCIGKGRDGKMTKH   ERQRRVQLN+KYKALRSLVP+PTKTDRASIVGDAI Y
Sbjct: 301 FSRDMADCIGKGRDGKMTKHFATERQRRVQLNDKYKALRSLVPSPTKTDRASIVGDAINY 360

Query: 371 IKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYN 430
           IKELLR VKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAK   VGD  EDQSYN
Sbjct: 361 IKELLREVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKTGAVGDGGEDQSYN 420

Query: 431 LRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGD 490
           LRSSWLQRKTKDTEVDVRI+DDEVTIKLVQRK+NCLLLVSKLLDDLQLDLHHVAGGHIGD
Sbjct: 421 LRSSWLQRKTKDTEVDVRIVDDEVTIKLVQRKLNCLLLVSKLLDDLQLDLHHVAGGHIGD 480

Query: 491 YYSFLFNTKIYEGSSVYASAIANRVIEAVDRQYN--NNISHYS 520
           YYSFLFNTKIYEGSSVYASAIAN+V+EAVDRQYN  NN ++ S
Sbjct: 481 YYSFLFNTKIYEGSSVYASAIANKVMEAVDRQYNSTNNTANTS 512

BLAST of Lag0016368 vs. NCBI nr
Match: KAG6574832.1 (Transcription factor basic helix-loop-helix 91, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 807.4 bits (2084), Expect = 7.4e-230
Identity = 434/499 (86.97%), Postives = 452/499 (90.58%), Query Frame = 0

Query: 23  MSAEATPP-VRDASENGFPPTMAHIATLPSF--PPQLIAGGAGQEAHANFDE-NLNLSME 82
           MSAE TPP V DAS+NGFP TMAHI  LPSF  PP LI  G GQ+ HANFD+ NLNLSME
Sbjct: 1   MSAEPTPPVVPDASQNGFPQTMAHIPALPSFPPPPPLIVAG-GQDTHANFDDNNLNLSME 60

Query: 83  ELSYHNNHRGGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEVTSDSNRMVCFDQS 142
           E+SYH++HR G GDAMELEFQQQ         GFDN N+NP+FGQEV+SDSNRMVC DQS
Sbjct: 61  EISYHHHHRSGGGDAMELEFQQQG-------VGFDNCNMNPSFGQEVSSDSNRMVCLDQS 120

Query: 143 SWVGTQIQEMGFNHHHHQIQSQD-QFSDSAMAAAYTQAPDLLNFFNMPAARCS-NSSISF 202
           +WVG+QIQEMGFN  HH IQSQD QFSDSAMAAAYTQAPDLLNFFNMPAARCS NSSISF
Sbjct: 121 AWVGSQIQEMGFN--HHLIQSQDHQFSDSAMAAAYTQAPDLLNFFNMPAARCSNNSSISF 180

Query: 203 S-NTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQPPLFRELFHSL 262
           S NTISNLHSSAMGGFLADLPAGD G NPSSTSLSILYDPLFHLNLPPQPPLFRELFHSL
Sbjct: 181 SNNTISNLHSSAMGGFLADLPAGD-GSNPSSTSLSILYDPLFHLNLPPQPPLFRELFHSL 240

Query: 263 PHGYSLPSASSRGRGGSLFPEGE-MEREGTAGVYEDGDGSGVLEFSRDMADCIGKGRDGK 322
           PHGY LP+ASSRGRGGSLFPEGE MEREGTAGVYEDGDGSGVLEFSRDMADCIGKGRDGK
Sbjct: 241 PHGYGLPAASSRGRGGSLFPEGEIMEREGTAGVYEDGDGSGVLEFSRDMADCIGKGRDGK 300

Query: 323 MTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELLRTVKELKLLVE 382
           MTKH   ERQRRVQLN+KYKALRSLVP+PTKTDRASIVGDAI YIKELLR VKELKLLVE
Sbjct: 301 MTKHFATERQRRVQLNDKYKALRSLVPSPTKTDRASIVGDAINYIKELLREVKELKLLVE 360

Query: 383 KKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYNLRSSWLQRKTKDTEVD 442
           KKRCSRERSKRHRTAEEDGGAWDVESSNAK   VGD  EDQSYNLRSSWLQRKTKDTEVD
Sbjct: 361 KKRCSRERSKRHRTAEEDGGAWDVESSNAKTGAVGDGGEDQSYNLRSSWLQRKTKDTEVD 420

Query: 443 VRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSV 502
           VRI+DDEVTIKLVQRK+NCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSV
Sbjct: 421 VRIVDDEVTIKLVQRKLNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSV 480

Query: 503 YASAIANRVIEAVDRQYNN 514
           YASAIAN+V+EAVDRQYNN
Sbjct: 481 YASAIANKVMEAVDRQYNN 488

BLAST of Lag0016368 vs. ExPASy Swiss-Prot
Match: Q84TK1 (Transcription factor bHLH10 OS=Arabidopsis thaliana OX=3702 GN=BHLH10 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.5e-65
Identity = 198/521 (38.00%), Postives = 267/521 (51.25%), Query Frame = 0

Query: 5   EPSYRKMYEETACSDPNSMSAEATPPVRDASENGFPPTMAHIATLPSFPPQLIAGGAGQE 64
           E     +YEE  C DPN+       P     E+ F       A  P  PPQ++  G    
Sbjct: 2   EEERESLYEEMGCFDPNT-------PAEVTVESSFSQ-----AEPPPPPPQVLVAG---- 61

Query: 65  AHANFDENLNLSMEELSYHNNHRGGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQE 124
             +  + N ++ +EELS             E     Q    A++     + N  P     
Sbjct: 62  --STSNSNCSVEVEELS-------------EFHLSPQDCPQASSTPLQFHINPPPPPPPP 121

Query: 125 VTSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQDQFSDSAMAAAYTQAPDLLNFFNM 184
                N +            I +M  +   H            +       PDLL+  ++
Sbjct: 122 CDQLHNNL------------IHQMASHQQQHSNWDNGYQDFVNLGPNSATTPDLLSLLHL 181

Query: 185 PAARCSNSSISFSNTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQ 244
           P  RCS           N H S+M      LP        SS++ +++YDPLFHLN P Q
Sbjct: 182 P--RCS--------LPPNHHPSSM------LPTSFSDIMSSSSAAAVMYDPLFHLNFPMQ 241

Query: 245 PPLFRELFHSLPHGYSLPSASSR----GRGGS--LFPEGEMEREGTAGVYEDGDGSGVLE 304
           P   R+  + L +G  L     +      GG   L+ EG     G       G  + +LE
Sbjct: 242 P---RDQ-NQLRNGSCLLGVEDQIQMDANGGMNVLYFEGANNNNG-------GFENEILE 301

Query: 305 FSRDMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEY 364
           F+  +     KGR  + ++ SP ER+RRV  N+++  L++L+PNPTK DRASIVG+AI+Y
Sbjct: 302 FNNGVT---RKGRGSRKSRTSPTERERRVHFNDRFFDLKNLIPNPTKIDRASIVGEAIDY 361

Query: 365 IKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESS----NAKPAVVGDAA-- 424
           IKELLRT++E K+LVEKKRC R RSK+     E GG  D E      N KP    D +  
Sbjct: 362 IKELLRTIEEFKMLVEKKRCGRFRSKKRARVGEGGGGEDQEEEEDTVNYKPQSEVDQSCF 421

Query: 425 -EDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQ-RKVNCLLLVSKLLDDLQLDLHH 484
            ++ + +LR SWL+RK+K TEVDVRIIDDEVTIKLVQ +K+NCLL  +K+LD LQLDLHH
Sbjct: 422 NKNNNNSLRCSWLKRKSKVTEVDVRIIDDEVTIKLVQKKKINCLLFTTKVLDQLQLDLHH 449

Query: 485 VAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQY 512
           VAGG IG++YSFLFNTKI EGS VYAS IA+ ++E V++QY
Sbjct: 482 VAGGQIGEHYSFLFNTKICEGSCVYASGIADTLMEVVEKQY 449

BLAST of Lag0016368 vs. ExPASy Swiss-Prot
Match: Q8GX46 (Transcription factor bHLH91 OS=Arabidopsis thaliana OX=3702 GN=BHLH91 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.2e-60
Identity = 169/384 (44.01%), Postives = 220/384 (57.29%), Query Frame = 0

Query: 143 TQIQEMGFNHHHHQIQSQDQFSDSAMAAAYTQAPDLLNFFNMPAARCSNSSISFSNTISN 202
           ++ Q    ++++H I    Q S+      +  + D  NFF M  +  + ++    + +S 
Sbjct: 68  SETQNQNISNNNHLINQMIQESN------WGVSEDNSNFF-MNTSHPNTTTTPIPDLLSL 127

Query: 203 LHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQPPLFRELFHSLPHGYSLP 262
           LH          LP+ D       T     YDPLFHLNLPPQPPL     +S   GY L 
Sbjct: 128 LHLPRCS---MSLPSSDIMAGSCFT-----YDPLFHLNLPPQPPLIPSNDYS---GYLL- 187

Query: 263 SASSRGRGGSLFPEGEMEREGTAGVYEDGDGSGVLEFSRDMADCIGKGRDGKMTKHSPIE 322
                G   +   + +    G        D SG++EFS+++     KGR  +  K    E
Sbjct: 188 -----GIDTNTTTQRDESNVGDENNNAQFD-SGIIEFSKEIR---RKGRGKRKNKPFTTE 247

Query: 323 RQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRER 382
           R+RR  LNE+Y+AL+ L+P+P+K DRASI+ D I+YI EL R V ELK LVE+KRC    
Sbjct: 248 RERRCHLNERYEALKLLIPSPSKGDRASILQDGIDYINELRRRVSELKYLVERKRC---- 307

Query: 383 SKRHRTAEEDGG------------AWDVESSNAKPAVVGDAAEDQSYN--LRSSWLQRKT 442
             RH+  E D                D +  N +     D  +  S N  LR SWLQRK+
Sbjct: 308 GGRHKNNEVDDNNNNKNLDDHGNEDDDDDDENMEKKPESDVIDQCSSNNSLRCSWLQRKS 367

Query: 443 KDTEVDVRIIDDEVTIKLVQ-RKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTK 502
           K TEVDVRI+DDEVTIK+VQ +K+NCLLLVSK+LD LQLDLHHVAGG IG++YSFLFNTK
Sbjct: 368 KVTEVDVRIVDDEVTIKVVQKKKINCLLLVSKVLDQLQLDLHHVAGGQIGEHYSFLFNTK 419

Query: 503 IYEGSSVYASAIANRVIEAVDRQY 512
           IYEGS++YASAIANRVIE VD+ Y
Sbjct: 428 IYEGSTIYASAIANRVIEVVDKHY 419

BLAST of Lag0016368 vs. ExPASy Swiss-Prot
Match: Q9LND0 (Transcription factor bHLH89 OS=Arabidopsis thaliana OX=3702 GN=BHLH89 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.5e-55
Identity = 148/308 (48.05%), Postives = 189/308 (61.36%), Query Frame = 0

Query: 226 STSLSI-LYDPLFHLNLPPQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGEMEREGT 285
           +TS S+  YDPLFHLN P QPP                        GS     + + E  
Sbjct: 151 TTSSSVAAYDPLFHLNFPLQPP-----------------------NGSFMGVDQDQTETN 210

Query: 286 AGVYEDGDGSGVLEFSRDMADCIG-KGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNP 345
            GV    D     E + ++ D +  KGR  K  K  P ER+RRV   +++  L++L+PNP
Sbjct: 211 QGVNLMYD-----EENNNLDDGLNRKGRGSKKRKIFPTERERRVHFKDRFGDLKNLIPNP 270

Query: 346 TKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDV--ESS 405
           TK DRASIVG+AI+YIKELLRT+ E KLLVEKKR       + R  E D    DV  E+ 
Sbjct: 271 TKNDRASIVGEAIDYIKELLRTIDEFKLLVEKKRV------KQRNREGD----DVVDENF 330

Query: 406 NAKPAVVGDAAEDQSYN-LRSSWLQRKTKDTEVDVRIIDDEVTIKLVQ-RKVNCLLLVSK 465
            A+  VV     ++  N LR SWL+RK+K T+VDVRIIDDEVTIK+VQ +K+NCLL VSK
Sbjct: 331 KAQSEVVEQCLINKKNNALRCSWLKRKSKFTDVDVRIIDDEVTIKIVQKKKINCLLFVSK 390

Query: 466 LLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQY------NN 522
           ++D L+LDLHHVAG  IG+++SFLFN KI EGSSVYASAIA+RV+E + +QY      NN
Sbjct: 391 VVDQLELDLHHVAGAQIGEHHSFLFNAKISEGSSVYASAIADRVMEVLKKQYMEALSANN 420

BLAST of Lag0016368 vs. ExPASy Swiss-Prot
Match: Q7X8R0 (Transcription factor EAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=EAT1 PE=1 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 1.7e-51
Identity = 159/370 (42.97%), Postives = 211/370 (57.03%), Query Frame = 0

Query: 157 IQSQDQFSDSAM--AAAYTQAPDLLNFF--NMPAARCSNSSISFSNTISNLHSSAMGGFL 216
           ++ Q Q  DSA+     YT APDLLN     M  A  + +S+    T++  +   + G L
Sbjct: 113 VEQQIQQYDSALYPNGVYTPAPDLLNLMQCTMAPAFPATTSVFGDTTLNGTNYLDLNGEL 172

Query: 217 ADLPAGDGGGNPSSTSLSILYDPLFHLNL-PPQPPLFRELFHSLPHGYSL-PSASSR--- 276
             + A    G    + L    D    L     Q  L +++ HSLP  Y L PS   R   
Sbjct: 173 TGVAAVPDSG----SGLMFASDSALQLGYHGTQSHLIKDICHSLPQNYGLFPSEDERDVI 232

Query: 277 -GRG-GSLFPEGEMEREGTAGVYEDGDGSGVLEFSRDMADCIGKGR--DGKMTKHSPIER 336
            G G G LF E            +D     VLE  R      GKG    GK   +   ER
Sbjct: 233 IGVGSGDLFQE-----------IDDRQFDSVLECRR------GKGEFGKGKGKANFATER 292

Query: 337 QRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRERS 396
           +RR QLN K++ LR L PNPTK DRASIVGDAIEYI EL RTVKELK+LVE+KR    R 
Sbjct: 293 ERREQLNVKFRTLRMLFPNPTKNDRASIVGDAIEYIDELNRTVKELKILVEQKRHGNNRR 352

Query: 397 KRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYN-LRSSWLQRKTKDTEVDVRIIDDEV 456
           K  +  +E   A D ESS+ +P  V D  ++Q +  +RSSW+QR++K+  VDVRI+DDEV
Sbjct: 353 KVLKLDQE--AAADGESSSMRP--VRDDQDNQLHGAIRSSWVQRRSKECHVDVRIVDDEV 412

Query: 457 TIKLVQ-RKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIAN 512
            IKL + +K N LL  +K+LD+ QL+L HV GG IGD++ F+FNTK+ EGS+VYA A+A 
Sbjct: 413 NIKLTEKKKANSLLHAAKVLDEFQLELIHVVGGIIGDHHIFMFNTKVSEGSAVYACAVAK 457

BLAST of Lag0016368 vs. ExPASy Swiss-Prot
Match: Q5JNS0 (Transcription factor TIP2 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP2 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.8e-38
Identity = 97/196 (49.49%), Postives = 134/196 (68.37%), Query Frame = 0

Query: 322 ERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRE 381
           E+QRR++L EKY AL  L+PN TK DRA+++ DAIEYI+EL RTV+EL LLVEKKR  RE
Sbjct: 182 EKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEYIQELGRTVEELTLLVEKKRRRRE 241

Query: 382 -------RSKRHRTAEEDGGAWDVESSNAKPAVVGDAA-EDQSYNLRSSWLQRKTKDTEV 441
                   +     A  D  A   E      A +G  A   +   +RS+++QR++K+T V
Sbjct: 242 MQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMGAVAPPPRQAPIRSTYIQRRSKETFV 301

Query: 442 DVRIIDDEVTIKLVQRKVN-CLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGS 501
           DVRI++D+V IKL +R+ + CL   S+ LDDL+LDL H++GG IGD + ++FNTKI+ GS
Sbjct: 302 DVRIVEDDVNIKLTKRRRDGCLAAASRALDDLRLDLVHLSGGKIGDCHIYMFNTKIHSGS 361

Query: 502 SVYASAIANRVIEAVD 509
            V+ASA+A+R+IE VD
Sbjct: 362 PVFASAVASRLIEVVD 377

BLAST of Lag0016368 vs. ExPASy TrEMBL
Match: A0A6J1L271 (transcription factor bHLH91-like OS=Cucurbita maxima OX=3661 GN=LOC111499172 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.3e-240
Identity = 450/510 (88.24%), Postives = 467/510 (91.57%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPP-VRDASENGFPPTMAHIATLPSFPPQLIAGGAGQEAHANF 70
           MYEETACSDPNSMSAE TPP VRDASENGFP TMAHI TLPSFPP LI GG GQ++HANF
Sbjct: 1   MYEETACSDPNSMSAEPTPPGVRDASENGFPQTMAHIPTLPSFPPPLIVGG-GQDSHANF 60

Query: 71  DE-NLNLSMEELSYHNNHR-GGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEVTS 130
           D+ NLNLSMEE+SYH++HR GG GDAMELEFQQQ         GFDN N+NP+FGQEV+S
Sbjct: 61  DDNNLNLSMEEISYHHHHRSGGGGDAMELEFQQQ-------AVGFDNCNMNPSFGQEVSS 120

Query: 131 DSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQD-QFSDSAMAAAYTQAPDLLNFFNMPA 190
           DSNRMVC DQS+W GTQIQEMGFN  HH IQSQD QFSDSAMAAAYTQAPDLLNFFNMPA
Sbjct: 121 DSNRMVCLDQSAWAGTQIQEMGFN--HHLIQSQDHQFSDSAMAAAYTQAPDLLNFFNMPA 180

Query: 191 ARCS-NSSISFS-NTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQ 250
           ARCS NSSISFS NTISNLHSSAMGGFLADLPAGD G NPSSTSLSILYDPLFHLNLPPQ
Sbjct: 181 ARCSNNSSISFSNNTISNLHSSAMGGFLADLPAGD-GSNPSSTSLSILYDPLFHLNLPPQ 240

Query: 251 PPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE-MEREGTAGVYEDGDGSGVLEFSRDM 310
           PPLFRELFHSLPHGY LP+ASSRGRGGSLFPEGE MEREGTAGVYEDGDGSGVLEFSRDM
Sbjct: 241 PPLFRELFHSLPHGYGLPAASSRGRGGSLFPEGEIMEREGTAGVYEDGDGSGVLEFSRDM 300

Query: 311 ADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELL 370
           ADCIGKGRDGKMTKH   ERQRRVQLN+KYKALRSLVP+PTKTDRASIVGDAI YIKELL
Sbjct: 301 ADCIGKGRDGKMTKHFATERQRRVQLNDKYKALRSLVPSPTKTDRASIVGDAINYIKELL 360

Query: 371 RTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYNLRSSW 430
           R VKELKLLVEKKRCSRERSKRHRTAEEDG AWDVESSNAK   VGD  EDQSYNLRSSW
Sbjct: 361 REVKELKLLVEKKRCSRERSKRHRTAEEDGSAWDVESSNAKTVAVGDGGEDQSYNLRSSW 420

Query: 431 LQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFL 490
           LQRKTKDTEVDVRI+DDEVTIKLVQRK+NCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFL
Sbjct: 421 LQRKTKDTEVDVRIVDDEVTIKLVQRKLNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFL 480

Query: 491 FNTKIYEGSSVYASAIANRVIEAVDRQYNN 514
           FNTKIYEGSSVYASAIAN+V+EAVDRQYNN
Sbjct: 481 FNTKIYEGSSVYASAIANKVMEAVDRQYNN 499

BLAST of Lag0016368 vs. ExPASy TrEMBL
Match: A0A6J1H2Y8 (transcription factor bHLH91-like OS=Cucurbita moschata OX=3662 GN=LOC111459937 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 6.7e-237
Identity = 447/512 (87.30%), Postives = 465/512 (90.82%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPP-VRDASENGFPPTMAHIATLPSF--PPQLIAGGAGQEAHA 70
           MYEETACSDPNSMSAE TPP V DAS+NGFP TMAHI  LPSF  PP LI  G GQ+ HA
Sbjct: 1   MYEETACSDPNSMSAEPTPPVVPDASQNGFPQTMAHIPALPSFPPPPPLIVAG-GQDTHA 60

Query: 71  NFDE-NLNLSMEELSYHNNHR-GGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEV 130
           NFD+ NLNLSMEE+SYH++HR GG GDAMELEFQQQ         GFDN N+NP+FGQEV
Sbjct: 61  NFDDNNLNLSMEEISYHHHHRSGGGGDAMELEFQQQG-------VGFDNCNMNPSFGQEV 120

Query: 131 TSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQD-QFSDSAMAAAYTQAPDLLNFFNM 190
           +SDSNRMVC DQS+WVG+QIQEMGFN  HH IQSQD QFSDSAMAAAYTQAPDLLNFFNM
Sbjct: 121 SSDSNRMVCLDQSAWVGSQIQEMGFN--HHLIQSQDHQFSDSAMAAAYTQAPDLLNFFNM 180

Query: 191 PAARCS-NSSISFS-NTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLP 250
           PAARCS NSSISFS NTISNLHSSAMGGFLADLPAGD G NPSSTSLSILYDPLFHLNLP
Sbjct: 181 PAARCSNNSSISFSNNTISNLHSSAMGGFLADLPAGD-GSNPSSTSLSILYDPLFHLNLP 240

Query: 251 PQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE-MEREGTAGVYEDGDGSGVLEFSR 310
           PQPPLFRELFHSLPHGY LP+ASSRGRGGSLFPEGE MEREGTAGVYEDGDGSGVLEFSR
Sbjct: 241 PQPPLFRELFHSLPHGYGLPAASSRGRGGSLFPEGEIMEREGTAGVYEDGDGSGVLEFSR 300

Query: 311 DMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKE 370
           DMADCIGKGRDGKMTKH   ERQRRVQLN+KYKALRSLVP+PTKTDRASIVGDAI YIKE
Sbjct: 301 DMADCIGKGRDGKMTKHFATERQRRVQLNDKYKALRSLVPSPTKTDRASIVGDAINYIKE 360

Query: 371 LLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYNLRS 430
           LLR VKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAK   VGD  EDQSYNLRS
Sbjct: 361 LLREVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKTGAVGDGGEDQSYNLRS 420

Query: 431 SWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYS 490
           SWLQRKTKDTEVDVRI+DDEVTIKLVQRK+NCLLLVSKLLDDLQLDLHHVAGGHIGDYYS
Sbjct: 421 SWLQRKTKDTEVDVRIVDDEVTIKLVQRKLNCLLLVSKLLDDLQLDLHHVAGGHIGDYYS 480

Query: 491 FLFNTKIYEGSSVYASAIANRVIEAVDRQYNN 514
           FLFNTKIYEGSSVYASAIAN+V+EAVDRQYNN
Sbjct: 481 FLFNTKIYEGSSVYASAIANKVMEAVDRQYNN 501

BLAST of Lag0016368 vs. ExPASy TrEMBL
Match: A0A6J1DNV7 (transcription factor bHLH91-like OS=Momordica charantia OX=3673 GN=LOC111022828 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 1.0e-221
Identity = 430/520 (82.69%), Postives = 448/520 (86.15%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPPVRDASENGFPPTMAHIATLPSFPPQLIAGGAGQEAHANFD 70
           MYEET CSDPNSMSAEA   VR+ SENGFP TM HIA LPSFPP LIAGG+ QE  A F+
Sbjct: 1   MYEETGCSDPNSMSAEA---VREVSENGFPQTMVHIAALPSFPPPLIAGGS-QENDAGFE 60

Query: 71  ENLNLSMEELSYHNNHR-GGSGDAMELEFQQQQAAAAAAVAGFDNGN-------VNPNFG 130
           ENLNLSMEELSYH  HR GG GDAMELEFQQQ AAA     GFDN         +NPNF 
Sbjct: 61  ENLNLSMEELSYH--HRGGGGGDAMELEFQQQSAAA----VGFDNSTHDGGINIINPNFA 120

Query: 131 QEV-TSDSNRMVCFDQSSWVGTQIQEM--GFNHHHHQIQSQD--QFSDSAMAAAYTQAPD 190
           Q+  T DSNRMVCFDQS+WVG QIQEM  GFNHHHHQIQSQ+  QF DSAMAA  TQAPD
Sbjct: 121 QDQGTGDSNRMVCFDQSNWVGNQIQEMGLGFNHHHHQIQSQEQQQFPDSAMAAYSTQAPD 180

Query: 191 LLNFFNMPAARCSNSSISFSN---TISNLHSSAMGGFLADLPAGD-GGGNPSSTSLSILY 250
           LLNFFN+PAARCSNSS    N   + SNLHSSAMGGFL DLPAG    GNPSSTSLSILY
Sbjct: 181 LLNFFNLPAARCSNSSPVLPNSSISFSNLHSSAMGGFLTDLPAGGVDSGNPSSTSLSILY 240

Query: 251 DPLFHLNLPPQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGEMEREGTAGVYEDGDG 310
           DPLFHLNLPPQPPLFRELFHSLPHGY LP+ASSRGRGGSLFPEGEMEREGTAGVYEDGDG
Sbjct: 241 DPLFHLNLPPQPPLFRELFHSLPHGYGLPAASSRGRGGSLFPEGEMEREGTAGVYEDGDG 300

Query: 311 SGVLEFSRDMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVG 370
           SGVLEF+RDMADC+GKGRDGKMTKH   ERQRRVQLN+KYKALRSLVPNPTKTDRASIVG
Sbjct: 301 SGVLEFTRDMADCMGKGRDGKMTKHFATERQRRVQLNDKYKALRSLVPNPTKTDRASIVG 360

Query: 371 DAIEYIKELLRTVKELKLLVEKKRCSRERSKRHRTA--EEDGGAWDVESSNAKPAVVGDA 430
           DAIEYIKELLRTVKELKLLVEKKRCSRERSKRHRT   EE GGAWDVESSN K AV  +A
Sbjct: 361 DAIEYIKELLRTVKELKLLVEKKRCSRERSKRHRTTAEEEGGGAWDVESSNGK-AVAAEA 420

Query: 431 --AEDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQLDLH 490
             AEDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRK+NCLL+VSKLLDDL LDLH
Sbjct: 421 AGAEDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRKLNCLLIVSKLLDDLHLDLH 480

Query: 491 HVAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDR 510
           HVAGGHIGDYYSFLFNTKIYEGSSVYASAIA++VIEAVDR
Sbjct: 481 HVAGGHIGDYYSFLFNTKIYEGSSVYASAIASKVIEAVDR 509

BLAST of Lag0016368 vs. ExPASy TrEMBL
Match: A0A0A0LI11 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G120400 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 9.5e-183
Identity = 378/527 (71.73%), Postives = 405/527 (76.85%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPPVRDASENGFPPTMAHIATLP-SFPPQLIAGGAGQEAHANF 70
           MYEET CSDPNS+S               P TM HI+  P SFPP LIA    Q+ H NF
Sbjct: 1   MYEETECSDPNSIS---------------PETMPHISAFPNSFPPPLIA----QQTHPNF 60

Query: 71  --DENLNLSMEELSYHNNHRG-GSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEVT 130
             + NLNLS++ +SYH++       DAMEL+FQQ   AAAA   GFD         QE+T
Sbjct: 61  HHNNNLNLSIDHISYHHHSTALQPADAMELDFQQ---AAAAPTPGFD---------QELT 120

Query: 131 SDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQDQFSDSAM-AAAYTQAPDLLNFFNM- 190
           SDSN M+C DQS+WVGTQIQEMGFNH+H     Q QFSDSA+    YTQ PDLLNF NM 
Sbjct: 121 SDSNPMLCLDQSNWVGTQIQEMGFNHNH----VQSQFSDSAIPPTPYTQPPDLLNFLNMP 180

Query: 191 PAARCS-NSSISFSNTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPP 250
           P ARCS NSSISF    SNLH+ AMG FL DLP GD   N SSTSLSILYDPLFHLNLPP
Sbjct: 181 PTARCSNNSSISF----SNLHTPAMGAFLGDLPPGD-APNSSSTSLSILYDPLFHLNLPP 240

Query: 251 QPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE--MEREGTAGVYEDGDGSGVLEFSR 310
           QPPLFRELFHSLPHGY +P+ASSRGRGGSLFPEG   +EREGTAGVYEDGDGSGVLEFSR
Sbjct: 241 QPPLFRELFHSLPHGYGMPAASSRGRGGSLFPEGSEIVEREGTAGVYEDGDGSGVLEFSR 300

Query: 311 DMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKE 370
           DMADCIGK RDGKMTKH   ERQRRVQLN+KYKALRSLVP PTK DRASIVGDAI YI+E
Sbjct: 301 DMADCIGKRRDGKMTKHFATERQRRVQLNDKYKALRSLVPIPTKNDRASIVGDAINYIQE 360

Query: 371 LLRTVKELKLLVEKKRCSRERSKRHRTAEE---------------DGGAWDVESSNAKPA 430
           LLR VKELKLLVEKKR SRERSKR RTAEE                GGAWDVESSNAK  
Sbjct: 361 LLREVKELKLLVEKKRSSRERSKRVRTAEEIEQGGGSGGGGGGGGGGGAWDVESSNAKGG 420

Query: 431 VVGDAAEDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDLQL 490
                 EDQ YNLRSSWLQRKTKDTEVDVRI+DDEVT+KLVQRK+NCLLLVSKLL+DLQL
Sbjct: 421 --EGVVEDQRYNLRSSWLQRKTKDTEVDVRIVDDEVTVKLVQRKLNCLLLVSKLLEDLQL 480

Query: 491 DLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQYNN 514
           DLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIAN+V+EAVDRQYNN
Sbjct: 481 DLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANKVMEAVDRQYNN 485

BLAST of Lag0016368 vs. ExPASy TrEMBL
Match: A0A5D3BUB3 (Transcription factor bHLH91-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold775G00110 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 2.1e-182
Identity = 376/529 (71.08%), Postives = 405/529 (76.56%), Query Frame = 0

Query: 11  MYEETACSDPNSMSAEATPPVRDASENGFPPTMAHIATL--PSFPPQLIAGGAGQEAHAN 70
           MYEET CSDPNS+S               P TM HI+    PSFPP LIA    Q+ H N
Sbjct: 1   MYEETECSDPNSIS---------------PETMPHISAFPNPSFPPPLIA----QQTHPN 60

Query: 71  F---DENLNLSMEELSYHNNHRGGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQEV 130
           F   + NLNLS++ +SYH++      DAMEL+FQ    AAAA   GFD         +++
Sbjct: 61  FHHNNNNLNLSIDHISYHHHSTTLQPDAMELDFQH---AAAAPTPGFDQ--------EQL 120

Query: 131 TSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQDQFSDSAM-AAAYTQAPDLLNFFNM 190
           TSDSN M+C DQS+WVGTQIQEMGFNH+H     Q QFSDSA+    YTQ PDLLNF NM
Sbjct: 121 TSDSNPMLCLDQSNWVGTQIQEMGFNHNH----IQSQFSDSAIPPTPYTQPPDLLNFLNM 180

Query: 191 -PAARCS-NSSISFSNTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLP 250
            P ARCS NSSISF    SNLH+SAMG FL DLP GD   N SSTSLSILYDPLFHLNLP
Sbjct: 181 PPTARCSNNSSISF----SNLHTSAMGAFLGDLPPGD-TPNSSSTSLSILYDPLFHLNLP 240

Query: 251 PQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGE--MEREGTAGVYEDGDGSGVLEFS 310
           PQPPLFRELFHSLPHGY +P+ASSRGRGGSLFPEG   +EREGTAGVYEDGDGSGVLEFS
Sbjct: 241 PQPPLFRELFHSLPHGYGMPAASSRGRGGSLFPEGSEIVEREGTAGVYEDGDGSGVLEFS 300

Query: 311 RDMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIK 370
           RDMADCIGK RDGKMTKH   ERQRRVQLN+KYKALRSLVP PTK DRASIVGDAI YI+
Sbjct: 301 RDMADCIGKRRDGKMTKHFATERQRRVQLNDKYKALRSLVPIPTKNDRASIVGDAINYIQ 360

Query: 371 ELLRTVKELKLLVEKKRCSRERSKRHRTAEE----------------DGGAWDVESSNAK 430
           ELLR VKELKLLVEKKR SRERSKR RTAEE                 GGAWDVESSNAK
Sbjct: 361 ELLREVKELKLLVEKKRSSRERSKRVRTAEEIEQGGGGGGGGGGGGSGGGAWDVESSNAK 420

Query: 431 PAVVGDAAEDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQRKVNCLLLVSKLLDDL 490
                   EDQ YNLRSSWLQRKTKDTEVDVRI+DDEVT+KLVQRK+NCLLLVSKLL+DL
Sbjct: 421 GG--EGVVEDQRYNLRSSWLQRKTKDTEVDVRIVDDEVTVKLVQRKLNCLLLVSKLLEDL 480

Query: 491 QLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQYNN 514
           QLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIAN+V+EAVDRQYNN
Sbjct: 481 QLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANKVMEAVDRQYNN 488

BLAST of Lag0016368 vs. TAIR 10
Match: AT2G31220.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 250.8 bits (639), Expect = 2.5e-66
Identity = 198/521 (38.00%), Postives = 267/521 (51.25%), Query Frame = 0

Query: 5   EPSYRKMYEETACSDPNSMSAEATPPVRDASENGFPPTMAHIATLPSFPPQLIAGGAGQE 64
           E     +YEE  C DPN+       P     E+ F       A  P  PPQ++  G    
Sbjct: 2   EEERESLYEEMGCFDPNT-------PAEVTVESSFSQ-----AEPPPPPPQVLVAG---- 61

Query: 65  AHANFDENLNLSMEELSYHNNHRGGSGDAMELEFQQQQAAAAAAVAGFDNGNVNPNFGQE 124
             +  + N ++ +EELS             E     Q    A++     + N  P     
Sbjct: 62  --STSNSNCSVEVEELS-------------EFHLSPQDCPQASSTPLQFHINPPPPPPPP 121

Query: 125 VTSDSNRMVCFDQSSWVGTQIQEMGFNHHHHQIQSQDQFSDSAMAAAYTQAPDLLNFFNM 184
                N +            I +M  +   H            +       PDLL+  ++
Sbjct: 122 CDQLHNNL------------IHQMASHQQQHSNWDNGYQDFVNLGPNSATTPDLLSLLHL 181

Query: 185 PAARCSNSSISFSNTISNLHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQ 244
           P  RCS           N H S+M      LP        SS++ +++YDPLFHLN P Q
Sbjct: 182 P--RCS--------LPPNHHPSSM------LPTSFSDIMSSSSAAAVMYDPLFHLNFPMQ 241

Query: 245 PPLFRELFHSLPHGYSLPSASSR----GRGGS--LFPEGEMEREGTAGVYEDGDGSGVLE 304
           P   R+  + L +G  L     +      GG   L+ EG     G       G  + +LE
Sbjct: 242 P---RDQ-NQLRNGSCLLGVEDQIQMDANGGMNVLYFEGANNNNG-------GFENEILE 301

Query: 305 FSRDMADCIGKGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEY 364
           F+  +     KGR  + ++ SP ER+RRV  N+++  L++L+PNPTK DRASIVG+AI+Y
Sbjct: 302 FNNGVT---RKGRGSRKSRTSPTERERRVHFNDRFFDLKNLIPNPTKIDRASIVGEAIDY 361

Query: 365 IKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDVESS----NAKPAVVGDAA-- 424
           IKELLRT++E K+LVEKKRC R RSK+     E GG  D E      N KP    D +  
Sbjct: 362 IKELLRTIEEFKMLVEKKRCGRFRSKKRARVGEGGGGEDQEEEEDTVNYKPQSEVDQSCF 421

Query: 425 -EDQSYNLRSSWLQRKTKDTEVDVRIIDDEVTIKLVQ-RKVNCLLLVSKLLDDLQLDLHH 484
            ++ + +LR SWL+RK+K TEVDVRIIDDEVTIKLVQ +K+NCLL  +K+LD LQLDLHH
Sbjct: 422 NKNNNNSLRCSWLKRKSKVTEVDVRIIDDEVTIKLVQKKKINCLLFTTKVLDQLQLDLHH 449

Query: 485 VAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQY 512
           VAGG IG++YSFLFNTKI EGS VYAS IA+ ++E V++QY
Sbjct: 482 VAGGQIGEHYSFLFNTKICEGSCVYASGIADTLMEVVEKQY 449

BLAST of Lag0016368 vs. TAIR 10
Match: AT2G31210.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 8.3e-62
Identity = 169/384 (44.01%), Postives = 220/384 (57.29%), Query Frame = 0

Query: 143 TQIQEMGFNHHHHQIQSQDQFSDSAMAAAYTQAPDLLNFFNMPAARCSNSSISFSNTISN 202
           ++ Q    ++++H I    Q S+      +  + D  NFF M  +  + ++    + +S 
Sbjct: 68  SETQNQNISNNNHLINQMIQESN------WGVSEDNSNFF-MNTSHPNTTTTPIPDLLSL 127

Query: 203 LHSSAMGGFLADLPAGDGGGNPSSTSLSILYDPLFHLNLPPQPPLFRELFHSLPHGYSLP 262
           LH          LP+ D       T     YDPLFHLNLPPQPPL     +S   GY L 
Sbjct: 128 LHLPRCS---MSLPSSDIMAGSCFT-----YDPLFHLNLPPQPPLIPSNDYS---GYLL- 187

Query: 263 SASSRGRGGSLFPEGEMEREGTAGVYEDGDGSGVLEFSRDMADCIGKGRDGKMTKHSPIE 322
                G   +   + +    G        D SG++EFS+++     KGR  +  K    E
Sbjct: 188 -----GIDTNTTTQRDESNVGDENNNAQFD-SGIIEFSKEIR---RKGRGKRKNKPFTTE 247

Query: 323 RQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRER 382
           R+RR  LNE+Y+AL+ L+P+P+K DRASI+ D I+YI EL R V ELK LVE+KRC    
Sbjct: 248 RERRCHLNERYEALKLLIPSPSKGDRASILQDGIDYINELRRRVSELKYLVERKRC---- 307

Query: 383 SKRHRTAEEDGG------------AWDVESSNAKPAVVGDAAEDQSYN--LRSSWLQRKT 442
             RH+  E D                D +  N +     D  +  S N  LR SWLQRK+
Sbjct: 308 GGRHKNNEVDDNNNNKNLDDHGNEDDDDDDENMEKKPESDVIDQCSSNNSLRCSWLQRKS 367

Query: 443 KDTEVDVRIIDDEVTIKLVQ-RKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNTK 502
           K TEVDVRI+DDEVTIK+VQ +K+NCLLLVSK+LD LQLDLHHVAGG IG++YSFLFNTK
Sbjct: 368 KVTEVDVRIVDDEVTIKVVQKKKINCLLLVSKVLDQLQLDLHHVAGGQIGEHYSFLFNTK 419

Query: 503 IYEGSSVYASAIANRVIEAVDRQY 512
           IYEGS++YASAIANRVIE VD+ Y
Sbjct: 428 IYEGSTIYASAIANRVIEVVDKHY 419

BLAST of Lag0016368 vs. TAIR 10
Match: AT1G06170.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.8e-56
Identity = 148/308 (48.05%), Postives = 189/308 (61.36%), Query Frame = 0

Query: 226 STSLSI-LYDPLFHLNLPPQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGEMEREGT 285
           +TS S+  YDPLFHLN P QPP                        GS     + + E  
Sbjct: 151 TTSSSVAAYDPLFHLNFPLQPP-----------------------NGSFMGVDQDQTETN 210

Query: 286 AGVYEDGDGSGVLEFSRDMADCIG-KGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNP 345
            GV    D     E + ++ D +  KGR  K  K  P ER+RRV   +++  L++L+PNP
Sbjct: 211 QGVNLMYD-----EENNNLDDGLNRKGRGSKKRKIFPTERERRVHFKDRFGDLKNLIPNP 270

Query: 346 TKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDV--ESS 405
           TK DRASIVG+AI+YIKELLRT+ E KLLVEKKR       + R  E D    DV  E+ 
Sbjct: 271 TKNDRASIVGEAIDYIKELLRTIDEFKLLVEKKRV------KQRNREGD----DVVDENF 330

Query: 406 NAKPAVVGDAAEDQSYN-LRSSWLQRKTKDTEVDVRIIDDEVTIKLVQ-RKVNCLLLVSK 465
            A+  VV     ++  N LR SWL+RK+K T+VDVRIIDDEVTIK+VQ +K+NCLL VSK
Sbjct: 331 KAQSEVVEQCLINKKNNALRCSWLKRKSKFTDVDVRIIDDEVTIKIVQKKKINCLLFVSK 390

Query: 466 LLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQY------NN 522
           ++D L+LDLHHVAG  IG+++SFLFN KI EGSSVYASAIA+RV+E + +QY      NN
Sbjct: 391 VVDQLELDLHHVAGAQIGEHHSFLFNAKISEGSSVYASAIADRVMEVLKKQYMEALSANN 420

BLAST of Lag0016368 vs. TAIR 10
Match: AT1G06170.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.8e-56
Identity = 148/308 (48.05%), Postives = 189/308 (61.36%), Query Frame = 0

Query: 226 STSLSI-LYDPLFHLNLPPQPPLFRELFHSLPHGYSLPSASSRGRGGSLFPEGEMEREGT 285
           +TS S+  YDPLFHLN P QPP                        GS     + + E  
Sbjct: 151 TTSSSVAAYDPLFHLNFPLQPP-----------------------NGSFMGVDQDQTETN 210

Query: 286 AGVYEDGDGSGVLEFSRDMADCIG-KGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNP 345
            GV    D     E + ++ D +  KGR  K  K  P ER+RRV   +++  L++L+PNP
Sbjct: 211 QGVNLMYD-----EENNNLDDGLNRKGRGSKKRKIFPTERERRVHFKDRFGDLKNLIPNP 270

Query: 346 TKTDRASIVGDAIEYIKELLRTVKELKLLVEKKRCSRERSKRHRTAEEDGGAWDV--ESS 405
           TK DRASIVG+AI+YIKELLRT+ E KLLVEKKR       + R  E D    DV  E+ 
Sbjct: 271 TKNDRASIVGEAIDYIKELLRTIDEFKLLVEKKRV------KQRNREGD----DVVDENF 330

Query: 406 NAKPAVVGDAAEDQSYN-LRSSWLQRKTKDTEVDVRIIDDEVTIKLVQ-RKVNCLLLVSK 465
            A+  VV     ++  N LR SWL+RK+K T+VDVRIIDDEVTIK+VQ +K+NCLL VSK
Sbjct: 331 KAQSEVVEQCLINKKNNALRCSWLKRKSKFTDVDVRIIDDEVTIKIVQKKKINCLLFVSK 390

Query: 466 LLDDLQLDLHHVAGGHIGDYYSFLFNTKIYEGSSVYASAIANRVIEAVDRQY------NN 522
           ++D L+LDLHHVAG  IG+++SFLFN KI EGSSVYASAIA+RV+E + +QY      NN
Sbjct: 391 VVDQLELDLHHVAGAQIGEHHSFLFNAKISEGSSVYASAIADRVMEVLKKQYMEALSANN 420

BLAST of Lag0016368 vs. TAIR 10
Match: AT2G16910.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 2.1e-09
Identity = 58/199 (29.15%), Postives = 94/199 (47.24%), Query Frame = 0

Query: 309 KGRDGKMTKHSPIERQRRVQLNEKYKALRSLVPNPTKTDRASIVGDAIEYIKELLRTVKE 368
           K   G   K+   ER+RR +LN++  ALRSLVP  TK DRASI+GDAI Y+KEL    KE
Sbjct: 306 KSGKGSQAKNLMAERRRRKKLNDRLYALRSLVPRITKLDRASILGDAINYVKELQNEAKE 365

Query: 369 LKLLVEKKRCSRERSKRHRTAEEDGGAWDVESSNAKPAVVGDAAEDQSYNLRSSWLQRKT 428
           L+  +E+   + + S R +      G                 +  Q  +L +S  + + 
Sbjct: 366 LQDELEENSETEDGSNRPQGGMSLNGTVVTGFHPGLSCNSNVPSVKQDVDLENSNDKGQE 425

Query: 429 KDTEVDVRIIDD-EVTIKLV-QRKVNCLLLVSKLLDDLQLDLHHVAGGHIGDYYSFLFNT 488
            + +VDV  +D  E  +K++ + K      + + LD L L++ +          S +F  
Sbjct: 426 MEPQVDVAQLDGREFFVKVICEYKPGGFTRLMEALDSLGLEVTNANTTRYLSLVSNVFKV 485

Query: 489 KIYEGSSVYASAIANRVIE 506
           +  +   V A  + N ++E
Sbjct: 486 EKNDNEMVQAEHVRNSLLE 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023006449.12.7e-24088.24transcription factor bHLH91-like [Cucurbita maxima] >XP_023006450.1 transcriptio... [more]
XP_022958755.11.4e-23687.30transcription factor bHLH91-like [Cucurbita moschata] >XP_022958756.1 transcript... [more]
KAG7013409.11.5e-23586.60Transcription factor bHLH91 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023547495.12.0e-23585.66transcription factor bHLH91-like [Cucurbita pepo subsp. pepo] >XP_023547496.1 tr... [more]
KAG6574832.17.4e-23086.97Transcription factor basic helix-loop-helix 91, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
Q84TK13.5e-6538.00Transcription factor bHLH10 OS=Arabidopsis thaliana OX=3702 GN=BHLH10 PE=1 SV=1[more]
Q8GX461.2e-6044.01Transcription factor bHLH91 OS=Arabidopsis thaliana OX=3702 GN=BHLH91 PE=2 SV=1[more]
Q9LND02.5e-5548.05Transcription factor bHLH89 OS=Arabidopsis thaliana OX=3702 GN=BHLH89 PE=1 SV=1[more]
Q7X8R01.7e-5142.97Transcription factor EAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=EAT1 PE=1 ... [more]
Q5JNS02.8e-3849.49Transcription factor TIP2 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1L2711.3e-24088.24transcription factor bHLH91-like OS=Cucurbita maxima OX=3661 GN=LOC111499172 PE=... [more]
A0A6J1H2Y86.7e-23787.30transcription factor bHLH91-like OS=Cucurbita moschata OX=3662 GN=LOC111459937 P... [more]
A0A6J1DNV71.0e-22182.69transcription factor bHLH91-like OS=Momordica charantia OX=3673 GN=LOC111022828 ... [more]
A0A0A0LI119.5e-18371.73BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G120400 PE=4 S... [more]
A0A5D3BUB32.1e-18271.08Transcription factor bHLH91-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT2G31220.12.5e-6638.00basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G31210.18.3e-6244.01basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G06170.11.8e-5648.05basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G06170.21.8e-5648.05basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G16910.12.1e-0929.15basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 319..368
e-value: 5.4E-13
score: 59.2
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 316..362
e-value: 1.4E-11
score: 44.2
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 313..362
score: 16.234097
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 310..376
e-value: 5.7E-14
score: 53.5
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 311..377
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR46834TRANSCRIPTION FACTOR BHLH91coord: 11..513
NoneNo IPR availablePANTHERPTHR46834:SF1TRANSCRIPTION FACTOR BHLH91coord: 11..513
NoneNo IPR availableCDDcd18918bHLH_AtMYC1_likecoord: 318..384
e-value: 1.08342E-33
score: 120.131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016368.1Lag0016368.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity