Lag0016103 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016103
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionrop guanine nucleotide exchange factor 3-like
Locationchr12: 33157605 .. 33160258 (-)
RNA-Seq ExpressionLag0016103
SyntenyLag0016103
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAACTTTCCATCTCTGAGGATAATTTTCGTCTGGGTTTTCGTCCTTCGTCGTCTTCCATGGATCTCAACGACCAATCCACCATTTCTGAAACTTCCGGCCACTCTCCGGCCAGCGGCTACTCTTTCTCTTACTGCAGAACCAGCTCCGACGCCTCCGCCTTCTCCGACCCCACCACCGACGACAGCAGTTTCTGCACTGAGCCCTCTCCGGCCATTCTCGCCAGACAGAGCACCAGAAAATTTAAACTCGCCGTCGGAGATAAGGTCGACGATCAGGACGCCATGGATTCCGGTGAGTGCGGTGTCGGAATTACAACTTTATCCTTCCACTTTTGTTAGTATAAATTCCAACGCTTTTGAGGTTTGTTGCAGAAGTAGAGATGATGAAGGAGAGATTTGCAAAGCTTTTGCTGGGAGAAGACATGTCAGGAAGTGGGAAAGGAGTTTGTACGGCGGTTACAATTTCAAACGCCATTACTAATCTTTACGGTCGGATTCTTCTTCTGAAAAATTCTTCAAACTGTGTTCAATGTTTTTTTTTGTTTCTGGTTTGGTTTTGAAGTTTTGTTTTGTTTGATCTCAGCGACGGTGTTCGGACAGAATCTGAAGCTGGAGCCGTTGCCGCCGGAGAAGAAAGCCATGTGGAAGAGAGAGATGACTTGTTTGTTGTCTGTTTGTGATTACATTGTTGAGTTCTTCACTGAATCTCAAACCTTGGAAGATGGAACAACTATGGAGGTGAACTACTTTTTATTTCCCCTTTTTTGCTTTTTTTTTTGGAACAAAAATGGTGGGTACAAGGATTGTCTGGTCATAAAATTCTCAAAACAGTTATTTCGACGGGTGCCCAAAATTTTAATGGGATGCATAGTGATGTGTAGGGATCGCAAAACGTGGACAACTAAGAGAGAGAAAATCTCACTTAGTTGTCGAGATTTAAGTGAGAAGGGAGATTCCTACATTAGTATGTACCCAAGTATTGTTCAACAAGCAGCCTTGGTTCTTTCGTACTTTTTTCTTTTTGGGTAATACTAATTCTCACAAAGTTATGAAATCATGATGGTGTTTTCAGGTGTTGAACAGCAGACAAAGGTCAGATATATACCTAAACCTTCCAGCATTGCAAAAGCTTGACATGATGCTCTTGGTAAAGACCATAAAAATCTTTCATGTTTCAAAATGGTAGCAAAAATGTTAACTTATTTGAGAGTGATCAATCTTGTTTGCCTCCGGCAGGACATTCTGGACAGTTTTCAGGACGCCGAGTTCTGGTACTCGGAAAAAGGAAGCATGTCTTCGGACTCGAATCGTTCGAGATCTGGTTCGTTTCGCCGACTCTCGTTGCAAAAAGAGGAAAAATGGTGGCTGCCAGTTCCCTGCATTCCCTCATGTGGTCTATCGGAGAAGGCGCGAAAACACCTTCGAAATAACCGCGAATGTGCGAACCAAATCCACAAAGCTGCCATGGCAATCAACAGCACTATTCTTGCCGAAATGGAAATTCCAGAGCCATACGTCGAAACTCTTCCAAAGGTTAGTCCCCTAATCGTAACATTTCTAGTATTATAGCTTTGTTTTCTAATATGTTTTCATGAAATGATCCTTTTTTTTTTGTTCAGAGTGGAAAGGCGAGTATAGGCGATATGATATACCGATACATGTATGGCGCAGACAAATTCTCACCCGATTATCTGCTCGATTGCTTAAACATAGGATCCGAACACGACGCGCTCGAACTTGCAGACCGAATCGAGGCTTCAATGTATACATGGAGAAGGAAAGCATGTCTAAGCCACTCAAAATCTTCATGGGAATTGGTGAAGGATCTAATGGCTGAAACAGACAAGGGAGACAAGAACACTGTTTTAGCAGAAAGAGCTGAGAGCTTGCTTTTGTCCTTGAAACAAAGGTTCCCTGAACTTTCTCAAACAACCTTAGACACTAGCAAGATTCAATACAATAGGGTAAGTTAAAAGGAAGCATTTTTTTTTTCTCTCTTAAATTACATGTTTAGTCGTTAAACTTTCAGAGTTATGTCTGATGATAAGTCTCTAAGTCCCTATTTGATAACTATTTGGTTTTTGAAAATTAGACTGGTTTTCTTTAAATTTCCTCACTATGGTTTTCATTTTTGTTAAAGACACACTTGAATTCCTAACTAAATTCTAAAAACAAGTTTTTGAAAATTATTTTTTTTTTGTTTTAAAAAATTGGTTTCGTTTTTGAAACATGAGAAGAAGGTAGGTGAAAAGCCAACGAAACTTGGAGTTGAGAGTGGTGTTTATAAGCTTAAAAACCAAAAACCAAATGGTTATCAAATGGGTCCAAACTATCAAAATTTTTAGTTGGTTTTTGCAAACTAGTGTTATCAAACCTAACAGACACAAAATTGAAAGTTTAGGGATCTATTAAACGCTTTTTCAAGTTCAGGGATTGACATTGTATTTAACTTTTTTTTTATATATTTATAAGTTTTAAAAATATAACATTTGCAAAAAGATGAATGGATTTTTTTTTTTATGTTGTAGGATGTGGGGCAAGCAGTGTTGGAGAGCTACTCAAGAGTGTTGGAAGGACTGGCATTCAACATTGTGGCTTGGATTGAAGATGTTCTTTTTGCTGATAGGTCCATAAGAAATCAAGAGTAG

mRNA sequence

ATGGAGAAACTTTCCATCTCTGAGGATAATTTTCGTCTGGGTTTTCGTCCTTCGTCGTCTTCCATGGATCTCAACGACCAATCCACCATTTCTGAAACTTCCGGCCACTCTCCGGCCAGCGGCTACTCTTTCTCTTACTGCAGAACCAGCTCCGACGCCTCCGCCTTCTCCGACCCCACCACCGACGACAGCAGTTTCTGCACTGAGCCCTCTCCGGCCATTCTCGCCAGACAGAGCACCAGAAAATTTAAACTCGCCGTCGGAGATAAGGTCGACGATCAGGACGCCATGGATTCCGAAGTAGAGATGATGAAGGAGAGATTTGCAAAGCTTTTGCTGGGAGAAGACATGTCAGGAAGTGGGAAAGGAGTTTGTACGGCGGTTACAATTTCAAACGCCATTACTAATCTTTACGCGACGGTGTTCGGACAGAATCTGAAGCTGGAGCCGTTGCCGCCGGAGAAGAAAGCCATGTGGAAGAGAGAGATGACTTGTTTGTTGTCTGTTTGTGATTACATTGTTGAGTTCTTCACTGAATCTCAAACCTTGGAAGATGGAACAACTATGGAGGTGTTGAACAGCAGACAAAGGTCAGATATATACCTAAACCTTCCAGCATTGCAAAAGCTTGACATGATGCTCTTGGACATTCTGGACAGTTTTCAGGACGCCGAGTTCTGGTACTCGGAAAAAGGAAGCATGTCTTCGGACTCGAATCGTTCGAGATCTGGTTCGTTTCGCCGACTCTCGTTGCAAAAAGAGGAAAAATGGTGGCTGCCAGTTCCCTGCATTCCCTCATGTGGTCTATCGGAGAAGGCGCGAAAACACCTTCGAAATAACCGCGAATGTGCGAACCAAATCCACAAAGCTGCCATGGCAATCAACAGCACTATTCTTGCCGAAATGGAAATTCCAGAGCCATACGTCGAAACTCTTCCAAAGAGTGGAAAGGCGAGTATAGGCGATATGATATACCGATACATGTATGGCGCAGACAAATTCTCACCCGATTATCTGCTCGATTGCTTAAACATAGGATCCGAACACGACGCGCTCGAACTTGCAGACCGAATCGAGGCTTCAATGTATACATGGAGAAGGAAAGCATGTCTAAGCCACTCAAAATCTTCATGGGAATTGGTGAAGGATCTAATGGCTGAAACAGACAAGGGAGACAAGAACACTGTTTTAGCAGAAAGAGCTGAGAGCTTGCTTTTGTCCTTGAAACAAAGGTTCCCTGAACTTTCTCAAACAACCTTAGACACTAGCAAGATTCAATACAATAGGGATGTGGGGCAAGCAGTGTTGGAGAGCTACTCAAGAGTGTTGGAAGGACTGGCATTCAACATTGTGGCTTGGATTGAAGATGTTCTTTTTGCTGATAGGTCCATAAGAAATCAAGAGTAG

Coding sequence (CDS)

ATGGAGAAACTTTCCATCTCTGAGGATAATTTTCGTCTGGGTTTTCGTCCTTCGTCGTCTTCCATGGATCTCAACGACCAATCCACCATTTCTGAAACTTCCGGCCACTCTCCGGCCAGCGGCTACTCTTTCTCTTACTGCAGAACCAGCTCCGACGCCTCCGCCTTCTCCGACCCCACCACCGACGACAGCAGTTTCTGCACTGAGCCCTCTCCGGCCATTCTCGCCAGACAGAGCACCAGAAAATTTAAACTCGCCGTCGGAGATAAGGTCGACGATCAGGACGCCATGGATTCCGAAGTAGAGATGATGAAGGAGAGATTTGCAAAGCTTTTGCTGGGAGAAGACATGTCAGGAAGTGGGAAAGGAGTTTGTACGGCGGTTACAATTTCAAACGCCATTACTAATCTTTACGCGACGGTGTTCGGACAGAATCTGAAGCTGGAGCCGTTGCCGCCGGAGAAGAAAGCCATGTGGAAGAGAGAGATGACTTGTTTGTTGTCTGTTTGTGATTACATTGTTGAGTTCTTCACTGAATCTCAAACCTTGGAAGATGGAACAACTATGGAGGTGTTGAACAGCAGACAAAGGTCAGATATATACCTAAACCTTCCAGCATTGCAAAAGCTTGACATGATGCTCTTGGACATTCTGGACAGTTTTCAGGACGCCGAGTTCTGGTACTCGGAAAAAGGAAGCATGTCTTCGGACTCGAATCGTTCGAGATCTGGTTCGTTTCGCCGACTCTCGTTGCAAAAAGAGGAAAAATGGTGGCTGCCAGTTCCCTGCATTCCCTCATGTGGTCTATCGGAGAAGGCGCGAAAACACCTTCGAAATAACCGCGAATGTGCGAACCAAATCCACAAAGCTGCCATGGCAATCAACAGCACTATTCTTGCCGAAATGGAAATTCCAGAGCCATACGTCGAAACTCTTCCAAAGAGTGGAAAGGCGAGTATAGGCGATATGATATACCGATACATGTATGGCGCAGACAAATTCTCACCCGATTATCTGCTCGATTGCTTAAACATAGGATCCGAACACGACGCGCTCGAACTTGCAGACCGAATCGAGGCTTCAATGTATACATGGAGAAGGAAAGCATGTCTAAGCCACTCAAAATCTTCATGGGAATTGGTGAAGGATCTAATGGCTGAAACAGACAAGGGAGACAAGAACACTGTTTTAGCAGAAAGAGCTGAGAGCTTGCTTTTGTCCTTGAAACAAAGGTTCCCTGAACTTTCTCAAACAACCTTAGACACTAGCAAGATTCAATACAATAGGGATGTGGGGCAAGCAGTGTTGGAGAGCTACTCAAGAGTGTTGGAAGGACTGGCATTCAACATTGTGGCTTGGATTGAAGATGTTCTTTTTGCTGATAGGTCCATAAGAAATCAAGAGTAG

Protein sequence

MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPTTDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMSSDSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE
Homology
BLAST of Lag0016103 vs. NCBI nr
Match: XP_038875284.1 (rop guanine nucleotide exchange factor 3-like [Benincasa hispida])

HSP 1 Score: 855.9 bits (2210), Expect = 1.6e-244
Identity = 431/468 (92.09%), Postives = 451/468 (96.37%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           MEK  IS++NF LGFRPSSSSMDLNDQSTISETSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MEKFPISDENFHLGFRPSSSSMDLNDQSTISETSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS 120
           TDDSSFC EPSPAILARQ TRK+KL+ GDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS
Sbjct: 61  TDDSSFCIEPSPAILARQGTRKYKLSAGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS 120

Query: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTES 180
           GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM CLLSVCDYIVEFFT S
Sbjct: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMCCLLSVCDYIVEFFTIS 180

Query: 181 QTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMSSDSNR 240
           QTLEDGT+MEVL+SRQRSDI LNLPALQKLD+MLLDILDSFQD EFWY+EKGSMSS+SN+
Sbjct: 181 QTLEDGTSMEVLSSRQRSDICLNLPALQKLDLMLLDILDSFQDTEFWYAEKGSMSSNSNQ 240

Query: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILA 300
           S+SGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRN RECANQIHKAAMAINSTILA
Sbjct: 241 SKSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNKRECANQIHKAAMAINSTILA 300

Query: 301 EMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEA 360
           EMEIPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELADRIEA
Sbjct: 301 EMEIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADRIEA 360

Query: 361 SMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTL 420
           SMYTWRRKACLSHSKSSWELVKDLMAETD+ DKNT+LAERAE+LLLSLKQRFPELSQTTL
Sbjct: 361 SMYTWRRKACLSHSKSSWELVKDLMAETDRSDKNTILAERAETLLLSLKQRFPELSQTTL 420

Query: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVL +DRSIRNQE
Sbjct: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLSSDRSIRNQE 468

BLAST of Lag0016103 vs. NCBI nr
Match: XP_004138590.1 (rop guanine nucleotide exchange factor 3 [Cucumis sativus] >KGN45658.1 hypothetical protein Csa_005558 [Cucumis sativus])

HSP 1 Score: 853.6 bits (2204), Expect = 8.1e-244
Identity = 428/468 (91.45%), Postives = 450/468 (96.15%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           M+K SIS++NF LGFRPSSSSMDLND STIS+TSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS 120
           TDD SFCTEPSPAIL RQ TRK+K AVGDK DDQDAMDSE+EMMKERFAKLLLGEDMSGS
Sbjct: 61  TDDGSFCTEPSPAILGRQCTRKYKHAVGDKADDQDAMDSEMEMMKERFAKLLLGEDMSGS 120

Query: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTES 180
           GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM+CLLSVCDYIVEFFT S
Sbjct: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTLS 180

Query: 181 QTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMSSDSNR 240
           QTLEDGT++EVLNSRQRSDI LNLPALQKLDMMLLDILDSFQD EFWY+EKGSMSS+SNR
Sbjct: 181 QTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSMSSNSNR 240

Query: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILA 300
           SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSE ARKHLRN RECANQIHKAAMAINSTILA
Sbjct: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINSTILA 300

Query: 301 EMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEA 360
           EM+IPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELADRIEA
Sbjct: 301 EMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADRIEA 360

Query: 361 SMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTL 420
           SMYTWRRKACLSHSKSSWELVKDLMAET+K DKNT+LAERAE+LLLSLKQRFPELSQTTL
Sbjct: 361 SMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQTTL 420

Query: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLF+DRSIRNQ+
Sbjct: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFSDRSIRNQD 468

BLAST of Lag0016103 vs. NCBI nr
Match: XP_008458234.1 (PREDICTED: rop guanine nucleotide exchange factor 3 isoform X2 [Cucumis melo] >TYK03013.1 rop guanine nucleotide exchange factor 3 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 845.9 bits (2184), Expect = 1.7e-241
Identity = 425/468 (90.81%), Postives = 448/468 (95.73%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           M+K SIS++NF LGFRPSSSSMDLND STIS+TSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS 120
           TDDSSF TEPSPAIL RQ TRK+K A GDK DD DAMDSE+EMMKERFAKLLLGEDMSGS
Sbjct: 61  TDDSSFFTEPSPAILGRQCTRKYKHAAGDKADDHDAMDSEMEMMKERFAKLLLGEDMSGS 120

Query: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTES 180
           GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM+CLLSVCDYIVEFFT S
Sbjct: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTLS 180

Query: 181 QTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMSSDSNR 240
           QTLEDGT++EVLNSRQRSDI LNLPALQKLDMMLLDILDSFQD EFWY+EKGS+SS+SNR
Sbjct: 181 QTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSVSSNSNR 240

Query: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILA 300
           SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSE ARKHLRN RECANQIHKAAMAINSTILA
Sbjct: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINSTILA 300

Query: 301 EMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEA 360
           EM+IPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELADRIEA
Sbjct: 301 EMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADRIEA 360

Query: 361 SMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTL 420
           SMYTWRRKACLSHSKSSWELVKDLMAET+K DKNT+LAERAE+LLLSLKQRFPELSQTTL
Sbjct: 361 SMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQTTL 420

Query: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLF+DRSIRNQ+
Sbjct: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFSDRSIRNQD 468

BLAST of Lag0016103 vs. NCBI nr
Match: XP_008458233.1 (PREDICTED: rop guanine nucleotide exchange factor 3 isoform X1 [Cucumis melo])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 425/473 (89.85%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           M+K SIS++NF LGFRPSSSSMDLND STIS+TSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDS-----EVEMMKERFAKLLLGE 120
           TDDSSF TEPSPAIL RQ TRK+K A GDK DD DAMDS     E+EMMKERFAKLLLGE
Sbjct: 61  TDDSSFFTEPSPAILGRQCTRKYKHAAGDKADDHDAMDSVVVITEMEMMKERFAKLLLGE 120

Query: 121 DMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVE 180
           DMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM+CLLSVCDYIVE
Sbjct: 121 DMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVE 180

Query: 181 FFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMS 240
           FFT SQTLEDGT++EVLNSRQRSDI LNLPALQKLDMMLLDILDSFQD EFWY+EKGS+S
Sbjct: 181 FFTLSQTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSVS 240

Query: 241 SDSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAIN 300
           S+SNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSE ARKHLRN RECANQIHKAAMAIN
Sbjct: 241 SNSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAIN 300

Query: 301 STILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELA 360
           STILAEM+IPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELA
Sbjct: 301 STILAEMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELA 360

Query: 361 DRIEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPEL 420
           DRIEASMYTWRRKACLSHSKSSWELVKDLMAET+K DKNT+LAERAE+LLLSLKQRFPEL
Sbjct: 361 DRIEASMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPEL 420

Query: 421 SQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           SQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLF+DRSIRNQ+
Sbjct: 421 SQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFSDRSIRNQD 473

BLAST of Lag0016103 vs. NCBI nr
Match: XP_023000613.1 (rop guanine nucleotide exchange factor 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 810.8 bits (2093), Expect = 6.0e-231
Identity = 415/467 (88.87%), Postives = 434/467 (92.93%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPAS-GYSFSYCRTSSDASAFSDP 60
           MEK SIS+  F LGF PSSSSMDLND ST+SETSG SP S  YSFSYCRTSSDASAFSDP
Sbjct: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60

Query: 61  TTDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSG 120
           TTDD+SFCTEPSPAI     TRK+KL  GDK DDQDAMDSE EMMKERFAKLLLGEDMSG
Sbjct: 61  TTDDTSFCTEPSPAI----PTRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSG 120

Query: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTE 180
           SGKGVCTAVTISNAITNLYATVFGQNLKLEPLP EKKAMWKREM CLLSVCDYI+EFFTE
Sbjct: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTE 180

Query: 181 SQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMS--SD 240
           SQTL+DGTTMEVLNSRQRSDIYLNLPAL+KLDMMLLDILDSF D EFWY+EKGSMS  S+
Sbjct: 181 SQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSN 240

Query: 241 SNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINST 300
           SNRS+S SFRRLSLQKEEKWWLPVPC+PSCGLSEKARK LRNNRE ANQIHKAAMAINST
Sbjct: 241 SNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINST 300

Query: 301 ILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADR 360
           ILAEM+IPE Y++TLPKSGK SIGD +YRYMY ADKFSPD+LLDCLNIGSEHDALELADR
Sbjct: 301 ILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADR 360

Query: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQ 420
           IEASMYTWRRKACLSHSKSSWELVKDLMAE DKGDKNT+LAERAESLLLSLKQRFPELSQ
Sbjct: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQ 420

Query: 421 TTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSI 465
           TTLDTSKIQYNRDVGQAVLESYSRVLEGLAFN+VAWIEDVLFADRS+
Sbjct: 421 TTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNVVAWIEDVLFADRSL 463

BLAST of Lag0016103 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 1.9e-163
Identity = 303/477 (63.52%), Postives = 374/477 (78.41%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSD-ASAFSDP 60
           ME LS  ++N       S  S+D NDQS + ET  +S  S  SF Y RT S+  S FSD 
Sbjct: 1   MENLSNPDEN---DDHQSPRSIDQNDQSAV-ETPVYSTMSIDSFVYPRTCSETTSGFSDQ 60

Query: 61  TTDDSSFCTEPSPA---ILARQSTRK----FKLAVGDKVDDQDAMDSEVEMMKERFAKLL 120
             + +SFC+E SP    +L    + K     ++   + +  Q+  + E+E MKERFAKLL
Sbjct: 61  IDETNSFCSEASPCNWPVLTESKSSKCLSGLEMQSNECLVVQEISEPELETMKERFAKLL 120

Query: 121 LGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDY 180
           LGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  EK+A+WKREM CLLSVCDY
Sbjct: 121 LGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKREMNCLLSVCDY 180

Query: 181 IVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKG 240
           IVEF    Q L +G T+EV+ SR R+DIY+NLPAL+KLD ML++ LDSFQ+ EFWY+E+G
Sbjct: 181 IVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQNTEFWYAEEG 240

Query: 241 SMSSDSNRSRSGSFRRLSLQ-KEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAA 300
           S+S  S RS +GSFR++ +Q KEEKWWLPVP +PS GLS+KARK L+N RE  NQIHKAA
Sbjct: 241 SLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKRESTNQIHKAA 300

Query: 301 MAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDA 360
           MAINS+IL+EMEIP+ Y+ TLPK GK+S+GD IYRYM G+ +F P+ LLDCLNI SEH+A
Sbjct: 301 MAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLDCLNISSEHEA 360

Query: 361 LELADRIEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQR 420
           ++LADR+EASMYTWRRK+CLS+SK+SW +VKDLM+ T++ DKN V+AERAE+LL  LKQR
Sbjct: 361 VQLADRVEASMYTWRRKSCLSNSKNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQR 420

Query: 421 FPELSQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           +PELSQT+LD  KIQYN+DVG+AVLESYSRVLEGLAFNIVAWI+DVL+ D+++R  E
Sbjct: 421 YPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLYVDKTMRGSE 473

BLAST of Lag0016103 vs. ExPASy Swiss-Prot
Match: Q9LQ89 (Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF2 PE=1 SV=2)

HSP 1 Score: 563.5 bits (1451), Expect = 2.2e-159
Identity = 300/486 (61.73%), Postives = 369/486 (75.93%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDAS--AFSD 60
           ME L   E+N  +G+  S   +D ND S  SET  +S  S  SF+Y RT S+ S   FSD
Sbjct: 1   MENLPNHEENDDVGYHQSPGPIDPNDHSA-SETPVYSTMSTDSFAYHRTCSETSGGGFSD 60

Query: 61  PTTDDSSFCTEPSPA---ILARQST------------RKFKLAVGDKVDDQDAMDSEVEM 120
              + SSFCTE SP+   +L   +             +  ++   + +  Q+  + E+E 
Sbjct: 61  QIDETSSFCTEASPSDWPVLTESNNSASSNFPTVFDLKHNQIETDEHLAVQEISEPELET 120

Query: 121 MKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM 180
           MKERF+KLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  E+K  WKREM
Sbjct: 121 MKERFSKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLEIEQKTTWKREM 180

Query: 181 TCLLSVCDYIVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQD 240
            CLLSVCDYI EF  +SQ L +G T+EV+ SR R+DIY+NLPAL+KLD ML++ LDSFQ 
Sbjct: 181 NCLLSVCDYIFEFIPKSQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQK 240

Query: 241 AEFWYSEKGSMSSDSNRSRSGSFRRLSLQ-KEEKWWLPVPCIPSCGLSEKARKHLRNNRE 300
            EFWY+E+GS+S  S RS +GSFR++ +Q KEEKWWLP+P +P  GLSEKARK L++ RE
Sbjct: 241 TEFWYAEEGSLSMKSTRSATGSFRKVIVQRKEEKWWLPIPLVPLQGLSEKARKQLKSKRE 300

Query: 301 CANQIHKAAMAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDC 360
             NQIHKAAMAINS+IL EM+IP+ Y+ TLPKSGKAS GD IYR+M  + +FSP+ LLD 
Sbjct: 301 STNQIHKAAMAINSSILGEMDIPDSYMATLPKSGKASTGDAIYRHMTSSGRFSPEKLLDR 360

Query: 361 LNIGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAE 420
           L I SEH+AL+LADR+EASMYTWRRKACL++SKSSW +VKDLM+ T++ DKN VLAERAE
Sbjct: 361 LKIVSEHEALQLADRVEASMYTWRRKACLNNSKSSWNMVKDLMSITERSDKNYVLAERAE 420

Query: 421 SLLLSLKQRFPELSQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADR 469
           SLL  LKQR+PELSQT+LD  KI  N+DVG+AVLESYSRVLEGLAFNIVAWI+DVL+ D+
Sbjct: 421 SLLFCLKQRYPELSQTSLDICKIHCNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLYVDK 480

BLAST of Lag0016103 vs. ExPASy Swiss-Prot
Match: Q0WNP7 (Rop guanine nucleotide exchange factor 4 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF4 PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.1e-137
Identity = 263/466 (56.44%), Postives = 342/466 (73.39%), Query Frame = 0

Query: 26  DQSTISETSGHSPASGYSFSYCRTSSDASAFSDPTTDDSSFCTEPSPAILARQSTRKFKL 85
           + S+ S+ +  +P S  S  Y RT SD S  S    D  SF   PS       S  +  +
Sbjct: 2   ESSSNSDQNEGTPTSSVSSPYRRTYSDISGLSH-RFDVQSFYNRPSNTNAVVLSGHEEDV 61

Query: 86  AV----------------GDKVDD--QDAMDSEVEMMKERFAKLLLGEDMSGSGKGVCTA 145
           +                 GD+ D     A D+E+EMM+ERFAKLLLGEDMSGSGKGVCTA
Sbjct: 62  SEDAEEPKDDVVNDVHGDGDEEDSDIDSAEDAELEMMRERFAKLLLGEDMSGSGKGVCTA 121

Query: 146 VTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTESQTLEDGT 205
           VT+SN+ITNLYATVFGQ+L+L+PL  EKK +WKREM C +S+CDYIVE    S     GT
Sbjct: 122 VTVSNSITNLYATVFGQSLRLQPLSTEKKDLWKREMNCFMSICDYIVEVIPRSL----GT 181

Query: 206 TMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKG--SMSSDSNRSRSGS 265
            +E+  ++ RSDI ++LPAL+KLD ML++ILDSF + EFWY E+G  SM+S      SG+
Sbjct: 182 NVEITETKLRSDILMSLPALRKLDNMLMEILDSFTENEFWYVERGSSSMNSGGGGRDSGT 241

Query: 266 FRRLSLQ-KEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILAEMEI 325
           FR++ +Q K+EKWWLPVPC+P+ GLSE+ RKHLR+ R+CA+QIHKAA+AIN + L +M+I
Sbjct: 242 FRKVVVQRKDEKWWLPVPCVPAEGLSEEERKHLRHKRDCASQIHKAALAINDSTLNDMDI 301

Query: 326 PEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEASMYT 385
           P+ Y+ TLPKSGKAS+GD+IY+ +  A+KF PD LLD L I SEH+ALELAD++EAS+ T
Sbjct: 302 PDSYLTTLPKSGKASVGDVIYKQLCTAEKFYPDQLLDILKITSEHEALELADKVEASLVT 361

Query: 386 WRRK-ACLSHSKSSWELVKDLMAETDKG-DKNTVLAERAESLLLSLKQRFPELSQTTLDT 445
           WRRK   L+HSKSSW+++KD+  + D+G DKN +LA RA SLL  LKQR+PELSQT+LD 
Sbjct: 362 WRRKTGGLTHSKSSWDMMKDISGDADRGNDKNHILAARARSLLFCLKQRYPELSQTSLDI 421

Query: 446 SKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
            KIQ+NRDVG+AVLESYSRVLEGLA+N+V+WI+DVL+ DR++RN++
Sbjct: 422 CKIQFNRDVGKAVLESYSRVLEGLAYNVVSWIDDVLYVDRTVRNRD 462

BLAST of Lag0016103 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.5e-123
Identity = 237/426 (55.63%), Postives = 314/426 (73.71%), Query Frame = 0

Query: 52  DASAFSDPTT-----DDSSFCTEPSPA--ILARQSTRKFKLAVGDKVD-DQDAMDSEVEM 111
           ++S  S  TT     + S  CTE   A  + +R S +      G+K     D+  SEVEM
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNID---GEKKKIRSDSRVSEVEM 76

Query: 112 MKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM 171
           MKERF+KLLLGEDMSGSG GVCTA+ ISNAITNL AT+FGQ  +LEPLP EKK MW+REM
Sbjct: 77  MKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEKKEMWRREM 136

Query: 172 TCLLSVCDYIVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQD 231
             LL V D+IVE     QT  DGT +E++  R RSD+Y+NLPAL+KLD MLL+ILDSF++
Sbjct: 137 EWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLLEILDSFEE 196

Query: 232 AEFWYSEKGSMSSDSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNREC 291
            EFWY ++G M+ +S    S SFR+   ++E+KWWLPVP +   GL E +RK L++ R+C
Sbjct: 197 TEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRKQLQHKRDC 256

Query: 292 ANQIHKAAMAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCL 351
            NQI KAAMAINS  LA+MEIPE Y+E+LP+ G++ +GD+IYRY+  +D+FSP+ LLDCL
Sbjct: 257 TNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYI-SSDQFSPECLLDCL 316

Query: 352 NIGSEHDALELADRIEASMYTWRRKA---CLSHSKSSWELVKDLMAETDKGDKNTVLAER 411
           ++ SEH A+E+A+R+E+S+Y W ++      +++K+SWE+VK+LM + DK +   ++A+R
Sbjct: 317 DLSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELMVDADKLE---LMADR 376

Query: 412 AESLLLSLKQRFPELSQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFA 467
           AESLLLSLKQRFP L QT LD SKIQYN+D+G+++LESYSRVLE LAFNIVA I+D+LF 
Sbjct: 377 AESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFNIVARIDDLLFV 435

BLAST of Lag0016103 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 418.3 bits (1074), Expect = 1.1e-115
Identity = 221/384 (57.55%), Postives = 286/384 (74.48%), Query Frame = 0

Query: 89  DKVDDQDAMDSEVEMMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKL 148
           DK  D D   SE+EMMKERFAKLLLGEDMSG GKGVCTA+ ISNAITNL ATVFG+  +L
Sbjct: 81  DKQPDNDL--SEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRL 140

Query: 149 EPLPPEKKAMWKREMTCLLSVCDYIVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQ 208
           EPL P+KKAMW+RE+  LL V D IVE     Q    G T E++ +R RSD+Y NLPAL+
Sbjct: 141 EPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALK 200

Query: 209 KLDMMLLDILDSFQDAEFWYSEKGSMSSDSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCG 268
           KLD ML+D+LD+F D EFWY+++G +  D ++    S    S+++E+KWWLP P +P  G
Sbjct: 201 KLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNS--PASVRQEDKWWLPCPKVPPNG 260

Query: 269 LSEKARKHLRNNRECANQIHKAAMAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYM 328
           LSE+ARK L+  R+ ANQI KAA+AINS +LAEMEIP+PY+ETLPKSGK  +G++IY+Y+
Sbjct: 261 LSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYL 320

Query: 329 YGADKFSPDYLLDCLNIGSEHDALELADRIEASMYTWRRKACLSHSK------SSW-ELV 388
             A+KFSP+ LLDCL++ SEH  LE+A+RIEA+++ WR+K    H K      SSW   V
Sbjct: 321 -TANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKKQAKLKLSSWGGKV 380

Query: 389 KDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTLDTSKIQYNRDVGQAVLESYSR 448
           K L+ + ++ D    L +RAE+LL SL+ RFP L QTTLD +KIQYN+DVGQ++LESYSR
Sbjct: 381 KGLVNDNERND---FLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSR 440

Query: 449 VLEGLAFNIVAWIEDVLFADRSIR 466
           V+E +AFNI A I+DVL+ D ++R
Sbjct: 441 VMESMAFNITARIDDVLYVDDAMR 456

BLAST of Lag0016103 vs. ExPASy TrEMBL
Match: A0A0A0K849 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G004490 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.9e-244
Identity = 428/468 (91.45%), Postives = 450/468 (96.15%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           M+K SIS++NF LGFRPSSSSMDLND STIS+TSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS 120
           TDD SFCTEPSPAIL RQ TRK+K AVGDK DDQDAMDSE+EMMKERFAKLLLGEDMSGS
Sbjct: 61  TDDGSFCTEPSPAILGRQCTRKYKHAVGDKADDQDAMDSEMEMMKERFAKLLLGEDMSGS 120

Query: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTES 180
           GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM+CLLSVCDYIVEFFT S
Sbjct: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTLS 180

Query: 181 QTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMSSDSNR 240
           QTLEDGT++EVLNSRQRSDI LNLPALQKLDMMLLDILDSFQD EFWY+EKGSMSS+SNR
Sbjct: 181 QTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSMSSNSNR 240

Query: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILA 300
           SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSE ARKHLRN RECANQIHKAAMAINSTILA
Sbjct: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINSTILA 300

Query: 301 EMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEA 360
           EM+IPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELADRIEA
Sbjct: 301 EMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADRIEA 360

Query: 361 SMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTL 420
           SMYTWRRKACLSHSKSSWELVKDLMAET+K DKNT+LAERAE+LLLSLKQRFPELSQTTL
Sbjct: 361 SMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQTTL 420

Query: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLF+DRSIRNQ+
Sbjct: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFSDRSIRNQD 468

BLAST of Lag0016103 vs. ExPASy TrEMBL
Match: A0A5D3BTM4 (Rop guanine nucleotide exchange factor 3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001080 PE=4 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.1e-242
Identity = 425/468 (90.81%), Postives = 448/468 (95.73%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           M+K SIS++NF LGFRPSSSSMDLND STIS+TSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS 120
           TDDSSF TEPSPAIL RQ TRK+K A GDK DD DAMDSE+EMMKERFAKLLLGEDMSGS
Sbjct: 61  TDDSSFFTEPSPAILGRQCTRKYKHAAGDKADDHDAMDSEMEMMKERFAKLLLGEDMSGS 120

Query: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTES 180
           GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM+CLLSVCDYIVEFFT S
Sbjct: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTLS 180

Query: 181 QTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMSSDSNR 240
           QTLEDGT++EVLNSRQRSDI LNLPALQKLDMMLLDILDSFQD EFWY+EKGS+SS+SNR
Sbjct: 181 QTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSVSSNSNR 240

Query: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILA 300
           SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSE ARKHLRN RECANQIHKAAMAINSTILA
Sbjct: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINSTILA 300

Query: 301 EMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEA 360
           EM+IPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELADRIEA
Sbjct: 301 EMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADRIEA 360

Query: 361 SMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTL 420
           SMYTWRRKACLSHSKSSWELVKDLMAET+K DKNT+LAERAE+LLLSLKQRFPELSQTTL
Sbjct: 361 SMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQTTL 420

Query: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLF+DRSIRNQ+
Sbjct: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFSDRSIRNQD 468

BLAST of Lag0016103 vs. ExPASy TrEMBL
Match: A0A1S3C7D5 (rop guanine nucleotide exchange factor 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497718 PE=4 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.1e-242
Identity = 425/468 (90.81%), Postives = 448/468 (95.73%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           M+K SIS++NF LGFRPSSSSMDLND STIS+TSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSGS 120
           TDDSSF TEPSPAIL RQ TRK+K A GDK DD DAMDSE+EMMKERFAKLLLGEDMSGS
Sbjct: 61  TDDSSFFTEPSPAILGRQCTRKYKHAAGDKADDHDAMDSEMEMMKERFAKLLLGEDMSGS 120

Query: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTES 180
           GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM+CLLSVCDYIVEFFT S
Sbjct: 121 GKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTLS 180

Query: 181 QTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMSSDSNR 240
           QTLEDGT++EVLNSRQRSDI LNLPALQKLDMMLLDILDSFQD EFWY+EKGS+SS+SNR
Sbjct: 181 QTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSVSSNSNR 240

Query: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILA 300
           SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSE ARKHLRN RECANQIHKAAMAINSTILA
Sbjct: 241 SRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINSTILA 300

Query: 301 EMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEA 360
           EM+IPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELADRIEA
Sbjct: 301 EMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADRIEA 360

Query: 361 SMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTL 420
           SMYTWRRKACLSHSKSSWELVKDLMAET+K DKNT+LAERAE+LLLSLKQRFPELSQTTL
Sbjct: 361 SMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQTTL 420

Query: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLF+DRSIRNQ+
Sbjct: 421 DTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFSDRSIRNQD 468

BLAST of Lag0016103 vs. ExPASy TrEMBL
Match: A0A1S3C7I2 (rop guanine nucleotide exchange factor 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497718 PE=4 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.8e-240
Identity = 425/473 (89.85%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDASAFSDPT 60
           M+K SIS++NF LGFRPSSSSMDLND STIS+TSG SPASGYSFSYCRTSSDASAFSDPT
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPASGYSFSYCRTSSDASAFSDPT 60

Query: 61  TDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDS-----EVEMMKERFAKLLLGE 120
           TDDSSF TEPSPAIL RQ TRK+K A GDK DD DAMDS     E+EMMKERFAKLLLGE
Sbjct: 61  TDDSSFFTEPSPAILGRQCTRKYKHAAGDKADDHDAMDSVVVITEMEMMKERFAKLLLGE 120

Query: 121 DMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVE 180
           DMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM+CLLSVCDYIVE
Sbjct: 121 DMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVE 180

Query: 181 FFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMS 240
           FFT SQTLEDGT++EVLNSRQRSDI LNLPALQKLDMMLLDILDSFQD EFWY+EKGS+S
Sbjct: 181 FFTLSQTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSVS 240

Query: 241 SDSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAIN 300
           S+SNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSE ARKHLRN RECANQIHKAAMAIN
Sbjct: 241 SNSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAIN 300

Query: 301 STILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELA 360
           STILAEM+IPE +++TLPKSGKASIGD IYRYMY ADKFSP++LLDCLNIGSEHDALELA
Sbjct: 301 STILAEMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELA 360

Query: 361 DRIEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPEL 420
           DRIEASMYTWRRKACLSHSKSSWELVKDLMAET+K DKNT+LAERAE+LLLSLKQRFPEL
Sbjct: 361 DRIEASMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPEL 420

Query: 421 SQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           SQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLF+DRSIRNQ+
Sbjct: 421 SQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFSDRSIRNQD 473

BLAST of Lag0016103 vs. ExPASy TrEMBL
Match: A0A6J1KN41 (rop guanine nucleotide exchange factor 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111494853 PE=4 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.9e-231
Identity = 415/467 (88.87%), Postives = 434/467 (92.93%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPAS-GYSFSYCRTSSDASAFSDP 60
           MEK SIS+  F LGF PSSSSMDLND ST+SETSG SP S  YSFSYCRTSSDASAFSDP
Sbjct: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60

Query: 61  TTDDSSFCTEPSPAILARQSTRKFKLAVGDKVDDQDAMDSEVEMMKERFAKLLLGEDMSG 120
           TTDD+SFCTEPSPAI     TRK+KL  GDK DDQDAMDSE EMMKERFAKLLLGEDMSG
Sbjct: 61  TTDDTSFCTEPSPAI----PTRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSG 120

Query: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTE 180
           SGKGVCTAVTISNAITNLYATVFGQNLKLEPLP EKKAMWKREM CLLSVCDYI+EFFTE
Sbjct: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTE 180

Query: 181 SQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKGSMS--SD 240
           SQTL+DGTTMEVLNSRQRSDIYLNLPAL+KLDMMLLDILDSF D EFWY+EKGSMS  S+
Sbjct: 181 SQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSN 240

Query: 241 SNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINST 300
           SNRS+S SFRRLSLQKEEKWWLPVPC+PSCGLSEKARK LRNNRE ANQIHKAAMAINST
Sbjct: 241 SNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINST 300

Query: 301 ILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADR 360
           ILAEM+IPE Y++TLPKSGK SIGD +YRYMY ADKFSPD+LLDCLNIGSEHDALELADR
Sbjct: 301 ILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADR 360

Query: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQ 420
           IEASMYTWRRKACLSHSKSSWELVKDLMAE DKGDKNT+LAERAESLLLSLKQRFPELSQ
Sbjct: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQ 420

Query: 421 TTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSI 465
           TTLDTSKIQYNRDVGQAVLESYSRVLEGLAFN+VAWIEDVLFADRS+
Sbjct: 421 TTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNVVAWIEDVLFADRSL 463

BLAST of Lag0016103 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 577.0 bits (1486), Expect = 1.4e-164
Identity = 303/477 (63.52%), Postives = 374/477 (78.41%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSD-ASAFSDP 60
           ME LS  ++N       S  S+D NDQS + ET  +S  S  SF Y RT S+  S FSD 
Sbjct: 1   MENLSNPDEN---DDHQSPRSIDQNDQSAV-ETPVYSTMSIDSFVYPRTCSETTSGFSDQ 60

Query: 61  TTDDSSFCTEPSPA---ILARQSTRK----FKLAVGDKVDDQDAMDSEVEMMKERFAKLL 120
             + +SFC+E SP    +L    + K     ++   + +  Q+  + E+E MKERFAKLL
Sbjct: 61  IDETNSFCSEASPCNWPVLTESKSSKCLSGLEMQSNECLVVQEISEPELETMKERFAKLL 120

Query: 121 LGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDY 180
           LGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  EK+A+WKREM CLLSVCDY
Sbjct: 121 LGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKREMNCLLSVCDY 180

Query: 181 IVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKG 240
           IVEF    Q L +G T+EV+ SR R+DIY+NLPAL+KLD ML++ LDSFQ+ EFWY+E+G
Sbjct: 181 IVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQNTEFWYAEEG 240

Query: 241 SMSSDSNRSRSGSFRRLSLQ-KEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAA 300
           S+S  S RS +GSFR++ +Q KEEKWWLPVP +PS GLS+KARK L+N RE  NQIHKAA
Sbjct: 241 SLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKRESTNQIHKAA 300

Query: 301 MAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDA 360
           MAINS+IL+EMEIP+ Y+ TLPK GK+S+GD IYRYM G+ +F P+ LLDCLNI SEH+A
Sbjct: 301 MAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLDCLNISSEHEA 360

Query: 361 LELADRIEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAESLLLSLKQR 420
           ++LADR+EASMYTWRRK+CLS+SK+SW +VKDLM+ T++ DKN V+AERAE+LL  LKQR
Sbjct: 361 VQLADRVEASMYTWRRKSCLSNSKNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQR 420

Query: 421 FPELSQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
           +PELSQT+LD  KIQYN+DVG+AVLESYSRVLEGLAFNIVAWI+DVL+ D+++R  E
Sbjct: 421 YPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLYVDKTMRGSE 473

BLAST of Lag0016103 vs. TAIR 10
Match: AT1G01700.1 (RHO guanyl-nucleotide exchange factor 2 )

HSP 1 Score: 563.5 bits (1451), Expect = 1.6e-160
Identity = 300/486 (61.73%), Postives = 369/486 (75.93%), Query Frame = 0

Query: 1   MEKLSISEDNFRLGFRPSSSSMDLNDQSTISETSGHSPASGYSFSYCRTSSDAS--AFSD 60
           ME L   E+N  +G+  S   +D ND S  SET  +S  S  SF+Y RT S+ S   FSD
Sbjct: 1   MENLPNHEENDDVGYHQSPGPIDPNDHSA-SETPVYSTMSTDSFAYHRTCSETSGGGFSD 60

Query: 61  PTTDDSSFCTEPSPA---ILARQST------------RKFKLAVGDKVDDQDAMDSEVEM 120
              + SSFCTE SP+   +L   +             +  ++   + +  Q+  + E+E 
Sbjct: 61  QIDETSSFCTEASPSDWPVLTESNNSASSNFPTVFDLKHNQIETDEHLAVQEISEPELET 120

Query: 121 MKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM 180
           MKERF+KLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  E+K  WKREM
Sbjct: 121 MKERFSKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLEIEQKTTWKREM 180

Query: 181 TCLLSVCDYIVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQD 240
            CLLSVCDYI EF  +SQ L +G T+EV+ SR R+DIY+NLPAL+KLD ML++ LDSFQ 
Sbjct: 181 NCLLSVCDYIFEFIPKSQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQK 240

Query: 241 AEFWYSEKGSMSSDSNRSRSGSFRRLSLQ-KEEKWWLPVPCIPSCGLSEKARKHLRNNRE 300
            EFWY+E+GS+S  S RS +GSFR++ +Q KEEKWWLP+P +P  GLSEKARK L++ RE
Sbjct: 241 TEFWYAEEGSLSMKSTRSATGSFRKVIVQRKEEKWWLPIPLVPLQGLSEKARKQLKSKRE 300

Query: 301 CANQIHKAAMAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDC 360
             NQIHKAAMAINS+IL EM+IP+ Y+ TLPKSGKAS GD IYR+M  + +FSP+ LLD 
Sbjct: 301 STNQIHKAAMAINSSILGEMDIPDSYMATLPKSGKASTGDAIYRHMTSSGRFSPEKLLDR 360

Query: 361 LNIGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAETDKGDKNTVLAERAE 420
           L I SEH+AL+LADR+EASMYTWRRKACL++SKSSW +VKDLM+ T++ DKN VLAERAE
Sbjct: 361 LKIVSEHEALQLADRVEASMYTWRRKACLNNSKSSWNMVKDLMSITERSDKNYVLAERAE 420

Query: 421 SLLLSLKQRFPELSQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADR 469
           SLL  LKQR+PELSQT+LD  KI  N+DVG+AVLESYSRVLEGLAFNIVAWI+DVL+ D+
Sbjct: 421 SLLFCLKQRYPELSQTSLDICKIHCNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLYVDK 480

BLAST of Lag0016103 vs. TAIR 10
Match: AT2G45890.1 (RHO guanyl-nucleotide exchange factor 4 )

HSP 1 Score: 490.0 bits (1260), Expect = 2.2e-138
Identity = 263/466 (56.44%), Postives = 342/466 (73.39%), Query Frame = 0

Query: 26  DQSTISETSGHSPASGYSFSYCRTSSDASAFSDPTTDDSSFCTEPSPAILARQSTRKFKL 85
           + S+ S+ +  +P S  S  Y RT SD S  S    D  SF   PS       S  +  +
Sbjct: 2   ESSSNSDQNEGTPTSSVSSPYRRTYSDISGLSH-RFDVQSFYNRPSNTNAVVLSGHEEDV 61

Query: 86  AV----------------GDKVDD--QDAMDSEVEMMKERFAKLLLGEDMSGSGKGVCTA 145
           +                 GD+ D     A D+E+EMM+ERFAKLLLGEDMSGSGKGVCTA
Sbjct: 62  SEDAEEPKDDVVNDVHGDGDEEDSDIDSAEDAELEMMRERFAKLLLGEDMSGSGKGVCTA 121

Query: 146 VTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMTCLLSVCDYIVEFFTESQTLEDGT 205
           VT+SN+ITNLYATVFGQ+L+L+PL  EKK +WKREM C +S+CDYIVE    S     GT
Sbjct: 122 VTVSNSITNLYATVFGQSLRLQPLSTEKKDLWKREMNCFMSICDYIVEVIPRSL----GT 181

Query: 206 TMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQDAEFWYSEKG--SMSSDSNRSRSGS 265
            +E+  ++ RSDI ++LPAL+KLD ML++ILDSF + EFWY E+G  SM+S      SG+
Sbjct: 182 NVEITETKLRSDILMSLPALRKLDNMLMEILDSFTENEFWYVERGSSSMNSGGGGRDSGT 241

Query: 266 FRRLSLQ-KEEKWWLPVPCIPSCGLSEKARKHLRNNRECANQIHKAAMAINSTILAEMEI 325
           FR++ +Q K+EKWWLPVPC+P+ GLSE+ RKHLR+ R+CA+QIHKAA+AIN + L +M+I
Sbjct: 242 FRKVVVQRKDEKWWLPVPCVPAEGLSEEERKHLRHKRDCASQIHKAALAINDSTLNDMDI 301

Query: 326 PEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCLNIGSEHDALELADRIEASMYT 385
           P+ Y+ TLPKSGKAS+GD+IY+ +  A+KF PD LLD L I SEH+ALELAD++EAS+ T
Sbjct: 302 PDSYLTTLPKSGKASVGDVIYKQLCTAEKFYPDQLLDILKITSEHEALELADKVEASLVT 361

Query: 386 WRRK-ACLSHSKSSWELVKDLMAETDKG-DKNTVLAERAESLLLSLKQRFPELSQTTLDT 445
           WRRK   L+HSKSSW+++KD+  + D+G DKN +LA RA SLL  LKQR+PELSQT+LD 
Sbjct: 362 WRRKTGGLTHSKSSWDMMKDISGDADRGNDKNHILAARARSLLFCLKQRYPELSQTSLDI 421

Query: 446 SKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFADRSIRNQE 469
            KIQ+NRDVG+AVLESYSRVLEGLA+N+V+WI+DVL+ DR++RN++
Sbjct: 422 CKIQFNRDVGKAVLESYSRVLEGLAYNVVSWIDDVLYVDRTVRNRD 462

BLAST of Lag0016103 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-124
Identity = 237/426 (55.63%), Postives = 314/426 (73.71%), Query Frame = 0

Query: 52  DASAFSDPTT-----DDSSFCTEPSPA--ILARQSTRKFKLAVGDKVD-DQDAMDSEVEM 111
           ++S  S  TT     + S  CTE   A  + +R S +      G+K     D+  SEVEM
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNID---GEKKKIRSDSRVSEVEM 76

Query: 112 MKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREM 171
           MKERF+KLLLGEDMSGSG GVCTA+ ISNAITNL AT+FGQ  +LEPLP EKK MW+REM
Sbjct: 77  MKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEKKEMWRREM 136

Query: 172 TCLLSVCDYIVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQKLDMMLLDILDSFQD 231
             LL V D+IVE     QT  DGT +E++  R RSD+Y+NLPAL+KLD MLL+ILDSF++
Sbjct: 137 EWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLLEILDSFEE 196

Query: 232 AEFWYSEKGSMSSDSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSEKARKHLRNNREC 291
            EFWY ++G M+ +S    S SFR+   ++E+KWWLPVP +   GL E +RK L++ R+C
Sbjct: 197 TEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRKQLQHKRDC 256

Query: 292 ANQIHKAAMAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYMYGADKFSPDYLLDCL 351
            NQI KAAMAINS  LA+MEIPE Y+E+LP+ G++ +GD+IYRY+  +D+FSP+ LLDCL
Sbjct: 257 TNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYI-SSDQFSPECLLDCL 316

Query: 352 NIGSEHDALELADRIEASMYTWRRKA---CLSHSKSSWELVKDLMAETDKGDKNTVLAER 411
           ++ SEH A+E+A+R+E+S+Y W ++      +++K+SWE+VK+LM + DK +   ++A+R
Sbjct: 317 DLSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELMVDADKLE---LMADR 376

Query: 412 AESLLLSLKQRFPELSQTTLDTSKIQYNRDVGQAVLESYSRVLEGLAFNIVAWIEDVLFA 467
           AESLLLSLKQRFP L QT LD SKIQYN+D+G+++LESYSRVLE LAFNIVA I+D+LF 
Sbjct: 377 AESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFNIVARIDDLLFV 435

BLAST of Lag0016103 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 418.3 bits (1074), Expect = 8.1e-117
Identity = 221/384 (57.55%), Postives = 286/384 (74.48%), Query Frame = 0

Query: 89  DKVDDQDAMDSEVEMMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKL 148
           DK  D D   SE+EMMKERFAKLLLGEDMSG GKGVCTA+ ISNAITNL ATVFG+  +L
Sbjct: 81  DKQPDNDL--SEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRL 140

Query: 149 EPLPPEKKAMWKREMTCLLSVCDYIVEFFTESQTLEDGTTMEVLNSRQRSDIYLNLPALQ 208
           EPL P+KKAMW+RE+  LL V D IVE     Q    G T E++ +R RSD+Y NLPAL+
Sbjct: 141 EPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALK 200

Query: 209 KLDMMLLDILDSFQDAEFWYSEKGSMSSDSNRSRSGSFRRLSLQKEEKWWLPVPCIPSCG 268
           KLD ML+D+LD+F D EFWY+++G +  D ++    S    S+++E+KWWLP P +P  G
Sbjct: 201 KLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNS--PASVRQEDKWWLPCPKVPPNG 260

Query: 269 LSEKARKHLRNNRECANQIHKAAMAINSTILAEMEIPEPYVETLPKSGKASIGDMIYRYM 328
           LSE+ARK L+  R+ ANQI KAA+AINS +LAEMEIP+PY+ETLPKSGK  +G++IY+Y+
Sbjct: 261 LSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYL 320

Query: 329 YGADKFSPDYLLDCLNIGSEHDALELADRIEASMYTWRRKACLSHSK------SSW-ELV 388
             A+KFSP+ LLDCL++ SEH  LE+A+RIEA+++ WR+K    H K      SSW   V
Sbjct: 321 -TANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKKQAKLKLSSWGGKV 380

Query: 389 KDLMAETDKGDKNTVLAERAESLLLSLKQRFPELSQTTLDTSKIQYNRDVGQAVLESYSR 448
           K L+ + ++ D    L +RAE+LL SL+ RFP L QTTLD +KIQYN+DVGQ++LESYSR
Sbjct: 381 KGLVNDNERND---FLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSR 440

Query: 449 VLEGLAFNIVAWIEDVLFADRSIR 466
           V+E +AFNI A I+DVL+ D ++R
Sbjct: 441 VMESMAFNITARIDDVLYVDDAMR 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875284.11.6e-24492.09rop guanine nucleotide exchange factor 3-like [Benincasa hispida][more]
XP_004138590.18.1e-24491.45rop guanine nucleotide exchange factor 3 [Cucumis sativus] >KGN45658.1 hypotheti... [more]
XP_008458234.11.7e-24190.81PREDICTED: rop guanine nucleotide exchange factor 3 isoform X2 [Cucumis melo] >T... [more]
XP_008458233.11.2e-23989.85PREDICTED: rop guanine nucleotide exchange factor 3 isoform X1 [Cucumis melo][more]
XP_023000613.16.0e-23188.87rop guanine nucleotide exchange factor 3-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A4IJ271.9e-16363.52Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9LQ892.2e-15961.73Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q0WNP73.1e-13756.44Rop guanine nucleotide exchange factor 4 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9LZN02.5e-12355.63Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q93ZY21.1e-11557.55Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Match NameE-valueIdentityDescription
A0A0A0K8493.9e-24491.45PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G004490 PE=4 ... [more]
A0A5D3BTM48.1e-24290.81Rop guanine nucleotide exchange factor 3 isoform X2 OS=Cucumis melo var. makuwa ... [more]
A0A1S3C7D58.1e-24290.81rop guanine nucleotide exchange factor 3 isoform X2 OS=Cucumis melo OX=3656 GN=L... [more]
A0A1S3C7I25.8e-24089.85rop guanine nucleotide exchange factor 3 isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
A0A6J1KN412.9e-23188.87rop guanine nucleotide exchange factor 3-like isoform X2 OS=Cucurbita maxima OX=... [more]
Match NameE-valueIdentityDescription
AT4G00460.21.4e-16463.52RHO guanyl-nucleotide exchange factor 3 [more]
AT1G01700.11.6e-16061.73RHO guanyl-nucleotide exchange factor 2 [more]
AT2G45890.12.2e-13856.44RHO guanyl-nucleotide exchange factor 4 [more]
AT5G02010.11.8e-12455.63RHO guanyl-nucleotide exchange factor 7 [more]
AT4G38430.18.1e-11757.55rho guanyl-nucleotide exchange factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 336..468
e-value: 9.3E-18
score: 66.3
coord: 91..335
e-value: 4.2E-102
score: 342.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..39
NoneNo IPR availablePANTHERPTHR33101:SF47ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2-RELATEDcoord: 20..466
IPR005512PRONE domainPFAMPF03759PRONEcoord: 99..465
e-value: 2.4E-176
score: 585.9
IPR005512PRONE domainPROSITEPS51334PRONEcoord: 91..468
score: 111.940071
IPR038937Rop guanine nucleotide exchange factorPANTHERPTHR33101ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 20..466

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016103.1Lag0016103.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity