Lag0016096 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016096
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionF-box domain-containing protein
Locationchr12: 33088652 .. 33089298 (+)
RNA-Seq ExpressionLag0016096
SyntenyLag0016096
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGTTGGAGATGACTAGGAGGAGGAGGAAGATTATGAAGGGCAATTGGGAGCTTCTGCCTCATATTCATGACCTAATTTTCTCCAAACTCTATATCTCCAATTTTCCTGTCTGCAGGCTTGTCTGTAAATCTTGGAATGGTCTAGTTTTAGATTACGCTTCTTTTTCTTCTAAATTACTCCTTTCAAACATTCTCCTTTTCTCTGATTTCATTCCTCCGAGATGTAGTCTTAGTCGTTGGAAGACCAAGATCCATTGTCTCGATATTGATAAGCTGAGTTTGACGACACTGATGGGTCAGAGATCATCTTTCTCAACTCCTGCAATGGCCTGCTCTTTATTTCCAAACATTTCTCCTGTGACTATTTCAAAGATGTCTCCTCTTCGAAAGATTCTCCATGTGAGTGTTTCTATGATGATGACATATTTAATCCAATGACTGGGGAATTCTTCAAACTTAATGGACCTGTATCAGAAGATGATAATTTCGTATTCGGATTTGGTTTCAACCCTAATACCAAGCAGTACAAATTGTTCAAAGCAAACTACCTAGAATCCAGTCTTACAATGGAGATTTTCACGATTGGTAGAGGTGGTGTTGGGAATTGGGTGTGCAACCAAAGTCCCACATTGGCTAGATAA

mRNA sequence

ATGGAGAAGTTGGAGATGACTAGGAGGAGGAGGAAGATTATGAAGGGCAATTGGGAGCTTCTGCCTCATATTCATGACCTAATTTTCTCCAAACTCTATATCTCCAATTTTCCTGTCTGCAGGCTTGTCTGTAAATCTTGGAATGGTCTAGTTTTAGATTACGCTTCTTTTTCTTCTAAATTACTCCTTTCAAACATTCTCCTTTTCTCTGATTTCATTCCTCCGAGATGTAGTCTTAGTCGTTGGAAGACCAAGATCCATTGTCTCGATATTGATAAGCTGAGTTTGACGACACTGATGGATGTCTCCTCTTCGAAAGATTCTCCATGTGAGTGTTTCTATGATGATGACATATTTAATCCAATGACTGGGGAATTCTTCAAACTTAATGGACCTGTATCAGAAGATGATAATTTCGTATTCGGATTTGGTTTCAACCCTAATACCAAGCAGTACAAATTGTTCAAAGCAAACTACCTAGAATCCAGTCTTACAATGGAGATTTTCACGATTGGTAGAGGTGGTGTTGGGAATTGGGTGTGCAACCAAAGTCCCACATTGGCTAGATAA

Coding sequence (CDS)

ATGGAGAAGTTGGAGATGACTAGGAGGAGGAGGAAGATTATGAAGGGCAATTGGGAGCTTCTGCCTCATATTCATGACCTAATTTTCTCCAAACTCTATATCTCCAATTTTCCTGTCTGCAGGCTTGTCTGTAAATCTTGGAATGGTCTAGTTTTAGATTACGCTTCTTTTTCTTCTAAATTACTCCTTTCAAACATTCTCCTTTTCTCTGATTTCATTCCTCCGAGATGTAGTCTTAGTCGTTGGAAGACCAAGATCCATTGTCTCGATATTGATAAGCTGAGTTTGACGACACTGATGGATGTCTCCTCTTCGAAAGATTCTCCATGTGAGTGTTTCTATGATGATGACATATTTAATCCAATGACTGGGGAATTCTTCAAACTTAATGGACCTGTATCAGAAGATGATAATTTCGTATTCGGATTTGGTTTCAACCCTAATACCAAGCAGTACAAATTGTTCAAAGCAAACTACCTAGAATCCAGTCTTACAATGGAGATTTTCACGATTGGTAGAGGTGGTGTTGGGAATTGGGTGTGCAACCAAAGTCCCACATTGGCTAGATAA

Protein sequence

MEKLEMTRRRRKIMKGNWELLPHIHDLIFSKLYISNFPVCRLVCKSWNGLVLDYASFSSKLLLSNILLFSDFIPPRCSLSRWKTKIHCLDIDKLSLTTLMDVSSSKDSPCECFYDDDIFNPMTGEFFKLNGPVSEDDNFVFGFGFNPNTKQYKLFKANYLESSLTMEIFTIGRGGVGNWVCNQSPTLAR
Homology
BLAST of Lag0016096 vs. NCBI nr
Match: XP_038879760.1 (uncharacterized protein LOC120071509 [Benincasa hispida])

HSP 1 Score: 114.0 bits (284), Expect = 1.4e-21
Identity = 71/197 (36.04%), Postives = 101/197 (51.27%), Query Frame = 0

Query: 12  KIMKGNWELLPHIHDLIFSKLYISNFPVCRLVCKSWNGLVLDYASFSSKLLLSNILLFSD 71
           KI+    EL   I + IF +L +SN   CR+VCK+WN L+LDY+S SSKLL ++ +LF+ 
Sbjct: 2   KIVNWGRELQEDIGESIFGRLVVSNLATCRVVCKTWNSLILDYSS-SSKLLTNDFILFTR 61

Query: 72  FIPPRC-SLSRWKTKIHCLDIDKLSLTTLMDVSSSKDSP--------------------- 131
              P C S S +  K+HC  +D L + + M+   +K +                      
Sbjct: 62  DGNPICGSSSLYNPKMHCFRLDDLDVDSDMEFEVNKFASLRFDGDWSFILPIRSCNGLLL 121

Query: 132 -------CECFYDDDIFNPMTGEFFKLNGPVSEDDNFVFGFGFNPNTKQYKLFKANYLES 177
                  C C     IFNPMT EFF++     + + +V+GFGF P TK YKLF+    + 
Sbjct: 122 ISKSTFYCSCM---GIFNPMTNEFFRVPRDNFDANKYVYGFGFIPTTKHYKLFRITIEDE 181

BLAST of Lag0016096 vs. NCBI nr
Match: XP_008459707.1 (PREDICTED: F-box protein At3g07870-like [Cucumis melo])

HSP 1 Score: 110.5 bits (275), Expect = 1.6e-20
Identity = 79/201 (39.30%), Postives = 103/201 (51.24%), Query Frame = 0

Query: 1   MEKLEMTRRRRKIMKGNWELLPHIHDLIFSKLYISNFPVCRLVCKSWNGLVLDYASFSSK 60
           ME LE   ++R  M  N  L PHI   IFSKL ISN P CR+VCK+WN LVL+YAS +  
Sbjct: 1   MEILE--TKKRIPMNINLRLPPHIVRAIFSKLSISNLPTCRVVCKTWNNLVLEYASSTKF 60

Query: 61  LLLSNILL-----FSDFIPPRCSLSRWKTKIHCLDIDK-------------LSLTTLMDV 120
           L  + + L      S++IP + S      KIHC+DID               S  T+++ 
Sbjct: 61  LPTTTLFLSMADEISNYIPFK-STPWCNPKIHCVDIDSKTSYVASFGFEGDWSYITIVNS 120

Query: 121 SSSKDSPCECFYD---DDIFNPMTGEFFKLNGPVSEDDNFVFGFGFNPNTKQYKLFKA-- 175
            +     C+  +D   + I NPMT EF ++  P  E D    GFGF+P TKQYKLF+   
Sbjct: 121 CNGLLYICKFTFDSLCEGILNPMTNEFMEIPQPEIELDVHSIGFGFSPRTKQYKLFRTEP 180

BLAST of Lag0016096 vs. NCBI nr
Match: KAE8653057.1 (hypothetical protein Csa_020016 [Cucumis sativus])

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-17
Identity = 71/219 (32.42%), Postives = 103/219 (47.03%), Query Frame = 0

Query: 1   MEKLEMTRRRRKIMKGNWELLPHIHDLIFSKLYISNFPVCRLVCKSWNGLVLDYASFSSK 60
           +E +E++ RR  I  G   L P I   IFSKL IS+ P CRLV  +WN LVLDYAS +  
Sbjct: 8   IENMEISERRMTINCGK-GLPPLIAKTIFSKLVISDLPSCRLVSNTWNDLVLDYASSTQL 67

Query: 61  LLLSNILLFSDFIPPRCSLSRWKTKIHCLDIDKLSLTTLMDVSSSKD------------- 120
             L N  L S       +      K+HC+++D   L+   D     +             
Sbjct: 68  QFLKNAFLLST-SDRGLNYKLCNPKMHCINLDTRQLSNDFDFDLESEFIKSASLQFDGDW 127

Query: 121 ----------------SPCECF-YDDDIFNPMTGEFFKLNGPVSEDDNFVFGFGFNPNTK 179
                           S C  + +   IFNPMT EF +++   + DD +++GFGF+P TK
Sbjct: 128 TYTIIMAGSCNGLMLISKCSDYTWCQGIFNPMTNEFLQVSEHGTFDDFYLYGFGFSPITK 187

BLAST of Lag0016096 vs. NCBI nr
Match: XP_011648473.2 (F-box protein At3g07870 [Cucumis sativus])

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-17
Identity = 71/219 (32.42%), Postives = 103/219 (47.03%), Query Frame = 0

Query: 1   MEKLEMTRRRRKIMKGNWELLPHIHDLIFSKLYISNFPVCRLVCKSWNGLVLDYASFSSK 60
           +E +E++ RR  I  G   L P I   IFSKL IS+ P CRLV  +WN LVLDYAS +  
Sbjct: 8   IENMEISERRMTINCGK-GLPPLIAKTIFSKLVISDLPSCRLVSNTWNDLVLDYASSTQL 67

Query: 61  LLLSNILLFSDFIPPRCSLSRWKTKIHCLDIDKLSLTTLMDVSSSKD------------- 120
             L N  L S       +      K+HC+++D   L+   D     +             
Sbjct: 68  QFLKNAFLLST-SDRGLNYKLCNPKMHCINLDTRQLSNDFDFDLESEFIKSASLQFDGDW 127

Query: 121 ----------------SPCECF-YDDDIFNPMTGEFFKLNGPVSEDDNFVFGFGFNPNTK 179
                           S C  + +   IFNPMT EF +++   + DD +++GFGF+P TK
Sbjct: 128 TYTIIMAGSCNGLMLISKCSDYTWCQGIFNPMTNEFLQVSEHGTFDDFYLYGFGFSPITK 187

BLAST of Lag0016096 vs. NCBI nr
Match: XP_011660339.1 (putative F-box protein At1g32420 [Cucumis sativus])

HSP 1 Score: 100.1 bits (248), Expect = 2.1e-17
Identity = 64/163 (39.26%), Postives = 87/163 (53.37%), Query Frame = 0

Query: 28  IFSKLYISNFPVCRLVCKSWNGLVLDYASFSSKLLLSNILLFS-DFIPP-RCSLSRWKTK 87
           IFSKL +SN P CR+VCK+WN L+LD+AS +SK L ++ +LF+ D + P  C  +     
Sbjct: 18  IFSKLLVSNLPACRVVCKAWNELILDHASSASKFLTNDFILFTCDVLHPFPCPDNHTNPN 77

Query: 88  IHCLDIDKLSLTTLMDV------SSSKDS-----------------------PCECFYDD 147
           +HCL  D L L   +D+      SSS ++                       P E     
Sbjct: 78  MHCLRFDNLDLDLDLDLELEVNKSSSFEAGVVYKLINSCNGLLLIYKLPLCFPGEFLLRV 137

Query: 148 DIFNPMTGEFFKLNGPVSEDDNFVFGFGFNPNTKQYKLFKANY 160
            IFNPMT EFF++  P  E   + +GFGF P TKQYKLF+ N+
Sbjct: 138 GIFNPMTNEFFQV--PHDEIVEYHYGFGFIPATKQYKLFRVNF 178

BLAST of Lag0016096 vs. ExPASy Swiss-Prot
Match: Q9SFC7 (F-box protein At3g07870 OS=Arabidopsis thaliana OX=3702 GN=At3g07870 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.6e-04
Identity = 51/166 (30.72%), Postives = 71/166 (42.77%), Query Frame = 0

Query: 16  GNWELLPH-IHDLIFSKLYISNFPVCRLVCKSWNGLVLDY---ASFSSKLLLSNILLFSD 75
           G  E LP  I   IFS+L IS+      VC+SW  ++  +   +S SS      +LL  D
Sbjct: 23  GGLESLPEDIIADIFSRLPISSIARLMFVCRSWRSVLTQHGRLSSSSSSPTKPCLLLHCD 82

Query: 76  --------FIPPRCSLSRWKTKIHCLDIDKLSLTTLMDVSSSKDSPC--ECFYDDD--IF 135
                   F+       R KTK   L     S+     V S     C  +  Y+D   ++
Sbjct: 83  SPIRNGLHFLDLSEEEKRIKTKKFTLRFAS-SMPEFDVVGSCNGLLCLSDSLYNDSLYLY 142

Query: 136 NPMTGEFFKLNGPVSE--DDNFVFGFGFNPNTKQYKLFKANYLESS 164
           NP T    +L    ++  D   VFGFGF+  TK+YK+ K  Y   S
Sbjct: 143 NPFTTNSLELPECSNKYHDQELVFGFGFHEMTKEYKVLKIVYFRGS 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879760.11.4e-2136.04uncharacterized protein LOC120071509 [Benincasa hispida][more]
XP_008459707.11.6e-2039.30PREDICTED: F-box protein At3g07870-like [Cucumis melo][more]
KAE8653057.11.6e-1732.42hypothetical protein Csa_020016 [Cucumis sativus][more]
XP_011648473.21.6e-1732.42F-box protein At3g07870 [Cucumis sativus][more]
XP_011660339.12.1e-1739.26putative F-box protein At1g32420 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9SFC73.6e-0430.72F-box protein At3g07870 OS=Arabidopsis thaliana OX=3702 GN=At3g07870 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR47993OS09G0372900 PROTEIN-RELATEDcoord: 23..179
NoneNo IPR availablePANTHERPTHR47993:SF20F-BOX FAMILY PROTEINcoord: 23..179
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 22..83

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016096.1Lag0016096.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding