Lag0016086 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016086
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr12: 32972396 .. 32973676 (-)
RNA-Seq ExpressionLag0016086
SyntenyLag0016086
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCCCCCACTCAAAGTGCCTTTGTTCCAGGACGGAATATTTGTGATAATGTTATTCTAGGGTTTGAGTGCATGCATTATCTAAAGCAGAAGAGAAAGGGGAAGACGGGGTGGGCGGCCTTGAAACTCGACATGAGCAAGGCTTATGATCGGGTAGAGTGGTTCTTTTTAGAGAAGTTCATGGGGGCACTGGGCATTGATGGGAGAGTGGTGGAGCTTATCATGAGGTGTGTGAGTACTGTCTCATATTCTTTTAGATTGAATGGCCAGGTGGTGGGGAGGATTGTCCCTTCTAGGGGCCTCCGCCAAAGGGACCCCATTTCTCCTTACCTATTCTTATTGTGTGTTGAAGGGTTGTCGAGGATGTTGAATTGGTTGGAGGAGGATAAGAGGATGACGGGGGTAAGGATTGCCAGGGGTTGCCCTTCCATATCCCACTTATTTTTTGCAGATGACTGTTTGTTGTTCTTTAAGGCGAATATAAGGGAAGCGCAAATGGTGGCTAAGGTGTTGGAGACCTATGCTATTATTACTGGTCAAGAGATTAATTACAAGAAGTATGGGCTTTGCCTTAGCCCAAACGTGGATGAGCAAGTAGGGGAGGCGATTGCTGCTGTTTTGAATGTTCAGTTAGTTGAGTCTCATGACAAGTATTTGGGATTGCCTGCAGGGTTTGGGGGTAGTAAGGCAGTAGTCCTGAAACAAATAAAGGATAAGATCTGGTTTTACATCCAGAGGTGGAGGCATACGTGTTTTTCGGTGGGTGGGAAGGAGGTTCTTATAAAGGTTGTGCTGCAAGCATTCCCGACCTATTCAATGTCTTTTTTTCGCCTACCGAAAAGTCTAGTTCATGATTGTAACCGAATGATGGCCAGATTCTGGTGGGGAGAGGATGATGGGTTTAGGAGGATACACTGGTTGTCGTGGAAGAAGATGTGTATGTCTAAGTTTGAGGGTGGGCTAGGCTTTCGGGACTTGGAGCTGTTTAATAAGGCTCTCCTAGCAAAACAGAGGTGGAGGCTTATTAATGACCCCGACTCTCTGATTGGAAAGGTCTTGAAGGGGAAGTACTACCGTGATTGCTCCTTCCTGAAAGCGAAGGATAAGGGGAGTGCCTCCTATGTGTGGAAGAGTTTGATGTGGAGGAGGAGTTTGCTTGCTGAGGGGCTTAGATGGAGGGTGGGGGATGGGAGGTCAATAAGGGTTGTGGGAGATAGGTGGATTCCTCGACCCCCTACTCTGAAGCTTATTCACAATGGGGGGTGTGCCCGGAGATGA

mRNA sequence

ATGATTTCCCCCACTCAAAGTGCCTTTGTTCCAGGACGGAATATTTGTGATAATGTTATTCTAGGGTTTGAGTGCATGCATTATCTAAAGCAGAAGAGAAAGGGGAAGACGGGGTGGGCGGCCTTGAAACTCGACATGAGCAAGGCTTATGATCGGGTAGAGTGGTTCTTTTTAGAGAAGTTCATGGGGGCACTGGGCATTGATGGGAGAGTGGTGGAGCTTATCATGAGGTGTGTGAGTACTGTCTCATATTCTTTTAGATTGAATGGCCAGGTGGTGGGGAGGATTGTCCCTTCTAGGGGCCTCCGCCAAAGGGACCCCATTTCTCCTTACCTATTCTTATTGTGTGTTGAAGGGTTGTCGAGGATGTTGAATTGGTTGGAGGAGGATAAGAGGATGACGGGGGTAAGGATTGCCAGGGGTTGCCCTTCCATATCCCACTTATTTTTTGCAGATGACTGTTTGTTGTTCTTTAAGGCGAATATAAGGGAAGCGCAAATGGTGGCTAAGGTGTTGGAGACCTATGCTATTATTACTGGTCAAGAGATTAATTACAAGAAGTATGGGCTTTGCCTTAGCCCAAACGTGGATGAGCAAGTAGGGGAGGCGATTGCTGCTGTTTTGAATGTTCAGTTAGTTGAGTCTCATGACAAGTATTTGGGATTGCCTGCAGGGTTTGGGGGTAGTAAGGCAGTAGTCCTGAAACAAATAAAGGATAAGATCTGGTTTTACATCCAGAGGTGGAGGCATACGTGTTTTTCGGTGGGTGGGAAGGAGGTTCTTATAAAGGTTGTGCTGCAAGCATTCCCGACCTATTCAATGTCTTTTTTTCGCCTACCGAAAAGTCTAGTTCATGATTGTAACCGAATGATGGCCAGATTCTGGTGGGGAGAGGATGATGGGTTTAGGAGGATACACTGGTTGTCGTGGAAGAAGATGTGTATGTCTAAGTTTGAGGGTGGGCTAGGCTTTCGGGACTTGGAGCTGTTTAATAAGGCTCTCCTAGCAAAACAGAGGTGGAGGCTTATTAATGACCCCGACTCTCTGATTGGAAAGGTCTTGAAGGGGAAGTACTACCGTGATTGCTCCTTCCTGAAAGCGAAGGATAAGGGGAGTGCCTCCTATGTGTGGAAGAGTTTGATGTGGAGGAGGAGTTTGCTTGCTGAGGGGCTTAGATGGAGGGTGGGGGATGGGAGGTCAATAAGGGTTGTGGGAGATAGGTGGATTCCTCGACCCCCTACTCTGAAGCTTATTCACAATGGGGGGTGTGCCCGGAGATGA

Coding sequence (CDS)

ATGATTTCCCCCACTCAAAGTGCCTTTGTTCCAGGACGGAATATTTGTGATAATGTTATTCTAGGGTTTGAGTGCATGCATTATCTAAAGCAGAAGAGAAAGGGGAAGACGGGGTGGGCGGCCTTGAAACTCGACATGAGCAAGGCTTATGATCGGGTAGAGTGGTTCTTTTTAGAGAAGTTCATGGGGGCACTGGGCATTGATGGGAGAGTGGTGGAGCTTATCATGAGGTGTGTGAGTACTGTCTCATATTCTTTTAGATTGAATGGCCAGGTGGTGGGGAGGATTGTCCCTTCTAGGGGCCTCCGCCAAAGGGACCCCATTTCTCCTTACCTATTCTTATTGTGTGTTGAAGGGTTGTCGAGGATGTTGAATTGGTTGGAGGAGGATAAGAGGATGACGGGGGTAAGGATTGCCAGGGGTTGCCCTTCCATATCCCACTTATTTTTTGCAGATGACTGTTTGTTGTTCTTTAAGGCGAATATAAGGGAAGCGCAAATGGTGGCTAAGGTGTTGGAGACCTATGCTATTATTACTGGTCAAGAGATTAATTACAAGAAGTATGGGCTTTGCCTTAGCCCAAACGTGGATGAGCAAGTAGGGGAGGCGATTGCTGCTGTTTTGAATGTTCAGTTAGTTGAGTCTCATGACAAGTATTTGGGATTGCCTGCAGGGTTTGGGGGTAGTAAGGCAGTAGTCCTGAAACAAATAAAGGATAAGATCTGGTTTTACATCCAGAGGTGGAGGCATACGTGTTTTTCGGTGGGTGGGAAGGAGGTTCTTATAAAGGTTGTGCTGCAAGCATTCCCGACCTATTCAATGTCTTTTTTTCGCCTACCGAAAAGTCTAGTTCATGATTGTAACCGAATGATGGCCAGATTCTGGTGGGGAGAGGATGATGGGTTTAGGAGGATACACTGGTTGTCGTGGAAGAAGATGTGTATGTCTAAGTTTGAGGGTGGGCTAGGCTTTCGGGACTTGGAGCTGTTTAATAAGGCTCTCCTAGCAAAACAGAGGTGGAGGCTTATTAATGACCCCGACTCTCTGATTGGAAAGGTCTTGAAGGGGAAGTACTACCGTGATTGCTCCTTCCTGAAAGCGAAGGATAAGGGGAGTGCCTCCTATGTGTGGAAGAGTTTGATGTGGAGGAGGAGTTTGCTTGCTGAGGGGCTTAGATGGAGGGTGGGGGATGGGAGGTCAATAAGGGTTGTGGGAGATAGGTGGATTCCTCGACCCCCTACTCTGAAGCTTATTCACAATGGGGGGTGTGCCCGGAGATGA

Protein sequence

MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEKFMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGLSRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITGQEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDKIWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDDGFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYRDCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRPPTLKLIHNGGCARR
Homology
BLAST of Lag0016086 vs. NCBI nr
Match: CAB4268750.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 458.4 bits (1178), Expect = 6.9e-125
Identity = 212/418 (50.72%), Postives = 283/418 (67.70%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +IS +QSAFVPGR I DN I+ FE +H++ ++  G+ G+ ALKLDMSKAYDRVEW FLE 
Sbjct: 469 LISDSQSAFVPGRMISDNSIVAFELLHFMHKRTHGRQGYMALKLDMSKAYDRVEWNFLES 528

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            M  +G D R V+LIM C+++VSYSF LNG  VG ++P RGLRQ DP+SPYLFLLC E  
Sbjct: 529 LMLVMGFDRRWVQLIMACLTSVSYSFLLNGNPVGYVIPQRGLRQGDPLSPYLFLLCAEAF 588

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           S ++   E++  + GV + RG P++SHLFFADD  LF KA+      + ++L+ Y  ++G
Sbjct: 589 SGLIVQAEQNGTLHGVSLCRGAPTVSHLFFADDSFLFLKASQSGCAQLKQILQCYEQVSG 648

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
           Q+IN  K  +  S NV ++  E  A  L V+ V  HD+YLGLP   G S+      +KD 
Sbjct: 649 QKINLDKSCVSFSRNVADEDQELFATTLRVRRVAQHDRYLGLPTHIGRSRGQCFNYLKDC 708

Query: 241 IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
           IW  IQ W+    S  GKEVL+KVV QA P Y MS F +PK L  +  +MMA +WWG  +
Sbjct: 709 IWKKIQGWKAKLLSFAGKEVLLKVVAQALPIYMMSCFLIPKFLCEEIQQMMASYWWGAQE 768

Query: 301 GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
           G R+IHWLSWK +C  K EGGLGFR+L  FN AL+AKQ WRL ++P SL+ ++LK +YYR
Sbjct: 769 GRRKIHWLSWKNLCRPKHEGGLGFRNLYAFNLALVAKQVWRLAHNPHSLVARLLKARYYR 828

Query: 361 DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRPPTLKLI 419
           DCS L+A    S SYVW+SL   + +L  G RWR+GDG+++R+  DRW+P P + K++
Sbjct: 829 DCSILEATLGNSPSYVWRSLCQAKVVLERGSRWRIGDGQTVRIWEDRWLPTPVSFKVL 886

BLAST of Lag0016086 vs. NCBI nr
Match: XP_024172304.2 (uncharacterized protein LOC112178381 [Rosa chinensis])

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-123
Identity = 217/412 (52.67%), Postives = 282/412 (68.45%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +I+PTQSAFVPGR I DN +L FE  H+LK++  G  G+ ALKLDMSKAYDRVEW F+E 
Sbjct: 549 IIAPTQSAFVPGRQISDNSLLAFELSHFLKRRTGGSHGYGALKLDMSKAYDRVEWEFIEA 608

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            M ++G D   ++ IM CV+TVSYSF LNG+  G ++P+RGLRQ D ISPYLFLLC EGL
Sbjct: 609 VMRSMGFDQIWIKWIMGCVTTVSYSFLLNGEPRGHLIPTRGLRQGDSISPYLFLLCAEGL 668

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           SRML++ EE  R+ G+ IA G PSI+HLFFADD  +F KA   E   V ++L+ Y   +G
Sbjct: 669 SRMLSYEEEQHRLHGIAIAMGAPSINHLFFADDSFVFMKAEREECARVKEILKWYEDASG 728

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
           Q++N++K  +  S NVD    E +A V  V+ V+ HDKYLGLP     SK    + I +K
Sbjct: 729 QQVNFQKSKISFSKNVDIGCQEELAEVFGVERVDKHDKYLGLPTEVSYSKTEAFQFIMEK 788

Query: 241 IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
               ++ W+    SV GKEV+IK V+Q+ PTY MS F LPK L  + +R MA FWWG+ +
Sbjct: 789 TRNKMKNWKDKTLSVAGKEVMIKSVVQSVPTYVMSCFELPKHLCQEMHRCMAEFWWGDSE 848

Query: 301 GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
             R+IHWL+W KMC+ K +GGLGFR++E FN+ALLAKQ WR++  PDSL+GK LK KY+ 
Sbjct: 849 KGRKIHWLAWDKMCVPKEKGGLGFRNMEYFNQALLAKQGWRILRHPDSLLGKTLKAKYFP 908

Query: 361 DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRP 413
           +  F+ A      SY W+SLM  + LL +GLR++VG G  I V  D WIPRP
Sbjct: 909 NNDFIHASVNQGDSYTWRSLMKGKVLLEKGLRFQVGSGTRISVWFDPWIPRP 960

BLAST of Lag0016086 vs. NCBI nr
Match: PRQ56718.1 (putative RNA-directed DNA polymerase [Rosa chinensis])

HSP 1 Score: 453.4 bits (1165), Expect = 2.2e-123
Identity = 217/412 (52.67%), Postives = 280/412 (67.96%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +ISP QSAFVPGR I DN ++ FE  H LK++R GK G+ ALKLDMSKAYDRVEW FLE 
Sbjct: 551 LISPFQSAFVPGRLISDNSLMAFEVSHCLKRRRSGKVGYCALKLDMSKAYDRVEWVFLEA 610

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            M  +G     +  IM CVSTVSYSF +NG+  GR++PSRGLRQ D ISPYLFLLC E L
Sbjct: 611 VMRKMGFGEEWIRWIMDCVSTVSYSFIINGEPRGRVIPSRGLRQGDSISPYLFLLCSEVL 670

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           SR +   E    + GVRI  G PSISHLFFADD  +FF+A   + ++V  +L  Y   +G
Sbjct: 671 SRSILNAERIGSLYGVRICMGAPSISHLFFADDSFIFFRAGRDDCEVVKSLLLAYERASG 730

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
           Q++NY+K  +  S NVD  + E IA +L V  V+ HDKYLGLP     SK      + +K
Sbjct: 731 QQVNYQKSCISFSKNVDILMQEGIATILGVIRVDKHDKYLGLPIELSYSKVEAFGFLNEK 790

Query: 241 IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
           I    Q WR    S  GKEVLIK V QA P+Y MS F +P+ L  + +R++A+FWWG++ 
Sbjct: 791 IRNRTQGWREKTLSTAGKEVLIKAVAQAIPSYVMSCFEVPRYLCDEMHRVIAQFWWGDEG 850

Query: 301 GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
             R+IHW++W+K+C  K +GGLGFR++ +FN ALL KQ WRLI  PDS+I K+LK KY+ 
Sbjct: 851 DARKIHWIAWEKLCYPKCQGGLGFRNMHIFNLALLTKQGWRLIQQPDSIIAKLLKAKYFP 910

Query: 361 DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRP 413
            CSFL+A+ KG  SY W+S++  R +L +G+R++VGDG SIRV  D W+P P
Sbjct: 911 RCSFLEAELKGGESYTWRSILLGRKVLEKGMRFQVGDGESIRVWDDPWVPVP 962

BLAST of Lag0016086 vs. NCBI nr
Match: XP_024156142.1 (uncharacterized protein LOC112164137 [Rosa chinensis])

HSP 1 Score: 453.0 bits (1164), Expect = 2.9e-123
Identity = 218/412 (52.91%), Postives = 281/412 (68.20%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +I+PTQSAFVPGR I DN +L FE  H+LK++  G  G+ ALKLDMSKAYDRVEW F+E 
Sbjct: 341 IIAPTQSAFVPGRQISDNSLLAFELSHFLKRRTGGSHGYGALKLDMSKAYDRVEWEFIEA 400

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            M ++G D   +  IM CV+TVSYSF LNG+  G ++P+RGLRQ D ISPYLFLLC EGL
Sbjct: 401 VMRSMGFDQIWINWIMGCVTTVSYSFLLNGEPRGHLIPTRGLRQGDSISPYLFLLCAEGL 460

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           SRML++ EE  R+ G+ IA G PSI+HLFFADD  +F KA   E   V ++L+ Y   +G
Sbjct: 461 SRMLSYEEEQHRLHGIAIAMGAPSINHLFFADDSFVFMKAEREECARVKEILKWYEDASG 520

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
           Q++N++K  +  S NVD    E +A V  V+ V+ HDKYLGLP     SK    + I +K
Sbjct: 521 QQVNFQKSKISFSKNVDIGCQEELAEVFGVERVDKHDKYLGLPTKVSYSKIEAFQFIMEK 580

Query: 241 IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
               ++ W+    SV GKEV+IK V+Q+ PTY MS F LPK L  + +R MA FWWG+ +
Sbjct: 581 TKNKMKNWKDKTLSVAGKEVMIKSVVQSVPTYVMSCFELPKHLCQEMHRCMAEFWWGDSE 640

Query: 301 GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
             R+IHWL+W KMC+ K EGGLGFR++E FN+ALLAKQ WR++  PDSL+GK LK KY+ 
Sbjct: 641 KGRKIHWLAWDKMCVPKEEGGLGFRNMEYFNQALLAKQGWRILRHPDSLLGKTLKAKYFP 700

Query: 361 DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRP 413
           +  F+ A      SY W+SLM  + LL +GLR++VG G  I V  D WIPRP
Sbjct: 701 NNDFIHASVNQGDSYTWRSLMKGKVLLEKGLRFQVGLGTRISVWFDPWIPRP 752

BLAST of Lag0016086 vs. NCBI nr
Match: XP_010682492.1 (PREDICTED: uncharacterized protein LOC104897331 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-120
Identity = 207/417 (49.64%), Postives = 278/417 (66.67%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +ISP QSAFVPGR I DN ++ +E  HY+K+    KTG  A KLDMSKAYDRVEW FLE+
Sbjct: 384 LISPYQSAFVPGRLITDNAMIAYEIFHYMKRSGDSKTGSMAFKLDMSKAYDRVEWSFLEQ 443

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            M  +G     V  IM C+S+VSY+F+LNG+V G I+PSRGLRQ DP+SPYLFLLC E  
Sbjct: 444 VMRKMGFCDSWVRRIMVCLSSVSYAFKLNGKVTGNIIPSRGLRQGDPLSPYLFLLCAEAF 503

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           S +L    +D R+ G R+ R  P ISHLFFADD +LF +A ++E  +VA ++  Y   +G
Sbjct: 504 STLLAKASDDGRIHGARVCRSAPRISHLFFADDSILFTRATLQECSVVADIISVYERASG 563

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
           Q+IN+ K  +  S NVD+     I ++L V+ V  HDKYLGLP   G SK  V   +K++
Sbjct: 564 QKINFNKSEVSFSKNVDDSRRVEIRSMLGVREVVKHDKYLGLPTLIGRSKKAVFAVLKER 623

Query: 241 IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
           +W  +Q W+    S  GKEVLIK V+QA PTY MS F +P  ++ D N M ARFWW    
Sbjct: 624 VWKKLQGWKEKLLSKAGKEVLIKAVIQAIPTYMMSLFAIPDGILEDINSMCARFWWRATG 683

Query: 301 GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
             R++HW+SW+K C+ K  GG+GFRDL+ FN+ALLAKQ WRL+ D  SL  ++++ +Y++
Sbjct: 684 MARKMHWISWEKFCLPKSYGGMGFRDLKTFNQALLAKQGWRLMCDDGSLAHQIMRARYFK 743

Query: 361 DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRPPTLKL 418
           +  FL A+     S+VW+S+   +SLL EGL+WRVG+G SIRV    W+P   + K+
Sbjct: 744 NVPFLDARRGYDPSFVWRSIWGAKSLLMEGLKWRVGNGASIRVWDMAWLPGDSSSKV 800

BLAST of Lag0016086 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.6e-30
Identity = 65/151 (43.05%), Postives = 92/151 (60.93%), Query Frame = 0

Query: 268 AFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDDGFRRIHWLSWKKMCMSK-FEGGLGFRD 327
           A P Y+MS FRL K L       M  FWW   +  R+I W++W+K+C SK  +GGLGFRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 328 LELFNKALLAKQRWRLINDPDSLIGKVLKGKYYRDCSFLKAKDKGSASYVWKSLMWRRSL 387
           L  FN+ALLAKQ +R+I+ P +L+ ++L+ +Y+   S ++       SY W+S++  R L
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 388 LAEGLRWRVGDGRSIRVVGDRWI----PRPP 414
           L+ GL   +GDG   +V  DRWI    P PP
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPLPP 152

BLAST of Lag0016086 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.4e-27
Identity = 66/187 (35.29%), Postives = 105/187 (56.15%), Query Frame = 0

Query: 236 QIKDKIWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFW 295
           +I +++   +  WR    S  G+  L K VL + P +SMS   LP+S+++  +++   F 
Sbjct: 15  EILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFL 74

Query: 296 WGEDDGFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLK 355
           WG     ++ H + W K+C  K EGGLG R  +  N+AL++K  WRL+ + +SL   VL+
Sbjct: 75  WGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQ 134

Query: 356 GKYY----RDCSFLKAKDKGSASYVWKSL-MWRRSLLAEGLRWRVGDGRSIRVVGDRWIP 415
            KY+    RD  +L    KGS S  W+S+ +  R +++ G+ W  GDG+ IR   DRW+ 
Sbjct: 135 KKYHVGEIRDSRWL--IPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVS 194

Query: 416 RPPTLKL 418
             P L+L
Sbjct: 195 GKPLLEL 199

BLAST of Lag0016086 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 1.4e-24
Identity = 106/442 (23.98%), Postives = 193/442 (43.67%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +I P Q  F+PG     N+      +HY+  K K K     + LD  KA+D+++  F+ K
Sbjct: 565 IIHPDQVGFIPGMQGWFNIRKSINVIHYI-NKLKDK-NHMIISLDAEKAFDKIQHPFMIK 624

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            +   GI G  + +I    S    + ++NG+ +  I    G RQ  P+SPYLF + +E L
Sbjct: 625 VLERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVL 684

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           +R    + + K + G++I +    IS L  ADD +++        + +  ++ ++  + G
Sbjct: 685 ARA---IRQQKEIKGIQIGKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVG 744

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVV------L 240
            +IN  K  +      ++Q  + I       +V ++ KYLG+      +K V        
Sbjct: 745 YKINSNK-SMAFLYTKNKQAEKEIRETTPFSIVTNNIKYLGVTL----TKEVKDLYDKNF 804

Query: 241 KQIKDKIWFYIQRWRHTCFSVGGKEVLIKVVL--QAFPTYSMSFFRLPKSLVHDCNRMMA 300
           K +K +I   ++RW+    S  G+  ++K+ +  +A   ++    ++P    ++    + 
Sbjct: 805 KSLKKEIKEDLRRWKDLPCSWIGRINIVKMAILPKAIYRFNAIPIKIPTQFFNELEGAIC 864

Query: 301 RFWWGEDDGFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRW-----RLINDPD 360
           +F W           L  K+       GG+   DL+L+ +A++ K  W     R ++  +
Sbjct: 865 KFVWNNKKPRIAKSLLKDKRT-----SGGITMPDLKLYYRAIVIKTAWYWYRDRQVDQWN 924

Query: 361 SLIGKVLKGKYYRDCSFLKAKDKGSASYVWK------SLMWRRSLLAEGLRWRVGDGRS- 419
            +    +    Y    F    DKG+ +  WK      +  W   LL+   R R+    S 
Sbjct: 925 RIEDPEMNPHTYGHLIF----DKGAKTIQWKKDSIFNNWCWHNWLLS-CRRMRIDPYLSP 984

BLAST of Lag0016086 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.6e-23
Identity = 83/348 (23.85%), Postives = 154/348 (44.25%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +I   Q  F+PG     N+      + ++   R        + +D  KA+D+++  F+ K
Sbjct: 558 LIHHDQVGFIPGMQGWFNIRKSINVIQHI--NRAKDKNHVIISIDAEKAFDKIQQPFMLK 617

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            +  LGIDG  +++I       + +  LNGQ +       G RQ  P+SP LF + +E L
Sbjct: 618 TLNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVL 677

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           +R    + ++K + G+++  G   +    FADD +++ +  I  AQ + K++  ++ ++G
Sbjct: 678 ARA---IRQEKEIKGIQL--GKEEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSG 737

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAG------FGGSKAVVL 240
            +IN +K    L  N + Q    I   L   +     KYLG+         F  +   +L
Sbjct: 738 YKINVQKSQAFLYNN-NRQTESQIMGELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLL 797

Query: 241 KQIKDKIWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSF--FRLPKSLVHDCNRMMA 300
           K+IK+       +W++   S  G+  ++K+ +     Y  +    +LP +   +  +   
Sbjct: 798 KEIKED----TNKWKNIPCSWVGRINIVKMAILPKVIYRFNAIPIKLPMTFFTELEKTTL 857

Query: 301 RFWWGEDDGFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRW 341
           +F W +         LS K        GG+   D +L+ KA + K  W
Sbjct: 858 KFIWNQKRARIAKSILSQKNKA-----GGITLPDFKLYYKATVTKTAW 888

BLAST of Lag0016086 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.0e-18
Identity = 82/353 (23.23%), Postives = 154/353 (43.63%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +I   Q  F+PG     N+      + ++  K K K     L +D  KA+D ++  F+ +
Sbjct: 557 IIHHDQVGFIPGSQGWFNIRKSINVIQHI-NKLKNK-DHMILSIDAEKAFDNIQHPFMIR 616

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            +  +GI+G  ++LI    S  + +  LNG  +       G RQ  P+SP LF + +E L
Sbjct: 617 TLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVL 676

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           +     + E+K + G+ I  G   I    FADD +++ +        + +V++ Y+ ++G
Sbjct: 677 AIA---IREEKAIKGIHI--GSEEIKLSLFADDMIVYLENTRDSTTKLLEVIKEYSNVSG 736

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKD- 240
            +IN  K    +  N + Q  + +   +   +V    KYLG+          + K +KD 
Sbjct: 737 YKINTHKSVAFIYTN-NNQAEKTVKDSIPFTVVPKKMKYLGV---------YLTKDVKDL 796

Query: 241 ----------KIWFYIQRWRHTCFSVGGKEVLIK--VVLQAFPTYSMSFFRLPKSLVHDC 300
                     +I   + +W++   S  G+  ++K  ++ +A   ++    + P S   D 
Sbjct: 797 YKENYETLRKEIAEDVNKWKNIPCSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDL 856

Query: 301 NRMMARFWWGEDDGFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRW 341
            +++  F W +         LS K        GG+   DL L+ K+++ K  W
Sbjct: 857 EKIILHFIWNQKKPQIAKTLLSNKNKA-----GGITLPDLRLYYKSIVIKTAW 887

BLAST of Lag0016086 vs. ExPASy TrEMBL
Match: M5W5K8 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa020282mg PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 5.1e-126
Identity = 209/417 (50.12%), Postives = 290/417 (69.54%), Query Frame = 0

Query: 1    MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
            +IS TQSAFVPGR I DN I+ FE +H + +K +G+ G+ ALK+DMSKAYDRVEW FLE 
Sbjct: 895  LISDTQSAFVPGRAISDNSIVAFELLHMMHKKNQGRQGYLALKIDMSKAYDRVEWSFLEA 954

Query: 61   FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
             M  +G   R ++LIM CV+TVSYSF LNG  VG ++P RGLRQ DP+SPYLFLLC E L
Sbjct: 955  LMKGMGFAPRWIQLIMECVTTVSYSFMLNGNPVGYVIPQRGLRQGDPLSPYLFLLCAEAL 1014

Query: 121  SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
            S ++   E    + GV + RG PS+SHLFFADD  LF +A+ ++ + ++ + + Y +++G
Sbjct: 1015 SSLILQAERRNLLHGVNLCRGAPSVSHLFFADDSFLFLRADQQDCEQLSIIFQKYEMVSG 1074

Query: 181  QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
            Q+I+ +K  +  S N+D    + +AAVL V+ V+ HD YLGLP   G S+      +K++
Sbjct: 1075 QKIHLEKSCVSFSINMDRTDQDNLAAVLGVRRVDQHDVYLGLPTHVGRSRRQCFNSLKER 1134

Query: 241  IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
            IW  IQ W+    S  GKE+L+KVV QA P Y M+ F +PK L ++  ++MAR+WW E D
Sbjct: 1135 IWKKIQGWKAKLLSFAGKEILLKVVAQAVPIYMMNCFLIPKCLCNEIQQVMARYWWVEQD 1194

Query: 301  GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
            G R+IHWLSW K+C+ K EGGLGFR+L  FN ALLAKQ WRLI  P+SL+  +LK +Y++
Sbjct: 1195 GQRKIHWLSWNKLCLPKQEGGLGFRNLYAFNMALLAKQLWRLIQTPNSLVACILKARYFK 1254

Query: 361  DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRPPTLKL 418
            +CS L+A+   S SY+W+SL   R L+ +G RWR+G+G S+R+ GDRW+P   + ++
Sbjct: 1255 NCSILEAQIGHSPSYIWQSLCKARVLIEKGSRWRIGNGHSVRIWGDRWLPNSESFQV 1311

BLAST of Lag0016086 vs. ExPASy TrEMBL
Match: A0A6J5TXB8 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS12969 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 3.3e-125
Identity = 212/418 (50.72%), Postives = 283/418 (67.70%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +IS +QSAFVPGR I DN I+ FE +H++ ++  G+ G+ ALKLDMSKAYDRVEW FLE 
Sbjct: 469 LISDSQSAFVPGRMISDNSIVAFELLHFMHKRTHGRQGYMALKLDMSKAYDRVEWNFLES 528

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            M  +G D R V+LIM C+++VSYSF LNG  VG ++P RGLRQ DP+SPYLFLLC E  
Sbjct: 529 LMLVMGFDRRWVQLIMACLTSVSYSFLLNGNPVGYVIPQRGLRQGDPLSPYLFLLCAEAF 588

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           S ++   E++  + GV + RG P++SHLFFADD  LF KA+      + ++L+ Y  ++G
Sbjct: 589 SGLIVQAEQNGTLHGVSLCRGAPTVSHLFFADDSFLFLKASQSGCAQLKQILQCYEQVSG 648

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
           Q+IN  K  +  S NV ++  E  A  L V+ V  HD+YLGLP   G S+      +KD 
Sbjct: 649 QKINLDKSCVSFSRNVADEDQELFATTLRVRRVAQHDRYLGLPTHIGRSRGQCFNYLKDC 708

Query: 241 IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
           IW  IQ W+    S  GKEVL+KVV QA P Y MS F +PK L  +  +MMA +WWG  +
Sbjct: 709 IWKKIQGWKAKLLSFAGKEVLLKVVAQALPIYMMSCFLIPKFLCEEIQQMMASYWWGAQE 768

Query: 301 GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
           G R+IHWLSWK +C  K EGGLGFR+L  FN AL+AKQ WRL ++P SL+ ++LK +YYR
Sbjct: 769 GRRKIHWLSWKNLCRPKHEGGLGFRNLYAFNLALVAKQVWRLAHNPHSLVARLLKARYYR 828

Query: 361 DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRPPTLKLI 419
           DCS L+A    S SYVW+SL   + +L  G RWR+GDG+++R+  DRW+P P + K++
Sbjct: 829 DCSILEATLGNSPSYVWRSLCQAKVVLERGSRWRIGDGQTVRIWEDRWLPTPVSFKVL 886

BLAST of Lag0016086 vs. ExPASy TrEMBL
Match: A0A803Q9W0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 3.7e-124
Identity = 208/417 (49.88%), Postives = 287/417 (68.82%), Query Frame = 0

Query: 1    MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
            +IS  QSAF+ GR I DN ILGFE +H +K+ R G     ALKLDMSKAYDRVEW FLE 
Sbjct: 977  VISENQSAFIRGRLIQDNAILGFESLHCMKKGRFGNGRKMALKLDMSKAYDRVEWRFLET 1036

Query: 61   FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
             M  LG D R V+ IM C+ ++S+S  LNG V G+I PSRGLRQ DP+SPY+FLLC EGL
Sbjct: 1037 MMICLGYDKRWVDKIMNCIKSISFSILLNGDVSGQIHPSRGLRQGDPLSPYMFLLCSEGL 1096

Query: 121  SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
            S ++   E   R+ G+R  R    +SHLFFADD  +F  A   + Q +  +L+ Y++++G
Sbjct: 1097 SCLIQEAERANRLHGLRFGRDNIKLSHLFFADDSFIFLDATPSDCQSLKSILDEYSLLSG 1156

Query: 181  QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
            Q IN+ K  LC+   ++   G  +AA+L V+LV+ H KYLG+PA  G  K  V + I+ K
Sbjct: 1157 QRINFDKSELCVGKQINHGDGILLAAILGVKLVDCHTKYLGIPASVGKKKKEVFEDIRTK 1216

Query: 241  IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
            I   +Q W+ + FS  G+E+L+K ++QA PTY MS FRLPK L+ D + MMARFWWG  D
Sbjct: 1217 IRTKLQGWKASLFSQAGREILLKAIIQAIPTYIMSCFRLPKELIKDIHAMMARFWWGSSD 1276

Query: 301  GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
              ++ HW +WKK+C  K +GG+GF++LELFN++LLAKQ W++IN+P S++ +VLK  YY 
Sbjct: 1277 TKQKTHWGNWKKLCKPKEKGGMGFKNLELFNQSLLAKQGWKIINNPHSMLARVLKACYYT 1336

Query: 361  DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRPPTLKL 418
            + +FL+AK  G  SY+W+S++W R ++ +G+RWRV  GR +R+  D+W+PRP T  L
Sbjct: 1337 NSNFLEAKVGGFGSYMWRSILWGRQIIDKGIRWRVMAGRDVRINEDKWLPRPSTFSL 1393

BLAST of Lag0016086 vs. ExPASy TrEMBL
Match: A0A2P6SDG4 (Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0340391 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 1.1e-123
Identity = 217/412 (52.67%), Postives = 280/412 (67.96%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +ISP QSAFVPGR I DN ++ FE  H LK++R GK G+ ALKLDMSKAYDRVEW FLE 
Sbjct: 551 LISPFQSAFVPGRLISDNSLMAFEVSHCLKRRRSGKVGYCALKLDMSKAYDRVEWVFLEA 610

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGL 120
            M  +G     +  IM CVSTVSYSF +NG+  GR++PSRGLRQ D ISPYLFLLC E L
Sbjct: 611 VMRKMGFGEEWIRWIMDCVSTVSYSFIINGEPRGRVIPSRGLRQGDSISPYLFLLCSEVL 670

Query: 121 SRMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITG 180
           SR +   E    + GVRI  G PSISHLFFADD  +FF+A   + ++V  +L  Y   +G
Sbjct: 671 SRSILNAERIGSLYGVRICMGAPSISHLFFADDSFIFFRAGRDDCEVVKSLLLAYERASG 730

Query: 181 QEINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDK 240
           Q++NY+K  +  S NVD  + E IA +L V  V+ HDKYLGLP     SK      + +K
Sbjct: 731 QQVNYQKSCISFSKNVDILMQEGIATILGVIRVDKHDKYLGLPIELSYSKVEAFGFLNEK 790

Query: 241 IWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDD 300
           I    Q WR    S  GKEVLIK V QA P+Y MS F +P+ L  + +R++A+FWWG++ 
Sbjct: 791 IRNRTQGWREKTLSTAGKEVLIKAVAQAIPSYVMSCFEVPRYLCDEMHRVIAQFWWGDEG 850

Query: 301 GFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYR 360
             R+IHW++W+K+C  K +GGLGFR++ +FN ALL KQ WRLI  PDS+I K+LK KY+ 
Sbjct: 851 DARKIHWIAWEKLCYPKCQGGLGFRNMHIFNLALLTKQGWRLIQQPDSIIAKLLKAKYFP 910

Query: 361 DCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRP 413
            CSFL+A+ KG  SY W+S++  R +L +G+R++VGDG SIRV  D W+P P
Sbjct: 911 RCSFLEAELKGGESYTWRSILLGRKVLEKGMRFQVGDGESIRVWDDPWVPVP 962

BLAST of Lag0016086 vs. ExPASy TrEMBL
Match: A0A803PBM9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 1.4e-120
Identity = 205/411 (49.88%), Postives = 278/411 (67.64%), Query Frame = 0

Query: 2    ISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEKF 61
            IS  QSAFV GR I DN I+GFE +H +K++R G     ALKLDMSKAYDRVEW FL   
Sbjct: 970  ISEEQSAFVGGRLIQDNAIIGFESLHCMKKRRFGNRRKMALKLDMSKAYDRVEWGFLITM 1029

Query: 62   MGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGLS 121
            M  LG +   +E IMRCV++VS+S  +NG+ +G+  P+RGLRQ D +SPYLFL+C EGLS
Sbjct: 1030 MRGLGYEEDWIEKIMRCVTSVSFSVLINGERIGKFTPTRGLRQGDSLSPYLFLICSEGLS 1089

Query: 122  RMLNWLEEDKRMTGVRIARGCPSISHLFFADDCLLFFKANIREAQMVAKVLETYAIITGQ 181
             ++   E    + GVR  +    +SHLFFADD  +F + N  E   ++ +L+ Y+ ++GQ
Sbjct: 1090 CLIQEAERAGNINGVRFGKEGVKVSHLFFADDSFVFLEGNEAECDTMSSILQRYSRLSGQ 1149

Query: 182  EINYKKYGLCLSPNVDEQVGEAIAAVLNVQLVESHDKYLGLPAGFGGSKAVVLKQIKDKI 241
            +IN +K  + +   +  Q+G ++A  L V LV  H KYLGLP+  G  K  V + IKDK+
Sbjct: 1150 QINLEKSEVSMGSRISSQLGNSLANRLGVSLVGQHTKYLGLPSFIGRRKKEVFEMIKDKV 1209

Query: 242  WFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDDG 301
            W  ++ W+ T FS  GKE+LIK V+QA P+YSMS FRLPK L+H  + + A FWWG+   
Sbjct: 1210 WNKLKSWKSTMFSQAGKEILIKAVIQAIPSYSMSCFRLPKKLIHSLHSLAANFWWGDTKE 1269

Query: 302  FRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAKQRWRLINDPDSLIGKVLKGKYYRD 361
             ++IHW +W K+C  K EGGLGFR L  FN+ALLAKQ WRLI+ P SL+ +VLK  YY +
Sbjct: 1270 NKKIHWSTWAKLCKPKEEGGLGFRSLTEFNQALLAKQGWRLIHHPHSLLARVLKHSYYPN 1329

Query: 362  CSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGDGRSIRVVGDRWIPRP 413
             SFL+AK +  AS +W+ + W R ++ EG RWRVG+GR++R+  D+WIPRP
Sbjct: 1330 SSFLQAKCQPGASSIWQGITWGRQIIVEGARWRVGNGRTVRIWEDKWIPRP 1380

BLAST of Lag0016086 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 133.3 bits (334), Expect = 4.7e-31
Identity = 65/151 (43.05%), Postives = 92/151 (60.93%), Query Frame = 0

Query: 268 AFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDDGFRRIHWLSWKKMCMSK-FEGGLGFRD 327
           A P Y+MS FRL K L       M  FWW   +  R+I W++W+K+C SK  +GGLGFRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 328 LELFNKALLAKQRWRLINDPDSLIGKVLKGKYYRDCSFLKAKDKGSASYVWKSLMWRRSL 387
           L  FN+ALLAKQ +R+I+ P +L+ ++L+ +Y+   S ++       SY W+S++  R L
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 388 LAEGLRWRVGDGRSIRVVGDRWI----PRPP 414
           L+ GL   +GDG   +V  DRWI    P PP
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPLPP 152

BLAST of Lag0016086 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 56/146 (38.36%), Postives = 85/146 (58.22%), Query Frame = 0

Query: 268 AFPTYSMSFFRLPKSLVHDCNRMMARFWWGEDDGFRRIHWLSWKKMCMSKFEGGLGFRDL 327
           A PTY+M+ F LPK++      ++A FWW      + +HW +W  +   K EGG+GF+D+
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 328 ELFNKALLAKQRWRLINDPDSLIGKVLKGKYYRDCSFLKAKDKGSASYVWKSLMWRRSLL 387
           E FN ALL KQ WR+++ P+SL+ KV K +Y+     L A      S+VWKS+   + +L
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 388 AEGLRWRVGDGRSIRVVGDRWIPRPP 414
            +G R  VG+G  I +   +W+   P
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWLDSKP 147

BLAST of Lag0016086 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-15
Identity = 35/68 (51.47%), Postives = 46/68 (67.65%), Query Frame = 0

Query: 86  FRLNGQVVGRIVPSRGLRQRDPISPYLFLLCVEGLSRMLNWLEEDKRMTGVRIARGCPSI 145
           F +NG   G + PSRGLRQ DP+SPYLF+LC E LS +    +E  R+ G+R++   P I
Sbjct: 12  FIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRI 71

Query: 146 SHLFFADD 154
           +HL FADD
Sbjct: 72  NHLLFADD 79

BLAST of Lag0016086 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 70.9 bits (172), Expect = 2.9e-12
Identity = 37/106 (34.91%), Postives = 59/106 (55.66%), Query Frame = 0

Query: 1   MISPTQSAFVPGRNICDNVILGFECMHYLKQKRKGKTGWAALKLDMSKAYDRVEWFFLEK 60
           +I P Q++F+PGR   DN++   E +H +++K KG  GW  LKLD+ KAYDR+ W +LE 
Sbjct: 12  LIGPAQASFIPGRVSTDNIVFVQEAVHSMRRK-KGVKGWMLLKLDLEKAYDRIRWDYLED 71

Query: 61  FMGALGIDGRVVELIMRCVSTVSYSFRLNGQVVGRIVPSRGLRQRD 107
            + + G      E+ +  ++  ++  R     VGR   S+  R  D
Sbjct: 72  TLISAGFP----EVWLPEIARSTFGARRVAPEVGRADASKRPRVSD 112

BLAST of Lag0016086 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 67.0 bits (162), Expect = 4.2e-11
Identity = 48/206 (23.30%), Postives = 83/206 (40.29%), Query Frame = 0

Query: 218 KYLGLPAGFGGSKAVVLKQIKDKIWFYIQRWRHTCFSVGGKEVLIKVVLQAFPTYSMSFF 277
           +YLGLP             + +KI   I +W     S  G+  LI  V+ +   + MS F
Sbjct: 26  RYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAF 85

Query: 278 RLPKSLVHDCNRMMARFWWGEDDGFRRIHWLSWKKMCMSKFEGGLGFRDLELFNKALLAK 337
           RLP + + + + + + F W   +   +   ++W  +C  K EGGLG R L+  NK     
Sbjct: 86  RLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEANKGSF-- 145

Query: 338 QRWRLINDPDSLIGKVLKGKYYRDCSFLKAKDKGSASYVWKSLMWRRSLLAEGLRWRVGD 397
             W       S+ G    G                 S++WK ++  R+L +  ++  + +
Sbjct: 146 --W-------SISGNTTLG-----------------SWMWKKILKHRALASGFVKHDIHN 203

Query: 398 GRSIRVVGDRWIPRPPTLKLIHNGGC 424
           G +     D W      + +  + GC
Sbjct: 206 GSNTSFWFDNWSKIGRLIDVTGHRGC 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CAB4268750.16.9e-12550.72unnamed protein product [Prunus armeniaca][more]
XP_024172304.21.7e-12352.67uncharacterized protein LOC112178381 [Rosa chinensis][more]
PRQ56718.12.2e-12352.67putative RNA-directed DNA polymerase [Rosa chinensis][more]
XP_024156142.12.9e-12352.91uncharacterized protein LOC112164137 [Rosa chinensis][more]
XP_010682492.11.8e-12049.64PREDICTED: uncharacterized protein LOC104897331 [Beta vulgaris subsp. vulgaris][more]
Match NameE-valueIdentityDescription
P932956.6e-3043.05Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
P0C2F62.4e-2735.29Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P113691.4e-2423.98LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
O003701.6e-2323.85LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P085482.0e-1823.23LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
M5W5K85.1e-12650.12Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa020282mg PE=4 SV=1[more]
A0A6J5TXB83.3e-12550.72Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A803Q9W03.7e-12449.88Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2P6SDG41.1e-12352.67Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1... [more]
A0A803PBM91.4e-12049.88Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
ATMG00310.14.7e-3143.05RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G29090.11.3e-2838.36Ribonuclease H-like superfamily protein [more]
ATMG01250.11.2e-1551.47RNA-directed DNA polymerase (reverse transcriptase) [more]
AT4G20520.12.9e-1234.91RNA binding;RNA-directed DNA polymerases [more]
AT3G24255.14.2e-1123.30RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 2..209
e-value: 3.6E-27
score: 95.3
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 1..224
score: 9.019111
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 23..414
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 23..414
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 2..223
e-value: 2.29571E-33
score: 122.785
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 5..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016086.1Lag0016086.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016740 transferase activity