Lag0016048 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0016048
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr12: 32398561 .. 32398962 (+)
RNA-Seq Expression	Lag0016048
Synteny	Lag0016048

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTACAAGCTGGCCAAAGCTTTAGCCAATTGCCTCAAAATAGTTTTAAACTCTATTATAGTTCCCACCCAATTCGCTTTTGTCCCGAATAGGTTAATATTGGACAACATGACCATCGGCTTCGAAAGCATTGACACCCTTAATAGCAAAAAAAAAGGGGGAAAAGAAGGTTTTATTGCTATGAAGATCGACATGAGCAAGACTTACGACAGGGTTGAGTGGTTGTACATTGACAAAGTGATGGAGCAAATGGGTTTTAGCGAAAAGTGGAGAAGTAGCATATCTAACTTCTTGGCCTCGGTCCAATTCTCGGTGTTGGTGAATGGAATTCCTAGTGAAAGCTTCAAGCCAAAAAGAGGAATTAGGCAGGGAGATCCGCTCCCCCTTACATTTTCTTGA

mRNA sequence

ATGTCGTACAAGCTGGCCAAAGCTTTAGCCAATTGCCTCAAAATAGTTTTAAACTCTATTATAGTTCCCACCCAATTCGCTTTTGTCCCGAATAGGTTAATATTGGACAACATGACCATCGGCTTCGAAAGCATTGACACCCTTAATAGCAAAAAAAAAGGGGGAAAAGAAGGTTTTATTGCTATGAAGATCGACATGAGCAAGACTTACGACAGGGTTGAGTGGTTGTACATTGACAAAGTGATGGAGCAAATGGGTTTTAGCGAAAAGTGGAGAAGTAGCATATCTAACTTCTTGGCCTCGGTCCAATTCTCGGTGTTGGTGAATGGAATTCCTAGTGAAAGCTTCAAGCCAAAAAGAGGAATTAGGCAGGGAGATCCGCTCCCCCTTACATTTTCTTGA

Coding sequence (CDS)

ATGTCGTACAAGCTGGCCAAAGCTTTAGCCAATTGCCTCAAAATAGTTTTAAACTCTATTATAGTTCCCACCCAATTCGCTTTTGTCCCGAATAGGTTAATATTGGACAACATGACCATCGGCTTCGAAAGCATTGACACCCTTAATAGCAAAAAAAAAGGGGGAAAAGAAGGTTTTATTGCTATGAAGATCGACATGAGCAAGACTTACGACAGGGTTGAGTGGTTGTACATTGACAAAGTGATGGAGCAAATGGGTTTTAGCGAAAAGTGGAGAAGTAGCATATCTAACTTCTTGGCCTCGGTCCAATTCTCGGTGTTGGTGAATGGAATTCCTAGTGAAAGCTTCAAGCCAAAAAGAGGAATTAGGCAGGGAGATCCGCTCCCCCTTACATTTTCTTGA

Protein sequence

MSYKLAKALANCLKIVLNSIIVPTQFAFVPNRLILDNMTIGFESIDTLNSKKKGGKEGFIAMKIDMSKTYDRVEWLYIDKVMEQMGFSEKWRSSISNFLASVQFSVLVNGIPSESFKPKRGIRQGDPLPLTFS

Homology

[Close]

BLAST of Lag0016048 vs. NCBI nr
Match: XP_022157437.1 (uncharacterized protein LOC111024135 [Momordica charantia])

HSP 1 Score: 143.3 bits (360), Expect = 1.5e-30
Identity = 70/124 (56.45%), Postives = 92/124 (74.19%), Query Frame = 0


Query: 5   LAKALANCLKIVLNSIIVPTQFAFVPNRLILDNMTIGFESIDTLNSKKKGGKEGFIAMKI 64
           +AKALAN LK V++S+I PTQ AFVP R I DN  IGFE I+ + +K+  GK G +AMK+
Sbjct: 337 IAKALANRLKSVMDSVISPTQSAFVPGRSITDNAIIGFECINAIKNKRY-GKLGEVAMKL 396

Query: 65  DMSKTYDRVEWLYIDKVMEQMGFSEKWRSSISNFLASVQFSVLVNGIPSESFKPKRGIRQ 124
           DMSK YDRVEW Y+  +M +MGF+ +W   I N + SV+F+VL+NG+P + F P RG+RQ
Sbjct: 397 DMSKAYDRVEWQYLRGIMVKMGFANRWVDLIMNCVESVRFAVLINGVPGDEFTPNRGLRQ 456

Query: 125 GDPL 129
           GDPL
Sbjct: 457 GDPL 459

[Close]

BLAST of Lag0016048 vs. NCBI nr
Match: XP_042954551.1 (uncharacterized protein LOC122290956 [Carya illinoinensis])

HSP 1 Score: 142.5 bits (358), Expect = 2.6e-30
Identity = 65/124 (52.42%), Postives = 90/124 (72.58%), Query Frame = 0


Query: 5   LAKALANCLKIVLNSIIVPTQFAFVPNRLILDNMTIGFESIDTLNSKKKGGKEGFIAMKI 64
           +AK LAN LK +L  II  TQ AFVP R+I DN  + +E + ++ +++K G+E ++A+K+
Sbjct: 591 IAKVLANRLKTILPKIISDTQSAFVPGRMISDNTIVAYEILHSMQNRRKNGREAYMALKL 650

Query: 65  DMSKTYDRVEWLYIDKVMEQMGFSEKWRSSISNFLASVQFSVLVNGIPSESFKPKRGIRQ 124
           DMSK YDR+EW +I KV++QMGF  +W   I   + +V +S+LVNGIP  SFKP RGIRQ
Sbjct: 651 DMSKAYDRLEWPFIHKVLQQMGFVREWTDLIMQCIGTVSYSILVNGIPQPSFKPSRGIRQ 710

Query: 125 GDPL 129
           GDPL
Sbjct: 711 GDPL 714

[Close]

BLAST of Lag0016048 vs. NCBI nr
Match: XP_018853114.2 (uncharacterized protein LOC109015083 [Juglans regia])

HSP 1 Score: 140.6 bits (353), Expect = 9.9e-30
Identity = 66/124 (53.23%), Postives = 92/124 (74.19%), Query Frame = 0


Query: 5   LAKALANCLKIVLNSIIVPTQFAFVPNRLILDNMTIGFESIDTLNSKKKGGKEGFIAMKI 64
           ++K +AN LK++L  II PTQ AF+P RLI DN+ + FE++ T+N++ + GK G +A+K+
Sbjct: 483 ISKTMANRLKLILPEIISPTQSAFIPGRLISDNVIVAFEALHTMNNRLQ-GKIGHMALKL 542

Query: 65  DMSKTYDRVEWLYIDKVMEQMGFSEKWRSSISNFLASVQFSVLVNGIPSESFKPKRGIRQ 124
           DMSK YDR+EW +I  V+ +MGF EKW   I N + +V +S+LVNG P +SF P RGIRQ
Sbjct: 543 DMSKAYDRIEWPFIQAVLSKMGFPEKWIKMIMNCVETVSYSLLVNGSPQQSFSPSRGIRQ 602

Query: 125 GDPL 129
           GDPL
Sbjct: 603 GDPL 605

[Close]

BLAST of Lag0016048 vs. NCBI nr
Match: KAF5458478.1 (hypothetical protein F2P56_022503 [Juglans regia])

HSP 1 Score: 140.6 bits (353), Expect = 9.9e-30
Identity = 66/124 (53.23%), Postives = 92/124 (74.19%), Query Frame = 0


Query: 5   LAKALANCLKIVLNSIIVPTQFAFVPNRLILDNMTIGFESIDTLNSKKKGGKEGFIAMKI 64
           ++K +AN LK++L  II PTQ AF+P RLI DN+ + FE++ T+N++ + GK G +A+K+
Sbjct: 484 ISKTMANRLKLILPEIISPTQSAFIPGRLISDNVIVAFEALHTMNNRLQ-GKIGHMALKL 543

Query: 65  DMSKTYDRVEWLYIDKVMEQMGFSEKWRSSISNFLASVQFSVLVNGIPSESFKPKRGIRQ 124
           DMSK YDR+EW +I  V+ +MGF EKW   I N + +V +S+LVNG P +SF P RGIRQ
Sbjct: 544 DMSKAYDRIEWPFIQAVLSKMGFPEKWIKMIMNCVETVSYSLLVNGSPQQSFSPSRGIRQ 603

Query: 125 GDPL 129
           GDPL
Sbjct: 604 GDPL 606

[Close]

BLAST of Lag0016048 vs. NCBI nr
Match: XP_042974779.1 (uncharacterized protein LOC122306417 [Carya illinoinensis])

HSP 1 Score: 139.8 bits (351), Expect = 1.7e-29
Identity = 63/124 (50.81%), Postives = 89/124 (71.77%), Query Frame = 0


Query: 5   LAKALANCLKIVLNSIIVPTQFAFVPNRLILDNMTIGFESIDTLNSKKKGGKEGFIAMKI 64
           +AK LAN LK +L  +I  TQ AFVP R+I DN  + +E + ++ +++K G+E ++A+K+
Sbjct: 494 IAKVLANRLKTILPKLISDTQSAFVPGRMISDNTIVAYELLHSMQNRRKNGREAYMALKL 553

Query: 65  DMSKTYDRVEWLYIDKVMEQMGFSEKWRSSISNFLASVQFSVLVNGIPSESFKPKRGIRQ 124
           DMSK YDR+EW +I +V++QMGF  +W   I     +V +S+LVNGIP  SFKP RGIRQ
Sbjct: 554 DMSKAYDRLEWSFIHQVLQQMGFDREWTDLIMQCTGTVTYSILVNGIPQPSFKPSRGIRQ 613

Query: 125 GDPL 129
           GDPL
Sbjct: 614 GDPL 617

[Close]

BLAST of Lag0016048 vs. ExPASy Swiss-Prot
Match: P16423 (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 9.7e-04
Identity = 42/133 (31.58%), Postives = 61/133 (45.86%), Query Frame = 0


Query: 5   LAKALANCLKIVLNSII--VPTQFAFVPNRLILDNMTIGFESID-TLNSKKKGGKEGFIA 64
           L + L   L   LNS I   P Q  F+P     DN TI    +D  L    K  +  +IA
Sbjct: 424 LVRQLNAILATRLNSSINWDPRQRGFLPTDGCADNATI----VDLVLRHSHKHFRSCYIA 483

Query: 65  MKIDMSKTYDRVEWLYIDKVMEQMGFSEKWRSSISNFLASVQFSVLVNGIPSESFKPKRG 124
             +D+SK +D +    I   +   G  + +   + N       S+  +G  SE F P RG
Sbjct: 484 -NLDVSKAFDSLSHASIYDTLRAYGAPKGFVDYVQNTYEGGGTSLNGDGWSSEEFVPARG 543

Query: 125 IRQGDPL-PLTFS 134
           ++QGDPL P+ F+
Sbjct: 544 VKQGDPLSPILFN 551

The following BLAST results are available for this feature:

BLAST of Lag0016048 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157437.1	1.5e-30	56.45	uncharacterized protein LOC111024135 [Momordica charantia]	[more]
XP_042954551.1	2.6e-30	52.42	uncharacterized protein LOC122290956 [Carya illinoinensis]	[more]
XP_018853114.2	9.9e-30	53.23	uncharacterized protein LOC109015083 [Juglans regia]	[more]
KAF5458478.1	9.9e-30	53.23	hypothetical protein F2P56_022503 [Juglans regia]	[more]
XP_042974779.1	1.7e-29	50.81	uncharacterized protein LOC122306417 [Carya illinoinensis]	[more]

BLAST of Lag0016048 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16423	9.7e-04	31.58	Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 5..128 e-value: 9.4E-17 score: 61.3
None	No IPR available	PANTHER	PTHR19446:SF440	SUBFAMILY NOT NAMED	coord: 4..109
None	No IPR available	PANTHER	PTHR19446	REVERSE TRANSCRIPTASES	coord: 4..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0016048.1	Lag0016048.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0140640	catalytic activity, acting on a nucleic acid