Lag0016013 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016013
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionUbiquitin thioesterase OTU1
Locationchr12: 31489396 .. 31490329 (-)
RNA-Seq ExpressionLag0016013
SyntenyLag0016013
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCATGATAAACACAAGCCTACTGAGCTAAGACAGGTTCTGATTCATAATATTTCATTGTTATTGGAACTATACTTCACTGCTTTTTATTATGCATCACTATTTAGGAGTTATTTTCAAATTCTAAATTCCTTGAAGGAAGATATACATCCAGGTTAGAAGACTTGCATTTTTTCCCTTATAGTTAGACGTACTGAAAAAGAAGTTGCAGAAATTAAAAATGAGAATGTCAAAAGAACAAGGAAAAGGAAAAGGCGTGCTTTTTTTTTTTGAACAAAAAGGAAAAGGCATGCTTAATCAATAGAATCATAGAGCTGAAAGAAATCTTCTCATTCCATAGGATAGCTACTTTTTGTATAGAATCACATTTGTATTCTTTACTCTGAATATCAAACACAATTGATTCTCTTGAAGGTCATAGCTGCAACAATAGCTAGTGATCCAACAAAGTATTCTGAAGCATATCTTGGAAAGCCAAATGAGGAGTGTTGTGCTTGGATTCTTGACTCGGAGAAGTGGGGAGGTTTGTATCAATGTTATCATTGCTTAATGGAAAGGGTTTTATGAGATTTTCTTGTTGTAAATCTGTAGAACTTCCAAAATATATAGTTACCCTGTTGTTTCTTATTTTCTAGCATTAGATGATGCGATAGGTGTTTTGTTGAACCTCCTCATGGATTTCTTGAATATGTTGCGTTGCACTCTTGTCCACTCACATTCTATAGTCTTCTATGAAACATTGGGAGTGTATGTATTGACCTACTGTTATTGCAGGCTACGTGGATGGCTCTTGATCACACACTTCAACAACCCACTGACTGCAACTTCTCAGCTCACATGCAAAAGCCCCAAAATCAACTTTACACTTCAATCCAAGAATACCAAAACAGAGAACTTCTCTGTACTTGCGAAGAAGAAGAAAAACACGTAG

mRNA sequence

ATGGACCATGATAAACACAAGCCTACTGAGCTAAGACAGGTCATAGCTGCAACAATAGCTAGTGATCCAACAAAGTATTCTGAAGCATATCTTGGAAAGCCAAATGAGGAGTGTTGTGCTTGGATTCTTGACTCGGAGAAGTGGGGAGCATTAGATGATGCGATAGGTGTTTTGTTGAACCTCCTCATGGATTTCTTGAATATGTTGCGTTGCACTCTTGTCCACTCACATTCTATAGTCTTCTATGAAACATTGGGAGTGTATGTATTGACCTACTGTTATTGCAGGCTACGTGGATGGCTCTTGATCACACACTTCAACAACCCACTGACTGCAACTTCTCAGCTCACATGCAAAAGCCCCAAAATCAACTTTACACTTCAATCCAAGAATACCAAAACAGAGAACTTCTCTGTACTTGCGAAGAAGAAGAAAAACACGTAG

Coding sequence (CDS)

ATGGACCATGATAAACACAAGCCTACTGAGCTAAGACAGGTCATAGCTGCAACAATAGCTAGTGATCCAACAAAGTATTCTGAAGCATATCTTGGAAAGCCAAATGAGGAGTGTTGTGCTTGGATTCTTGACTCGGAGAAGTGGGGAGCATTAGATGATGCGATAGGTGTTTTGTTGAACCTCCTCATGGATTTCTTGAATATGTTGCGTTGCACTCTTGTCCACTCACATTCTATAGTCTTCTATGAAACATTGGGAGTGTATGTATTGACCTACTGTTATTGCAGGCTACGTGGATGGCTCTTGATCACACACTTCAACAACCCACTGACTGCAACTTCTCAGCTCACATGCAAAAGCCCCAAAATCAACTTTACACTTCAATCCAAGAATACCAAAACAGAGAACTTCTCTGTACTTGCGAAGAAGAAGAAAAACACGTAG

Protein sequence

MDHDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWGALDDAIGVLLNLLMDFLNMLRCTLVHSHSIVFYETLGVYVLTYCYCRLRGWLLITHFNNPLTATSQLTCKSPKINFTLQSKNTKTENFSVLAKKKKNT
Homology
[Close]
BLAST of Lag0016013 vs. NCBI nr
Match: TYK23120.1 (ubiquitin thioesterase OTU1 [Cucumis melo var. makuwa])

HSP 1 Score: 99.0 bits (245), Expect = 3.7e-17
Identity = 43/49 (87.76%), Postives = 47/49 (95.92%), Query Frame = 0 

Query: 1   MDHDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWG 50
           MDHDKHK TELRQVIAAT+ASDPT+YSEA+LGKPNEE C+WILDSEKWG
Sbjct: 191 MDHDKHKATELRQVIAATVASDPTRYSEAFLGKPNEEYCSWILDSEKWG 239

[Close]
BLAST of Lag0016013 vs. NCBI nr
Match: XP_008447742.1 (PREDICTED: ubiquitin thioesterase OTU1 [Cucumis melo])

HSP 1 Score: 99.0 bits (245), Expect = 3.7e-17
Identity = 43/49 (87.76%), Postives = 47/49 (95.92%), Query Frame = 0 

Query: 1  MDHDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWG 50
          MDHDKHK TELRQVIAAT+ASDPT+YSEA+LGKPNEE C+WILDSEKWG
Sbjct: 25 MDHDKHKATELRQVIAATVASDPTRYSEAFLGKPNEEYCSWILDSEKWG 73

[Close]
BLAST of Lag0016013 vs. NCBI nr
Match: KAG2682259.1 (hypothetical protein I3760_11G183500 [Carya illinoinensis] >KAG6689659.1 hypothetical protein I3842_11G186300 [Carya illinoinensis] >KAG6689660.1 hypothetical protein I3842_11G186300 [Carya illinoinensis] >KAG6689661.1 hypothetical protein I3842_11G186300 [Carya illinoinensis] >KAG7957599.1 hypothetical protein I3843_11G184200 [Carya illinoinensis])

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-16
Identity = 46/65 (70.77%), Postives = 52/65 (80.00%), Query Frame = 0 

Query: 1  MDHDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWGALDDAIGVLLN 60
          MDHDKHK  ELRQVIAAT+ASDP KYSEA+LGKPNEE CAWILDSEKWG       + L+
Sbjct: 25 MDHDKHKAPELRQVIAATVASDPAKYSEAFLGKPNEEYCAWILDSEKWGG-----AIELS 84

Query: 61 LLMDF 66
          +L D+
Sbjct: 85 ILADY 84

[Close]
BLAST of Lag0016013 vs. NCBI nr
Match: XP_042948137.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2-like [Carya illinoinensis] >XP_042948138.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2-like [Carya illinoinensis] >KAG6637596.1 hypothetical protein CIPAW_11G189300 [Carya illinoinensis] >KAG6637597.1 hypothetical protein CIPAW_11G189300 [Carya illinoinensis])

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-16
Identity = 46/65 (70.77%), Postives = 52/65 (80.00%), Query Frame = 0 

Query: 1  MDHDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWGALDDAIGVLLN 60
          MDHDKHK  ELRQVIAAT+ASDP KYSEA+LGKPNEE CAWILDSEKWG       + L+
Sbjct: 25 MDHDKHKAPELRQVIAATVASDPAKYSEAFLGKPNEEYCAWILDSEKWGG-----AIELS 84

Query: 61 LLMDF 66
          +L D+
Sbjct: 85 ILADY 84

[Close]
BLAST of Lag0016013 vs. NCBI nr
Match: XP_038897758.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 [Benincasa hispida] >XP_038897759.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 [Benincasa hispida] >XP_038897760.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 [Benincasa hispida])

HSP 1 Score: 97.1 bits (240), Expect = 1.4e-16
Identity = 42/49 (85.71%), Postives = 46/49 (93.88%), Query Frame = 0 

Query: 1  MDHDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWG 50
          MDHDKHK TELRQVIAAT+ASDPT+YSEA+LGKPNEE C+WILD EKWG
Sbjct: 25 MDHDKHKATELRQVIAATVASDPTRYSEAFLGKPNEEYCSWILDPEKWG 73

[Close]
BLAST of Lag0016013 vs. ExPASy Swiss-Prot
Match: Q9LPT6 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=OTU2 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.6e-12
Identity = 36/65 (55.38%), Postives = 47/65 (72.31%), Query Frame = 0 

Query: 1  MDHDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWGALDDAIGVLLN 60
          MD DK+K  ELRQVIAA +AS+  KY+EA+LGK NEE CAWIL+ +KWG       + L+
Sbjct: 25 MDKDKNKAPELRQVIAAAVASNKEKYNEAFLGKLNEEYCAWILNPDKWGG-----AIELS 84

Query: 61 LLMDF 66
          +L D+
Sbjct: 85 ILADY 84

[Close]
BLAST of Lag0016013 vs. ExPASy Swiss-Prot
Match: Q567B1 (Ubiquitin thioesterase OTU1 OS=Danio rerio OX=7955 GN=yod1 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.6e-07
Identity = 25/47 (53.19%), Postives = 31/47 (65.96%), Query Frame = 0 

Query: 3   HDKHKPTELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWG 50
           +D    +E+R +IA  +ASDPT YSEA LGK NEE C WI   + WG
Sbjct: 127 YDPACASEMRGLIAQIVASDPTAYSEAVLGKTNEEYCTWIRRDDTWG 173

[Close]
BLAST of Lag0016013 vs. ExPASy Swiss-Prot
Match: Q32Q05 (Ubiquitin thioesterase OTU1 OS=Rattus norvegicus OX=10116 GN=Yod1 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.8e-06
Identity = 23/40 (57.50%), Postives = 28/40 (70.00%), Query Frame = 0 

Query: 10  ELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWG 50
           E+R++IA  +ASDP  YSEA LGK NEE C WI   + WG
Sbjct: 176 EMRRLIAQIVASDPDLYSEAILGKTNEEYCDWIRRDDTWG 215

[Close]
BLAST of Lag0016013 vs. ExPASy Swiss-Prot
Match: Q8CB27 (Ubiquitin thioesterase OTU1 OS=Mus musculus OX=10090 GN=Yod1 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 3.0e-06
Identity = 22/40 (55.00%), Postives = 28/40 (70.00%), Query Frame = 0 

Query: 10  ELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWG 50
           E+R++IA  +ASDP  YSEA LGK NE+ C WI   + WG
Sbjct: 176 EMRRLIAQIVASDPVLYSEAILGKTNEDYCDWIRRDDTWG 215

[Close]
BLAST of Lag0016013 vs. ExPASy Swiss-Prot
Match: Q05B57 (Ubiquitin thioesterase OTU1 OS=Bos taurus OX=9913 GN=YOD1 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 3.9e-06
Identity = 23/40 (57.50%), Postives = 27/40 (67.50%), Query Frame = 0 

Query: 10  ELRQVIAATIASDPTKYSEAYLGKPNEECCAWILDSEKWG 50
           E+R+ IA  +ASDP  YSEA LGK NEE C WI   + WG
Sbjct: 181 EMRRFIAQIVASDPDFYSEAILGKTNEEYCDWIKRDDTWG 220

The following BLAST results are available for this feature:
BLAST of Lag0016013 vs. NCBI nr
Match NameE-valueIdentityDescription
TYK23120.13.7e-1787.76ubiquitin thioesterase OTU1 [Cucumis melo var. makuwa][more]
XP_008447742.13.7e-1787.76PREDICTED: ubiquitin thioesterase OTU1 [Cucumis melo][more]
KAG2682259.11.1e-1670.77hypothetical protein I3760_11G183500 [Carya illinoinensis] >KAG6689659.1 hypothe... [more]
XP_042948137.11.1e-1670.77OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2-like [Carya illinoinen... [more]
XP_038897758.11.4e-1685.71OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 [Benincasa hispida] >X... [more]
BLAST of Lag0016013 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LPT61.6e-1255.38OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 2 OS=Arabidopsis thalian... [more]
Q567B13.6e-0753.19Ubiquitin thioesterase OTU1 OS=Danio rerio OX=7955 GN=yod1 PE=2 SV=1[more]
Q32Q051.8e-0657.50Ubiquitin thioesterase OTU1 OS=Rattus norvegicus OX=10116 GN=Yod1 PE=2 SV=1[more]
Q8CB273.0e-0655.00Ubiquitin thioesterase OTU1 OS=Mus musculus OX=10090 GN=Yod1 PE=1 SV=1[more]
Q05B573.9e-0657.50Ubiquitin thioesterase OTU1 OS=Bos taurus OX=9913 GN=YOD1 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.70.80coord: 1..54
e-value: 2.1E-13
score: 52.5
NoneNo IPR availablePANTHERPTHR13312:SF0UBIQUITIN THIOESTERASE OTU1coord: 1..50
IPR039138Ubiquitin thioesterase OTU1/2/3PANTHERPTHR13312HIV-INDUCED PROTEIN-7-LIKE PROTEASEcoord: 1..50

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016013.1Lag0016013.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
biological_process GO:0016579 protein deubiquitination
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004843 thiol-dependent deubiquitinase
molecular_function GO:0101005 deubiquitinase activity