Lag0015990 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0015990
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr12: 30900054 .. 30900608 (-)
RNA-Seq ExpressionLag0015990
SyntenyLag0015990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAGATAGGTTTTAGCTCAGCTTGGATAAAGAAGATTGGCATGTGTGTGGAATCTGTATCCTTTTCGATCCTTATTAATGGTAGTTGTTATGAATCTTTTAAGCCCTCACGAGGGCTTAGACAGGGCGATCCGTTATCTCCTTATTTATTTCTCATGTGTGATGAGGGACTATCTAGCCTCCTAAAAAAGGCCGATATATCTCAAAATATTTCAGGTCTTAGAATTGCAAAAACGGCACCACCTATCTCGCACCTCTTTTTTGCAGATGATAGTTTACTTTTTCTCAAAGCGCGAAAGGAGGAATTCGATGAGATGCAACGAATCGTGAAAGTATATGAAGCAGCCACAGGACAGATCGTTAATTTTGATAAATCGGAGATAGCTTTTGGGGCCGGAGTGGCAAATGAAGCAAAAGGCTTTTTAGCCAACATTTTAAAAGTCAAGATAGTCCAAGACCATGGAAAGTATCTTGGATGTCCGTCTTCCCTATCCAGAAATAAGAAAGCTTCTCTGAACTACATAACATCTAAAGTTCAGAATGTGTTGTAG

mRNA sequence

ATGGCTAAGATAGGTTTTAGCTCAGCTTGGATAAAGAAGATTGGCATGTGTGTGGAATCTGTATCCTTTTCGATCCTTATTAATGGTAGTTGTTATGAATCTTTTAAGCCCTCACGAGGGCTTAGACAGGGCGATCCGTTATCTCCTTATTTATTTCTCATGTGTGATGAGGGACTATCTAGCCTCCTAAAAAAGGCCGATATATCTCAAAATATTTCAGGTCTTAGAATTGCAAAAACGGCACCACCTATCTCGCACCTCTTTTTTGCAGATGATAGTTTACTTTTTCTCAAAGCGCGAAAGGAGGAATTCGATGAGATGCAACGAATCGTGAAAGTATATGAAGCAGCCACAGGACAGATCGTTAATTTTGATAAATCGGAGATAGCTTTTGGGGCCGGAGTGGCAAATGAAGCAAAAGGCTTTTTAGCCAACATTTTAAAAGTCAAGATAGTCCAAGACCATGGAAAGTATCTTGGATGTCCGTCTTCCCTATCCAGAAATAAGAAAGCTTCTCTGAACTACATAACATCTAAAGTTCAGAATGTGTTGTAG

Coding sequence (CDS)

ATGGCTAAGATAGGTTTTAGCTCAGCTTGGATAAAGAAGATTGGCATGTGTGTGGAATCTGTATCCTTTTCGATCCTTATTAATGGTAGTTGTTATGAATCTTTTAAGCCCTCACGAGGGCTTAGACAGGGCGATCCGTTATCTCCTTATTTATTTCTCATGTGTGATGAGGGACTATCTAGCCTCCTAAAAAAGGCCGATATATCTCAAAATATTTCAGGTCTTAGAATTGCAAAAACGGCACCACCTATCTCGCACCTCTTTTTTGCAGATGATAGTTTACTTTTTCTCAAAGCGCGAAAGGAGGAATTCGATGAGATGCAACGAATCGTGAAAGTATATGAAGCAGCCACAGGACAGATCGTTAATTTTGATAAATCGGAGATAGCTTTTGGGGCCGGAGTGGCAAATGAAGCAAAAGGCTTTTTAGCCAACATTTTAAAAGTCAAGATAGTCCAAGACCATGGAAAGTATCTTGGATGTCCGTCTTCCCTATCCAGAAATAAGAAAGCTTCTCTGAACTACATAACATCTAAAGTTCAGAATGTGTTGTAG

Protein sequence

MAKIGFSSAWIKKIGMCVESVSFSILINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLSSLLKKADISQNISGLRIAKTAPPISHLFFADDSLLFLKARKEEFDEMQRIVKVYEAATGQIVNFDKSEIAFGAGVANEAKGFLANILKVKIVQDHGKYLGCPSSLSRNKKASLNYITSKVQNVL
Homology
BLAST of Lag0015990 vs. NCBI nr
Match: XP_019170410.1 (PREDICTED: uncharacterized protein LOC109165884 [Ipomoea nil])

HSP 1 Score: 188.3 bits (477), Expect = 5.7e-44
Identity = 89/178 (50.00%), Postives = 123/178 (69.10%), Query Frame = 0

Query: 4   IGFSSAWIKKIGMCVESVSFSILINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLSSLL 63
           +GF   W+K + MCV +VS+S+LINGS  E   P+RGLRQGDPLSPYLF++C EGLS LL
Sbjct: 358 LGFDDRWVKFLMMCVSTVSYSVLINGSKSEQITPTRGLRQGDPLSPYLFIICAEGLSILL 417

Query: 64  KKADISQNISGLRIAKTAPPISHLFFADDSLLFLKARKEEFDEMQRIVKVYEAATGQIVN 123
           K+A++  +I G R+A+ AP I+HLFFADDSLLF KA  +E   ++R +  YE  +GQ VN
Sbjct: 418 KQAEVKGDIHGCRVARGAPSITHLFFADDSLLFFKANNQEAGAIKRCLTTYETMSGQAVN 477

Query: 124 FDKSEIAFGAGVANEAKGFLANILKVKIVQDHGKYLGCPSSLSRNKKASLNYITSKVQ 182
           + KS I F    + E +  + +IL V   Q+ GKYLG PS + RN++ + +YI  K++
Sbjct: 478 YHKSNICFSKNTSEEVREEIVDILGVVRAQNFGKYLGLPSFVGRNRRQAFSYIEDKIK 535

BLAST of Lag0015990 vs. NCBI nr
Match: XP_019198168.1 (PREDICTED: uncharacterized protein LOC109191994 [Ipomoea nil])

HSP 1 Score: 187.6 bits (475), Expect = 9.8e-44
Identity = 92/181 (50.83%), Postives = 124/181 (68.51%), Query Frame = 0

Query: 1   MAKIGFSSAWIKKIGMCVESVSFSILINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLS 60
           M  +GF   W++ I  CV +VS+++L+NGS  E   P+RGLRQGDPLSPYLF++C EGLS
Sbjct: 526 MLALGFDVGWVQLIMNCVSTVSYNLLVNGSRSEPILPTRGLRQGDPLSPYLFIICAEGLS 585

Query: 61  SLLKKADISQNISGLRIAKTAPPISHLFFADDSLLFLKARKEEFDEMQRIVKVYEAATGQ 120
            LL++A     I G R+A+ APPISHLFFADDSLLF KA  +E  E++R + VYE  +GQ
Sbjct: 586 LLLQQAHNRGLIHGCRVARGAPPISHLFFADDSLLFFKANVQEASEIKRCLSVYEDLSGQ 645

Query: 121 IVNFDKSEIAFGAGVANEAKGFLANILKVKIVQDHGKYLGCPSSLSRNKKASLNYITSKV 180
           +VN+ KS I +    +N +K  +A +L V    + GKYLG PS + RNKKA+  YI  K+
Sbjct: 646 VVNYHKSSICYSRNTSNASKEVVAQVLGVGQAPNFGKYLGLPSFVGRNKKAAFAYIEDKI 705

Query: 181 Q 182
           +
Sbjct: 706 R 706

BLAST of Lag0015990 vs. NCBI nr
Match: XP_019169907.1 (PREDICTED: uncharacterized protein LOC109165547 [Ipomoea nil])

HSP 1 Score: 186.0 bits (471), Expect = 2.8e-43
Identity = 91/181 (50.28%), Postives = 124/181 (68.51%), Query Frame = 0

Query: 1   MAKIGFSSAWIKKIGMCVESVSFSILINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLS 60
           M  +GF   W++ I  CV +VS+++L+NGS  E   P+RGLRQGDPLSPYLF++C EGLS
Sbjct: 301 MLALGFDVGWVQLIMNCVSTVSYNLLVNGSRSEPILPTRGLRQGDPLSPYLFIICAEGLS 360

Query: 61  SLLKKADISQNISGLRIAKTAPPISHLFFADDSLLFLKARKEEFDEMQRIVKVYEAATGQ 120
            LL++A     I G R+A+ APPISHLFFADDSLLF KA  +E  E++R + VYE  +G+
Sbjct: 361 LLLQQAHNRGLIHGCRVARGAPPISHLFFADDSLLFFKANVQEASEIKRCLSVYEDLSGK 420

Query: 121 IVNFDKSEIAFGAGVANEAKGFLANILKVKIVQDHGKYLGCPSSLSRNKKASLNYITSKV 180
           +VN+ KS I +    +N +K  +A +L V    + GKYLG PS + RNKKA+  YI  K+
Sbjct: 421 VVNYHKSSICYSRNTSNASKEVVAQVLGVGQAPNFGKYLGLPSFVGRNKKAAFAYIEDKI 480

Query: 181 Q 182
           +
Sbjct: 481 R 481

BLAST of Lag0015990 vs. NCBI nr
Match: XP_019184158.1 (PREDICTED: uncharacterized protein LOC109179042 [Ipomoea nil])

HSP 1 Score: 186.0 bits (471), Expect = 2.8e-43
Identity = 93/181 (51.38%), Postives = 119/181 (65.75%), Query Frame = 0

Query: 1   MAKIGFSSAWIKKIGMCVESVSFSILINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLS 60
           + ++GF   W++ + +CV SV + +LING   E   P+RGLRQGDPLSPYLF++C EGLS
Sbjct: 77  LRRLGFDDRWVQLVMLCVRSVRYKVLINGRPSEEIVPTRGLRQGDPLSPYLFIICAEGLS 136

Query: 61  SLLKKADISQNISGLRIAKTAPPISHLFFADDSLLFLKARKEEFDEMQRIVKVYEAATGQ 120
            LL+ +     I G R+A+ AP ISHLFFADDSLLF KA  +E  E++R + VYEA +GQ
Sbjct: 137 LLLQDSQTRGLIHGCRVARGAPSISHLFFADDSLLFFKANLQETMEVKRCLGVYEAYSGQ 196

Query: 121 IVNFDKSEIAFGAGVANEAKGFLANILKVKIVQDHGKYLGCPSSLSRNKKASLNYITSKV 180
            VNF+KS I+F     NE K  LA  L V    D GKYLG PS + RNKK    YI  K+
Sbjct: 197 AVNFNKSSISFSRNTRNEVKDLLAGALGVAQADDFGKYLGLPSVIGRNKKLVFAYIEQKM 256

Query: 181 Q 182
           +
Sbjct: 257 K 257

BLAST of Lag0015990 vs. NCBI nr
Match: XP_019175839.1 (PREDICTED: uncharacterized protein LOC109171159 [Ipomoea nil])

HSP 1 Score: 186.0 bits (471), Expect = 2.8e-43
Identity = 88/178 (49.44%), Postives = 122/178 (68.54%), Query Frame = 0

Query: 4   IGFSSAWIKKIGMCVESVSFSILINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLSSLL 63
           +GF   W+K + MCV +VS+S+LINGS  E   P+RGLRQGDPLSPYLF++C EGLS LL
Sbjct: 509 LGFDGRWVKLLMMCVSTVSYSVLINGSKTEQITPTRGLRQGDPLSPYLFIICAEGLSILL 568

Query: 64  KKADISQNISGLRIAKTAPPISHLFFADDSLLFLKARKEEFDEMQRIVKVYEAATGQIVN 123
           K+A++  +I G R+A+ AP I+HLFFA DSLLF KA  +E   ++R +  YE  +GQ VN
Sbjct: 569 KQAEVKGDIHGCRVARAAPSITHLFFAGDSLLFFKANNQEAGAIKRCLTTYETMSGQAVN 628

Query: 124 FDKSEIAFGAGVANEAKGFLANILKVKIVQDHGKYLGCPSSLSRNKKASLNYITSKVQ 182
           + KS I F    + E +  +A+IL V   Q+ GKYLG P  + RN++ + +YI  K++
Sbjct: 629 YHKSNICFSKNTSEEVREEIADILGVVQAQNFGKYLGLPFFVGRNRRQAFSYIEDKIK 686

BLAST of Lag0015990 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.9e-14
Identity = 35/68 (51.47%), Postives = 48/68 (70.59%), Query Frame = 0

Query: 26 LINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLSSLLKKADISQNISGLRIAKTAPPIS 85
          +ING+      PSRGLRQGDPLSPYLF++C E LS L ++A     + G+R++  +P I+
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 86 HLFFADDS 94
          HL FADD+
Sbjct: 73 HLLFADDT 80

BLAST of Lag0015990 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 5.3e-08
Identity = 44/160 (27.50%), Postives = 75/160 (46.88%), Query Frame = 0

Query: 1   MAKIGFSSAWIKKIGMCVESVSFSILINGSCYESFKPSRGLRQGDPLSPYLFLMCDEGLS 60
           + + G    ++  I         +I +NG   E+     G RQG PLSPYLF +  E L+
Sbjct: 624 LERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLA 683

Query: 61  SLLKKADISQNISGLRIAKTAPPISHLFFADDSLLFLKARKEEFDEMQRIVKVYEAATGQ 120
             +++    + I G++I K    IS L  ADD ++++   K    E+  ++  +    G 
Sbjct: 684 RAIRQ---QKEIKGIQIGKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVGY 743

Query: 121 IVNFDKSEIAFGAGVANEAKGFLANILKVKIVQDHGKYLG 161
            +N +KS +AF      +A+  +       IV ++ KYLG
Sbjct: 744 KINSNKS-MAFLYTKNKQAEKEIRETTPFSIVTNNIKYLG 777

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_019170410.15.7e-4450.00PREDICTED: uncharacterized protein LOC109165884 [Ipomoea nil][more]
XP_019198168.19.8e-4450.83PREDICTED: uncharacterized protein LOC109191994 [Ipomoea nil][more]
XP_019169907.12.8e-4350.28PREDICTED: uncharacterized protein LOC109165547 [Ipomoea nil][more]
XP_019184158.12.8e-4351.38PREDICTED: uncharacterized protein LOC109179042 [Ipomoea nil][more]
XP_019175839.12.8e-4349.44PREDICTED: uncharacterized protein LOC109171159 [Ipomoea nil][more]
Match NameE-valueIdentityDescription
P925552.9e-1451.47Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
P113695.3e-0827.50LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 4..161
e-value: 6.6E-15
score: 55.2
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 1..147
score: 8.629629
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 1..182
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 1..182
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 5..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0015990.1Lag0015990.1mRNA