Lag0015918 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0015918
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase
Locationchr12: 29155076 .. 29158462 (-)
RNA-Seq ExpressionLag0015918
SyntenyLag0015918
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGATCCGCCTGGGGTACGGTTTGAGCTTGATCCAGAAATTGAAAGGACATTTAGGAACAGAAGGAGGGAGCAGCGTAGAAACCAGATGGAGAACGCCCCGCAACTTCCGCAGGTTCCTGAAGGTCCAGCAGCAGCAAACCCCAGCAGAATCCGTTGCTGCAGCAAAACCCACTGTTTGAGCAAAATGAGCAGCGAAATAATCAGGCTGAGAATCCTATCTTGATAGCGAACGATAGGACCAGAGCATTCGAGCATATGCTGTCCCGATGTTTAATGAGTTGAATCCAGGGATTGCACGTCCCCATATCCAAGCGGCAAATTTTGAAATGAAACCGGTAATGTTTCAGATGTTGCAAACCGTGGGGCAATTCCATGGTTTGTCATCTGAAGACCCTCATTTACATCTTAAGTCTTTTCTAGGAGTTAGTGATTCTTTTGTAATTCAAAGAGTGCCTAGAGATGCTCTTAGATTAACTTTGTTCCGTATTCTCTTAGAGATGGAGCAAAGTCATGGTTAAACTCTTTTGCTCCAGGATCAATTAGGACGTGGGATGAGTTAGCTGAAAATTTTTTGAGTAAATATTTCCCACCTAATAGAAATGCAAATTAAGGAGTGAAATAGTATGGTTTAGGCCACTTGAGGATGAGACTTTTAGTGAGGCTTGGGAGAGGTTTAAGGAGCTTTTACGAAAGTGTCCCCACCATGGGTTACCTCATTGTATTCAAATGGAAACATTTTACAATGGTTTAAATGGAGTAACCCAAGGTATGGTCGATGCCTCGGCTGGAGGGGCCCTTTTGGCAAAAACTTTTGATGAAGCCTATGAAATTTTAGAAAGAATATCATTAATAGTTGTCAGTGGTCGGATGTTAGAGGCACAAATAAAAAGGTTAAGAGTGTATTAGAGGTTGATGGTGTGTCCACTATTAGGGCCGATCTTGCTATGATTGCTAATGCTCTTAAGAATGTGACAGTGATTAGTCATCAGCAGCCACCAGCTATGGAGCCTGCAGCAGTGGTGAACCAAGTCACGGACGAAGCATGTGTCTATTGCGGTGAAGACCACAACTACGAGTTTTGCCCCAGCAATCCAGCTTCTGTGTTTTTTGTAGGTAATCAGAGGAACAACCCTTATTCTAACTTTTATAATCCAGGTTGGCGCAACCACCCCAACTTCTCATGGGGAGTCAAGGAAATAACGTACAAGCGCAACAGAAGATGAACCAGCCGGGATTTGCTAAAGCGCAGGTAATGCCCCAGCAAAATAACCGGCTTTGCCCCAGCAAAATTCGGGAAATCCTCTCGAGGCAATGATGAAAGAATTTATGGCTCGCACAGATGCCGCAATTCAAAGTAATCAAGCTTCGATGAGGGCCCTGGAATTGCAAGTGGGTTAGCTAGCTAATGAGCTCAAGGCGAGGCCTCAAGGGAAACTTCCTTCGGATACTGAACACCCTCGAAGGGAAGGTAAGGAGCAGGTAAAGGCAGTAACTCTTAGGAGTGGTAAGCCACTAGAAGAGTCTAGAAAGACCCAGGATTTAAATAGTAATAGAAATAATAATGTTGTTATTGAGAAAGAGTTGGAGTCTGGACAGGGTGCTGGAGGCAGCAAAGAGAATGCTGGAGCATCTGGTTCTGTGCCAGATGTAGAAGCACCATATGTGCCGCCCCCACCTTATGTACCACCTCTACCTTTTCCACAAAGGCAAAAGCCTAAGAATAGGATGGTCAATTTAAAAAGTTTTTAGAGATTCTTAAGCAGTTGCATATAAATATCCCTTTAGTAGAAGCTATAGAGCAAATGCCTAATTATGCTAAATTTCTTAAGGATATTTTGACTAAAAAGAAAAGGTTAGGTGAGTTTGAAACTGTATCTCTTACTGAGGAGTGTAGTGTGATTATCAAGAATGGTCTACCCCCAAGGCTAAGGATCCAGGGTCATTTACCATACCTGTGTCTATAGGTGGAAAGGAATTAGGTAGAGCACTCTGTGATTTAGGTGCAAGCATTAACCTTATGCCTCTTTCGGTCTATCGGAAGTTAGGTATTGGTGAAGCTAGTCCTACCATAGTTACACTCCAACTAGCTGATAGGTCTATCACATATCCAGAGGGTAAAATTGAGGATGTCTTAGTAAAAGTGGATAAATTCATATTTCCGGTTGATTTTATTATTTTAGACTATGAGGCTGATAAAGATGTCCCAATTATTCTAGGTCGTCCATTTTTGGCTACTGGTAGGGCATTAATAGATGTTCAAAAAGGAGAATTAACAATGAGAGTCTGTAATGAGGAAGTGAAATTTAATGTGTTTAAAGCCATGAAATATCCAGACGAAATGGAGGATTGCTCCTTCATTAGGATTCTGGAGAGCACAGTTATTGAGACAGCAATACAGGATTCGGCTGATAAGCATTCAGAAAAGCATGGAGAGGTTAGTGTAGAAGATTTTGAATCTTGTTCTTTAGATAGAAGAAATGAAAAAGAATTGTTTAGGTGTGAGGATGTTTTTGAGTCTTTAGATTTAGATCAAAGAAAGGCTCCCCCAATTAAACCATCCCTGATGAGGCACCTACTTTAGATTTGAAGCCTTTACCAGATCATCTAAAGTATGTGTATCTTGGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTGAGACGTTGCCCATTATTGTTGCATCAGATTTAATACCGGAGCATGAGGAGGCCTTAATAAAATTGCTGCAGCAATACCGCAAAGCTATAGGTTGGACATTAGCTGATATTCAGGGAATTAGCCTATCTTTTTGTATGCACAAAATCACTCTAGAGGAGGGATCCTTTAGGAGTATTGAGCAACAAAGAAGGCTTAACCCTGCAATGAAAGAGGTTGTTAAAAAGGAGGTAATTAAATGGTTGGATGTTGGGATCATTTATCCAATTGCCGATAGCAATTGGGTAAGCCCTGTCCAATGTGTTCCTAAGAAAGGAGGTGTCACTGTGGTGAGCAATAAAGATAATGAGTTGATCCCCACAAGGACAGTAACTGGCTGGAGGGTTTGTATGGATTACAGAAGGCTTAATAAAGCCACTCGAAAGGATCATTTCCCTCTACCATTTATCGACAAGATGTTGGATCGATTGGCTGGTCAGGCCTATTACTGTTTCTTGGATGGTTATTCTGGGTATAACCAGATTACTATTGCTCCTGAAGATCGGGAAAAAACCACTTTCACCTGCCCTTATGGGACATTCGCTTTTAGGAGAATGCCTTTTGGCCTTTTGGCCTTTGCAATGCTCCAACAACATTTCAGTGGTGTATGTTAG

mRNA sequence

ATGAGCGATCCGCCTGGGGTACGGTTTGAGCTTGATCCAGAAATTGAAAGGACATTTAGGAACAGAAGGAGGGAGCAGCGTAGAAACCAGATGGAGAACGCCCCGCAACTTCCGCAGGACCAGAGCATTCGAGCATATGCTGTCCCGATGTTTAATGAGTTGAATCCAGGGATTGCACGTCCCCATATCCAAGCGGCAAATTTTGAAATGAAACCGGTAATGTTTCAGATGTTGCAAACCGTGGGGCAATTCCATGGTTTGTCATCTGAAGACCCTCATTTACATCTTAAGTCTTTTCTAGGAGTTAGTGATTCTTTTGTAATTCAAAGAGTGCCTAGAGATGCTCTTAGATTAACTTTGTTCCGTATTCTCTTAGAGATGGAGCAAAGTCATGTGATTAGTCATCAGCAGCCACCAGCTATGGAGCCTGCAGCAGTGGTGAACCAAGTCACGGACGAAGCATGTGTCTATTGCGGTGAAGACCACAACTACGAGTTTTGCCCCAGCAATCCAGCTTCTGTGTTGGCGCAACCACCCCAACTTCTCATGGGGAGTCAAGGAAATAACGTACAAGCGCAACAGAAGATGAACCAGCCGGGATTTGCTAAAGCGCAGCTAGCTAATGAGCTCAAGGCGAGGCCTCAAGGGAAACTTCCTTCGGATACTGAACACCCTCGAAGGGAAGGTAAGGAGCAGGTAAAGGCAGTAACTCTTAGGAGTGGTAAGCCACTAGAAGAGTCTAGAAAGACCCAGGATTTAAATAGTAATAGAAATAATAATGTTGTTATTGAGAAAGAGTTGGAGTCTGGACAGGGTGCTGGAGGCAGCAAAGAGAATGCTGGAGCATCTGGTTCTGTGCCAGATGTAGAAGCACCATATGTGCCGCCCCCACCTTATGTACCACCTCTACCTTTTCCACAAAGGCAAAAGCCTAAGAATAGGATGTGTGATTATCAAGAATGGTCTACCCCCAAGGCTAAGGATCCAGGGTCATTTACCATACCTGTGTCTATAGGTGGAAAGGAATTAGGTAGAGCACTCTGTGATTTAGGTGCAAGCATTAACCTTATGCCTCTTTCGGTCTATCGGAAGTTAGGTATTGGTGAAGCTAGTCCTACCATAGTTACACTCCAACTAGCTGATAGGTCTATCACATATCCAGAGGGTAAAATTGAGGATGTCTTAGTAAAAGTGGATAAATTCATATTTCCGGTTGATTTTATTATTTTAGACTATGAGGCTGATAAAGATGTCCCAATTATTCTAGGTCGTCCATTTTTGGCTACTGGTAGGGCATTAATAGATGTTCAAAAAGGAGAATTAACAATGAGAGTCTGTAATGAGGAAGTGAAATTTAATGTGTTTAAAGCCATGAAATATCCAGACGAAATGGAGGATTGCTCCTTCATTAGGATTCTGGAGAGCACAGTTATTGAGACAGCAATACAGGATTCGGCTGATAAGCATTCAGAAAAGCATGGAGAGCAATACCGCAAAGCTATAGGTTGGACATTAGCTGATATTCAGGGAATTAGCCTATCTTTTTGTATGCACAAAATCACTCTAGAGGAGGGATCCTTTAGGAGTATTGAGCAACAAAGAAGGCTTAACCCTGCAATGAAAGAGGTTGTTAAAAAGGAGGTAATTAAATGGTTGGATGTTGGGATCATTTATCCAATTGCCGATAGCAATTGGGTAAGCCCTGTCCAATGTGTTCCTAAGAAAGGAGGTGTCACTGTGGTGAGCAATAAAGATAATGAGTTGATCCCCACAAGGACAGTAACTGGCTGGAGGGTTTGTATGGATTACAGAAGGCTTAATAAAGCCACTCGAAAGGATCATTTCCCTCTACCATTTATCGACAAGATGTTGGATCGATTGGCTGGTCAGGCCTATTACTGTTTCTTGGATGGTTATTCTGGGTATAACCAGATTACTATTGCTCCTGAAGATCGGGAAAAAACCACTTTCACCTGCCCTTATGGGACATTCGCTTTTAGGAGAATGCCTTTTGGCCTTTTGGCCTTTGCAATGCTCCAACAACATTTCAGTGGTGTATGTTAG

Coding sequence (CDS)

ATGAGCGATCCGCCTGGGGTACGGTTTGAGCTTGATCCAGAAATTGAAAGGACATTTAGGAACAGAAGGAGGGAGCAGCGTAGAAACCAGATGGAGAACGCCCCGCAACTTCCGCAGGACCAGAGCATTCGAGCATATGCTGTCCCGATGTTTAATGAGTTGAATCCAGGGATTGCACGTCCCCATATCCAAGCGGCAAATTTTGAAATGAAACCGGTAATGTTTCAGATGTTGCAAACCGTGGGGCAATTCCATGGTTTGTCATCTGAAGACCCTCATTTACATCTTAAGTCTTTTCTAGGAGTTAGTGATTCTTTTGTAATTCAAAGAGTGCCTAGAGATGCTCTTAGATTAACTTTGTTCCGTATTCTCTTAGAGATGGAGCAAAGTCATGTGATTAGTCATCAGCAGCCACCAGCTATGGAGCCTGCAGCAGTGGTGAACCAAGTCACGGACGAAGCATGTGTCTATTGCGGTGAAGACCACAACTACGAGTTTTGCCCCAGCAATCCAGCTTCTGTGTTGGCGCAACCACCCCAACTTCTCATGGGGAGTCAAGGAAATAACGTACAAGCGCAACAGAAGATGAACCAGCCGGGATTTGCTAAAGCGCAGCTAGCTAATGAGCTCAAGGCGAGGCCTCAAGGGAAACTTCCTTCGGATACTGAACACCCTCGAAGGGAAGGTAAGGAGCAGGTAAAGGCAGTAACTCTTAGGAGTGGTAAGCCACTAGAAGAGTCTAGAAAGACCCAGGATTTAAATAGTAATAGAAATAATAATGTTGTTATTGAGAAAGAGTTGGAGTCTGGACAGGGTGCTGGAGGCAGCAAAGAGAATGCTGGAGCATCTGGTTCTGTGCCAGATGTAGAAGCACCATATGTGCCGCCCCCACCTTATGTACCACCTCTACCTTTTCCACAAAGGCAAAAGCCTAAGAATAGGATGTGTGATTATCAAGAATGGTCTACCCCCAAGGCTAAGGATCCAGGGTCATTTACCATACCTGTGTCTATAGGTGGAAAGGAATTAGGTAGAGCACTCTGTGATTTAGGTGCAAGCATTAACCTTATGCCTCTTTCGGTCTATCGGAAGTTAGGTATTGGTGAAGCTAGTCCTACCATAGTTACACTCCAACTAGCTGATAGGTCTATCACATATCCAGAGGGTAAAATTGAGGATGTCTTAGTAAAAGTGGATAAATTCATATTTCCGGTTGATTTTATTATTTTAGACTATGAGGCTGATAAAGATGTCCCAATTATTCTAGGTCGTCCATTTTTGGCTACTGGTAGGGCATTAATAGATGTTCAAAAAGGAGAATTAACAATGAGAGTCTGTAATGAGGAAGTGAAATTTAATGTGTTTAAAGCCATGAAATATCCAGACGAAATGGAGGATTGCTCCTTCATTAGGATTCTGGAGAGCACAGTTATTGAGACAGCAATACAGGATTCGGCTGATAAGCATTCAGAAAAGCATGGAGAGCAATACCGCAAAGCTATAGGTTGGACATTAGCTGATATTCAGGGAATTAGCCTATCTTTTTGTATGCACAAAATCACTCTAGAGGAGGGATCCTTTAGGAGTATTGAGCAACAAAGAAGGCTTAACCCTGCAATGAAAGAGGTTGTTAAAAAGGAGGTAATTAAATGGTTGGATGTTGGGATCATTTATCCAATTGCCGATAGCAATTGGGTAAGCCCTGTCCAATGTGTTCCTAAGAAAGGAGGTGTCACTGTGGTGAGCAATAAAGATAATGAGTTGATCCCCACAAGGACAGTAACTGGCTGGAGGGTTTGTATGGATTACAGAAGGCTTAATAAAGCCACTCGAAAGGATCATTTCCCTCTACCATTTATCGACAAGATGTTGGATCGATTGGCTGGTCAGGCCTATTACTGTTTCTTGGATGGTTATTCTGGGTATAACCAGATTACTATTGCTCCTGAAGATCGGGAAAAAACCACTTTCACCTGCCCTTATGGGACATTCGCTTTTAGGAGAATGCCTTTTGGCCTTTTGGCCTTTGCAATGCTCCAACAACATTTCAGTGGTGTATGTTAG

Protein sequence

MSDPPGVRFELDPEIERTFRNRRREQRRNQMENAPQLPQDQSIRAYAVPMFNELNPGIARPHIQAANFEMKPVMFQMLQTVGQFHGLSSEDPHLHLKSFLGVSDSFVIQRVPRDALRLTLFRILLEMEQSHVISHQQPPAMEPAAVVNQVTDEACVYCGEDHNYEFCPSNPASVLAQPPQLLMGSQGNNVQAQQKMNQPGFAKAQLANELKARPQGKLPSDTEHPRREGKEQVKAVTLRSGKPLEESRKTQDLNSNRNNNVVIEKELESGQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQRQKPKNRMCDYQEWSTPKAKDPGSFTIPVSIGGKELGRALCDLGASINLMPLSVYRKLGIGEASPTIVTLQLADRSITYPEGKIEDVLVKVDKFIFPVDFIILDYEADKDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFNVFKAMKYPDEMEDCSFIRILESTVIETAIQDSADKHSEKHGEQYRKAIGWTLADIQGISLSFCMHKITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSNWVSPVQCVPKKGGVTVVSNKDNELIPTRTVTGWRVCMDYRRLNKATRKDHFPLPFIDKMLDRLAGQAYYCFLDGYSGYNQITIAPEDREKTTFTCPYGTFAFRRMPFGLLAFAMLQQHFSGVC
Homology
BLAST of Lag0015918 vs. NCBI nr
Match: PIN12235.1 (DNA-directed DNA polymerase [Handroanthus impetiginosus])

HSP 1 Score: 540.0 bits (1390), Expect = 2.9e-149
Identity = 322/649 (49.61%), Postives = 389/649 (59.94%), Query Frame = 0

Query: 147 VNQV--TDEACVYCGEDHNYEFCPSNPASVL----AQPPQLLMGSQGNNVQAQQKMN--- 206
           VNQV  T   C  CGE H  + CP +  S+     A+ PQ    S   N   +Q  N   
Sbjct: 107 VNQVQHTPVTCDECGESHPSDQCPHSVESIQFVSNARKPQNNPYSNTYNPGWRQHPNFSW 166

Query: 207 --------QPGFAKAQLANELKARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESR 266
                    P F + QLAN + +RPQG L S+TE +PR++GK Q +AVTLR+G+ L+E  
Sbjct: 167 NNNQGQGSAPRFQQGQLANAINSRPQGSLLSNTEPNPRQDGKAQCQAVTLRNGRELQEVV 226

Query: 267 KTQDLNSNRNNNVVIEKELESGQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR 326
           K    +  +    VI +E E    A   K +     ++P  EA     P YV  +     
Sbjct: 227 KEPTKSKGKE---VIPEEKEKEVEALLEKLHI----NIPFAEA-LEQMPSYVKFM--KDI 286

Query: 327 QKPKNRMCDY-------------QEWSTPKAKDPGSFTIPVSIGGKELGRALCDLGASIN 386
              K R+ DY             Q    PK KDPGSFTIP +IG    GRALCDLGASIN
Sbjct: 287 LSKKRRLGDYEMVALTEECSAIIQNKLPPKLKDPGSFTIPCTIGTHFSGRALCDLGASIN 346

Query: 387 LMPLSVYRKLGIGEASPTIVTLQLADRSITYPEGKIEDVLVKVDKFIFPVDFIILDYEAD 446
           LMP S+YR LG+GEA  T +TLQLADRS+TYP+G IED+LVKVDKFIFP DF++LD + D
Sbjct: 347 LMPYSIYRTLGLGEAKSTSITLQLADRSLTYPKGVIEDILVKVDKFIFPADFVVLDMKVD 406

Query: 447 KDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFNVFKAMKYPDEMEDCSFIRILES 506
            +VPIILGRPFLATGR LIDVQKGELTMRV ++++ FNVFKAMK+P+E ++C  + + ++
Sbjct: 407 SEVPIILGRPFLATGRTLIDVQKGELTMRVQDQQITFNVFKAMKFPNESDECFSVSLFDN 466

Query: 507 TV------------IETAIQDSADKHSEKHGE---------------------------- 566
                         +E A+ D  D+ +E+  E                            
Sbjct: 467 LAGNESIAEQPLDPLERALLDLIDEDNEEDLEVVQTLDASKFFKSRGVESLERTAPSKVL 526

Query: 567 ---------------------------------------------------QYRKAIGWT 626
                                                               ++ AIGWT
Sbjct: 527 KPSIEDPPTLELKPLPSHLCYAYLGEYDTLPVIISSSLSDVQVEKLLRVIRDHKCAIGWT 586

Query: 627 LADIQGISLSFCMHKITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSN 674
           +ADI+GIS SFCMHKI LE+    S+E QRRLNP MKEVVKKE+IKWLD GIIYPI+DS+
Sbjct: 587 IADIKGISPSFCMHKILLEDDQKPSVESQRRLNPIMKEVVKKEIIKWLDAGIIYPISDSS 646

BLAST of Lag0015918 vs. NCBI nr
Match: XP_023522102.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111785979 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 539.7 bits (1389), Expect = 3.8e-149
Identity = 318/692 (45.95%), Postives = 394/692 (56.94%), Query Frame = 0

Query: 144 AAVVNQVTDEACVYCGEDHNYEFCPSNPASVLAQPPQLLMGSQGNNVQAQQKMNQPGFAK 203
           AAV+NQ   E+C         E+   N A++ +Q   L       N++ Q          
Sbjct: 98  AAVINQTAAESCSLI-----KEYMAKNDAAIQSQQASL------RNLEVQ---------V 157

Query: 204 AQLANELKARPQGKLPSDTEHPRREGKEQVKAVTLRSGKPL------------EESRKTQ 263
            QLANEL+ RP  KLP+DTE P+REG EQ +A+ LRSGK +              S++T 
Sbjct: 158 GQLANELRNRPLSKLPADTEMPKREGMEQCQAIELRSGKEIPSRGEKIKEHGDSRSQETA 217

Query: 264 DLNSNRNNNVVIEKELESGQGAGGSKENAGASGSVPDVEAPYVPPPP------------- 323
           D    +   VV E+  ++   A   KE++     +       V PP              
Sbjct: 218 DTQQRKEKAVVQEEHNKNDAEAAVQKEHSRNYAEI-------VKPPKTQTSVSSGQESRI 277

Query: 324 YVPPLPFPQRQKPKNRMCDYQEW---------STP------------------------- 383
           Y P  PFPQR K K     ++++         + P                         
Sbjct: 278 YTPTPPFPQRIKRKKEEAHFEKFMDIFKEIHINIPLVEALKQMPNYVKFLKDVLTNRRKF 337

Query: 384 ----------------------KAKDPGSFTIPVSIGGKELGRALCDLGASINLMPLSVY 443
                                 K KDPGSFTIP+SIGGK+LGRALCDLG+SINLMPLS+Y
Sbjct: 338 EEFKVVPLNEECSAILKNKIPLKEKDPGSFTIPISIGGKKLGRALCDLGSSINLMPLSIY 397

Query: 444 RKLGIGEASPTIVTLQLADRSITYPEGKIEDVLVKVDKFIFPVDFIILDYEADKDVPIIL 503
           +KLGIGEA PT VTLQLADRS TYPEGKIED+L++VDKFIFP DFIILDYEAD DVPIIL
Sbjct: 398 KKLGIGEARPTTVTLQLADRSFTYPEGKIEDILIQVDKFIFPADFIILDYEADHDVPIIL 457

Query: 504 GRPFLATGRALIDVQKGELTMRVCNEEVKFNVFKAMKYPDEMEDCS-------------- 563
           GRPFL TGR L+DV KG +T+R+ +++V+FN+  +MKYP   E+CS              
Sbjct: 458 GRPFLKTGRTLVDVYKGTITLRMGDQKVEFNINDSMKYPAVTEECSAVYELTEQPATEEW 517

Query: 564 -------------------------FIRILES---------------------------- 623
                                    F R  ES                            
Sbjct: 518 DDGESGQEEDSSWNDRIEQLAVLGEFSRTFESLECEGRKSSPMRPSIEEAPQLDLKPLPP 577

Query: 624 --------------TVIETAIQDSADKHSEKHGEQYRKAIGWTLADIQGISLSFCMHKIT 674
                          +I   +  + +    +  ++++ AIGWTLADI+GIS S CMHKI 
Sbjct: 578 NLKYAYLGDKKTLPIIISATLSSTQEDMLLETLKKHKGAIGWTLADIKGISPSLCMHKIQ 637

BLAST of Lag0015918 vs. NCBI nr
Match: PIN22487.1 (DNA-directed DNA polymerase [Handroanthus impetiginosus])

HSP 1 Score: 537.0 bits (1382), Expect = 2.5e-148
Identity = 333/731 (45.55%), Postives = 395/731 (54.04%), Query Frame = 0

Query: 147 VNQV--TDEACVYCGEDHNYEFCPSNPASVL----AQPPQ-------------------- 206
           VNQV  T   C  CGE H  + CP +  S+     A+ PQ                    
Sbjct: 119 VNQVQHTPVTCEECGEGHPSDQCPHSVESIQFVSNARKPQNNPYSNTYNPGWRQHPNFSW 178

Query: 207 ---LLMGS-----QGNNVQAQQKMNQ--PGFAK--------------------AQLANEL 266
                 GS     QG   Q QQ M +  P   +                     QLAN +
Sbjct: 179 NNNQGQGSAPRFQQGGQQQVQQPMQEKKPSLEETLIQFMASTAANFKTMETQIGQLANAI 238

Query: 267 KARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESRKTQDLNSNRNNNVVIEKELES 326
            +RPQG LPS+TE +PR++GK Q +AVTLR+G+ L+E  K    +  +    VI +E E 
Sbjct: 239 NSRPQGSLPSNTEPNPRQDGKAQCQAVTLRNGRELQEVVKEPTKSKEKE---VISEEKEK 298

Query: 327 GQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR--------------------- 386
                             +VEAP     P     PFPQR                     
Sbjct: 299 ------------------EVEAPLEVSKPTTLQPPFPQRLQKQKLEKQFLKFLEVFKKLH 358

Query: 387 ----------QKP------------KNRMCDY-------------QEWSTPKAKDPGSFT 446
                     Q P            K R+ DY             Q    PK KDPGSFT
Sbjct: 359 INIPFAEALEQMPSYVKFMKDILSKKRRLGDYETVALTEECSAIIQNKLPPKLKDPGSFT 418

Query: 447 IPVSIGGKELGRALCDLGASINLMPLSVYRKLGIGEASPTIVTLQLADRSITYPEGKIED 506
           IP +IG    GRALCDLGASINLMP S+YR LG+GEA PT +TLQLADRS+TYP+G IED
Sbjct: 419 IPCTIGTHFSGRALCDLGASINLMPYSIYRTLGLGEAKPTSITLQLADRSLTYPKGVIED 478

Query: 507 VLVKVDKFIFPVDFIILDYEADKDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFN 566
           +LVKVDKFIFP DF++LD E D +VPIILGRPFLATGR LIDVQKGELTMRV ++++ FN
Sbjct: 479 ILVKVDKFIFPADFVVLDMEVDSEVPIILGRPFLATGRTLIDVQKGELTMRVQDQQITFN 538

Query: 567 VFKAMKYPDEMEDCSFIRILESTV------------IETAIQDSADKHSEKHGE------ 626
           VFKAMK+P+E ++C  + + +               +E A+ D  D+ +E+  E      
Sbjct: 539 VFKAMKFPNESDECFAVSLFDKLAGNESIAEPPLDPLERALLDLLDEENEEDLEVVKTLD 598

Query: 627 ------------------------------------------------------------ 674
                                                                       
Sbjct: 599 ASKFLKSRRVESLERTTPSKVLKPSIEDPPTLELKPLPSHLCYAYLGESDTLPVIISSSL 658

BLAST of Lag0015918 vs. NCBI nr
Match: PIN22518.1 (DNA-directed DNA polymerase [Handroanthus impetiginosus])

HSP 1 Score: 534.3 bits (1375), Expect = 1.6e-147
Identity = 306/617 (49.59%), Postives = 364/617 (59.00%), Query Frame = 0

Query: 205 QLANELKARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESRKTQDLNSNRNNNVVI 264
           QLAN + +RPQG LPS+TE +PR++GK Q +AVTLR+G+ L+E  K    +  +    VI
Sbjct: 217 QLANAINSRPQGSLPSNTEPNPRQDGKAQCQAVTLRNGRKLQEVVKKPTKSKEKE---VI 276

Query: 265 EKELESGQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR--------------- 324
            KE E                   +VEAP     P     PFPQ+               
Sbjct: 277 SKEKEK------------------EVEAPLEVSKPTTLQPPFPQKLQKQKLEKQFLKFLE 336

Query: 325 ----------------QKP------------KNRMCDY-------------QEWSTPKAK 384
                           Q P            K R+ DY             Q    PK K
Sbjct: 337 VFKKLHINIPFAEALEQMPSYVKFMKDILSKKRRLGDYETAALTEECNAIIQNKLPPKLK 396

Query: 385 DPGSFTIPVSIGGKELGRALCDLGASINLMPLSVYRKLGIGEASPTIVTLQLADRSITYP 444
           DPGSFTIP +IG    GRALCDLGASINLMP S+YR LG+GEA PT +TLQLADRS+TYP
Sbjct: 397 DPGSFTIPCTIGTHFSGRALCDLGASINLMPYSIYRTLGLGEAKPTSITLQLADRSLTYP 456

Query: 445 EGKIEDVLVKVDKFIFPVDFIILDYEADKDVPIILGRPFLATGRALIDVQKGELTMRVCN 504
           +G IED+LVKVDKFIFP DF++LD E D +VPIILGRPFLATGR LIDVQKGELTMRV +
Sbjct: 457 KGVIEDILVKVDKFIFPADFVVLDMEVDSEVPIILGRPFLATGRTLIDVQKGELTMRVQD 516

Query: 505 EEVKFNVFKAMKYPDEMEDCSFIRILESTV------------IETAIQDSADKHSEKHGE 564
           +++ FNVFKAMK+P+E ++C  + + ++              +E A+ D  ++ +E+  E
Sbjct: 517 QQITFNVFKAMKFPNESDECFSVSLFDNLAGNESIAEQPLDSLERALLDLIEEGNEEDLE 576

Query: 565 ------------------------------------------------------------ 624
                                                                       
Sbjct: 577 VVKTLNASKFFKSRGVESLERTTPSKVLKPSIEDPPTLELKPLPNHLCYVYLGESDTLPV 636

Query: 625 -------------------QYRKAIGWTLADIQGISLSFCMHKITLEEGSFRSIEQQRRL 674
                               ++ AIGWT+ADI+GIS SFCMHKI LE+    S+E QRRL
Sbjct: 637 IISSSLSDLQVEKLLRVLRNHKGAIGWTIADIKGISPSFCMHKILLEDDQKPSVESQRRL 696

BLAST of Lag0015918 vs. NCBI nr
Match: PIN00194.1 (DNA-directed DNA polymerase [Handroanthus impetiginosus])

HSP 1 Score: 531.6 bits (1368), Expect = 1.0e-146
Identity = 318/649 (49.00%), Postives = 387/649 (59.63%), Query Frame = 0

Query: 147 VNQV--TDEACVYCGEDHNYEFCPSNPASVL----AQPPQLLMGSQGNNVQAQQKMN--- 206
           VNQV  T   C  CGE H  + CP +  S+     A+ PQ    S   N   +Q  N   
Sbjct: 9   VNQVQHTPVTCKECGEGHPSDQCPHSVESIQFVSNARKPQNNPYSNTYNSGWRQHPNFSW 68

Query: 207 --------QPGFAKAQLANELKARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESR 266
                    P F + QLAN + +RPQG LPS+TE +PR+  K Q +AVTLR+G+ L+E  
Sbjct: 69  NNNQGQGSAPRFQQGQLANAINSRPQGSLPSNTEPNPRQNDKAQCQAVTLRNGRELQEVV 128

Query: 267 KTQDLNSNRNNNVVIEKELESGQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR 326
           K    +  +    VI +E E    A   K +     ++P  EA     P YV  +     
Sbjct: 129 KEPTKSKEKE---VISEEKEKEVEAPLEKLHI----NIPFAEA-LEQMPSYVKFI--EDI 188

Query: 327 QKPKNRMCDY-------------QEWSTPKAKDPGSFTIPVSIGGKELGRALCDLGASIN 386
              K R+ DY             Q    PK KDPGSFTIP +IG    GRALCDLGASIN
Sbjct: 189 LSNKRRLGDYETVALTEECSAIIQNKLPPKLKDPGSFTIPCTIGTHFSGRALCDLGASIN 248

Query: 387 LMPLSVYRKLGIGEASPTIVTLQLADRSITYPEGKIEDVLVKVDKFIFPVDFIILDYEAD 446
           LMP S+YR LG+G+A PT +TLQLADRS+TYP+G IED+LVKVDKFIFP  F++LD E D
Sbjct: 249 LMPYSIYRTLGLGKAKPTSITLQLADRSLTYPKGVIEDILVKVDKFIFPAYFVVLDMEVD 308

Query: 447 KDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFNVFKAMKYPDEMEDCSFIRILES 506
            +VPIILGRPFLATGR LI+VQKGELTMRV ++++ FNVFKAMK+ +E ++C  + + ++
Sbjct: 309 IEVPIILGRPFLATGRTLINVQKGELTMRVQDQQITFNVFKAMKFSNESDECFDVSLFDN 368

Query: 507 TV------------IETAIQDSADKHSEKHGE---------------------------- 566
                         +E A+ D  D+ +E+  E                            
Sbjct: 369 LAGDESIAEQPLDPLERALLDLMDEENEEDLEVVKTLDASKYFKSRGVESLERTAPSKVL 428

Query: 567 ---------------------------------------------------QYRKAIGWT 626
                                                               ++ AIGWT
Sbjct: 429 KPSIEEPPTLELKPLPSHLCYAYLGESDTLPVIISSSFSDLQVENFLRVLRNHKCAIGWT 488

Query: 627 LADIQGISLSFCMHKITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSN 674
           +ADI+GIS SFCMHKI LE+    S++ QRRLNP MKEVVKKE+IKWLDVGIIYPI+DS+
Sbjct: 489 IADIKGISPSFCMHKILLEDDQKPSVKSQRRLNPIMKEVVKKEIIKWLDVGIIYPISDSS 548

BLAST of Lag0015918 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-20
Identity = 74/215 (34.42%), Postives = 104/215 (48.37%), Query Frame = 0

Query: 475 STVIET--AIQDSADKHSEKH---------GEQYRKAIGWTL----ADIQGISLSFCMHK 534
           S V+ T  +++ +A  HS K           ++YR+ I   L    ADI  I +    H 
Sbjct: 531 SNVVSTIQSVEPNATDHSNKDTFCTLPVWLQQKYREIIRNDLPPRPADINNIPVK---HD 590

Query: 535 ITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSNWVSPVQCVPKKGGVT 594
           I ++ G+     Q   +    ++ + K V K LD   I P + S   SPV  VPKK G  
Sbjct: 591 IEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVP-SKSPCSSPVVLVPKKDGT- 650

Query: 595 VVSNKDNELIPTRTVTGWRVCMDYRRLNKATRKDHFPLPFIDKMLDRLAGQAYYCFLDGY 654
                            +R+C+DYR LNKAT  D FPLP ID +L R+     +  LD +
Sbjct: 651 -----------------FRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLH 710

Query: 655 SGYNQITIAPEDREKTTFTCPYGTFAFRRMPFGLL 675
           SGY+QI + P+DR KT F  P G + +  MPFGL+
Sbjct: 711 SGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLV 723

BLAST of Lag0015918 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-20
Identity = 74/215 (34.42%), Postives = 104/215 (48.37%), Query Frame = 0

Query: 475 STVIET--AIQDSADKHSEKH---------GEQYRKAIGWTL----ADIQGISLSFCMHK 534
           S V+ T  +++ +A  HS K           ++YR+ I   L    ADI  I +    H 
Sbjct: 557 SNVVSTIQSVEPNATDHSNKDTFCTLPVWLQQKYREIIRNDLPPRPADINNIPVK---HD 616

Query: 535 ITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSNWVSPVQCVPKKGGVT 594
           I ++ G+     Q   +    ++ + K V K LD   I P + S   SPV  VPKK G  
Sbjct: 617 IEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVP-SKSPCSSPVVLVPKKDGT- 676

Query: 595 VVSNKDNELIPTRTVTGWRVCMDYRRLNKATRKDHFPLPFIDKMLDRLAGQAYYCFLDGY 654
                            +R+C+DYR LNKAT  D FPLP ID +L R+     +  LD +
Sbjct: 677 -----------------FRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLH 736

Query: 655 SGYNQITIAPEDREKTTFTCPYGTFAFRRMPFGLL 675
           SGY+QI + P+DR KT F  P G + +  MPFGL+
Sbjct: 737 SGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLV 749

BLAST of Lag0015918 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.3e-16
Identity = 85/341 (24.93%), Postives = 153/341 (44.87%), Query Frame = 0

Query: 345 RALCDLGASINLMPLSVYRKLGIGEASPTIVTLQ---LADRSITYPEGKIEDVLVKVDKF 404
           + L D G+++N+   +++  L I   S  I T     + ++SI  P            K 
Sbjct: 26  KCLIDTGSTVNMTSKNIF-DLPIQNTSTFIHTSNGPLIVNKSIIIP-----------SKI 85

Query: 405 IFPVDFIILDYEADKDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFNVFKAMKYP 464
           +FP     L +   ++  ++LGR  LA  +A I  +  E+T+   N + K     A    
Sbjct: 86  LFPTTNEFLLHPFSENYDLLLGRKLLAEAKATISYRDQEVTL--YNNKYKLIEGIATHEQ 145

Query: 465 DEMEDCSFI---RILESTVIETAIQDSADKHSEKHGEQYRK--AIGWTLADIQ---GISL 524
              ++ + I    + +   I   ++    +    + E+ ++  A+     DIQ   G  L
Sbjct: 146 SHFQNVNMIPDTMLRQPNKISPILESDLYRLEHLNNEEKQRLCALLQKYHDIQYHEGDKL 205

Query: 525 SFC-MHKITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSNWVSPVQCV 584
           +F    K T+       +  +     A ++ V+ ++   L+ GII   ++S + SP+  V
Sbjct: 206 TFTNQTKHTINTKHNLPLYSKYSYPQAYEQEVESQIQDMLNQGII-RTSNSPYNSPIWVV 265

Query: 585 PKKGGVTVVSNKDNELIPTRTVTGWRVCMDYRRLNKATRKDHFPLPFIDKMLDRLAGQAY 644
           PKK      S K            +R+ +DYR+LN+ T  D  P+P +D++L +L    Y
Sbjct: 266 PKKQD---ASGKQK----------FRIVIDYRKLNEITVGDRHPIPNMDEILGKLGRCNY 325

Query: 645 YCFLDGYSGYNQITIAPEDREKTTFTCPYGTFAFRRMPFGL 674
           +  +D   G++QI + PE   KT F+  +G + + RMPFGL
Sbjct: 326 FTTIDLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGL 338

BLAST of Lag0015918 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 9.7e-15
Identity = 47/132 (35.61%), Postives = 71/132 (53.79%), Query Frame = 0

Query: 542 EVVKKEVIKWLDVGIIYPIADSNWVSPVQCVPKKGGVTVVSNKDNELIPTRTVTGWRVCM 601
           E ++ +V K +   I+ P + S + SP+  VPKK              P      WR+ +
Sbjct: 328 EEIQAQVQKLIKDKIVEP-SVSQYNSPLLLVPKKSS------------PNSDKKKWRLVI 387

Query: 602 DYRRLNKATRKDHFPLPFIDKMLDRLAGQAYYCFLDGYSGYNQITIAPEDREKTTFTCPY 661
           DYR++NK    D FPLP ID +LD+L    Y+  LD  SG++QI +    R+ T+F+   
Sbjct: 388 DYRQINKKLLADKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSFSTSN 446

Query: 662 GTFAFRRMPFGL 674
           G++ F R+PFGL
Sbjct: 448 GSYRFTRLPFGL 446

BLAST of Lag0015918 vs. ExPASy Swiss-Prot
Match: P31843 (RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 4.8e-14
Identity = 38/76 (50.00%), Postives = 49/76 (64.47%), Query Frame = 0

Query: 598 RVCMDYRRLNKATRKDHFPLPFIDKMLDRLAGQAYYCFLDGYSGYNQITIAPEDREKTTF 657
           R+C+DYR L K T K+ +P+P +D + DRLA   ++  LD  SGY Q+ IA  D  KTT 
Sbjct: 7   RMCIDYRALTKVTIKNKYPIPRVDDLFDRLAQATWFTKLDLRSGYWQVRIAKGDEPKTTC 66

Query: 658 TCPYGTFAFRRMPFGL 674
              YG+F FR MPFGL
Sbjct: 67  VTRYGSFEFRVMPFGL 82

BLAST of Lag0015918 vs. ExPASy TrEMBL
Match: A0A2G9H400 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_15163 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 1.4e-149
Identity = 322/649 (49.61%), Postives = 389/649 (59.94%), Query Frame = 0

Query: 147 VNQV--TDEACVYCGEDHNYEFCPSNPASVL----AQPPQLLMGSQGNNVQAQQKMN--- 206
           VNQV  T   C  CGE H  + CP +  S+     A+ PQ    S   N   +Q  N   
Sbjct: 107 VNQVQHTPVTCDECGESHPSDQCPHSVESIQFVSNARKPQNNPYSNTYNPGWRQHPNFSW 166

Query: 207 --------QPGFAKAQLANELKARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESR 266
                    P F + QLAN + +RPQG L S+TE +PR++GK Q +AVTLR+G+ L+E  
Sbjct: 167 NNNQGQGSAPRFQQGQLANAINSRPQGSLLSNTEPNPRQDGKAQCQAVTLRNGRELQEVV 226

Query: 267 KTQDLNSNRNNNVVIEKELESGQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR 326
           K    +  +    VI +E E    A   K +     ++P  EA     P YV  +     
Sbjct: 227 KEPTKSKGKE---VIPEEKEKEVEALLEKLHI----NIPFAEA-LEQMPSYVKFM--KDI 286

Query: 327 QKPKNRMCDY-------------QEWSTPKAKDPGSFTIPVSIGGKELGRALCDLGASIN 386
              K R+ DY             Q    PK KDPGSFTIP +IG    GRALCDLGASIN
Sbjct: 287 LSKKRRLGDYEMVALTEECSAIIQNKLPPKLKDPGSFTIPCTIGTHFSGRALCDLGASIN 346

Query: 387 LMPLSVYRKLGIGEASPTIVTLQLADRSITYPEGKIEDVLVKVDKFIFPVDFIILDYEAD 446
           LMP S+YR LG+GEA  T +TLQLADRS+TYP+G IED+LVKVDKFIFP DF++LD + D
Sbjct: 347 LMPYSIYRTLGLGEAKSTSITLQLADRSLTYPKGVIEDILVKVDKFIFPADFVVLDMKVD 406

Query: 447 KDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFNVFKAMKYPDEMEDCSFIRILES 506
            +VPIILGRPFLATGR LIDVQKGELTMRV ++++ FNVFKAMK+P+E ++C  + + ++
Sbjct: 407 SEVPIILGRPFLATGRTLIDVQKGELTMRVQDQQITFNVFKAMKFPNESDECFSVSLFDN 466

Query: 507 TV------------IETAIQDSADKHSEKHGE---------------------------- 566
                         +E A+ D  D+ +E+  E                            
Sbjct: 467 LAGNESIAEQPLDPLERALLDLIDEDNEEDLEVVQTLDASKFFKSRGVESLERTAPSKVL 526

Query: 567 ---------------------------------------------------QYRKAIGWT 626
                                                               ++ AIGWT
Sbjct: 527 KPSIEDPPTLELKPLPSHLCYAYLGEYDTLPVIISSSLSDVQVEKLLRVIRDHKCAIGWT 586

Query: 627 LADIQGISLSFCMHKITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSN 674
           +ADI+GIS SFCMHKI LE+    S+E QRRLNP MKEVVKKE+IKWLD GIIYPI+DS+
Sbjct: 587 IADIKGISPSFCMHKILLEDDQKPSVESQRRLNPIMKEVVKKEIIKWLDAGIIYPISDSS 646

BLAST of Lag0015918 vs. ExPASy TrEMBL
Match: A0A2G9HYA0 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04802 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 1.2e-148
Identity = 333/731 (45.55%), Postives = 395/731 (54.04%), Query Frame = 0

Query: 147 VNQV--TDEACVYCGEDHNYEFCPSNPASVL----AQPPQ-------------------- 206
           VNQV  T   C  CGE H  + CP +  S+     A+ PQ                    
Sbjct: 119 VNQVQHTPVTCEECGEGHPSDQCPHSVESIQFVSNARKPQNNPYSNTYNPGWRQHPNFSW 178

Query: 207 ---LLMGS-----QGNNVQAQQKMNQ--PGFAK--------------------AQLANEL 266
                 GS     QG   Q QQ M +  P   +                     QLAN +
Sbjct: 179 NNNQGQGSAPRFQQGGQQQVQQPMQEKKPSLEETLIQFMASTAANFKTMETQIGQLANAI 238

Query: 267 KARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESRKTQDLNSNRNNNVVIEKELES 326
            +RPQG LPS+TE +PR++GK Q +AVTLR+G+ L+E  K    +  +    VI +E E 
Sbjct: 239 NSRPQGSLPSNTEPNPRQDGKAQCQAVTLRNGRELQEVVKEPTKSKEKE---VISEEKEK 298

Query: 327 GQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR--------------------- 386
                             +VEAP     P     PFPQR                     
Sbjct: 299 ------------------EVEAPLEVSKPTTLQPPFPQRLQKQKLEKQFLKFLEVFKKLH 358

Query: 387 ----------QKP------------KNRMCDY-------------QEWSTPKAKDPGSFT 446
                     Q P            K R+ DY             Q    PK KDPGSFT
Sbjct: 359 INIPFAEALEQMPSYVKFMKDILSKKRRLGDYETVALTEECSAIIQNKLPPKLKDPGSFT 418

Query: 447 IPVSIGGKELGRALCDLGASINLMPLSVYRKLGIGEASPTIVTLQLADRSITYPEGKIED 506
           IP +IG    GRALCDLGASINLMP S+YR LG+GEA PT +TLQLADRS+TYP+G IED
Sbjct: 419 IPCTIGTHFSGRALCDLGASINLMPYSIYRTLGLGEAKPTSITLQLADRSLTYPKGVIED 478

Query: 507 VLVKVDKFIFPVDFIILDYEADKDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFN 566
           +LVKVDKFIFP DF++LD E D +VPIILGRPFLATGR LIDVQKGELTMRV ++++ FN
Sbjct: 479 ILVKVDKFIFPADFVVLDMEVDSEVPIILGRPFLATGRTLIDVQKGELTMRVQDQQITFN 538

Query: 567 VFKAMKYPDEMEDCSFIRILESTV------------IETAIQDSADKHSEKHGE------ 626
           VFKAMK+P+E ++C  + + +               +E A+ D  D+ +E+  E      
Sbjct: 539 VFKAMKFPNESDECFAVSLFDKLAGNESIAEPPLDPLERALLDLLDEENEEDLEVVKTLD 598

Query: 627 ------------------------------------------------------------ 674
                                                                       
Sbjct: 599 ASKFLKSRRVESLERTTPSKVLKPSIEDPPTLELKPLPSHLCYAYLGESDTLPVIISSSL 658

BLAST of Lag0015918 vs. ExPASy TrEMBL
Match: A0A2G9HYD8 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04775 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 7.7e-148
Identity = 306/617 (49.59%), Postives = 364/617 (59.00%), Query Frame = 0

Query: 205 QLANELKARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESRKTQDLNSNRNNNVVI 264
           QLAN + +RPQG LPS+TE +PR++GK Q +AVTLR+G+ L+E  K    +  +    VI
Sbjct: 217 QLANAINSRPQGSLPSNTEPNPRQDGKAQCQAVTLRNGRKLQEVVKKPTKSKEKE---VI 276

Query: 265 EKELESGQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR--------------- 324
            KE E                   +VEAP     P     PFPQ+               
Sbjct: 277 SKEKEK------------------EVEAPLEVSKPTTLQPPFPQKLQKQKLEKQFLKFLE 336

Query: 325 ----------------QKP------------KNRMCDY-------------QEWSTPKAK 384
                           Q P            K R+ DY             Q    PK K
Sbjct: 337 VFKKLHINIPFAEALEQMPSYVKFMKDILSKKRRLGDYETAALTEECNAIIQNKLPPKLK 396

Query: 385 DPGSFTIPVSIGGKELGRALCDLGASINLMPLSVYRKLGIGEASPTIVTLQLADRSITYP 444
           DPGSFTIP +IG    GRALCDLGASINLMP S+YR LG+GEA PT +TLQLADRS+TYP
Sbjct: 397 DPGSFTIPCTIGTHFSGRALCDLGASINLMPYSIYRTLGLGEAKPTSITLQLADRSLTYP 456

Query: 445 EGKIEDVLVKVDKFIFPVDFIILDYEADKDVPIILGRPFLATGRALIDVQKGELTMRVCN 504
           +G IED+LVKVDKFIFP DF++LD E D +VPIILGRPFLATGR LIDVQKGELTMRV +
Sbjct: 457 KGVIEDILVKVDKFIFPADFVVLDMEVDSEVPIILGRPFLATGRTLIDVQKGELTMRVQD 516

Query: 505 EEVKFNVFKAMKYPDEMEDCSFIRILESTV------------IETAIQDSADKHSEKHGE 564
           +++ FNVFKAMK+P+E ++C  + + ++              +E A+ D  ++ +E+  E
Sbjct: 517 QQITFNVFKAMKFPNESDECFSVSLFDNLAGNESIAEQPLDSLERALLDLIEEGNEEDLE 576

Query: 565 ------------------------------------------------------------ 624
                                                                       
Sbjct: 577 VVKTLNASKFFKSRGVESLERTTPSKVLKPSIEDPPTLELKPLPNHLCYVYLGESDTLPV 636

Query: 625 -------------------QYRKAIGWTLADIQGISLSFCMHKITLEEGSFRSIEQQRRL 674
                               ++ AIGWT+ADI+GIS SFCMHKI LE+    S+E QRRL
Sbjct: 637 IISSSLSDLQVEKLLRVLRNHKGAIGWTIADIKGISPSFCMHKILLEDDQKPSVESQRRL 696

BLAST of Lag0015918 vs. ExPASy TrEMBL
Match: A0A2G9G4F1 (DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_27304 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 5.0e-147
Identity = 318/649 (49.00%), Postives = 387/649 (59.63%), Query Frame = 0

Query: 147 VNQV--TDEACVYCGEDHNYEFCPSNPASVL----AQPPQLLMGSQGNNVQAQQKMN--- 206
           VNQV  T   C  CGE H  + CP +  S+     A+ PQ    S   N   +Q  N   
Sbjct: 9   VNQVQHTPVTCKECGEGHPSDQCPHSVESIQFVSNARKPQNNPYSNTYNSGWRQHPNFSW 68

Query: 207 --------QPGFAKAQLANELKARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESR 266
                    P F + QLAN + +RPQG LPS+TE +PR+  K Q +AVTLR+G+ L+E  
Sbjct: 69  NNNQGQGSAPRFQQGQLANAINSRPQGSLPSNTEPNPRQNDKAQCQAVTLRNGRELQEVV 128

Query: 267 KTQDLNSNRNNNVVIEKELESGQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQR 326
           K    +  +    VI +E E    A   K +     ++P  EA     P YV  +     
Sbjct: 129 KEPTKSKEKE---VISEEKEKEVEAPLEKLHI----NIPFAEA-LEQMPSYVKFI--EDI 188

Query: 327 QKPKNRMCDY-------------QEWSTPKAKDPGSFTIPVSIGGKELGRALCDLGASIN 386
              K R+ DY             Q    PK KDPGSFTIP +IG    GRALCDLGASIN
Sbjct: 189 LSNKRRLGDYETVALTEECSAIIQNKLPPKLKDPGSFTIPCTIGTHFSGRALCDLGASIN 248

Query: 387 LMPLSVYRKLGIGEASPTIVTLQLADRSITYPEGKIEDVLVKVDKFIFPVDFIILDYEAD 446
           LMP S+YR LG+G+A PT +TLQLADRS+TYP+G IED+LVKVDKFIFP  F++LD E D
Sbjct: 249 LMPYSIYRTLGLGKAKPTSITLQLADRSLTYPKGVIEDILVKVDKFIFPAYFVVLDMEVD 308

Query: 447 KDVPIILGRPFLATGRALIDVQKGELTMRVCNEEVKFNVFKAMKYPDEMEDCSFIRILES 506
            +VPIILGRPFLATGR LI+VQKGELTMRV ++++ FNVFKAMK+ +E ++C  + + ++
Sbjct: 309 IEVPIILGRPFLATGRTLINVQKGELTMRVQDQQITFNVFKAMKFSNESDECFDVSLFDN 368

Query: 507 TV------------IETAIQDSADKHSEKHGE---------------------------- 566
                         +E A+ D  D+ +E+  E                            
Sbjct: 369 LAGDESIAEQPLDPLERALLDLMDEENEEDLEVVKTLDASKYFKSRGVESLERTAPSKVL 428

Query: 567 ---------------------------------------------------QYRKAIGWT 626
                                                               ++ AIGWT
Sbjct: 429 KPSIEEPPTLELKPLPSHLCYAYLGESDTLPVIISSSFSDLQVENFLRVLRNHKCAIGWT 488

Query: 627 LADIQGISLSFCMHKITLEEGSFRSIEQQRRLNPAMKEVVKKEVIKWLDVGIIYPIADSN 674
           +ADI+GIS SFCMHKI LE+    S++ QRRLNP MKEVVKKE+IKWLDVGIIYPI+DS+
Sbjct: 489 IADIKGISPSFCMHKILLEDDQKPSVKSQRRLNPIMKEVVKKEIIKWLDVGIIYPISDSS 548

BLAST of Lag0015918 vs. ExPASy TrEMBL
Match: A0A2G9HH15 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 2.5e-146
Identity = 321/688 (46.66%), Postives = 382/688 (55.52%), Query Frame = 0

Query: 147  VNQV--TDEACVYCGEDHNYEFCPSNPASVL----AQPPQ-------------------- 206
            VNQV  T   C  CGE H  + CP +  S+     A+ PQ                    
Sbjct: 353  VNQVQHTPVTCEECGEGHPSDQCPHSVESIQFVSNARKPQNNPYSNTYNPGWRQHPNFSW 412

Query: 207  --------LLMGSQGNNVQAQQKMNQ--PGFAK--------------------AQLANEL 266
                     L   QG   Q QQ M +  P   +                     QLAN +
Sbjct: 413  NNNQGQGLALRFQQGGQQQVQQPMQEKKPSLEETLIQFMASTAANFKMMETQIGQLANAI 472

Query: 267  KARPQGKLPSDTE-HPRREGKEQVKAVTLRSGKPLEESRKTQDLNSNRNNNVVIEKELES 326
             +RP+  LPS+TE +PR++ K Q +AVTLR+G  L+E  K    +         EKE+ S
Sbjct: 473  NSRPRRSLPSNTEPNPRQDSKAQCQAVTLRNGTELQEVVKEPTKSK--------EKEVIS 532

Query: 327  GQGAGGSKENAGASGSVPDVEAPYVPPPPYVPPLPFPQRQKPKNRMCDY----------- 386
                    E  G     P         P YV  +        K R+ DY           
Sbjct: 533  --------EEKGKEIEAPLEVKALEQMPSYVKFM--KDILSKKRRLGDYETVALTEECSA 592

Query: 387  --QEWSTPKAKDPGSFTIPVSIGGKELGRALCDLGASINLMPLSVYRKLGIGEASPTIVT 446
              Q    PK KDPGSFTIP +IG    GRALCDLGASINLMP S+YR LG+GEA PT +T
Sbjct: 593  IIQNKLPPKLKDPGSFTIPCTIGTHFSGRALCDLGASINLMPYSIYRTLGLGEAKPTSIT 652

Query: 447  LQLADRSITYPEGKIEDVLVKVDKFIFPVDFIILDYEADKDVPIILGRPFLATGRALIDV 506
            LQLADRS+TYP G IED+LVKVDKFIFP DF++LD E D +VPIILGRPFLATGR LIDV
Sbjct: 653  LQLADRSLTYPNGVIEDILVKVDKFIFPADFVVLDMEVDIEVPIILGRPFLATGRTLIDV 712

Query: 507  QKGELTMRVCNEEVKFNVFKAMKYPDEMEDCSFIRILESTV------------IETAIQD 566
            QKGELTMRV ++++ FNVFKAMK+P+E ++C  + + +               +E A+ D
Sbjct: 713  QKGELTMRVQDQQITFNVFKAMKFPNESDECFAVSLFDKLAGNESIAEQPLDPLERALLD 772

Query: 567  SADKHSEKHGE------------------------------------------------- 626
              D+ +E+  E                                                 
Sbjct: 773  LLDEENEEDCEVVKTLDASKYFKSRGVESLERTAPSKVLKPSIEEPPTLELKPLPSHLCY 832

Query: 627  ------------------------------QYRKAIGWTLADIQGISLSFCMHKITLEEG 674
                                           ++ AIGWT+ADI+GIS SFCMHKI LE+G
Sbjct: 833  AYLGESDTLPVIISSSLSDLQVEKLLRVLRNHKGAIGWTIADIKGISPSFCMHKILLEDG 892

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PIN12235.12.9e-14949.61DNA-directed DNA polymerase [Handroanthus impetiginosus][more]
XP_023522102.13.8e-14945.95LOW QUALITY PROTEIN: uncharacterized protein LOC111785979 [Cucurbita pepo subsp.... [more]
PIN22487.12.5e-14845.55DNA-directed DNA polymerase [Handroanthus impetiginosus][more]
PIN22518.11.6e-14749.59DNA-directed DNA polymerase [Handroanthus impetiginosus][more]
PIN00194.11.0e-14649.00DNA-directed DNA polymerase [Handroanthus impetiginosus][more]
Match NameE-valueIdentityDescription
Q993151.2e-2034.42Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Q7LHG51.2e-2034.42Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
P043232.3e-1624.93Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
P103949.7e-1535.61Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste... [more]
P318434.8e-1450.00RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
A0A2G9H4001.4e-14949.61Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_15163 PE=... [more]
A0A2G9HYA01.2e-14845.55Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04802 PE=... [more]
A0A2G9HYD87.7e-14849.59Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04775 PE=... [more]
A0A2G9G4F15.0e-14749.00DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_273... [more]
A0A2G9HH152.5e-14646.66Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 312..455
e-value: 2.5E-30
score: 107.0
NoneNo IPR availableGENE3D3.10.10.10HIV Type 1 Reverse Transcriptase, subunit A, domain 1coord: 507..674
e-value: 1.3E-39
score: 137.1
NoneNo IPR availablePFAMPF13650Asp_protease_2coord: 334..426
e-value: 1.2E-5
score: 25.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 290..308
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..252
NoneNo IPR availablePANTHERPTHR33067FAMILY NOT NAMEDcoord: 323..471
NoneNo IPR availablePANTHERPTHR33067:SF28SUBFAMILY NOT NAMEDcoord: 323..471
NoneNo IPR availableCDDcd01647RT_LTRcoord: 555..673
e-value: 2.42225E-42
score: 149.283
NoneNo IPR availableCDDcd00303retropepsin_likecoord: 334..428
e-value: 1.46604E-18
score: 78.9175
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 515..674

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0015918.1Lag0015918.1mRNA