Lag0015732 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0015732 vs. NCBI nr
Match: GEU86091.1 (retrovirus-related Pol polyprotein from transposon TNT 1-94 [Tanacetum cinerariifolium])

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-34
Identity = 91/195 (46.67%), Postives = 113/195 (57.95%), Query Frame = 0

Query: 1   MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
           M N F+MS MG+LSFFLGL++KQ KD IFISQ+KY  ++LK F F   K   TP+ T   
Sbjct: 675 MKNRFQMSSMGKLSFFLGLRVKQKKDGIFISQDKYVTEILKTFDFLSVKTVSTPIKTQKP 734

Query: 61  LDKDEE-----------------------------GLWYPRNNAEFKLVGYSDADFAGSL 120
           L KDEE                             GLWYP+ ++ F L  YSD+D+AG+ 
Sbjct: 735 LVKDEEAADVDVTPKTSHLHDVKRIFRYLKGQLKLGLWYPKVSS-FNLEAYSDSDYAGAN 794

Query: 121 LDRL----PYRSNIVN--IYCDNQSTIFLTKNPQFHIRTKHIDINFHFVRDEIEKGEIQV 161
           L+R      Y  N +N  IY DN+STI + KNP FH +TKHI+I  HF+RD  EK  IQV
Sbjct: 795 LERKSTIGDYGFNFMNTKIYIDNESTICVVKNPVFHSKTKHIEIRHHFIRDAYEKKLIQV 854

BLAST of Lag0015732 vs. NCBI nr
Match: CAN70326.1 (hypothetical protein VITISV_041472 [Vitis vinifera])

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-34
Identity = 95/220 (43.18%), Postives = 118/220 (53.64%), Query Frame = 0

Query: 1   MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
           MH EFEMSMMGEL FFLGLQIKQLK+  FI+Q KY  DLLKRF   E K  KTPMS+S K
Sbjct: 164 MHREFEMSMMGELKFFLGLQIKQLKEGTFINQAKYITDLLKRFNMEEAKTMKTPMSSSIK 223

Query: 61  LDKDEEGLWYPRNNAEFKLVGYSDADFAGSLLDR-------------------------- 120
           LD DE+GLWYP+ +  F+L+GYSDADFA   ++R                          
Sbjct: 224 LDMDEKGLWYPKGD-NFELIGYSDADFASCKVERKSTSGICHFLGHSLVSWHSKKQNSVA 283

Query: 121 ------------------LPYRSNI---------VNIYCDNQSTIFLTKNPQFHIRTKHI 168
                             L  +  I         V I CDN S I ++KNP  H RTKHI
Sbjct: 284 LFMAEAEYIAADLCCAQILWMKQKISDFNMIFEHVPIKCDNTSAINISKNPVQHSRTKHI 343

BLAST of Lag0015732 vs. NCBI nr
Match: PWA63219.1 (retrotransposon protein [Artemisia annua])

HSP 1 Score: 156.8 bits (395), Expect = 3.1e-34
Identity = 87/222 (39.19%), Postives = 115/222 (51.80%), Query Frame = 0

Query: 1    MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
            MH+EFEMSMMGEL+FFLGLQIKQL+D IF +Q KY +++LK+F   + K  K PMST  K
Sbjct: 843  MHDEFEMSMMGELNFFLGLQIKQLEDGIFFNQYKYIKEMLKKFGLEDSKPLKKPMSTEIK 902

Query: 61   LDKDEEGLWYPRNNAEFKLVGYSDADFAGSLLDR-------------------------- 120
            L +DEEGLWYP+ +   + + Y+D+D AG  +DR                          
Sbjct: 903  LTRDEEGLWYPKGSG-IETIVYADSDHAGDYVDRKSTSGICTFMGCCLTSWFSKKQTAIA 962

Query: 121  ---------------------------LPYRSNIVNIYCDNQSTIFLTKNPQFHIRTKHI 170
                                          R   + I CDN+  I L+KNP  H RTKHI
Sbjct: 963  ISTTEAEYVSAGKACQQALWMKQALVDYDIRLENIPIMCDNKGAIDLSKNPVQHSRTKHI 1022

BLAST of Lag0015732 vs. NCBI nr
Match: GEU93550.1 (hypothetical protein [Tanacetum cinerariifolium])

HSP 1 Score: 153.7 bits (387), Expect = 2.6e-33
Identity = 95/227 (41.85%), Postives = 123/227 (54.19%), Query Frame = 0

Query: 1    MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
            M N F+MS MGEL+FFLGLQ+KQ +D IFISQ+KY  ++LK+F F   K A TP+ T   
Sbjct: 781  MKNRFQMSSMGELTFFLGLQVKQKEDGIFISQDKYVAEILKKFNFLSVKTASTPIGTQKP 840

Query: 61   LDKDEE------------------GLWYPRNNAEFKLVGYSDADFAGSLLDR-------- 120
            L KDEE                  G+WYP+ ++ F L  Y D+D+A + LDR        
Sbjct: 841  LVKDEEAADVDVHLYRYLKSKPKLGVWYPKVSS-FDLEAYLDSDYACANLDRKAKTGEVE 900

Query: 121  ------------------LPYRSNIVN--IYCDNQSTIFLTKNPQFHIRTKHIDINFHFV 180
                              L Y  N++N  IY DN STI + KN  FH +TKHI+I  HF+
Sbjct: 901  YMAIAHCCGQVLWIQNQLLDYGFNLMNTKIYIDNSSTICIVKNIVFHSKTKHIEIRHHFI 960

Query: 181  RDEIEKGEIQVLKIHTSDNATDMLNKPLTQLKFQHCLQFVGFHLPEK 182
            RD  EK  IQVLKIHT DN  D+L K     + +  L  VGF+  ++
Sbjct: 961  RDACEKKLIQVLKIHTDDNVADLLTKAFDVSRNEGSLS-VGFNTTQQ 1005

BLAST of Lag0015732 vs. NCBI nr
Match: RVW40379.1 (Copia protein [Vitis vinifera])

HSP 1 Score: 153.7 bits (387), Expect = 2.6e-33
Identity = 88/194 (45.36%), Postives = 108/194 (55.67%), Query Frame = 0

Query: 1   MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
           MH+EFEMSMMGEL+FFLGLQIKQLK+  FI+Q KY RDLLKRF   E K  KTPMS+S K
Sbjct: 1   MHSEFEMSMMGELNFFLGLQIKQLKEGTFINQAKYIRDLLKRFNMKEAKTMKTPMSSSIK 60

Query: 61  LDKDEEGLWYPRNNAEFKLVGYSDADFAGSLLDR------------LPYRSNIVN----- 120
           LD DE+GLWYP+ +  F+L+GYSDADF G  ++R            + + S   N     
Sbjct: 61  LDIDEKGLWYPKGD-NFELIGYSDADFTGCKVERKSTSDTCHFLALVSWHSKKQNSVALS 120

Query: 121 ----------------------------------IYCDNQSTIFLTKNPQFHIRTKHIDI 144
                                               CDN S I ++KNP  H RTKHI+I
Sbjct: 121 TTKAEYIAAGLCCAQIHWMKQTLSDFNLIFEHVPTKCDNTSAINISKNPLQHFRTKHIEI 180

BLAST of Lag0015732 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.7e-15
Identity = 34/74 (45.95%), Postives = 53/74 (71.62%), Query Frame = 0

Query: 104  IYCDNQSTIFLTKNPQFHIRTKHIDINFHFVRDEIEKGEIQVLKIHTSDNATDMLNKPLT 163
            +YCD+QS I L+KN  +H RTKHID+ +H++R+ ++   ++VLKI T++N  DML K + 
Sbjct: 1253 VYCDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVLKISTNENPADMLTKVVP 1312

Query: 164  QLKFQHCLQFVGFH 178
            + KF+ C + VG H
Sbjct: 1313 RNKFELCKELVGMH 1326

BLAST of Lag0015732 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.1e-13
Identity = 45/125 (36.00%), Postives = 69/125 (55.20%), Query Frame = 0

Query: 54   PMSTSTKLDKDEEGLWYPRNNAEFKLVG--YSDADFAGSLLDRLPYR-SNIVNIYCDNQS 113
            P+S S+K  K   G+      AE++ V    S+  +  SLL  L  R +    IYCDN  
Sbjct: 1338 PISWSSKKQK---GVVRSSTEAEYRSVANTSSEMQWICSLLTELGIRLTRPPVIYCDNVG 1397

Query: 114  TIFLTKNPQFHIRTKHIDINFHFVRDEIEKGEIQVLKIHTSDNATDMLNKPLTQLKFQHC 173
              +L  NP FH R KHI I++HF+R++++ G ++V+ + T D   D L KPL++  FQ+ 
Sbjct: 1398 ATYLCANPVFHSRMKHIAIDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLSRTAFQNF 1457

Query: 174  LQFVG 176
               +G
Sbjct: 1458 ASKIG 1459

BLAST of Lag0015732 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.1e-13
Identity = 44/125 (35.20%), Postives = 72/125 (57.60%), Query Frame = 0

Query: 54   PMSTSTKLDKDEEGLWYPRNNAEFKLVG--YSDADFAGSLLDRLPYR-SNIVNIYCDNQS 113
            P+S S+K  K   G+      AE++ V    S+  +  SLL  L  + S+   IYCDN  
Sbjct: 1321 PISWSSKKQK---GVVRSSTEAEYRSVANTSSELQWICSLLTELGIQLSHPPVIYCDNVG 1380

Query: 114  TIFLTKNPQFHIRTKHIDINFHFVRDEIEKGEIQVLKIHTSDNATDMLNKPLTQLKFQHC 173
              +L  NP FH R KHI +++HF+R++++ G ++V+ + T D   D L KPL+++ FQ+ 
Sbjct: 1381 ATYLCANPVFHSRMKHIALDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLSRVAFQNF 1440

Query: 174  LQFVG 176
             + +G
Sbjct: 1441 SRKIG 1442

BLAST of Lag0015732 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 59.3 bits (142), Expect = 8.8e-08
Identity = 27/68 (39.71%), Postives = 38/68 (55.88%), Query Frame = 0

Query: 100  NIVNIYCDNQSTIFLTKNPQFHIRTKHIDINFHFVRDEIEKGEIQVLKIHTSDNATDMLN 159
            N + IY DNQ  I +  NP  H R KHIDI +HF R++++   I +  I T +   D+  
Sbjct: 1324 NPIKIYEDNQGCISIANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFT 1383

Query: 160  KPLTQLKF 168
            KPL   +F
Sbjct: 1384 KPLPAARF 1391

BLAST of Lag0015732 vs. ExPASy TrEMBL
Match: A5BMB5 (Reverse transcriptase Ty1/copia-type domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_041472 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 6.7e-35
Identity = 95/220 (43.18%), Postives = 118/220 (53.64%), Query Frame = 0

Query: 1   MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
           MH EFEMSMMGEL FFLGLQIKQLK+  FI+Q KY  DLLKRF   E K  KTPMS+S K
Sbjct: 164 MHREFEMSMMGELKFFLGLQIKQLKEGTFINQAKYITDLLKRFNMEEAKTMKTPMSSSIK 223

Query: 61  LDKDEEGLWYPRNNAEFKLVGYSDADFAGSLLDR-------------------------- 120
           LD DE+GLWYP+ +  F+L+GYSDADFA   ++R                          
Sbjct: 224 LDMDEKGLWYPKGD-NFELIGYSDADFASCKVERKSTSGICHFLGHSLVSWHSKKQNSVA 283

Query: 121 ------------------LPYRSNI---------VNIYCDNQSTIFLTKNPQFHIRTKHI 168
                             L  +  I         V I CDN S I ++KNP  H RTKHI
Sbjct: 284 LFMAEAEYIAADLCCAQILWMKQKISDFNMIFEHVPIKCDNTSAINISKNPVQHSRTKHI 343

BLAST of Lag0015732 vs. ExPASy TrEMBL
Match: A0A6L2NIK7 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Tanacetum cinerariifolium OX=118510 GN=Tci_058069 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 6.7e-35
Identity = 91/195 (46.67%), Postives = 113/195 (57.95%), Query Frame = 0

Query: 1   MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
           M N F+MS MG+LSFFLGL++KQ KD IFISQ+KY  ++LK F F   K   TP+ T   
Sbjct: 675 MKNRFQMSSMGKLSFFLGLRVKQKKDGIFISQDKYVTEILKTFDFLSVKTVSTPIKTQKP 734

Query: 61  LDKDEE-----------------------------GLWYPRNNAEFKLVGYSDADFAGSL 120
           L KDEE                             GLWYP+ ++ F L  YSD+D+AG+ 
Sbjct: 735 LVKDEEAADVDVTPKTSHLHDVKRIFRYLKGQLKLGLWYPKVSS-FNLEAYSDSDYAGAN 794

Query: 121 LDRL----PYRSNIVN--IYCDNQSTIFLTKNPQFHIRTKHIDINFHFVRDEIEKGEIQV 161
           L+R      Y  N +N  IY DN+STI + KNP FH +TKHI+I  HF+RD  EK  IQV
Sbjct: 795 LERKSTIGDYGFNFMNTKIYIDNESTICVVKNPVFHSKTKHIEIRHHFIRDAYEKKLIQV 854

BLAST of Lag0015732 vs. ExPASy TrEMBL
Match: A0A2U1MPN7 (Retrotransposon protein OS=Artemisia annua OX=35608 GN=CTI12_AA355870 PE=4 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 1.5e-34
Identity = 87/222 (39.19%), Postives = 115/222 (51.80%), Query Frame = 0

Query: 1    MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
            MH+EFEMSMMGEL+FFLGLQIKQL+D IF +Q KY +++LK+F   + K  K PMST  K
Sbjct: 843  MHDEFEMSMMGELNFFLGLQIKQLEDGIFFNQYKYIKEMLKKFGLEDSKPLKKPMSTEIK 902

Query: 61   LDKDEEGLWYPRNNAEFKLVGYSDADFAGSLLDR-------------------------- 120
            L +DEEGLWYP+ +   + + Y+D+D AG  +DR                          
Sbjct: 903  LTRDEEGLWYPKGSG-IETIVYADSDHAGDYVDRKSTSGICTFMGCCLTSWFSKKQTAIA 962

Query: 121  ---------------------------LPYRSNIVNIYCDNQSTIFLTKNPQFHIRTKHI 170
                                          R   + I CDN+  I L+KNP  H RTKHI
Sbjct: 963  ISTTEAEYVSAGKACQQALWMKQALVDYDIRLENIPIMCDNKGAIDLSKNPVQHSRTKHI 1022

BLAST of Lag0015732 vs. ExPASy TrEMBL
Match: A0A438DY28 (Copia protein OS=Vitis vinifera OX=29760 GN=GIP_425 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 1.3e-33
Identity = 88/194 (45.36%), Postives = 108/194 (55.67%), Query Frame = 0

Query: 1   MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
           MH+EFEMSMMGEL+FFLGLQIKQLK+  FI+Q KY RDLLKRF   E K  KTPMS+S K
Sbjct: 1   MHSEFEMSMMGELNFFLGLQIKQLKEGTFINQAKYIRDLLKRFNMKEAKTMKTPMSSSIK 60

Query: 61  LDKDEEGLWYPRNNAEFKLVGYSDADFAGSLLDR------------LPYRSNIVN----- 120
           LD DE+GLWYP+ +  F+L+GYSDADF G  ++R            + + S   N     
Sbjct: 61  LDIDEKGLWYPKGD-NFELIGYSDADFTGCKVERKSTSDTCHFLALVSWHSKKQNSVALS 120

Query: 121 ----------------------------------IYCDNQSTIFLTKNPQFHIRTKHIDI 144
                                               CDN S I ++KNP  H RTKHI+I
Sbjct: 121 TTKAEYIAAGLCCAQIHWMKQTLSDFNLIFEHVPTKCDNTSAINISKNPLQHFRTKHIEI 180

BLAST of Lag0015732 vs. ExPASy TrEMBL
Match: A0A6L2P531 (Integrase catalytic domain-containing protein (Fragment) OS=Tanacetum cinerariifolium OX=118510 GN=Tci_065528 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 1.3e-33
Identity = 95/227 (41.85%), Postives = 123/227 (54.19%), Query Frame = 0

Query: 1    MHNEFEMSMMGELSFFLGLQIKQLKDDIFISQEKYTRDLLKRFKFNEGKIAKTPMSTSTK 60
            M N F+MS MGEL+FFLGLQ+KQ +D IFISQ+KY  ++LK+F F   K A TP+ T   
Sbjct: 781  MKNRFQMSSMGELTFFLGLQVKQKEDGIFISQDKYVAEILKKFNFLSVKTASTPIGTQKP 840

Query: 61   LDKDEE------------------GLWYPRNNAEFKLVGYSDADFAGSLLDR-------- 120
            L KDEE                  G+WYP+ ++ F L  Y D+D+A + LDR        
Sbjct: 841  LVKDEEAADVDVHLYRYLKSKPKLGVWYPKVSS-FDLEAYLDSDYACANLDRKAKTGEVE 900

Query: 121  ------------------LPYRSNIVN--IYCDNQSTIFLTKNPQFHIRTKHIDINFHFV 180
                              L Y  N++N  IY DN STI + KN  FH +TKHI+I  HF+
Sbjct: 901  YMAIAHCCGQVLWIQNQLLDYGFNLMNTKIYIDNSSTICIVKNIVFHSKTKHIEIRHHFI 960

Query: 181  RDEIEKGEIQVLKIHTSDNATDMLNKPLTQLKFQHCLQFVGFHLPEK 182
            RD  EK  IQVLKIHT DN  D+L K     + +  L  VGF+  ++
Sbjct: 961  RDACEKKLIQVLKIHTDDNVADLLTKAFDVSRNEGSLS-VGFNTTQQ 1005