Lag0015678 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015678
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Location	chr12: 19886841 .. 19887173 (+)
RNA-Seq Expression	Lag0015678
Synteny	Lag0015678

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTGTCCTGAAAGCTCGAAAAGCCCTGGTCTTCTCGATCATCGCCCATGTCTCATTGCTAGCCTGTGCTTCTTCTCACGAGCTTCAAATCATCAACGCAGAGAAAATAATTTACTTGACTTCTCACATTGTGAAGGTTTTCCTTACATTGAAGGTGGAAAATATTGGAATGTTTCCAGCTTCAAATGTCCTTCTTGCTTTTCCACCTACAAAATTTAATCATCTATCACTAGTTAAGGTTGTTGTTGCTTCTGGAAAGAAGAAGAAAAAGTCTTATATTCCCTTTGATGTAGGAACGATAGAGAAAAAGAATGTTGGGAGAAGATAG

mRNA sequence

ATGGAAGCTGTCCTGAAAGCTCGAAAAGCCCTGGTCTTCTCGATCATCGCCCATGTCTCATTGCTAGCCTGTGCTTCTTCTCACGAGCTTCAAATCATCAACGCAGAGAAAATAATTTACTTGACTTCTCACATTGTGAAGGTTTTCCTTACATTGAAGGTGGAAAATATTGGAATGTTTCCAGCTTCAAATGTCCTTCTTGCTTTTCCACCTACAAAATTTAATCATCTATCACTAGTTAAGGTTGTTGTTGCTTCTGGAAAGAAGAAGAAAAAGTCTTATATTCCCTTTGATGTAGGAACGATAGAGAAAAAGAATGTTGGGAGAAGATAG

Coding sequence (CDS)

ATGGAAGCTGTCCTGAAAGCTCGAAAAGCCCTGGTCTTCTCGATCATCGCCCATGTCTCATTGCTAGCCTGTGCTTCTTCTCACGAGCTTCAAATCATCAACGCAGAGAAAATAATTTACTTGACTTCTCACATTGTGAAGGTTTTCCTTACATTGAAGGTGGAAAATATTGGAATGTTTCCAGCTTCAAATGTCCTTCTTGCTTTTCCACCTACAAAATTTAATCATCTATCACTAGTTAAGGTTGTTGTTGCTTCTGGAAAGAAGAAGAAAAAGTCTTATATTCCCTTTGATGTAGGAACGATAGAGAAAAAGAATGTTGGGAGAAGATAG

Protein sequence

MEAVLKARKALVFSIIAHVSLLACASSHELQIINAEKIIYLTSHIVKVFLTLKVENIGMFPASNVLLAFPPTKFNHLSLVKVVVASGKKKKKSYIPFDVGTIEKKNVGRR

Homology

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BLAST of Lag0015678 vs. NCBI nr
Match: XP_022157433.1 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Momordica charantia])

HSP 1 Score: 144.1 bits (362), Expect = 7.4e-31
Identity = 75/103 (72.82%), Postives = 90/103 (87.38%), Query Frame = 0


Query: 1   MEAVLKARKALVFSIIAHVSLLACASSHELQIINAEKIIYLTSHIVKVFLTLKVENIGMF 60
           ME +LKAR ALVFSI+AHVSLL  ASSH+LQI+NAEK I LTSHIV+VFLTLKVEN+G  
Sbjct: 17  METLLKARLALVFSILAHVSLLTSASSHQLQILNAEKRIDLTSHIVRVFLTLKVENVGTL 76

Query: 61  PASNVLLAFPPTKFNHLSLVKVVVASGKKKKKSYIPFDVGTIE 104
           PAS+VLLAFPPT+F+HL++VK  +A+GKKKK+SY+P DV  IE
Sbjct: 77  PASDVLLAFPPTEFDHLAVVKAAIATGKKKKRSYVPLDVKPIE 119

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BLAST of Lag0015678 vs. NCBI nr
Match: XP_038893323.1 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Benincasa hispida])

HSP 1 Score: 142.1 bits (357), Expect = 2.8e-30
Identity = 77/103 (74.76%), Postives = 87/103 (84.47%), Query Frame = 0


Query: 1   MEAVLKARKALVFSIIAHVSLLACASSHELQIINAEKIIYLTSHIVKVFLTLKVENIGMF 60
           ME   KAR ALVFSIIAH+SLLACASSHELQI+NAEK I LTSHIVKVFLTLKVEN G  
Sbjct: 1   METQPKARLALVFSIIAHLSLLACASSHELQIVNAEKRIDLTSHIVKVFLTLKVENTGTV 60

Query: 61  PASNVLLAFPPTKFNHLSLVKVVVASGKKKKKSYIPFDVGTIE 104
           PAS+VLLAFPPT+F+ LSLV+  +A+GKKKK+SY+P DV   E
Sbjct: 61  PASDVLLAFPPTEFDRLSLVRAAIATGKKKKRSYVPLDVKPTE 103

[Close]

BLAST of Lag0015678 vs. NCBI nr
Match: XP_004142408.1 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Cucumis sativus] >KGN52267.1 hypothetical protein Csa_008242 [Cucumis sativus])

HSP 1 Score: 133.7 bits (335), Expect = 1.0e-27
Identity = 73/103 (70.87%), Postives = 84/103 (81.55%), Query Frame = 0


Query: 1   MEAVLKARKALVFSIIAHVSLLACASSHELQIINAEKIIYLTSHIVKVFLTLKVENIGMF 60
           M+ +LK R ALVFSI+AHV  LA ASSH+LQI+NAEK I LTSHIVKVFLTLKVEN G  
Sbjct: 17  MDTLLKPRLALVFSILAHVFFLAFASSHDLQILNAEKRIDLTSHIVKVFLTLKVENTGTL 76

Query: 61  PASNVLLAFPPTKFNHLSLVKVVVASGKKKKKSYIPFDVGTIE 104
           PAS VLLAF PT+F+ LSLVK  +A+GKKKK+SY+P DV  IE
Sbjct: 77  PASEVLLAFSPTEFDRLSLVKAAIATGKKKKRSYVPLDVKPIE 119

[Close]

BLAST of Lag0015678 vs. NCBI nr
Match: XP_022966787.1 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Cucurbita maxima])

HSP 1 Score: 131.0 bits (328), Expect = 6.5e-27
Identity = 73/104 (70.19%), Postives = 84/104 (80.77%), Query Frame = 0


Query: 1   MEAVLKARKALVFSIIAHVSLLACASSHELQIINAEKIIYLTSHIVKVFLTLKVENIGMF 60
           ME +LKAR AL+FSIIAHVSLLA AS  ELQI+NAEK I L S IVKVFLTLKVEN G  
Sbjct: 1   MEFLLKARLALIFSIIAHVSLLAFASPPELQIVNAEKRIDLNSQIVKVFLTLKVENTGTS 60

Query: 61  PASNVLLAFPPTKFNHLSLVKVVVASGKKKKKSYIPFDVGTIEK 105
           PAS++LLAFPPT+ +HLSL+K  +A+ KKKKKSY+P DV   EK
Sbjct: 61  PASDILLAFPPTEIDHLSLIKAAIATRKKKKKSYLPLDVKQTEK 104

[Close]

BLAST of Lag0015678 vs. NCBI nr
Match: XP_023542337.1 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 129.8 bits (325), Expect = 1.4e-26
Identity = 72/104 (69.23%), Postives = 84/104 (80.77%), Query Frame = 0


Query: 1   MEAVLKARKALVFSIIAHVSLLACASSHELQIINAEKIIYLTSHIVKVFLTLKVENIGMF 60
           ME +LKAR AL+FSIIAHVSLLA AS  ELQI+NAEK I L S IVKVFLTLKVEN G  
Sbjct: 1   MEILLKARLALIFSIIAHVSLLAFASPPELQIVNAEKRIDLNSQIVKVFLTLKVENTGTS 60

Query: 61  PASNVLLAFPPTKFNHLSLVKVVVASGKKKKKSYIPFDVGTIEK 105
           PAS++LLAFPPT+ +HLSL+K  +A+ KKKKKSY+P DV   E+
Sbjct: 61  PASDILLAFPPTEIDHLSLIKAAIATRKKKKKSYLPLDVKPTEQ 104

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BLAST of Lag0015678 vs. ExPASy Swiss-Prot
Match: Q9ZUA0 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B OS=Arabidopsis thaliana OX=3702 GN=OST1B PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 5.2e-19
Identity = 51/95 (53.68%), Postives = 72/95 (75.79%), Query Frame = 0


Query: 12  VFSIIAHV--SLLACASSHELQIINAEKIIYLTSHIVKVFLTLKVENIGMFPASNVLLAF 71
           +FS+   V  S+ A +S+ +LQI+NAE+ I L+SHIVK FLTLKVENIG  PA+ +LLAF
Sbjct: 7   IFSVFVAVLLSISAFSSAQDLQIVNAERRIDLSSHIVKAFLTLKVENIGKDPAAEMLLAF 66

Query: 72  PPTKFNHLSLVKVVVASGKKKKKSYIPFDVGTIEK 105
           PPT+  +L++V+ +  +GKKKKK+Y+P DV   E+
Sbjct: 67  PPTQIKNLAMVQALATTGKKKKKTYLPLDVKPTEQ 101

[Close]

BLAST of Lag0015678 vs. ExPASy Swiss-Prot
Match: B9FDT1 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B OS=Oryza sativa subsp. japonica OX=39947 GN=OST1B PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.6e-12
Identity = 42/71 (59.15%), Postives = 52/71 (73.24%), Query Frame = 0


Query: 30  LQIINAEKIIYLTSHIVKVFLTLKVENIGMFP-ASNVLLAFPPTKFNHLSLVKVVVASGK 89
           +++I+AEK I LTS IVKVFLTLK+EN    P AS VLLAF PT+  HL++VK   A GK
Sbjct: 35  IRVISAEKRIDLTSPIVKVFLTLKLENDATAPEASQVLLAFTPTEVEHLAIVKATRAEGK 94

Query: 90  KKKKSYIPFDV 100
           +KKK Y+P  V
Sbjct: 95  RKKKIYVPLSV 105

The following BLAST results are available for this feature:

BLAST of Lag0015678 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157433.1	7.4e-31	72.82	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Momor...	[more]
XP_038893323.1	2.8e-30	74.76	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Benin...	[more]
XP_004142408.1	1.0e-27	70.87	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Cucum...	[more]
XP_022966787.1	6.5e-27	70.19	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Cucur...	[more]
XP_023542337.1	1.4e-26	69.23	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [Cucur...	[more]

BLAST of Lag0015678 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZUA0	5.2e-19	53.68	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B OS=Ara...	[more]
B9FDT1	1.6e-12	59.15	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B OS=Ory...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007676	Ribophorin I	PFAM	PF04597	Ribophorin_I	coord: 31..94 e-value: 3.7E-8 score: 33.0
IPR007676	Ribophorin I	PANTHER	PTHR21049	RIBOPHORIN I	coord: 7..105
None	No IPR available	PANTHER	PTHR21049:SF2	DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1B	coord: 7..105
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..24 score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015678.1	Lag0015678.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018279	protein N-linked glycosylation via asparagine
biological_process	GO:0006486	protein glycosylation
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0008250	oligosaccharyltransferase complex
molecular_function	GO:0016740	transferase activity