Lag0015667 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015667
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cytochrome b5
Location	chr12: 19369129 .. 19371029 (-)
RNA-Seq Expression	Lag0015667
Synteny	Lag0015667

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCAAATTCAAAAGTGTATACTTTCGATGAGGTTGCAAAGCACAACAGCCAGAAAGATTGCTGGCTTATAATATCTGGATATGTATGTTTTCTTTTCTTTTATTTCTTTTACCTTTACAATCCTGGCTATCTGAAAAGTTTGAAAAAAGTTATGCAATTGGTGTAGATTGAGTTCGATTACGTTCTAAGCTTCATTTGAGTTCATTTTGTCAATGGATTTTTCTAAAATTAGTCCGGGTTCAAAACAAATCAACTAAAGTTATTTTTATTTATGATCATCATTAAATCTATAATTAGTGTATCATTAAATCTATAATTAGTGTTTGATAATGTGTATTTCATCATTAAATCTACAATTAAAAAAGTTAATTGACATTCCAACTATATAATAAAGATTTTGATGAAATATACAAAATTTCTGCTTATTTCATAATTTAAATATTTAGTTTAAATACTATTTTAATCCATATACTTTTAACTTTGATTTTATTAATTCATTTTGGTTCTTGTACTTTAAAGATAAACATTTTGATCTCTGTTTGTAAAATTTTAACTAAATTTTATACACAAACAAAAACATTTTAATAAAAAGTTATAGCTCTTTCAAAAAGAAAATAATAATAAATAAATAAATAAATAAAATCAAAGTTATAGCTATATTTTAAATAACTTTTTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAAAAAAAAAATAACTTTTTGAGTGTAGTGTTGTAATAAAATGTTTATTTAAAAGAGAGAGAACTTTTTTTGTAAAAAAAAGAAAACAAATATAGTCACATAGCATAAGATGAAAATAGACGTTGTATAGAAACTAAAATTATATTTAAACCTATATAATTTTAACCATTTTTTTAAATTTCTTAACAGTTTTCAAATATTCATTTACATCCTTATTTTATTGATATTTAGGTAAATATTTAAAAAAAAACAATTTCTTGTTTTGCTTATTTCTTTTTTGGGCTACAGTCCTAATTCTGTATGTGCATTGGTTTTAGGTGTACGATGTGACTTCTTTTTTGCCGGACCATCCAGGAGGAGATGAGCTTTTACTACTTGCAGTTGGTAAGATATGATACTGCATTTCTAATAATACAAATTATGTAATTAATTACCCTTTTTAACAATATTTTTTTTAAAACCCTTTTTAACAAGCTTAATTAGTAAAATAACATATAAATTAAAATGTGTTTAATACATAGGATTTTTTTCCCTTTATTGTGTTAGGTTTTTTAAATTCTAAAAAGTGTTTATTCTACCTTAATTTAGGGTGTGTTTGGTTTAACTTTCCAAATATTTAATTATGAAAATAAATCATTTTGAAAAAATTATAGTGTTTGACAACCACTCAAAATGACTTATTAAAAAGTGAGATTGATAAATAAACCATTTTGAGAGAAACACTTGAAACCAACTTTTAAAAAAAGACTTCTTAAGTAAACAATAACTTGTGTATAAAAACATTTGTGAGGAAATGCAACAAAACATATGAGTGTTTAAATTCTAAACAATGTTTATACAAAATGTTTTTTAAAAAATGCATTCTTGAAAAACATTTAAATTAAAATGTATTCCAAACGGACCCTTAATTACGTTGAATTTATGACATCTGAATTTGTAGAAAAAGATGCAACATTTGATTTCAAAAGCGTTGGACACAGTGAGTTAGCTCAAGAGAAGATGAAAAAGTTTCGGATTGGAAAGATCGACCCATCGACTCTTCCAAGAAAACAAGAATATGTGGAAACTACCCCACACCAACCAACTCCAAGTCTCTTTTCTTTTTCACCTGTGCATGTTCTAATCCCTATGTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTTAAACTCAAGGCCTAG

mRNA sequence

ATGGATTCAAATTCAAAAGTGTATACTTTCGATGAGGTTGCAAAGCACAACAGCCAGAAAGATTGCTGGCTTATAATATCTGGATATGTGTACGATGTGACTTCTTTTTTGCCGGACCATCCAGGAGGAGATGAGCTTTTACTACTTGCAGTTGAAAAAGATGCAACATTTGATTTCAAAAGCGTTGGACACAGTGAGTTAGCTCAAGAGAAGATGAAAAAGTTTCGGATTGGAAAGATCGACCCATCGACTCTTCCAAGAAAACAAGAATATGTGGAAACTACCCCACACCAACCAACTCCAAGTCTCTTTTCTTTTTCACCTGTGCATGTTCTAATCCCTATGTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTTAAACTCAAGGCCTAG

Coding sequence (CDS)

ATGGATTCAAATTCAAAAGTGTATACTTTCGATGAGGTTGCAAAGCACAACAGCCAGAAAGATTGCTGGCTTATAATATCTGGATATGTGTACGATGTGACTTCTTTTTTGCCGGACCATCCAGGAGGAGATGAGCTTTTACTACTTGCAGTTGAAAAAGATGCAACATTTGATTTCAAAAGCGTTGGACACAGTGAGTTAGCTCAAGAGAAGATGAAAAAGTTTCGGATTGGAAAGATCGACCCATCGACTCTTCCAAGAAAACAAGAATATGTGGAAACTACCCCACACCAACCAACTCCAAGTCTCTTTTCTTTTTCACCTGTGCATGTTCTAATCCCTATGTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTTAAACTCAAGGCCTAG

Protein sequence

MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQPTPSLFSFSPVHVLIPMLLLALAFAFPYFKLKA

Homology

[Close]

BLAST of Lag0015667 vs. NCBI nr
Match: XP_008459741.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 247.3 bits (630), Expect = 7.4e-62
Identity = 119/131 (90.84%), Postives = 127/131 (96.95%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHN QKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQPTPSLFSFSPVHVLIPMLLLAL 120
           SVGHSELAQEKMK +RIGKID STLP+KQ YVE+TP+QPTPSLFSFSP+HVL+P+LLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Lag0015667 vs. NCBI nr
Match: XP_038875836.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 238.8 bits (608), Expect = 2.6e-59
Identity = 113/131 (86.26%), Postives = 127/131 (96.95%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDS+SK+Y+FDEV+KHN  +DCWLIISGYVYDVT+FLPDHPGG+ELLLLAV +DATFDFK
Sbjct: 3   MDSSSKLYSFDEVSKHNHHRDCWLIISGYVYDVTAFLPDHPGGEELLLLAVGRDATFDFK 62

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQPTPSLFSFSPVHVLIPMLLLAL 120
           SVGHSELAQEKMKK+RIGKID STLPRKQ+YVETTP+QPTPSLFSFSP+H+LIP+LLLAL
Sbjct: 63  SVGHSELAQEKMKKYRIGKIDMSTLPRKQQYVETTPYQPTPSLFSFSPIHLLIPILLLAL 122

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 123 AFAFPYFKLKA 133

[Close]

BLAST of Lag0015667 vs. NCBI nr
Match: XP_004140502.1 (cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cucumis sativus])

HSP 1 Score: 237.3 bits (604), Expect = 7.7e-59
Identity = 114/131 (87.02%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHN QKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQPTPSLFSFSPVHVLIPMLLLAL 120
           SVGHSELA EKMK ++IGKID STLP KQ+YVE+ P+Q TPSLFSFSP+H+L+P+LLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Lag0015667 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 229.6 bits (584), Expect = 1.6e-56
Identity = 108/131 (82.44%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYTFDEVAKHN +KDCWLIISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQPTPSLFSFSPVHVLIPMLLLAL 120
           SVGHSE A+E+MKK++IGKIDP TLP +Q++VETTPH+PTPSL SFSP+HVLIP+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Lag0015667 vs. NCBI nr
Match: KAG6593603.1 (Cycloartenol synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 227.6 bits (579), Expect = 6.1e-56
Identity = 107/131 (81.68%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSK++TFDEVAKHN +KDCWLIISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 584 MDSNSKLFTFDEVAKHNHEKDCWLIISGHVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 643

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQPTPSLFSFSPVHVLIPMLLLAL 120
           SVGHSE AQE MKK++IGKIDP TLP +Q++VETTPH+PTPSL SFSP+HVLIP+LLL L
Sbjct: 644 SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 703

Query: 121 AFAFPYFKLKA 132
           AFAFPY+K+KA
Sbjct: 704 AFAFPYWKIKA 714

[Close]

BLAST of Lag0015667 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-28
Identity = 68/136 (50.00%), Postives = 90/136 (66.18%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV +F+EV+KHN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQP------TPSLFSFSPVHVLIP 120
            VGHS+ A++ M K+ IG+ID S++P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 MLLLALAFAFPYFKLK 131
           +L+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of Lag0015667 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 9.5e-28
Identity = 68/136 (50.00%), Postives = 88/136 (64.71%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S  KV  F+EV++HN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQP------TPSLFSFSPVHVLIP 120
            VGHS+ A++ M+K+ IG+ID ST+P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPE-FMIKILQFLVP 120

Query: 121 MLLLALAFAFPYFKLK 131
           +L+L LA     +  K
Sbjct: 121 ILILGLALVVRQYTKK 133

[Close]

BLAST of Lag0015667 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.1e-27
Identity = 62/132 (46.97%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +KV+T  EV++HN+ KDCWL+ISG VYDVT FL DHPGGDE+LL A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQP-----TPSLFSFSPVHVLIPM 120
            VGHS  A+  + ++ +G ID +T+P K +Y  T P+QP       S F    +  L+P+
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY--TPPNQPHYNQDKTSEFVVKLLQFLVPL 120

Query: 121 LLLALAFAFPYF 128
           ++L +AF   ++
Sbjct: 121 IILGVAFGIRFY 130

[Close]

BLAST of Lag0015667 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 2.8e-27
Identity = 59/126 (46.83%), Postives = 86/126 (68.25%), Query Frame = 0


Query: 1   MDSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K++T  EV++HN   DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQP-----TPSLFSFSPVHVLIPM 120
            VGHSE A+E M+++ +G+IDP+T+P+K +Y  T P QP       S F    +  L+P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLLALA 122
            +L LA
Sbjct: 121 AILGLA 124

[Close]

BLAST of Lag0015667 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 8.0e-27
Identity = 64/128 (50.00%), Postives = 84/128 (65.62%), Query Frame = 0


Query: 2   DSNSKVYTFDEVAKHNSQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKS 61
           + N KVYT +EVAKHNS+ DCWLII G VY+V+ FL DHPGGD++LL +  KDAT DF+ 
Sbjct: 3   NDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFED 62

Query: 62  VGHSELAQEKMKKFRIGKIDPSTLPRKQEYVETTPHQP------TPSLFSFSPVHVLIPM 121
           VGHS  A+  M ++ +G ID ST+P + +YV   P QP      TP  F    +  L+P+
Sbjct: 63  VGHSTTARAMMDEYYVGDIDTSTIPARTKYV--PPKQPHYNQDKTPE-FIIKILQFLVPL 122

Query: 122 LLLALAFA 124
            +L LA A
Sbjct: 123 AILGLAVA 127

The following BLAST results are available for this feature:

BLAST of Lag0015667 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459741.1	7.4e-62	90.84	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
XP_038875836.1	2.6e-59	86.26	cytochrome b5-like [Benincasa hispida]	[more]
XP_004140502.1	7.7e-59	87.02	cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cuc...	[more]
XP_022964486.1	1.6e-56	82.44	cytochrome b5-like [Cucurbita moschata]	[more]
KAG6593603.1	6.1e-56	81.68	Cycloartenol synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0015667 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	1.5e-28	50.00	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	9.5e-28	50.00	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	2.1e-27	46.97	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O48845	2.8e-27	46.83	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49100	8.0e-27	50.00	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 68..80 score: 33.1 coord: 30..40 score: 64.74 coord: 55..62 score: 35.8 coord: 40..54 score: 44.55
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 2.2E-27 score: 107.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 10..80 e-value: 2.7E-22 score: 78.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26.946012
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..86 e-value: 2.4E-32 score: 112.8
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..87
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..129
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 1..129
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015667.1	Lag0015667.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding