Lag0015630 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0015630 vs. NCBI nr
Match: RVW96282.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 136.0 bits (341), Expect = 3.5e-28
Identity = 75/199 (37.69%), Postives = 100/199 (50.25%), Query Frame = 0

Query: 9   QESDPQVTSDHWPLIFKAGSVRWCPFPSRLRTCGL------------------NTLPLNL 68
           QE  P+ TSDHWP++      +W P P R     L                  N    N+
Sbjct: 465 QEVLPRWTSDHWPIVLDTNPFKWGPTPFRFENMWLQHHNFKESFSSWWREFEGNGWEANI 524

Query: 69  PSF--LGGIGRCLVSGKASAYGMVSGRGSKNFINSLVNEKGEVVKGSKEIEQEIFLFFSN 128
            +    G +   L + +A   GM +GR +KNFI  L NE+G V+  S+ I +EI L+F  
Sbjct: 525 DAIEQEGALSSDLAAQRAIRKGMANGRRNKNFIKILENERGLVLDSSESITEEILLYFKK 584

Query: 129 LYDPPVVDRPFVNGLDWCPILHRERRELESPFTVEEIKRAILDCDKNKSRGPDGFSMGFF 188
           LY  P  +   V G+DW PI       L+SPF   EI  AI   D++K+ GPDGF++  F
Sbjct: 585 LYSCPTRESWRVEGIDWSPISEESASRLDSPFAEAEIFNAIFQLDRDKAPGPDGFTIAVF 644

BLAST of Lag0015630 vs. NCBI nr
Match: RVW43723.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-26
Identity = 77/225 (34.22%), Postives = 105/225 (46.67%), Query Frame = 0

Query: 9   QESDPQVTSDHWPLIFKAGSVRWCPFPSRLRTCGLNTLPLNLPSFLGGIG---------- 68
           QE+  + TSDHWP++       W P P R     LNTL       +GG+G          
Sbjct: 185 QEALIRRTSDHWPIVMDTNPFMWGPTPFRFENMWLNTLISRRTLEIGGVGFKETDGKEGG 244

Query: 69  --RCLVSGKASAYG----------------------------------MVSGRGSKNFIN 128
               L+S +AS  G                                  + +GR ++ +I 
Sbjct: 245 LNPDLLSQRASRKGELEELILREEIHWRQKAKVKWVKEGDCNSKFYHKVANGRRNRKYIK 304

Query: 129 SLVNEKGEVVKGSKEIEQEIFLFFSNLYDPPVVDRPFVNGLDWCPILHRERRELESPFTV 188
            L NE+G V+K ++ I +EI  +F  LY  P  +   V GLDW PI       L+SPFT 
Sbjct: 305 ELENERGLVLKNAESITEEILHYFEKLYTNPTGESWGVEGLDWSPISEESALRLDSPFTE 364

BLAST of Lag0015630 vs. NCBI nr
Match: CAN71492.1 (hypothetical protein VITISV_005343 [Vitis vinifera])

HSP 1 Score: 129.8 bits (325), Expect = 2.5e-26
Identity = 68/178 (38.20%), Postives = 96/178 (53.93%), Query Frame = 0

Query: 9   QESDPQVTSDHWPLIFKAGSVRWCPFPSRLRTCGLNTLPLNLPSFLGGIGRCLVSGKASA 68
           QE  P+ TSDHW ++ +    +W P P R        + L  P+F    G      + + 
Sbjct: 300 QEVLPRWTSDHWSIVLETNPFKWGPTPFR-----FENMWLQHPNFKERFGSWWRELQGNG 359

Query: 69  YGMVSGRGSKNFINSLVNEKGEVVKGSKEIEQEIFLFFSNLYDPPVVDRPFVNGLDWCPI 128
           + + +GR ++ +I  L NE+G V+  SK I++EI  +F  LY  P  +   V GL+W PI
Sbjct: 360 WEVANGRRNRKYIKVLENERGLVLNNSKSIKEEILRYFEKLYASPSEESWRVKGLNWSPI 419

Query: 129 LHRERRELESPFTVEEIKRAILDCDKNKSRGPDGFSMGFFQANWDTIKVDLERVFKEF 187
                  LESPFT +EI +A    D +K+ GPDGF++  FQ  WD IK DL RVF EF
Sbjct: 420 SGESAPRLESPFTEKEISKATFQLDMDKAPGPDGFTITVFQDCWDVIKEDLVRVFAEF 472

BLAST of Lag0015630 vs. NCBI nr
Match: RVW47466.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 129.4 bits (324), Expect = 3.3e-26
Identity = 71/197 (36.04%), Postives = 98/197 (49.75%), Query Frame = 0

Query: 9   QESDPQVTSDHWPLIFKAGSVRWCPFPSRLRTCGLNTLPLNLPSFLGGIGRCLVSGK--- 68
           QE  P+ TSDHWP++      +W P P RLR CG N +       +GG     + GK   
Sbjct: 28  QEVLPKWTSDHWPIVLDTNPFKWGPTPFRLRICGCNIIVSKSALIVGGENLKEMVGKERK 87

Query: 69  ---ASAYGMVSGRGSKNFINS------------LVNEKGEVVKGSKEIEQEIFLFFSNLY 128
                   ++     +  ++S            + NE+G V+  S+ I +EI L+F  LY
Sbjct: 88  KTILDEIAIIDANEQEGALSSDLAVQRAEFHQIIENERGLVLDNSESITEEILLYFKKLY 147

Query: 129 DPPVVDRPFVNGLDWCPILHRERRELESPFTVEEIKRAILDCDKNKSRGPDGFSMGFFQA 188
             P  +   V G+DW PI       L+SPF+  EI  AI   DK+K+ GPDGF++  FQ 
Sbjct: 148 SSPPDESWRVEGIDWSPISEESVSRLDSPFSEVEIYNAIFQLDKDKAPGPDGFTIAVFQD 207

BLAST of Lag0015630 vs. NCBI nr
Match: RVW95846.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 125.9 bits (315), Expect = 3.6e-25
Identity = 76/235 (32.34%), Postives = 102/235 (43.40%), Query Frame = 0

Query: 9   QESDPQVTSDHWPLIFKAGSVRWCPFPSRLRTCGLNTLPLNLPSFLGGIGRCLVSGKASA 68
           QE+  + TSDHWP+        W P P RLRTCG N L       +GG       GKA+ 
Sbjct: 214 QETLIRRTSDHWPIALDTNPFTWGPTPFRLRTCGCNILVSRRTLEIGGGVFKETDGKATT 273

Query: 69  --------------------------------------------------------YGMV 128
                                                                   + + 
Sbjct: 274 NLDAIEQDGGLTFELLGQRALRKGELEDLILREEIHWRQKARVKWVKEGDCNSKFFHKVA 333

Query: 129 SGRGSKNFINSLVNEKGEVVKGSKEIEQEIFLFFSNLYDPPVVDRPFVNGLDWCPILHRE 188
           +GR ++ +I SL NE G V+  +  I +EI L+F  LY  P+ +   + GLDW PI    
Sbjct: 334 NGRRNRKYIKSLENETGLVLNNAMSITEEILLYFEKLYANPIGESWSIEGLDWSPISEES 393

BLAST of Lag0015630 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.1e-11
Identity = 39/128 (30.47%), Postives = 64/128 (50.00%), Query Frame = 0

Query: 64  GKASAYGMVSGRGSKNFINSLVNEKGEVVKGSKEIEQEIFLFFSNLYDPPVVDRPFVNGL 123
           G    Y +   +G++  I  L  E G  ++  + I      F+ NL+ P  +       L
Sbjct: 371 GSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDRARSFYQNLFSPDPISPDACEEL 430

Query: 124 -DWCPILHRERRE-LESPFTVEEIKRAILDCDKNKSRGPDGFSMGFFQANWDTIKVDLER 183
            D  P++   R+E LE+P T++E+ +A+     NKS G DG ++ FFQ  WDT+  D  R
Sbjct: 431 WDGLPVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHR 490

Query: 184 VFKEFYER 190
           V  E +++
Sbjct: 491 VLTEAFKK 498

BLAST of Lag0015630 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 3.6e-07
Identity = 39/128 (30.47%), Postives = 58/128 (45.31%), Query Frame = 0

Query: 71  MVSGRGSKNFINSLVNEKGEVVKGSKEIEQEIFLFFSNLYDPPV---------VDRPFVN 130
           +  G   K  IN + NEKG++    +EI+  I  F+  LY   +         +DR  V 
Sbjct: 387 LTKGHRDKILINKIRNEKGDITTDPEEIQNTIRSFYKRLYSTKLENLDEMDKFLDRYQVP 446

Query: 131 GLDWCPILHRERRELESPFTVEEIKRAILDCDKNKSRGPDGFSMGFFQANWDTIKVDLER 190
            L+   + H     L SP + +EI+  I      KS GPDGFS  F+Q    T K DL  
Sbjct: 447 KLNQDQVDH-----LNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQ----TFKEDLIP 505

BLAST of Lag0015630 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 5.7e-05
Identity = 32/118 (27.12%), Postives = 59/118 (50.00%), Query Frame = 0

Query: 71  MVSGRGSKNFINSLVNEKGEVVKGSKEIEQEIFLFFSNLYDPPVVDRPFVNG-LDWCPIL 130
           ++  +  KN I+++ N+KG++     EI+  I  ++ +LY   + +   ++  LD   + 
Sbjct: 380 LIKKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLP 439

Query: 131 HRERRELES---PFTVEEIKRAILDCDKNKSRGPDGFSMGFFQANWDTIKVDLERVFK 185
              + E+ES   P T  EI   I      KS GPDGF+  F+Q   + +   L ++F+
Sbjct: 440 RLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQ 497

BLAST of Lag0015630 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.7e-04
Identity = 30/115 (26.09%), Postives = 55/115 (47.83%), Query Frame = 0

Query: 78  KNFINSLVNEKGEVVKGSKEIEQEIFLFFSNLYDPPVVDRPFVNG-LDWCPILHRERREL 137
           K+ I+S+ N   E+     EI++ +  ++  LY     +   ++  L+ C +    ++E+
Sbjct: 386 KSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQKEV 445

Query: 138 E---SPFTVEEIKRAILDCDKNKSRGPDGFSMGFFQANWDTIKVDLERVFKEFYE 189
           E    P +  EI   I +  K KS GPDGF+  F+Q    T K +L  +    ++
Sbjct: 446 EMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQ----TFKEELVPILLNLFQ 496