Lag0015565 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015565
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	major allergen Pru ar 1-like
Location	chr12: 16348574 .. 16349208 (+)
RNA-Seq Expression	Lag0015565
Synteny	Lag0015565

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGTTCAAGGTTTTCCCACTCAAGTCATCTCCATCATTCCCCCAGCCAAGGCTTTCAAGGCCCTCGTCCTCGACTTCAACCAACTCGTTCCCAAGGCCGCTCAAGAAGTCATTCGGAGCGTCGACCTCATTGTAGGCGACGGTGGCCCTGGCTCAGTCAAGAAAATCAACTTCGCCGACGGTAAAAAAATAGCACTTGTTTTATTGTAACACTAAACTCAGATATATAATACAATTTTGCATCAAACGGCGATCTACAAAGGACAATTATATTTGGTAATTATCAGCCTAACCCAAAAGCTTAAAGCTTAAGTTGATTGATTGCAAATGTGGGTGCAGGCAGGAAGTATAATTTCTTAATCAAAAGAGTGGAGGTAGTGGACTACGAGAAGTTCATATACAAGTTCACCATAATCGATGGCGATATGATCGGAGAACAGATTGAGAAGATTGAGACAGAGATCAAGATTTCAGAGTTGCCGGGGGGAGGGTCCATCTTGGAGGGCTTCAGCCAATATTACACCAAAGACCATGTCTTGTTCAACTATGATGAGATCAAGGAGGGGAAACAAAACTCTGCAGGACTTTTTAAGTACATTGAGTCCTATCTCGTGGCCAACCCTCAAGCCTAA

mRNA sequence

ATGGGTGTTCAAGGTTTTCCCACTCAAGTCATCTCCATCATTCCCCCAGCCAAGGCTTTCAAGGCCCTCGTCCTCGACTTCAACCAACTCGTTCCCAAGGCCGCTCAAGAAGTCATTCGGAGCGTCGACCTCATTGTAGGCGACGGTGGCCCTGGCTCAGTCAAGAAAATCAACTTCGCCGACGGCAGGAAGTATAATTTCTTAATCAAAAGAGTGGAGGTAGTGGACTACGAGAAGTTCATATACAAGTTCACCATAATCGATGGCGATATGATCGGAGAACAGATTGAGAAGATTGAGACAGAGATCAAGATTTCAGAGTTGCCGGGGGGAGGGTCCATCTTGGAGGGCTTCAGCCAATATTACACCAAAGACCATGTCTTGTTCAACTATGATGAGATCAAGGAGGGGAAACAAAACTCTGCAGGACTTTTTAAGTACATTGAGTCCTATCTCGTGGCCAACCCTCAAGCCTAA

Coding sequence (CDS)

ATGGGTGTTCAAGGTTTTCCCACTCAAGTCATCTCCATCATTCCCCCAGCCAAGGCTTTCAAGGCCCTCGTCCTCGACTTCAACCAACTCGTTCCCAAGGCCGCTCAAGAAGTCATTCGGAGCGTCGACCTCATTGTAGGCGACGGTGGCCCTGGCTCAGTCAAGAAAATCAACTTCGCCGACGGCAGGAAGTATAATTTCTTAATCAAAAGAGTGGAGGTAGTGGACTACGAGAAGTTCATATACAAGTTCACCATAATCGATGGCGATATGATCGGAGAACAGATTGAGAAGATTGAGACAGAGATCAAGATTTCAGAGTTGCCGGGGGGAGGGTCCATCTTGGAGGGCTTCAGCCAATATTACACCAAAGACCATGTCTTGTTCAACTATGATGAGATCAAGGAGGGGAAACAAAACTCTGCAGGACTTTTTAAGTACATTGAGTCCTATCTCGTGGCCAACCCTCAAGCCTAA

Protein sequence

MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFADGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQYYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA

Homology

[Close]

BLAST of Lag0015565 vs. NCBI nr
Match: CAD33532.1 (pathogenesis-related protein PR10A [Datisca glomerata])

HSP 1 Score: 181.4 bits (459), Expect = 6.0e-42
Identity = 86/158 (54.43%), Postives = 112/158 (70.89%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + T   S+IPP K FKA VLD +QL+PK   + I+   +I GDGGPGS+KKI F 
Sbjct: 1   MGVFTYVTDATSVIPPPKVFKAFVLDADQLIPKIVPQAIKGTGIIEGDGGPGSIKKITFG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G +Y ++  R++V+D E FIY +TII+GD IG++IEK+E E+KI   P GGSIL+  S+
Sbjct: 61  EGSEYKYMKHRIDVLDKENFIYSYTIIEGDAIGDKIEKVENEVKIEASPTGGSILKSTSK 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           YYTKD      DEIK GK+ + GLFK IESYL+ANP A
Sbjct: 121 YYTKDDYELKEDEIKAGKEKALGLFKAIESYLLANPNA 158

[Close]

BLAST of Lag0015565 vs. NCBI nr
Match: XP_042991726.1 (major allergen Pru ar 1-like [Carya illinoinensis] >KAG2726988.1 hypothetical protein I3760_01G139000 [Carya illinoinensis] >KAG6668040.1 hypothetical protein CIPAW_01G143400 [Carya illinoinensis] >KAG6731697.1 hypothetical protein I3842_01G142000 [Carya illinoinensis])

HSP 1 Score: 158.7 bits (400), Expect = 4.2e-35
Identity = 73/158 (46.20%), Postives = 109/158 (68.99%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + T+  S+IPPAK FKA VLD + LVPK   + I+S ++I GDGGPG++KKINF 
Sbjct: 1   MGVFTYETETTSVIPPAKLFKAFVLDGDNLVPKVVPQAIKSTEIIEGDGGPGTIKKINFG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G ++ ++  +++ +D+    Y +++I+GD +G+ +EKI  EIKI   P GGSIL+  S 
Sbjct: 61  EGSQFKYVKHKIDAIDHANLSYGYSVIEGDALGDVLEKISYEIKIVASPDGGSILKSTSH 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           Y+ K +     +E+K GK+ +AGLFK IE YL+A+P A
Sbjct: 121 YHIKGNHEIKAEEVKAGKEKAAGLFKAIEEYLLAHPDA 158

[Close]

BLAST of Lag0015565 vs. NCBI nr
Match: XP_034925739.1 (major allergen Pru ar 1-like [Populus alba] >KAG6758743.1 hypothetical protein POTOM_035200 [Populus tomentosa] >TKS06533.1 major pollen allergen Bet v 1k family protein [Populus alba])

HSP 1 Score: 158.3 bits (399), Expect = 5.4e-35
Identity = 75/158 (47.47%), Postives = 106/158 (67.09%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV     ++   IPPAK FKA VLD N L+PKA   VI S+ L+ GDGGPGS+K++NF 
Sbjct: 1   MGVITCEKEIALSIPPAKIFKAFVLDGNHLIPKAVPGVIESLSLLEGDGGPGSIKQVNFG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G  Y ++ +R++V+D E  IY++T+I+GD++G + EK+   +K    P GGSI +G S+
Sbjct: 61  EGTGYKYVKERIDVIDKENCIYEYTMIEGDVLGSEFEKVSNVVKFEASPDGGSICKGSSK 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           YYT   +  N +EI   K+   GLFK IE+YL+ANP A
Sbjct: 121 YYTIGDIKVNEEEIDAFKEKQMGLFKAIEAYLLANPDA 158

[Close]

BLAST of Lag0015565 vs. NCBI nr
Match: XP_018857187.2 (major allergen Pru ar 1-like [Juglans regia] >KAF5465416.1 hypothetical protein F2P56_015427 [Juglans regia])

HSP 1 Score: 158.3 bits (399), Expect = 5.4e-35
Identity = 73/158 (46.20%), Postives = 110/158 (69.62%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + T+  S IPPAK FKA +LD + LVPK A + I+S ++I GDGGPG++KKI+FA
Sbjct: 1   MGVLTYETESTSAIPPAKLFKAFILDGDNLVPKVAPQAIKSAEIIEGDGGPGTIKKISFA 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G  + ++  +++ +D+  F Y +++I+GD +G+ +EKI  EIKI   P GGSIL+  S 
Sbjct: 61  EGSHFKYVKHQIDAIDHANFSYGYSVIEGDALGDVLEKITYEIKIVASPDGGSILKSTSH 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           Y+TK       +++K GK+ +AGLFK +E YL+A+P A
Sbjct: 121 YHTKGDHEIKEEDVKAGKEKAAGLFKAVEEYLLAHPDA 158

[Close]

BLAST of Lag0015565 vs. NCBI nr
Match: XP_022761235.1 (major allergen Pru ar 1-like [Durio zibethinus])

HSP 1 Score: 157.9 bits (398), Expect = 7.1e-35
Identity = 73/158 (46.20%), Postives = 108/158 (68.35%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MG+  + T++++ IPPAK F+A VLD ++L+PK   +  +SV+ I GDGGPGS+KKI F 
Sbjct: 1   MGLFTYETEIVTAIPPAKMFQAFVLDGDKLIPKIVPKAFKSVEFIEGDGGPGSIKKITFG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           DG ++N++  +VE VD + F Y +++I+GD +   +EKI  E K+   P GGSI +  S+
Sbjct: 61  DGSRFNYVKHKVEAVDKDNFTYCYSVIEGDALMNTLEKITYETKLEPSPDGGSICKNISK 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           YYT   +    +EIK GK+ + GLFK IE+YL+ANP A
Sbjct: 121 YYTIGDIEIKEEEIKAGKEKALGLFKAIEAYLLANPDA 158

[Close]

BLAST of Lag0015565 vs. ExPASy Swiss-Prot
Match: A0A024B4E4 (Major strawberry allergen Fra a 1.07 OS=Fragaria ananassa OX=3747 GN=Fra a 1.07 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 7.9e-37
Identity = 68/158 (43.04%), Postives = 110/158 (69.62%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + T+  S+IPP + +KA VLD + L+PK A + ++S +L+ GDGG G++KKI+  
Sbjct: 1   MGVFTYETEFTSVIPPPRLYKAFVLDTDNLIPKIAPQAVKSTELVQGDGGVGTIKKIHLG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G +Y+++  +++ +D + F+Y ++II+GD IG+++EKI  EIK+   P GGSI++  S 
Sbjct: 61  EGSEYSYVKHQIDGLDKDNFVYNYSIIEGDAIGDKVEKISYEIKLVASPSGGSIIKSTSH 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           Y+ K  V    + +K GK+ +AGLFK IE+YL+ NP A
Sbjct: 121 YHCKGEVEIKEEHVKAGKERAAGLFKIIENYLLGNPDA 158

[Close]

BLAST of Lag0015565 vs. ExPASy Swiss-Prot
Match: A0A024B2V6 (Major strawberry allergen Fra a 1.08 OS=Fragaria ananassa OX=3747 GN=Fra a 1.08 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.0e-36
Identity = 67/158 (42.41%), Postives = 112/158 (70.89%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + T+  S+IPP + +KA VLD + L+PK A + ++S +++ GDGG G++KKI+  
Sbjct: 1   MGVFTYETEFTSVIPPPRLYKAFVLDADNLIPKIAPQAVKSAEIVQGDGGVGTIKKIHLG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G +Y+++  +++ +D + F+Y ++II+GD IG+++EKI  EIK+   P GGSI++  S 
Sbjct: 61  EGSEYSYVKHQIDGLDKDNFVYNYSIIEGDAIGDKVEKISYEIKLVASPSGGSIIKSTSH 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           Y+ K  V    + +K GK+ +AGLFK IE++L+ANP+A
Sbjct: 121 YHCKGEVEIKEEHVKAGKEKAAGLFKIIENHLLANPEA 158

[Close]

BLAST of Lag0015565 vs. ExPASy Swiss-Prot
Match: D0E0C7 (Major strawberry allergen Fra a 1-3 OS=Fragaria ananassa OX=3747 GN=FRAA3 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 8.7e-36
Identity = 69/157 (43.95%), Postives = 112/157 (71.34%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + ++  S+IPP K FKA VLD + L+PK A + ++S ++I GDGG G++KKI+  
Sbjct: 1   MGVFTYESEFTSVIPPPKLFKAFVLDADNLIPKIAPQAVKSAEIIEGDGGVGTIKKIHLG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G +Y+++  +++ +D + F+Y ++II+GD IG++IEKI  EIK+    GGGSI++  S 
Sbjct: 61  EGSEYSYVKHKIDGIDKDNFVYSYSIIEGDAIGDKIEKISYEIKL-VASGGGSIIKSTSH 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQ 158
           Y+TK  V    + +K GK+ +AGLFK IE++L+A+P+
Sbjct: 121 YHTKGEVEIKEEHVKAGKERAAGLFKIIENHLLAHPE 156

[Close]

BLAST of Lag0015565 vs. ExPASy Swiss-Prot
Match: O50001 (Major allergen Pru ar 1 OS=Prunus armeniaca OX=36596 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 9.7e-35
Identity = 66/158 (41.77%), Postives = 105/158 (66.46%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + T+  S+IPP K FKA +LD + L+PK A   ++  +++ GDGG G++KK+ F 
Sbjct: 1   MGVFTYETEFTSVIPPEKLFKAFILDADNLIPKVAPTAVKGTEILEGDGGVGTIKKVTFG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G +Y ++  RV+ +D +   Y +T+I+GD + + IE I  +IK+   P GGSI++  S 
Sbjct: 61  EGSQYAYVKHRVDGIDKDNLSYSYTLIEGDALSDVIENIAYDIKLVASPDGGSIVKTTSH 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           Y+TK  V    +++K GK+ +AGLFK +E+YL+ANP A
Sbjct: 121 YHTKGDVEIKEEQVKAGKEKAAGLFKLVEAYLLANPDA 158

[Close]

BLAST of Lag0015565 vs. ExPASy Swiss-Prot
Match: O24248 (Major allergen Pru av 1 OS=Prunus avium OX=42229 GN=PRUA1 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.2e-34
Identity = 66/158 (41.77%), Postives = 105/158 (66.46%), Query Frame = 0


Query: 1   MGVQGFPTQVISIIPPAKAFKALVLDFNQLVPKAAQEVIRSVDLIVGDGGPGSVKKINFA 60
           MGV  + ++  S IPP + FKA VLD + LVPK A + I+  +++ GDGGPG++KKI F 
Sbjct: 1   MGVFTYESEFTSEIPPPRLFKAFVLDADNLVPKIAPQAIKHSEILEGDGGPGTIKKITFG 60

Query: 61  DGRKYNFLIKRVEVVDYEKFIYKFTIIDGDMIGEQIEKIETEIKISELPGGGSILEGFSQ 120
           +G +Y ++  +++ +D E + Y +T+I+GD +G+ +EKI  E K+   P GGSI++  S 
Sbjct: 61  EGSQYGYVKHKIDSIDKENYSYSYTLIEGDALGDTLEKISYETKLVASPSGGSIIKSTSH 120

Query: 121 YYTKDHVLFNYDEIKEGKQNSAGLFKYIESYLVANPQA 159
           Y+TK +V    + +K GK+ ++ LFK IE+YL  +P A
Sbjct: 121 YHTKGNVEIKEEHVKAGKEKASNLFKLIETYLKGHPDA 158

The following BLAST results are available for this feature:

BLAST of Lag0015565 vs. NCBI nr


Match Name	E-value	Identity	Description
CAD33532.1	6.0e-42	54.43	pathogenesis-related protein PR10A [Datisca glomerata]	[more]
XP_042991726.1	4.2e-35	46.20	major allergen Pru ar 1-like [Carya illinoinensis] >KAG2726988.1 hypothetical pr...	[more]
XP_034925739.1	5.4e-35	47.47	major allergen Pru ar 1-like [Populus alba] >KAG6758743.1 hypothetical protein P...	[more]
XP_018857187.2	5.4e-35	46.20	major allergen Pru ar 1-like [Juglans regia] >KAF5465416.1 hypothetical protein ...	[more]
XP_022761235.1	7.1e-35	46.20	major allergen Pru ar 1-like [Durio zibethinus]	[more]

BLAST of Lag0015565 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A024B4E4	7.9e-37	43.04	Major strawberry allergen Fra a 1.07 OS=Fragaria ananassa OX=3747 GN=Fra a 1.07 ...	[more]
A0A024B2V6	1.0e-36	42.41	Major strawberry allergen Fra a 1.08 OS=Fragaria ananassa OX=3747 GN=Fra a 1.08 ...	[more]
D0E0C7	8.7e-36	43.95	Major strawberry allergen Fra a 1-3 OS=Fragaria ananassa OX=3747 GN=FRAA3 PE=1 S...	[more]
O50001	9.7e-35	41.77	Major allergen Pru ar 1 OS=Prunus armeniaca OX=36596 PE=1 SV=1	[more]
O24248	2.2e-34	41.77	Major allergen Pru av 1 OS=Prunus avium OX=42229 GN=PRUA1 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024949	Bet v I type allergen	PRINTS	PR00634	BETALLERGEN	coord: 26..36 score: 43.13 coord: 85..98 score: 39.51 coord: 50..59 score: 61.6 coord: 3..23 score: 37.13 coord: 110..126 score: 39.74 coord: 66..85 score: 27.55 coord: 144..154 score: 58.8
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 3..154 e-value: 9.0E-17 score: 61.4
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 2..158 e-value: 7.1E-45 score: 154.8
None	No IPR available	PANTHER	PTHR31213	FAMILY NOT NAMED	coord: 2..158
None	No IPR available	PANTHER	PTHR31213:SF93	MAJOR ALLERGEN PRU AV 1-LIKE	coord: 2..158
None	No IPR available	CDD	cd07816	Bet_v1-like	coord: 14..152 e-value: 3.52208E-38 score: 125.38
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 10..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015565.1	Lag0015565.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0006952	defense response
biological_process	GO:0080163	regulation of protein serine/threonine phosphatase activity
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0010427	abscisic acid binding
molecular_function	GO:0004864	protein phosphatase inhibitor activity
molecular_function	GO:0038023	signaling receptor activity