Lag0015371 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0015371
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAluminum-activated malate transporter
Locationchr12: 11771452 .. 11774576 (+)
RNA-Seq ExpressionLag0015371
SyntenyLag0015371
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAAGCAGGAAATTTGGAATGGAGGGTGAATGTGGGTGATGGGACAAGAGAGATTTTAAGGCCAGAAGTCCCATTTTTGCAGAAAATGGGGATGAGGGTGAAGGGTTTTATTTGGGGATTGTTTGTGAAGGCTTGGAAGTTCTTGGAAAAGGCTTGGGGAATAGCAAATTCTGAGCCAAAGAAGGCTGTTCATGGGCTTAAAGTGGGGCTGGCTCTGACCCTTGTGTCCATCTTTTACTACATGAGACCTTTGTATGAAGGAGTTGGAGGGAATGCCATGTGGGCCATTATGACTGTTGTTGTCACTTTTGAATCCACTGTGGGTTAGTTTTCCCAACCCCCCTTTTTCCTCTTTATAAGCTTATCCAATTTTATCCTTTTTTAACTTGAGTTAGAATAATTTAACATTATTGGAGTCTACATAGGAGTGTTATATAATATAGATTAAGATAAAATTATCATTTTAGACTAAAATATTTGGATACTTGCAATTTTTTCCATATTATTTTAAAAGTTTCTATTAAGTCTTTTTAATTAAAGTTTTGATTAAATCTCTTTGAAGATCTCCACCCTAATTGAAGATTAAAAACTTTCTTGTGGAAGACAATTTTTTAAACATCGTTTATATATACCATAGCGATCAAGAGGTTTTGGAGATTTAATCAAATCATAGAAACGTAGGATGGAATTGAAAGCTTGAGATTAAAATGATGATTTAACCAAATATATTAAATACAAATCACAAACCATTAAGTTGAGTTTTTGAATCTAATAGTAATTTAATTGTACGGACGTTGCTCCACTTTGCAGGCGCGACGTTTTACAAATGTGTAAATAGAATAATAGGAACATCTCTTGCTGGGTGTTTGGGCATTGGAGTTCATTGGGTTGCAGCTGAATCTGGCGATAGATTTGAGCCAATTATTCTTGGGATCTCACTTTTTCTTCTAGGTAAACCATTCTTCCAACATTTCTGGTCTCTCCAAAACTTTCACGAATTTCATTACAACAAACTCTCATATTGGCTCAATGTTTTCATGCATGCATGCAGCTTCAGCAGCCACTTTCTCTCGGTTCATACCATCAGTGAAGACGAGATTCGACTATGGCGCCATGATCTTCATCCTCACCTTCTGTTTGGTTTCGATCTCTGGTTATCGAGTCGATAAGGTGTTCGAGCTGGCCCGTACTCGGCTCAGTACCATCGCCATTGGGACCTCGCTGTGTATTTTCGTCAGCATGCTTTTCTGCCCCATGTGGGCTGGCAGTCAGCTTCATAGCCTCATTTCCCGCAACTTGGATAAACTTGCCCATGCCTTGGATGGTAAATTCCTATTCCTTCCTAGTTTAGTACGATTTTTAAAATTAAAATAAACTACAAGGGAAATGCCTTTTATCCTTCTTTCTTCGCTTTGCCCTTTCGTCTATTTATTAACTCATACATATGTATTTACCTTGATGATATTCTTCTTCAATTCAGGTTGTGTTTCTGAGTACTTCAAGAACGATGATGATCCAGAGATGAAAAATGAAGATCAGGAGGGTCACGATTCCAAAGTTGAAGGCTATAAATGTGTGCTTAATTCAAAGGCATCAGAAGAATCTATGGTAAATAAATAAAGACTCTCTTTTAATTTCTTGGCCAACACTCTAATAAAATGTTATCAAAAACTATGAACTATCATTATGGCTTAATATGGTACCAAAACAAGATGTCTTCGATTGATATAAATAGCTTAGGTATTGAGCTGCTTAAAACAATTAATGGCATGTTTAGCTCAAATTCAAAATCACTTTACAAGCATTTAATCAATTTAAAACCAATTTTAATGGTATGTAAGTTGTGTTCAAAAGGATAAAATCAAATATTAAATTTATGTTTGAGAGTTGTTTTGAATATAACAAAAGGGATTTTAACCCATTTCAAAATCACTTCCAAATATGTCCTATGTTTCTGAGCTCATACAAGTACTCTTAACAATATATATAAAAGATTTAATACATCATAGACCTTTCAATATTAATTTAAGATTCTAAATCCAATAGTAGTTTATCAACCACTAGGATTTCAAGATTAAATATTTGAACCAACACCATATCATACCTTATTATTTTAAGTTATTGGGTTGAATAATAGTTTAATGTATCATAAGATATCTTATCATTTTAAAGCTACTTTTTCTGGATGATCTCTTAAGCTTTTAGCTCTAACAAATGTGGTACTTTTGTAACTTGCAGGCTAACTTTGCAAGATGGGAACCTGCACATGGGCGTTTTGGTTTCAGACACCCATGGAAAAAATATCTTGAGATTGGAGTAGTAATGCGCAAATGTGCATATTGCATTGAGGCTCTTCATGGCTGCCTAAACTCAGAGATTAAGGTCAATTTTCTAATATAAATCCTATCTTTTTATGAGCCCATTTTTCAAAATAGTTAAACTTACCTCATAACCGGTCTAGATGAATCTTCCGACTGATCATCACTAAAATTTTGATCGACATCTATTAAAAAGAAAAAAGTTTCTCTTCTGATCTCTTCCTGTTTGCGTTCTTCTTCTTACTTGTCAAAAACATAATGGTAGTCAACAGAAGATTCCAATTGACCAATTAAAGGTAAATTTTGTCACATTTTATCTAATTCTCTTGCTTTTTGGACACTCATGCAGGCACCAGACTCTTTAAAAATGCATTTGGCTGAACCTTGCAAGGCACTGAGCAGCTCTTCATCCAAAGTCTTGAAGGAACTATCTGTTGTGATCAAGAAGATGAAAAAATCAACAAAGATTGACTTGGAGGTATCTGATATGAACATTGCTGTGCAAGAACTTCAAAATGCCATAAAGTCACTTCCAAGCCCTCAAATGGGAGTCTCATCAGCAGAGCCAGATCAGGGGGCCAATCAAGATCCTAAACCAACAATTCCATCTCTCATGGAGCTTCTTCCACTGGCCACATTGGTGTCACTATTGATAGAAACTGTGTCGAGAATCGAACACGTCGTGAACGCAGTCGAAGCGTTAGCAGATGTTGCTAATTTCGATACCGAAGAGGAGAAGAAGAAGCCACCTTCAGATAACCATAATCATCATGTAGCCATGAGGGTCTTTCCAGAGGCTTAA

mRNA sequence

ATGGCAAAAGCAGGAAATTTGGAATGGAGGGTGAATGTGGGTGATGGGACAAGAGAGATTTTAAGGCCAGAAGTCCCATTTTTGCAGAAAATGGGGATGAGGGTGAAGGGTTTTATTTGGGGATTGTTTGTGAAGGCTTGGAAGTTCTTGGAAAAGGCTTGGGGAATAGCAAATTCTGAGCCAAAGAAGGCTGTTCATGGGCTTAAAGTGGGGCTGGCTCTGACCCTTGTGTCCATCTTTTACTACATGAGACCTTTGTATGAAGGAGTTGGAGGGAATGCCATGTGGGCCATTATGACTGTTGTTGTCACTTTTGAATCCACTGTGGGCGCGACGTTTTACAAATGTGTAAATAGAATAATAGGAACATCTCTTGCTGGGTGTTTGGGCATTGGAGTTCATTGGGTTGCAGCTGAATCTGGCGATAGATTTGAGCCAATTATTCTTGGGATCTCACTTTTTCTTCTAGCTTCAGCAGCCACTTTCTCTCGGTTCATACCATCAGTGAAGACGAGATTCGACTATGGCGCCATGATCTTCATCCTCACCTTCTGTTTGGTTTCGATCTCTGGTTATCGAGTCGATAAGGTGTTCGAGCTGGCCCGTACTCGGCTCAGTACCATCGCCATTGGGACCTCGCTGTGTATTTTCGTCAGCATGCTTTTCTGCCCCATGTGGGCTGGCAGTCAGCTTCATAGCCTCATTTCCCGCAACTTGGATAAACTTGCCCATGCCTTGGATGGTTGTGTTTCTGAGTACTTCAAGAACGATGATGATCCAGAGATGAAAAATGAAGATCAGGAGGGTCACGATTCCAAAGTTGAAGGCTATAAATGTGTGCTTAATTCAAAGGCATCAGAAGAATCTATGGCTAACTTTGCAAGATGGGAACCTGCACATGGGCGTTTTGGTTTCAGACACCCATGGAAAAAATATCTTGAGATTGGAGTAGTAATGCGCAAATGTGCATATTGCATTGAGGCTCTTCATGGCTGCCTAAACTCAGAGATTAAGGCACCAGACTCTTTAAAAATGCATTTGGCTGAACCTTGCAAGGCACTGAGCAGCTCTTCATCCAAAGTCTTGAAGGAACTATCTGTTGTGATCAAGAAGATGAAAAAATCAACAAAGATTGACTTGGAGGTATCTGATATGAACATTGCTGTGCAAGAACTTCAAAATGCCATAAAGTCACTTCCAAGCCCTCAAATGGGAGTCTCATCAGCAGAGCCAGATCAGGGGGCCAATCAAGATCCTAAACCAACAATTCCATCTCTCATGGAGCTTCTTCCACTGGCCACATTGGTGTCACTATTGATAGAAACTGTGTCGAGAATCGAACACGTCGTGAACGCAGTCGAAGCGTTAGCAGATGTTGCTAATTTCGATACCGAAGAGGAGAAGAAGAAGCCACCTTCAGATAACCATAATCATCATGTAGCCATGAGGGTCTTTCCAGAGGCTTAA

Coding sequence (CDS)

ATGGCAAAAGCAGGAAATTTGGAATGGAGGGTGAATGTGGGTGATGGGACAAGAGAGATTTTAAGGCCAGAAGTCCCATTTTTGCAGAAAATGGGGATGAGGGTGAAGGGTTTTATTTGGGGATTGTTTGTGAAGGCTTGGAAGTTCTTGGAAAAGGCTTGGGGAATAGCAAATTCTGAGCCAAAGAAGGCTGTTCATGGGCTTAAAGTGGGGCTGGCTCTGACCCTTGTGTCCATCTTTTACTACATGAGACCTTTGTATGAAGGAGTTGGAGGGAATGCCATGTGGGCCATTATGACTGTTGTTGTCACTTTTGAATCCACTGTGGGCGCGACGTTTTACAAATGTGTAAATAGAATAATAGGAACATCTCTTGCTGGGTGTTTGGGCATTGGAGTTCATTGGGTTGCAGCTGAATCTGGCGATAGATTTGAGCCAATTATTCTTGGGATCTCACTTTTTCTTCTAGCTTCAGCAGCCACTTTCTCTCGGTTCATACCATCAGTGAAGACGAGATTCGACTATGGCGCCATGATCTTCATCCTCACCTTCTGTTTGGTTTCGATCTCTGGTTATCGAGTCGATAAGGTGTTCGAGCTGGCCCGTACTCGGCTCAGTACCATCGCCATTGGGACCTCGCTGTGTATTTTCGTCAGCATGCTTTTCTGCCCCATGTGGGCTGGCAGTCAGCTTCATAGCCTCATTTCCCGCAACTTGGATAAACTTGCCCATGCCTTGGATGGTTGTGTTTCTGAGTACTTCAAGAACGATGATGATCCAGAGATGAAAAATGAAGATCAGGAGGGTCACGATTCCAAAGTTGAAGGCTATAAATGTGTGCTTAATTCAAAGGCATCAGAAGAATCTATGGCTAACTTTGCAAGATGGGAACCTGCACATGGGCGTTTTGGTTTCAGACACCCATGGAAAAAATATCTTGAGATTGGAGTAGTAATGCGCAAATGTGCATATTGCATTGAGGCTCTTCATGGCTGCCTAAACTCAGAGATTAAGGCACCAGACTCTTTAAAAATGCATTTGGCTGAACCTTGCAAGGCACTGAGCAGCTCTTCATCCAAAGTCTTGAAGGAACTATCTGTTGTGATCAAGAAGATGAAAAAATCAACAAAGATTGACTTGGAGGTATCTGATATGAACATTGCTGTGCAAGAACTTCAAAATGCCATAAAGTCACTTCCAAGCCCTCAAATGGGAGTCTCATCAGCAGAGCCAGATCAGGGGGCCAATCAAGATCCTAAACCAACAATTCCATCTCTCATGGAGCTTCTTCCACTGGCCACATTGGTGTCACTATTGATAGAAACTGTGTCGAGAATCGAACACGTCGTGAACGCAGTCGAAGCGTTAGCAGATGTTGCTAATTTCGATACCGAAGAGGAGAAGAAGAAGCCACCTTCAGATAACCATAATCATCATGTAGCCATGAGGGTCTTTCCAGAGGCTTAA

Protein sequence

MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLDKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHHVAMRVFPEA
Homology
BLAST of Lag0015371 vs. NCBI nr
Match: XP_038906852.1 (aluminum-activated malate transporter 10 [Benincasa hispida])

HSP 1 Score: 818.5 bits (2113), Expect = 3.0e-233
Identity = 414/489 (84.66%), Postives = 450/489 (92.02%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWG-LFVKAWKFLEKAWGIANS 60
           MA +GNLEWRVN+GDGT EILRPE   L K+ +R+KGFIWG LFVK WKFLEKAWGIANS
Sbjct: 1   MASSGNLEWRVNMGDGTTEILRPEASILGKIMLRIKGFIWGALFVKPWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKKA+HGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKAIHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 IIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMI 180
           +IGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLASA TFSRFIPSVK+RFDYGAMI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASATTFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSIS YRV+K+FELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL +L +RNL
Sbjct: 181 FVLTFCLVSISSYRVNKLFELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQTLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPA 300
           DKLAH+LDGCVSEYF+NDD PEMKNE+QE H SKVEGYKCVLNSKASEESMANFARWEPA
Sbjct: 241 DKLAHSLDGCVSEYFENDDTPEMKNEEQENHSSKVEGYKCVLNSKASEESMANFARWEPA 300

Query: 301 HGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSS 360
           HGRFGFRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCK LSSSSS
Sbjct: 301 HGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKELSSSSS 360

Query: 361 KVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQDP 420
           KVLKELS+VIKKMKKSTKID  VSDMNIA+QELQNAIKSL  PQM VS +E ++  N + 
Sbjct: 361 KVLKELSIVIKKMKKSTKIDFLVSDMNIALQELQNAIKSL--PQMEVSLSEQEE--NNEK 420

Query: 421 KPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHH 480
               P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKKKP  DNH+H+
Sbjct: 421 ATITPPLMELLPLATLVSLLIETTSRIEHVVNAVETLANVANFDSEEEKKKPSLDNHDHN 480

Query: 481 VAMRVFPEA 489
           VAMRVFP+A
Sbjct: 481 VAMRVFPQA 485

BLAST of Lag0015371 vs. NCBI nr
Match: XP_011658401.1 (aluminum-activated malate transporter 10 [Cucumis sativus] >KGN46529.1 hypothetical protein Csa_005000 [Cucumis sativus])

HSP 1 Score: 806.2 bits (2081), Expect = 1.5e-229
Identity = 410/496 (82.66%), Postives = 452/496 (91.13%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           M   GNLEWRVN+GDGT EILRPE  F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MTSNGNLEWRVNMGDGTTEILRPEASFMKNIMMRIKNFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKKAVHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKAVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 IIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMI 180
           +IGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLAS  TFSRFIPSVK+RFDYGAMI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASVTTFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+K+FELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLFELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDPE--MKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWE 300
           DKLAH+LDGCVSEYF+NDDD E    NED++ ++SKVEGYKCVLNSKASEESMANFARWE
Sbjct: 241 DKLAHSLDGCVSEYFENDDDTEEMKNNEDEKNNNSKVEGYKCVLNSKASEESMANFARWE 300

Query: 301 PAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSS 360
           PAHGRFGFRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSS
Sbjct: 301 PAHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSS 360

Query: 361 SSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQ 420
           SS+VLKELS+VIKKMKKSTKID  VS+MN+AVQELQNAIKS PS QM VS +E ++ AN 
Sbjct: 361 SSEVLKELSIVIKKMKKSTKIDFLVSNMNVAVQELQNAIKSFPSTQMEVSLSEQEEEANN 420

Query: 421 D----PKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP-P 480
           +       TIP LM+LLPLATLVSLLIET SRIEHVVNAVE LA+VAN+D+E+EKKKP  
Sbjct: 421 EDHKAATTTIPPLMKLLPLATLVSLLIETTSRIEHVVNAVETLANVANYDSEDEKKKPSS 480

Query: 481 SDNHNHHVAMRVFPEA 489
           SDNH+H+VAMRVFPEA
Sbjct: 481 SDNHDHNVAMRVFPEA 496

BLAST of Lag0015371 vs. NCBI nr
Match: XP_008459754.1 (PREDICTED: aluminum-activated malate transporter 10 [Cucumis melo])

HSP 1 Score: 803.9 bits (2075), Expect = 7.6e-229
Identity = 415/498 (83.33%), Postives = 454/498 (91.16%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           MA  GNLEWRVN+GDGT EILRP+  F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MASNGNLEWRVNMGDGTTEILRPQASFMKNIMMRIKDFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKK VHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKVVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 IIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMI 180
           +IGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLASAATFSRFIPSVK+RFDYGAMI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASAATFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+K+ ELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLLELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDP-EMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEP 300
           DKLA +LDGCV EYF+NDDD  EMKNEDQ+ ++SKVEGYKCVLNSKASEESMANFARWEP
Sbjct: 241 DKLALSLDGCVCEYFENDDDTGEMKNEDQKNNNSKVEGYKCVLNSKASEESMANFARWEP 300

Query: 301 AHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSS 360
           AHGRFGFRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSSS
Sbjct: 301 AHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSSS 360

Query: 361 SKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPD-QGANQ 420
           S+VLKELS+VIKKMKKSTKID  VS+MNIAVQELQNAIKSLPS QM VS +E D Q AN 
Sbjct: 361 SQVLKELSIVIKKMKKSTKIDFLVSNMNIAVQELQNAIKSLPSTQMEVSLSEQDHQEANN 420

Query: 421 D------PKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP 480
           +         TIP LMELLPLATLVSLLIET SRIEHVVNAVE LA+VA++D+E+EKKKP
Sbjct: 421 EDHHKATTAATIPPLMELLPLATLVSLLIETTSRIEHVVNAVETLANVADYDSEDEKKKP 480

Query: 481 -PSDNHNHHVAMRVFPEA 489
             SDNH+H+VAMRVFPEA
Sbjct: 481 SSSDNHDHNVAMRVFPEA 498

BLAST of Lag0015371 vs. NCBI nr
Match: XP_022960200.1 (aluminum-activated malate transporter 10 [Cucurbita moschata])

HSP 1 Score: 797.3 bits (2058), Expect = 7.1e-227
Identity = 406/489 (83.03%), Postives = 437/489 (89.37%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRPE  F+ +M M VKGF+WGLFVK W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVGRMVMGVKGFVWGLFVKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRI 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFESTVGATFYKCVNR+
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPIILGISLFLLASA TFSRFIPS+KT+FDYGAMIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIILGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDK+FELA TRL+TIAIGTSLCI VSMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIVSMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGCVSEYFKN DDPEM+NE+QE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCVSEYFKNVDDPEMENENQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRFGFRHPWKKYLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKKYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLVEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQDPK 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS Q           AN++ +
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQT----------ANEEHE 420

Query: 421 -PTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHH 480
             TIP LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN++HH
Sbjct: 421 AATIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSSLDNNDHH 479

Query: 481 VAMRVFPEA 489
           VAMRVFPEA
Sbjct: 481 VAMRVFPEA 479

BLAST of Lag0015371 vs. NCBI nr
Match: XP_023513501.1 (aluminum-activated malate transporter 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796.6 bits (2056), Expect = 1.2e-226
Identity = 405/489 (82.82%), Postives = 437/489 (89.37%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRPE  F+ +M M VKGF+WGLFVK W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVGRMVMGVKGFVWGLFVKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRI 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFESTVGATFYKCVNR+
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPI+LGISLFLLASA TFSRFIPS+KT+FDYGAMIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIVLGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDK+FELA TRL+TIAIGTSLCI VSMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIVSMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGC+SEYFKN DDPEM+NEDQE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCLSEYFKNVDDPEMENEDQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRFGFRHPWKKYLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKKYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLLEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQDPK 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS Q           AN++ +
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQT----------ANEEHE 420

Query: 421 -PTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHH 480
             TIP LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN++HH
Sbjct: 421 AATIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSCLDNNDHH 479

Query: 481 VAMRVFPEA 489
           VAMRVFPEA
Sbjct: 481 VAMRVFPEA 479

BLAST of Lag0015371 vs. ExPASy Swiss-Prot
Match: O23086 (Aluminum-activated malate transporter 10 OS=Arabidopsis thaliana OX=3702 GN=ALMT10 PE=3 SV=2)

HSP 1 Score: 514.2 bits (1323), Expect = 1.6e-144
Identity = 272/489 (55.62%), Postives = 356/489 (72.80%), Query Frame = 0

Query: 3   KAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFV-KAWKFLEKAWGIANSEP 62
           +AG LEWR++V +GT E L P     +++ + +K  +  + + +  KF+ KAW I   +P
Sbjct: 5   EAGKLEWRISVDNGTTERLVPRSGLSKRIFLWLKDLVMKVIMERVAKFMRKAWRIGADDP 64

Query: 63  KKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRII 122
            K VH LKVGLAL+LVSIFYYMRPLY+GVGGNAMWAIMTVVV FES VGATF KCVNR++
Sbjct: 65  AKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNAMWAIMTVVVVFESNVGATFCKCVNRVV 124

Query: 123 GTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIFI 182
            T LAG LGI VHWVA +SG + E  ++G S+FL A AAT+SRF+PS K RFDYGAMIFI
Sbjct: 125 ATILAGSLGIAVHWVATQSG-KAEVFVIGCSVFLFAFAATYSRFVPSFKARFDYGAMIFI 184

Query: 183 LTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLDK 242
           LTF LVS+ GYRVDK+ ELA+ R+STIAIGTS+CI +++ FCP+WAGSQLH LI RNL+K
Sbjct: 185 LTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSICIIITVFFCPIWAGSQLHRLIERNLEK 244

Query: 243 LAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESM----------- 302
           LA +LDGCV+EYFK ++    +NED E  + K++G+KCVLNSK +EE+M           
Sbjct: 245 LADSLDGCVAEYFKENEVSTNRNED-ENTNMKLQGFKCVLNSKGTEEAMPLIRFSGFSFS 304

Query: 303 -ANFARWEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAE 362
            AN ARWEPAHG F FRHPWK Y++IG  MR+CAYC+E L  C+N E +APD +K H  E
Sbjct: 305 QANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCAYCLENLSICINYETEAPDQVKNHFGE 364

Query: 363 PCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLP--SPQMGVS 422
            C  LSS+SSK+L+EL+ ++K  +KS+K+D  V DMN AVQELQ  +K++P  + +    
Sbjct: 365 ACMKLSSASSKILRELADMMKNTRKSSKMDFLVFDMNSAVQELQETLKNVPIETNKPEEV 424

Query: 423 SAEPDQGANQDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEE 477
            +E +   + + + T  SL E+LP+ATLVSLLIE  +RI+  V AV+ LA++A+F  E++
Sbjct: 425 PSEEENKVDSEERTTSMSLHEVLPVATLVSLLIENAARIQTAVEAVDELANLADF--EQD 484

BLAST of Lag0015371 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 5.2e-95
Identity = 187/420 (44.52%), Postives = 269/420 (64.05%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +   +P++ VH  KVGLAL LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NR + T +AG LGIG H +A+ SG   EPI+L I +F+LA+ +TF RF P VK R+DY
Sbjct: 74  GLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFPRVKARYDY 133

Query: 176 GAMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
           G +IFILTF L+S+SG+R D++ +LA  RLST+ +G   C+ +S+  CP+WAG  LHSL+
Sbjct: 134 GVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWAGQDLHSLL 193

Query: 236 SRNLDKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFAR 295
           + N D L+H L     EYF+  +D ++K  + E     +E YK VLNSK++EE++ANFA+
Sbjct: 194 ASNFDTLSHFLQEFGDEYFEATEDGDIK--EVEKRRRNLERYKSVLNSKSNEEALANFAK 253

Query: 296 WEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALS 355
           WEP HG+F FRHPW++YL +G ++R+ AY I+AL+  +NS+++ P  +K  + EP + +S
Sbjct: 254 WEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKKIEEPLRRMS 313

Query: 356 SSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGA 415
           S S K +KE+S+ +K M  S+  D+ V +   A + L   +KS               G 
Sbjct: 314 SESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKS---------------GI 373

Query: 416 NQDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDN 475
             D +P     ++++ L T VSLLI+ V+  E +  +V  LA  A F   + KKKP   N
Sbjct: 374 LNDVEP-----LQMISLMTTVSLLIDIVNLTEKISESVHELASAAKF---KNKKKPSKSN 408

BLAST of Lag0015371 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 6.1e-88
Identity = 181/413 (43.83%), Postives = 260/413 (62.95%), Query Frame = 0

Query: 57  ANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKC 116
           A  +P++ +H +KVG+ALTLVS+ YY+RPLY   G   MWAI+TVVV FE TVG T  K 
Sbjct: 38  AKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTLSKG 97

Query: 117 VNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYG 176
           +NR   T +AG LG+G   +A   G + EPI+LGI +F L +AATFSRF P +K R+DYG
Sbjct: 98  LNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRYDYG 157

Query: 177 AMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLIS 236
           A+IFILTF  V+ISGYR D++  +A  RLSTI IG ++CI VS+  CP+WAG  LH +I+
Sbjct: 158 ALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHKMIA 217

Query: 237 RNLDKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARW 296
            N++KLA  L+G   EYF+    PE  +++     S V  YK +L SK++E+S+AN ARW
Sbjct: 218 NNINKLAKYLEGFEGEYFQ----PEKISKET---SSCVREYKSILTSKSTEDSLANLARW 277

Query: 297 EPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSS 356
           EP HGRF  RHPWKKYL+I  ++R+CA  +E L+G + S  KAP   +  + EP   +S 
Sbjct: 278 EPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSNDKAPQEFESKIQEPITTMSR 337

Query: 357 SSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGAN 416
              + LK ++  IK M+  +      + +N  +   + AIK+L   ++ + S+ P+    
Sbjct: 338 EVGEALKAIAKSIKTMRNDS------ACVNAHIDNSKKAIKNL---KIALKSSYPE---- 397

Query: 417 QDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKK 470
                T   L+E++P  T+ S+LIE V+ +E +  AVE  + +A+F    + K
Sbjct: 398 -----TYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKETLDSK 425

BLAST of Lag0015371 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.4e-87
Identity = 185/442 (41.86%), Postives = 269/442 (60.86%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMR---PLYEGVGGNAMWAIMTVVVTFESTVGAT 115
           + N +P++ +H  KVGLAL LVS FYY +   P  +  G NAMWA+MTVVV FE +VGAT
Sbjct: 14  VGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGAT 73

Query: 116 FYKCVNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTR 175
             K +NR + T +AG LGIG H +A  SG   EPI+L + +F+ A+ +TF RF P VKT+
Sbjct: 74  LGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKTK 133

Query: 176 FDYGAMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLH 235
           FDYG +IFILTF L+S+SG+R +++ +LA +RLST+ IG   CI +S+  CP+WAG  LH
Sbjct: 134 FDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDLH 193

Query: 236 SLISRNLDKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMAN 295
           SL++ N D L+H L     EYF+  +  + K    E     +E YK VL+SK+ EE++AN
Sbjct: 194 SLLASNFDTLSHFLQDFGDEYFEAREKGDYK--VVEKRKKNLERYKSVLDSKSDEEALAN 253

Query: 296 FARWEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCK 355
           +A WEP HG+F FRHPWK+Y+ +G ++R+CAY I+AL+  +NS+ + P  +K  L  P +
Sbjct: 254 YAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLR 313

Query: 356 ALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPD 415
            +SS S   +KE+S+ +K+M KS+  D+ VS+   A + L   +KS              
Sbjct: 314 RMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKS-------------- 373

Query: 416 QGANQDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANF---------- 475
            G   D +P     ++++ L T VS+LI+ V+  E +  +V  LA  A F          
Sbjct: 374 -GILNDVEP-----LQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPTVLY 433

Query: 476 ---DTEEEKKKPPSDNH-NHHV 481
              D+    +  P D+H +HHV
Sbjct: 434 EKSDSGSIGRAMPIDSHEDHHV 433

BLAST of Lag0015371 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.5e-86
Identity = 180/438 (41.10%), Postives = 259/438 (59.13%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +A  +P++ VH  KVGL L LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NR+  T  AG LGIG H +A+ SG   EPI+L + +F+ A+ +TF RF P VK R+DY
Sbjct: 74  GLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFPRVKARYDY 133

Query: 176 GAMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
             +IFILTF L+S+SG+R ++V +L   R+ST+ IG   C+ +S+  CP+WAG  LHSLI
Sbjct: 134 SLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWAGQDLHSLI 193

Query: 236 SRNLDKLAHALDGCVSEYFKND--------------------------DDPEMKNEDQEG 295
           + N +KL+  L G    Y  +D                          ++   K  D+  
Sbjct: 194 ASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDGAKEVDKRK 253

Query: 296 HDSKVEGYKCVLNSKASEESMANFARWEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEAL 355
            D   + YK VLNSK++EES+ANFA+WEP HG+F FRHPWK+YL +G ++R+CAY I AL
Sbjct: 254 KD--FDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCAYRIHAL 313

Query: 356 HGCLNSEIKAPDSLKMHLAEPCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAV 415
           +  LN++ +    +K  L EP + +S  S K +KE+S+ +KKM K +  DL V +   A 
Sbjct: 314 NSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDLHVQNAKSAC 373

Query: 416 QELQNAIKSLPSPQMGVSSAEPDQGANQDPKPTIPSLMELLPLATLVSLLIETVSRIEHV 468
           + L N + S               G  ++ +P     +EL+ L T +SLLI+ ++  E +
Sbjct: 374 KSLTNLLNS---------------GILKEVEP-----LELVSLLTAISLLIDIINLTEKI 429

BLAST of Lag0015371 vs. ExPASy TrEMBL
Match: A0A0A0KCJ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106810 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 7.4e-230
Identity = 410/496 (82.66%), Postives = 452/496 (91.13%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           M   GNLEWRVN+GDGT EILRPE  F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MTSNGNLEWRVNMGDGTTEILRPEASFMKNIMMRIKNFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKKAVHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKAVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 IIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMI 180
           +IGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLAS  TFSRFIPSVK+RFDYGAMI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASVTTFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+K+FELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLFELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDPE--MKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWE 300
           DKLAH+LDGCVSEYF+NDDD E    NED++ ++SKVEGYKCVLNSKASEESMANFARWE
Sbjct: 241 DKLAHSLDGCVSEYFENDDDTEEMKNNEDEKNNNSKVEGYKCVLNSKASEESMANFARWE 300

Query: 301 PAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSS 360
           PAHGRFGFRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSS
Sbjct: 301 PAHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSS 360

Query: 361 SSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQ 420
           SS+VLKELS+VIKKMKKSTKID  VS+MN+AVQELQNAIKS PS QM VS +E ++ AN 
Sbjct: 361 SSEVLKELSIVIKKMKKSTKIDFLVSNMNVAVQELQNAIKSFPSTQMEVSLSEQEEEANN 420

Query: 421 D----PKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP-P 480
           +       TIP LM+LLPLATLVSLLIET SRIEHVVNAVE LA+VAN+D+E+EKKKP  
Sbjct: 421 EDHKAATTTIPPLMKLLPLATLVSLLIETTSRIEHVVNAVETLANVANYDSEDEKKKPSS 480

Query: 481 SDNHNHHVAMRVFPEA 489
           SDNH+H+VAMRVFPEA
Sbjct: 481 SDNHDHNVAMRVFPEA 496

BLAST of Lag0015371 vs. ExPASy TrEMBL
Match: A0A1S3CB02 (aluminum-activated malate transporter 10 OS=Cucumis melo OX=3656 GN=LOC103498794 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.7e-229
Identity = 415/498 (83.33%), Postives = 454/498 (91.16%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           MA  GNLEWRVN+GDGT EILRP+  F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MASNGNLEWRVNMGDGTTEILRPQASFMKNIMMRIKDFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKK VHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKVVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 IIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMI 180
           +IGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLASAATFSRFIPSVK+RFDYGAMI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASAATFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+K+ ELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLLELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDP-EMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEP 300
           DKLA +LDGCV EYF+NDDD  EMKNEDQ+ ++SKVEGYKCVLNSKASEESMANFARWEP
Sbjct: 241 DKLALSLDGCVCEYFENDDDTGEMKNEDQKNNNSKVEGYKCVLNSKASEESMANFARWEP 300

Query: 301 AHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSS 360
           AHGRFGFRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSSS
Sbjct: 301 AHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSSS 360

Query: 361 SKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPD-QGANQ 420
           S+VLKELS+VIKKMKKSTKID  VS+MNIAVQELQNAIKSLPS QM VS +E D Q AN 
Sbjct: 361 SQVLKELSIVIKKMKKSTKIDFLVSNMNIAVQELQNAIKSLPSTQMEVSLSEQDHQEANN 420

Query: 421 D------PKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP 480
           +         TIP LMELLPLATLVSLLIET SRIEHVVNAVE LA+VA++D+E+EKKKP
Sbjct: 421 EDHHKATTAATIPPLMELLPLATLVSLLIETTSRIEHVVNAVETLANVADYDSEDEKKKP 480

Query: 481 -PSDNHNHHVAMRVFPEA 489
             SDNH+H+VAMRVFPEA
Sbjct: 481 SSSDNHDHNVAMRVFPEA 498

BLAST of Lag0015371 vs. ExPASy TrEMBL
Match: A0A6J1H8F3 (aluminum-activated malate transporter 10 OS=Cucurbita moschata OX=3662 GN=LOC111461009 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.5e-227
Identity = 406/489 (83.03%), Postives = 437/489 (89.37%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRPE  F+ +M M VKGF+WGLFVK W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVGRMVMGVKGFVWGLFVKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRI 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFESTVGATFYKCVNR+
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPIILGISLFLLASA TFSRFIPS+KT+FDYGAMIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIILGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDK+FELA TRL+TIAIGTSLCI VSMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIVSMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGCVSEYFKN DDPEM+NE+QE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCVSEYFKNVDDPEMENENQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRFGFRHPWKKYLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKKYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLVEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQDPK 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS Q           AN++ +
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQT----------ANEEHE 420

Query: 421 -PTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHH 480
             TIP LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN++HH
Sbjct: 421 AATIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSSLDNNDHH 479

Query: 481 VAMRVFPEA 489
           VAMRVFPEA
Sbjct: 481 VAMRVFPEA 479

BLAST of Lag0015371 vs. ExPASy TrEMBL
Match: A0A6J1KS06 (aluminum-activated malate transporter 10 OS=Cucurbita maxima OX=3661 GN=LOC111498132 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.1e-224
Identity = 403/489 (82.41%), Postives = 435/489 (88.96%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRPE  F+ +M M VKGF+WGLF+K W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVSRMVMGVKGFVWGLFMKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRI 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFES+VGATFYKCVNR+
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESSVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPIILGISLFLLASA TFSRFIPS+KT+FDYGAMIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIILGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDK+FELA TRL+TIAIGTSLCI +SMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIISMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGCVSEYFKN DDPEM+NEDQE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCVSEYFKNVDDPEMENEDQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRFGFRHPWK+YLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKQYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLVEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQDPK 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS Q           AN++ +
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQT----------ANEEHE 420

Query: 421 -PTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHH 480
             TIP LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN N H
Sbjct: 421 AATIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSCLDN-NDH 478

Query: 481 VAMRVFPEA 489
           VAMRVFPEA
Sbjct: 481 VAMRVFPEA 478

BLAST of Lag0015371 vs. ExPASy TrEMBL
Match: A0A6J1DX41 (aluminum-activated malate transporter 10 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025265 PE=3 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.4e-204
Identity = 380/492 (77.24%), Postives = 420/492 (85.37%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA AGNLEWRVN+GDGT EIL  E  F  K+ M +K  I  LFVK  KFLEKA GIA SE
Sbjct: 1   MASAGNLEWRVNLGDGTTEILEAEASFGHKLVMGIKSLIMELFVKCRKFLEKAGGIAKSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRI 120
           PKK VHGLKVGLALTLVSIFYYMRPLYEG+GG+AMWAIMTVVVTFE +VGA+ YKCVNR+
Sbjct: 61  PKKGVHGLKVGLALTLVSIFYYMRPLYEGLGGSAMWAIMTVVVTFEYSVGASLYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIF 180
           +GTSLAG LGIGVHWVAAE GDRFEPIILGISLFLLASAATFSRFIPSVK+RFDYGAMIF
Sbjct: 121 VGTSLAGGLGIGVHWVAAECGDRFEPIILGISLFLLASAATFSRFIPSVKSRFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           +LTFCLVSIS YRVD++ ELARTR+ TIA+GT++C+ VSML CP+WAG QLHSL++RN+D
Sbjct: 181 VLTFCLVSISSYRVDELLELARTRILTIAVGTAICLVVSMLVCPVWAGGQLHSLLARNID 240

Query: 241 KLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA A+ GC+SEYFK+ D PEMKNED+E H SK++ YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADAIHGCISEYFKDKDCPEMKNEDREDHASKIQAYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRFGFRHPWK YLE+GV MR+CAYCIEAL+G L+SEIKAP+SLK+HLA PCKALSS  SK
Sbjct: 301 GRFGFRHPWKFYLEVGVAMRRCAYCIEALNGRLDSEIKAPESLKLHLATPCKALSSCCSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGANQDPK 420
           V KELS VIK MKKS KID EVSDMN AVQELQN IKSLP   M VS AEP+ GAN +  
Sbjct: 361 VFKELSTVIKTMKKSNKIDFEVSDMNFAVQELQNTIKSLP---MAVSLAEPN-GANGE-- 420

Query: 421 PTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFD-TEEEKKK--PPSDNH- 480
            TIP LMELL LATLVSLLIET SRIEHVVNAVE LADVA FD  ++EKKK  P SDN  
Sbjct: 421 ATIPPLMELLSLATLVSLLIETASRIEHVVNAVEKLADVAKFDFADDEKKKSNPSSDNRD 480

Query: 481 NHHVAMRVFPEA 489
           +HH AM+VFPEA
Sbjct: 481 HHHAAMKVFPEA 486

BLAST of Lag0015371 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-145
Identity = 272/489 (55.62%), Postives = 356/489 (72.80%), Query Frame = 0

Query: 3   KAGNLEWRVNVGDGTREILRPEVPFLQKMGMRVKGFIWGLFV-KAWKFLEKAWGIANSEP 62
           +AG LEWR++V +GT E L P     +++ + +K  +  + + +  KF+ KAW I   +P
Sbjct: 5   EAGKLEWRISVDNGTTERLVPRSGLSKRIFLWLKDLVMKVIMERVAKFMRKAWRIGADDP 64

Query: 63  KKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRII 122
            K VH LKVGLAL+LVSIFYYMRPLY+GVGGNAMWAIMTVVV FES VGATF KCVNR++
Sbjct: 65  AKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNAMWAIMTVVVVFESNVGATFCKCVNRVV 124

Query: 123 GTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGAMIFI 182
            T LAG LGI VHWVA +SG + E  ++G S+FL A AAT+SRF+PS K RFDYGAMIFI
Sbjct: 125 ATILAGSLGIAVHWVATQSG-KAEVFVIGCSVFLFAFAATYSRFVPSFKARFDYGAMIFI 184

Query: 183 LTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLDK 242
           LTF LVS+ GYRVDK+ ELA+ R+STIAIGTS+CI +++ FCP+WAGSQLH LI RNL+K
Sbjct: 185 LTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSICIIITVFFCPIWAGSQLHRLIERNLEK 244

Query: 243 LAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESM----------- 302
           LA +LDGCV+EYFK ++    +NED E  + K++G+KCVLNSK +EE+M           
Sbjct: 245 LADSLDGCVAEYFKENEVSTNRNED-ENTNMKLQGFKCVLNSKGTEEAMPLIRFSGFSFS 304

Query: 303 -ANFARWEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAE 362
            AN ARWEPAHG F FRHPWK Y++IG  MR+CAYC+E L  C+N E +APD +K H  E
Sbjct: 305 QANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCAYCLENLSICINYETEAPDQVKNHFGE 364

Query: 363 PCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLP--SPQMGVS 422
            C  LSS+SSK+L+EL+ ++K  +KS+K+D  V DMN AVQELQ  +K++P  + +    
Sbjct: 365 ACMKLSSASSKILRELADMMKNTRKSSKMDFLVFDMNSAVQELQETLKNVPIETNKPEEV 424

Query: 423 SAEPDQGANQDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEE 477
            +E +   + + + T  SL E+LP+ATLVSLLIE  +RI+  V AV+ LA++A+F  E++
Sbjct: 425 PSEEENKVDSEERTTSMSLHEVLPVATLVSLLIENAARIQTAVEAVDELANLADF--EQD 484

BLAST of Lag0015371 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 349.7 bits (896), Expect = 3.7e-96
Identity = 187/420 (44.52%), Postives = 269/420 (64.05%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +   +P++ VH  KVGLAL LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NR + T +AG LGIG H +A+ SG   EPI+L I +F+LA+ +TF RF P VK R+DY
Sbjct: 74  GLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFPRVKARYDY 133

Query: 176 GAMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
           G +IFILTF L+S+SG+R D++ +LA  RLST+ +G   C+ +S+  CP+WAG  LHSL+
Sbjct: 134 GVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWAGQDLHSLL 193

Query: 236 SRNLDKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFAR 295
           + N D L+H L     EYF+  +D ++K  + E     +E YK VLNSK++EE++ANFA+
Sbjct: 194 ASNFDTLSHFLQEFGDEYFEATEDGDIK--EVEKRRRNLERYKSVLNSKSNEEALANFAK 253

Query: 296 WEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALS 355
           WEP HG+F FRHPW++YL +G ++R+ AY I+AL+  +NS+++ P  +K  + EP + +S
Sbjct: 254 WEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKKIEEPLRRMS 313

Query: 356 SSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGA 415
           S S K +KE+S+ +K M  S+  D+ V +   A + L   +KS               G 
Sbjct: 314 SESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKS---------------GI 373

Query: 416 NQDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDN 475
             D +P     ++++ L T VSLLI+ V+  E +  +V  LA  A F   + KKKP   N
Sbjct: 374 LNDVEP-----LQMISLMTTVSLLIDIVNLTEKISESVHELASAAKF---KNKKKPSKSN 408

BLAST of Lag0015371 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 326.2 bits (835), Expect = 4.3e-89
Identity = 181/413 (43.83%), Postives = 260/413 (62.95%), Query Frame = 0

Query: 57  ANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKC 116
           A  +P++ +H +KVG+ALTLVS+ YY+RPLY   G   MWAI+TVVV FE TVG T  K 
Sbjct: 38  AKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTLSKG 97

Query: 117 VNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYG 176
           +NR   T +AG LG+G   +A   G + EPI+LGI +F L +AATFSRF P +K R+DYG
Sbjct: 98  LNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRYDYG 157

Query: 177 AMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLIS 236
           A+IFILTF  V+ISGYR D++  +A  RLSTI IG ++CI VS+  CP+WAG  LH +I+
Sbjct: 158 ALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHKMIA 217

Query: 237 RNLDKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMANFARW 296
            N++KLA  L+G   EYF+    PE  +++     S V  YK +L SK++E+S+AN ARW
Sbjct: 218 NNINKLAKYLEGFEGEYFQ----PEKISKET---SSCVREYKSILTSKSTEDSLANLARW 277

Query: 297 EPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSS 356
           EP HGRF  RHPWKKYL+I  ++R+CA  +E L+G + S  KAP   +  + EP   +S 
Sbjct: 278 EPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSNDKAPQEFESKIQEPITTMSR 337

Query: 357 SSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPDQGAN 416
              + LK ++  IK M+  +      + +N  +   + AIK+L   ++ + S+ P+    
Sbjct: 338 EVGEALKAIAKSIKTMRNDS------ACVNAHIDNSKKAIKNL---KIALKSSYPE---- 397

Query: 417 QDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKK 470
                T   L+E++P  T+ S+LIE V+ +E +  AVE  + +A+F    + K
Sbjct: 398 -----TYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKETLDSK 425

BLAST of Lag0015371 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 325.1 bits (832), Expect = 9.7e-89
Identity = 185/442 (41.86%), Postives = 269/442 (60.86%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMR---PLYEGVGGNAMWAIMTVVVTFESTVGAT 115
           + N +P++ +H  KVGLAL LVS FYY +   P  +  G NAMWA+MTVVV FE +VGAT
Sbjct: 14  VGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGAT 73

Query: 116 FYKCVNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTR 175
             K +NR + T +AG LGIG H +A  SG   EPI+L + +F+ A+ +TF RF P VKT+
Sbjct: 74  LGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKTK 133

Query: 176 FDYGAMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLH 235
           FDYG +IFILTF L+S+SG+R +++ +LA +RLST+ IG   CI +S+  CP+WAG  LH
Sbjct: 134 FDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDLH 193

Query: 236 SLISRNLDKLAHALDGCVSEYFKNDDDPEMKNEDQEGHDSKVEGYKCVLNSKASEESMAN 295
           SL++ N D L+H L     EYF+  +  + K    E     +E YK VL+SK+ EE++AN
Sbjct: 194 SLLASNFDTLSHFLQDFGDEYFEAREKGDYK--VVEKRKKNLERYKSVLDSKSDEEALAN 253

Query: 296 FARWEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCK 355
           +A WEP HG+F FRHPWK+Y+ +G ++R+CAY I+AL+  +NS+ + P  +K  L  P +
Sbjct: 254 YAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLR 313

Query: 356 ALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPQMGVSSAEPD 415
            +SS S   +KE+S+ +K+M KS+  D+ VS+   A + L   +KS              
Sbjct: 314 RMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKS-------------- 373

Query: 416 QGANQDPKPTIPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANF---------- 475
            G   D +P     ++++ L T VS+LI+ V+  E +  +V  LA  A F          
Sbjct: 374 -GILNDVEP-----LQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPTVLY 433

Query: 476 ---DTEEEKKKPPSDNH-NHHV 481
              D+    +  P D+H +HHV
Sbjct: 434 EKSDSGSIGRAMPIDSHEDHHV 433

BLAST of Lag0015371 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-87
Identity = 180/438 (41.10%), Postives = 259/438 (59.13%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +A  +P++ VH  KVGL L LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRIIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NR+  T  AG LGIG H +A+ SG   EPI+L + +F+ A+ +TF RF P VK R+DY
Sbjct: 74  GLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFPRVKARYDY 133

Query: 176 GAMIFILTFCLVSISGYRVDKVFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
             +IFILTF L+S+SG+R ++V +L   R+ST+ IG   C+ +S+  CP+WAG  LHSLI
Sbjct: 134 SLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWAGQDLHSLI 193

Query: 236 SRNLDKLAHALDGCVSEYFKND--------------------------DDPEMKNEDQEG 295
           + N +KL+  L G    Y  +D                          ++   K  D+  
Sbjct: 194 ASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDGAKEVDKRK 253

Query: 296 HDSKVEGYKCVLNSKASEESMANFARWEPAHGRFGFRHPWKKYLEIGVVMRKCAYCIEAL 355
            D   + YK VLNSK++EES+ANFA+WEP HG+F FRHPWK+YL +G ++R+CAY I AL
Sbjct: 254 KD--FDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCAYRIHAL 313

Query: 356 HGCLNSEIKAPDSLKMHLAEPCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAV 415
           +  LN++ +    +K  L EP + +S  S K +KE+S+ +KKM K +  DL V +   A 
Sbjct: 314 NSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDLHVQNAKSAC 373

Query: 416 QELQNAIKSLPSPQMGVSSAEPDQGANQDPKPTIPSLMELLPLATLVSLLIETVSRIEHV 468
           + L N + S               G  ++ +P     +EL+ L T +SLLI+ ++  E +
Sbjct: 374 KSLTNLLNS---------------GILKEVEP-----LELVSLLTAISLLIDIINLTEKI 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906852.13.0e-23384.66aluminum-activated malate transporter 10 [Benincasa hispida][more]
XP_011658401.11.5e-22982.66aluminum-activated malate transporter 10 [Cucumis sativus] >KGN46529.1 hypotheti... [more]
XP_008459754.17.6e-22983.33PREDICTED: aluminum-activated malate transporter 10 [Cucumis melo][more]
XP_022960200.17.1e-22783.03aluminum-activated malate transporter 10 [Cucurbita moschata][more]
XP_023513501.11.2e-22682.82aluminum-activated malate transporter 10 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O230861.6e-14455.62Aluminum-activated malate transporter 10 OS=Arabidopsis thaliana OX=3702 GN=ALMT... [more]
Q9SJE85.2e-9544.52Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9SRM96.1e-8843.83Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q9SJE91.4e-8741.86Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q9XIN11.5e-8641.10Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Match NameE-valueIdentityDescription
A0A0A0KCJ27.4e-23082.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106810 PE=3 SV=1[more]
A0A1S3CB023.7e-22983.33aluminum-activated malate transporter 10 OS=Cucumis melo OX=3656 GN=LOC103498794... [more]
A0A6J1H8F33.5e-22783.03aluminum-activated malate transporter 10 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1KS062.1e-22482.41aluminum-activated malate transporter 10 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1DX411.4e-20477.24aluminum-activated malate transporter 10 isoform X1 OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
AT4G00910.11.1e-14555.62Aluminium activated malate transporter family protein [more]
AT1G08440.13.7e-9644.52Aluminium activated malate transporter family protein [more]
AT3G11680.14.3e-8943.83Aluminium activated malate transporter family protein [more]
AT1G08430.19.7e-8941.86aluminum-activated malate transporter 1 [more]
AT2G27240.11.1e-8741.10Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 54..403
e-value: 1.5E-153
score: 512.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..422
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 464..488
NoneNo IPR availablePANTHERPTHR31086:SF4ALUMINUM ACTIVATED MALATE TRANSPORTER FAMILY PROTEINcoord: 5..472
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 5..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0015371.1Lag0015371.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane