Lag0015313 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0015313
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCCHC-type domain-containing protein
Locationchr12: 10177142 .. 10178032 (+)
RNA-Seq ExpressionLag0015313
SyntenyLag0015313
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGTTGTCCGTGTGGGTGCGGTTGTGTAGTGCAAATGTGTGGGTTGTGTGTGGTTCTCAGTTTGTTGTTTAGGTTGTGTTTGGAGTGTGTCTGTGTTCTGCAGTTGCTAGTAGGGTGGGAGGGTGTTAGTGGTGTGTGCATTGGTGTTCTGGGGGTGTGGGGTTGGGTAGTGGTTAAAGCCCTGGTGATAATGGAGGATCTTGTCAGTAGGTAGAATTCTTGGAAGCTAACGGATGAGGAGGCGAAGATGGTTCGGCCAGGGGAGGGAGCATCGGTCCTGTCAGTGGGCTCGCTAGAGTTGTGTGTGGTAGGGAAAGTGTTCTCCTCAAAACGTGTGAATGTGGAGGCTTTTCGGAATGTTATGATGGCAGCGTGGAAAGTTCATGGGGCAACGCGCATAGAATCTGCAGGGGAGAATTTATTTGCTATTCAATTCAGATCCAAGGGGGAAAAGATGCGAGTGATGACTACGGGCCGTGGGCTTTCGATCGTTCTCTTGTGGTCCTAAAGGAGCCGAGGTCCGCAGGGTCGGTATTGGATGTGGAGTTTAATGACTGTGTCTTTTGGGTCCAGATTCACAATGTACCTATGCGCTGGCAGACGAGAGAGATGGCTCAGTCTTTGGGTGGTAAGATTGGTACTGTGGAGAAGTTGGGGTTCAAGGGATGGTGACTGGACGGGTCCGATTCTTCGAAAACAGGTTCTTTGAGGATGGATCGGCCGCTGAGGAGGGGGTTTCGGTTGCAAGATGAAGAGGGTAAAGAATTTCGGTGCCCGGTACTGTATGAGCGGCTGCCAGATTTTTGCTATGATTGTGGTTGTCTGGGGCACTCAAGAAGGGAGTGTGGGGTTGTTGGTGAAGGTGTGTCGGGTCAGCAGGGTGAGTAG

mRNA sequence

ATGTGGTTGTCCGTGTGGTTGCTAGTAGGGTGGGAGGGTGTTAGTGGTGTGTGCATTGGTGTTCTGGGGGTGTGGGGTTGGGTAGTGGTTAAAGCCCTGGTGATAATGGAGGATCTTGTCAGTAGCGTGGAAAGTTCATGGGGCAACGCGCATAGAATCTGCAGGGGAGAATTTATTTGCTATTCAATTCAGATCCAAGGGGGAAAAGATGCGAGTGATGACTACGGGCCGTGGGCTTTCGATCGTTCTCTTGTGGTCCTAAAGGAGCCGAGGTCCGCAGGGTCGGTATTGGATGTGGAGTTTAATGACTGTGTCTTTTGGGTCCAGATTCACAATGTACCTATGCGCTGGCAGACGAGAGAGATGGCTCAGTCTTTGGGTGGTTCTTTGAGGATGGATCGGCCGCTGAGGAGGGGGTTTCGGTTGCAAGATGAAGAGGGTAAAGAATTTCGGTGCCCGGTACTGTATGAGCGGCTGCCAGATTTTTGCTATGATTGTGGTTGTCTGGGGCACTCAAGAAGGGAGTGTGGGGTTGTTGGTGAAGGTGTGTCGGGTCAGCAGGGTGAGTAG

Coding sequence (CDS)

ATGTGGTTGTCCGTGTGGTTGCTAGTAGGGTGGGAGGGTGTTAGTGGTGTGTGCATTGGTGTTCTGGGGGTGTGGGGTTGGGTAGTGGTTAAAGCCCTGGTGATAATGGAGGATCTTGTCAGTAGCGTGGAAAGTTCATGGGGCAACGCGCATAGAATCTGCAGGGGAGAATTTATTTGCTATTCAATTCAGATCCAAGGGGGAAAAGATGCGAGTGATGACTACGGGCCGTGGGCTTTCGATCGTTCTCTTGTGGTCCTAAAGGAGCCGAGGTCCGCAGGGTCGGTATTGGATGTGGAGTTTAATGACTGTGTCTTTTGGGTCCAGATTCACAATGTACCTATGCGCTGGCAGACGAGAGAGATGGCTCAGTCTTTGGGTGGTTCTTTGAGGATGGATCGGCCGCTGAGGAGGGGGTTTCGGTTGCAAGATGAAGAGGGTAAAGAATTTCGGTGCCCGGTACTGTATGAGCGGCTGCCAGATTTTTGCTATGATTGTGGTTGTCTGGGGCACTCAAGAAGGGAGTGTGGGGTTGTTGGTGAAGGTGTGTCGGGTCAGCAGGGTGAGTAG

Protein sequence

MWLSVWLLVGWEGVSGVCIGVLGVWGWVVVKALVIMEDLVSSVESSWGNAHRICRGEFICYSIQIQGGKDASDDYGPWAFDRSLVVLKEPRSAGSVLDVEFNDCVFWVQIHNVPMRWQTREMAQSLGGSLRMDRPLRRGFRLQDEEGKEFRCPVLYERLPDFCYDCGCLGHSRRECGVVGEGVSGQQGE
Homology
BLAST of Lag0015313 vs. NCBI nr
Match: XP_022149484.1 (uncharacterized protein LOC111017902 [Momordica charantia])

HSP 1 Score: 124.4 bits (311), Expect = 1.0e-24
Identity = 63/172 (36.63%), Postives = 88/172 (51.16%), Query Frame = 0

Query: 35  IMEDLVSSVESSWGNAHRICRGEFICYSIQIQGGKDASD-----DYGPWAFDRSLVVLKE 94
           I  + + SV  S    H   R E +  +I +   K  S+       GPW F++SL+VL  
Sbjct: 49  ISAEAIRSVMKSVWRVHNSTRFEPLGMNIYVILFKSLSEKSRVLSSGPWTFNKSLLVLTS 108

Query: 95  PRSAGSVLDVEFNDCVFWVQIHNVPMRWQTREMAQSLGGSL------------------- 154
           P +    LD+ FN C FW+QIHN+P    + EMA  LG  L                   
Sbjct: 109 PTATNQPLDMNFNFCAFWIQIHNIPFECISTEMANILGAKLGDVEEIEGDGADGWAGPFI 168

Query: 155 ------RMDRPLRRGFRLQDEEGKEFRCPVLYERLPDFCYDCGCLGHSRREC 177
                  + +PLRRG +L++ +GK+  CP+ YE+LPDFCY+CG +GHS REC
Sbjct: 169 RVRVKIDVSKPLRRGIKLKNSDGKDIWCPLRYEKLPDFCYECGKIGHSGREC 220

BLAST of Lag0015313 vs. NCBI nr
Match: XP_022156711.1 (uncharacterized protein LOC111023555 [Momordica charantia])

HSP 1 Score: 110.9 bits (276), Expect = 1.2e-20
Identity = 50/125 (40.00%), Postives = 70/125 (56.00%), Query Frame = 0

Query: 76  GPWAFDRSLVVLKEPRSAGSVLDVEFNDCVFWVQIHNVPMRWQTREMAQSLGGSL----- 135
           GPW FD+SL++L    +A   LD++ + C FWVQIH +     T++MA+ LG  L     
Sbjct: 95  GPWTFDKSLLILVPTTAANKPLDIDISLCAFWVQIHYITFECMTKDMAKFLGARLGEVEE 154

Query: 136 --------------------RMDRPLRRGFRLQDEEGKEFRCPVLYERLPDFCYDCGCLG 176
                                + +PLRRG +++  +GK+  CP+ YERLPDFCY CGC+G
Sbjct: 155 VDGMSSYDWVRPFLWVRVKINVLKPLRRGLKVKTSDGKDIWCPLRYERLPDFCYGCGCVG 214

BLAST of Lag0015313 vs. NCBI nr
Match: XP_024033132.1 (uncharacterized protein LOC112095437 [Citrus clementina])

HSP 1 Score: 106.3 bits (264), Expect = 2.9e-19
Identity = 53/180 (29.44%), Postives = 87/180 (48.33%), Query Frame = 0

Query: 26  GWVVVKALVIMEDLVSSVESSWGNAH----RICRGEFICYSIQIQGGKDASDDYGPWAFD 85
           G +++   V  E L ++++ +W   H             +    +  K    + GPW FD
Sbjct: 43  GKILLARGVNREGLKTALDQAWRTVHDFKVESLGSNIFVFKFLSEADKKRVFNGGPWHFD 102

Query: 86  RSLVVLKEPRSAGSVLDVEFNDCVFWVQIHNVPMRWQTREMAQSLGG------------- 145
           R+L+VL+EP+  GS+    F+   FW++IHNVP+      + + LG              
Sbjct: 103 RALMVLQEPKGIGSIKKQSFSHVSFWIRIHNVPLMCMDTSIIRELGSRVGKVEDIGMDAQ 162

Query: 146 ------------SLRMDRPLRRGFRLQDEEGKEFRCPVLYERLPDFCYDCGCLGHSRREC 177
                       S+ + +PL++   L+ E+ ++   PV+YERLPDFC+ CGC+GH  REC
Sbjct: 163 GECFGEFVRIQVSVNITKPLKKLIVLKQEDDEDIPLPVVYERLPDFCFCCGCIGHQFREC 222

BLAST of Lag0015313 vs. NCBI nr
Match: TXG73177.1 (hypothetical protein EZV62_001756 [Acer yangbiense])

HSP 1 Score: 103.2 bits (256), Expect = 2.5e-18
Identity = 51/132 (38.64%), Postives = 69/132 (52.27%), Query Frame = 0

Query: 76  GPWAFDRSLVVLKEPRSAGSVLDVEFNDCVFWVQIHNVPMRWQTREMAQSLGGSLR---- 135
           GPW FDR++++ +EP  +G + D+EFN   FWVQIHN+PM   T E+   LG  +R    
Sbjct: 97  GPWRFDRAIIIFEEPTGSGEIADMEFNKTEFWVQIHNLPMICMTGEIGLFLGNMIREVRN 156

Query: 136 ---------------------MDRPLRRGFRLQD-EEGKEFRCPVLYERLPDFCYDCGCL 182
                                +D PL R  R+    +GK     + YER+PDFCY C  L
Sbjct: 157 VDLEAGKSGACRFIRVRVVIKVDEPLHRSLRVDILGDGKITTMLLRYERVPDFCYKCNRL 216

BLAST of Lag0015313 vs. NCBI nr
Match: XP_024036938.1 (uncharacterized protein LOC112096937 [Citrus clementina])

HSP 1 Score: 102.8 bits (255), Expect = 3.3e-18
Identity = 46/103 (44.66%), Postives = 60/103 (58.25%), Query Frame = 0

Query: 76  GPWAFDRSLVVLKEPRSAGSVLDVEFNDCVFWVQIHNVPMRWQTREMAQSLGGSL-RMDR 135
           GPW FDR+L VL EPR  G V    F    FWVQI NV +    ++  + LGG +  ++ 
Sbjct: 97  GPWHFDRALTVLAEPRGIGEVAKQSFTHTSFWVQIRNVSLACMDKDFLRDLGGKIGSVEE 156

Query: 136 PLRRGFRLQDEEGKEFRCPVLYERLPDFCYDCGCLGHSRRECG 178
           PL +   L+ E   +   PV+YE LPDFCY CG +GH  +ECG
Sbjct: 157 PLEKILFLKQEGETDIPIPVVYEHLPDFCYCCGIIGHQYKECG 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149484.11.0e-2436.63uncharacterized protein LOC111017902 [Momordica charantia][more]
XP_022156711.11.2e-2040.00uncharacterized protein LOC111023555 [Momordica charantia][more]
XP_024033132.12.9e-1929.44uncharacterized protein LOC112095437 [Citrus clementina][more]
TXG73177.12.5e-1838.64hypothetical protein EZV62_001756 [Acer yangbiense][more]
XP_024036938.13.3e-1844.66uncharacterized protein LOC112096937 [Citrus clementina][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025836Zinc knuckle CX2CX4HX4CPFAMPF14392zf-CCHC_4coord: 134..176
e-value: 6.8E-13
score: 48.1
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 133..177
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 51..127
NoneNo IPR availablePANTHERPTHR31286:SF84SUBFAMILY NOT NAMEDcoord: 51..127
NoneNo IPR availablePANTHERPTHR31286:SF84SUBFAMILY NOT NAMEDcoord: 133..177
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 163..176
score: 9.207683

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0015313.1Lag0015313.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding