Lag0015270 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015270
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	O-methyltransferase
Location	chr12: 9416896 .. 9418351 (+)
RNA-Seq Expression	Lag0015270
Synteny	Lag0015270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGATACAAAGATAGCAATTGAAGGCGAGGAGCTCTTAGAAGCACAAGCTCACATATGGAACCACATCTTCAACTTCATCAACTCCATGGCTTTGAACTGCGCCGTCCAACTCCACATTCCAGACGCCATCAGCCACCATGGCCACCCCATGCCCCTCTCTCAACTCATTTCAGCTCTCAAAATTGACCCCCAAAAGTCCCAACACATCCACCGCTTAATGCGTATATTGGCCCACTCTGGCTTCTTTCTAATACACGAAAATGTTGAAGAGCATCAAGAAGTAGCATATTCACTCACCAATTCATCTCTTCTTCTTCTTGAAGACAACCCTTTAACTCTATCTCCCTTTTTGCTTTCCATGCTCGATCAACTTCTCATGAAGTCATGGCAGTTTCTATCGGCCTGGCTTCGTAGCGACGGCGATCGAACCCCATTTCAGACAGCTCATGGAAAGCAATTTTGGGAGCATATGGAGAGTGAGTCAAGTGATAGGGAGGCTTTTAATGCAGCCATGGCTAGTGATGCGAGGTTGGTGATGAGTGTTTTGTTGGGGAAATATAAGAGTGTGTTTGAGGGAGTTGAGTTGTTGGTTGATGTGGGAGGTGGCACTGGAACCATGGCCAGAGCCATTGCTGAGGCCTTTCCACAAATGCAATGTGTTGTGCTTGACCTTCCCCAAGTTGTGGCTGGCTTGGAAGGAAATGATCACAACTTGACATTTCTTCAAGGAGATATGTTTCAAACCATTCCTCATGCTGATGCACTCTTATTGAAGGTGAGAAGTTAAATTTTAATTTTGATTTAGTTTGTTATAAGTTCATTTCTTGAATGTTAATTGAGTTCTAACTTTAATTTGTCATTTTAACACTTATAACTTTAAGCACCCGAACATGGCTAAAGTTAGTTGTAAAATTGACAATGTTAACAATGACGTATTAGATATAAAATTGATAGTTGAATTGAAATGCTTTAATTAAAGTAATGAGACTAGATAGACATAATCCTAAAAGTTAAGGAACCTATTAAATGCACAAAACAATTAGTTCTAAAATCAAATTTCTAATTTAAGATTTAGTTTGTTATTGTGTTGTTTTAGTTGTATATATACATACTCATAAGAGTTTGATGTGAAACGTGATATAACACAGTGGATATTACACGATTGGAGTGACGAAGAATGTGTGAAGATACTGAAAAAATGTAAAGAGGCAATCATAAGCAATGGGAAGAAAGGAAAAGTGATGGTACTTGATATGGTGGTAGACCATGAAAAAAAGAATCATATGTTGACTGAAACTCAACTATTCTGGGACGTGTTGATGATGGCTAATGTAGGTGGAAAAGAAAGAGATGAGAAAGAGTGGGCCCAATTGTTCTGTGAAGCTGGATTTGGTGGCTACAAGATCTTTCCAATTTTGGGTTTAAGGTCGCTTATAGAGATTTATCCATAG

mRNA sequence

ATGAAGGATACAAAGATAGCAATTGAAGGCGAGGAGCTCTTAGAAGCACAAGCTCACATATGGAACCACATCTTCAACTTCATCAACTCCATGGCTTTGAACTGCGCCGTCCAACTCCACATTCCAGACGCCATCAGCCACCATGGCCACCCCATGCCCCTCTCTCAACTCATTTCAGCTCTCAAAATTGACCCCCAAAAGTCCCAACACATCCACCGCTTAATGCGTATATTGGCCCACTCTGGCTTCTTTCTAATACACGAAAATGTTGAAGAGCATCAAGAAGTAGCATATTCACTCACCAATTCATCTCTTCTTCTTCTTGAAGACAACCCTTTAACTCTATCTCCCTTTTTGCTTTCCATGCTCGATCAACTTCTCATGAAGTCATGGCAGTTTCTATCGGCCTGGCTTCGTAGCGACGGCGATCGAACCCCATTTCAGACAGCTCATGGAAAGCAATTTTGGGAGCATATGGAGAGTGAGTCAAGTGATAGGGAGGCTTTTAATGCAGCCATGGCTAGTGATGCGAGGTTGGTGATGAGTGTTTTGTTGGGGAAATATAAGAGTGTGTTTGAGGGAGTTGAGTTGTTGGTTGATGTGGGAGGTGGCACTGGAACCATGGCCAGAGCCATTGCTGAGGCCTTTCCACAAATGCAATGTGTTGTGCTTGACCTTCCCCAAGTTGTGGCTGGCTTGGAAGGAAATGATCACAACTTGACATTTCTTCAAGGAGATATGTTTCAAACCATTCCTCATGCTGATGCACTCTTATTGAAGTGGATATTACACGATTGGAGTGACGAAGAATGTGTGAAGATACTGAAAAAATGTAAAGAGGCAATCATAAGCAATGGGAAGAAAGGAAAAGTGATGGTACTTGATATGGTGGTAGACCATGAAAAAAAGAATCATATGTTGACTGAAACTCAACTATTCTGGGACGTGTTGATGATGGCTAATGTAGGTGGAAAAGAAAGAGATGAGAAAGAGTGGGCCCAATTGTTCTGTGAAGCTGGATTTGGTGGCTACAAGATCTTTCCAATTTTGGGTTTAAGGTCGCTTATAGAGATTTATCCATAG

Coding sequence (CDS)

ATGAAGGATACAAAGATAGCAATTGAAGGCGAGGAGCTCTTAGAAGCACAAGCTCACATATGGAACCACATCTTCAACTTCATCAACTCCATGGCTTTGAACTGCGCCGTCCAACTCCACATTCCAGACGCCATCAGCCACCATGGCCACCCCATGCCCCTCTCTCAACTCATTTCAGCTCTCAAAATTGACCCCCAAAAGTCCCAACACATCCACCGCTTAATGCGTATATTGGCCCACTCTGGCTTCTTTCTAATACACGAAAATGTTGAAGAGCATCAAGAAGTAGCATATTCACTCACCAATTCATCTCTTCTTCTTCTTGAAGACAACCCTTTAACTCTATCTCCCTTTTTGCTTTCCATGCTCGATCAACTTCTCATGAAGTCATGGCAGTTTCTATCGGCCTGGCTTCGTAGCGACGGCGATCGAACCCCATTTCAGACAGCTCATGGAAAGCAATTTTGGGAGCATATGGAGAGTGAGTCAAGTGATAGGGAGGCTTTTAATGCAGCCATGGCTAGTGATGCGAGGTTGGTGATGAGTGTTTTGTTGGGGAAATATAAGAGTGTGTTTGAGGGAGTTGAGTTGTTGGTTGATGTGGGAGGTGGCACTGGAACCATGGCCAGAGCCATTGCTGAGGCCTTTCCACAAATGCAATGTGTTGTGCTTGACCTTCCCCAAGTTGTGGCTGGCTTGGAAGGAAATGATCACAACTTGACATTTCTTCAAGGAGATATGTTTCAAACCATTCCTCATGCTGATGCACTCTTATTGAAGTGGATATTACACGATTGGAGTGACGAAGAATGTGTGAAGATACTGAAAAAATGTAAAGAGGCAATCATAAGCAATGGGAAGAAAGGAAAAGTGATGGTACTTGATATGGTGGTAGACCATGAAAAAAAGAATCATATGTTGACTGAAACTCAACTATTCTGGGACGTGTTGATGATGGCTAATGTAGGTGGAAAAGAAAGAGATGAGAAAGAGTGGGCCCAATTGTTCTGTGAAGCTGGATTTGGTGGCTACAAGATCTTTCCAATTTTGGGTTTAAGGTCGCTTATAGAGATTTATCCATAG

Protein sequence

MKDTKIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKIDPQKSQHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPFLLSMLDQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVVDHEKKNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP

Homology

[Close]

BLAST of Lag0015270 vs. NCBI nr
Match: XP_023548392.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.0 bits (1473), Expect = 3.6e-159
Identity = 285/359 (79.39%), Postives = 317/359 (88.30%), Query Frame = 0


Query: 5   KIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKID 64
           ++A++GEELL AQAHIWNHIFNFINSMAL CAVQL IPDAISHHG PM L QL+S+L++D
Sbjct: 11  EMAMKGEELLNAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPMTLPQLVSSLQVD 70

Query: 65  PQKSQHIHRLMRILAHSGFFLIHENVEE--HQEVAYSLTNSSLLLLEDNPLTLSPFLLSM 124
           PQKSQHIHRLMRILA++GFFLIHE  +E   QEVAYSLTNSSLLLLE NPLTLSPFLLSM
Sbjct: 71  PQKSQHIHRLMRILAYAGFFLIHEPSKEGDDQEVAYSLTNSSLLLLEHNPLTLSPFLLSM 130

Query: 125 LDQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMS 184
           LD +L+  WQF+S WLRSD DRTPF+TAHGKQFWEHM  +  D   FNAAMASDARLV S
Sbjct: 131 LDPVLVDPWQFISTWLRSDRDRTPFETAHGKQFWEHMGGKPKDGMVFNAAMASDARLVTS 190

Query: 185 VLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTF 244
           VLLGKYKSVFEGVELLVDVGGGTGT+ +AIAEAFPQMQCVV DLPQVVA L+G+ HNL F
Sbjct: 191 VLLGKYKSVFEGVELLVDVGGGTGTVGQAIAEAFPQMQCVVFDLPQVVADLKGS-HNLMF 250

Query: 245 LQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV-DHE 304
           ++GDMFQ IP A A+LLKWILHDWSDEECVKILK+CKEAI SNGKKGKVM++D+VV D +
Sbjct: 251 VEGDMFQQIPPAGAILLKWILHDWSDEECVKILKRCKEAITSNGKKGKVMIIDLVVRDED 310

Query: 305 KKNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
            KN MLTETQLFWDVLMM  VGG+ER+EKEWA+LF +AGFGGY IFPILGLRSLIEIYP
Sbjct: 311 IKNDMLTETQLFWDVLMMVTVGGRERNEKEWAELFYKAGFGGYNIFPILGLRSLIEIYP 368

[Close]

BLAST of Lag0015270 vs. NCBI nr
Match: XP_022991753.1 (probable O-methyltransferase 3 [Cucurbita maxima])

HSP 1 Score: 565.8 bits (1457), Expect = 2.6e-157
Identity = 281/362 (77.62%), Postives = 318/362 (87.85%), Query Frame = 0


Query: 2   KDTKIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISAL 61
           K  ++A++GEELL+AQAHIWNHIFNFINSMAL CAVQL IPDAISHHG PM L QL+S+L
Sbjct: 42  KGIEMAMKGEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPMTLPQLVSSL 101

Query: 62  KIDPQKSQHIHRLMRILAHSGFFLIHENVEE--HQEVAYSLTNSSLLLLEDNPLTLSPFL 121
           ++DPQKSQHIHRLMRILA++GFFLIHE  +E   QEVAYSLTNSSLLLLE NP TLSPFL
Sbjct: 102 QVDPQKSQHIHRLMRILAYAGFFLIHEPSKEGDDQEVAYSLTNSSLLLLEHNPFTLSPFL 161

Query: 122 LSMLDQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARL 181
           LSMLD +L+  W+F+S WLR+D DRTPF+TAHGKQFWEHM  +  D   FNAAMASD+RL
Sbjct: 162 LSMLDPVLVDPWKFISTWLRNDCDRTPFETAHGKQFWEHMGGKPKDGMVFNAAMASDSRL 221

Query: 182 VMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHN 241
           V SVLLGK+KSVFEGVELLVDVGGGTGT+A+AIAEAFPQMQCVV DLPQVV  L+G+ HN
Sbjct: 222 VTSVLLGKHKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQVVGDLKGS-HN 281

Query: 242 LTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV- 301
           LTF +GDMFQ IP ADALLLKWILHDWSDEECV+ILK+CKEAI SNGKKGKVM++D+VV 
Sbjct: 282 LTFAEGDMFQQIPPADALLLKWILHDWSDEECVEILKRCKEAITSNGKKGKVMIIDLVVR 341

Query: 302 DHEKKNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEI 361
           D + KN MLTET+LFWD LMM  VGG+ER+EKEWA+LF +AGFGGY IFPILGLRSLIEI
Sbjct: 342 DEDNKNDMLTETRLFWDELMMMTVGGRERNEKEWAELFYKAGFGGYTIFPILGLRSLIEI 401

[Close]

BLAST of Lag0015270 vs. NCBI nr
Match: KAG6575317.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 562.0 bits (1447), Expect = 3.7e-156
Identity = 280/358 (78.21%), Postives = 313/358 (87.43%), Query Frame = 0


Query: 6   IAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKIDP 65
           +A++GEELL+AQAHIWNHIFNFINSMAL CAVQL IPDAISHHG PM L QL+S+L++DP
Sbjct: 1   MAMKGEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPMTLPQLVSSLQVDP 60

Query: 66  QKSQHIHRLMRILAHSGFFLIHENVE--EHQEVAYSLTNSSLLLLEDNPLTLSPFLLSML 125
           QKSQHIHRLMRILA++GFFL+HE  +  + QEVAYSLTNSSLLLLE N LTLSPFLLSML
Sbjct: 61  QKSQHIHRLMRILAYAGFFLVHETSKQGDDQEVAYSLTNSSLLLLEHNTLTLSPFLLSML 120

Query: 126 DQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSV 185
           D +L+  WQF+S WLR+D DRTPF+TAHGKQFWEHM  E      FNAAMASDARLV SV
Sbjct: 121 DPVLVDPWQFISTWLRNDRDRTPFETAHGKQFWEHMGGEPKAGMVFNAAMASDARLVTSV 180

Query: 186 LLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFL 245
           LLG YKSVFEGVELLVDVGGGTGT+A+AIAEAFPQMQCVV DLPQVV  L+G+  NL F+
Sbjct: 181 LLGTYKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQVVGDLKGS-QNLIFV 240

Query: 246 QGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV-DHEK 305
           +GDMFQ IP ADALL+KWILHDWSDEEC KILKKCKEAI SNGKKGKVM++D+VV D + 
Sbjct: 241 EGDMFQQIPPADALLMKWILHDWSDEECGKILKKCKEAITSNGKKGKVMIIDLVVSDEDN 300

Query: 306 KNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
           KN MLTETQLFWDVLMM  VGG+ER+EKEWA+LF +AGFGGY IFPILGLRSLIEIYP
Sbjct: 301 KNDMLTETQLFWDVLMMVTVGGRERNEKEWAELFYKAGFGGYNIFPILGLRSLIEIYP 357

[Close]

BLAST of Lag0015270 vs. NCBI nr
Match: XP_022929871.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 561.2 bits (1445), Expect = 6.4e-156
Identity = 280/358 (78.21%), Postives = 313/358 (87.43%), Query Frame = 0


Query: 6   IAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKIDP 65
           +A++GEELL+AQAHIWNHIFNFINSMAL CAVQL IPDAISHHG P+ LSQL+S+L++DP
Sbjct: 1   MAMKGEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPITLSQLVSSLQVDP 60

Query: 66  QKSQHIHRLMRILAHSGFFLIHENVE--EHQEVAYSLTNSSLLLLEDNPLTLSPFLLSML 125
           QKSQHIHRLMRILA++GFFLIHE  +  + QEVAYSLTNSSLLLLE N L LSPFLLSML
Sbjct: 61  QKSQHIHRLMRILAYAGFFLIHETSKQGDDQEVAYSLTNSSLLLLEHNTLALSPFLLSML 120

Query: 126 DQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSV 185
           D +L+  WQF+S WLR+D DRTPF+TAHGKQFWEHM  E  D   F AAMASDARLV SV
Sbjct: 121 DPVLVDPWQFISTWLRNDRDRTPFETAHGKQFWEHMGGELKDGMVFIAAMASDARLVTSV 180

Query: 186 LLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFL 245
           LLG YKSVFEGVELLVDVGGGTGT+A+AIAEAFPQMQCVV DLPQVV  L+G+  NL F+
Sbjct: 181 LLGTYKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQVVGDLKGS-QNLIFV 240

Query: 246 QGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV-DHEK 305
           +GDMFQ IP ADALL+KWILHDWSDEEC KILKKCKEAI SNGKKGKVM++D+VV D + 
Sbjct: 241 EGDMFQQIPPADALLMKWILHDWSDEECAKILKKCKEAITSNGKKGKVMIIDLVVRDEDN 300

Query: 306 KNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
           KN MLTETQLFWDVLMM  VGG+ER+EKEWA+LF +AGFGGY IFPILGLRSLIEIYP
Sbjct: 301 KNDMLTETQLFWDVLMMVTVGGRERNEKEWAELFYKAGFGGYNIFPILGLRSLIEIYP 357

[Close]

BLAST of Lag0015270 vs. NCBI nr
Match: XP_011659150.1 (trans-resveratrol di-O-methyltransferase [Cucumis sativus] >KGN44537.1 hypothetical protein Csa_016151 [Cucumis sativus])

HSP 1 Score: 537.7 bits (1384), Expect = 7.5e-149
Identity = 271/376 (72.07%), Postives = 316/376 (84.04%), Query Frame = 0


Query: 2   KDTKIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISAL 61
           ++T++ ++GEE+L+AQAHIWNHIF+FINSMAL CAV+L IPDAI+ HG P+ LSQLISAL
Sbjct: 4   RNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISAL 63

Query: 62  KIDPQKSQHIHRLMRILAHSGFFLIHENV---------EEHQEVAYSLTNSSLLLLEDNP 121
           +I PQKSQH+HR+MRILAHSGFFLIH  +         E+  +VAYSLTN SLLLL+ NP
Sbjct: 64  QIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNP 123

Query: 122 LTLSPFLLSMLDQLLMKSWQFLSAWLRSDGD-RTPFQTAHGKQFWEHMESESSDREAFNA 181
           LTLSPF L MLD++L++ W F+SAWLRSD D RTPF+TAHGK+FWEHM SE+ D E FNA
Sbjct: 124 LTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNA 183

Query: 182 AMASDARLVMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVA 241
           AM SDA+LV+SVLL KYKSVFEGVE  VDVGGGTG++A+AIAEAFPQM+CVV DLPQVVA
Sbjct: 184 AMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVA 243

Query: 242 GLEGNDHNLTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKV 301
           GLEGN HNL FLQGDMFQ IP ADALLLKWILHDW+D+ECVKILKKCKEAI SNGK GKV
Sbjct: 244 GLEGN-HNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKV 303

Query: 302 MVLDMVVDHE------KKNHML-TETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGY 361
           +++DMVV ++      K N ML T+ +LFWD+LMM NVGGKERDEKEWA+LF  AGFG Y
Sbjct: 304 IIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAY 363

[Close]

BLAST of Lag0015270 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.0e-111
Identity = 214/355 (60.28%), Postives = 269/355 (75.77%), Query Frame = 0


Query: 12  ELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKIDPQKSQHI 71
           E+L AQAHIWN IFNFINSM+L CA+QL IPD I++HG PM +SQL  AL I+ +KS  +
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPINRKKSPCV 84

Query: 72  HRLMRILAHSGFFLIHE-NVEEH---QEVAYSLTNSSLLLLEDNPLTLSPFLLSMLDQLL 131
           +RLMRIL HSGFF + +  V E    +E  Y +T++S LLL+DNP++++PFLL+MLD ++
Sbjct: 85  YRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDPVM 144

Query: 132 MKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVLLGK 191
            K W FLS W + +GD TPF TA+G  FW++   E      FN AMASDARLV SV++ K
Sbjct: 145 TKPWDFLSNWFQ-NGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVIEK 204

Query: 192 YKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQGDM 251
            K VFEGVE LVDVGGGTGT+A +IA AFP +QC V DLP VVA L+G  +NL F+ GDM
Sbjct: 205 CKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGG-NNLNFVGGDM 264

Query: 252 FQTIPHADALLLKWILHDWSDEECVKILKKCKEAII-SNGKKGKVMVLDMVVDHEK-KNH 311
           F  +P  + +LLKWILHDW+DEE VKILKKCKEAI  SN K GKV+++DM V++EK ++ 
Sbjct: 265 FVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENEKDEDD 324

Query: 312 MLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
              ETQLF+D+LMMA V G+ER+EKEWA+LF +AGF  YKI PILGLRSLIE+YP
Sbjct: 325 ESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

[Close]

BLAST of Lag0015270 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 397.5 bits (1020), Expect = 1.6e-109
Identity = 197/349 (56.45%), Postives = 256/349 (73.35%), Query Frame = 0


Query: 12  ELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKIDPQKSQHI 71
           ELL AQAH+WNHIFNFI SM+L CA+QL IPD I +HG PM L +L++ L + P++SQ +
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKRSQCV 70

Query: 72  HRLMRILAHSGFFLIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPFLLSMLDQLLMKSW 131
           +RLMRIL HSGF       +  +E  Y LT++S LLL D+ L++ P +L+MLD +L K W
Sbjct: 71  YRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPILTKPW 130

Query: 132 QFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVLLGKYKSV 191
            +LSAW ++D D TPF TA+ + FW++   E     +FN AMASDARL+ SVLL + + V
Sbjct: 131 HYLSAWFQND-DPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKEGQGV 190

Query: 192 FEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQGDMFQTI 251
           F G+  LVDVGGGTG +A+AIA AFP + C VLDL  VVAGL+G+  NL +  GDMF+ I
Sbjct: 191 FAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGS-KNLNYFAGDMFEAI 250

Query: 252 PHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVVDHEKKNHMLTETQ 311
           P ADA+LLKWILHDWS+EECVKILK+C+EAI S    GKV+++DM++   + ++  TETQ
Sbjct: 251 PPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYKSTETQ 310

Query: 312 LFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
           LF+D+ MM    G+ERDE EW +LF +AGF  YKI PILGLRSLIE+YP
Sbjct: 311 LFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

[Close]

BLAST of Lag0015270 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 3.2e-102
Identity = 191/358 (53.35%), Postives = 255/358 (71.23%), Query Frame = 0


Query: 11  EELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKIDPQKSQH 70
           +ELLEAQAH+WNHI+++INSM+L CA+QL IPDAI  HG+P+ LSQL  AL I+  KS  
Sbjct: 11  QELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINKAKSHG 70

Query: 71  IHRLMRILAHSGFF-------LIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPFLLSML 130
           + RLMRIL HSGFF        +    EE +E AYSLT +S LLL   PL+++PF L+M 
Sbjct: 71  LFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFALAMS 130

Query: 131 DQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSV 190
           D +  ++W  LS W R+D     F T +G  F E+  ++      FN AMA DA  V S+
Sbjct: 131 DPVYTETWHHLSEWFRNDA-VAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFVNSI 190

Query: 191 LLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFL 250
           L  + + +F+G+E +VDVGGGTG  A+ IA AFP M+C VLDLP VV GL+G++ NL+F+
Sbjct: 191 LTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSE-NLSFV 250

Query: 251 QGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAII-SNGKKGKVMVLDMVVDHEK 310
            GDMF  IPHADA+ +K+ILHDW+DEECVKILKKCKEAI  SN    K++++++V++ EK
Sbjct: 251 SGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKIILVEIVMEDEK 310

Query: 311 KNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
           + H  TET+LF+D+ M+A + GKER EKEW +LF +AGF  YKI  +LGLRS+IE++P
Sbjct: 311 ETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGLRSVIEVFP 366

[Close]

BLAST of Lag0015270 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 5.1e-100
Identity = 186/353 (52.69%), Postives = 248/353 (70.25%), Query Frame = 0


Query: 10  GEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAI-SHHGHPMPLSQLISALKIDPQKS 69
           GEELL AQAHI+NHIFNFI+SMAL CAV+L+IP+ + +H    + LS+L+ AL+I   KS
Sbjct: 21  GEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSELVQALQIPQAKS 80

Query: 70  QHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPFLLSMLDQLLM 129
             ++RL+RIL HSGFF I +   E  E  Y  T SS LLL+++P+++SP LL +++  ++
Sbjct: 81  ACLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLKNHPMSMSPCLLGLVNPTMV 140

Query: 130 KSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVLLGKY 189
               F S W +   D TPF+  HG   W++        E FN AM  + RL MSV+L + 
Sbjct: 141 APMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEIFNEAMGCETRLAMSVVLKEC 200

Query: 190 KSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQGDMF 249
           K   EG+  LVDVGGGTG + RAIAEAFP ++C VLDLPQVV  L+G+ +NL F+ GDMF
Sbjct: 201 KGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGS-NNLEFVSGDMF 260

Query: 250 QTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV-DHEKKNHML 309
           Q IP AD + LKWILHDW+DEEC+KILK+CKEAI S  + GK++++DMVV DH K ++  
Sbjct: 261 QFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVNDHNKGSYES 320

Query: 310 TETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
           TETQLF+D+ +MA + G ER E EW +LF  AGF  Y I P+LGL+S+IE++P
Sbjct: 321 TETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLGLKSIIEVFP 372

[Close]

BLAST of Lag0015270 vs. ExPASy Swiss-Prot
Match: B0EXJ8 (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 5.0e-95
Identity = 182/357 (50.98%), Postives = 247/357 (69.19%), Query Frame = 0


Query: 8   IEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISAL--KIDP 67
           ++ EE   AQA IW+   +FI S +L CAV+L IPD I +HG P+ LS+L +AL   + P
Sbjct: 3   VQSEEFRGAQAQIWSQSCSFITSASLKCAVKLGIPDTIDNHGKPITLSELTNALVPPVHP 62

Query: 68  QKSQHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPFLLSMLDQ 127
            K+  I+RLMR+LA +G F   E ++   E  YSLT SS +LL+  PL L   +L+M D 
Sbjct: 63  SKAPFIYRLMRVLAKNG-FCSEEQLDGETEPLYSLTPSSRILLKKEPLNLRGIVLTMADP 122

Query: 128 LLMKSWQFLSAWLRSDGD-RTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVL 187
           + +K+W+ LS W +++ D  T F+TAHGK FW +        E FN AMASD++L+  +L
Sbjct: 123 VQLKAWESLSDWYQNEDDSSTAFETAHGKNFWGYSSEHMEHAEFFNEAMASDSQLISKLL 182

Query: 188 LGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQ 247
           +G+YK +FEG+  LVD+GGGTGT+A+AIA+ FPQ++C V DLP VVA LE  + N+ F+ 
Sbjct: 183 IGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKE-NVEFVA 242

Query: 248 GDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVVDHEKKN 307
           GDMF+ IP A+A+ LKWILHDW+DE+CVKILK CK+AI + G  GKV+++DMV+  +KK+
Sbjct: 243 GDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKG--GKVIIIDMVMYSDKKD 302

Query: 308 HMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGL-RSLIEIYP 361
             L +TQ   D+ M+ N   KER EKEWA LF EAGF  YKI+P L   RSLIE+YP
Sbjct: 303 DHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIEVYP 355

[Close]

BLAST of Lag0015270 vs. ExPASy TrEMBL
Match: A0A6J1JX64 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111488285 PE=3 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.2e-157
Identity = 281/362 (77.62%), Postives = 318/362 (87.85%), Query Frame = 0


Query: 2   KDTKIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISAL 61
           K  ++A++GEELL+AQAHIWNHIFNFINSMAL CAVQL IPDAISHHG PM L QL+S+L
Sbjct: 42  KGIEMAMKGEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPMTLPQLVSSL 101

Query: 62  KIDPQKSQHIHRLMRILAHSGFFLIHENVEE--HQEVAYSLTNSSLLLLEDNPLTLSPFL 121
           ++DPQKSQHIHRLMRILA++GFFLIHE  +E   QEVAYSLTNSSLLLLE NP TLSPFL
Sbjct: 102 QVDPQKSQHIHRLMRILAYAGFFLIHEPSKEGDDQEVAYSLTNSSLLLLEHNPFTLSPFL 161

Query: 122 LSMLDQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARL 181
           LSMLD +L+  W+F+S WLR+D DRTPF+TAHGKQFWEHM  +  D   FNAAMASD+RL
Sbjct: 162 LSMLDPVLVDPWKFISTWLRNDCDRTPFETAHGKQFWEHMGGKPKDGMVFNAAMASDSRL 221

Query: 182 VMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHN 241
           V SVLLGK+KSVFEGVELLVDVGGGTGT+A+AIAEAFPQMQCVV DLPQVV  L+G+ HN
Sbjct: 222 VTSVLLGKHKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQVVGDLKGS-HN 281

Query: 242 LTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV- 301
           LTF +GDMFQ IP ADALLLKWILHDWSDEECV+ILK+CKEAI SNGKKGKVM++D+VV 
Sbjct: 282 LTFAEGDMFQQIPPADALLLKWILHDWSDEECVEILKRCKEAITSNGKKGKVMIIDLVVR 341

Query: 302 DHEKKNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEI 361
           D + KN MLTET+LFWD LMM  VGG+ER+EKEWA+LF +AGFGGY IFPILGLRSLIEI
Sbjct: 342 DEDNKNDMLTETRLFWDELMMMTVGGRERNEKEWAELFYKAGFGGYTIFPILGLRSLIEI 401

[Close]

BLAST of Lag0015270 vs. ExPASy TrEMBL
Match: A0A6J1EPC7 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111436348 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 3.1e-156
Identity = 280/358 (78.21%), Postives = 313/358 (87.43%), Query Frame = 0


Query: 6   IAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKIDP 65
           +A++GEELL+AQAHIWNHIFNFINSMAL CAVQL IPDAISHHG P+ LSQL+S+L++DP
Sbjct: 1   MAMKGEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPITLSQLVSSLQVDP 60

Query: 66  QKSQHIHRLMRILAHSGFFLIHENVE--EHQEVAYSLTNSSLLLLEDNPLTLSPFLLSML 125
           QKSQHIHRLMRILA++GFFLIHE  +  + QEVAYSLTNSSLLLLE N L LSPFLLSML
Sbjct: 61  QKSQHIHRLMRILAYAGFFLIHETSKQGDDQEVAYSLTNSSLLLLEHNTLALSPFLLSML 120

Query: 126 DQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSV 185
           D +L+  WQF+S WLR+D DRTPF+TAHGKQFWEHM  E  D   F AAMASDARLV SV
Sbjct: 121 DPVLVDPWQFISTWLRNDRDRTPFETAHGKQFWEHMGGELKDGMVFIAAMASDARLVTSV 180

Query: 186 LLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFL 245
           LLG YKSVFEGVELLVDVGGGTGT+A+AIAEAFPQMQCVV DLPQVV  L+G+  NL F+
Sbjct: 181 LLGTYKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQVVGDLKGS-QNLIFV 240

Query: 246 QGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV-DHEK 305
           +GDMFQ IP ADALL+KWILHDWSDEEC KILKKCKEAI SNGKKGKVM++D+VV D + 
Sbjct: 241 EGDMFQQIPPADALLMKWILHDWSDEECAKILKKCKEAITSNGKKGKVMIIDLVVRDEDN 300

Query: 306 KNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
           KN MLTETQLFWDVLMM  VGG+ER+EKEWA+LF +AGFGGY IFPILGLRSLIEIYP
Sbjct: 301 KNDMLTETQLFWDVLMMVTVGGRERNEKEWAELFYKAGFGGYNIFPILGLRSLIEIYP 357

[Close]

BLAST of Lag0015270 vs. ExPASy TrEMBL
Match: A0A0A0K881 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G328250 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.6e-149
Identity = 271/376 (72.07%), Postives = 316/376 (84.04%), Query Frame = 0


Query: 2   KDTKIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISAL 61
           ++T++ ++GEE+L+AQAHIWNHIF+FINSMAL CAV+L IPDAI+ HG P+ LSQLISAL
Sbjct: 4   RNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISAL 63

Query: 62  KIDPQKSQHIHRLMRILAHSGFFLIHENV---------EEHQEVAYSLTNSSLLLLEDNP 121
           +I PQKSQH+HR+MRILAHSGFFLIH  +         E+  +VAYSLTN SLLLL+ NP
Sbjct: 64  QIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNP 123

Query: 122 LTLSPFLLSMLDQLLMKSWQFLSAWLRSDGD-RTPFQTAHGKQFWEHMESESSDREAFNA 181
           LTLSPF L MLD++L++ W F+SAWLRSD D RTPF+TAHGK+FWEHM SE+ D E FNA
Sbjct: 124 LTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNA 183

Query: 182 AMASDARLVMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVA 241
           AM SDA+LV+SVLL KYKSVFEGVE  VDVGGGTG++A+AIAEAFPQM+CVV DLPQVVA
Sbjct: 184 AMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVA 243

Query: 242 GLEGNDHNLTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKV 301
           GLEGN HNL FLQGDMFQ IP ADALLLKWILHDW+D+ECVKILKKCKEAI SNGK GKV
Sbjct: 244 GLEGN-HNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKV 303

Query: 302 MVLDMVVDHE------KKNHML-TETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGY 361
           +++DMVV ++      K N ML T+ +LFWD+LMM NVGGKERDEKEWA+LF  AGFG Y
Sbjct: 304 IIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAY 363

[Close]

BLAST of Lag0015270 vs. ExPASy TrEMBL
Match: A0A6J1DVA5 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111023429 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 6.9e-140
Identity = 255/362 (70.44%), Postives = 298/362 (82.32%), Query Frame = 0


Query: 8   IEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGH-PMPLSQLISALKIDPQ 67
           + G E+LEA+ HIWNHIF+FINSMAL CAVQL IPD I+HHG  PM LS L+SALK+ P+
Sbjct: 4   VAGNEVLEAETHIWNHIFSFINSMALKCAVQLGIPDTIAHHGRGPMSLSHLVSALKLHPE 63

Query: 68  KSQHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPFLLSMLDQL 127
            SQ++HRLMRILAHSGFF  H+N ++ QE AYSLT+SS LLL+ NP +LSP LLSMLD  
Sbjct: 64  NSQYLHRLMRILAHSGFFTAHKN-DKEQEEAYSLTSSSRLLLKCNPSSLSPLLLSMLDPF 123

Query: 128 LMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVLLG 187
           L++ WQ LSAW  S  DRT F+ AHGKQFWEHM S+  D + FNAAMASDARLV SVLLG
Sbjct: 124 LLQPWQLLSAWFHSH-DRTAFKMAHGKQFWEHMASKPRDGDVFNAAMASDARLVTSVLLG 183

Query: 188 KYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQGD 247
           +YK++F+GV+ LVDVGGGTGT+AR IA+ FPQ+QCVVLDLP VVA LEG+ HNLT+L+GD
Sbjct: 184 QYKNIFDGVKSLVDVGGGTGTVARVIADVFPQIQCVVLDLPHVVADLEGS-HNLTYLEGD 243

Query: 248 MFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVVDHEK---- 307
           MFQ+IP AD LLLKWILHDWSD EC +ILKKCKEAI SNGKKG+V+++DMVV ++K    
Sbjct: 244 MFQSIPSADVLLLKWILHDWSDGECAEILKKCKEAITSNGKKGRVILIDMVVGNKKVDGD 303

Query: 308 ----KNHMLTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEI 361
                + M  ETQLFWD+LMM NVGGKERDEKEW QLF +AGFGGYKIFPILGLRSLIEI
Sbjct: 304 GDRDGDDMFIETQLFWDMLMMVNVGGKERDEKEWVQLFDKAGFGGYKIFPILGLRSLIEI 362

[Close]

BLAST of Lag0015270 vs. ExPASy TrEMBL
Match: A0A6J1DTU5 (trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111023435 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.9e-137
Identity = 253/354 (71.47%), Postives = 296/354 (83.62%), Query Frame = 0


Query: 8   IEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHG-HPMPLSQLISALKIDPQ 67
           + GEE+LEA+A IWNHIF+FINSMAL C VQL IPDAI+ H   PMPLS LISAL++ PQ
Sbjct: 4   LAGEEVLEAEARIWNHIFSFINSMALKCVVQLGIPDAIARHDCSPMPLSHLISALQLHPQ 63

Query: 68  KSQHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPFLLSMLDQL 127
            SQ+IHRLM ILAHSGFF+ H+N  E  E AYSLTNSS LLL+ NP +LSPFLLSMLD L
Sbjct: 64  NSQYIHRLMCILAHSGFFITHKNGSE--EEAYSLTNSSRLLLKHNPSSLSPFLLSMLDPL 123

Query: 128 LMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVLLG 187
           L++ WQ LSAW  S  +RTPF+ AHG+QFWEHM ++  D + FNAAMASDARLV ++LLG
Sbjct: 124 LLQPWQLLSAWFHS-LNRTPFEMAHGRQFWEHMGNKQQDGDVFNAAMASDARLVSNILLG 183

Query: 188 KYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQGD 247
           +YK++F+GV+ LVDVGGGTGTMA+AIA+AFPQ QCVVLDL  VVA LEG+ HNLT+L GD
Sbjct: 184 QYKNMFDGVKSLVDVGGGTGTMAKAIADAFPQTQCVVLDLFHVVADLEGS-HNLTYLPGD 243

Query: 248 MFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVVDHEKKNHM 307
           MFQ+IP AD LLLKWILHDWSD ECV+ILKKCKEAI SNGK GKV+++DMV+ ++K +  
Sbjct: 244 MFQSIPPADVLLLKWILHDWSDGECVEILKKCKEAITSNGKTGKVILIDMVLGNKKGDDT 303

Query: 308 LTETQLFWDVLMMANVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRSLIEIYP 361
             ETQLFWD+LMMANV GKERDEKEWAQLF +A F GYKIFP+LGLRSLIEIYP
Sbjct: 304 FIETQLFWDMLMMANVSGKERDEKEWAQLFDKARFAGYKIFPVLGLRSLIEIYP 353

[Close]

BLAST of Lag0015270 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 212.6 bits (540), Expect = 5.2e-55
Identity = 132/376 (35.11%), Postives = 202/376 (53.72%), Query Frame = 0


Query: 1   MKDTKIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHH--GHPMPLSQLI 60
           M+D K  +  EE  +A   IW ++F F +  A  CA+ L IP+AI +H    P+ L++L 
Sbjct: 15  MEDNKRKVLDEE-AKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELS 74

Query: 61  SALKIDPQKSQHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTNS----SLLLLEDNPLT 120
           SA+   P    H+ R+MR L H G F   + +     +A    N+     L++   +  +
Sbjct: 75  SAVSASP---SHLRRIMRFLVHQGIF---KEIPTKDGLATGYVNTPLSRRLMITRRDGKS 134

Query: 121 LSPFLLSMLDQLLMKSWQFLSAWLRSDGDRT---PFQTAHGKQFWEHMESESSDREAFNA 180
           L+PF+L      ++  W  LS+ + S  + +   PF   HGK  W   +      +  N 
Sbjct: 135 LAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINE 194

Query: 181 AMASDARLVMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVA 240
           AMA DAR V+  + G    +F+GV  +VDVGGGTG     + + FP ++    DLP V+ 
Sbjct: 195 AMACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIE 254

Query: 241 GLEGNDHNLTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKV 300
             E  D  +  ++GDMF +IP  DA+ +KW+LHDW D++C+KILK CKEA+  N   GKV
Sbjct: 255 VAEVLD-GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPN--IGKV 314

Query: 301 MVLDMVVDHEKKNHMLTE-------TQLFWDVLMMANVG-GKERDEKEWAQLFCEAGFGG 360
           ++++ V+   KK  ++ E        +L  D++MMA+   GKER  KEW  +  EAGF  
Sbjct: 315 LIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFAR 374

[Close]

BLAST of Lag0015270 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-49
Identity = 125/370 (33.78%), Postives = 187/370 (50.54%), Query Frame = 0


Query: 1   MKDTKIAIEGEELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHH--GHPMPLSQLI 60
           M+++K  +  EE  +A   IW ++F F +  A  CA+ L IP+AI +H    P+ LS+L 
Sbjct: 1   MEESKRNLLDEE-AKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELS 60

Query: 61  SALKIDPQKSQHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTNSSLLLLEDNPLTLSPF 120
           SA+   P    H+ R+MR L H G F   + V     +A   TN+ L             
Sbjct: 61  SAVSASP---SHLRRIMRFLVHQGLF---KEVPTKDGLATGYTNTPL------------- 120

Query: 121 LLSMLDQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASDAR 180
                                    R      HGK  W   +      +  N AMA DAR
Sbjct: 121 -----------------------SRRMMITKLHGKDLWAFAQDNLCHSQLINEAMACDAR 180

Query: 181 LVMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGNDH 240
            V+  + G  + +F+GV  +VDVGGGTG     + + FP ++    DLP V+   +  D 
Sbjct: 181 RVVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLD- 240

Query: 241 NLTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDMVV 300
            +  ++GDMF +IP +DA+++KW+LHDW D++C+KILK CKEA++ N   GKV++++ V+
Sbjct: 241 GVENVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPN--IGKVLIVECVI 300

Query: 301 DHEKKNHMLTE--------TQLFWDVLMMANVG-GKERDEKEWAQLFCEAGFGGYKIFPI 360
             EKKN M+ E         +L  D++MM +   GKER  KEW  +  EAGF  Y++   
Sbjct: 301 G-EKKNTMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDF 323

[Close]

BLAST of Lag0015270 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 154.1 bits (388), Expect = 2.2e-37
Identity = 100/321 (31.15%), Postives = 173/321 (53.89%), Query Frame = 0


Query: 31  MALNCAVQLHIPDAISHHGHPMPLSQLISALKI-DPQKSQHIHRLMRIL-AHSGFFLIHE 90
           MAL  A++L + + ++ +G PM  +++ S L   +P+    + R++R+L ++S     + 
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 91  NVE-EHQEVAYSL-TNSSLLLLEDNPLTLSPFLLSMLDQLLMKSWQFLSAWLRSDGDRTP 150
            +  +  E  Y L      L   ++ ++++   L   D++LM+SW  L   +   G   P
Sbjct: 94  KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG--IP 153

Query: 151 FQTAHGKQFWEHMESESSDREAFNAAMASDARLVMSVLLGKYKSVFEGVELLVDVGGGTG 210
           F  A+G   +E+  ++    + FN  M++ + + M  +L  YK  FEG+  LVDVGGG G
Sbjct: 154 FNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIG 213

Query: 211 TMARAIAEAFPQMQCVVLDLPQVVAGLEGNDHNLTFLQGDMFQTIPHADALLLKWILHDW 270
              + I   +P ++ +  DLP V+     +   +  + GDMF ++P  DA+ +KWI HDW
Sbjct: 214 ATLKMIVSKYPNLKGINFDLPHVIEDAPSHP-GIEHVGGDMFVSVPKGDAIFMKWICHDW 273

Query: 271 SDEECVKILKKCKEAIISNGKKGKVMVLDMVVDHEKKNHMLTETQLFWDVLMMA-NVGGK 330
           SDE CVK LK C E++    + GKV++ + ++     + + T+  +  D +M+A N GGK
Sbjct: 274 SDEHCVKFLKNCYESL---PEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGK 333

Query: 331 ERDEKEWAQLFCEAGFGGYKI 347
           ER EKE+  L   +GF G K+
Sbjct: 334 ERTEKEFEALAKASGFKGIKV 347

[Close]

BLAST of Lag0015270 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 146.4 bits (368), Expect = 4.5e-35
Identity = 101/315 (32.06%), Postives = 169/315 (53.65%), Query Frame = 0


Query: 53  PLSQLISALKIDPQKSQHIHRLMRILAHSGFFL--IHENVEEHQEVAYSLTNSSLLLLED 112
           P+     A   +P     I RL+R L         + ++ E  +  AY L      L++D
Sbjct: 56  PVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKD 115

Query: 113 -NPLTLSPFLLSMLDQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAF 172
            +  +++P++L+   +     W +L+  ++ +G  + ++ A+    +E+M+   + ++ F
Sbjct: 116 EDGFSIAPYVLAGCTKAKGGVWSYLTEAIQ-EGGASAWERANEALIFEYMKKNENLKKIF 175

Query: 173 NAAMASDARLVMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQV 232
           N +M +   +VM  +L  Y   FEGV   VDVGG  G+    I   +P ++ +  DLP +
Sbjct: 176 NESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHI 235

Query: 233 VAGLEGNDHNLTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKG 292
           V       H +  + GDMF  IP  + +L+KWILHDW+DE+CV+ILK CK+A+    + G
Sbjct: 236 VKEAP-QIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKAL---PETG 295

Query: 293 KVMVLDMVVDHE-KKNHMLTETQLFWDVLMMA-NVGGKERDEKEWAQLFCEAGFGGYKIF 352
           +++V++M+V  E  +  + T+  L  D+ MM+   GGKER +KE+  L  EAGF   KI 
Sbjct: 296 RIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKI- 355

Query: 353 PILGLRS--LIEIYP 361
            I G  S  +IE+YP
Sbjct: 356 -IYGAYSYWIIELYP 362

[Close]

BLAST of Lag0015270 vs. TAIR 10
Match: AT1G63140.2 (O-methyltransferase family protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.7e-34
Identity = 114/365 (31.23%), Postives = 185/365 (50.68%), Query Frame = 0


Query: 11  EELLEAQAHIWNHIFNFINSMALNCAVQLHIPDAISHHGHPMPLSQLISALKI-----DP 70
           E+ +  QA    H   F   M L  A++L + D I+     + LS    AL +     +P
Sbjct: 26  EDTVSLQAERILHAMTF--PMVLKTALELGVIDMITSVDDGVWLSPSEIALGLPTKPTNP 85

Query: 71  QKSQHIHRLMRILAHSGFFLIHENVEEHQEVAYSLTN---------SSLLLLEDNPLTLS 130
           +    + R++ +LA S   L +  VE    +    T          +  L   D   +L+
Sbjct: 86  EAPVLLDRMLVLLA-SHSILKYRTVETGDNIGSRKTERVYAAEPVCTFFLNRGDGLGSLA 145

Query: 131 PFLLSMLDQLLMKSWQFLSAWLRSDGDRTPFQTAHGKQFWEHMESESSDREAFNAAMASD 190
              + +  ++ MK W+ L   +    D   F +AHG +F+E + S     E FN AM+  
Sbjct: 146 TLFMVLQGEVCMKPWEHLKDMILEGKD--AFTSAHGMRFFELIGSNEQFAEMFNRAMSEA 205

Query: 191 ARLVMSVLLGKYKSVFEGVELLVDVGGGTGTMARAIAEAFPQMQCVVLDLPQVVAGLEGN 250
           + L+M  +L  YK  FE V  LVDVGGG GT+   +   +P ++ +  DL  V+A    N
Sbjct: 206 STLIMKKVLEVYKG-FEDVNTLVDVGGGIGTIIGQVTSKYPHIKGINFDLASVLAHAPFN 265

Query: 251 DHNLTFLQGDMFQTIPHADALLLKWILHDWSDEECVKILKKCKEAIISNGKKGKVMVLDM 310
              +  + GDMF+ IP  DA+ +KWILHDW+DE+CVKILK   +++    +KGKV+++++
Sbjct: 266 -KGVEHVSGDMFKEIPKGDAIFMKWILHDWTDEDCVKILKNYWKSL---PEKGKVIIVEV 325

Query: 311 VVDHEKKNHMLTETQLF-WDVLMMA-NVGGKERDEKEWAQLFCEAGFGGYKIFPILGLRS 360
           V   E K + ++   +F  D+LM+A + GGKER   ++  L  ++GF   +I       S
Sbjct: 326 VTPEEPKINDISSNIVFGMDMLMLAVSSGGKERSLSQFETLASDSGFLRCEIICHAFSYS 380

The following BLAST results are available for this feature:

BLAST of Lag0015270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548392.1	3.6e-159	79.39	probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022991753.1	2.6e-157	77.62	probable O-methyltransferase 3 [Cucurbita maxima]	[more]
KAG6575317.1	3.7e-156	78.21	putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022929871.1	6.4e-156	78.21	probable O-methyltransferase 3 [Cucurbita moschata]	[more]
XP_011659150.1	7.5e-149	72.07	trans-resveratrol di-O-methyltransferase [Cucumis sativus] >KGN44537.1 hypotheti...	[more]

BLAST of Lag0015270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	1.0e-111	60.28	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	1.6e-109	56.45	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
Q6VMW0	3.2e-102	53.35	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=...	[more]
A0A0N9HMN6	5.1e-100	52.69	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]
B0EXJ8	5.0e-95	50.98	Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 ...	[more]

BLAST of Lag0015270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JX64	1.2e-157	77.62	probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111488285 PE=3 ...	[more]
A0A6J1EPC7	3.1e-156	78.21	probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111436348 PE=...	[more]
A0A0A0K881	3.6e-149	72.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G328250 PE=3 SV=1	[more]
A0A6J1DVA5	6.9e-140	70.44	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111023429 PE...	[more]
A0A6J1DTU5	1.9e-137	71.47	trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=...	[more]

BLAST of Lag0015270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	5.2e-55	35.11	O-methyltransferase family protein	[more]
AT4G35150.1	1.1e-49	33.78	O-methyltransferase family protein	[more]
AT5G54160.1	2.2e-37	31.15	O-methyltransferase 1	[more]
AT1G51990.1	4.5e-35	32.06	O-methyltransferase family protein	[more]
AT1G63140.2	1.7e-34	31.23	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 109..360 e-value: 1.8E-85 score: 288.2
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 30..359 e-value: 1.5E-87 score: 291.3
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 8..360
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 198..294 e-value: 6.57515E-4 score: 37.0243
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 31..79 e-value: 1.7E-13 score: 50.4
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 131..341 e-value: 2.1E-58 score: 197.1
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 1..360 e-value: 2.5E-124 score: 412.8
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 8..360
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 21..360 score: 80.393295
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 5..107 e-value: 2.3E-37 score: 128.7
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 112..358
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 11..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015270.1	Lag0015270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity