Lag0015196 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015196
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	S-protein homolog
Location	chr12: 8558476 .. 8558913 (+)
RNA-Seq Expression	Lag0015196
Synteny	Lag0015196

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAATGAGATTGGGATCTATGAAAAAGCACTTTCTTGTTTTCTTGCTTATCTTGTCATTGGCAATTGAGCCAACCAAGGCTGAGGAGCTGAGAAGATGGACAATCCACATTTTGAATGGCCTAAGCAATGACCAAAATTTGTTGGTTCACTGCAAATCCAAAGATAATGATTTGGGCCAACACAATATTAGCGTTGGAACCGATTACAATTGGACTTTTAGAGTAAACTTTTGGAACACGACGTTGTTTTGGTGTTACTTGCAGAAGCCAAATGCACAATCTGTGTCATTCGAAGCCTTTTGGGTTGAGAGTAAATCAATTTGGCTCTTTGATAAATGCTTCGATTCTAACTGTATTTGGACGGCAAAAGATGATGGAATCTATTTGAAAGACAATTCTTCTCAAACAGATGTTTTCGTTCACAAATGGCAGTGA

mRNA sequence

ATGACAATGAGATTGGGATCTATGAAAAAGCACTTTCTTGTTTTCTTGCTTATCTTGTCATTGGCAATTGAGCCAACCAAGGCTGAGGAGCTGAGAAGATGGACAATCCACATTTTGAATGGCCTAAGCAATGACCAAAATTTGTTGGTTCACTGCAAATCCAAAGATAATGATTTGGGCCAACACAATATTAGCGTTGGAACCGATTACAATTGGACTTTTAGAGTAAACTTTTGGAACACGACGTTGTTTTGGTGTTACTTGCAGAAGCCAAATGCACAATCTGTGTCATTCGAAGCCTTTTGGGTTGAGAGTAAATCAATTTGGCTCTTTGATAAATGCTTCGATTCTAACTGTATTTGGACGGCAAAAGATGATGGAATCTATTTGAAAGACAATTCTTCTCAAACAGATGTTTTCGTTCACAAATGGCAGTGA

Coding sequence (CDS)

ATGACAATGAGATTGGGATCTATGAAAAAGCACTTTCTTGTTTTCTTGCTTATCTTGTCATTGGCAATTGAGCCAACCAAGGCTGAGGAGCTGAGAAGATGGACAATCCACATTTTGAATGGCCTAAGCAATGACCAAAATTTGTTGGTTCACTGCAAATCCAAAGATAATGATTTGGGCCAACACAATATTAGCGTTGGAACCGATTACAATTGGACTTTTAGAGTAAACTTTTGGAACACGACGTTGTTTTGGTGTTACTTGCAGAAGCCAAATGCACAATCTGTGTCATTCGAAGCCTTTTGGGTTGAGAGTAAATCAATTTGGCTCTTTGATAAATGCTTCGATTCTAACTGTATTTGGACGGCAAAAGATGATGGAATCTATTTGAAAGACAATTCTTCTCAAACAGATGTTTTCGTTCACAAATGGCAGTGA

Protein sequence

MTMRLGSMKKHFLVFLLILSLAIEPTKAEELRRWTIHILNGLSNDQNLLVHCKSKDNDLGQHNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFWVESKSIWLFDKCFDSNCIWTAKDDGIYLKDNSSQTDVFVHKWQ

Homology

[Close]

BLAST of Lag0015196 vs. NCBI nr
Match: XP_016902699.1 (PREDICTED: uncharacterized protein LOC107991826 [Cucumis melo])

HSP 1 Score: 229.2 bits (583), Expect = 2.3e-56
Identity = 108/146 (73.97%), Postives = 123/146 (84.25%), Query Frame = 0


Query: 2   TMRLGSMKKHFLV--FLLILSLAI-EPTKAEELRRWTIHILNGLSNDQNLLVHCKSKDND 61
           TMR  SMK HFLV  FL +LSLAI +PTKA  L RW IHI+NGLSNDQNL VHC+SKD+D
Sbjct: 28  TMR--SMKNHFLVFCFLFVLSLAIVKPTKAIGLERWHIHIVNGLSNDQNLFVHCQSKDDD 87

Query: 62  LGQHNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFWVESKSIWLFDKCFDSN 121
           LG+  +SVGT++NWTF++NFW+TTLFWCYLQKPNA+SVSFEAFWVE KSIWLF +CF SN
Sbjct: 88  LGKRYLSVGTEFNWTFKINFWDTTLFWCYLQKPNAESVSFEAFWVERKSIWLFYRCFKSN 147

Query: 122 CIWTAKDDGIYLKDNSSQTDVFVHKW 145
           CIWTAKDDGIYLKDN +  D  VH W
Sbjct: 148 CIWTAKDDGIYLKDNPANRDALVHNW 171

[Close]

BLAST of Lag0015196 vs. NCBI nr
Match: XP_022143772.1 (S-protein homolog 74-like [Momordica charantia])

HSP 1 Score: 223.4 bits (568), Expect = 1.3e-54
Identity = 99/145 (68.28%), Postives = 120/145 (82.76%), Query Frame = 0


Query: 3   MRLGSMKKHFLVFLLILSLAI-EPTKA-EELRRWTIHILNGLSNDQNLLVHCKSKDNDLG 62
           M + S KKHFLVFLL+LSL I EP +A  EL++W IH++NGLSN Q L VHCKSKDNDLG
Sbjct: 5   MGVRSTKKHFLVFLLVLSLVILEPIEAFSELKKWQIHVVNGLSNGQTLFVHCKSKDNDLG 64

Query: 63  QHNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFWVESKSIWLFDKCFDSNCI 122
           +HN++ GT++NWTFRVN WNTTLFWCYL KP+ +S SF+ FWVE KSIWLF +C+ SNCI
Sbjct: 65  EHNLNSGTEFNWTFRVNLWNTTLFWCYLHKPDGKSASFDVFWVEKKSIWLFYRCYHSNCI 124

Query: 123 WTAKDDGIYLKDNSSQTDVFVHKWQ 146
           WTAKDDGIYL+DN  Q D+ VH+W+
Sbjct: 125 WTAKDDGIYLRDNPVQRDILVHEWK 149

[Close]

BLAST of Lag0015196 vs. NCBI nr
Match: XP_038896594.1 (S-protein homolog 1-like [Benincasa hispida])

HSP 1 Score: 214.2 bits (544), Expect = 7.7e-52
Identity = 91/144 (63.19%), Postives = 114/144 (79.17%), Query Frame = 0


Query: 3   MRLGSMKKHFLVFLLILSLA-IEPTKAEELRRWTIHILNGLSNDQNLLVHCKSKDNDLGQ 62
           M +  M K  +V   +L LA ++  KA EL +W IH++NGLSN Q L VHCKSKDNDLG+
Sbjct: 1   MEMRCMTKQCVVLFFVLCLAMLDENKAAELAKWQIHVVNGLSNGQILFVHCKSKDNDLGE 60

Query: 63  HNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFWVESKSIWLFDKCFDSNCIW 122
           H +SVG ++NW FRVNFWNTTLFWCYLQKPNAQ  SF+AFW+ES S+WL++ C+DSNCIW
Sbjct: 61  HKLSVGNEFNWRFRVNFWNTTLFWCYLQKPNAQHSSFQAFWIESTSVWLYNMCYDSNCIW 120

Query: 123 TAKDDGIYLKDNSSQTDVFVHKWQ 146
            AKDDG+YLKDN++  DV +HKW+
Sbjct: 121 IAKDDGVYLKDNTNHEDVLIHKWE 144

[Close]

BLAST of Lag0015196 vs. NCBI nr
Match: XP_031745090.1 (S-protein homolog 1-like [Cucumis sativus])

HSP 1 Score: 212.2 bits (539), Expect = 2.9e-51
Identity = 93/145 (64.14%), Postives = 114/145 (78.62%), Query Frame = 0


Query: 3   MRLGSMKKHFLVFLLILSLAI-EPTKAEELRRWTIHILNGLSNDQNLLVHCKSKDNDLGQ 62
           + +G MKK  +V L +L LAI E TKA EL +W IH++NGLSN Q LL HCKSKDNDLG+
Sbjct: 7   IEIGHMKKQCVVLLFVLCLAILEETKAVELEKWHIHVVNGLSNGQILLAHCKSKDNDLGE 66

Query: 63  HNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFWVESKSIWLFDKCFDSNCIW 122
             +  GT++NW FRVNFWNTTLFWCYLQKPN Q  SFE+FW+ES+S+WL+  CF+ NCIW
Sbjct: 67  RRLIAGTEFNWRFRVNFWNTTLFWCYLQKPNGQHSSFESFWIESRSVWLYTMCFEKNCIW 126

Query: 123 TAKDDGIYLKDN-SSQTDVFVHKWQ 146
           TAKDDGIYLKDN  +  D+ +HKW+
Sbjct: 127 TAKDDGIYLKDNFDTHKDILIHKWE 151

[Close]

BLAST of Lag0015196 vs. NCBI nr
Match: XP_022143780.1 (S-protein homolog 1-like [Momordica charantia])

HSP 1 Score: 207.6 bits (527), Expect = 7.2e-50
Identity = 93/140 (66.43%), Postives = 110/140 (78.57%), Query Frame = 0


Query: 6   GSMKKHFLVFLLILSLAI-EPTKAEELRRWTIHILNGLSNDQNLLVHCKSKDNDLGQHNI 65
           G   KHFLVFL + SLAI E  +A  L +W IH+ N LSN Q L VHCKSK++DLG+HN+
Sbjct: 3   GRYPKHFLVFLFVSSLAIVEQIEAVPLSKWQIHVRNELSNTQMLFVHCKSKNDDLGEHNL 62

Query: 66  SVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFWVESKSIWLFDKCFDSNCIWTAK 125
           SVGT++NW FRVN W+TTL+WCYLQKPN QSVSF+AFWVE  SIWL+ KC +SNC W AK
Sbjct: 63  SVGTEFNWRFRVNIWDTTLYWCYLQKPNGQSVSFDAFWVEKDSIWLYYKCLESNCTWKAK 122

Query: 126 DDGIYLKDNSSQTDVFVHKW 145
           DDGIYL++N    DVFVHKW
Sbjct: 123 DDGIYLRNNPDGRDVFVHKW 142

[Close]

BLAST of Lag0015196 vs. ExPASy Swiss-Prot
Match: F4JLS0 (S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.3e-22
Identity = 45/116 (38.79%), Postives = 71/116 (61.21%), Query Frame = 0


Query: 30  ELRRWTIHILNGLSNDQNLLVHCKSKDNDLGQHNISVGTDYNWTFRVNFWNTTLFWCYLQ 89
           ++  W + ++NGL+  + L +HCKSK++DLG+ N+     ++W F  N  ++T FWCY+ 
Sbjct: 37  KISEWQVTVVNGLTTGETLFIHCKSKEDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMN 96

Query: 90  KPNAQSVSFEAFWVESKSIWLFDKCFDSNCIWTAKDDGIYLKDNSSQTDVFVHKWQ 146
           K N   ++   FW     + LF +C   NCIWTAK DG+YL +++S  DV   KW+
Sbjct: 97  KDNGH-MNVNVFW---DDVILFHRCGWKNCIWTAKTDGLYLWNSASGEDVLSRKWE 148

[Close]

BLAST of Lag0015196 vs. ExPASy Swiss-Prot
Match: Q2HQ46 (S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.9e-21
Identity = 44/116 (37.93%), Postives = 71/116 (61.21%), Query Frame = 0


Query: 30  ELRRWTIHILNGLSNDQNLLVHCKSKDNDLGQHNISVGTDYNWTFRVNFWNTTLFWCYLQ 89
           ++  W + + NGL+  + L +HCKSK+NDLG  N+     ++W F  N  ++TLFWCY+ 
Sbjct: 37  KISEWQVTVANGLTTGETLFIHCKSKENDLGDINLKFLDRFSWNFGENMLHSTLFWCYMS 96

Query: 90  KPNAQSVSFEAFWVESKSIWLFDKCFDSNCIWTAKDDGIYLKDNSSQTDVFVHKWQ 146
           K +   ++ + FW     + LF +C   NC+WTAK+DG+YL +++   DV   KW+
Sbjct: 97  KDDGH-MNVKVFW---DDVILFHRCDWKNCVWTAKNDGLYLWNSAIGEDVLSEKWK 148

[Close]

BLAST of Lag0015196 vs. ExPASy Swiss-Prot
Match: Q9FI84 (S-protein homolog 27 OS=Arabidopsis thaliana OX=3702 GN=SPH27 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.2e-12
Identity = 40/102 (39.22%), Postives = 51/102 (50.00%), Query Frame = 0


Query: 43  SNDQNLLVHCKSKDNDLGQHNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFW 102
           +ND  L +HCKSKD+DLG H    G  Y W F VNF N+TL++C   +       F+   
Sbjct: 54  NNDYLLGIHCKSKDDDLGFHIHKEGELYGWKFHVNFQNSTLYFCGFSQGQDNKGVFDIDR 113

Query: 103 VESKSIWLFDKCFDSNCIWTAKDDGIYLKDNSSQTDVFVHKW 145
            E      F +C   NC W AK D +Y   N  QT  +  KW
Sbjct: 114 AERD----FYRC--RNCTWNAKKDSLYGYSNLPQTVTWFFKW 149

[Close]

BLAST of Lag0015196 vs. ExPASy Swiss-Prot
Match: F2Q9V4 (S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 1.4e-11
Identity = 37/101 (36.63%), Postives = 54/101 (53.47%), Query Frame = 0


Query: 44  NDQNLLVHCKSKDNDLGQHNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQSVSFEAFWV 103
           ND  L VHCKS+D+D G H +  G  Y W F VNF N+TL++C   +   +   F+ +  
Sbjct: 40  NDYLLGVHCKSRDDDHGFHILQKGGLYGWMFYVNFMNSTLYFCGFSQEQVKKGVFDIY-- 99

Query: 104 ESKSIWLFDKCFDSNCIWTAKDDGIYLKDNSSQTDVFVHKW 145
             K++    +C   NC W AK+DGIY      + +   +KW
Sbjct: 100 --KAVRDSSRC--RNCTWEAKEDGIYGYGEIPKKNPLFYKW 134

[Close]

BLAST of Lag0015196 vs. ExPASy Swiss-Prot
Match: Q40975 (Self-incompatibility protein S1 OS=Papaver rhoeas OX=33128 GN=S1 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-10
Identity = 33/101 (32.67%), Postives = 54/101 (53.47%), Query Frame = 0


Query: 36  IHILNGLSNDQNLLVHCKSKDNDLGQHNISVGTDYNWTFRVNFWNTTLFWCYLQKPNAQS 95
           + I+N   N +++ +HC+SKDNDL    ++ G D +++FR +F++TT F+C LQ      
Sbjct: 26  VRIMNRRGNGRSIGIHCRSKDNDLQNQTVTSGHDMSFSFREDFFHTTHFYCDLQWDKETK 85

Query: 96  VSFEAFWVESKSIWLFDKCFDSNCIWTAKDDGIYLKDNSSQ 137
             F ++  +       D    S C+W   DDG+Y  D   Q
Sbjct: 86  FGFYSYQAKRDD----DGRCSSQCLWKIMDDGLYGFDQEHQ 122

The following BLAST results are available for this feature:

BLAST of Lag0015196 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016902699.1	2.3e-56	73.97	PREDICTED: uncharacterized protein LOC107991826 [Cucumis melo]	[more]
XP_022143772.1	1.3e-54	68.28	S-protein homolog 74-like [Momordica charantia]	[more]
XP_038896594.1	7.7e-52	63.19	S-protein homolog 1-like [Benincasa hispida]	[more]
XP_031745090.1	2.9e-51	64.14	S-protein homolog 1-like [Cucumis sativus]	[more]
XP_022143780.1	7.2e-50	66.43	S-protein homolog 1-like [Momordica charantia]	[more]

BLAST of Lag0015196 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JLS0	2.3e-22	38.79	S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1	[more]
Q2HQ46	1.9e-21	37.93	S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1	[more]
Q9FI84	6.2e-12	39.22	S-protein homolog 27 OS=Arabidopsis thaliana OX=3702 GN=SPH27 PE=2 SV=1	[more]
F2Q9V4	1.4e-11	36.63	S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2	[more]
Q40975	1.5e-10	32.67	Self-incompatibility protein S1 OS=Papaver rhoeas OX=33128 GN=S1 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010264	Plant self-incompatibility S1	PFAM	PF05938	Self-incomp_S1	coord: 35..144 e-value: 3.0E-28 score: 98.4
IPR010264	Plant self-incompatibility S1	PANTHER	PTHR31232	FAMILY NOT NAMED	coord: 11..144
None	No IPR available	PANTHER	PTHR31232:SF99	SUBFAMILY NOT NAMED	coord: 11..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015196.1	Lag0015196.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060320	rejection of self pollen
cellular_component	GO:0005576	extracellular region