Lag0015195 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0015195 vs. NCBI nr
Match: XP_022968914.1 (GDSL esterase/lipase At2g04570-like [Cucurbita maxima])

HSP 1 Score: 642.9 bits (1657), Expect = 1.6e-180
Identity = 315/352 (89.49%), Postives = 331/352 (94.03%), Query Frame = 0

Query: 1   MAYMFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60
           M YM FLCLL+   LL+SSP+TI EAKVSA+IVFGDSSVDAGNNNFIPTIARSNFLPYGR
Sbjct: 1   MPYMAFLCLLLLVSLLLSSPRTITEAKVSAVIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60

Query: 61  DFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNA 120
           DF GGKATGRFSNGRIPTDFISEAFGLKP VPAYLDPAYNISD A+GVTFASAGTGYDNA
Sbjct: 61  DFVGGKATGRFSNGRIPTDFISEAFGLKPTVPAYLDPAYNISDFASGVTFASAGTGYDNA 120

Query: 121 TSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPG 180
           TS+VLSVI LWKQLE YKEYQAKL AYQGSATAN+T+ EALYVMSLGTNDFLENYYTMPG
Sbjct: 121 TSDVLSVIPLWKQLEYYKEYQAKLIAYQGSATANETMKEALYVMSLGTNDFLENYYTMPG 180

Query: 181 RSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVE 240
           RSSQYNIQQYQDFL+GIA GFIEKLHGLGARKISLGGLPPMGCLPLERT NVFG NDC+E
Sbjct: 181 RSSQYNIQQYQDFLIGIAGGFIEKLHGLGARKISLGGLPPMGCLPLERTRNVFGSNDCME 240

Query: 241 SYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCAT 300
           SYNNLAV+FNKKL+ALTVKLN DLP+I+LVFSNPYYVLL  IKKPSLYGFDVTSTACCAT
Sbjct: 241 SYNNLAVDFNKKLEALTVKLNKDLPDIELVFSNPYYVLLQAIKKPSLYGFDVTSTACCAT 300

Query: 301 GMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           G+FEMGYACNRNS+FTC DANKYIFWDSFHPTQKTNQIVS YVVKNVLSQFL
Sbjct: 301 GIFEMGYACNRNSLFTCTDANKYIFWDSFHPTQKTNQIVSGYVVKNVLSQFL 352

BLAST of Lag0015195 vs. NCBI nr
Match: XP_023553654.1 (GDSL esterase/lipase At2g04570-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 640.6 bits (1651), Expect = 8.1e-180
Identity = 314/352 (89.20%), Postives = 331/352 (94.03%), Query Frame = 0

Query: 1   MAYMFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60
           M +M FLCLL+   LL+SSP+TI EAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR
Sbjct: 1   MTHMAFLCLLLIVSLLLSSPRTITEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60

Query: 61  DFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNA 120
           DF GGKATGRFSNGRIPTDFISEAFGLKP VPAYLDPAYNISD A+GVTFASAGTGYDNA
Sbjct: 61  DFVGGKATGRFSNGRIPTDFISEAFGLKPTVPAYLDPAYNISDFASGVTFASAGTGYDNA 120

Query: 121 TSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPG 180
           TS+VLSVI LWKQLE YKEYQAKL AYQGSATAN+T+ EALYVMSLGTNDFLENYYTMPG
Sbjct: 121 TSDVLSVIPLWKQLEYYKEYQAKLIAYQGSATANETMKEALYVMSLGTNDFLENYYTMPG 180

Query: 181 RSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVE 240
           RSSQYNIQQYQDFL+GIA GFIEKLHGLGARKISLGGLPPMGCLPLERT NVFG NDC+E
Sbjct: 181 RSSQYNIQQYQDFLIGIAGGFIEKLHGLGARKISLGGLPPMGCLPLERTRNVFGSNDCME 240

Query: 241 SYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCAT 300
           SYNNLAV+FNKKL+ALTVKLN DLP+I+LVFSNPYYVLL  IKKPSLYGFDVTSTACCAT
Sbjct: 241 SYNNLAVDFNKKLEALTVKLNKDLPDIELVFSNPYYVLLQAIKKPSLYGFDVTSTACCAT 300

Query: 301 GMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           G+FEMGYACNRN++FTC DANKYIFWDSFHPTQKTNQIVS YVVKNVLSQFL
Sbjct: 301 GIFEMGYACNRNNLFTCTDANKYIFWDSFHPTQKTNQIVSGYVVKNVLSQFL 352

BLAST of Lag0015195 vs. NCBI nr
Match: XP_022952099.1 (GDSL esterase/lipase At2g04570-like [Cucurbita moschata])

HSP 1 Score: 636.7 bits (1641), Expect = 1.2e-178
Identity = 314/352 (89.20%), Postives = 330/352 (93.75%), Query Frame = 0

Query: 1   MAYMFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60
           M +M FLCLL+   LL+SSP+TI EAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR
Sbjct: 1   MPHMAFLCLLLIVSLLLSSPRTITEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60

Query: 61  DFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNA 120
           DF GGKATGRFSNGRIPTDFISEAFGLKP VPAYLDPAYNISD A+GVTFASAGTGYDNA
Sbjct: 61  DFVGGKATGRFSNGRIPTDFISEAFGLKPTVPAYLDPAYNISDFASGVTFASAGTGYDNA 120

Query: 121 TSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPG 180
           TS+VLSVI LWKQLE YKEYQAKL AYQGSATAN+TI EALYVMSLGTNDFLENYYTMPG
Sbjct: 121 TSDVLSVIPLWKQLEYYKEYQAKLIAYQGSATANETIKEALYVMSLGTNDFLENYYTMPG 180

Query: 181 RSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVE 240
           RSSQYNIQQYQDFL+GIA GFIEKLHGLGARKISLGGLPPMGCLPLERT NVFG NDC+E
Sbjct: 181 RSSQYNIQQYQDFLIGIAGGFIEKLHGLGARKISLGGLPPMGCLPLERTRNVFGSNDCME 240

Query: 241 SYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCAT 300
           SYNNLAV+FNKKL+ALTVKLN DLP+I+LVFSNPY VLL  IKKPSLYGFDVTSTACCAT
Sbjct: 241 SYNNLAVDFNKKLEALTVKLNKDLPDIELVFSNPYDVLLQAIKKPSLYGFDVTSTACCAT 300

Query: 301 GMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           G+FEMGYACNRNS+FTC DANKYIFWDSFHPTQKTNQIVS YVVK+VLSQFL
Sbjct: 301 GIFEMGYACNRNSLFTCTDANKYIFWDSFHPTQKTNQIVSGYVVKSVLSQFL 352

BLAST of Lag0015195 vs. NCBI nr
Match: KAG7012023.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 636.3 bits (1640), Expect = 1.5e-178
Identity = 312/349 (89.40%), Postives = 327/349 (93.70%), Query Frame = 0

Query: 4   MFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFA 63
           M FLCL +   LL+SSP+TI EAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDF 
Sbjct: 1   MAFLCLFLIVSLLLSSPRTITEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFV 60

Query: 64  GGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSN 123
           GGKATGRFSNGRIPTDFISEAFGLKP VPAYLDPAYNISD A+GVTFASAGTGYDNATS+
Sbjct: 61  GGKATGRFSNGRIPTDFISEAFGLKPTVPAYLDPAYNISDFASGVTFASAGTGYDNATSD 120

Query: 124 VLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSS 183
           VLSVI LWKQLE YKEYQAKL AYQGSATAN+T+ EALYVMSLGTNDFLENYYTMPGRSS
Sbjct: 121 VLSVIPLWKQLEYYKEYQAKLIAYQGSATANETMKEALYVMSLGTNDFLENYYTMPGRSS 180

Query: 184 QYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYN 243
           QYNIQQYQDFL+GI  GFIEKLHGLGARKISLGGLPPMGCLPLERT NVFG NDC+ESYN
Sbjct: 181 QYNIQQYQDFLIGIVGGFIEKLHGLGARKISLGGLPPMGCLPLERTRNVFGSNDCMESYN 240

Query: 244 NLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMF 303
           NLAV+FNKKL+ALTVKLN DLP+I+LVFSNPYYVLL  IKKPSLYGFDVTSTACCATG+F
Sbjct: 241 NLAVDFNKKLEALTVKLNKDLPDIELVFSNPYYVLLQAIKKPSLYGFDVTSTACCATGIF 300

Query: 304 EMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           EMGYACNRNS+FTC DANKYIFWDSFHPTQKTNQIVS YVVKNVLSQFL
Sbjct: 301 EMGYACNRNSLFTCTDANKYIFWDSFHPTQKTNQIVSGYVVKNVLSQFL 349

BLAST of Lag0015195 vs. NCBI nr
Match: XP_038887013.1 (GDSL esterase/lipase At2g04570-like [Benincasa hispida])

HSP 1 Score: 634.4 bits (1635), Expect = 5.8e-178
Identity = 312/352 (88.64%), Postives = 329/352 (93.47%), Query Frame = 0

Query: 1   MAYMFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60
           MAYM FLC L    LL SSPKTI+EAKV A+IVFGDSSVDAGNNNFIPTIARSNFLPYGR
Sbjct: 1   MAYMVFLCSLTIVSLL-SSPKTIIEAKVPAVIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60

Query: 61  DFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNA 120
           DFAGGKATGRFSNGRIPTDFISEAFGLK  +PAYLDP YNISDLATGVTFASAGTGYDNA
Sbjct: 61  DFAGGKATGRFSNGRIPTDFISEAFGLKSTIPAYLDPTYNISDLATGVTFASAGTGYDNA 120

Query: 121 TSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPG 180
           TS+VLSVI LWKQLE YKEYQAKL AY+GS+TAN+TI EALYVMSLGTNDFLENYYTMPG
Sbjct: 121 TSDVLSVIPLWKQLEYYKEYQAKLIAYEGSSTANETIKEALYVMSLGTNDFLENYYTMPG 180

Query: 181 RSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVE 240
           RSSQYNIQQYQDFLVGIA GFIEKLH LGARKISLGGLPPMGCLPLERT N+FGGNDC+E
Sbjct: 181 RSSQYNIQQYQDFLVGIAGGFIEKLHSLGARKISLGGLPPMGCLPLERTRNLFGGNDCLE 240

Query: 241 SYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCAT 300
           SYNNLA++FN KLKALTVKLN DLP+++LVFSNPYY+LL MIKKPSLYGFDVTSTACCAT
Sbjct: 241 SYNNLAMDFNNKLKALTVKLNKDLPDVELVFSNPYYILLSMIKKPSLYGFDVTSTACCAT 300

Query: 301 GMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           GMFEMGYACNRNSMFTC DANKYIFWDSFHPTQ+TNQ+VS YVVKNVLSQFL
Sbjct: 301 GMFEMGYACNRNSMFTCTDANKYIFWDSFHPTQRTNQLVSNYVVKNVLSQFL 351

BLAST of Lag0015195 vs. ExPASy Swiss-Prot
Match: Q9SJB4 (GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 5.3e-134
Identity = 226/338 (66.86%), Postives = 271/338 (80.18%), Query Frame = 0

Query: 16  LISSPKTIVEA-KVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGGKATGRFSNG 75
           LI+   T+  A K+ AIIVFGDSSVDAGNNN+IPT+ARSNF PYGRDF GGK TGRF NG
Sbjct: 13  LIAMSSTVTFAGKIPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNG 72

Query: 76  RIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVLSVISLWKQL 135
           +I TDF+SEA GLKPI+PAYLDP+YNISD ATGVTFASA TGYDNATS+VLSV+ LWKQL
Sbjct: 73  KIATDFMSEALGLKPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQL 132

Query: 136 ENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQYNIQQYQDFL 195
           E YKEYQ KL+AYQG     +TI  +LY++S+GTNDFLENY+  PGRSSQY++  YQDFL
Sbjct: 133 EYYKEYQTKLKAYQGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFL 192

Query: 196 VGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYNNLAVEFNKKLK 255
            GIA  F++KLHGLGARKISLGGLPPMGC+PLER TN+  G +CV  YN++AV+FN KL 
Sbjct: 193 AGIAKEFVKKLHGLGARKISLGGLPPMGCMPLERATNIGTGGECVGRYNDIAVQFNSKLD 252

Query: 256 ALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMFEMGYACNRNSM 315
            +  KL+ +LP  +LVFSNPY   + +IK PS +GF+V   ACCATGMFEMGY C RN+ 
Sbjct: 253 KMVEKLSKELPGSNLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNP 312

Query: 316 FTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           FTC +A+KY+FWDSFHPTQKTN I++  ++ +    FL
Sbjct: 313 FTCTNADKYVFWDSFHPTQKTNHIMANALMNSTFPHFL 350

BLAST of Lag0015195 vs. ExPASy Swiss-Prot
Match: Q67ZI9 (GDSL esterase/lipase At2g42990 OS=Arabidopsis thaliana OX=3702 GN=At2g42990 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.4e-120
Identity = 200/337 (59.35%), Postives = 267/337 (79.23%), Query Frame = 0

Query: 15  LLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGGKATGRFSNG 74
           +++++  +I  AK+ AIIVFGDSSVD+GNNNFI T+AR+NF PYGRDF GG+ATGRF NG
Sbjct: 13  IILTTLVSIAGAKIPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNG 72

Query: 75  RIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVLSVISLWKQL 134
           R+ +DF SEA+GLKP VPAYLDP+YNISD ATGV FASAGTGYDN+T++VL VI LWK++
Sbjct: 73  RLSSDFTSEAYGLKPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEV 132

Query: 135 ENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQYNIQQYQDFL 194
           E +KEYQ+ L AY G   A + I E+LY++S+GTNDFLENYYT+P R SQ++I QYQDFL
Sbjct: 133 EYFKEYQSNLSAYLGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFL 192

Query: 195 VGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYNNLAVEFNKKLK 254
           V IA  F++ ++ LGARK+S  G+ PMGCLPLER TN+     C  SYN+LAV+FN +L+
Sbjct: 193 VEIAEVFLKDIYRLGARKMSFTGISPMGCLPLERVTNLDDPFSCARSYNDLAVDFNGRLR 252

Query: 255 ALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMFEMGYACNRNSM 314
            L  KLN +L  I + F+NPY ++  ++ KP+LYG +++S+ACC TG+FEMG+ C +++ 
Sbjct: 253 RLVTKLNRELTGIKIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNP 312

Query: 315 FTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQF 352
            TC DANK++FWD+FHPT++TNQIVS +  K++ + F
Sbjct: 313 LTCSDANKFVFWDAFHPTERTNQIVSDHFFKHLKNLF 349

BLAST of Lag0015195 vs. ExPASy Swiss-Prot
Match: Q8VY93 (GDSL esterase/lipase At4g26790 OS=Arabidopsis thaliana OX=3702 GN=At4g26790 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 5.9e-117
Identity = 205/343 (59.77%), Postives = 262/343 (76.38%), Query Frame = 0

Query: 9   LLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGGKAT 68
           LL+AA LL+  P+T   AK  A+IVFGDS+VD+GNNN I T+ +SNF PYGRD+  GKAT
Sbjct: 10  LLLAAQLLVKIPETC--AKFPALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKAT 69

Query: 69  GRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVLSVI 128
           GRFSNGRI  DFISE  GLK  VPAYLDPAYNI+D ATGV FASAGTG DNATS VLSV+
Sbjct: 70  GRFSNGRIAPDFISEGLGLKNAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVLSVM 129

Query: 129 SLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQYNIQ 188
            LWK++E YKEYQ +LR+Y G   AN+ I+E+LY++S+GTNDFLENYY +P +  +Y++ 
Sbjct: 130 PLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKYSVN 189

Query: 189 QYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYNNLAVE 248
           +YQ FL+GIAA F+  ++ LGARK+SL GL P GCLPLERTT +F G+ C+E YN +A +
Sbjct: 190 EYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQLFYGSKCIEEYNIVARD 249

Query: 249 FNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMFEMGYA 308
           FN K++    +LN DL  I LVFSNPY ++  +I  P  +GF+   +ACC TG +EM Y 
Sbjct: 250 FNIKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENVRSACCGTGYYEMSYL 309

Query: 309 CNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQF 352
           C++ + FTC DA+KY+FWDSFHPT+KTN IV+ +V+K  LS+F
Sbjct: 310 CDKMNPFTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRF 350

BLAST of Lag0015195 vs. ExPASy Swiss-Prot
Match: Q3ECM4 (GDSL esterase/lipase At1g58725 OS=Arabidopsis thaliana OX=3702 GN=At1g58725 PE=2 SV=2)

HSP 1 Score: 276.6 bits (706), Expect = 4.0e-73
Identity = 142/350 (40.57%), Postives = 212/350 (60.57%), Query Frame = 0

Query: 9   LLIAAHLLISSPKTIVEAK---VSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGG 68
           LL A  L+        + K   + A+IVFGDS +D GNNN +PT+ + NF PYG+D+ GG
Sbjct: 6   LLFALVLIFVEANAATQGKNTTIPALIVFGDSIMDTGNNNNLPTLLKCNFPPYGKDYPGG 65

Query: 69  KATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVL 128
            ATGRFS+GR+P+D I+E  GL   +PAY++P     DL  GVTFAS GTGYD  T+ ++
Sbjct: 66  FATGRFSDGRVPSDLIAEKLGLAKTLPAYMNPYLKPEDLLKGVTFASGGTGYDPLTAKIM 125

Query: 129 SVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQY 188
           SVIS+W QL N+KEY +K++ + G   A   +  + +++   +ND    Y     ++ +Y
Sbjct: 126 SVISVWDQLINFKEYISKIKRHFGEEKAKDILEHSFFLVVSSSNDLAHTYL---AQTHRY 185

Query: 189 NIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGG---NDCVESY 248
           +   Y +FL   A  F+ +LH LGARKI +    P+GC+PL+RT  VFGG     C +  
Sbjct: 186 DRTSYANFLADSAVHFVRELHKLGARKIGVFSAVPVGCVPLQRT--VFGGFFTRGCNQPL 245

Query: 249 NNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGM 308
           NN+A +FN +L      L+ +L  + +++ N Y  L  MI+ P  YGF+V    CC  G+
Sbjct: 246 NNMAKQFNARLSPALDSLDKELDGV-ILYINVYDTLFDMIQHPKKYGFEVADRGCCGKGL 305

Query: 309 FEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
             + Y CN  + FTC +++ YIFWDS+HP+++  Q+    +V N+L ++L
Sbjct: 306 LAISYLCNSLNPFTCSNSSAYIFWDSYHPSERAYQV----IVDNLLDKYL 345

BLAST of Lag0015195 vs. ExPASy Swiss-Prot
Match: F4IBF0 (GDSL esterase/lipase At1g59030 OS=Arabidopsis thaliana OX=3702 GN=At1g59030 PE=3 SV=2)

HSP 1 Score: 276.6 bits (706), Expect = 4.0e-73
Identity = 142/350 (40.57%), Postives = 212/350 (60.57%), Query Frame = 0

Query: 9   LLIAAHLLISSPKTIVEAK---VSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGG 68
           LL A  L+        + K   + A+IVFGDS +D GNNN +PT+ + NF PYG+D+ GG
Sbjct: 6   LLFALVLIFVEANAATQGKNTTIPALIVFGDSIMDTGNNNNLPTLLKCNFPPYGKDYPGG 65

Query: 69  KATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVL 128
            ATGRFS+GR+P+D I+E  GL   +PAY++P     DL  GVTFAS GTGYD  T+ ++
Sbjct: 66  FATGRFSDGRVPSDLIAEKLGLAKTLPAYMNPYLKPEDLLKGVTFASGGTGYDPLTAKIM 125

Query: 129 SVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQY 188
           SVIS+W QL N+KEY +K++ + G   A   +  + +++   +ND    Y     ++ +Y
Sbjct: 126 SVISVWDQLINFKEYISKIKRHFGEEKAKDILEHSFFLVVSSSNDLAHTYL---AQTHRY 185

Query: 189 NIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGG---NDCVESY 248
           +   Y +FL   A  F+ +LH LGARKI +    P+GC+PL+RT  VFGG     C +  
Sbjct: 186 DRTSYANFLADSAVHFVRELHKLGARKIGVFSAVPVGCVPLQRT--VFGGFFTRGCNQPL 245

Query: 249 NNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGM 308
           NN+A +FN +L      L+ +L  + +++ N Y  L  MI+ P  YGF+V    CC  G+
Sbjct: 246 NNMAKQFNARLSPALDSLDKELDGV-ILYINVYDTLFDMIQHPKKYGFEVADRGCCGKGL 305

Query: 309 FEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
             + Y CN  + FTC +++ YIFWDS+HP+++  Q+    +V N+L ++L
Sbjct: 306 LAISYLCNSLNPFTCSNSSAYIFWDSYHPSERAYQV----IVDNLLDKYL 345

BLAST of Lag0015195 vs. ExPASy TrEMBL
Match: A0A6J1I114 (GDSL esterase/lipase At2g04570-like OS=Cucurbita maxima OX=3661 GN=LOC111468087 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.9e-181
Identity = 315/352 (89.49%), Postives = 331/352 (94.03%), Query Frame = 0

Query: 1   MAYMFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60
           M YM FLCLL+   LL+SSP+TI EAKVSA+IVFGDSSVDAGNNNFIPTIARSNFLPYGR
Sbjct: 1   MPYMAFLCLLLLVSLLLSSPRTITEAKVSAVIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60

Query: 61  DFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNA 120
           DF GGKATGRFSNGRIPTDFISEAFGLKP VPAYLDPAYNISD A+GVTFASAGTGYDNA
Sbjct: 61  DFVGGKATGRFSNGRIPTDFISEAFGLKPTVPAYLDPAYNISDFASGVTFASAGTGYDNA 120

Query: 121 TSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPG 180
           TS+VLSVI LWKQLE YKEYQAKL AYQGSATAN+T+ EALYVMSLGTNDFLENYYTMPG
Sbjct: 121 TSDVLSVIPLWKQLEYYKEYQAKLIAYQGSATANETMKEALYVMSLGTNDFLENYYTMPG 180

Query: 181 RSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVE 240
           RSSQYNIQQYQDFL+GIA GFIEKLHGLGARKISLGGLPPMGCLPLERT NVFG NDC+E
Sbjct: 181 RSSQYNIQQYQDFLIGIAGGFIEKLHGLGARKISLGGLPPMGCLPLERTRNVFGSNDCME 240

Query: 241 SYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCAT 300
           SYNNLAV+FNKKL+ALTVKLN DLP+I+LVFSNPYYVLL  IKKPSLYGFDVTSTACCAT
Sbjct: 241 SYNNLAVDFNKKLEALTVKLNKDLPDIELVFSNPYYVLLQAIKKPSLYGFDVTSTACCAT 300

Query: 301 GMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           G+FEMGYACNRNS+FTC DANKYIFWDSFHPTQKTNQIVS YVVKNVLSQFL
Sbjct: 301 GIFEMGYACNRNSLFTCTDANKYIFWDSFHPTQKTNQIVSGYVVKNVLSQFL 352

BLAST of Lag0015195 vs. ExPASy TrEMBL
Match: A0A6J1GKM6 (GDSL esterase/lipase At2g04570-like OS=Cucurbita moschata OX=3662 GN=LOC111454860 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.6e-179
Identity = 314/352 (89.20%), Postives = 330/352 (93.75%), Query Frame = 0

Query: 1   MAYMFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60
           M +M FLCLL+   LL+SSP+TI EAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR
Sbjct: 1   MPHMAFLCLLLIVSLLLSSPRTITEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGR 60

Query: 61  DFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNA 120
           DF GGKATGRFSNGRIPTDFISEAFGLKP VPAYLDPAYNISD A+GVTFASAGTGYDNA
Sbjct: 61  DFVGGKATGRFSNGRIPTDFISEAFGLKPTVPAYLDPAYNISDFASGVTFASAGTGYDNA 120

Query: 121 TSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPG 180
           TS+VLSVI LWKQLE YKEYQAKL AYQGSATAN+TI EALYVMSLGTNDFLENYYTMPG
Sbjct: 121 TSDVLSVIPLWKQLEYYKEYQAKLIAYQGSATANETIKEALYVMSLGTNDFLENYYTMPG 180

Query: 181 RSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVE 240
           RSSQYNIQQYQDFL+GIA GFIEKLHGLGARKISLGGLPPMGCLPLERT NVFG NDC+E
Sbjct: 181 RSSQYNIQQYQDFLIGIAGGFIEKLHGLGARKISLGGLPPMGCLPLERTRNVFGSNDCME 240

Query: 241 SYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCAT 300
           SYNNLAV+FNKKL+ALTVKLN DLP+I+LVFSNPY VLL  IKKPSLYGFDVTSTACCAT
Sbjct: 241 SYNNLAVDFNKKLEALTVKLNKDLPDIELVFSNPYDVLLQAIKKPSLYGFDVTSTACCAT 300

Query: 301 GMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           G+FEMGYACNRNS+FTC DANKYIFWDSFHPTQKTNQIVS YVVK+VLSQFL
Sbjct: 301 GIFEMGYACNRNSLFTCTDANKYIFWDSFHPTQKTNQIVSGYVVKSVLSQFL 352

BLAST of Lag0015195 vs. ExPASy TrEMBL
Match: A0A5D3C3R2 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00020 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.5e-174
Identity = 310/353 (87.82%), Postives = 324/353 (91.78%), Query Frame = 0

Query: 2   AYMFFLCLL--IAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYG 61
           AY  FLC L  I    L SSPKTI EAKV A+IVFGDSSVDAGNNNFIPTIARSNF PYG
Sbjct: 3   AYNVFLCFLTIIVPFHLSSSPKTITEAKVPAVIVFGDSSVDAGNNNFIPTIARSNFFPYG 62

Query: 62  RDFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDN 121
           RDFAGGKATGRFSNGRIPTDFISEAFGLKP +PAYLDPAYNISDLATG+TFASAGTGYDN
Sbjct: 63  RDFAGGKATGRFSNGRIPTDFISEAFGLKPTIPAYLDPAYNISDLATGLTFASAGTGYDN 122

Query: 122 ATSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMP 181
           ATSNVLSVI LWKQLE YKEYQAKL AYQGS+TAN+TI EALYVMSLGTNDFLENYYTMP
Sbjct: 123 ATSNVLSVIPLWKQLEYYKEYQAKLTAYQGSSTANETIKEALYVMSLGTNDFLENYYTMP 182

Query: 182 GRSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCV 241
           GRSSQY+IQQYQDFLVGIA+GFIEKL+ LGARKISLGGLPPMGCLPLERT NVFGG DC+
Sbjct: 183 GRSSQYSIQQYQDFLVGIASGFIEKLYSLGARKISLGGLPPMGCLPLERTRNVFGGYDCL 242

Query: 242 ESYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCA 301
           ESYNN+AV+FN KLKALTVKLN DLP I LVFSNPY VL+ MIKKPSLYGFDVTSTACCA
Sbjct: 243 ESYNNVAVDFNNKLKALTVKLNRDLPGIQLVFSNPYDVLMSMIKKPSLYGFDVTSTACCA 302

Query: 302 TGMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           TGMFEMGYACNR SMFTC DANKYIFWDSFHPTQKTNQ+VS+YVVKNVLSQFL
Sbjct: 303 TGMFEMGYACNRESMFTCTDANKYIFWDSFHPTQKTNQLVSSYVVKNVLSQFL 355

BLAST of Lag0015195 vs. ExPASy TrEMBL
Match: A0A1S3BM52 (GDSL esterase/lipase At2g04570 OS=Cucumis melo OX=3656 GN=LOC103491362 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.5e-174
Identity = 310/353 (87.82%), Postives = 324/353 (91.78%), Query Frame = 0

Query: 2   AYMFFLCLL--IAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYG 61
           AY  FLC L  I    L SSPKTI EAKV A+IVFGDSSVDAGNNNFIPTIARSNF PYG
Sbjct: 3   AYNVFLCFLTIIVPFHLSSSPKTITEAKVPAVIVFGDSSVDAGNNNFIPTIARSNFFPYG 62

Query: 62  RDFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDN 121
           RDFAGGKATGRFSNGRIPTDFISEAFGLKP +PAYLDPAYNISDLATG+TFASAGTGYDN
Sbjct: 63  RDFAGGKATGRFSNGRIPTDFISEAFGLKPTIPAYLDPAYNISDLATGLTFASAGTGYDN 122

Query: 122 ATSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMP 181
           ATSNVLSVI LWKQLE YKEYQAKL AYQGS+TAN+TI EALYVMSLGTNDFLENYYTMP
Sbjct: 123 ATSNVLSVIPLWKQLEYYKEYQAKLTAYQGSSTANETIKEALYVMSLGTNDFLENYYTMP 182

Query: 182 GRSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCV 241
           GRSSQY+IQQYQDFLVGIA+GFIEKL+ LGARKISLGGLPPMGCLPLERT NVFGG DC+
Sbjct: 183 GRSSQYSIQQYQDFLVGIASGFIEKLYSLGARKISLGGLPPMGCLPLERTRNVFGGYDCL 242

Query: 242 ESYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCA 301
           ESYNN+AV+FN KLKALTVKLN DLP I LVFSNPY VL+ MIKKPSLYGFDVTSTACCA
Sbjct: 243 ESYNNVAVDFNNKLKALTVKLNRDLPGIQLVFSNPYDVLMSMIKKPSLYGFDVTSTACCA 302

Query: 302 TGMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           TGMFEMGYACNR SMFTC DANKYIFWDSFHPTQKTNQ+VS+YVVKNVLSQFL
Sbjct: 303 TGMFEMGYACNRESMFTCTDANKYIFWDSFHPTQKTNQLVSSYVVKNVLSQFL 355

BLAST of Lag0015195 vs. ExPASy TrEMBL
Match: A0A6J1ERN5 (GDSL esterase/lipase At2g04570-like OS=Cucurbita moschata OX=3662 GN=LOC111437175 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.2e-174
Identity = 310/354 (87.57%), Postives = 329/354 (92.94%), Query Frame = 0

Query: 1   MAY-MFFLCLLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYG 60
           MAY +F LCLL      +SSP TI EA++S+IIVFGDSSVDAGNNNFIPTIARSNF PYG
Sbjct: 1   MAYIVFVLCLL----SFLSSPNTIAEARISSIIVFGDSSVDAGNNNFIPTIARSNFFPYG 60

Query: 61  RDFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDN 120
           RDFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISD AT VTFASAGTGYDN
Sbjct: 61  RDFAGGKATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDFATRVTFASAGTGYDN 120

Query: 121 ATSNVLSVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMP 180
           ATS+VLSVISLWKQLE YKEYQ KLRAYQGSATANQTINEALY+MSLGTNDFLENYYTMP
Sbjct: 121 ATSDVLSVISLWKQLEYYKEYQTKLRAYQGSATANQTINEALYLMSLGTNDFLENYYTMP 180

Query: 181 GRSSQYNIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVF-GGNDC 240
           GRSS YNIQQYQDFL+GIA GFIEKLHGLGARKISLGGLPPMGCLPLERT NVF G +DC
Sbjct: 181 GRSSHYNIQQYQDFLIGIAGGFIEKLHGLGARKISLGGLPPMGCLPLERTRNVFRGKDDC 240

Query: 241 VESYNNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACC 300
           VESYNN+AVEFN KL ALTVKLN +LPE++LVFSNPY VL+YMIKKPSLYGFDVTSTACC
Sbjct: 241 VESYNNVAVEFNNKLNALTVKLNKELPELELVFSNPYNVLMYMIKKPSLYGFDVTSTACC 300

Query: 301 ATGMFEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           ATGMFEMGYACNRN+MFTC +ANKYIFWDSFHPTQKTNQIV+AY+VKNVLS+FL
Sbjct: 301 ATGMFEMGYACNRNNMFTCTNANKYIFWDSFHPTQKTNQIVAAYMVKNVLSKFL 350

BLAST of Lag0015195 vs. TAIR 10
Match: AT2G04570.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 478.8 bits (1231), Expect = 3.8e-135
Identity = 226/338 (66.86%), Postives = 271/338 (80.18%), Query Frame = 0

Query: 16  LISSPKTIVEA-KVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGGKATGRFSNG 75
           LI+   T+  A K+ AIIVFGDSSVDAGNNN+IPT+ARSNF PYGRDF GGK TGRF NG
Sbjct: 13  LIAMSSTVTFAGKIPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNG 72

Query: 76  RIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVLSVISLWKQL 135
           +I TDF+SEA GLKPI+PAYLDP+YNISD ATGVTFASA TGYDNATS+VLSV+ LWKQL
Sbjct: 73  KIATDFMSEALGLKPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQL 132

Query: 136 ENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQYNIQQYQDFL 195
           E YKEYQ KL+AYQG     +TI  +LY++S+GTNDFLENY+  PGRSSQY++  YQDFL
Sbjct: 133 EYYKEYQTKLKAYQGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFL 192

Query: 196 VGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYNNLAVEFNKKLK 255
            GIA  F++KLHGLGARKISLGGLPPMGC+PLER TN+  G +CV  YN++AV+FN KL 
Sbjct: 193 AGIAKEFVKKLHGLGARKISLGGLPPMGCMPLERATNIGTGGECVGRYNDIAVQFNSKLD 252

Query: 256 ALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMFEMGYACNRNSM 315
            +  KL+ +LP  +LVFSNPY   + +IK PS +GF+V   ACCATGMFEMGY C RN+ 
Sbjct: 253 KMVEKLSKELPGSNLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNP 312

Query: 316 FTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
           FTC +A+KY+FWDSFHPTQKTN I++  ++ +    FL
Sbjct: 313 FTCTNADKYVFWDSFHPTQKTNHIMANALMNSTFPHFL 350

BLAST of Lag0015195 vs. TAIR 10
Match: AT2G42990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 432.6 bits (1111), Expect = 3.1e-121
Identity = 200/337 (59.35%), Postives = 267/337 (79.23%), Query Frame = 0

Query: 15  LLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGGKATGRFSNG 74
           +++++  +I  AK+ AIIVFGDSSVD+GNNNFI T+AR+NF PYGRDF GG+ATGRF NG
Sbjct: 13  IILTTLVSIAGAKIPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNG 72

Query: 75  RIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVLSVISLWKQL 134
           R+ +DF SEA+GLKP VPAYLDP+YNISD ATGV FASAGTGYDN+T++VL VI LWK++
Sbjct: 73  RLSSDFTSEAYGLKPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEV 132

Query: 135 ENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQYNIQQYQDFL 194
           E +KEYQ+ L AY G   A + I E+LY++S+GTNDFLENYYT+P R SQ++I QYQDFL
Sbjct: 133 EYFKEYQSNLSAYLGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFL 192

Query: 195 VGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYNNLAVEFNKKLK 254
           V IA  F++ ++ LGARK+S  G+ PMGCLPLER TN+     C  SYN+LAV+FN +L+
Sbjct: 193 VEIAEVFLKDIYRLGARKMSFTGISPMGCLPLERVTNLDDPFSCARSYNDLAVDFNGRLR 252

Query: 255 ALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMFEMGYACNRNSM 314
            L  KLN +L  I + F+NPY ++  ++ KP+LYG +++S+ACC TG+FEMG+ C +++ 
Sbjct: 253 RLVTKLNRELTGIKIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNP 312

Query: 315 FTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQF 352
            TC DANK++FWD+FHPT++TNQIVS +  K++ + F
Sbjct: 313 LTCSDANKFVFWDAFHPTERTNQIVSDHFFKHLKNLF 349

BLAST of Lag0015195 vs. TAIR 10
Match: AT4G26790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 4.2e-118
Identity = 205/343 (59.77%), Postives = 262/343 (76.38%), Query Frame = 0

Query: 9   LLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGGKAT 68
           LL+AA LL+  P+T   AK  A+IVFGDS+VD+GNNN I T+ +SNF PYGRD+  GKAT
Sbjct: 10  LLLAAQLLVKIPETC--AKFPALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKAT 69

Query: 69  GRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVLSVI 128
           GRFSNGRI  DFISE  GLK  VPAYLDPAYNI+D ATGV FASAGTG DNATS VLSV+
Sbjct: 70  GRFSNGRIAPDFISEGLGLKNAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVLSVM 129

Query: 129 SLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQYNIQ 188
            LWK++E YKEYQ +LR+Y G   AN+ I+E+LY++S+GTNDFLENYY +P +  +Y++ 
Sbjct: 130 PLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKYSVN 189

Query: 189 QYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYNNLAVE 248
           +YQ FL+GIAA F+  ++ LGARK+SL GL P GCLPLERTT +F G+ C+E YN +A +
Sbjct: 190 EYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQLFYGSKCIEEYNIVARD 249

Query: 249 FNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMFEMGYA 308
           FN K++    +LN DL  I LVFSNPY ++  +I  P  +GF+   +ACC TG +EM Y 
Sbjct: 250 FNIKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENVRSACCGTGYYEMSYL 309

Query: 309 CNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQF 352
           C++ + FTC DA+KY+FWDSFHPT+KTN IV+ +V+K  LS+F
Sbjct: 310 CDKMNPFTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRF 350

BLAST of Lag0015195 vs. TAIR 10
Match: AT4G26790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 4.2e-118
Identity = 205/343 (59.77%), Postives = 262/343 (76.38%), Query Frame = 0

Query: 9   LLIAAHLLISSPKTIVEAKVSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGGKAT 68
           LL+AA LL+  P+T   AK  A+IVFGDS+VD+GNNN I T+ +SNF PYGRD+  GKAT
Sbjct: 10  LLLAAQLLVKIPETC--AKFPALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKAT 69

Query: 69  GRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVLSVI 128
           GRFSNGRI  DFISE  GLK  VPAYLDPAYNI+D ATGV FASAGTG DNATS VLSV+
Sbjct: 70  GRFSNGRIAPDFISEGLGLKNAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVLSVM 129

Query: 129 SLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQYNIQ 188
            LWK++E YKEYQ +LR+Y G   AN+ I+E+LY++S+GTNDFLENYY +P +  +Y++ 
Sbjct: 130 PLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKYSVN 189

Query: 189 QYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGGNDCVESYNNLAVE 248
           +YQ FL+GIAA F+  ++ LGARK+SL GL P GCLPLERTT +F G+ C+E YN +A +
Sbjct: 190 EYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQLFYGSKCIEEYNIVARD 249

Query: 249 FNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGMFEMGYA 308
           FN K++    +LN DL  I LVFSNPY ++  +I  P  +GF+   +ACC TG +EM Y 
Sbjct: 250 FNIKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENVRSACCGTGYYEMSYL 309

Query: 309 CNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQF 352
           C++ + FTC DA+KY+FWDSFHPT+KTN IV+ +V+K  LS+F
Sbjct: 310 CDKMNPFTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRF 350

BLAST of Lag0015195 vs. TAIR 10
Match: AT1G59406.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.8e-74
Identity = 142/350 (40.57%), Postives = 212/350 (60.57%), Query Frame = 0

Query: 9   LLIAAHLLISSPKTIVEAK---VSAIIVFGDSSVDAGNNNFIPTIARSNFLPYGRDFAGG 68
           LL A  L+        + K   + A+IVFGDS +D GNNN +PT+ + NF PYG+D+ GG
Sbjct: 6   LLFALVLIFVEANAATQGKNTTIPALIVFGDSIMDTGNNNNLPTLLKCNFPPYGKDYPGG 65

Query: 69  KATGRFSNGRIPTDFISEAFGLKPIVPAYLDPAYNISDLATGVTFASAGTGYDNATSNVL 128
            ATGRFS+GR+P+D I+E  GL   +PAY++P     DL  GVTFAS GTGYD  T+ ++
Sbjct: 66  FATGRFSDGRVPSDLIAEKLGLAKTLPAYMNPYLKPEDLLKGVTFASGGTGYDPLTAKIM 125

Query: 129 SVISLWKQLENYKEYQAKLRAYQGSATANQTINEALYVMSLGTNDFLENYYTMPGRSSQY 188
           SVIS+W QL N+KEY +K++ + G   A   +  + +++   +ND    Y     ++ +Y
Sbjct: 126 SVISVWDQLINFKEYISKIKRHFGEEKAKDILEHSFFLVVSSSNDLAHTYL---AQTHRY 185

Query: 189 NIQQYQDFLVGIAAGFIEKLHGLGARKISLGGLPPMGCLPLERTTNVFGG---NDCVESY 248
           +   Y +FL   A  F+ +LH LGARKI +    P+GC+PL+RT  VFGG     C +  
Sbjct: 186 DRTSYANFLADSAVHFVRELHKLGARKIGVFSAVPVGCVPLQRT--VFGGFFTRGCNQPL 245

Query: 249 NNLAVEFNKKLKALTVKLNNDLPEIDLVFSNPYYVLLYMIKKPSLYGFDVTSTACCATGM 308
           NN+A +FN +L      L+ +L  + +++ N Y  L  MI+ P  YGF+V    CC  G+
Sbjct: 246 NNMAKQFNARLSPALDSLDKELDGV-ILYINVYDTLFDMIQHPKKYGFEVADRGCCGKGL 305

Query: 309 FEMGYACNRNSMFTCKDANKYIFWDSFHPTQKTNQIVSAYVVKNVLSQFL 353
             + Y CN  + FTC +++ YIFWDS+HP+++  Q+    +V N+L ++L
Sbjct: 306 LAISYLCNSLNPFTCSNSSAYIFWDSYHPSERAYQV----IVDNLLDKYL 345