Lag0015185 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0015185 vs. NCBI nr
Match: XP_038887470.1 (clathrin coat assembly protein AP180 [Benincasa hispida])

HSP 1 Score: 946.8 bits (2446), Expect = 8.7e-272
Identity = 494/603 (81.92%), Postives = 527/603 (87.40%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSIGLAKVS+SA GG NA+SLEVA+LKATTHDD PLDHRYVSEIL
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSA-GGPNATSLEVAVLKATTHDDGPLDHRYVSEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKS+AAACAHSIAKRITKTRNWIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  QLISANKSNAAACAHSIAKRITKTRNWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRK E++ GT RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKIESYHGTTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           +DGLALLLDSFFHLQYQSCVNAFQACVKA KQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 TDGLALLLDSFFHLQYQSCVNAFQACVKATKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSDKNSERGSDFN 360
           SDELIETLQEFLKDQASFPCHG+RSPP Q IL GP  KL DDLEQSESSDKNSERGSD N
Sbjct: 301 SDELIETLQEFLKDQASFPCHGSRSPPPQAILPGPTTKLTDDLEQSESSDKNSERGSDLN 360

Query: 361 SYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGPKP 420
           S GDIMSATGSWTSPANSVEQDGE +      +QS+LGDF NES LSPNFAFFEENG  P
Sbjct: 361 SVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENG-TP 420

Query: 421 FQEESQLGGAAPSFRFGDWELVLAESAT-EPQEWPDFFSPSVGDDLFVKPFSPEHHNNNP 480
           FQE     GA PS RFGDWELVLAESAT  PQEWPDFFSPS+GDDLF KPFSP+HH NNP
Sbjct: 421 FQEPQ--FGATPSVRFGDWELVLAESATGSPQEWPDFFSPSIGDDLFAKPFSPQHHYNNP 480

Query: 481 FLQDSKDLFTVPPTFKAESEIAPTFRAQ----SFGIAPTFRAETATVGSVNENGSLFEDW 540
           FLQDS DL  VPPTFKAE EIAPTFRAQ    +  +APTFRAETAT+ S N+N S+FE W
Sbjct: 481 FLQDSDDLLAVPPTFKAEREIAPTFRAQNSKETVSVAPTFRAETATLSSKNQNSSMFEAW 540

Query: 541 GSKRSKESNVFGF-DMGIENDPFAA-------GDVMN--EESLLQQQKLWKEQQSKIIAK 582
           G+ ++     FGF D+G+ENDPFA        G+  N  +ESL+QQQKLWKEQQ+KIIAK
Sbjct: 541 GTVQNGRG--FGFDDLGVENDPFAGPWSRNLNGNDPNFDQESLMQQQKLWKEQQNKIIAK 597

BLAST of Lag0015185 vs. NCBI nr
Match: XP_008449479.1 (PREDICTED: clathrin coat assembly protein AP180 [Cucumis melo] >KAA0061650.1 clathrin coat assembly protein AP180 [Cucumis melo var. makuwa] >TYK05856.1 clathrin coat assembly protein AP180 [Cucumis melo var. makuwa])

HSP 1 Score: 933.7 bits (2412), Expect = 7.6e-268
Identity = 488/595 (82.02%), Postives = 517/595 (86.89%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSIGLAKVS+SA GG NA+SLEVA+LKATTHDDVPLDHRYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSA-GGPNATSLEVAVLKATTHDDVPLDHRYVNEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKSHAAACAHSIAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  TLISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTE++  T RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSDKNSERGSDFN 360
           SDELIETLQEFLKDQASFPCHGNRSPP Q IL  P  KL DDLEQSESSDKNSERGSDFN
Sbjct: 301 SDELIETLQEFLKDQASFPCHGNRSPP-QAILSVPMTKLTDDLEQSESSDKNSERGSDFN 360

Query: 361 SYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGPKP 420
           S GDIMSATGSWTSPANSVEQDGE +      +QS+LGDF NES LSPNFAFFEENG  P
Sbjct: 361 SVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENG-MP 420

Query: 421 FQEESQLGGAAPSFRFGDWELVLAESAT-EPQEWPDFFSPSVGDDLFVKPFSPEHHNNNP 480
           F+EESQ  G APS RFGDWELVLAESAT  P+EWPDFFSPS+GDDLF KPFSP+HH  NP
Sbjct: 421 FEEESQF-GVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFSPQHHYKNP 480

Query: 481 FLQDSKDLFTVPPTFKAESEIAPTFRAQSFGIAPTFRAETATVGSVNENGSLFEDWGSKR 540
           FLQDS DLFT PPTFKAE E+ PTFRA++  +APTFRAETAT+ S NEN           
Sbjct: 481 FLQDSDDLFTAPPTFKAEKEMGPTFRAEN-SVAPTFRAETATLSSQNENR---------- 540

Query: 541 SKESNVFGFD-MGIENDPFAA-----GDVMNEESLLQQQKLWKEQQSKIIAKNSV 582
                  GFD +G  NDPFA          +EESLLQQQKLWKEQQ+KIIAK+++
Sbjct: 541 -------GFDELGFGNDPFAGPLNGNDQSFDEESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of Lag0015185 vs. NCBI nr
Match: XP_004140244.1 (clathrin coat assembly protein AP180 [Cucumis sativus] >KGN47964.1 hypothetical protein Csa_003702 [Cucumis sativus])

HSP 1 Score: 913.7 bits (2360), Expect = 8.1e-262
Identity = 477/595 (80.17%), Postives = 513/595 (86.22%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSIGLAKVS+SA GG NA+SLEV +LKATTHDD PLDHRYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSA-GGPNATSLEVVVLKATTHDDSPLDHRYVTEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKS+AAACAHSIAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  TLISANKSNAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRK E++  T RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKAESYHATTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSDKNSERGSDFN 360
           SDELIETLQEFLKDQASFPCHGNRSPP Q IL G   K  DDL+QSESSDKNSERGSDFN
Sbjct: 301 SDELIETLQEFLKDQASFPCHGNRSPP-QPILPGSITKFTDDLDQSESSDKNSERGSDFN 360

Query: 361 SYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGPKP 420
           S GDIMSATGSWTSPANSVEQDGE +      +QS+LGDF NE  LSPNFAFFEE+G   
Sbjct: 361 SVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANELNLSPNFAFFEESG-MA 420

Query: 421 FQEESQLGGAAPSFRFGDWELVLAESATE-PQEWPDFFSPSVGDDLFVKPFSPEHHNNNP 480
           F+EESQ  G APS RFGDWELVLAESAT+ P+EWPDFFSPS+GD+L+ KPFSP+HH  NP
Sbjct: 421 FEEESQF-GVAPSMRFGDWELVLAESATDSPKEWPDFFSPSIGDELYAKPFSPQHHYKNP 480

Query: 481 FLQDSKDLFTVPPTFKAESEIAPTFRAQSFGIAPTFRAETATVGSVNENGSLFEDWGSKR 540
           FLQDS DLFT PPTFKAE E+ PTFRA++  +APTFRAETAT+ S NEN           
Sbjct: 481 FLQDSDDLFTAPPTFKAEKEMGPTFRAEN-SVAPTFRAETATLSSQNEN----------- 540

Query: 541 SKESNVFGFD-MGIENDPFAA-----GDVMNEESLLQQQKLWKEQQSKIIAKNSV 582
                  GFD +G  NDPFA        + +EESLLQQQKLWKEQQ+KIIAK+++
Sbjct: 541 ------CGFDELGFGNDPFAGPLNGNDPIFDEESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of Lag0015185 vs. NCBI nr
Match: XP_023553928.1 (clathrin coat assembly protein AP180-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.0 bits (2335), Expect = 6.5e-259
Identity = 472/607 (77.76%), Postives = 517/607 (85.17%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSI LAKVS+S  GG NA SLEVAILKATTHDDVPL+ RYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSST-GGPNAISLEVAILKATTHDDVPLEQRYVAEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKSHAA CAHSIAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  QLISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT+RKTE+F G  RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHGNTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLV +SLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGG--PPAKLLDDLEQSESSDKNSERGSD 360
           SDELIETLQEFLKDQASFPCH +RSPP+ VIL G     KL DDLEQSESSDKNSER  D
Sbjct: 301 SDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELD 360

Query: 361 FNSYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGP 420
           FNS+G++MSATGSWTSPANSVEQDGE +      +QS+LGDF NES LSPNFAFF+++  
Sbjct: 361 FNSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFDDD-E 420

Query: 421 KPFQEESQLGGAAPSFRFGDWELVLAESATE-PQEWPDFFSPSVGDDLFVKPF-SPEHHN 480
            PFQEES+  GA P  R GDWELVLAESAT+  +EWPDFF+PS+GDD F KPF SP+H  
Sbjct: 421 TPFQEESEF-GATPGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFTSPQHQY 480

Query: 481 NNPFLQDSKDLFTVPPTFKAESEIAPTFRAQSFG----IAPTFRAETATVGSVNENGSLF 540
           NNPFLQDS + F VPPTFKAE+EIAPTFRA++      +APTFR ETAT  S N+NGS+F
Sbjct: 481 NNPFLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVVVAPTFRVETATFSSQNQNGSMF 540

Query: 541 EDWGSKRSKESNVFGFDMGIENDPFAAG------------DVMNEESLLQQQKLWKEQQS 581
           +DWG   +   + F FD+G ENDPFA              +VM++ESLLQQQKLWKE Q+
Sbjct: 541 DDWGPIPNGSGSGFEFDLGAENDPFAEAWDSNLNGNEPSLNVMDQESLLQQQKLWKEHQN 600

BLAST of Lag0015185 vs. NCBI nr
Match: XP_022952666.1 (clathrin coat assembly protein AP180 [Cucurbita moschata])

HSP 1 Score: 902.9 bits (2332), Expect = 1.4e-258
Identity = 472/607 (77.76%), Postives = 518/607 (85.34%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSI LAKVS+S  GG NA SLEVAILKATTHDDVPL+ RYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSST-GGPNAISLEVAILKATTHDDVPLEQRYVAEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKSHAA CAHSIAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  QLISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT+RKTE+F G  RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHGNTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLV +SLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGG--PPAKLLDDLEQSESSDKNSERGSD 360
           SDELIETLQEFLKDQASFPCH +RSPP+ VIL G     KL DDLEQSESSDKNSER  D
Sbjct: 301 SDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELD 360

Query: 361 FNSYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGP 420
           FNS+G++MSATGSWTSPANSVEQ+GE +      +QS+LGDF NES LSPNFAFFE++  
Sbjct: 361 FNSFGEMMSATGSWTSPANSVEQEGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEDD-E 420

Query: 421 KPFQEESQLGGAAPSFRFGDWELVLAESATE-PQEWPDFFSPSVGDDLFVKPF-SPEHHN 480
            PFQEE++L GAA   R GDWELVLAESAT+  +EWPDFF+PS+GDD F KPF SP+H  
Sbjct: 421 TPFQEETEL-GAARGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFTSPQHQY 480

Query: 481 NNPFLQDSKDLFTVPPTFKAESEIAPTFRA----QSFGIAPTFRAETATVGSVNENGSLF 540
           NNPFLQDS + F VPPTFKAE+EIAPTFRA    ++  +APTFR ET T  S N+NGS+F
Sbjct: 481 NNPFLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVAVAPTFRVETPTFSSQNQNGSMF 540

Query: 541 EDWGSKRSKESNVFGFDMGIENDPFAAG------------DVMNEESLLQQQKLWKEQQS 581
           +DWG   +   + F FD+G ENDPFA              +VM++ESLLQQQKLWKEQQ+
Sbjct: 541 DDWGPIPNGSGSGFEFDLGAENDPFAEAWDSSLNGNEPSLNVMDQESLLQQQKLWKEQQN 600

BLAST of Lag0015185 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.6e-143
Identity = 324/668 (48.50%), Postives = 403/668 (60.33%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHD-DVPLDHRYVSEI 60
           MPSK +KAI AVKDQTSI LAKV+  A GG + ++LEVAILKAT+HD +VP+D R V+EI
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEI 60

Query: 61  LLLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRG 120
           L +ISS KSHAA+CA +I +RI +TRNWIVALKSL LVLRIFQDGDPYFPREVLHAMKRG
Sbjct: 61  LGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRG 120

Query: 121 AKILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRK-----TENF 180
           AKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFLTGKLQ+R+T R+     + N 
Sbjct: 121 AKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNS 180

Query: 181 GTARRFN---------EAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSL 240
            T  RFN          A+RDMKP +L+D+I YWQKLLDRAIATRPTG AK NRLV+ SL
Sbjct: 181 TTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSL 240

Query: 241 HAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSI 300
           +AV+QESFDLYRDISDGLALLLDSFFHLQYQSC+NAFQACV+A+KQFEEL +FYDL KSI
Sbjct: 241 YAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSI 300

Query: 301 GVGRTSEYPSIQQPSDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAK----LLDD 360
           G+GRTSEYPSIQ+ S EL+ETLQEFLKDQ+SFP      P     L  PP+     +   
Sbjct: 301 GIGRTSEYPSIQKISLELLETLQEFLKDQSSFPASSGLYPSPNSFLPPPPSSKDSAVSSS 360

Query: 361 LEQSESSDKNSERGSDFNSY-----GDIMSATGSWT-SPANSV----------EQDGESF 420
           L+  +S+   SER SD+ S+      D+MS T + T SP  S           + +G +F
Sbjct: 361 LDFGDSTIDTSERYSDYGSFRSTSLEDLMSRTEAGTSSPPMSCHSEPYGGGRDDPNGNNF 420

Query: 421 EQSQLGDFVNESILS--------------PNFAFFEENGPKPFQEESQLGGAAP------ 480
           +        N   +S               N A  E+   K  Q++S+     P      
Sbjct: 421 DTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKAETFDPWEALML 480

Query: 481 ---------------------SFRFGDWELVLAESATEPQEWPDFFSPSVGDDLFVKPFS 540
                                    G+W L L E+AT+ Q          G D  +  F 
Sbjct: 481 RDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQVQGNNSMAIVPFGLDDPMPAFQ 540

Query: 541 PEHHNNNPFLQD--------SKDLFTVPPTFKAESEIAPTFRAQ-----SFGIAPTFRAE 580
                 NPFL++         + + T         +  PTF+            PTF+A 
Sbjct: 541 AATDQYNPFLEEPVAQLATAGEPMITFGGLALTGFQPEPTFQVNVPDDFEPSSTPTFKA- 600

BLAST of Lag0015185 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 274.2 bits (700), Expect = 3.3e-72
Identity = 156/383 (40.73%), Postives = 228/383 (59.53%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           M    RKAI  VKDQTSIG+AKV+++      A  LEVAI+KAT+HDD     +Y+ EIL
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASNM-----APDLEVAIVKATSHDDDQSSDKYIREIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            L S ++ +  AC  S+++R+ KTR+WIVALK+L LV R+  +GDP F  E+L+A +RG 
Sbjct: 61  SLTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGT 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQK---------------- 180
           +ILN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L  +  +                
Sbjct: 121 RILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDG 180

Query: 181 --------RFTQRKTENFGTARRF------------NE-----------AIRDMKPAVLI 240
                   R    +T ++ T   F            NE            +R+M P  + 
Sbjct: 181 YNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIF 240

Query: 241 DRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDISDGLALLLDSFFHL 300
            ++ + Q+LLDR ++ RPTG AK +R++  +++ VV+ESF LY DI + LA+LLD FF +
Sbjct: 241 GKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDM 300

Query: 301 QYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQPSDELIETLQEFLKD 337
           +Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP +Q+ + +L+ETL+EF++D
Sbjct: 301 EYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRD 360

BLAST of Lag0015185 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 3.6e-71
Identity = 154/402 (38.31%), Postives = 234/402 (58.21%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           M    RKAI AVKDQTSIG+AKV+++      A  LEVAI+KAT+HDD P   +Y+ EIL
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASNM-----APDLEVAIVKATSHDDDPASEKYIREIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            L S ++ +  AC  S+++R++KTR+W+VALK+L LV R+  +GDP F  E+L++ +RG 
Sbjct: 61  NLTSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGT 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFL---------------------- 180
           ++LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L                      
Sbjct: 121 RMLNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNND 180

Query: 181 --TGKLQKRF-----------------------------TQRKTENFG------------ 240
              G+ +  F                               +++ ++G            
Sbjct: 181 DRYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGG 240

Query: 241 --TARRFNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESF 300
               ++    +R+M P  +  ++ + Q+LLDR ++ RPTG AK +R++  +L+ VV+ESF
Sbjct: 241 GRDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESF 300

Query: 301 DLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEY 336
            LY DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEY
Sbjct: 301 KLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEY 360

BLAST of Lag0015185 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-68
Identity = 159/473 (33.62%), Postives = 260/473 (54.97%), Query Frame = 0

Query: 3   SKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEILLL 62
           SKF++AI AVKDQTS+GLAKV+   G  A+ S L+VAI+KAT H++ P + +Y+ EIL L
Sbjct: 4   SKFKRAIGAVKDQTSVGLAKVN---GRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 63

Query: 63  ISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 122
            S ++S+  AC  ++++R+ KT+ W VALK+L L+ R+  +GD  + +E+  A +RG ++
Sbjct: 64  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 123

Query: 123 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT------------QRK 182
           LN+S+FRD S S+ WDY+AFVRT+ALYLDERLD  +  +  KR               + 
Sbjct: 124 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQA 183

Query: 183 TENFGTARRF-NEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVV 242
             +  TA    ++ I +MK   +  RI + Q+LLDR +A RPTG A+ NR+V  +L+ +V
Sbjct: 184 AADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIV 243

Query: 243 QESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGR 302
           +ESF +Y D+++ + +L++ F  L     +  +    + +KQFEEL  FY  CK++G+ R
Sbjct: 244 KESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIAR 303

Query: 303 TSEYPSIQQPSDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSD 362
           +SEYP I++ + + ++ + EF++D+++   H  +S                 ++     D
Sbjct: 304 SSEYPEIEKITQKKLDLMDEFIRDKSALE-HTKQS---------------KSVKSEADED 363

Query: 363 KNSERGSDFNSYGDIMSATGSWTSPANSVEQDGESFE-----------QSQLGDFVN--- 422
            +  R  + N   + M+A  +   P    E D +  E           Q ++GD ++   
Sbjct: 364 DDEARTEEVNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGN 423

Query: 423 -----ESILSPNFAFFEENGPKPFQEESQLGGAAPSFR--FGDWELVLAESAT 442
                      + A    +GP      S+ G    +F+    DWE  L ++AT
Sbjct: 424 TNGGEAGQAGDSLALALFDGPYASGSGSESGPGWEAFKDDSADWETALVQTAT 457

BLAST of Lag0015185 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 261.9 bits (668), Expect = 1.7e-68
Identity = 157/476 (32.98%), Postives = 259/476 (54.41%), Query Frame = 0

Query: 3   SKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEILLL 62
           SK ++AI AVKDQTS+GLAKV    G  ++ + LE+A++KAT HDD P + +Y+ EIL L
Sbjct: 4   SKLKRAIGAVKDQTSVGLAKV---GGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 63

Query: 63  ISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 122
            S ++++ +AC  ++++R+ KT+NW VALK+L L+ R+  DGD  + +E+  A +RG ++
Sbjct: 64  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 123

Query: 123 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT--------------- 182
           LN+S+FRD S S  WDY+AFVRT+ALYLDERLD  + G+  K+ +               
Sbjct: 124 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDD 183

Query: 183 QRKTENFGTARRF---NEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHS 242
            R T N   ++     ++ + +MK   + +R+ + Q+LLDR +A RPTG AK NR+V  +
Sbjct: 184 HRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVA 243

Query: 243 LHAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKS 302
           ++ +V+ESF LY +I++ + +L++ F  L     +  ++   + +KQF+EL  FY  CK+
Sbjct: 244 MYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKN 303

Query: 303 IGVGRTSEYPSIQQPSDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQ 362
           + V R+SEYP +++ + + ++ + EF++D+++      +S           +K  +  E+
Sbjct: 304 MAVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKS----------SSKRSNKSEE 363

Query: 363 SESSDKNSERGSDFNSYGDIMSATGSWTSPANSVEQDGESFE-----------QSQLGDF 422
            ES  +  +   +     D+ S            E++ E  E           Q Q GD 
Sbjct: 364 EESKTEYIQENQE-----DLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDL 423

Query: 423 --------VNESILSPNFAFFEENGPKPFQEESQLGGAAPSFRFGDWELVLAESAT 442
                   V    +  + A    +G    +  S  G  A +    DWE  L  SAT
Sbjct: 424 LDLTDEAGVTAGTVGDSLALALFDGVVGTESASGPGWEAFNDNSADWETDLVRSAT 461

BLAST of Lag0015185 vs. ExPASy TrEMBL
Match: A0A5D3C685 (Clathrin coat assembly protein AP180 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00110 PE=4 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.7e-268
Identity = 488/595 (82.02%), Postives = 517/595 (86.89%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSIGLAKVS+SA GG NA+SLEVA+LKATTHDDVPLDHRYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSA-GGPNATSLEVAVLKATTHDDVPLDHRYVNEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKSHAAACAHSIAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  TLISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTE++  T RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSDKNSERGSDFN 360
           SDELIETLQEFLKDQASFPCHGNRSPP Q IL  P  KL DDLEQSESSDKNSERGSDFN
Sbjct: 301 SDELIETLQEFLKDQASFPCHGNRSPP-QAILSVPMTKLTDDLEQSESSDKNSERGSDFN 360

Query: 361 SYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGPKP 420
           S GDIMSATGSWTSPANSVEQDGE +      +QS+LGDF NES LSPNFAFFEENG  P
Sbjct: 361 SVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENG-MP 420

Query: 421 FQEESQLGGAAPSFRFGDWELVLAESAT-EPQEWPDFFSPSVGDDLFVKPFSPEHHNNNP 480
           F+EESQ  G APS RFGDWELVLAESAT  P+EWPDFFSPS+GDDLF KPFSP+HH  NP
Sbjct: 421 FEEESQF-GVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFSPQHHYKNP 480

Query: 481 FLQDSKDLFTVPPTFKAESEIAPTFRAQSFGIAPTFRAETATVGSVNENGSLFEDWGSKR 540
           FLQDS DLFT PPTFKAE E+ PTFRA++  +APTFRAETAT+ S NEN           
Sbjct: 481 FLQDSDDLFTAPPTFKAEKEMGPTFRAEN-SVAPTFRAETATLSSQNENR---------- 540

Query: 541 SKESNVFGFD-MGIENDPFAA-----GDVMNEESLLQQQKLWKEQQSKIIAKNSV 582
                  GFD +G  NDPFA          +EESLLQQQKLWKEQQ+KIIAK+++
Sbjct: 541 -------GFDELGFGNDPFAGPLNGNDQSFDEESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of Lag0015185 vs. ExPASy TrEMBL
Match: A0A1S3BN17 (clathrin coat assembly protein AP180 OS=Cucumis melo OX=3656 GN=LOC103491352 PE=4 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.7e-268
Identity = 488/595 (82.02%), Postives = 517/595 (86.89%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSIGLAKVS+SA GG NA+SLEVA+LKATTHDDVPLDHRYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSA-GGPNATSLEVAVLKATTHDDVPLDHRYVNEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKSHAAACAHSIAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  TLISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTE++  T RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSDKNSERGSDFN 360
           SDELIETLQEFLKDQASFPCHGNRSPP Q IL  P  KL DDLEQSESSDKNSERGSDFN
Sbjct: 301 SDELIETLQEFLKDQASFPCHGNRSPP-QAILSVPMTKLTDDLEQSESSDKNSERGSDFN 360

Query: 361 SYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGPKP 420
           S GDIMSATGSWTSPANSVEQDGE +      +QS+LGDF NES LSPNFAFFEENG  P
Sbjct: 361 SVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENG-MP 420

Query: 421 FQEESQLGGAAPSFRFGDWELVLAESAT-EPQEWPDFFSPSVGDDLFVKPFSPEHHNNNP 480
           F+EESQ  G APS RFGDWELVLAESAT  P+EWPDFFSPS+GDDLF KPFSP+HH  NP
Sbjct: 421 FEEESQF-GVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFSPQHHYKNP 480

Query: 481 FLQDSKDLFTVPPTFKAESEIAPTFRAQSFGIAPTFRAETATVGSVNENGSLFEDWGSKR 540
           FLQDS DLFT PPTFKAE E+ PTFRA++  +APTFRAETAT+ S NEN           
Sbjct: 481 FLQDSDDLFTAPPTFKAEKEMGPTFRAEN-SVAPTFRAETATLSSQNENR---------- 540

Query: 541 SKESNVFGFD-MGIENDPFAA-----GDVMNEESLLQQQKLWKEQQSKIIAKNSV 582
                  GFD +G  NDPFA          +EESLLQQQKLWKEQQ+KIIAK+++
Sbjct: 541 -------GFDELGFGNDPFAGPLNGNDQSFDEESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of Lag0015185 vs. ExPASy TrEMBL
Match: A0A0A0KGJ5 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421580 PE=4 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.9e-262
Identity = 477/595 (80.17%), Postives = 513/595 (86.22%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSIGLAKVS+SA GG NA+SLEV +LKATTHDD PLDHRYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSA-GGPNATSLEVVVLKATTHDDSPLDHRYVTEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKS+AAACAHSIAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  TLISANKSNAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRK E++  T RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKAESYHATTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSDKNSERGSDFN 360
           SDELIETLQEFLKDQASFPCHGNRSPP Q IL G   K  DDL+QSESSDKNSERGSDFN
Sbjct: 301 SDELIETLQEFLKDQASFPCHGNRSPP-QPILPGSITKFTDDLDQSESSDKNSERGSDFN 360

Query: 361 SYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGPKP 420
           S GDIMSATGSWTSPANSVEQDGE +      +QS+LGDF NE  LSPNFAFFEE+G   
Sbjct: 361 SVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANELNLSPNFAFFEESG-MA 420

Query: 421 FQEESQLGGAAPSFRFGDWELVLAESATE-PQEWPDFFSPSVGDDLFVKPFSPEHHNNNP 480
           F+EESQ  G APS RFGDWELVLAESAT+ P+EWPDFFSPS+GD+L+ KPFSP+HH  NP
Sbjct: 421 FEEESQF-GVAPSMRFGDWELVLAESATDSPKEWPDFFSPSIGDELYAKPFSPQHHYKNP 480

Query: 481 FLQDSKDLFTVPPTFKAESEIAPTFRAQSFGIAPTFRAETATVGSVNENGSLFEDWGSKR 540
           FLQDS DLFT PPTFKAE E+ PTFRA++  +APTFRAETAT+ S NEN           
Sbjct: 481 FLQDSDDLFTAPPTFKAEKEMGPTFRAEN-SVAPTFRAETATLSSQNEN----------- 540

Query: 541 SKESNVFGFD-MGIENDPFAA-----GDVMNEESLLQQQKLWKEQQSKIIAKNSV 582
                  GFD +G  NDPFA        + +EESLLQQQKLWKEQQ+KIIAK+++
Sbjct: 541 ------CGFDELGFGNDPFAGPLNGNDPIFDEESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of Lag0015185 vs. ExPASy TrEMBL
Match: A0A6J1I2N8 (clathrin coat assembly protein AP180-like OS=Cucurbita maxima OX=3661 GN=LOC111468453 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 7.0e-259
Identity = 475/609 (78.00%), Postives = 518/609 (85.06%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSI LAKVS+S  GG NA SLEVAILKATTHDDVPL+ RYV EIL
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSST-GGPNAISLEVAILKATTHDDVPLEQRYVVEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKSHAA CAHSIAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  HLISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT+RKTE+F G  RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFNGNTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLV +SLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGG--PPAKLLDDLEQSESSDKNSERGSD 360
           SDELIETLQEFLKDQASFPCH +RSPP+ VIL G     KL DDLEQSESSDKNSER  D
Sbjct: 301 SDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELD 360

Query: 361 FNSYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGP 420
           FNS+G++MSATGSWTSPANSVEQDGE +      +QS+LGDF NES LSPNFAFFE++  
Sbjct: 361 FNSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEDD-E 420

Query: 421 KPFQEESQLGGAAPSFRFGDWELVLAESATE-PQEWPDFFSPSVGDDLFVKPF-SPEHHN 480
           KPFQEES+  GAAP  R GDWELVLAESAT+  +EWPDFF+PS+GDD F KPF SP+H  
Sbjct: 421 KPFQEESEF-GAAPGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFSSPQHQY 480

Query: 481 NNPFLQDSKDLFTVPPTFKAESEIAPTFRAQSFG----IAPTFRAETATVGSVNENGSLF 540
           NNPFLQDS + F VPPTFKAE+EIAPTFRA++      +APTFR ET T  S ++NGS+F
Sbjct: 481 NNPFLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVVVAPTFRVETPTFSSQHQNGSMF 540

Query: 541 EDWG--SKRSKESNVFGFDMGIENDPFAAG------------DVMNEESLLQQQKLWKEQ 581
           +DWG     S   + F FD+G ENDPFA              +VM++ESLLQQQKLWKEQ
Sbjct: 541 DDWGPIPNGSGSGSGFEFDLGAENDPFAEAWNSNLNGNEPSLNVMDQESLLQQQKLWKEQ 600

BLAST of Lag0015185 vs. ExPASy TrEMBL
Match: A0A6J1GKV6 (clathrin coat assembly protein AP180 OS=Cucurbita moschata OX=3662 GN=LOC111455292 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 7.0e-259
Identity = 472/607 (77.76%), Postives = 518/607 (85.34%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           MPSK RKA+YAVKDQTSI LAKVS+S  GG NA SLEVAILKATTHDDVPL+ RYV+EIL
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSST-GGPNAISLEVAILKATTHDDVPLEQRYVAEIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            LIS+NKSHAA CAHSIAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGA
Sbjct: 61  QLISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGA 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTENF-GTARR 180
           KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT+RKTE+F G  RR
Sbjct: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHGNTRR 180

Query: 181 FNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDI 240
            NE IRDMKPA+LIDRI YWQKLLDRAIATRPTGPAKGNRLV +SLHAVVQESFDLYRDI
Sbjct: 181 INEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDI 240

Query: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQP 300
           SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPS+QQP
Sbjct: 241 SDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQP 300

Query: 301 SDELIETLQEFLKDQASFPCHGNRSPPSQVILGG--PPAKLLDDLEQSESSDKNSERGSD 360
           SDELIETLQEFLKDQASFPCH +RSPP+ VIL G     KL DDLEQSESSDKNSER  D
Sbjct: 301 SDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELD 360

Query: 361 FNSYGDIMSATGSWTSPANSVEQDGESF------EQSQLGDFVNESILSPNFAFFEENGP 420
           FNS+G++MSATGSWTSPANSVEQ+GE +      +QS+LGDF NES LSPNFAFFE++  
Sbjct: 361 FNSFGEMMSATGSWTSPANSVEQEGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEDD-E 420

Query: 421 KPFQEESQLGGAAPSFRFGDWELVLAESATE-PQEWPDFFSPSVGDDLFVKPF-SPEHHN 480
            PFQEE++L GAA   R GDWELVLAESAT+  +EWPDFF+PS+GDD F KPF SP+H  
Sbjct: 421 TPFQEETEL-GAARGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFTSPQHQY 480

Query: 481 NNPFLQDSKDLFTVPPTFKAESEIAPTFRA----QSFGIAPTFRAETATVGSVNENGSLF 540
           NNPFLQDS + F VPPTFKAE+EIAPTFRA    ++  +APTFR ET T  S N+NGS+F
Sbjct: 481 NNPFLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVAVAPTFRVETPTFSSQNQNGSMF 540

Query: 541 EDWGSKRSKESNVFGFDMGIENDPFAAG------------DVMNEESLLQQQKLWKEQQS 581
           +DWG   +   + F FD+G ENDPFA              +VM++ESLLQQQKLWKEQQ+
Sbjct: 541 DDWGPIPNGSGSGFEFDLGAENDPFAEAWDSSLNGNEPSLNVMDQESLLQQQKLWKEQQN 600

BLAST of Lag0015185 vs. TAIR 10
Match: AT1G05020.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.5e-144
Identity = 324/668 (48.50%), Postives = 403/668 (60.33%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHD-DVPLDHRYVSEI 60
           MPSK +KAI AVKDQTSI LAKV+  A GG + ++LEVAILKAT+HD +VP+D R V+EI
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEI 60

Query: 61  LLLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRG 120
           L +ISS KSHAA+CA +I +RI +TRNWIVALKSL LVLRIFQDGDPYFPREVLHAMKRG
Sbjct: 61  LGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRG 120

Query: 121 AKILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRK-----TENF 180
           AKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFLTGKLQ+R+T R+     + N 
Sbjct: 121 AKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNS 180

Query: 181 GTARRFN---------EAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSL 240
            T  RFN          A+RDMKP +L+D+I YWQKLLDRAIATRPTG AK NRLV+ SL
Sbjct: 181 TTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSL 240

Query: 241 HAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSI 300
           +AV+QESFDLYRDISDGLALLLDSFFHLQYQSC+NAFQACV+A+KQFEEL +FYDL KSI
Sbjct: 241 YAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSI 300

Query: 301 GVGRTSEYPSIQQPSDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAK----LLDD 360
           G+GRTSEYPSIQ+ S EL+ETLQEFLKDQ+SFP      P     L  PP+     +   
Sbjct: 301 GIGRTSEYPSIQKISLELLETLQEFLKDQSSFPASSGLYPSPNSFLPPPPSSKDSAVSSS 360

Query: 361 LEQSESSDKNSERGSDFNSY-----GDIMSATGSWT-SPANSV----------EQDGESF 420
           L+  +S+   SER SD+ S+      D+MS T + T SP  S           + +G +F
Sbjct: 361 LDFGDSTIDTSERYSDYGSFRSTSLEDLMSRTEAGTSSPPMSCHSEPYGGGRDDPNGNNF 420

Query: 421 EQSQLGDFVNESILS--------------PNFAFFEENGPKPFQEESQLGGAAP------ 480
           +        N   +S               N A  E+   K  Q++S+     P      
Sbjct: 421 DTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKAETFDPWEALML 480

Query: 481 ---------------------SFRFGDWELVLAESATEPQEWPDFFSPSVGDDLFVKPFS 540
                                    G+W L L E+AT+ Q          G D  +  F 
Sbjct: 481 RDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQVQGNNSMAIVPFGLDDPMPAFQ 540

Query: 541 PEHHNNNPFLQD--------SKDLFTVPPTFKAESEIAPTFRAQ-----SFGIAPTFRAE 580
                 NPFL++         + + T         +  PTF+            PTF+A 
Sbjct: 541 AATDQYNPFLEEPVAQLATAGEPMITFGGLALTGFQPEPTFQVNVPDDFEPSSTPTFKA- 600

BLAST of Lag0015185 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.3e-73
Identity = 156/383 (40.73%), Postives = 228/383 (59.53%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           M    RKAI  VKDQTSIG+AKV+++      A  LEVAI+KAT+HDD     +Y+ EIL
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASNM-----APDLEVAIVKATSHDDDQSSDKYIREIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            L S ++ +  AC  S+++R+ KTR+WIVALK+L LV R+  +GDP F  E+L+A +RG 
Sbjct: 61  SLTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGT 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQK---------------- 180
           +ILN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L  +  +                
Sbjct: 121 RILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDG 180

Query: 181 --------RFTQRKTENFGTARRF------------NE-----------AIRDMKPAVLI 240
                   R    +T ++ T   F            NE            +R+M P  + 
Sbjct: 181 YNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIF 240

Query: 241 DRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDISDGLALLLDSFFHL 300
            ++ + Q+LLDR ++ RPTG AK +R++  +++ VV+ESF LY DI + LA+LLD FF +
Sbjct: 241 GKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDM 300

Query: 301 QYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQPSDELIETLQEFLKD 337
           +Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP +Q+ + +L+ETL+EF++D
Sbjct: 301 EYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRD 360

BLAST of Lag0015185 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.3e-73
Identity = 156/383 (40.73%), Postives = 228/383 (59.53%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           M    RKAI  VKDQTSIG+AKV+++      A  LEVAI+KAT+HDD     +Y+ EIL
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASNM-----APDLEVAIVKATSHDDDQSSDKYIREIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            L S ++ +  AC  S+++R+ KTR+WIVALK+L LV R+  +GDP F  E+L+A +RG 
Sbjct: 61  SLTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGT 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQK---------------- 180
           +ILN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L  +  +                
Sbjct: 121 RILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDG 180

Query: 181 --------RFTQRKTENFGTARRF------------NE-----------AIRDMKPAVLI 240
                   R    +T ++ T   F            NE            +R+M P  + 
Sbjct: 181 YNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIF 240

Query: 241 DRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDISDGLALLLDSFFHL 300
            ++ + Q+LLDR ++ RPTG AK +R++  +++ VV+ESF LY DI + LA+LLD FF +
Sbjct: 241 GKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDM 300

Query: 301 QYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSIQQPSDELIETLQEFLKD 337
           +Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP +Q+ + +L+ETL+EF++D
Sbjct: 301 EYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRD 360

BLAST of Lag0015185 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 270.8 bits (691), Expect = 2.6e-72
Identity = 154/402 (38.31%), Postives = 234/402 (58.21%), Query Frame = 0

Query: 1   MPSKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEIL 60
           M    RKAI AVKDQTSIG+AKV+++      A  LEVAI+KAT+HDD P   +Y+ EIL
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASNM-----APDLEVAIVKATSHDDDPASEKYIREIL 60

Query: 61  LLISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGA 120
            L S ++ +  AC  S+++R++KTR+W+VALK+L LV R+  +GDP F  E+L++ +RG 
Sbjct: 61  NLTSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGT 120

Query: 121 KILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFL---------------------- 180
           ++LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L                      
Sbjct: 121 RMLNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNND 180

Query: 181 --TGKLQKRF-----------------------------TQRKTENFG------------ 240
              G+ +  F                               +++ ++G            
Sbjct: 181 DRYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGG 240

Query: 241 --TARRFNEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESF 300
               ++    +R+M P  +  ++ + Q+LLDR ++ RPTG AK +R++  +L+ VV+ESF
Sbjct: 241 GRDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESF 300

Query: 301 DLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEY 336
            LY DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEY
Sbjct: 301 KLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEY 360

BLAST of Lag0015185 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 9.2e-70
Identity = 159/473 (33.62%), Postives = 260/473 (54.97%), Query Frame = 0

Query: 3   SKFRKAIYAVKDQTSIGLAKVSASAGGGANASSLEVAILKATTHDDVPLDHRYVSEILLL 62
           SKF++AI AVKDQTS+GLAKV+   G  A+ S L+VAI+KAT H++ P + +Y+ EIL L
Sbjct: 4   SKFKRAIGAVKDQTSVGLAKVN---GRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 63

Query: 63  ISSNKSHAAACAHSIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 122
            S ++S+  AC  ++++R+ KT+ W VALK+L L+ R+  +GD  + +E+  A +RG ++
Sbjct: 64  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 123

Query: 123 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT------------QRK 182
           LN+S+FRD S S+ WDY+AFVRT+ALYLDERLD  +  +  KR               + 
Sbjct: 124 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQA 183

Query: 183 TENFGTARRF-NEAIRDMKPAVLIDRICYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVV 242
             +  TA    ++ I +MK   +  RI + Q+LLDR +A RPTG A+ NR+V  +L+ +V
Sbjct: 184 AADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIV 243

Query: 243 QESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGR 302
           +ESF +Y D+++ + +L++ F  L     +  +    + +KQFEEL  FY  CK++G+ R
Sbjct: 244 KESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIAR 303

Query: 303 TSEYPSIQQPSDELIETLQEFLKDQASFPCHGNRSPPSQVILGGPPAKLLDDLEQSESSD 362
           +SEYP I++ + + ++ + EF++D+++   H  +S                 ++     D
Sbjct: 304 SSEYPEIEKITQKKLDLMDEFIRDKSALE-HTKQS---------------KSVKSEADED 363

Query: 363 KNSERGSDFNSYGDIMSATGSWTSPANSVEQDGESFE-----------QSQLGDFVN--- 422
            +  R  + N   + M+A  +   P    E D +  E           Q ++GD ++   
Sbjct: 364 DDEARTEEVNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGN 423

Query: 423 -----ESILSPNFAFFEENGPKPFQEESQLGGAAPSFR--FGDWELVLAESAT 442
                      + A    +GP      S+ G    +F+    DWE  L ++AT
Sbjct: 424 TNGGEAGQAGDSLALALFDGPYASGSGSESGPGWEAFKDDSADWETALVQTAT 457