Lag0015175 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015175
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	PLASMODESMATA CALLOSE-BINDING PROTEIN 5
Location	chr12: 8345305 .. 8346003 (-)
RNA-Seq Expression	Lag0015175
Synteny	Lag0015175

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGATCCATTTCGCCCTCTCTCTCCTCACTCTCCTCATTCTCTGCACAGCGGCGGAGCCCCGGCAGCCGTCGATCAGAGACCAGAGCGGCTCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTACAATCCGCCCTCGACTGGGCCTGCGGTGCAGGCGGCGCCGACTGTTCCCCAATCCAGCCCGGCGGCGCCTGCTACGACGCCTCCGACGTCCAGAACATGGCCTCCTTCGCCTTCAACGACTACTTCCTCAAACACGGCATGACCGACGACAGTTGCTTCTTCCAAAACTCCGCCGCCATCACCTCCTTAAACCCTAGTCAGTTTCTGCAACTCCGATCTCTCAAATTTCTCCATCCAATCTTTAAATTCTTTCCAGATCTGTAACCGAATCGCTCATTGATTCTGATCTTTCTTGTTTCAGGCTACGGAAATTGCAGGTTCCCATCCAGGTAAGCTTCGTCGACGATCTTTAAAACCCCTGTGTTGGGATTTGGCATTTAGGGCATAATTAATGGCCGTAATCAAGAAATCAATCATCAACCACCACGAATCTGCAACTTCTTTTGCTTTTTTTTTCGGTTTTAATGGAATTGATTTCATTCATTTATGGCGCAGTTTGTTGAGAAATGGAAACTTTTCGAGCCAATCGACGTCGGTAGGGTTGGGGCCGAGTGA

mRNA sequence

ATGCCGATCCATTTCGCCCTCTCTCTCCTCACTCTCCTCATTCTCTGCACAGCGGCGGAGCCCCGGCAGCCGTCGATCAGAGACCAGAGCGGCTCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTACAATCCGCCCTCGACTGGGCCTGCGGTGCAGGCGGCGCCGACTGTTCCCCAATCCAGCCCGGCGGCGCCTGCTACGACGCCTCCGACGTCCAGAACATGGCCTCCTTCGCCTTCAACGACTACTTCCTCAAACACGGCATGACCGACGACAGTTGCTTCTTCCAAAACTCCGCCGCCATCACCTCCTTAAACCCTAGTCAGCTACGGAAATTGCAGGTTCCCATCCAGTTTGTTGAGAAATGGAAACTTTTCGAGCCAATCGACGTCGGTAGGGTTGGGGCCGAGTGA

Coding sequence (CDS)

ATGCCGATCCATTTCGCCCTCTCTCTCCTCACTCTCCTCATTCTCTGCACAGCGGCGGAGCCCCGGCAGCCGTCGATCAGAGACCAGAGCGGCTCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTACAATCCGCCCTCGACTGGGCCTGCGGTGCAGGCGGCGCCGACTGTTCCCCAATCCAGCCCGGCGGCGCCTGCTACGACGCCTCCGACGTCCAGAACATGGCCTCCTTCGCCTTCAACGACTACTTCCTCAAACACGGCATGACCGACGACAGTTGCTTCTTCCAAAACTCCGCCGCCATCACCTCCTTAAACCCTAGTCAGCTACGGAAATTGCAGGTTCCCATCCAGTTTGTTGAGAAATGGAAACTTTTCGAGCCAATCGACGTCGGTAGGGTTGGGGCCGAGTGA

Protein sequence

MPIHFALSLLTLLILCTAAEPRQPSIRDQSGSTELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSQLRKLQVPIQFVEKWKLFEPIDVGRVGAE

Homology

[Close]

BLAST of Lag0015175 vs. NCBI nr
Match: KAA0061642.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 [Cucumis melo var. makuwa] >TYK05864.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 5 [Cucumis melo var. makuwa])

HSP 1 Score: 251.5 bits (641), Expect = 4.3e-63
Identity = 123/145 (84.83%), Postives = 135/145 (93.10%), Query Frame = 0


Query: 1   MPIHFALSLLTLLILCTAAEP-RQPSIRDQSG-STELWCVAKNNADDASLQSALDWACGA 60
           MPI+F+L LL LLI CTAA+P R+PSIRDQSG +TELWCVAKNNADDASLQSALDWACGA
Sbjct: 1   MPINFSLFLLILLIPCTAADPWRRPSIRDQSGAATELWCVAKNNADDASLQSALDWACGA 60

Query: 61  GGADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSQLRK 120
           GGADCSPIQPGG+CYDA+DVQNMASFAFNDYF KHGMTDDSCFFQNSAAITSLNPS LRK
Sbjct: 61  GGADCSPIQPGGSCYDATDVQNMASFAFNDYFRKHGMTDDSCFFQNSAAITSLNPSMLRK 120

Query: 121 LQVPIQFVEKWKLFEPIDVGRVGAE 144
           LQ+PI+ VEKW+ F+P D+GRVGAE
Sbjct: 121 LQIPIKCVEKWEFFKPNDIGRVGAE 145

[Close]

BLAST of Lag0015175 vs. NCBI nr
Match: XP_038877601.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Benincasa hispida])

HSP 1 Score: 208.8 bits (530), Expect = 3.2e-50
Identity = 102/115 (88.70%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 1   MPIHFALSLLTLLILCTAAEPRQPSIRDQSG-STELWCVAKNNADDASLQSALDWACGAG 60
           MP +F+L LL LLI CTAA+PR+PSIRDQSG +TELWCVAKNNADDASLQSALDWACGAG
Sbjct: 59  MPNNFSLFLLILLIPCTAADPRRPSIRDQSGVATELWCVAKNNADDASLQSALDWACGAG 118

Query: 61  GADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPS 115
           GADCSPIQPGGACYDA+DVQNMASFAFNDYF KHGMTDDSCFFQNSAA+TSLNPS
Sbjct: 119 GADCSPIQPGGACYDAADVQNMASFAFNDYFRKHGMTDDSCFFQNSAAMTSLNPS 173

[Close]

BLAST of Lag0015175 vs. NCBI nr
Match: XP_022989095.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 208.4 bits (529), Expect = 4.2e-50
Identity = 99/114 (86.84%), Postives = 107/114 (93.86%), Query Frame = 0


Query: 1   MPIHFALSLLTLLILCTAAEPRQPSIRDQSGSTELWCVAKNNADDASLQSALDWACGAGG 60
           MPI+FAL LL + ILC+AAEP+ PSI+ Q G+TELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MPINFALFLLIVPILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  ADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPS 115
           ADCSPIQP GACYDAS+VQNMASFAFNDYFLKHGMTDDSCFFQNSAA+TSLNPS
Sbjct: 61  ADCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPS 114

[Close]

BLAST of Lag0015175 vs. NCBI nr
Match: XP_022989094.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 208.4 bits (529), Expect = 4.2e-50
Identity = 99/114 (86.84%), Postives = 107/114 (93.86%), Query Frame = 0


Query: 1   MPIHFALSLLTLLILCTAAEPRQPSIRDQSGSTELWCVAKNNADDASLQSALDWACGAGG 60
           MPI+FAL LL + ILC+AAEP+ PSI+ Q G+TELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MPINFALFLLIVPILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  ADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPS 115
           ADCSPIQP GACYDAS+VQNMASFAFNDYFLKHGMTDDSCFFQNSAA+TSLNPS
Sbjct: 61  ADCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPS 114

[Close]

BLAST of Lag0015175 vs. NCBI nr
Match: XP_023529866.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206.8 bits (525), Expect = 1.2e-49
Identity = 99/114 (86.84%), Postives = 107/114 (93.86%), Query Frame = 0


Query: 1   MPIHFALSLLTLLILCTAAEPRQPSIRDQSGSTELWCVAKNNADDASLQSALDWACGAGG 60
           M I+FAL LL +LILC+AAEP+ PSI+ Q G+TELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MAINFALFLLIVLILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  ADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPS 115
           A+CSPIQP GACYDAS+VQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPS
Sbjct: 61  AECSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPS 114

[Close]

BLAST of Lag0015175 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 8.5e-30
Identity = 66/116 (56.90%), Postives = 84/116 (72.41%), Query Frame = 0


Query: 4   HFALSLL----TLLILCTAAEPRQPSIRDQSGSTELWCVAKNNADDASLQSALDWACGAG 63
           H  LS+L     +L++ T A+     I  Q    ELWCVAKNNA+D+SLQ+A++WACG G
Sbjct: 10  HLRLSILAATAAMLMVITTAQ-----IGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQG 69

Query: 64  GADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSQ 116
           GADC PIQ GG C D +DVQ MASF FN+Y+LK+G  D++C F N+AA+TSLNPSQ
Sbjct: 70  GADCGPIQQGGPCNDPTDVQKMASFVFNNYYLKNGEEDEACNFNNNAALTSLNPSQ 120

[Close]

BLAST of Lag0015175 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 87.0 bits (214), Expect = 1.8e-16
Identity = 39/83 (46.99%), Postives = 56/83 (67.47%), Query Frame = 0


Query: 32  STELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASDVQNMASFAFNDYFL 91
           + + +C+A +  D  +LQ+ALDWACG G ++CS IQPG +CY  ++V+  ASFAFN Y+ 
Sbjct: 377 TNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQ 436

Query: 92  KHGMTDDSCFFQNSAAITSLNPS 115
           K G    SC F+  A IT+ +PS
Sbjct: 437 KEGRASGSCDFKGVAMITTTDPS 459

[Close]

BLAST of Lag0015175 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.9e-15
Identity = 39/82 (47.56%), Postives = 54/82 (65.85%), Query Frame = 0


Query: 33  TELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASDVQNMASFAFNDYFLK 92
           + ++CVAK +ADD  L   L+WACG G A+C+ IQPG  CY  +DV++ ASFAFNDY+ K
Sbjct: 359 SSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQK 418

Query: 93  HGMTDDSCFFQNSAAITSLNPS 115
                 +C F  +A  T+ +PS
Sbjct: 419 MKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of Lag0015175 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 2.2e-14
Identity = 37/90 (41.11%), Postives = 55/90 (61.11%), Query Frame = 0


Query: 25  SIRDQSGSTELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASDVQNMASF 84
           S+     + + +C A+  AD   LQ+ALDWACG G  DCSPI+ G  CY+  +V   A++
Sbjct: 357 SVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANY 416

Query: 85  AFNDYFLKHGMTDDSCFFQNSAAITSLNPS 115
           AF+ Y+ + G   D+C F   A+IT+ +PS
Sbjct: 417 AFDTYYHQTGNNPDACNFNGVASITTTDPS 446

[Close]

BLAST of Lag0015175 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 2.2e-14
Identity = 38/79 (48.10%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 36  WCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASDVQNMASFAFNDYFLKHGM 95
           WCV K  A +  LQ++LDWACG  G DC  IQPGGAC++ ++V + A++A N YF K   
Sbjct: 364 WCVPKKGATNEELQASLDWACG-HGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPK 423

Query: 96  TDDSCFFQNSAAITSLNPS 115
               C F  +A +TS NPS
Sbjct: 424 QPTDCDFSKTATVTSQNPS 441

The following BLAST results are available for this feature:

BLAST of Lag0015175 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0061642.1	4.3e-63	84.83	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 [Cucumis melo var. makuwa] >TYK05864.1 P...	[more]
XP_038877601.1	3.2e-50	88.70	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Benincasa hispida]	[more]
XP_022989095.1	4.2e-50	86.84	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita maxima]	[more]
XP_022989094.1	4.2e-50	86.84	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita maxima]	[more]
XP_023529866.1	1.2e-49	86.84	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X3 [Cucurbita pepo subsp. p...	[more]

BLAST of Lag0015175 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2K6	8.5e-30	56.90	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5...	[more]
O65399	1.8e-16	46.99	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q94CD8	5.9e-15	47.56	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q9C7U5	2.2e-14	41.11	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
Q9M069	2.2e-14	48.10	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 35..119 e-value: 1.7E-36 score: 137.3
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 35..106 e-value: 1.4E-19 score: 70.5
None	No IPR available	GENE3D	1.20.58.1040		coord: 29..128 e-value: 1.4E-11 score: 46.6
None	No IPR available	PANTHER	PTHR31044:SF80	PLASMODESMATA CALLOSE-BINDING PROTEIN 5	coord: 3..124
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 3..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015175.1	Lag0015175.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009506	plasmodesma