Lag0015090 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015090
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Bet_v_1 domain-containing protein
Location	chr12: 7493601 .. 7494632 (+)
RNA-Seq Expression	Lag0015090
Synteny	Lag0015090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAGCATGAAAGGAGAGGTGTCACTGAACCTGCCCGCTGAGAAAGCATGGCAGATGTACAGAGACAACGAGGTGGTCAGTAAAATCAATCCTGAGCTGCTTTCTCGAGCTGAATATGTTCAAGGAGATGGTGGCCCTGGAACTCTCAGGCTTTTCAAGCTTGGCCCTGGTATCTTTCTCTCTCAAAAATTCTTGTTTCAATTTCTTGTACTGCATATACTTGCAAAAAGCTGTAATCTTCTTTTTGGTCCACATGAGCCCATGAAAAGAGGGAGTTATGGGCAAAAGGTCAAAGGCAAAGAAGCTATAGCTGAATTCTTAATCTTTTAACCTTAGGTAGAAGATTCGAACCTCCACTATCATCTCAACCAGGTTGAGAGGTATATATGTTTCAACTGGTATATATGTTTTAACTAGTTGAGTGTAACTTTTTTTTTACTTCCACGTGTGGAAGGACTTGATTAAAGGTATATATATCTTAATCAGGTGGGTATAATTTTCTAGTCCAACAATGGAGTGAGAGATTCAATAACCTAATATTAGGTACAAGTCTTAACTAATTGAATTATGTTTAGATGAGTTGTGTGATTAAGTCCTAGAAAAGGTAAAAAGATGCTGATTATAAGAACACCACAACCTTTCAAAAAAAAAAAAAAAAACCACAACATAACATTTCTAATATCTTTGTTTACAAGAAAATAGTGTGCTGTCATATATGAGTTTTTGATTATGATAACACGTGCAGCTGTGAGAAGCTACGTGGAGGAATCAGTGGAGAAGATAGAGAAGGTGGAGGCAGGTCGATCAGTAAGCTACGATGTGGTGGGAGGAGAGCTAAGGAAGATGTATAATCCCTATAAAGTGACATTCACATTCACTCCGGTGGAAGGAAAAGAGAAGGAAATGTGCATTGCTCAGTGGAAAGCTGAATATGAGCCACTGACTCCCTCCATCCCTCCCCCGGAGAAGGCAAGGGATGCTGCCTTGCGATTTCTCCAGTGCTTTGACAAGTTTCAGCTCAGCTACTAA

mRNA sequence

ATGAGGAGCATGAAAGGAGAGGTGTCACTGAACCTGCCCGCTGAGAAAGCATGGCAGATGTACAGAGACAACGAGGTGGTCAGTAAAATCAATCCTGAGCTGCTTTCTCGAGCTGAATATGTTCAAGGAGATGGTGGCCCTGGAACTCTCAGGCTTTTCAAGCTTGGCCCTGCTGTGAGAAGCTACGTGGAGGAATCAGTGGAGAAGATAGAGAAGGTGGAGGCAGGTCGATCAGTAAGCTACGATGTGGTGGGAGGAGAGCTAAGGAAGATGTATAATCCCTATAAAGTGACATTCACATTCACTCCGGTGGAAGGAAAAGAGAAGGAAATGTGCATTGCTCAGTGGAAAGCTGAATATGAGCCACTGACTCCCTCCATCCCTCCCCCGGAGAAGGCAAGGGATGCTGCCTTGCGATTTCTCCAGTGCTTTGACAAGTTTCAGCTCAGCTACTAA

Coding sequence (CDS)

ATGAGGAGCATGAAAGGAGAGGTGTCACTGAACCTGCCCGCTGAGAAAGCATGGCAGATGTACAGAGACAACGAGGTGGTCAGTAAAATCAATCCTGAGCTGCTTTCTCGAGCTGAATATGTTCAAGGAGATGGTGGCCCTGGAACTCTCAGGCTTTTCAAGCTTGGCCCTGCTGTGAGAAGCTACGTGGAGGAATCAGTGGAGAAGATAGAGAAGGTGGAGGCAGGTCGATCAGTAAGCTACGATGTGGTGGGAGGAGAGCTAAGGAAGATGTATAATCCCTATAAAGTGACATTCACATTCACTCCGGTGGAAGGAAAAGAGAAGGAAATGTGCATTGCTCAGTGGAAAGCTGAATATGAGCCACTGACTCCCTCCATCCCTCCCCCGGAGAAGGCAAGGGATGCTGCCTTGCGATTTCTCCAGTGCTTTGACAAGTTTCAGCTCAGCTACTAA

Protein sequence

MRSMKGEVSLNLPAEKAWQMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVRSYVEESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEYEPLTPSIPPPEKARDAALRFLQCFDKFQLSY

Homology

[Close]

BLAST of Lag0015090 vs. NCBI nr
Match: XP_022147870.1 (uncharacterized protein LOC111016704 [Momordica charantia])

HSP 1 Score: 298.9 bits (764), Expect = 2.5e-77
Identity = 143/151 (94.70%), Postives = 147/151 (97.35%), Query Frame = 0


Query: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR 60
           MRSMKGEV LNLPAEKAWQMYRDN+VVSKINPELLSRAEYV GDGGPGTLRLFKLGPAVR
Sbjct: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60

Query: 61  SYVEESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120
            YVEESVEKIEKVE GRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMC+AQWKAEY
Sbjct: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120

Query: 121 EPLTPSIPPPEKARDAALRFLQCFDKFQLSY 152
           EPLTP+IPPPEKARDAAL+FLQCFDKFQLSY
Sbjct: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 151

[Close]

BLAST of Lag0015090 vs. NCBI nr
Match: XP_038887757.1 (major strawberry allergen Fra a 1.08-like [Benincasa hispida])

HSP 1 Score: 295.8 bits (756), Expect = 2.1e-76
Identity = 141/151 (93.38%), Postives = 148/151 (98.01%), Query Frame = 0


Query: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR 60
           MRSMKGEV L LPAEKAW+MYRDN+VVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR
Sbjct: 1   MRSMKGEVLLKLPAEKAWEMYRDNDVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR 60

Query: 61  SYVEESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120
           SYVEESVEKIEKVE GRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQW+AEY
Sbjct: 61  SYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWEAEY 120

Query: 121 EPLTPSIPPPEKARDAALRFLQCFDKFQLSY 152
           EP+TP+IPPP+KARDAAL+FLQCFDKFQLSY
Sbjct: 121 EPITPAIPPPDKARDAALQFLQCFDKFQLSY 151

[Close]

BLAST of Lag0015090 vs. NCBI nr
Match: XP_022969285.1 (uncharacterized protein LOC111468337 [Cucurbita maxima])

HSP 1 Score: 293.1 bits (749), Expect = 1.4e-75
Identity = 140/151 (92.72%), Postives = 146/151 (96.69%), Query Frame = 0


Query: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR 60
           MRSMKGEVSLNLPAEKAWQMYRDNEV+SKINPELLSRAEYVQGDGGPGTLRLFKLGPA+R
Sbjct: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVISKINPELLSRAEYVQGDGGPGTLRLFKLGPALR 60

Query: 61  SYVEESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120
           SYV ESVEKIEKVE GRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKE EMC AQW+AEY
Sbjct: 61  SYVGESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKETEMCTAQWQAEY 120

Query: 121 EPLTPSIPPPEKARDAALRFLQCFDKFQLSY 152
           EPL+P+IPPP+KARDAALRFLQCFDKF LSY
Sbjct: 121 EPLSPTIPPPDKARDAALRFLQCFDKFDLSY 151

[Close]

BLAST of Lag0015090 vs. NCBI nr
Match: XP_008455315.1 (PREDICTED: uncharacterized protein LOC103495511 [Cucumis melo])

HSP 1 Score: 290.8 bits (743), Expect = 6.7e-75
Identity = 140/151 (92.72%), Postives = 146/151 (96.69%), Query Frame = 0


Query: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR 60
           M+SMKGEV LNLPA+KAWQMYRDN+VVSKINPELLSRAEYVQGDG PGTLRLFKLGPAV 
Sbjct: 1   MKSMKGEVLLNLPAQKAWQMYRDNDVVSKINPELLSRAEYVQGDGSPGTLRLFKLGPAVS 60

Query: 61  SYVEESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120
           SYVEESVEKIEKVE GRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY
Sbjct: 61  SYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120

Query: 121 EPLTPSIPPPEKARDAALRFLQCFDKFQLSY 152
           EPLTP+IPPP+KARDAAL+FLQ FDKFQLSY
Sbjct: 121 EPLTPTIPPPDKARDAALQFLQSFDKFQLSY 151

[Close]

BLAST of Lag0015090 vs. NCBI nr
Match: XP_022952445.1 (uncharacterized protein LOC111455132 [Cucurbita moschata] >XP_022952446.1 uncharacterized protein LOC111455132 [Cucurbita moschata] >XP_023554634.1 uncharacterized protein LOC111811825 [Cucurbita pepo subsp. pepo] >KAG6572365.1 MLP-like protein 423, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 289.3 bits (739), Expect = 2.0e-74
Identity = 138/151 (91.39%), Postives = 145/151 (96.03%), Query Frame = 0


Query: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR 60
           MRSMKGEVSLNLPAEKAWQMYRDNEV+SKINPELLSRAEYVQGDGGPGTLRLFKLGPA+R
Sbjct: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVISKINPELLSRAEYVQGDGGPGTLRLFKLGPALR 60

Query: 61  SYVEESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120
           SYV ESVEKIEKVE GRSVSYDVVGGEL KMYNPYKVTFTFTPVEGKE EMC AQW+AEY
Sbjct: 61  SYVGESVEKIEKVETGRSVSYDVVGGELMKMYNPYKVTFTFTPVEGKETEMCTAQWQAEY 120

Query: 121 EPLTPSIPPPEKARDAALRFLQCFDKFQLSY 152
           EPL+P+IPPP+KARDAALRFLQCFDKF LS+
Sbjct: 121 EPLSPTIPPPDKARDAALRFLQCFDKFDLSF 151

[Close]

BLAST of Lag0015090 vs. ExPASy Swiss-Prot
Match: Q93VR4 (MLP-like protein 423 OS=Arabidopsis thaliana OX=3702 GN=MLP423 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.2e-11
Identity = 45/146 (30.82%), Postives = 71/146 (48.63%), Query Frame = 0


Query: 7   EVSLNLPAEKAWQMYRDN-EVVSKINPELLSRAEYVQGDG-GPGTLRLFKLGPAVRSYVE 66
           EV +  PAEK W    D   +  K  P      + + GDG  PG++RL   G      V+
Sbjct: 10  EVEVKSPAEKFWVALGDGINLFPKAFPNDYKTIQVLAGDGNAPGSIRLITYGEG-SPLVK 69

Query: 67  ESVEKIEKVE-AGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEYEPL 126
            S E+IE V+   +S+SY ++GGE+ + Y  +K T T  P +G      + +W  E+E  
Sbjct: 70  ISAERIEAVDLENKSMSYSIIGGEMLEYYKTFKGTITVIPKDGGS----LLKWSGEFEKT 129

Query: 127 TPSIPPPEKARDAALRFLQCFDKFQL 150
              I  P   +D A++  +  D++ L
Sbjct: 130 AHEIDDPHVIKDFAVKNFKEIDEYLL 150

[Close]

BLAST of Lag0015090 vs. ExPASy Swiss-Prot
Match: P92918 (Major allergen Api g 1, isoallergen 2 OS=Apium graveolens OX=4045 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.0e-06
Identity = 38/134 (28.36%), Postives = 67/134 (50.00%), Query Frame = 0


Query: 7   EVSLNLPAEKAWQ--MYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR-SYV 66
           E    + AEK +Q  +   + V  K+ P+L+   E ++GDGG GT++L  LG A   + +
Sbjct: 9   EAPSTVSAEKMYQGFLLDMDTVFPKVLPQLIKSVEILEGDGGVGTVKLVHLGEATEYTTM 68

Query: 67  EESVEKIEKVEAGRSVSYDVVGGE-LRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEYEP 126
           ++ V+ I+K  AG + +Y  +GG+ L  +       F   P +G     CI +    Y  
Sbjct: 69  KQKVDVIDK--AGLAYTYTTIGGDILVDVLESVVNEFVVVPTDGG----CIVKNTTIYNT 128

Query: 127 LTPSIPPPEKARDA 137
              ++ P +K ++A
Sbjct: 129 KGDAVLPEDKIKEA 136

[Close]

BLAST of Lag0015090 vs. ExPASy Swiss-Prot
Match: P27538 (Pathogenesis-related protein 2 OS=Petroselinum crispum OX=4043 GN=PR2 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.0e-06
Identity = 36/135 (26.67%), Postives = 69/135 (51.11%), Query Frame = 0


Query: 7   EVSLNLPAE---KAWQMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVRSYV 66
           EV+ ++PA+   K + +  DN ++ K+ P+ +   E + GDGG GT++   LG   +  V
Sbjct: 9   EVASSVPAQTIYKGFLLDMDN-IIPKVLPQAIKSIEIISGDGGAGTIKKVTLGEVSQFTV 68

Query: 67  EESVEKIEKVEA-GRSVSYDVVGGE-LRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEYE 126
            +  ++I++++A     SY ++ G+ L  +       FT  P +G     CI +    Y 
Sbjct: 69  VK--QRIDEIDAEALKYSYSIIEGDLLLGIIESITSKFTVVPTDGG----CIVKNTTIYT 128

Query: 127 PLTPSIPPPEKARDA 137
           P+  ++ P E  ++A
Sbjct: 129 PIGDAVIPEENVKEA 136

[Close]

BLAST of Lag0015090 vs. ExPASy Swiss-Prot
Match: A0A1S3THR8 (Phytohormone-binding protein CSBP OS=Vigna radiata var. radiata OX=3916 GN=CSBP PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 5.3e-06
Identity = 34/151 (22.52%), Postives = 72/151 (47.68%), Query Frame = 0


Query: 1   MRSMKGEVSLNLPAEKAWQMYRDN--EVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPA 60
           ++    +  L++  E  W +   +   VV K+ P ++   + ++GDGG GT+ +F   P 
Sbjct: 2   VKEFNTQTELSVRLEALWAVLSKDFITVVPKVLPHIVKDVQLIEGDGGVGTILIFNFLPE 61

Query: 61  VR-SYVEESVEKIEKVEAGRSVSYDVV-GGELRKMYNPYKVTFTFTPVEGKEKEMCIAQW 120
           V  SY  E + + +  E+   +   V+ GG L +  + YK TF  + +E ++K +   + 
Sbjct: 62  VSPSYQREEITEFD--ESSHEIGLQVIEGGYLSQGLSYYKTTFKLSEIE-EDKTLVNVKI 121

Query: 121 KAEYEPLTPSIPPPEKARDAALRFLQCFDKF 148
             +++        P K   + L +L+  +++
Sbjct: 122 SYDHDSDIEEKVTPTKTSQSTLMYLRRLERY 149

[Close]

BLAST of Lag0015090 vs. ExPASy Swiss-Prot
Match: G7J032 (Phytohormone-binding protein OS=Medicago truncatula OX=3880 GN=MTR_3g055120 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 6.9e-06
Identity = 41/156 (26.28%), Postives = 72/156 (46.15%), Query Frame = 0


Query: 1   MRSMKGEVSLNLPAEKAW--QMYRDNEVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPA 60
           ++    + +LN+  E  W  Q      VV K+ P ++   + ++GDGG GT  +F   P 
Sbjct: 2   IKEFNTQTTLNVGLEALWAAQSKDITLVVPKVLPNIVKDVQVIEGDGGVGTKLIFNFLPG 61

Query: 61  VR--SYVEESVEKIEKVEAGRSVSYDVV-GGELRKMYNPYKVTFTFTPV-EGKEKEMCIA 120
           +   +Y  E + + +  E   ++   VV GG L +  + YK TF F+ + E K       
Sbjct: 62  IAPVNYQREVITEYD--ELSHTIGLQVVEGGYLNQGLSYYKTTFQFSAISENKTLVNVKI 121

Query: 121 QWKAEYEPLTPSIPPPEKARDAALRFLQCFDKFQLS 151
            +  E E +   +  P K  ++ L +L   +KF L+
Sbjct: 122 SYDHESELIEEKV-KPTKTSESTLFYLGQLEKFLLN 154

The following BLAST results are available for this feature:

BLAST of Lag0015090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147870.1	2.5e-77	94.70	uncharacterized protein LOC111016704 [Momordica charantia]	[more]
XP_038887757.1	2.1e-76	93.38	major strawberry allergen Fra a 1.08-like [Benincasa hispida]	[more]
XP_022969285.1	1.4e-75	92.72	uncharacterized protein LOC111468337 [Cucurbita maxima]	[more]
XP_008455315.1	6.7e-75	92.72	PREDICTED: uncharacterized protein LOC103495511 [Cucumis melo]	[more]
XP_022952445.1	2.0e-74	91.39	uncharacterized protein LOC111455132 [Cucurbita moschata] >XP_022952446.1 unchar...	[more]

BLAST of Lag0015090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VR4	3.2e-11	30.82	MLP-like protein 423 OS=Arabidopsis thaliana OX=3702 GN=MLP423 PE=2 SV=1	[more]
P92918	4.0e-06	28.36	Major allergen Api g 1, isoallergen 2 OS=Apium graveolens OX=4045 PE=1 SV=1	[more]
P27538	4.0e-06	26.67	Pathogenesis-related protein 2 OS=Petroselinum crispum OX=4043 GN=PR2 PE=2 SV=1	[more]
A0A1S3THR8	5.3e-06	22.52	Phytohormone-binding protein CSBP OS=Vigna radiata var. radiata OX=3916 GN=CSBP ...	[more]
G7J032	6.9e-06	26.28	Phytohormone-binding protein OS=Medicago truncatula OX=3880 GN=MTR_3g055120 PE=1...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I/Major latex protein	SMART	SM01037	Bet_v_1_2	coord: 3..151 e-value: 7.2E-7 score: 38.8
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 4..136 e-value: 1.4E-14 score: 54.3
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 1..150 e-value: 1.2E-28 score: 102.0
None	No IPR available	PANTHER	PTHR31213	FAMILY NOT NAMED	coord: 3..148
None	No IPR available	PANTHER	PTHR31213:SF97	OS08G0374000 PROTEIN	coord: 3..148
None	No IPR available	CDD	cd07816	Bet_v1-like	coord: 3..141 e-value: 8.11894E-34 score: 114.209
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 1..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015090.1	Lag0015090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0006952	defense response
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0080163	regulation of protein serine/threonine phosphatase activity
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0010427	abscisic acid binding
molecular_function	GO:0004864	protein phosphatase inhibitor activity
molecular_function	GO:0038023	signaling receptor activity