Lag0015089 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0015089 vs. NCBI nr
Match: XP_022968991.1 (two pore calcium channel protein 1A-like [Cucurbita maxima])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 572/607 (94.23%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           A TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY LYLSPVAFDYLPF
Sbjct: 132 ATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTLYLSPVAFDYLPF 191

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG
Sbjct: 192 RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 251

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           KI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 252 KIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 311

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAF LMDTNNVGFINKEQCL LLEELNRYR+LPK+S
Sbjct: 312 YDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLEELNRYRSLPKVS 371

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHS FSKKLK
Sbjct: 372 RDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSVFSKKLK 431

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSP FGYAVSFILILNLVAV+IETTLDI NN GQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 432 AFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFGWIYVVEMALKIY 491

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           +FGF+NYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 492 SFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 551

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+VNAGN  L KTDLD
Sbjct: 552 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLVNAGNATLSKTDLD 611

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           +DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIWTLVYFISFYLITVLLLL
Sbjct: 612 EDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYFISFYLITVLLLL 671

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE
Sbjct: 672 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 731

Query: 601 LDNKDSD 608
           LD KDSD
Sbjct: 732 LD-KDSD 737

BLAST of Lag0015089 vs. NCBI nr
Match: XP_008455316.1 (PREDICTED: two pore calcium channel protein 1B isoform X1 [Cucumis melo])

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 572/607 (94.23%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           AVTL+IL+IHTFFPISYEGAQLYW N LNQLKVICIIIL ADFL+Y LYLSPVAFDYLPF
Sbjct: 137 AVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICIIILVADFLVYTLYLSPVAFDYLPF 196

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           R APYIRV+FFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWLAYVIFEDTQQG
Sbjct: 197 RAAPYIRVIFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWLAYVIFEDTQQG 256

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           K+IFTSFG TLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 257 KLIFTSFGTTLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 316

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL+LLEELNRYRTLPKIS
Sbjct: 317 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLMLLEELNRYRTLPKIS 376

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKIN+NEF DLCNAIALKFQKEDIPSWFEGYPSIYHS FSKKLK
Sbjct: 377 RDDYELIFDELDDSRDFKININEFDDLCNAIALKFQKEDIPSWFEGYPSIYHSSFSKKLK 436

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSPKFGYAVSFILILNLVAV+IETTLDIENNVGQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 437 AFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMALKIY 496

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           A+GFMNYWRDGQNQFDF++TWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 497 AYGFMNYWRDGQNQFDFLITWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 556

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGN  LPKTDLD
Sbjct: 557 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPTLPKTDLD 616

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIW+LVYFISFYLITVLLLL
Sbjct: 617 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVYFISFYLITVLLLL 676

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTKTRSRKVDILLHHMLSAE
Sbjct: 677 NLVVAFVLEAFFAELDIESSENGEEQDQDSDSRKDRPRFVGTKTRSRKVDILLHHMLSAE 736

Query: 601 LDNKDSD 608
           L  KDSD
Sbjct: 737 LGEKDSD 743

BLAST of Lag0015089 vs. NCBI nr
Match: KAG6572364.1 (Two pore calcium channel protein 1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 571/607 (94.07%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1    AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
            A TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY LYLSPVAFDYLPF
Sbjct: 685  ATTLVILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTLYLSPVAFDYLPF 744

Query: 61   RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
            RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG
Sbjct: 745  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 804

Query: 121  KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
            KI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 805  KIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 864

Query: 181  YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
            YDSFKSQLAKQISEMDRLRKSILAKAF LMDTNNVGFINKEQCL LLEELNRYR+LPK+S
Sbjct: 865  YDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLEELNRYRSLPKVS 924

Query: 241  KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
            +DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHS FSKKLK
Sbjct: 925  RDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSVFSKKLK 984

Query: 301  VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
             FVRSP FGYAVSFILILNLVAV+IETTLDI NN GQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 985  AFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFGWIYVVEMALKIY 1044

Query: 361  AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
            +FGF+NYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 1045 SFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 1104

Query: 421  LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
            LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+VNAGN  L +TDLD
Sbjct: 1105 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLVNAGNATLSQTDLD 1164

Query: 481  DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
            +DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIWTLVYFISFYLITVLLLL
Sbjct: 1165 EDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYFISFYLITVLLLL 1224

Query: 541  NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
            NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE
Sbjct: 1225 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 1284

Query: 601  LDNKDSD 608
            LD KDSD
Sbjct: 1285 LD-KDSD 1290

BLAST of Lag0015089 vs. NCBI nr
Match: XP_022952442.1 (two pore calcium channel protein 1A-like [Cucurbita moschata])

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 571/607 (94.07%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           A TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY LYLSPVAFD LPF
Sbjct: 133 ATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTLYLSPVAFDSLPF 192

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG
Sbjct: 193 RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 252

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           KI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 253 KIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 312

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAF LMDTNNVGFINKEQCL LLEELNRYR+LPK+S
Sbjct: 313 YDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLEELNRYRSLPKVS 372

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHS FSKKLK
Sbjct: 373 RDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSVFSKKLK 432

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSP FGYAVSFILILNLVAV+IETTLDI NN GQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 433 AFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFGWIYVVEMALKIY 492

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           +FGF+NYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 493 SFGFLNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 552

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VNAGN  L KTDLD
Sbjct: 553 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVNAGNATLSKTDLD 612

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           +DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIWTLVYFISFYLITVLLLL
Sbjct: 613 EDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYFISFYLITVLLLL 672

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE
Sbjct: 673 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 732

Query: 601 LDNKDSD 608
           LD KDSD
Sbjct: 733 LD-KDSD 738

BLAST of Lag0015089 vs. NCBI nr
Match: XP_031744917.1 (two pore calcium channel protein 1A [Cucumis sativus] >KGN43617.1 hypothetical protein Csa_020586 [Cucumis sativus])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 568/607 (93.57%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           AVTL+IL+IHTFFPISYEGAQLYW N LNQLKVICI IL ADFL+Y LYLSPVAFDYLPF
Sbjct: 132 AVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICITILVADFLVYTLYLSPVAFDYLPF 191

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           R APYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWLAYVIFEDTQQG
Sbjct: 192 RAAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWLAYVIFEDTQQG 251

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           K+IFTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 252 KLIFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 311

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVG+INKEQCL+LLEELNRYR+LPKIS
Sbjct: 312 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGYINKEQCLMLLEELNRYRSLPKIS 371

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKINMNEF DLCNAIALKFQKEDIPSWFEGYPS+YHS  SKKLK
Sbjct: 372 RDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGYPSVYHSGSSKKLK 431

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSPKFGYAVSFIL+LNLVAV+IETTLDIENNVGQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 432 AFVRSPKFGYAVSFILVLNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMALKIY 491

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           A+GFMNYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 492 AYGFMNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 551

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGN  L KT+LD
Sbjct: 552 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPTLSKTELD 611

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSY+ELTGSIW+LVYFISFYLITVLLLL
Sbjct: 612 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYRELTGSIWSLVYFISFYLITVLLLL 671

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTKTRSRKVDILLHHMLSAE
Sbjct: 672 NLVVAFVLEAFFAELDIESSENGEEQDQDKDSRKDRPRFVGTKTRSRKVDILLHHMLSAE 731

Query: 601 LDNKDSD 608
           LD+KDSD
Sbjct: 732 LDDKDSD 738

BLAST of Lag0015089 vs. ExPASy Swiss-Prot
Match: Q75VR0 (Two pore calcium channel protein 1B OS=Nicotiana tabacum OX=4097 GN=TPC1B PE=2 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 1.3e-249
Identity = 434/606 (71.62%), Postives = 513/606 (84.65%), Query Frame = 0

Query: 2   VTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFR 61
           VTLL+L+IH  FPISYEG  LYW++ LN+LKVI ++IL AD ++Y L   P  F YLPFR
Sbjct: 131 VTLLLLIIHILFPISYEGFNLYWRSLLNRLKVILLLILVADIVVYILL--PADFYYLPFR 190

Query: 62  VAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGK 121
           +APY+RVVFFI+NIR LR+S  ILAGMLGTYLNV+AL  LFLLFSSWLAYV FEDT+QGK
Sbjct: 191 IAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSSWLAYVFFEDTRQGK 250

Query: 122 IIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVY 181
             FTS+G TLYQMF+LFTTSNNPDVWIPAYK SRWYCLFFVLYVLLGVYFVTNLILAVVY
Sbjct: 251 TTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVYFVTNLILAVVY 310

Query: 182 DSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISK 241
           DSFKS+L KQ+++ DRLR   L KAFSL+D  N G +N++QC +L EELN+YRTLPKIS 
Sbjct: 311 DSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLFEELNKYRTLPKISG 370

Query: 242 DDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKV 301
           DD++ IF+ELDD+ DFKIN+ EFADLC+AI L+FQKED    FE  P+ YHS  S+KL+ 
Sbjct: 371 DDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEACPNFYHSPASEKLRG 430

Query: 302 FVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYA 361
           F+R   F Y + F+L++NLVAV+IETTLDI+NN GQ  WQ++EF FGW+YV+EM LK+Y 
Sbjct: 431 FIRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWLYVIEMALKVYT 490

Query: 362 FGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLL 421
           +GF NYWRDGQN+FDFIVTW+IVIGET TFVAP  LTFLSNGEWIRYLLIARMLRLIRLL
Sbjct: 491 YGFENYWRDGQNRFDFIVTWVIVIGETTTFVAPDDLTFLSNGEWIRYLLIARMLRLIRLL 550

Query: 422 MHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDD 481
           MHV++YRAF+ATFLTLIPSLMPYLGTIFC+LC YC+LG+Q+FGGIVN GN NL +TDL  
Sbjct: 551 MHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIVNTGNPNLAQTDLAG 610

Query: 482 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLLN 541
           +DYLLFNFNDYPNGMVTLFN+LVMGNWQVWMQSYKELTG+ WT  YF+SFYLI+VL LLN
Sbjct: 611 NDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTSWTYAYFVSFYLISVLWLLN 670

Query: 542 LVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAEL 601
           L+VAFVLEAF AE+D+E+S    + D D +++++RRR VGTKTRS++VD LLHHML +EL
Sbjct: 671 LIVAFVLEAFQAEMDLEASARCVDGD-DKEAKRERRRNVGTKTRSQRVDFLLHHMLRSEL 730

Query: 602 DNKDSD 608
               +D
Sbjct: 731 TECSND 733

BLAST of Lag0015089 vs. ExPASy Swiss-Prot
Match: Q94KI8 (Two pore calcium channel protein 1 OS=Arabidopsis thaliana OX=3702 GN=TPC1 PE=1 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 3.0e-249
Identity = 420/604 (69.54%), Postives = 522/604 (86.42%), Query Frame = 0

Query: 2   VTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFR 61
           +TL IL++HTFFPISYEG++++W + LN +KV C++IL  D L+  LYLSP+AFD+LPFR
Sbjct: 126 ITLAILLVHTFFPISYEGSRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFR 185

Query: 62  VAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGK 121
           +APY+RV+ FI++IR LR+++++L+GMLGTYLN+LAL +LFLLF+SW+A+V+FEDTQQG 
Sbjct: 186 IAPYVRVIIFILSIRELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFEDTQQGL 245

Query: 122 IIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVY 181
            +FTS+GATLYQMFILFTTSNNPDVWIPAYK+SRW  +FFVLYVL+GVYFVTNLILAVVY
Sbjct: 246 TVFTSYGATLYQMFILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVY 305

Query: 182 DSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISK 241
           DSFK QLAKQ+S MD++++ +L KAF L+D++  G I+K QC+ L E+L  YRTLPKISK
Sbjct: 306 DSFKEQLAKQVSGMDQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQLTNYRTLPKISK 365

Query: 242 DDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKV 301
           +++ LIFDELDD+ DFKIN +EFADLC AIAL+FQKE++PS FE +P IYHS  S++L+ 
Sbjct: 366 EEFGLIFDELDDTRDFKINKDEFADLCQAIALRFQKEEVPSLFEHFPQIYHSALSQQLRA 425

Query: 302 FVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYA 361
           FVRSP FGYA+SFILI+N +AVV+ETTLDIE +  QK WQ  EFVFGWIYV+EM LKIY 
Sbjct: 426 FVRSPNFGYAISFILIINFIAVVVETTLDIEESSAQKPWQVAEFVFGWIYVLEMALKIYT 485

Query: 362 FGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLL 421
           +GF NYWR+G N+FDF+VTW+IVIGET TF+ P   TF SNGEWIRYLL+ARMLRLIRLL
Sbjct: 486 YGFENYWREGANRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLL 545

Query: 422 MHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDD 481
           M+VQ+YRAF+ATF+TLIPSLMPYLGTIFCVLC+YC++GVQVFGG+VNAGN  L +T+L +
Sbjct: 546 MNVQRYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKKLFETELAE 605

Query: 482 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLLN 541
           DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWM+SYK+LTG+ W++ YF+SFY+IT+LLLLN
Sbjct: 606 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLN 665

Query: 542 LVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAEL 601
           LVVAFVLEAFF ELD+E  E  + Q QD   +++RRR  G+K+RS++VD LLHHML  EL
Sbjct: 666 LVVAFVLEAFFTELDLEEEE--KCQGQDSQEKRNRRRSAGSKSRSQRVDTLLHHMLGDEL 725

Query: 602 DNKD 606
              +
Sbjct: 726 SKPE 727

BLAST of Lag0015089 vs. ExPASy Swiss-Prot
Match: Q75VR1 (Two pore calcium channel protein 1A OS=Nicotiana tabacum OX=4097 GN=TPC1A PE=2 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 5.0e-249
Identity = 431/600 (71.83%), Postives = 511/600 (85.17%), Query Frame = 0

Query: 2   VTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFR 61
           VTLL+L+IH  FPISYEG  LYW++ LN++KVI ++IL AD ++Y L+L+   F YLPFR
Sbjct: 131 VTLLLLIIHILFPISYEGFNLYWRSLLNRVKVILLLILVADIVVYILFLAD--FYYLPFR 190

Query: 62  VAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGK 121
           +APY+RVVFFI+NIR LR+S  ILAGMLGTYLNV+AL  LFLLFSSWLAYV FEDT+QGK
Sbjct: 191 IAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSSWLAYVFFEDTRQGK 250

Query: 122 IIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVY 181
             FTS+G TLYQMF+LFTTSNNPDVWIPAYK SRWYCLFFVLYVLLGVYFVTNLILAVVY
Sbjct: 251 TTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVYFVTNLILAVVY 310

Query: 182 DSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISK 241
           DSFKS+L KQ+++ DRLR   L KAFSL+D  N G +N++QC +L EELN+YRTLPKIS 
Sbjct: 311 DSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQCTLLFEELNKYRTLPKISG 370

Query: 242 DDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKV 301
           DD++ IF ELDD+ DFKIN++EFADLC AI L+FQKED    FE  P+ YHS  S+KL+ 
Sbjct: 371 DDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEACPNFYHSPASEKLRG 430

Query: 302 FVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYA 361
           FVR   F Y + F+L++NLVAV+IETTLDI+NN GQ  WQ++EF FGW+YV+EM LK+Y 
Sbjct: 431 FVRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWLYVIEMALKVYT 490

Query: 362 FGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLL 421
           +GF NYWRDGQN+FDF+VTW+IVIGET TFVAP GLTFLSNGEWIRYLLIARMLRLIRLL
Sbjct: 491 YGFENYWRDGQNRFDFVVTWVIVIGETATFVAPDGLTFLSNGEWIRYLLIARMLRLIRLL 550

Query: 422 MHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDD 481
           MHV++YRAF+ATF TLIPSL+PYLGTIFC+LC YC+LG+Q+FGGIVN GN NL +TDL  
Sbjct: 551 MHVERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGIVNTGNPNLAQTDLAG 610

Query: 482 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLLN 541
           +DYLLFNFNDYPNGMVTLFN+LVMGNWQVWMQSYKELTG+ WT  YF+SFYLI+VL LLN
Sbjct: 611 NDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTAWTYAYFVSFYLISVLWLLN 670

Query: 542 LVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAEL 601
           L+VAFVLEAF AE+D+E+S    + D D +++ +RRR VGTKTRS++VD LLHHML +EL
Sbjct: 671 LIVAFVLEAFQAEVDLEASARCVDGD-DKEAKSERRRNVGTKTRSQRVDFLLHHMLRSEL 727

BLAST of Lag0015089 vs. ExPASy Swiss-Prot
Match: Q5QM84 (Two pore calcium channel protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPC1 PE=1 SV=2)

HSP 1 Score: 796.2 bits (2055), Expect = 2.6e-229
Identity = 400/605 (66.12%), Postives = 488/605 (80.66%), Query Frame = 0

Query: 2   VTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFR 61
           +TL+ILV+  F+P+SYEG  L+WKN +N+LKV+ + ILA D L++       AF   PFR
Sbjct: 158 LTLVILVMDIFYPLSYEGLNLFWKNTINKLKVLLLFILACDILVF-------AFSPQPFR 217

Query: 62  VAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGK 121
           VAPYIRV F IMNIR LR   + L GM+GTYLNVLAL LLFLLF+SWLAYV FEDT QGK
Sbjct: 218 VAPYIRVAFLIMNIRELRMCAVTLVGMVGTYLNVLALSLLFLLFASWLAYVTFEDTPQGK 277

Query: 122 IIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVY 181
            +F+S+G TLYQMFILFTTSNNPDVW+PAYK+SRW  LFF++YVLLGVYF+TNLILAV+Y
Sbjct: 278 TVFSSYGTTLYQMFILFTTSNNPDVWVPAYKSSRWSSLFFIVYVLLGVYFLTNLILAVIY 337

Query: 182 DSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISK 241
           DSFK QLAKQ+S+ D  RKSIL KAF ++D    G++NKEQCL LL+ELN+YR+LPK S+
Sbjct: 338 DSFKEQLAKQVSQADCTRKSILEKAFGIIDATGQGYLNKEQCLSLLDELNKYRSLPKTSR 397

Query: 242 DDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKV 301
           +D+ELIF ELD S DFK+   EFA LCN IA+KFQKE  PS+ E YPS YHS   + LK 
Sbjct: 398 EDFELIFAELDQSGDFKVTSEEFATLCNTIAIKFQKEPPPSYLEKYPSFYHSALCEWLKS 457

Query: 302 FVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYA 361
           FVRSP F Y V F+L++NLVAV+IETTLDIEN+  QK WQE+EFVFGWIYV+EM LKI++
Sbjct: 458 FVRSPLFEYIVIFVLLMNLVAVIIETTLDIENSSSQKVWQEVEFVFGWIYVIEMALKIFS 517

Query: 362 FGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLL 421
            GF  YW +GQN+FDF++TW I IGET+TF  PS L+FLSNGEWIRYLL+ RMLRL R+L
Sbjct: 518 LGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLSFLSNGEWIRYLLLGRMLRLTRIL 577

Query: 422 MHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDD 481
           + V+++RAF+ATF TL+ SLMPYLG +FC LC+YC+LG+Q+FGGIV AGN  L +TDL  
Sbjct: 578 LQVRRFRAFVATFFTLMSSLMPYLGIVFCTLCIYCSLGLQIFGGIVYAGNPTLEETDLFS 637

Query: 482 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLLN 541
           +DYLLFNFNDYP+GMVTLFNLLVMGNWQ WM+SY++LTGS W+L+YF+SFYLI+VLLLLN
Sbjct: 638 NDYLLFNFNDYPSGMVTLFNLLVMGNWQAWMESYRQLTGSYWSLIYFVSFYLISVLLLLN 697

Query: 542 LVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAEL 601
           L+VAFVLEAFFAE+++E     + QD   + R +RRR V  +T+   VDILLHHMLS EL
Sbjct: 698 LIVAFVLEAFFAEMELEKDGEADIQDPTLEGR-NRRRSVRVRTKGTMVDILLHHMLSNEL 754

Query: 602 DNKDS 607
           D   +
Sbjct: 758 DGSQN 754

BLAST of Lag0015089 vs. ExPASy Swiss-Prot
Match: Q6YLX9 (Two pore calcium channel protein 1 OS=Triticum aestivum OX=4565 GN=TPC1 PE=2 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 1.9e-219
Identity = 383/605 (63.31%), Postives = 483/605 (79.83%), Query Frame = 0

Query: 2   VTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFR 61
           +TL+ILV+  F P+SYEG  ++W++  N+LK++ + ILA D L++       AF   PFR
Sbjct: 146 LTLVILVMDIFCPLSYEGLNIFWRSTTNKLKIVLLFILACDILVF-------AFSSQPFR 205

Query: 62  VAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGK 121
           +APYIRVVF IM IR LR   I LAG++GTYLNVLAL LLFLLF+SWLAYV FEDT QGK
Sbjct: 206 LAPYIRVVFLIMTIRELRMCAITLAGLIGTYLNVLALSLLFLLFASWLAYVTFEDTPQGK 265

Query: 122 IIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVY 181
            IF+S+G TLYQMF+LFTTSNNPDVW+ AYK  RWY LFF++YVLLGVYF+TNLILAV+Y
Sbjct: 266 TIFSSYGVTLYQMFVLFTTSNNPDVWVHAYKIPRWYSLFFIVYVLLGVYFLTNLILAVIY 325

Query: 182 DSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISK 241
           DSFK Q AKQ+ ++D +RK+IL KAF L+DTNN G++++EQC+ LL ELN+YR+LPK S+
Sbjct: 326 DSFKEQFAKQLVQVDSIRKNILQKAFDLIDTNNRGYLDREQCISLLNELNKYRSLPKTSR 385

Query: 242 DDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKV 301
           +D+ELIF ELD S DFK+   EFADLCN IA+KFQKE  PS+ E +P  YHS    +LK 
Sbjct: 386 EDFELIFAELDRSGDFKVTSEEFADLCNTIAIKFQKEPPPSYLEKFP-FYHSPLCGRLKS 445

Query: 302 FVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYA 361
           FVRS  F Y + F+L++NLVAV+IETTLDIEN+  Q+ WQE+EF  GWIYV EM LKI++
Sbjct: 446 FVRSRMFEYIIVFVLLINLVAVIIETTLDIENSSSQETWQEVEFFLGWIYVAEMALKIFS 505

Query: 362 FGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLL 421
            GF  YW +GQN+FDF++TW I IGET+TF  PS L FLSNGEWIRYLL+ R+LRL R+L
Sbjct: 506 LGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLPFLSNGEWIRYLLLGRVLRLTRIL 565

Query: 422 MHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDD 481
           + VQ++RAF+ATF TL+ SLMPYLG +FCVLC+YC++G+Q+FGGIV AGN  L +TDL +
Sbjct: 566 LQVQRFRAFVATFFTLMSSLMPYLGIVFCVLCMYCSIGLQIFGGIVYAGNPTLEETDLFN 625

Query: 482 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLLN 541
           +DYLLFNFNDYP+GMVTLFNLLVMGNWQVWM+SY +LTG+ W+L+YF+SFYLI++LLLLN
Sbjct: 626 NDYLLFNFNDYPSGMVTLFNLLVMGNWQVWMESYWQLTGTSWSLIYFVSFYLISILLLLN 685

Query: 542 LVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAEL 601
           L+VAFVLEAFFAE+++E  E  + Q+      K +RR +  +++   VDILLHHMLS EL
Sbjct: 686 LIVAFVLEAFFAEMELEKGEEVDIQNPTSGGIK-KRRSMRVRSKGTMVDILLHHMLSNEL 741

Query: 602 DNKDS 607
           D   +
Sbjct: 746 DGSQN 741

BLAST of Lag0015089 vs. ExPASy TrEMBL
Match: A0A6J1HWG3 (two pore calcium channel protein 1A-like OS=Cucurbita maxima OX=3661 GN=LOC111468128 PE=3 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 572/607 (94.23%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           A TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY LYLSPVAFDYLPF
Sbjct: 132 ATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTLYLSPVAFDYLPF 191

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG
Sbjct: 192 RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 251

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           KI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 252 KIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 311

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAF LMDTNNVGFINKEQCL LLEELNRYR+LPK+S
Sbjct: 312 YDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLEELNRYRSLPKVS 371

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHS FSKKLK
Sbjct: 372 RDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSVFSKKLK 431

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSP FGYAVSFILILNLVAV+IETTLDI NN GQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 432 AFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFGWIYVVEMALKIY 491

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           +FGF+NYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 492 SFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 551

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+VNAGN  L KTDLD
Sbjct: 552 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLVNAGNATLSKTDLD 611

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           +DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIWTLVYFISFYLITVLLLL
Sbjct: 612 EDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYFISFYLITVLLLL 671

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE
Sbjct: 672 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 731

Query: 601 LDNKDSD 608
           LD KDSD
Sbjct: 732 LD-KDSD 737

BLAST of Lag0015089 vs. ExPASy TrEMBL
Match: A0A1S3C1V7 (two pore calcium channel protein 1B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495512 PE=3 SV=1)

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 572/607 (94.23%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           AVTL+IL+IHTFFPISYEGAQLYW N LNQLKVICIIIL ADFL+Y LYLSPVAFDYLPF
Sbjct: 137 AVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICIIILVADFLVYTLYLSPVAFDYLPF 196

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           R APYIRV+FFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWLAYVIFEDTQQG
Sbjct: 197 RAAPYIRVIFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWLAYVIFEDTQQG 256

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           K+IFTSFG TLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 257 KLIFTSFGTTLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 316

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL+LLEELNRYRTLPKIS
Sbjct: 317 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLMLLEELNRYRTLPKIS 376

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKIN+NEF DLCNAIALKFQKEDIPSWFEGYPSIYHS FSKKLK
Sbjct: 377 RDDYELIFDELDDSRDFKININEFDDLCNAIALKFQKEDIPSWFEGYPSIYHSSFSKKLK 436

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSPKFGYAVSFILILNLVAV+IETTLDIENNVGQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 437 AFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMALKIY 496

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           A+GFMNYWRDGQNQFDF++TWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 497 AYGFMNYWRDGQNQFDFLITWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 556

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGN  LPKTDLD
Sbjct: 557 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPTLPKTDLD 616

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIW+LVYFISFYLITVLLLL
Sbjct: 617 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVYFISFYLITVLLLL 676

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTKTRSRKVDILLHHMLSAE
Sbjct: 677 NLVVAFVLEAFFAELDIESSENGEEQDQDSDSRKDRPRFVGTKTRSRKVDILLHHMLSAE 736

Query: 601 LDNKDSD 608
           L  KDSD
Sbjct: 737 LGEKDSD 743

BLAST of Lag0015089 vs. ExPASy TrEMBL
Match: A0A6J1GKF4 (two pore calcium channel protein 1A-like OS=Cucurbita moschata OX=3662 GN=LOC111455128 PE=3 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 571/607 (94.07%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           A TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY LYLSPVAFD LPF
Sbjct: 133 ATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTLYLSPVAFDSLPF 192

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG
Sbjct: 193 RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 252

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           KI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 253 KIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 312

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAF LMDTNNVGFINKEQCL LLEELNRYR+LPK+S
Sbjct: 313 YDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLEELNRYRSLPKVS 372

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHS FSKKLK
Sbjct: 373 RDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSVFSKKLK 432

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSP FGYAVSFILILNLVAV+IETTLDI NN GQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 433 AFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFGWIYVVEMALKIY 492

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           +FGF+NYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 493 SFGFLNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 552

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VNAGN  L KTDLD
Sbjct: 553 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVNAGNATLSKTDLD 612

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           +DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIWTLVYFISFYLITVLLLL
Sbjct: 613 EDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYFISFYLITVLLLL 672

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE
Sbjct: 673 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 732

Query: 601 LDNKDSD 608
           LD KDSD
Sbjct: 733 LD-KDSD 738

BLAST of Lag0015089 vs. ExPASy TrEMBL
Match: A0A0A0K5Q7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G048010 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 568/607 (93.57%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           AVTL+IL+IHTFFPISYEGAQLYW N LNQLKVICI IL ADFL+Y LYLSPVAFDYLPF
Sbjct: 132 AVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICITILVADFLVYTLYLSPVAFDYLPF 191

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           R APYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWLAYVIFEDTQQG
Sbjct: 192 RAAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWLAYVIFEDTQQG 251

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           K+IFTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 252 KLIFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 311

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVG+INKEQCL+LLEELNRYR+LPKIS
Sbjct: 312 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGYINKEQCLMLLEELNRYRSLPKIS 371

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKINMNEF DLCNAIALKFQKEDIPSWFEGYPS+YHS  SKKLK
Sbjct: 372 RDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGYPSVYHSGSSKKLK 431

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSPKFGYAVSFIL+LNLVAV+IETTLDIENNVGQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 432 AFVRSPKFGYAVSFILVLNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMALKIY 491

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           A+GFMNYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 492 AYGFMNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 551

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGN  L KT+LD
Sbjct: 552 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPTLSKTELD 611

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSY+ELTGSIW+LVYFISFYLITVLLLL
Sbjct: 612 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYRELTGSIWSLVYFISFYLITVLLLL 671

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTKTRSRKVDILLHHMLSAE
Sbjct: 672 NLVVAFVLEAFFAELDIESSENGEEQDQDKDSRKDRPRFVGTKTRSRKVDILLHHMLSAE 731

Query: 601 LDNKDSD 608
           LD+KDSD
Sbjct: 732 LDDKDSD 738

BLAST of Lag0015089 vs. ExPASy TrEMBL
Match: A0A6J1ERC4 (two pore calcium channel protein 1A-like OS=Cucurbita moschata OX=3662 GN=LOC111437105 PE=3 SV=1)

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 569/607 (93.74%), Postives = 588/607 (96.87%), Query Frame = 0

Query: 1   AVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPF 60
           AVTL+IL+IHTFFPIS+EGAQLYWKNPLNQLKVIC+IIL ADFLIY LYLSPVAFDYLPF
Sbjct: 132 AVTLIILMIHTFFPISFEGAQLYWKNPLNQLKVICLIILVADFLIYTLYLSPVAFDYLPF 191

Query: 61  RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQG 120
           RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWLAYVIFEDTQQG
Sbjct: 192 RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWLAYVIFEDTQQG 251

Query: 121 KIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 180
           K++FTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV
Sbjct: 252 KLLFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVV 311

Query: 181 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKIS 240
           YDSFKSQLAKQISEMDRLRKSILAKAFSLMDT+NVGFINK+QCLILLEELNRYRTLPKIS
Sbjct: 312 YDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKQQCLILLEELNRYRTLPKIS 371

Query: 241 KDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLK 300
           +DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPS FE YPSIY+S  SKKLK
Sbjct: 372 RDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSVFEAYPSIYNSVSSKKLK 431

Query: 301 VFVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIY 360
            FVRSP FGYA+SFILILNLVAV+IETTLDIENNVGQKAWQELEFVFGWIYVVEM LKIY
Sbjct: 432 AFVRSPSFGYAISFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMALKIY 491

Query: 361 AFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 420
           AFGFMNYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRYLLIARMLRLIRL
Sbjct: 492 AFGFMNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRYLLIARMLRLIRL 551

Query: 421 LMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLD 480
           LMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VNAGN  L KTDLD
Sbjct: 552 LMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVNAGNPALSKTDLD 611

Query: 481 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLL 540
           DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIW+ VYFISFYLITVLLLL
Sbjct: 612 DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSHVYFISFYLITVLLLL 671

Query: 541 NLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAE 600
           NLVVAFVLEAFFAELDIESSENGEEQ+QD DSRKDRRRFVGTKTRSRKVDILLHHMLSAE
Sbjct: 672 NLVVAFVLEAFFAELDIESSENGEEQEQDADSRKDRRRFVGTKTRSRKVDILLHHMLSAE 731

Query: 601 LDNKDSD 608
           LD KDSD
Sbjct: 732 LDGKDSD 738

BLAST of Lag0015089 vs. TAIR 10
Match: AT4G03560.1 (two-pore channel 1 )

HSP 1 Score: 862.4 bits (2227), Expect = 2.1e-250
Identity = 420/604 (69.54%), Postives = 522/604 (86.42%), Query Frame = 0

Query: 2   VTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFR 61
           +TL IL++HTFFPISYEG++++W + LN +KV C++IL  D L+  LYLSP+AFD+LPFR
Sbjct: 126 ITLAILLVHTFFPISYEGSRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFR 185

Query: 62  VAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGK 121
           +APY+RV+ FI++IR LR+++++L+GMLGTYLN+LAL +LFLLF+SW+A+V+FEDTQQG 
Sbjct: 186 IAPYVRVIIFILSIRELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFEDTQQGL 245

Query: 122 IIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVY 181
            +FTS+GATLYQMFILFTTSNNPDVWIPAYK+SRW  +FFVLYVL+GVYFVTNLILAVVY
Sbjct: 246 TVFTSYGATLYQMFILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVY 305

Query: 182 DSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISK 241
           DSFK QLAKQ+S MD++++ +L KAF L+D++  G I+K QC+ L E+L  YRTLPKISK
Sbjct: 306 DSFKEQLAKQVSGMDQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQLTNYRTLPKISK 365

Query: 242 DDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKV 301
           +++ LIFDELDD+ DFKIN +EFADLC AIAL+FQKE++PS FE +P IYHS  S++L+ 
Sbjct: 366 EEFGLIFDELDDTRDFKINKDEFADLCQAIALRFQKEEVPSLFEHFPQIYHSALSQQLRA 425

Query: 302 FVRSPKFGYAVSFILILNLVAVVIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYA 361
           FVRSP FGYA+SFILI+N +AVV+ETTLDIE +  QK WQ  EFVFGWIYV+EM LKIY 
Sbjct: 426 FVRSPNFGYAISFILIINFIAVVVETTLDIEESSAQKPWQVAEFVFGWIYVLEMALKIYT 485

Query: 362 FGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLL 421
           +GF NYWR+G N+FDF+VTW+IVIGET TF+ P   TF SNGEWIRYLL+ARMLRLIRLL
Sbjct: 486 YGFENYWREGANRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLL 545

Query: 422 MHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDD 481
           M+VQ+YRAF+ATF+TLIPSLMPYLGTIFCVLC+YC++GVQVFGG+VNAGN  L +T+L +
Sbjct: 546 MNVQRYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKKLFETELAE 605

Query: 482 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGSIWTLVYFISFYLITVLLLLN 541
           DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWM+SYK+LTG+ W++ YF+SFY+IT+LLLLN
Sbjct: 606 DDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLN 665

Query: 542 LVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAEL 601
           LVVAFVLEAFF ELD+E  E  + Q QD   +++RRR  G+K+RS++VD LLHHML  EL
Sbjct: 666 LVVAFVLEAFFTELDLEEEE--KCQGQDSQEKRNRRRSAGSKSRSQRVDTLLHHMLGDEL 725

Query: 602 DNKD 606
              +
Sbjct: 726 SKPE 727