Lag0015070 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0015070 vs. NCBI nr
Match: XP_038888606.1 (uncharacterized protein LOC120078409 [Benincasa hispida])

HSP 1 Score: 705.3 bits (1819), Expect = 4.5e-199
Identity = 390/602 (64.78%), Postives = 439/602 (72.92%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVE 60
           MFG LFGWRKASKCKKLI+ ALCRLQMLKKKRYSIV+QLREDLFEL+NNGYQQIAF RVE
Sbjct: 1   MFGFLFGWRKASKCKKLIRTALCRLQMLKKKRYSIVKQLREDLFELVNNGYQQIAFRRVE 60

Query: 61  QLIRDESLMEAYDLIESFCEFILVKFSHIRKHK------TCPDDIIEAISSLIFASARCG 120
           QLI+DE LMEAYDLIE+FCEFILVKFSHI+KHK      TCPDDIIEAISSLIFASARCG
Sbjct: 61  QLIQDEILMEAYDLIENFCEFILVKFSHIKKHKYFMVFRTCPDDIIEAISSLIFASARCG 120

Query: 121 DFPELKPVRKLFEERYGRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIAR 180
           DFPELK VRKLFE+R+GRSFAVAAVELCPGNLVN QIKEKLL+KPV +HEKQR I++IAR
Sbjct: 121 DFPELKSVRKLFEKRFGRSFAVAAVELCPGNLVNSQIKEKLLLKPVSDHEKQRFINDIAR 180

Query: 181 DCFQPAILALEYCPDWHQKQVLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSD--I 240
           DCF P+ILALEY PDWHQKQV +N D+T+ E KE        E+LE VV S +SS D   
Sbjct: 181 DCFYPSILALEYSPDWHQKQVHDNEDETNGEGKE------EPEKLEKVVISLNSSRDNNR 240

Query: 241 DIVD---------PLVCQSSTNGNEEVYCNSALASSNLLSWQAKECDSSGWIAAATLEDG 300
           D+VD          LVCQSS N  E+V                                G
Sbjct: 241 DVVDARCHSTSSSSLVCQSSPNEREDV--------------------------------G 300

Query: 301 LDRGNGASSPEFSPFREETIIYLDDVIELPNPSTEDGDLWDQRFFKFRSPVTSMRDNVQH 360
           LDR N ASSPE  PF +E+I+YLD+++EL + S E GD  DQRFFKF+S VTSM +NV+ 
Sbjct: 301 LDRENDASSPEILPFCDESIVYLDNIVELSDLSMEYGDSLDQRFFKFKSLVTSMGENVRD 360

Query: 361 GNDQSLIENHDVSNKKSVSERSIQSINGAQKGSRRRSTCRERENHSSSHTKKKLTKCCCP 420
           GNDQSLIE HD SN K VS RS Q I G+ K  R  S CRE+ENHS ++TKKK  KCCC 
Sbjct: 361 GNDQSLIEKHDDSNTKLVSGRSNQIIIGSPKELRIVSMCREQENHSLNNTKKKFMKCCCL 420

Query: 421 SCHSLLSEMENYCLEQQCYVYSECRTDDYEVVISEKKKADLYFIRSSADNRFDYDLSCKD 480
           SCHSL SE++NYCLEQ CYVYSECRTDD             Y +RSSA    DYDLS KD
Sbjct: 421 SCHSLSSELQNYCLEQPCYVYSECRTDD-------------YVVRSSAVKHTDYDLSSKD 480

Query: 481 SPPNKDHIPWNGDSDEELEFDTFSMTKKMRSCEIGTVVYDVFVYSQCKPDENMETNAKPE 540
           S        WN DS+EE+EFDTFS TKK  +   GTVVYDVFVYS C+P EN ETNAKPE
Sbjct: 481 S--------WNEDSNEEIEFDTFSRTKKASNYGNGTVVYDVFVYSHCQPVENKETNAKPE 540

Query: 541 EFRTKGKQESSFDFNGMESNITKCMKAADKYPSHVHPKLPDYEEIADKFIALKRGHMQQQ 586
           E RT GK E+S  FNG+++  TKCMK ADKYPSHVHPKLPDY+EIA KFIALKR ++Q+ 
Sbjct: 541 ELRTNGKYETSVGFNGIQNKFTKCMKGADKYPSHVHPKLPDYDEIAAKFIALKREYLQKS 543

BLAST of Lag0015070 vs. NCBI nr
Match: XP_022147829.1 (uncharacterized protein LOC111016673 [Momordica charantia])

HSP 1 Score: 672.9 bits (1735), Expect = 2.4e-189
Identity = 379/576 (65.80%), Postives = 420/576 (72.92%), Query Frame = 0

Query: 17  LIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLMEAYDLIE 76
           +IKRAL RL+MLKKKRYSI+RQLREDL ELI+NGYQQIAFNRVEQLIRDESLMEAYDLIE
Sbjct: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60

Query: 77  SFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERYGRSFAV 136
           +FCEFIL+ FSH+RKHKTCPDD+IEAISSLIFASARCGD PELK VRKLFEER+GRSFA 
Sbjct: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120

Query: 137 AAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPAILALEYCPDWHQKQVL 196
            AVELCPGNLVNPQIKEKLLVKPV +HEKQ LISEIARDCF+PAILALEYCPDWHQKQVL
Sbjct: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180

Query: 197 ENGDQTHCERK-EPKVQDSNAEELETVVSSRDSSSDIDIVDPLVCQSSTNGNEEVYCNSA 256
           ENGDQTHCE K EP +QDSNAEE E  V     S +       VC SS            
Sbjct: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQE-------VCSSS------------ 240

Query: 257 LASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFREETIIYLDDVIELPNP 316
                                 ATLED       ASSPE SPF EET++YLDDV+ELPNP
Sbjct: 241 ----------------------ATLED-------ASSPECSPFCEETLVYLDDVVELPNP 300

Query: 317 STEDGDLWDQRFFKFRSPVTSMRDNVQHGNDQSLI-ENHDVSNKKSVSERSIQSINGAQK 376
           S E+G LWDQR FKF+SPVT MR+NVQ+GN Q LI E+H+ S K+SVSERS QS+N + K
Sbjct: 301 SMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVSERSSQSMNKSPK 360

Query: 377 GSRRRSTCRERENHSSSHTKKKLTKCCCPSCHS--LLSEMENYCLEQQCYVYSECRTDDY 436
            S RRST   +E+ S SH KK+L KCCC SCHS  L S MENYCLEQ  YVYS       
Sbjct: 361 RSMRRST--HQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSYVYS------- 420

Query: 437 EVVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDEELEFDTFSMTKKM 496
           EV+ISEKKK D +FI        D  LS KD                 +EFDT   T+K+
Sbjct: 421 EVLISEKKKVDPHFI-------LDNSLSGKDR--------------HRVEFDTLPRTEKI 480

Query: 497 RSCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNGMESNITKCMKAAD 556
           R+CE+GT+VYDVFVYS C+P EN ETNAKPEE  TK K ESS  FNGM+S+ TKC KAAD
Sbjct: 481 RNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAAD 496

Query: 557 KYPSHVHPKLPDYEEIADKFIALKRGHMQQQRKQHQ 589
           KYPSHVHPKLPDY+EIA KFIALKR +   QRKQHQ
Sbjct: 541 KYPSHVHPKLPDYDEIAAKFIALKREY--SQRKQHQ 496

BLAST of Lag0015070 vs. NCBI nr
Match: XP_016901704.1 (PREDICTED: uncharacterized protein LOC103495691 [Cucumis melo])

HSP 1 Score: 637.5 bits (1643), Expect = 1.1e-178
Identity = 362/587 (61.67%), Postives = 414/587 (70.53%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVE 60
           MFG LFGWRKASKCKKLIK ALCRL++LKKKRYSIV+ LREDL ELINNGYQQIAF RVE
Sbjct: 1   MFGFLFGWRKASKCKKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVE 60

Query: 61  QLIRDESLMEAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 120
           QL++DE+LME YDLIE+ CEFILVKFSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK
Sbjct: 61  QLLQDETLMEVYDLIENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELK 120

Query: 121 PVRKLFEERYGRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPA 180
            VR LFEER+G+SFAVAAVEL PGNLVN QIKEKL+++ V  HEKQRLI+EIARDCF PA
Sbjct: 121 CVRMLFEERFGKSFAVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPA 180

Query: 181 ILALEYCPDWHQKQVLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSDIDIVDPLVC 240
           +LALEY PD  QKQVL+N DQT  E K        +EELE VV S DS+ D         
Sbjct: 181 VLALEYRPDSDQKQVLQNEDQTTVEGK------VESEELERVVISLDSARD--------- 240

Query: 241 QSSTNGNEEVYCNSALASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFRE 300
             S  G  + +C+S  +SS++       C S                N ASS EF PF E
Sbjct: 241 --SNCGVLDPWCHSTSSSSSV-------CQSF--------------PNDASSSEFLPFCE 300

Query: 301 ETIIYLDDVIELPNPSTEDGDLWDQRFFKFRSPVTSMR-DNVQHGNDQSLIENHDVSNKK 360
           E I+Y DDV+EL +PSTE GDL DQRFFKF+  +TS R +NV+  +DQSLIE HD SNKK
Sbjct: 301 EAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIEKHDASNKK 360

Query: 361 SVSERSIQSINGAQKGSRRRSTCRERENHSSSHTKKKLTKCCCPSCHSLLSEMENYCLEQ 420
           SVS RS Q ING  K   R+  CRE+ENHS +HTKKK TKCCC SCHSL  E++NY LEQ
Sbjct: 361 SVSGRSNQIINGPPKEPSRKFMCREQENHSLNHTKKKFTKCCCLSCHSLSFELQNYSLEQ 420

Query: 421 QCYVYSECRTDDYEVVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDE 480
           QCYVYSECRT             D Y IRSS  NR DYDLS K       H+ WN +S+E
Sbjct: 421 QCYVYSECRT-------------DYYIIRSSEANRLDYDLSSK-------HL-WNAESNE 480

Query: 481 ELEFDTFSMTKKMRSCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNG 540
           E+EF TFS  K+ R+  IGTVVYDVFVYS C+PDEN ETN K EE               
Sbjct: 481 EIEFITFSRAKEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEEHE------------- 514

Query: 541 MESNITKCMKAADKYPSHVHPKLPDYEEIADKFIALKRGHMQQQRKQ 587
             S  TKCMK A KYPSHVHPKLPDY+EIA +FI+LKR ++Q+  K+
Sbjct: 541 -PSKFTKCMKGAHKYPSHVHPKLPDYDEIAVEFISLKREYLQRSMKR 514

BLAST of Lag0015070 vs. NCBI nr
Match: KAE8645768.1 (hypothetical protein Csa_020499 [Cucumis sativus])

HSP 1 Score: 618.2 bits (1593), Expect = 7.2e-173
Identity = 350/572 (61.19%), Postives = 405/572 (70.80%), Query Frame = 0

Query: 15  KKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLMEAYDL 74
           KKLIK ALCRL++L+KKRYSIV+ LREDL ELINNGYQQIAF RVEQLI+DE+LMEAYDL
Sbjct: 238 KKLIKTALCRLKLLRKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLIQDETLMEAYDL 297

Query: 75  IESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERYGRSF 134
           IE+FCE ILVKFSHIRKHKTCPDDI EAISSLIFASAR GDFPELK VRKLFEER+G+SF
Sbjct: 298 IENFCEIILVKFSHIRKHKTCPDDITEAISSLIFASARFGDFPELKCVRKLFEERFGKSF 357

Query: 135 AVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPAILALEYCPDWHQKQ 194
            VAAVEL PGNLVN QIKEKL+++PV  HEKQRLI+EIARDCF PA+LALE CPDWH+KQ
Sbjct: 358 IVAAVELSPGNLVNKQIKEKLVMQPVPNHEKQRLINEIARDCFHPAVLALENCPDWHKKQ 417

Query: 195 VLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSDIDIVDPLVCQSSTNGNEEVYCNS 254
           VL+NGDQT  + K        +EELE VV S DS+ D           S  G  + +C+S
Sbjct: 418 VLQNGDQTTVKGK------VESEELERVVISLDSTRD-----------SNCGVLDSWCHS 477

Query: 255 ALASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFREETIIYLDDVIELPN 314
             +S ++                          N AS+ EF PF EE ++Y DDV+EL +
Sbjct: 478 TSSSPSV---------------------SQSFPNDASTSEFLPFCEEAMVYFDDVVELSD 537

Query: 315 PSTEDGDLWDQRFFKFRSPVTSMR-DNVQHGNDQSLIENHDVSNKKSVSERSIQSINGAQ 374
           PSTE GDL DQRFFKF+S +TS R +NV  G+DQSLIENH  SNKK+VS RS Q ING+ 
Sbjct: 538 PSTEYGDLLDQRFFKFKSLITSTREENVGDGDDQSLIENHYGSNKKAVSGRSNQIINGSP 597

Query: 375 KGSRRRSTCRERENHSSSHTKKKLTKCCCPSCHSLLSEMENYCLEQQCYVYSECRTDDYE 434
           K   RRS  RE+ENHS +HTKKKL KC C SCHSL SE++NYC+EQQCYV+SECRT    
Sbjct: 598 KEPSRRSMRREQENHSLNHTKKKLMKCYCLSCHSLSSELQNYCVEQQCYVHSECRT---- 657

Query: 435 VVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDEELEFDTFSMTKKMR 494
                    D   I+SS  N  DYDLS K+         WN +S+EE+EF TFS  K  R
Sbjct: 658 ---------DFSVIQSSEANNSDYDLSSKNL--------WNAESNEEIEFITFSRAKDQR 717

Query: 495 SCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNGMESNITKCMKAADK 554
           +  IGTVVYDVFVYSQC+PDEN ETN K +E  T GK E         S  TKCMK ADK
Sbjct: 718 NYGIGTVVYDVFVYSQCQPDENKETNTKLKELSTNGKHE--------PSKFTKCMKGADK 742

Query: 555 YPSHVHPKLPDYEEIADKFIALKRGHMQQQRK 586
           YPSHVHPKLP+YEEIA KFI LKR ++++  K
Sbjct: 778 YPSHVHPKLPNYEEIAAKFICLKREYLERSMK 742

BLAST of Lag0015070 vs. NCBI nr
Match: KAA0031582.1 (Ist1 domain-containing protein [Cucumis melo var. makuwa] >TYK07034.1 Ist1 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 607.4 bits (1565), Expect = 1.3e-169
Identity = 349/573 (60.91%), Postives = 400/573 (69.81%), Query Frame = 0

Query: 15  KKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLMEAYDL 74
           KKLIK ALCRL++LKKKRYSIV+ LREDL ELINNGYQQIAF RVEQL++DE+LME YDL
Sbjct: 4   KKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDL 63

Query: 75  IESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERYGRSF 134
           IE+ CEFILVKFSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK VR LFEER+G+SF
Sbjct: 64  IENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSF 123

Query: 135 AVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPAILALEYCPDWHQKQ 194
           AVAAVEL PGNLVN QIKEKL+++ V  HEKQRLI+EIARDCF PA+LALEY PD  QKQ
Sbjct: 124 AVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQ 183

Query: 195 VLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSDIDIVDPLVCQSSTNGNEEVYCNS 254
           VL N DQT  E K        +EELE VV S DS+ D           S  G  + +C+S
Sbjct: 184 VLPNEDQTTVEGK------VESEELERVVISLDSARD-----------SNCGVLDPWCHS 243

Query: 255 ALASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFREETIIYLDDVIELPN 314
             +SS++       C S                N ASS EF PF EE I+Y DDV+EL +
Sbjct: 244 TSSSSSV-------CQSF--------------PNDASSSEFLPFCEEAIVYFDDVVELSD 303

Query: 315 PSTEDGDLWDQRFFKFRSPVTSMR-DNVQHGNDQSLIENHDVSNKKSVSERSIQSINGAQ 374
           PSTE GDL DQRFFKF+  +TS R +NV+  +DQSLIE HD SNKKSVS RS Q ING  
Sbjct: 304 PSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIEKHDASNKKSVSGRSNQIINGPP 363

Query: 375 KGSRRRSTCRERENHSSSHTKKKLTKCCCPSCHSLLSEMENYCLEQQCYVYSECRTDDYE 434
           K   R+  CRE+ENHS +HTKKK TKCCC SCHSL  E++NY LEQQCYVYSECRT    
Sbjct: 364 KEPSRKLMCREQENHSLNHTKKKFTKCCCLSCHSLSFELQNYSLEQQCYVYSECRT---- 423

Query: 435 VVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDEELEFDTFSMTKKMR 494
                    D Y IRSS  NR DYDLS K       H+ WN +S+EE+EF TFS  K+ R
Sbjct: 424 ---------DYYIIRSSEANRLDYDLSSK-------HL-WNAESNEEIEFITFSRAKEQR 483

Query: 495 SCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNGMESNITKCMKAADK 554
           +  IGTVVYDVFVYS C+PDEN ETN K EE                 S  TKCMK A K
Sbjct: 484 NYGIGTVVYDVFVYSHCQPDENKETNTKLEEHE--------------PSKFTKCMKGAHK 503

Query: 555 YPSHVHPKLPDYEEIADKFIALKRGHMQQQRKQ 587
           YPSHVHPKLPDY+EIA +FI+LKR ++Q+  K+
Sbjct: 544 YPSHVHPKLPDYDEIAVEFISLKREYLQRSMKR 503

BLAST of Lag0015070 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 4.6e-13
Identity = 48/143 (33.57%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 13  KCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLMEAY 72
           K K  +K A+ R+Q+LK K+ +IVR  + ++ EL+    ++ A  RVE +IRDE L+E +
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  DLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERYGR 132
            +IE  CE +  + + I      P ++ E+I +L+++S R    PEL+ ++   + +YG+
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 SFAVAAVELCPGNLVNPQIKEKL 156
                A   C  + VNP+I  KL
Sbjct: 132 GLENEANCHCSTH-VNPKIVHKL 152

BLAST of Lag0015070 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.9e-12
Identity = 50/197 (25.38%), Postives = 107/197 (54.31%), Query Frame = 0

Query: 10  KASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLM 69
           KA + +  ++  + RL++L+KK+  + ++ R+++ + +  G  + A  RVE +IR++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  EAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKPVRKLFEE 129
           EA +++E +C+ +L +F  I+  K     + E++S+LI+A+ R   +  ELK V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRSFAVAAVELCPGN---LVNPQIKEKLLVKPVLEHEKQRLISEIARDC---FQPAIL 189
           +Y + +     +LC  N    VN ++  KL V+   +   +R + EIA++    ++P  +
Sbjct: 127 KYSKEYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSV 186

Query: 190 ALEYCPDWHQKQVLENG 200
            +   P   +  +++ G
Sbjct: 187 VMAEAPPGVETDLIDVG 199

BLAST of Lag0015070 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.9e-12
Identity = 50/197 (25.38%), Postives = 107/197 (54.31%), Query Frame = 0

Query: 10  KASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLM 69
           KA + +  ++  + RL++L+KK+  + ++ R+++ + +  G  + A  RVE +IR++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  EAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKPVRKLFEE 129
           EA +++E +C+ +L +F  I+  K     + E++S+LI+A+ R   +  ELK V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRSFAVAAVELCPGN---LVNPQIKEKLLVKPVLEHEKQRLISEIARDC---FQPAIL 189
           +Y + +     +LC  N    VN ++  KL V+   +   +R + EIA++    ++P  +
Sbjct: 127 KYSKEYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSV 186

Query: 190 ALEYCPDWHQKQVLENG 200
            +   P   +  +++ G
Sbjct: 187 VMAEAPPGVETDLIDVG 199

BLAST of Lag0015070 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.9e-12
Identity = 50/197 (25.38%), Postives = 107/197 (54.31%), Query Frame = 0

Query: 10  KASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLM 69
           KA + +  ++  + RL++L+KK+  + ++ R+++ + +  G  + A  RVE +IR++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  EAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKPVRKLFEE 129
           EA +++E +C+ +L +F  I+  K     + E++S+LI+A+ R   +  ELK V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRSFAVAAVELCPGN---LVNPQIKEKLLVKPVLEHEKQRLISEIARDC---FQPAIL 189
           +Y + +     +LC  N    VN ++  KL V+   +   +R + EIA++    ++P  +
Sbjct: 127 KYSKGYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSV 186

Query: 190 ALEYCPDWHQKQVLENG 200
            +   P   +  +++ G
Sbjct: 187 VMAEAPPGVETDLIDVG 199

BLAST of Lag0015070 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 8.6e-12
Identity = 47/170 (27.65%), Postives = 96/170 (56.47%), Query Frame = 0

Query: 10  KASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLM 69
           KA + +  ++  + RL++L+KK+  + ++ R+++ + +  G  + A  RVE +IR++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  EAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKPVRKLFEE 129
           EA +++E +C+ +L +F  I+  K     + E++S+LI+A+ R   +  ELK V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRSFAVAAVELCPGN---LVNPQIKEKLLVKPVLEHEKQRLISEIARD 176
           +Y + +     +LC  N    VN ++  KL V+   +   +R + EIA++
Sbjct: 127 KYSKEYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Lag0015070 vs. ExPASy TrEMBL
Match: A0A6J1D262 (uncharacterized protein LOC111016673 OS=Momordica charantia OX=3673 GN=LOC111016673 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 1.2e-189
Identity = 379/576 (65.80%), Postives = 420/576 (72.92%), Query Frame = 0

Query: 17  LIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLMEAYDLIE 76
           +IKRAL RL+MLKKKRYSI+RQLREDL ELI+NGYQQIAFNRVEQLIRDESLMEAYDLIE
Sbjct: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60

Query: 77  SFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERYGRSFAV 136
           +FCEFIL+ FSH+RKHKTCPDD+IEAISSLIFASARCGD PELK VRKLFEER+GRSFA 
Sbjct: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120

Query: 137 AAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPAILALEYCPDWHQKQVL 196
            AVELCPGNLVNPQIKEKLLVKPV +HEKQ LISEIARDCF+PAILALEYCPDWHQKQVL
Sbjct: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180

Query: 197 ENGDQTHCERK-EPKVQDSNAEELETVVSSRDSSSDIDIVDPLVCQSSTNGNEEVYCNSA 256
           ENGDQTHCE K EP +QDSNAEE E  V     S +       VC SS            
Sbjct: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQE-------VCSSS------------ 240

Query: 257 LASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFREETIIYLDDVIELPNP 316
                                 ATLED       ASSPE SPF EET++YLDDV+ELPNP
Sbjct: 241 ----------------------ATLED-------ASSPECSPFCEETLVYLDDVVELPNP 300

Query: 317 STEDGDLWDQRFFKFRSPVTSMRDNVQHGNDQSLI-ENHDVSNKKSVSERSIQSINGAQK 376
           S E+G LWDQR FKF+SPVT MR+NVQ+GN Q LI E+H+ S K+SVSERS QS+N + K
Sbjct: 301 SMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVSERSSQSMNKSPK 360

Query: 377 GSRRRSTCRERENHSSSHTKKKLTKCCCPSCHS--LLSEMENYCLEQQCYVYSECRTDDY 436
            S RRST   +E+ S SH KK+L KCCC SCHS  L S MENYCLEQ  YVYS       
Sbjct: 361 RSMRRST--HQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSYVYS------- 420

Query: 437 EVVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDEELEFDTFSMTKKM 496
           EV+ISEKKK D +FI        D  LS KD                 +EFDT   T+K+
Sbjct: 421 EVLISEKKKVDPHFI-------LDNSLSGKDR--------------HRVEFDTLPRTEKI 480

Query: 497 RSCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNGMESNITKCMKAAD 556
           R+CE+GT+VYDVFVYS C+P EN ETNAKPEE  TK K ESS  FNGM+S+ TKC KAAD
Sbjct: 481 RNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAAD 496

Query: 557 KYPSHVHPKLPDYEEIADKFIALKRGHMQQQRKQHQ 589
           KYPSHVHPKLPDY+EIA KFIALKR +   QRKQHQ
Sbjct: 541 KYPSHVHPKLPDYDEIAAKFIALKREY--SQRKQHQ 496

BLAST of Lag0015070 vs. ExPASy TrEMBL
Match: A0A1S4E0G2 (uncharacterized protein LOC103495691 OS=Cucumis melo OX=3656 GN=LOC103495691 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 5.5e-179
Identity = 362/587 (61.67%), Postives = 414/587 (70.53%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVE 60
           MFG LFGWRKASKCKKLIK ALCRL++LKKKRYSIV+ LREDL ELINNGYQQIAF RVE
Sbjct: 1   MFGFLFGWRKASKCKKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVE 60

Query: 61  QLIRDESLMEAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 120
           QL++DE+LME YDLIE+ CEFILVKFSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK
Sbjct: 61  QLLQDETLMEVYDLIENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELK 120

Query: 121 PVRKLFEERYGRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPA 180
            VR LFEER+G+SFAVAAVEL PGNLVN QIKEKL+++ V  HEKQRLI+EIARDCF PA
Sbjct: 121 CVRMLFEERFGKSFAVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPA 180

Query: 181 ILALEYCPDWHQKQVLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSDIDIVDPLVC 240
           +LALEY PD  QKQVL+N DQT  E K        +EELE VV S DS+ D         
Sbjct: 181 VLALEYRPDSDQKQVLQNEDQTTVEGK------VESEELERVVISLDSARD--------- 240

Query: 241 QSSTNGNEEVYCNSALASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFRE 300
             S  G  + +C+S  +SS++       C S                N ASS EF PF E
Sbjct: 241 --SNCGVLDPWCHSTSSSSSV-------CQSF--------------PNDASSSEFLPFCE 300

Query: 301 ETIIYLDDVIELPNPSTEDGDLWDQRFFKFRSPVTSMR-DNVQHGNDQSLIENHDVSNKK 360
           E I+Y DDV+EL +PSTE GDL DQRFFKF+  +TS R +NV+  +DQSLIE HD SNKK
Sbjct: 301 EAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIEKHDASNKK 360

Query: 361 SVSERSIQSINGAQKGSRRRSTCRERENHSSSHTKKKLTKCCCPSCHSLLSEMENYCLEQ 420
           SVS RS Q ING  K   R+  CRE+ENHS +HTKKK TKCCC SCHSL  E++NY LEQ
Sbjct: 361 SVSGRSNQIINGPPKEPSRKFMCREQENHSLNHTKKKFTKCCCLSCHSLSFELQNYSLEQ 420

Query: 421 QCYVYSECRTDDYEVVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDE 480
           QCYVYSECRT             D Y IRSS  NR DYDLS K       H+ WN +S+E
Sbjct: 421 QCYVYSECRT-------------DYYIIRSSEANRLDYDLSSK-------HL-WNAESNE 480

Query: 481 ELEFDTFSMTKKMRSCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNG 540
           E+EF TFS  K+ R+  IGTVVYDVFVYS C+PDEN ETN K EE               
Sbjct: 481 EIEFITFSRAKEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEEHE------------- 514

Query: 541 MESNITKCMKAADKYPSHVHPKLPDYEEIADKFIALKRGHMQQQRKQ 587
             S  TKCMK A KYPSHVHPKLPDY+EIA +FI+LKR ++Q+  K+
Sbjct: 541 -PSKFTKCMKGAHKYPSHVHPKLPDYDEIAVEFISLKREYLQRSMKR 514

BLAST of Lag0015070 vs. ExPASy TrEMBL
Match: A0A0A0K3R2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G047390 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 3.5e-173
Identity = 350/572 (61.19%), Postives = 405/572 (70.80%), Query Frame = 0

Query: 15  KKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLMEAYDL 74
           KKLIK ALCRL++L+KKRYSIV+ LREDL ELINNGYQQIAF RVEQLI+DE+LMEAYDL
Sbjct: 20  KKLIKTALCRLKLLRKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLIQDETLMEAYDL 79

Query: 75  IESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERYGRSF 134
           IE+FCE ILVKFSHIRKHKTCPDDI EAISSLIFASAR GDFPELK VRKLFEER+G+SF
Sbjct: 80  IENFCEIILVKFSHIRKHKTCPDDITEAISSLIFASARFGDFPELKCVRKLFEERFGKSF 139

Query: 135 AVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPAILALEYCPDWHQKQ 194
            VAAVEL PGNLVN QIKEKL+++PV  HEKQRLI+EIARDCF PA+LALE CPDWH+KQ
Sbjct: 140 IVAAVELSPGNLVNKQIKEKLVMQPVPNHEKQRLINEIARDCFHPAVLALENCPDWHKKQ 199

Query: 195 VLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSDIDIVDPLVCQSSTNGNEEVYCNS 254
           VL+NGDQT  + K        +EELE VV S DS+ D           S  G  + +C+S
Sbjct: 200 VLQNGDQTTVKGK------VESEELERVVISLDSTRD-----------SNCGVLDSWCHS 259

Query: 255 ALASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFREETIIYLDDVIELPN 314
             +S ++                          N AS+ EF PF EE ++Y DDV+EL +
Sbjct: 260 TSSSPSV---------------------SQSFPNDASTSEFLPFCEEAMVYFDDVVELSD 319

Query: 315 PSTEDGDLWDQRFFKFRSPVTSMR-DNVQHGNDQSLIENHDVSNKKSVSERSIQSINGAQ 374
           PSTE GDL DQRFFKF+S +TS R +NV  G+DQSLIENH  SNKK+VS RS Q ING+ 
Sbjct: 320 PSTEYGDLLDQRFFKFKSLITSTREENVGDGDDQSLIENHYGSNKKAVSGRSNQIINGSP 379

Query: 375 KGSRRRSTCRERENHSSSHTKKKLTKCCCPSCHSLLSEMENYCLEQQCYVYSECRTDDYE 434
           K   RRS  RE+ENHS +HTKKKL KC C SCHSL SE++NYC+EQQCYV+SECRT    
Sbjct: 380 KEPSRRSMRREQENHSLNHTKKKLMKCYCLSCHSLSSELQNYCVEQQCYVHSECRT---- 439

Query: 435 VVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDEELEFDTFSMTKKMR 494
                    D   I+SS  N  DYDLS K+         WN +S+EE+EF TFS  K  R
Sbjct: 440 ---------DFSVIQSSEANNSDYDLSSKNL--------WNAESNEEIEFITFSRAKDQR 499

Query: 495 SCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNGMESNITKCMKAADK 554
           +  IGTVVYDVFVYSQC+PDEN ETN K +E  T GK E         S  TKCMK ADK
Sbjct: 500 NYGIGTVVYDVFVYSQCQPDENKETNTKLKELSTNGKHE--------PSKFTKCMKGADK 524

Query: 555 YPSHVHPKLPDYEEIADKFIALKRGHMQQQRK 586
           YPSHVHPKLP+YEEIA KFI LKR ++++  K
Sbjct: 560 YPSHVHPKLPNYEEIAAKFICLKREYLERSMK 524

BLAST of Lag0015070 vs. ExPASy TrEMBL
Match: A0A5D3C6Z3 (Ist1 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003520 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 6.1e-170
Identity = 349/573 (60.91%), Postives = 400/573 (69.81%), Query Frame = 0

Query: 15  KKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLMEAYDL 74
           KKLIK ALCRL++LKKKRYSIV+ LREDL ELINNGYQQIAF RVEQL++DE+LME YDL
Sbjct: 4   KKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDL 63

Query: 75  IESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERYGRSF 134
           IE+ CEFILVKFSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK VR LFEER+G+SF
Sbjct: 64  IENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSF 123

Query: 135 AVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPAILALEYCPDWHQKQ 194
           AVAAVEL PGNLVN QIKEKL+++ V  HEKQRLI+EIARDCF PA+LALEY PD  QKQ
Sbjct: 124 AVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQ 183

Query: 195 VLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSDIDIVDPLVCQSSTNGNEEVYCNS 254
           VL N DQT  E K        +EELE VV S DS+ D           S  G  + +C+S
Sbjct: 184 VLPNEDQTTVEGK------VESEELERVVISLDSARD-----------SNCGVLDPWCHS 243

Query: 255 ALASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFSPFREETIIYLDDVIELPN 314
             +SS++       C S                N ASS EF PF EE I+Y DDV+EL +
Sbjct: 244 TSSSSSV-------CQSF--------------PNDASSSEFLPFCEEAIVYFDDVVELSD 303

Query: 315 PSTEDGDLWDQRFFKFRSPVTSMR-DNVQHGNDQSLIENHDVSNKKSVSERSIQSINGAQ 374
           PSTE GDL DQRFFKF+  +TS R +NV+  +DQSLIE HD SNKKSVS RS Q ING  
Sbjct: 304 PSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIEKHDASNKKSVSGRSNQIINGPP 363

Query: 375 KGSRRRSTCRERENHSSSHTKKKLTKCCCPSCHSLLSEMENYCLEQQCYVYSECRTDDYE 434
           K   R+  CRE+ENHS +HTKKK TKCCC SCHSL  E++NY LEQQCYVYSECRT    
Sbjct: 364 KEPSRKLMCREQENHSLNHTKKKFTKCCCLSCHSLSFELQNYSLEQQCYVYSECRT---- 423

Query: 435 VVISEKKKADLYFIRSSADNRFDYDLSCKDSPPNKDHIPWNGDSDEELEFDTFSMTKKMR 494
                    D Y IRSS  NR DYDLS K       H+ WN +S+EE+EF TFS  K+ R
Sbjct: 424 ---------DYYIIRSSEANRLDYDLSSK-------HL-WNAESNEEIEFITFSRAKEQR 483

Query: 495 SCEIGTVVYDVFVYSQCKPDENMETNAKPEEFRTKGKQESSFDFNGMESNITKCMKAADK 554
           +  IGTVVYDVFVYS C+PDEN ETN K EE                 S  TKCMK A K
Sbjct: 484 NYGIGTVVYDVFVYSHCQPDENKETNTKLEEHE--------------PSKFTKCMKGAHK 503

Query: 555 YPSHVHPKLPDYEEIADKFIALKRGHMQQQRKQ 587
           YPSHVHPKLPDY+EIA +FI+LKR ++Q+  K+
Sbjct: 544 YPSHVHPKLPDYDEIAVEFISLKREYLQRSMKR 503

BLAST of Lag0015070 vs. ExPASy TrEMBL
Match: A0A6J1GM59 (uncharacterized protein LOC111455514 OS=Cucurbita moschata OX=3662 GN=LOC111455514 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.3e-90
Identity = 166/200 (83.00%), Postives = 186/200 (93.00%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVE 60
           MFGILFGWRKASKCKKL+++ALCRL+MLKKKRYSI RQLREDLFELI+NGYQQIAFNRVE
Sbjct: 1   MFGILFGWRKASKCKKLVEQALCRLKMLKKKRYSIARQLREDLFELISNGYQQIAFNRVE 60

Query: 61  QLIRDESLMEAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 120
           QL+RDESLMEAYDLIE+FCEFILVKFSH+RK+KTCPDD++EAISSLIFASARCGDFPELK
Sbjct: 61  QLVRDESLMEAYDLIENFCEFILVKFSHVRKNKTCPDDLMEAISSLIFASARCGDFPELK 120

Query: 121 PVRKLFEERYGRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPA 180
           PVRK FEERYG+ FA+AAVEL P NLVNPQIKEKL +K V + EKQRL++EIARDCF PA
Sbjct: 121 PVRKFFEERYGQRFAMAAVELHPENLVNPQIKEKLAMKAVSDDEKQRLMNEIARDCFHPA 180

Query: 181 ILALEYCPDWHQKQVLENGD 201
           ILALEYCPDW+Q+  L+  D
Sbjct: 181 ILALEYCPDWYQEHGLDRTD 200

BLAST of Lag0015070 vs. TAIR 10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 183.7 bits (465), Expect = 4.2e-46
Identity = 100/250 (40.00%), Postives = 154/250 (61.60%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVE 60
           MF  LFGWR+ SKCK ++K+  CRL +LK K+Y+I   LR D+ +L+  G +  A +R +
Sbjct: 71  MFCFLFGWRRTSKCKSVVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRAQ 130

Query: 61  QLIRDESLMEAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 120
           QL  DE+LM  Y L+  F + IL+  S+IR+ +  PD I EA+S+L+FASARCGD PEL+
Sbjct: 131 QLFLDENLMSLYHLLLHFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPELR 190

Query: 121 PVRKLFEERYGRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDC-FQP 180
            +R LF +RYG  F   A+ L PGN VNPQ+ EKL +  V +  K +L+ EI  +   + 
Sbjct: 191 ALRVLFGKRYGNHFVDTALNLLPGNCVNPQVIEKLSIISVSDDAKSKLLGEIVEEYNLRL 250

Query: 181 AILALEYCPDWHQKQVLENGDQTHCERKEPKVQDSNAEELETVVSSRDSSSDIDIVDPLV 240
            +LA+EY P++H KQVL++      E+ E + +  ++   +   SS+ + S+ ++    +
Sbjct: 251 EVLAIEYTPEFH-KQVLKS------EKAEEEKEVMSSNSAQPYCSSQKAESEAEVYKFTL 310

Query: 241 CQSSTNGNEE 250
             +    N+E
Sbjct: 311 TDADIEENQE 313

BLAST of Lag0015070 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 125.9 bits (315), Expect = 1.0e-28
Identity = 86/262 (32.82%), Postives = 138/262 (52.67%), Query Frame = 0

Query: 10  KASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLM 69
           KA+KCK L+K  + R+++++ +R + ++Q+R ++ +L+  G +  A  RVE +IR+E +M
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 70  EAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEER 129
            A +++E FCE I V+   I   + CP D+ EAISS+ FA+ RC D  EL+ V+ LF  +
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 130 YGRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARD---CFQPAILALEY 189
           YG+ F  AA EL P + VN ++ E L V+      K +L+ EIA +    + PA    + 
Sbjct: 133 YGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDL 192

Query: 190 CPDWHQKQVLENGDQTHCERKEPKVQDSNAE----ELETVVSSRDSSSDIDIVD----PL 249
                 + +L+   Q     K P  ++ N +     L       DS S+ DI+D    P 
Sbjct: 193 FKS--HEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDSEYDILDFPEVPN 252

Query: 250 VCQSSTNGNEEVYCNSALASSN 261
           V    T G   V    A  S++
Sbjct: 253 VLLRPTPGATSVNAPDAAKSAS 272

BLAST of Lag0015070 vs. TAIR 10
Match: AT1G51900.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 124.0 bits (310), Expect = 3.9e-28
Identity = 75/192 (39.06%), Postives = 115/192 (59.90%), Query Frame = 0

Query: 1   MFGILFGWRKASKC--KKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNR 60
           MFG  F  R+ S     K++K+   RL +LK ++Y+  R LR D+ + I +   + A  R
Sbjct: 1   MFGFSFSRRRKSNAFTWKVLKQLQSRLMLLKSQKYAKSRHLRADIVDFIRSNDSKSALFR 60

Query: 61  VEQLIRDESLMEAYDLIESFCEFILVKFSHIRKHK--TCPDDIIEAISSLIFASARCGDF 120
            EQL+  E+ +  Y  +  F +FIL++FS  +KH      DD  EA+SSLIFAS +C + 
Sbjct: 61  TEQLLLVENAITIYGFLLKFTDFILLRFSPSKKHSCLLVNDDTSEAVSSLIFASVKCREI 120

Query: 121 PELKPVRKLFEERYGRSFAVAAVELCPGNLVNPQIKEKLLVKPVL-EHEKQRLISEIARD 180
           PEL  + +L  +RYG+ +   A+++ PGNLVN +IKEKL    V+ E +K R++ EIA++
Sbjct: 121 PELLIISELVGQRYGQRYVTTAIQVPPGNLVNTEIKEKLKSTSVVSETDKCRVMEEIAKE 180

Query: 181 C-FQPAILALEY 187
             ++  IL L Y
Sbjct: 181 SGYRLEILGLGY 192

BLAST of Lag0015070 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 122.1 bits (305), Expect = 1.5e-27
Identity = 88/292 (30.14%), Postives = 156/292 (53.42%), Query Frame = 0

Query: 11  ASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVEQLIRDESLME 70
           +SKCK   K A+ R+++++ KR  +V+Q+R D+  L+ +G    A  RVE +IR++++  
Sbjct: 28  SSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQA 87

Query: 71  AYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKPVRKLFEERY 130
           A ++IE FCE I+ + + I K K CP D+ E I+SLIFA+ RC + PEL  +R +F ++Y
Sbjct: 88  ANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKY 147

Query: 131 GRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPAILALEYCPDW 190
           G+ F  AA +L P   VN  + +KL V+      K +++ EIA+          E+  DW
Sbjct: 148 GKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAK----------EFQVDW 207

Query: 191 HQKQVLENGDQTHCERKEPKVQDSNAEELETVVSSR----DSSSDIDIVDP--LVCQSST 250
                    D T  E++  K Q+ + +     VS+     + ++  + +DP   V +S++
Sbjct: 208 ---------DTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTS 267

Query: 251 NGNEEVYCNSALASSNLLSWQAKECDSSGWIAAATLEDGLDRGNGASSPEFS 297
           + +   + +   +++   +  AK+      +AAA +   L     +S+ EFS
Sbjct: 268 SMSINTHYHDTESAAEAATELAKQA-----VAAAQVASLLATRRDSSNKEFS 295

BLAST of Lag0015070 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 121.3 bits (303), Expect = 2.6e-27
Identity = 73/228 (32.02%), Postives = 117/228 (51.32%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKRALCRLQMLKKKRYSIVRQLREDLFELINNGYQQIAFNRVE 60
           MF  LF  +  +KCK L+K    R+  +K+K+ S+ + L+ D+ +L+ N     A+ R E
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  QLIRDESLMEAYDLIESFCEFILVKFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 120
            LI ++  +  Y+ +E FC  +    S ++K   CPD+  EAISSL++A+AR  + PEL+
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 PVRKLFEERYGRSFAVAAVELCPGNLVNPQIKEKLLVKPVLEHEKQRLISEIARDCFQPA 180
            +R LF ERYG +             VNP+  E+   +P  +  K  L+ EIAR      
Sbjct: 121 DLRSLFAERYGNTL---------DQFVNPEFVERFKAEPPSKEMKVELLQEIAR------ 180

Query: 181 ILALEYCPDWHQKQVLE------NGDQTHCERKEPKVQDSNAEELETV 223
               EY   W  K + +      +    H E +EPK + +N+E  +T+
Sbjct: 181 ----EYSIKWDAKSLEQRLYTPPHHHHVHTETEEPKTEKTNSEPEKTM 209